Rh5AG008200

Auxin-induced in root cultures protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012068
Physical Location & Seq
Forward (+)
562929 .. 566163
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG008200.1

Sequence Viewer

Length: 966 bp
ATGGCAGCCATGTTCAAGCTTCTCACAGCTTTCTGTGTTTTGGTCTCTCTATCTTCCTTCTCAGATGCGCAAACATGTAAGAGCCAGACGTTCACCAGCAACCGAGCCTTCACTTCCTGCAGCGACCTTCCATACCTCAACGCCTTCCTCCACTGGACCTTTGAGCAGTCCACCGGGAAGCTCACGATGGCGTACAGGCACACGTCGGCGTCAGACACCAACTGGGTCGCCTGGGCCATCAACCCGGACTTGACCAAGACCCAGAAGGCCATGACCGGCGCGCAAGCTCTTGTTGCTATTCCTCAAACCAGTGGGGCCCCTTCAGTCCGCACGTCTCCGATCACCGGATACGGCACGACTTTGGCGAACGGAACTTTGAGTTACAATGTGAGCGGTGTATCCGCTGAGTCTCAGAACGGTGAGGTGATCATCTACGCCACTTGGACCCTGCCGACGACTACGACGACGATTCTTCAGGTTTGGCAGGAGGGTTCGGTTGTGAGTGGGAACCCCCAACAACACGGAAGCGATGCCGCCAATCTTAATGCCCAGGGGACTTTGGACTTGCTTTCCGGCCAGACCGCCGCTTCCGGTGGAGCCAGCTCCACACTCAGGAAGAAAAACATTCACGCAGTGCTAAATGTAGTGAGTTGGGGTATCTTGATGCCTGTAGGAGCCATGGTAGCAAGGTACTTGAAGACATTCAAGGCTGCAGACCCGGCATGGTTTTACCTCCACGCCGGGTGCCAGACCTCTGCTTATATTGTTGGTGTTGCTGGATGGGCCACAGGTATTAAGCTCGGTAGCGATTCTGCCGGTATCCAATATGATACTCACAGAAACATTGGGATTACCCTCTTCTGCCTTGGAACACTTCAGGTACTCTCATCTTTAAACACACTGACATGTTGTTTCACAATGTTTGTAGTGGTTTGGCCACATGTCCTCTATGGTTATACTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

321

Amino Acids

34.25

Weight (kDa)

8.64

Isoelectric Point (pI)

20.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 91 - 189 3.1e-25 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17280 AT5G47530
fragaria_vesca FvH4_3g00780 FvH4_3g00780
malus_domestica MD05G1359500.v1.1 MD10G1335000.v1.1
prunus_persica Prupe.4G008600_v2.0.a1
pyrus_communis pycom10g28480
rosa_chinensis RchiOBHm_Chr5g0001021
rosa_laevigata RLG00000030914
rosa_multiflora Rmu_sc0000255.1_g000030 Rmu_sc0004189.1_g000001
rosa_roxburghii Rroxscaffold_1G00075260
rosa_rugosa Rorug04G0389000
rosa_samantha Rh5AG008200 Rh5BG010300 Rh5CG008900 Rh5DG009000
rosa_wichuraiana Rw5G000840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 69
AccB1I GGYRCC 1 cut(s) 744
AccBSI CCGCTC 1 cut(s) 393
AccII CGCG 1 cut(s) 281
AciI CCGC 6 cut(s) 328, 393, 402, 534, 582, 585
AcoI YGGCCR 2 cut(s) 574, 935
AcuI CTGAAG 3 cut(s) 306, 458, 860
AcyI GRCGYC 1 cut(s) 209
AdeI CACNNNGTG 1 cut(s) 634
AfaI GTAC 3 cut(s) 194, 692, 882
AfiI CCNNNNNNNGG 2 cut(s) 344, 612
AflIII ACRYGT 4 cut(s) 74, 201, 905, 940
AgsI TTSAA 3 cut(s) 16, 697, 706
AjiI CACGTC 2 cut(s) 204, 333
AjnI CCWGG 2 cut(s) 230, 549
AluBI AGCT 6 cut(s) 19, 29, 181, 287, 603, 799
AluI AGCT 6 cut(s) 19, 29, 181, 287, 603, 799
Alw26I GTCTC 3 cut(s) 49, 339, 414
AoxI GGCC 6 cut(s) 234, 267, 315, 574, 783, 935
ApaI GGGCCC 1 cut(s) 319
ApeKI GCWGC 3 cut(s) 5, 120, 710
Asp700I GAANNNNTTC 1 cut(s) 701
AspLEI GCGC 3 cut(s) 70, 281, 283
AspS9I GGNCC 6 cut(s) 156, 234, 315, 316, 444, 783
AsuC2I CCSGG 4 cut(s) 175, 245, 719, 742
AsuHPI GGTGA 4 cut(s) 85, 334, 431, 436
AvaII GGWCC 2 cut(s) 156, 444
BaeGI GKGCMC 1 cut(s) 319
BalI TGGCCA 1 cut(s) 937
BanI GGYRCC 1 cut(s) 744
BanII GRGCYC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 704
BbvI GCAGC 3 cut(s) 17, 132, 697
BccI CCATC 3 cut(s) 181, 245, 774
BceAI ACGGC 1 cut(s) 367
BciT130I CCWGG 2 cut(s) 232, 551
BciVI GTATCC 3 cut(s) 341, 409, 830
BclI TGATCA 1 cut(s) 426
BcnI CCSGG 4 cut(s) 175, 245, 719, 742
BcoDI GTCTC 3 cut(s) 49, 339, 414
BfmI CTRYAG 3 cut(s) 118, 669, 711
BfuI GTATCC 3 cut(s) 341, 409, 830
BisI GCNGC 5 cut(s) 6, 121, 534, 585, 711
BlsI GCNGC 5 cut(s) 7, 122, 535, 586, 712
Bme1390I CCNGG 6 cut(s) 175, 232, 245, 551, 719, 742
Bme18I GGWCC 2 cut(s) 156, 444
BmgBI CACGTC 2 cut(s) 204, 333
BmgT120I GGNCC 6 cut(s) 156, 234, 315, 316, 444, 783
BmiI GGNNCC 8 cut(s) 316, 317, 318, 446, 509, 598, 676, 746
BmrFI CCNGG 6 cut(s) 175, 232, 245, 551, 719, 742
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 3 cut(s) 55, 520, 654
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 704
BpuMI CCSGG 4 cut(s) 175, 245, 719, 742
BsaHI GRCGYC 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 5 cut(s) 231, 549, 550, 678, 865
BsaWI WCCGGW 2 cut(s) 344, 590
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 612
Bse118I RCCGGY 2 cut(s) 275, 815
Bse1I ACTGG 3 cut(s) 158, 227, 309
BseBI CCWGG 2 cut(s) 232, 551
BseDI CCNNGG 5 cut(s) 231, 549, 550, 678, 865
BseGI GGATG 1 cut(s) 785
BseLI CCNNNNNNNGG 2 cut(s) 344, 612
BseMII CTCAG 4 cut(s) 75, 396, 425, 625
BseNI ACTGG 3 cut(s) 158, 227, 309
BsePI GCGCGC 1 cut(s) 279
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 3 cut(s) 17, 132, 697
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 6 cut(s) 236, 269, 317, 576, 785, 937
BshNI GGYRCC 1 cut(s) 744
BsiSI CCGG 9 cut(s) 174, 245, 276, 345, 573, 591, 719, 741, 816
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 2 cut(s) 344, 612
BsmAI GTCTC 3 cut(s) 49, 339, 414
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 568
BsnI GGCC 6 cut(s) 236, 269, 317, 576, 785, 937
Bso31I GGTCTC 1 cut(s) 49
Bsp120I GGGCCC 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 2 cut(s) 339, 426
Bsp19I CCATGG 1 cut(s) 678
BspACI CCGC 6 cut(s) 328, 393, 402, 534, 582, 585
BspANI GGCC 6 cut(s) 236, 269, 317, 576, 785, 937
BspCNI CTCAG 4 cut(s) 74, 397, 424, 624
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 8 cut(s) 316, 317, 318, 446, 509, 598, 676, 746
BspMAI CTGCAG 2 cut(s) 122, 715
BspT107I GGYRCC 1 cut(s) 744
BspTNI GGTCTC 1 cut(s) 49
BsrBI CCGCTC 1 cut(s) 393
BsrFI RCCGGY 2 cut(s) 275, 815
BsrI ACTGG 3 cut(s) 158, 227, 309
BssAI RCCGGY 2 cut(s) 275, 815
BssECI CCNNGG 5 cut(s) 231, 549, 550, 678, 865
BssHII GCGCGC 1 cut(s) 279
BssMI GATC 2 cut(s) 339, 426
BssNI GRCGYC 1 cut(s) 209
BssT1I CCWWGG 2 cut(s) 678, 865
Bst2UI CCWGG 2 cut(s) 232, 551
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 1 cut(s) 863
BstACI GRCGYC 1 cut(s) 209
BstC8I GCNNGC 3 cut(s) 281, 285, 601
BstDEI CTNAG 5 cut(s) 61, 405, 411, 611, 960
BstDSI CCRYGG 1 cut(s) 678
BstF5I GGATG 1 cut(s) 785
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 3 cut(s) 70, 281, 283
BstKTI GATC 2 cut(s) 342, 429
BstMAI GTCTC 3 cut(s) 49, 339, 414
BstMBI GATC 2 cut(s) 339, 426
BstMWI GCNNNNNNNGC 4 cut(s) 293, 683, 719, 782
BstNI CCWGG 2 cut(s) 232, 551
BstNSI RCATGY 3 cut(s) 78, 909, 944
BstSCI CCNGG 6 cut(s) 173, 230, 243, 549, 717, 740
BstSFI CTRYAG 3 cut(s) 118, 669, 711
BstSLI GKGCMC 1 cut(s) 319
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 3 cut(s) 17, 132, 697
BstV2I GAAGAC 1 cut(s) 704
BsuI GTATCC 3 cut(s) 341, 409, 830
BsuRI GGCC 6 cut(s) 236, 269, 317, 576, 785, 937
BtgI CCRYGG 1 cut(s) 678
BtgZI GCGATG 1 cut(s) 543
BtrI CACGTC 2 cut(s) 204, 333
BtsCI GGATG 1 cut(s) 785
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 4 cut(s) 151, 316, 639, 899
Cac8I GCNNGC 3 cut(s) 281, 285, 601
CfoI GCGC 3 cut(s) 70, 281, 283
Cfr10I RCCGGY 2 cut(s) 275, 815
Cfr13I GGNCC 6 cut(s) 156, 234, 315, 316, 444, 783
CseI GACGC 1 cut(s) 198
Csp6I GTAC 3 cut(s) 193, 691, 881
CviAII CATG 7 cut(s) 10, 75, 271, 679, 723, 906, 941
CviQI GTAC 3 cut(s) 193, 691, 881
DdeI CTNAG 5 cut(s) 61, 405, 411, 611, 960
DpnI GATC 2 cut(s) 341, 428
DpnII GATC 2 cut(s) 339, 426
DraI TTTAAA 1 cut(s) 894
DraIII CACNNNGTG 1 cut(s) 634
EaeI YGGCCR 2 cut(s) 574, 935
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
Eco130I CCWWGG 2 cut(s) 678, 865
Eco24I GRGCYC 1 cut(s) 319
Eco31I GGTCTC 1 cut(s) 49
Eco47I GGWCC 2 cut(s) 156, 444
Eco57I CTGAAG 3 cut(s) 306, 458, 860
EcoO109I RGGNCCY 2 cut(s) 315, 316
EcoRII CCWGG 2 cut(s) 230, 549
EcoT14I CCWWGG 2 cut(s) 678, 865
EcoT38I GRGCYC 1 cut(s) 319
ErhI CCWWGG 2 cut(s) 678, 865
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 7 cut(s) 13, 78, 274, 682, 726, 909, 944
FaqI GGGAC 1 cut(s) 568
FatI CATG 7 cut(s) 9, 74, 270, 678, 722, 905, 940
FbaI TGATCA 1 cut(s) 426
Fnu4HI GCNGC 5 cut(s) 6, 121, 534, 585, 711
FokI GGATG 1 cut(s) 792
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 5 cut(s) 6, 121, 534, 585, 711
FspI TGCGCA 1 cut(s) 69
GlaI GCGC 3 cut(s) 69, 280, 282
GluI GCNGC 5 cut(s) 6, 121, 534, 585, 711
HaeIII GGCC 6 cut(s) 236, 269, 317, 576, 785, 937
HapII CCGG 9 cut(s) 174, 245, 276, 345, 573, 591, 719, 741, 816
HgaI GACGC 1 cut(s) 198
HhaI GCGC 3 cut(s) 70, 281, 283
Hin1I GRCGYC 1 cut(s) 209
Hin1II CATG 7 cut(s) 13, 78, 274, 682, 726, 909, 944
Hin6I GCGC 3 cut(s) 68, 279, 281
HinP1I GCGC 3 cut(s) 68, 279, 281
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 3 cut(s) 407, 469, 809
HpaII CCGG 9 cut(s) 174, 245, 276, 345, 573, 591, 719, 741, 816
HphI GGTGA 4 cut(s) 85, 334, 431, 436
Hpy166II GTNNAC 2 cut(s) 93, 171
Hpy188I TCNGA 4 cut(s) 64, 214, 339, 414
Hpy188III TCNNGA 3 cut(s) 184, 613, 661
Hpy8I GTNNAC 2 cut(s) 93, 171
Hpy99I CGWCG 4 cut(s) 208, 457, 466, 469
HpyAV CCTTC 6 cut(s) 67, 118, 137, 154, 259, 330
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4IV ACGT 3 cut(s) 89, 203, 332
HpyCH4V TGCA 2 cut(s) 120, 713
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 683, 719, 782
HpyF3I CTNAG 5 cut(s) 61, 405, 411, 611, 960
HpySE526I ACGT 3 cut(s) 89, 203, 332
Hsp92I GRCGYC 1 cut(s) 209
Hsp92II CATG 7 cut(s) 13, 78, 274, 682, 726, 909, 944
HspAI GCGC 3 cut(s) 68, 279, 281
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 2 cut(s) 339, 426
LmnI GCTCC 3 cut(s) 596, 608, 674
Lsp1109I GCAGC 3 cut(s) 17, 132, 697
LweI GCATC 3 cut(s) 55, 520, 654
MaeII ACGT 3 cut(s) 89, 203, 332
MaeIII GTNAC 1 cut(s) 380
MalI GATC 2 cut(s) 341, 428
MbiI CCGCTC 1 cut(s) 393
MboI GATC 2 cut(s) 339, 426
MboII GAAGA 5 cut(s) 45, 464, 628, 709, 850
MhlI GDGCHC 1 cut(s) 319
MlsI TGGCCA 1 cut(s) 937
MluNI TGGCCA 1 cut(s) 937
MlyI GAGTC 1 cut(s) 416
MnlI CCTC 9 cut(s) 146, 158, 312, 415, 481, 743, 763, 866, 956
Mox20I TGGCCA 1 cut(s) 937
MroXI GAANNNNTTC 1 cut(s) 701
MscI TGGCCA 1 cut(s) 937
MseI TTAA 3 cut(s) 543, 795, 893
MslI CAYNNNNRTG 1 cut(s) 904
Msp20I TGGCCA 1 cut(s) 937
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 9 cut(s) 174, 245, 276, 345, 573, 591, 719, 741, 816
MspR9I CCNGG 6 cut(s) 175, 232, 245, 551, 719, 742
MvaI CCWGG 2 cut(s) 232, 551
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 4 cut(s) 293, 683, 719, 782
NciI CCSGG 4 cut(s) 175, 245, 719, 742
NcoI CCATGG 1 cut(s) 678
NdeII GATC 2 cut(s) 339, 426
NlaIII CATG 7 cut(s) 13, 78, 274, 682, 726, 909, 944
NlaIV GGNNCC 8 cut(s) 316, 317, 318, 446, 509, 598, 676, 746
NsbI TGCGCA 1 cut(s) 69
NspI RCATGY 3 cut(s) 78, 909, 944
PasI CCCWGGG 1 cut(s) 550
PauI GCGCGC 1 cut(s) 279
PciI ACATGT 3 cut(s) 74, 905, 940
PcsI WCGNNNNNNNCGW 2 cut(s) 362, 461
PdmI GAANNNNTTC 1 cut(s) 701
PfeI GAWTC 2 cut(s) 469, 809
PkrI GCNGC 5 cut(s) 7, 122, 535, 586, 712
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PscI ACATGT 3 cut(s) 74, 905, 940
Psp6I CCWGG 2 cut(s) 230, 549
PspGI CCWGG 2 cut(s) 230, 549
PspN4I GGNNCC 8 cut(s) 316, 317, 318, 446, 509, 598, 676, 746
PspOMI GGGCCC 1 cut(s) 315
PspPI GGNCC 6 cut(s) 156, 234, 315, 316, 444, 783
PstI CTGCAG 2 cut(s) 122, 715
PteI GCGCGC 1 cut(s) 279
RsaI GTAC 3 cut(s) 194, 692, 882
RsaNI GTAC 3 cut(s) 193, 691, 881
RseI CAYNNNNRTG 1 cut(s) 904
SaqAI TTAA 3 cut(s) 543, 795, 893
SatI GCNGC 5 cut(s) 6, 121, 534, 585, 711
Sau3AI GATC 2 cut(s) 339, 426
Sau96I GGNCC 6 cut(s) 156, 234, 315, 316, 444, 783
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 6 cut(s) 175, 232, 245, 551, 719, 742
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 3 cut(s) 55, 520, 654
SfcI CTRYAG 3 cut(s) 118, 669, 711
SinI GGWCC 2 cut(s) 156, 444
SmiMI CAYNNNNRTG 1 cut(s) 904
SsiI CCGC 6 cut(s) 328, 393, 402, 534, 582, 585
StyD4I CCNGG 6 cut(s) 173, 230, 243, 549, 717, 740
StyI CCWWGG 2 cut(s) 678, 865
TaaI ACNGT 1 cut(s) 419
TaiI ACGT 3 cut(s) 92, 206, 335
TauI GCSGC 2 cut(s) 536, 587
TfiI GAWTC 2 cut(s) 469, 809
Tru1I TTAA 3 cut(s) 543, 795, 893
Tru9I TTAA 3 cut(s) 543, 795, 893
TscAI CASTG 4 cut(s) 158, 316, 639, 906
TseI GCWGC 3 cut(s) 5, 120, 710
TspGWI ACGGA 2 cut(s) 384, 537
TspRI CASTG 4 cut(s) 158, 316, 639, 906
VpaK11BI GGWCC 2 cut(s) 156, 444
XceI RCATGY 3 cut(s) 78, 909, 944
XmnI GAANNNNTTC 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.