FvH4_3g04360

S1/P1 Nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012928
Physical Location & Seq
Forward (+)
2490330 .. 2493176
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04360.t2

Sequence Viewer

Length: 963 bp
ATGGATACTTATAGAGGCTTCGGCAGATTTGCCAACCATTTTCTTGAGTTTGTTTTGTTGCTCGCTTTTGTTTGCATTTTAATTCCGAAAGCTCAGGGATGGAGCAAAGAGGGTCACATCATGACTTGCCGGATTGCACAGGCACTTCTAGAGCCTGAAGCTGCAGAAGCTGTTGAAAATTTACTACCTCATGATGTCAATGGCGATTTGTCGGCTCTGTGTGTGTGGCCTGACCAGATCAGGCACTGGTACAGATACCGGTGGACTAGCCCTCTTCACTTCATTGACACACCTGATAATGCTTGCAACTTTGAGTACTCAAGAGATTGCCATGATCAACATGGCTCCAAGGACATGTGTGTTGCTGGTGCAGTCCAGAATTTCACATCTCAGCTTTTGCACTACACAGAAGGAACATCTGACCGTCGATATAATATGACTGAGGCCTTGCTATTCTTGTCACACTTTATGGGTGATCTCCATCAGCCTATGCATGTCGGATTCACGACTGATGAAGGAGGAAACACAATAGACTTGCGTTGGTACAAGCACAAATCCAATCTCCACCATGTATGGGATCGAGAGATTATAATGCAAGCTCTGCATGATTATTATGACAAGGACATGGAACTCCTCCTGCAAGACATACAGGGAAACATCACAGTTGGGGTCTGGTCTGATGATGTTACATCATGGGGACATTGTGATGAAATCAGTTCATGTATAAACAAGTATGCTACAGAAAGTATGAACATAGCTTGCAAATGGGGTTACAAGGGCGTTGAGTCCGGTGATACTCTAACAGAGGATTACTTCCTGCCCCGGCTACCTATTGTGGAGAAGAGGATTGCCCAAGGTGGAGTCCGGTTGGCTGCTATCCTCAATCGTGCCTTCTCCTCTAAATCTCAAGCACAACCTGCTGATCATCACAAAAACAAAAGAGCATCACATTCACCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1-P1_nuclease

Protein Analysis

321

Amino Acids

36.66

Weight (kDa)

5.84

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 34 - 221 4.3e-63 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11190
fragaria_vesca FvH4_3g04360 FvH4_3g04360
malus_domestica MD05G1323600.v1.1 MD10G1079400.v1.1 MD10G1301500.v1.1
prunus_persica Prupe.4G038900_v2.0.a1
pyrus_communis pycom10g25420
rosa_chinensis RchiOBHm_Chr5g0006751
rosa_laevigata RLG00000031425
rosa_multiflora Rmu_sc0001044.1_g000042
rosa_roxburghii Rroxscaffold_1G00069290
rosa_rugosa Rorug04G0424200
rosa_samantha Rh5AG052100 Rh5BG051000 Rh5CG059600 Rh5DG050100
rosa_wichuraiana Rw5G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
Acc36I ACCTGC 1 cut(s) 925
AclWI GGATC 1 cut(s) 585
AcsI RAATTY 2 cut(s) 178, 379
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 3 cut(s) 251, 317, 545
AfiI CCNNNNNNNGG 1 cut(s) 574
AflIII ACRYGT 1 cut(s) 354
AgeI ACCGGT 1 cut(s) 258
AgsI TTSAA 1 cut(s) 176
AluBI AGCT 6 cut(s) 92, 161, 170, 394, 599, 758
AluI AGCT 6 cut(s) 92, 161, 170, 394, 599, 758
AlwI GGATC 1 cut(s) 585
AlwNI CAGNNNCTG 2 cut(s) 170, 246
AoxI GGCC 2 cut(s) 227, 444
ApeKI GCWGC 2 cut(s) 161, 872
ApoI RAATTY 2 cut(s) 178, 379
AsiGI ACCGGT 1 cut(s) 258
AsuC2I CCSGG 1 cut(s) 823
AsuHPI GGTGA 3 cut(s) 485, 803, 945
BbvI GCAGC 2 cut(s) 148, 859
BccI CCATC 2 cut(s) 93, 489
BclI TGATCA 2 cut(s) 334, 922
BcnI CCSGG 1 cut(s) 823
BfaI CTAG 2 cut(s) 149, 267
BfmI CTRYAG 2 cut(s) 162, 738
BfuAI ACCTGC 1 cut(s) 925
BisI GCNGC 2 cut(s) 162, 873
BlsI GCNGC 2 cut(s) 163, 874
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 1 cut(s) 823
BmiI GGNNCC 1 cut(s) 346
BmrFI CCNGG 1 cut(s) 823
BmsI GCATC 1 cut(s) 953
Bpu10I CCTNAGC 1 cut(s) 93
BpuEI CTTGAG 3 cut(s) 65, 304, 891
BpuMI CCSGG 1 cut(s) 823
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 3 cut(s) 348, 821, 853
BsaWI WCCGGW 3 cut(s) 258, 788, 864
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 1 cut(s) 251
Bse8I GATNNNNATC 1 cut(s) 480
BseDI CCNNGG 3 cut(s) 348, 821, 853
BseGI GGATG 1 cut(s) 104
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 574
BseMII CTCAG 3 cut(s) 107, 404, 432
BseNI ACTGG 1 cut(s) 251
BseRI GAGGAG 2 cut(s) 623, 886
BseXI GCAGC 2 cut(s) 148, 859
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 2 cut(s) 229, 446
BshTI ACCGGT 1 cut(s) 258
BsiSI CCGG 5 cut(s) 130, 259, 789, 823, 865
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 1 cut(s) 574
BsmFI GGGAC 1 cut(s) 711
BsnI GGCC 2 cut(s) 229, 446
Bsp143I GATC 5 cut(s) 237, 334, 475, 577, 922
BspANI GGCC 2 cut(s) 229, 446
BspCNI CTCAG 3 cut(s) 106, 403, 433
BspHI TCATGA 2 cut(s) 120, 190
BspLI GGNNCC 1 cut(s) 346
BspMAI CTGCAG 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 925
BspPI GGATC 1 cut(s) 585
BsrFI RCCGGY 1 cut(s) 258
BsrI ACTGG 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 3 cut(s) 348, 821, 853
BssMI GATC 5 cut(s) 237, 334, 475, 577, 922
BssT1I CCWWGG 2 cut(s) 348, 853
Bst4CI ACNGT 2 cut(s) 425, 664
Bst6I CTCTTC 2 cut(s) 279, 836
BstAPI GCANNNNNTGC 2 cut(s) 601, 917
BstC8I GCNNGC 4 cut(s) 63, 304, 597, 760
BstDEI CTNAG 4 cut(s) 93, 390, 441, 960
BstF5I GGATG 1 cut(s) 104
BstKTI GATC 5 cut(s) 240, 337, 478, 580, 925
BstMBI GATC 5 cut(s) 237, 334, 475, 577, 922
BstMWI GCNNNNNNNGC 3 cut(s) 167, 601, 917
BstNSI RCATGY 2 cut(s) 358, 497
BstSCI CCNGG 1 cut(s) 821
BstSFI CTRYAG 2 cut(s) 162, 738
BstV1I GCAGC 2 cut(s) 148, 859
BsuRI GGCC 2 cut(s) 229, 446
BtsCI GGATG 1 cut(s) 104
BtsIMutI CAGTG 1 cut(s) 244
BveI ACCTGC 1 cut(s) 925
Cac8I GCNNGC 4 cut(s) 63, 304, 597, 760
CaiI CAGNNNCTG 2 cut(s) 170, 246
CciI TCATGA 2 cut(s) 120, 190
Cfr10I RCCGGY 1 cut(s) 258
Csp6I GTAC 3 cut(s) 250, 316, 544
CspAI ACCGGT 1 cut(s) 258
CviQI GTAC 3 cut(s) 250, 316, 544
DdeI CTNAG 4 cut(s) 93, 390, 441, 960
DpnI GATC 5 cut(s) 239, 336, 477, 579, 924
DpnII GATC 5 cut(s) 237, 334, 475, 577, 922
Eam1104I CTCTTC 2 cut(s) 279, 836
EarI CTCTTC 2 cut(s) 279, 836
Eco130I CCWWGG 2 cut(s) 348, 853
Eco147I AGGCCT 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 177
EcoT14I CCWWGG 2 cut(s) 348, 853
EcoT22I ATGCAT 1 cut(s) 495
ErhI CCWWGG 2 cut(s) 348, 853
FaqI GGGAC 1 cut(s) 711
FbaI TGATCA 2 cut(s) 334, 922
Fnu4HI GCNGC 2 cut(s) 162, 873
FokI GGATG 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 162, 873
FspBI CTAG 2 cut(s) 149, 267
GluI GCNGC 2 cut(s) 162, 873
HaeIII GGCC 2 cut(s) 229, 446
HapII CCGG 5 cut(s) 130, 259, 789, 823, 865
HinfI GANTC 3 cut(s) 501, 785, 861
HpaII CCGG 5 cut(s) 130, 259, 789, 823, 865
HphI GGTGA 3 cut(s) 485, 803, 945
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 4 cut(s) 87, 421, 500, 679
Hpy188III TCNNGA 8 cut(s) 44, 121, 149, 191, 321, 376, 505, 581
Hpy8I GTNNAC 1 cut(s) 264
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 3 cut(s) 404, 509, 901
HpyCH4III ACNGT 2 cut(s) 425, 664
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 601, 917
HpyF3I CTNAG 4 cut(s) 93, 390, 441, 960
Ksp22I TGATCA 2 cut(s) 334, 922
Kzo9I GATC 5 cut(s) 237, 334, 475, 577, 922
LmnI GCTCC 2 cut(s) 102, 350
Lsp1109I GCAGC 2 cut(s) 148, 859
LweI GCATC 1 cut(s) 953
MaeI CTAG 2 cut(s) 149, 267
MaeIII GTNAC 4 cut(s) 113, 459, 685, 770
MalI GATC 5 cut(s) 239, 336, 477, 579, 924
MboI GATC 5 cut(s) 237, 334, 475, 577, 922
MboII GAAGA 2 cut(s) 266, 853
MluCI AATT 3 cut(s) 81, 178, 379
MlyI GAGTC 2 cut(s) 794, 870
MmeI TCCRAC 1 cut(s) 478
Mph1103I ATGCAT 1 cut(s) 495
MseI TTAA 1 cut(s) 80
MslI CAYNNNNRTG 1 cut(s) 705
MspI CCGG 5 cut(s) 130, 259, 789, 823, 865
MspR9I CCNGG 1 cut(s) 823
MwoI GCNNNNNNNGC 3 cut(s) 167, 601, 917
NciI CCSGG 1 cut(s) 823
NdeII GATC 5 cut(s) 237, 334, 475, 577, 922
NlaIV GGNNCC 1 cut(s) 346
NmuCI GTSAC 2 cut(s) 113, 459
NsiI ATGCAT 1 cut(s) 495
NspI RCATGY 2 cut(s) 358, 497
PagI TCATGA 2 cut(s) 120, 190
PceI AGGCCT 1 cut(s) 446
PciI ACATGT 1 cut(s) 354
PfeI GAWTC 1 cut(s) 501
PinAI ACCGGT 1 cut(s) 258
PkrI GCNGC 2 cut(s) 163, 874
PleI GAGTC 2 cut(s) 793, 869
PpsI GAGTC 2 cut(s) 793, 869
PscI ACATGT 1 cut(s) 354
PsiI TTATAA 1 cut(s) 590
PspN4I GGNNCC 1 cut(s) 346
PstI CTGCAG 1 cut(s) 166
PstNI CAGNNNCTG 2 cut(s) 170, 246
RsaI GTAC 3 cut(s) 251, 317, 545
RsaNI GTAC 3 cut(s) 250, 316, 544
RseI CAYNNNNRTG 1 cut(s) 705
SaqAI TTAA 1 cut(s) 80
SatI GCNGC 2 cut(s) 162, 873
Sau3AI GATC 5 cut(s) 237, 334, 475, 577, 922
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 2 cut(s) 794, 870
ScrFI CCNGG 1 cut(s) 823
SfaNI GCATC 1 cut(s) 953
SfcI CTRYAG 2 cut(s) 162, 738
SmiMI CAYNNNNRTG 1 cut(s) 705
SmlI CTYRAG 3 cut(s) 44, 319, 906
SmoI CTYRAG 3 cut(s) 44, 319, 906
Sse9I AATT 3 cut(s) 81, 178, 379
SseBI AGGCCT 1 cut(s) 446
SspMI CTAG 2 cut(s) 149, 267
StuI AGGCCT 1 cut(s) 446
StyD4I CCNGG 1 cut(s) 821
StyI CCWWGG 2 cut(s) 348, 853
TaaI ACNGT 2 cut(s) 425, 664
TaqI TCGA 2 cut(s) 427, 580
TasI AATT 3 cut(s) 81, 178, 379
TatI WGTACW 1 cut(s) 315
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TscAI CASTG 1 cut(s) 251
TseFI GTSAC 2 cut(s) 113, 459
TseI GCWGC 2 cut(s) 161, 872
Tsp45I GTSAC 2 cut(s) 113, 459
TspDTI ATGAA 5 cut(s) 271, 528, 708, 723, 764
TspRI CASTG 1 cut(s) 251
XapI RAATTY 2 cut(s) 178, 379
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 2 cut(s) 358, 497
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XspI CTAG 2 cut(s) 149, 267
ZrmI AGTACT 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.