MD05G1323600.v1.1

S1/P1 Nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012928
Physical Location & Seq
Reverse (-)
45081369 .. 45084050
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1323600.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGAGAAGTATAGATGCTTTGGGGGATTTTCCCTTCATTGGTTTGTTTTGTTGTTGGTTTTTGTTTGCATTATTATGGTTCCAAGGGCTCAAGGATGGAGCAAAGAGGGTCACATGATGATGTGCCGGATTGCGCAGGCACTCCTAGAGCCTGAGGCTGCAGAAGCTGTTCAAAATCTACTACCTCACTACGTGGATGGCGACTTGTCAGCTCTCTGCGTATGGCCGGATCAGATCAGGCATTGGTACAGATACCGGTGGACTAGCCCACTTCACTTCATCGACACACCAGATAATGCTTGCACCTTTGACTACTCAAGGGATTGTCATGATCAACATGGCGTGAAGAACATGTGTGTTGCTGGTGCCGTCCAAAACTTCACCTCTCAACTTTCACACTACACAGAAGGAACATCTGACCGTCTGTGTAAGAAACCCCGATTTTTTTTTATGCATAGATTGATTCCAATGCATGTCGGGTTCACGACAGATGAGGGAGGAAACACGATAGCCTTGCGTTGGTACAGGCACAAATCCAATCTTCACCATGTGTGGGATAGGGAGATCATACAACAAGCTATGAAGGACTATTATGACAAAGACATGGAACTCCTCCTACAAGACATACTGGTGAACATCACAGATGGAATCTGGTCTGATGATGTTACATCATGGAAACACTGCGATGATCAACGTTCATGTATAAACAAGTATGCGACGGAGAGTATAAACATAGCTTGCAAGTGGGCTTACAAGGGCGTTGAGGCGGGTGATACTCTTACAGAGGATTACTTCCTTCCTCGGCTACCTGTGGTGGAGAAGAGGATTGCTCAAGGAGGGGTTCGCTTGGCGGCCATCCTCAACCGCGTCTTCTCTTCCAGTCAAGATACAGGAGTTGATGACGACGTATCACATTCACCCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1-P1_nuclease

Protein Analysis

309

Amino Acids

35.62

Weight (kDa)

5.99

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 33 - 291 3.6e-75 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11190
fragaria_vesca FvH4_3g04360 FvH4_3g04360
malus_domestica MD05G1323600.v1.1 MD10G1079400.v1.1 MD10G1301500.v1.1
prunus_persica Prupe.4G038900_v2.0.a1
pyrus_communis pycom10g25420
rosa_chinensis RchiOBHm_Chr5g0006751
rosa_laevigata RLG00000031425
rosa_multiflora Rmu_sc0001044.1_g000042
rosa_roxburghii Rroxscaffold_1G00069290
rosa_rugosa Rorug04G0424200
rosa_samantha Rh5AG052100 Rh5BG051000 Rh5CG059600 Rh5DG050100
rosa_wichuraiana Rw5G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 365
AccII CGCG 1 cut(s) 867
AciI CCGC 3 cut(s) 767, 851, 865
AclI AACGTT 1 cut(s) 694
AclWI GGATC 1 cut(s) 237
AcoI YGGCCR 2 cut(s) 224, 852
AdeI CACNNNGTG 2 cut(s) 193, 550
AfaI GTAC 2 cut(s) 248, 524
AfiI CCNNNNNNNGG 3 cut(s) 39, 519, 553
AflIII ACRYGT 1 cut(s) 351
AgeI ACCGGT 1 cut(s) 255
AgsI TTSAA 1 cut(s) 173
AluBI AGCT 4 cut(s) 167, 212, 578, 737
AluI AGCT 4 cut(s) 167, 212, 578, 737
AlwI GGATC 1 cut(s) 237
AlwNI CAGNNNCTG 1 cut(s) 167
AoxI GGCC 2 cut(s) 224, 852
ApeKI GCWGC 1 cut(s) 158
AsiGI ACCGGT 1 cut(s) 255
Asp700I GAANNNNTTC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 136
AsuHPI GGTGA 5 cut(s) 373, 536, 643, 782, 909
AxyI CCTNAGG 1 cut(s) 153
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BanI GGYRCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 862
BbvI GCAGC 1 cut(s) 145
BccI CCATC 4 cut(s) 90, 191, 638, 863
BceAI ACGGC 1 cut(s) 353
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BclI TGATCA 2 cut(s) 331, 688
BfaI CTAG 2 cut(s) 146, 264
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 2 cut(s) 159, 852
BlsI GCNGC 2 cut(s) 160, 853
BmiI GGNNCC 2 cut(s) 81, 367
BmsI GCATC 1 cut(s) 5
BpiI GAAGAC 1 cut(s) 862
BplI GAGNNNNNCTC 2 cut(s) 814, 846
BpuEI CTTGAG 3 cut(s) 75, 301, 816
BsaAI YACGTR 1 cut(s) 193
BsaJI CCNNGG 2 cut(s) 83, 800
BsaWI WCCGGW 1 cut(s) 255
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 519, 553
Bse118I RCCGGY 1 cut(s) 255
Bse1I ACTGG 2 cut(s) 633, 879
Bse21I CCTNAGG 1 cut(s) 153
BseDI CCNNGG 2 cut(s) 83, 800
BseGI GGATG 3 cut(s) 101, 202, 855
BseLI CCNNNNNNNGG 3 cut(s) 39, 519, 553
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 2 cut(s) 633, 879
BseRI GAGGAG 1 cut(s) 602
BseXI GCAGC 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 867
BshFI GGCC 2 cut(s) 226, 854
BshNI GGYRCC 1 cut(s) 365
BshTI ACCGGT 1 cut(s) 255
BsiSI CCGG 3 cut(s) 127, 227, 256
BslI CCNNNNNNNGG 3 cut(s) 39, 519, 553
BsnI GGCC 2 cut(s) 226, 854
Bsp1286I GDGCHC 1 cut(s) 91
Bsp143I GATC 5 cut(s) 229, 234, 331, 564, 688
BspACI CCGC 3 cut(s) 767, 851, 865
BspANI GGCC 2 cut(s) 226, 854
BspCNI CTCAG 1 cut(s) 145
BspFNI CGCG 1 cut(s) 867
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 81, 367
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 365
BsrFI RCCGGY 1 cut(s) 255
BsrI ACTGG 2 cut(s) 633, 879
BssAI RCCGGY 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 83, 800
BssMI GATC 5 cut(s) 229, 234, 331, 564, 688
BssT1I CCWWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 422
Bst6I CTCTTC 2 cut(s) 815, 880
BstBAI YACGTR 1 cut(s) 193
BstC8I GCNNGC 3 cut(s) 138, 301, 739
BstDEI CTNAG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 101, 202, 855
BstFNI CGCG 1 cut(s) 867
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 5 cut(s) 232, 237, 334, 567, 691
BstMBI GATC 5 cut(s) 229, 234, 331, 564, 688
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNSI RCATGY 2 cut(s) 355, 476
BstSFI CTRYAG 1 cut(s) 159
BstUI CGCG 1 cut(s) 867
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 1 cut(s) 862
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 2 cut(s) 226, 854
BtgZI GCGATG 1 cut(s) 699
BtsCI GGATG 3 cut(s) 101, 202, 855
BtsI GCAGTG 1 cut(s) 679
BtsIMutI CAGTG 1 cut(s) 679
Cac8I GCNNGC 3 cut(s) 138, 301, 739
CaiI CAGNNNCTG 1 cut(s) 167
CciI TCATGA 1 cut(s) 328
CfoI GCGC 1 cut(s) 136
Cfr10I RCCGGY 1 cut(s) 255
CseI GACGC 1 cut(s) 856
Csp6I GTAC 2 cut(s) 247, 523
CspAI ACCGGT 1 cut(s) 255
CviAII CATG 9 cut(s) 115, 329, 338, 352, 473, 548, 604, 672, 699
CviQI GTAC 2 cut(s) 247, 523
DdeI CTNAG 1 cut(s) 153
DpnI GATC 5 cut(s) 231, 236, 333, 566, 690
DpnII GATC 5 cut(s) 229, 234, 331, 564, 688
DraIII CACNNNGTG 2 cut(s) 193, 550
EaeI YGGCCR 2 cut(s) 224, 852
Eam1104I CTCTTC 2 cut(s) 815, 880
EarI CTCTTC 2 cut(s) 815, 880
Eco130I CCWWGG 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 91
Eco81I CCTNAGG 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 83
EcoT22I ATGCAT 2 cut(s) 456, 474
EcoT38I GRGCYC 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 83
FaeI CATG 9 cut(s) 118, 332, 341, 355, 476, 551, 607, 675, 702
FatI CATG 9 cut(s) 114, 328, 337, 351, 472, 547, 603, 671, 698
FauI CCCGC 1 cut(s) 760
FbaI TGATCA 2 cut(s) 331, 688
Fnu4HI GCNGC 2 cut(s) 159, 852
FokI GGATG 3 cut(s) 108, 209, 842
FriOI GRGCYC 1 cut(s) 91
Fsp4HI GCNGC 2 cut(s) 159, 852
FspBI CTAG 2 cut(s) 146, 264
FspI TGCGCA 1 cut(s) 135
GlaI GCGC 1 cut(s) 135
GluI GCNGC 2 cut(s) 159, 852
HaeIII GGCC 2 cut(s) 226, 854
HapII CCGG 3 cut(s) 127, 227, 256
HgaI GACGC 1 cut(s) 856
HhaI GCGC 1 cut(s) 136
Hin1II CATG 9 cut(s) 118, 332, 341, 355, 476, 551, 607, 675, 702
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HinfI GANTC 2 cut(s) 463, 648
HpaII CCGG 3 cut(s) 127, 227, 256
HphI GGTGA 5 cut(s) 373, 536, 643, 782, 909
Hpy166II GTNNAC 3 cut(s) 261, 483, 634
Hpy188I TCNGA 3 cut(s) 234, 418, 658
Hpy188III TCNNGA 3 cut(s) 329, 484, 884
Hpy8I GTNNAC 3 cut(s) 261, 483, 634
Hpy99I CGWCG 2 cut(s) 721, 908
HpyAV CCTTC 4 cut(s) 44, 401, 577, 806
HpyCH4III ACNGT 1 cut(s) 422
HpyCH4IV ACGT 3 cut(s) 192, 694, 906
HpyCH4V TGCA 6 cut(s) 69, 161, 303, 454, 472, 741
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 3 cut(s) 192, 694, 906
Hsp92II CATG 9 cut(s) 118, 332, 341, 355, 476, 551, 607, 675, 702
HspAI GCGC 1 cut(s) 134
Ksp22I TGATCA 2 cut(s) 331, 688
Kzo9I GATC 5 cut(s) 229, 234, 331, 564, 688
LmnI GCTCC 1 cut(s) 99
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 1 cut(s) 5
MaeI CTAG 2 cut(s) 146, 264
MaeII ACGT 3 cut(s) 192, 694, 906
MaeIII GTNAC 2 cut(s) 110, 664
MalI GATC 5 cut(s) 231, 236, 333, 566, 690
MboI GATC 5 cut(s) 229, 234, 331, 564, 688
MboII GAAGA 5 cut(s) 358, 533, 832, 862, 867
MhlI GDGCHC 1 cut(s) 91
Mph1103I ATGCAT 2 cut(s) 456, 474
MroXI GAANNNNTTC 1 cut(s) 168
MslI CAYNNNNRTG 4 cut(s) 74, 119, 629, 684
MspI CCGG 3 cut(s) 127, 227, 256
MvnI CGCG 1 cut(s) 867
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 5 cut(s) 229, 234, 331, 564, 688
NlaIII CATG 9 cut(s) 118, 332, 341, 355, 476, 551, 607, 675, 702
NlaIV GGNNCC 2 cut(s) 81, 367
NmeAIII GCCGAG 1 cut(s) 781
NmuCI GTSAC 1 cut(s) 110
NsbI TGCGCA 1 cut(s) 135
NsiI ATGCAT 2 cut(s) 456, 474
NspI RCATGY 2 cut(s) 355, 476
PagI TCATGA 1 cut(s) 328
PciI ACATGT 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 168
PfeI GAWTC 2 cut(s) 463, 648
PinAI ACCGGT 1 cut(s) 255
PkrI GCNGC 2 cut(s) 160, 853
Ppu21I YACGTR 1 cut(s) 193
PscI ACATGT 1 cut(s) 351
Psp1406I AACGTT 1 cut(s) 694
PspN4I GGNNCC 2 cut(s) 81, 367
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 167
RsaI GTAC 2 cut(s) 248, 524
RsaNI GTAC 2 cut(s) 247, 523
RseI CAYNNNNRTG 4 cut(s) 74, 119, 629, 684
SatI GCNGC 2 cut(s) 159, 852
Sau3AI GATC 5 cut(s) 229, 234, 331, 564, 688
SduI GDGCHC 1 cut(s) 91
SfaNI GCATC 1 cut(s) 5
SfcI CTRYAG 1 cut(s) 159
SmiMI CAYNNNNRTG 4 cut(s) 74, 119, 629, 684
SmlI CTYRAG 3 cut(s) 90, 316, 831
SmoI CTYRAG 3 cut(s) 90, 316, 831
SsiI CCGC 3 cut(s) 767, 851, 865
SspMI CTAG 2 cut(s) 146, 264
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 1 cut(s) 422
TaiI ACGT 3 cut(s) 195, 697, 909
TaqI TCGA 1 cut(s) 282
TauI GCSGC 1 cut(s) 854
TfiI GAWTC 2 cut(s) 463, 648
TscAI CASTG 1 cut(s) 686
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 4 cut(s) 26, 268, 596, 687
TspGWI ACGGA 1 cut(s) 734
TspRI CASTG 1 cut(s) 686
XceI RCATGY 2 cut(s) 355, 476
XmnI GAANNNNTTC 1 cut(s) 168
XspI CTAG 2 cut(s) 146, 264
Zsp2I ATGCAT 2 cut(s) 456, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.