Prupe.4G038900_v2.0.a1

S1/P1 Nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012928
Physical Location & Seq
Forward (+)
1825163 .. 1827520
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G038900.1

Sequence Viewer

Length: 939 bp
ATGGACAGGTATAGATGCTTTGGGGGATTTTCCCTTCATGGGTTTGTTTTGTTGCTAGCGTTCGTTTGCGTTATAGTTCCAAGAGCTCAAGGATGGAGCAAAGAGGGTCATATTATGACATGCCGGATTGCACAGGCACTTCTAGAGCCTGAAGCAGCAGAAGCCGTTAGAAATTTACTACCTCACGACGTTGATGGCGACTTGTCCGCTCTCTGCGTATGGCCAGACCAAATCAGGCATTGGTACAGATACCGGTGGACTAGCCCACTTCACTTCATTGACACACCTGATAATGCTTGCAACTTTGACTACTCAAGGGACTGTCATGATACACATGGCTTGAAGAACATGTGTGTTGCTGGTGCCATCCAAAATTTCACCTCTCAGCTTTCACACTACACAGAAGGAACCTCTGATCGTCGATATAATATGACTGAGGCCTTGCTTTTCTTGTCACACTTCATGGGAGATATCCATCAGCCAATGCACGTTGGATTCACGACAGATGAGGGAGGAAACACAATAGACTTGCGTTGGTTCAGACACAAATCAAATCTCCACCATGTATGGGATAGGGAGATTCTACTGCAAGCCCTGAAGGACTATTATGACAAGGACATGGAACTCCTCCTACAAGACATACAGGGAAACATCACAGATGGAATCTGGTCTGATGATGTTACATCATGGCAACATTGTGATGATCACCCTTCATGTGTAAACAAGTATGCTACAGAGAGTATAAACATAGCTTGCAAGTGGGGTTACAAGGACGTCGAGAAGGGTGATACTCTAACAGATGATTACTTCCTTCCTCGGCTACCTGTTGTGGAGAAGAGGATTGCTCAAGGTGGAGTCCGCTTAGCTGCCATCCTCAATCTCGTCTTCTCTTCTCAAGAAACACAAGTTGGTGATGAAGCATCACGTTCACCCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1-P1_nuclease

Protein Analysis

313

Amino Acids

35.76

Weight (kDa)

5.43

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11190
fragaria_vesca FvH4_3g04360 FvH4_3g04360
malus_domestica MD05G1323600.v1.1 MD10G1079400.v1.1 MD10G1301500.v1.1
prunus_persica Prupe.4G038900_v2.0.a1
pyrus_communis pycom10g25420
rosa_chinensis RchiOBHm_Chr5g0006751
rosa_laevigata RLG00000031425
rosa_multiflora Rmu_sc0001044.1_g000042
rosa_roxburghii Rroxscaffold_1G00069290
rosa_rugosa Rorug04G0424200
rosa_samantha Rh5AG052100 Rh5BG051000 Rh5CG059600 Rh5DG050100
rosa_wichuraiana Rw5G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 777
AccB1I GGYRCC 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 209
AciI CCGC 2 cut(s) 207, 859
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 2 cut(s) 172, 373
AcuI CTGAAG 2 cut(s) 171, 617
AcyI GRCGYC 1 cut(s) 774
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 39, 568
AflIII ACRYGT 1 cut(s) 348
AgeI ACCGGT 1 cut(s) 252
AgsI TTSAA 1 cut(s) 343
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
AluBI AGCT 4 cut(s) 86, 388, 752, 866
AluI AGCT 4 cut(s) 86, 388, 752, 866
Alw21I GWGCWC 1 cut(s) 88
AoxI GGCC 2 cut(s) 221, 438
ApeKI GCWGC 2 cut(s) 155, 866
ApoI RAATTY 2 cut(s) 172, 373
AsiGI ACCGGT 1 cut(s) 252
AsuHPI GGTGA 5 cut(s) 370, 698, 797, 921, 923
AsuNHI GCTAGC 1 cut(s) 55
BalI TGGCCA 1 cut(s) 223
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 88
BbsI GAAGAC 1 cut(s) 877
Bbv12I GWGCWC 1 cut(s) 88
BbvI GCAGC 2 cut(s) 167, 853
BccI CCATC 6 cut(s) 87, 188, 374, 483, 653, 878
BceAI ACGGC 1 cut(s) 149
BclI TGATCA 1 cut(s) 703
BfaI CTAG 3 cut(s) 56, 143, 261
BfmI CTRYAG 1 cut(s) 732
BisI GCNGC 2 cut(s) 156, 867
BlpI GCTNAGC 1 cut(s) 862
BlsI GCNGC 2 cut(s) 157, 868
BmiI GGNNCC 2 cut(s) 364, 409
BmsI GCATC 2 cut(s) 5, 929
BmtI GCTAGC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 877
BplI GAGNNNNNCTC 2 cut(s) 829, 861
Bpu1102I GCTNAGC 1 cut(s) 862
BpuEI CTTGAG 4 cut(s) 72, 298, 831, 879
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 774
BsaJI CCNNGG 1 cut(s) 815
BsaWI WCCGGW 1 cut(s) 252
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 568
Bse118I RCCGGY 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 815
BseGI GGATG 3 cut(s) 98, 366, 870
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 39, 568
BseMII CTCAG 2 cut(s) 398, 426
BseRI GAGGAG 1 cut(s) 617
BseXI GCAGC 2 cut(s) 167, 853
BshFI GGCC 2 cut(s) 223, 440
BshNI GGYRCC 1 cut(s) 362
BshTI ACCGGT 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 88
BsiSI CCGG 2 cut(s) 124, 253
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 39, 568
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 2 cut(s) 223, 440
Bsp1286I GDGCHC 1 cut(s) 88
Bsp143I GATC 2 cut(s) 415, 703
Bsp1720I GCTNAGC 1 cut(s) 862
BspACI CCGC 2 cut(s) 207, 859
BspANI GGCC 2 cut(s) 223, 440
BspCNI CTCAG 2 cut(s) 397, 427
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 2 cut(s) 364, 409
BspOI GCTAGC 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 209
BsrFI RCCGGY 1 cut(s) 252
BssAI RCCGGY 1 cut(s) 252
BssECI CCNNGG 1 cut(s) 815
BssMI GATC 2 cut(s) 415, 703
BssNI GRCGYC 1 cut(s) 774
Bst4CI ACNGT 1 cut(s) 323
Bst6I CTCTTC 2 cut(s) 830, 895
BstACI GRCGYC 1 cut(s) 774
BstC8I GCNNGC 4 cut(s) 57, 298, 591, 754
BstDEI CTNAG 3 cut(s) 384, 435, 862
BstF5I GGATG 3 cut(s) 98, 366, 870
BstKTI GATC 2 cut(s) 418, 706
BstMBI GATC 2 cut(s) 415, 703
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNSI RCATGY 2 cut(s) 123, 352
BstSFI CTRYAG 1 cut(s) 732
BstV1I GCAGC 2 cut(s) 167, 853
BstV2I GAAGAC 1 cut(s) 877
BsuRI GGCC 2 cut(s) 223, 440
BtsCI GGATG 3 cut(s) 98, 366, 870
Cac8I GCNNGC 4 cut(s) 57, 298, 591, 754
CciI TCATGA 1 cut(s) 325
Cfr10I RCCGGY 1 cut(s) 252
Csp6I GTAC 1 cut(s) 244
CspAI ACCGGT 1 cut(s) 252
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 3 cut(s) 384, 435, 862
DpnI GATC 2 cut(s) 417, 705
DpnII GATC 2 cut(s) 415, 703
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 830, 895
EarI CTCTTC 2 cut(s) 830, 895
Ecl136II GAGCTC 1 cut(s) 86
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 88
Eco32I GATATC 1 cut(s) 472
Eco53kI GAGCTC 1 cut(s) 86
Eco57I CTGAAG 2 cut(s) 171, 617
EcoICRI GAGCTC 1 cut(s) 86
EcoRV GATATC 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 88
FaqI GGGAC 1 cut(s) 332
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 2 cut(s) 156, 867
FokI GGATG 3 cut(s) 105, 353, 857
FriOI GRGCYC 1 cut(s) 88
Fsp4HI GCNGC 2 cut(s) 156, 867
FspBI CTAG 3 cut(s) 56, 143, 261
GluI GCNGC 2 cut(s) 156, 867
HaeIII GGCC 2 cut(s) 223, 440
HapII CCGG 2 cut(s) 124, 253
Hin1I GRCGYC 1 cut(s) 774
HinfI GANTC 4 cut(s) 495, 580, 663, 855
HpaII CCGG 2 cut(s) 124, 253
HphI GGTGA 5 cut(s) 370, 698, 797, 921, 923
Hpy166II GTNNAC 3 cut(s) 258, 721, 929
Hpy188I TCNGA 3 cut(s) 415, 542, 673
Hpy188III TCNNGA 6 cut(s) 143, 185, 326, 499, 778, 896
Hpy8I GTNNAC 3 cut(s) 258, 721, 929
Hpy99I CGWCG 3 cut(s) 191, 423, 779
HpyAV CCTTC 6 cut(s) 44, 398, 592, 720, 775, 821
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 4 cut(s) 189, 489, 774, 925
HpyCH4V TGCA 5 cut(s) 131, 300, 487, 589, 756
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 3 cut(s) 384, 435, 862
HpySE526I ACGT 4 cut(s) 189, 489, 774, 925
Hsp92I GRCGYC 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 2 cut(s) 415, 703
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 2 cut(s) 167, 853
LweI GCATC 2 cut(s) 5, 929
MaeI CTAG 3 cut(s) 56, 143, 261
MaeII ACGT 4 cut(s) 189, 489, 774, 925
MaeIII GTNAC 3 cut(s) 453, 679, 764
MalI GATC 2 cut(s) 417, 705
MbiI CCGCTC 1 cut(s) 209
MboI GATC 2 cut(s) 415, 703
MboII GAAGA 4 cut(s) 355, 847, 877, 882
MhlI GDGCHC 1 cut(s) 88
MlsI TGGCCA 1 cut(s) 223
MluCI AATT 2 cut(s) 172, 373
MluNI TGGCCA 1 cut(s) 223
MlyI GAGTC 1 cut(s) 864
MmeI TCCRAC 1 cut(s) 472
Mox20I TGGCCA 1 cut(s) 223
MscI TGGCCA 1 cut(s) 223
MslI CAYNNNNRTG 1 cut(s) 699
Msp20I TGGCCA 1 cut(s) 223
MspI CCGG 2 cut(s) 124, 253
MwoI GCNNNNNNNGC 1 cut(s) 161
NdeII GATC 2 cut(s) 415, 703
NheI GCTAGC 1 cut(s) 55
NlaIV GGNNCC 2 cut(s) 364, 409
NmeAIII GCCGAG 1 cut(s) 796
NmuCI GTSAC 1 cut(s) 453
NspI RCATGY 2 cut(s) 123, 352
PagI TCATGA 1 cut(s) 325
PceI AGGCCT 1 cut(s) 440
PciI ACATGT 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 3 cut(s) 495, 580, 663
PinAI ACCGGT 1 cut(s) 252
PkrI GCNGC 2 cut(s) 157, 868
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
PscI ACATGT 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 88
PspN4I GGNNCC 2 cut(s) 364, 409
PsrI GAACNNNNNNTAC 2 cut(s) 615, 647
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 699
SacI GAGCTC 1 cut(s) 88
SatI GCNGC 2 cut(s) 156, 867
Sau3AI GATC 2 cut(s) 415, 703
SchI GAGTC 1 cut(s) 864
SduI GDGCHC 1 cut(s) 88
SfaNI GCATC 2 cut(s) 5, 929
SfcI CTRYAG 1 cut(s) 732
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 4 cut(s) 87, 313, 846, 894
SmoI CTYRAG 4 cut(s) 87, 313, 846, 894
Sse9I AATT 2 cut(s) 172, 373
SseBI AGGCCT 1 cut(s) 440
SsiI CCGC 2 cut(s) 207, 859
SspMI CTAG 3 cut(s) 56, 143, 261
SstI GAGCTC 1 cut(s) 88
StuI AGGCCT 1 cut(s) 440
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 4 cut(s) 192, 492, 777, 928
TaqI TCGA 2 cut(s) 421, 777
TasI AATT 2 cut(s) 172, 373
TfiI GAWTC 3 cut(s) 495, 580, 663
TseFI GTSAC 1 cut(s) 453
TseI GCWGC 2 cut(s) 155, 866
Tsp45I GTSAC 1 cut(s) 453
TspDTI ATGAA 5 cut(s) 26, 265, 451, 702, 930
XapI RAATTY 2 cut(s) 172, 373
XbaI TCTAGA 1 cut(s) 142
XceI RCATGY 2 cut(s) 123, 352
XspI CTAG 3 cut(s) 56, 143, 261
ZraI GACGTC 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.