MD10G1079400.v1.1

S1/P1 Nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012928
Physical Location & Seq
Forward (+)
11454668 .. 11456970
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1079400.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGAGACGTACAGATACTTTGGGAGATTGTCCTTCCATTGGTTTGTTTTGTTGGCTTTTGTTTGCATTTTAGTTCCAAGAGCTCAAGGATGGAGCAAAGAGGGTCATATTATGACGTGCCGGATTGCGCAGGCACTTCTAAAGCCCGAGGCCGCAGAAGCTGTTCGAAATTTACTACCTCACAACGTTGATGGTGATTTATCAGCTCTCTGCGTATGGCCGGATCAGGTCAGGCATTGGTACAGATACAGATGGACTAGCCCGCTTCACTTCATCGATACACCGGATAATGCTTGCACCTTTGACTACTCAAGGGATTGTCATGATCAACATGGCGTGAAGAACATGTGTGTTGCTGGTGCCGTCCAAAACTTCACCTCTCAGCTTTCACACTACACAGAAGGAACATCTGACCGTCGATATAATATGACTGAGGCCTTGCTTTTCTTGTCACACTTTATGGGAGATATCCATCAGCCAATGCATGTCGGGTTCACGACAGATGAGGGAGGAAACACGATAGACTTGCGTTGGTACAAGCACAAATCCAATCTTCACCATGTATGGGATAGGGAGATTATACTACAAGCTATGAAGGACTACTATGACAAAGACATGGAACTCCTCCTACAAGACATACAGGGGAACATCACAGATGGAATCTGGTCTGATGATGTTACATCATGGAAACATTGCGATGATCAACATTCATGTATAAACAAGTATGCTACGGAGAGTATAAACATAGCTTGCAAGTGGGGTTACAAGGACGTTGGGGAGAATTACTTCCTTTCTCGGCTACCTGTGGTGGAGAAGAGGATTGCTCAAGGCGGGGTTCGCTTGGCGGCCATCCTCAATCGCGTCTTTTCTTCCGGTCAAGAGACAGAAGTTGCTGACGAAGCATCACACTCACCCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1-P1_nuclease

Protein Analysis

307

Amino Acids

35.34

Weight (kDa)

5.9

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 31 - 289 2.8e-83 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11190
fragaria_vesca FvH4_3g04360 FvH4_3g04360
malus_domestica MD05G1323600.v1.1 MD10G1079400.v1.1 MD10G1301500.v1.1
prunus_persica Prupe.4G038900_v2.0.a1
pyrus_communis pycom10g25420
rosa_chinensis RchiOBHm_Chr5g0006751
rosa_laevigata RLG00000031425
rosa_multiflora Rmu_sc0001044.1_g000042
rosa_roxburghii Rroxscaffold_1G00069290
rosa_rugosa Rorug04G0424200
rosa_samantha Rh5AG052100 Rh5BG051000 Rh5CG059600 Rh5DG050100
rosa_wichuraiana Rw5G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 359
AccII CGCG 1 cut(s) 861
AciI CCGC 4 cut(s) 153, 263, 831, 845
AclI AACGTT 1 cut(s) 186
AclWI GGATC 1 cut(s) 231
AcoI YGGCCR 2 cut(s) 218, 846
AcsI RAATTY 1 cut(s) 169
AfaI GTAC 3 cut(s) 11, 242, 536
AfiI CCNNNNNNNGG 2 cut(s) 39, 565
AflIII ACRYGT 1 cut(s) 345
AjiI CACGTC 1 cut(s) 117
AluBI AGCT 6 cut(s) 83, 161, 206, 385, 590, 749
AluI AGCT 6 cut(s) 83, 161, 206, 385, 590, 749
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 1 cut(s) 875
AlwI GGATC 1 cut(s) 231
AlwNI CAGNNNCTG 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 4 cut(s) 150, 218, 435, 846
ApoI RAATTY 1 cut(s) 169
Asp700I GAANNNNTTC 2 cut(s) 162, 785
AspLEI GCGC 1 cut(s) 130
AsuHPI GGTGA 4 cut(s) 206, 367, 548, 903
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 146
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 85
BccI CCATC 6 cut(s) 84, 185, 246, 480, 650, 857
BceAI ACGGC 1 cut(s) 347
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BclI TGATCA 2 cut(s) 325, 700
BcoDI GTCTC 1 cut(s) 875
BfaI CTAG 1 cut(s) 258
BisI GCNGC 2 cut(s) 153, 846
BlsI GCNGC 2 cut(s) 154, 847
BmeT110I CYCGRG 1 cut(s) 146
BmgBI CACGTC 1 cut(s) 117
BmiI GGNNCC 1 cut(s) 361
BmsI GCATC 1 cut(s) 911
BplI GAGNNNNNCTC 2 cut(s) 808, 840
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 3 cut(s) 69, 295, 810
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 471
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 2 cut(s) 283, 872
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 565
Bse3DI GCAATG 1 cut(s) 691
Bse8I GATNNNNATC 1 cut(s) 471
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 2 cut(s) 95, 849
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 39, 565
BseMI GCAATG 1 cut(s) 691
BseMII CTCAG 2 cut(s) 395, 423
BseRI GAGGAG 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 861
BshFI GGCC 4 cut(s) 152, 220, 437, 848
BshNI GGYRCC 1 cut(s) 359
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 85
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 4 cut(s) 121, 221, 284, 873
BslI CCNNNNNNNGG 2 cut(s) 39, 565
BsmAI GTCTC 1 cut(s) 875
BsnI GGCC 4 cut(s) 152, 220, 437, 848
BsoBI CYCGRG 1 cut(s) 146
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 3 cut(s) 223, 325, 700
BspACI CCGC 4 cut(s) 153, 263, 831, 845
BspANI GGCC 4 cut(s) 152, 220, 437, 848
BspCNI CTCAG 2 cut(s) 394, 424
BspDI ATCGAT 1 cut(s) 276
BspFNI CGCG 1 cut(s) 861
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 1 cut(s) 361
BspPI GGATC 1 cut(s) 231
BspT104I TTCGAA 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 691
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 3 cut(s) 223, 325, 700
Bst4CI ACNGT 1 cut(s) 416
Bst6I CTCTTC 1 cut(s) 809
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 4 cut(s) 132, 263, 295, 751
BstDEI CTNAG 2 cut(s) 381, 432
BstF5I GGATG 2 cut(s) 95, 849
BstFNI CGCG 1 cut(s) 861
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 3 cut(s) 226, 328, 703
BstMAI GTCTC 1 cut(s) 875
BstMBI GATC 3 cut(s) 223, 325, 700
BstMWI GCNNNNNNNGC 3 cut(s) 158, 837, 899
BstNSI RCATGY 2 cut(s) 349, 488
BstUI CGCG 1 cut(s) 861
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 4 cut(s) 152, 220, 437, 848
BsuTUI ATCGAT 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 711
BtrI CACGTC 1 cut(s) 117
BtsCI GGATG 2 cut(s) 95, 849
Cac8I GCNNGC 4 cut(s) 132, 263, 295, 751
CaiI CAGNNNCTG 1 cut(s) 161
CciI TCATGA 1 cut(s) 322
CfoI GCGC 1 cut(s) 130
ClaI ATCGAT 1 cut(s) 276
CseI GACGC 1 cut(s) 850
Csp6I GTAC 3 cut(s) 10, 241, 535
CviAII CATG 8 cut(s) 323, 332, 346, 485, 560, 616, 684, 711
CviQI GTAC 3 cut(s) 10, 241, 535
DdeI CTNAG 2 cut(s) 381, 432
DpnI GATC 3 cut(s) 225, 327, 702
DpnII GATC 3 cut(s) 223, 325, 700
EaeI YGGCCR 2 cut(s) 218, 846
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
Ecl136II GAGCTC 1 cut(s) 83
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 85
Eco32I GATATC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 83
EcoRV GATATC 1 cut(s) 469
EcoT22I ATGCAT 1 cut(s) 486
EcoT38I GRGCYC 1 cut(s) 85
FaeI CATG 8 cut(s) 326, 335, 349, 488, 563, 619, 687, 714
FatI CATG 8 cut(s) 322, 331, 345, 484, 559, 615, 683, 710
FauI CCCGC 2 cut(s) 270, 824
FbaI TGATCA 2 cut(s) 325, 700
Fnu4HI GCNGC 2 cut(s) 153, 846
FokI GGATG 2 cut(s) 102, 836
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 2 cut(s) 153, 846
FspBI CTAG 1 cut(s) 258
FspI TGCGCA 1 cut(s) 129
GlaI GCGC 1 cut(s) 129
GluI GCNGC 2 cut(s) 153, 846
HaeIII GGCC 4 cut(s) 152, 220, 437, 848
HapII CCGG 4 cut(s) 121, 221, 284, 873
HgaI GACGC 1 cut(s) 850
HhaI GCGC 1 cut(s) 130
Hin1II CATG 8 cut(s) 326, 335, 349, 488, 563, 619, 687, 714
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 1 cut(s) 660
HpaII CCGG 4 cut(s) 121, 221, 284, 873
HphI GGTGA 4 cut(s) 206, 367, 548, 903
Hpy166II GTNNAC 1 cut(s) 495
Hpy188I TCNGA 2 cut(s) 412, 670
Hpy188III TCNNGA 3 cut(s) 323, 496, 878
Hpy8I GTNNAC 1 cut(s) 495
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 3 cut(s) 43, 395, 589
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4IV ACGT 4 cut(s) 8, 116, 186, 771
HpyCH4V TGCA 4 cut(s) 66, 297, 484, 753
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 837, 899
HpyF3I CTNAG 2 cut(s) 381, 432
HpySE526I ACGT 4 cut(s) 8, 116, 186, 771
Hsp92II CATG 8 cut(s) 326, 335, 349, 488, 563, 619, 687, 714
HspAI GCGC 1 cut(s) 128
Ksp22I TGATCA 2 cut(s) 325, 700
Kzo9I GATC 3 cut(s) 223, 325, 700
LmnI GCTCC 1 cut(s) 93
LweI GCATC 1 cut(s) 911
MaeI CTAG 1 cut(s) 258
MaeII ACGT 4 cut(s) 8, 116, 186, 771
MaeIII GTNAC 3 cut(s) 450, 676, 761
MalI GATC 3 cut(s) 225, 327, 702
MboI GATC 3 cut(s) 223, 325, 700
MboII GAAGA 4 cut(s) 352, 545, 826, 861
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 2 cut(s) 169, 781
Mph1103I ATGCAT 1 cut(s) 486
MroXI GAANNNNTTC 2 cut(s) 162, 785
MslI CAYNNNNRTG 1 cut(s) 696
MspI CCGG 4 cut(s) 121, 221, 284, 873
MvnI CGCG 1 cut(s) 861
MwoI GCNNNNNNNGC 3 cut(s) 158, 837, 899
NdeII GATC 3 cut(s) 223, 325, 700
NlaIII CATG 8 cut(s) 326, 335, 349, 488, 563, 619, 687, 714
NlaIV GGNNCC 1 cut(s) 361
NmeAIII GCCGAG 1 cut(s) 775
NmuCI GTSAC 1 cut(s) 450
NsbI TGCGCA 1 cut(s) 129
NsiI ATGCAT 1 cut(s) 486
NspI RCATGY 2 cut(s) 349, 488
NspV TTCGAA 1 cut(s) 166
PagI TCATGA 1 cut(s) 322
PceI AGGCCT 1 cut(s) 437
PciI ACATGT 1 cut(s) 345
PdmI GAANNNNTTC 2 cut(s) 162, 785
PfeI GAWTC 1 cut(s) 660
PkrI GCNGC 2 cut(s) 154, 847
PscI ACATGT 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 85
Psp1406I AACGTT 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 361
PsrI GAACNNNNNNTAC 2 cut(s) 612, 644
PstNI CAGNNNCTG 1 cut(s) 161
RsaI GTAC 3 cut(s) 11, 242, 536
RsaNI GTAC 3 cut(s) 10, 241, 535
RseI CAYNNNNRTG 1 cut(s) 696
SacI GAGCTC 1 cut(s) 85
SatI GCNGC 2 cut(s) 153, 846
Sau3AI GATC 3 cut(s) 223, 325, 700
SduI GDGCHC 1 cut(s) 85
SfaNI GCATC 1 cut(s) 911
SfuI TTCGAA 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 3 cut(s) 84, 310, 825
SmoI CTYRAG 3 cut(s) 84, 310, 825
Sse9I AATT 2 cut(s) 169, 781
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 4 cut(s) 153, 263, 831, 845
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 85
StuI AGGCCT 1 cut(s) 437
TaaI ACNGT 1 cut(s) 416
TaiI ACGT 4 cut(s) 11, 119, 189, 774
TaqI TCGA 3 cut(s) 166, 276, 418
TasI AATT 2 cut(s) 169, 781
TauI GCSGC 2 cut(s) 155, 848
TfiI GAWTC 1 cut(s) 660
TseFI GTSAC 1 cut(s) 450
Tsp45I GTSAC 1 cut(s) 450
TspDTI ATGAA 3 cut(s) 262, 608, 699
TspGWI ACGGA 1 cut(s) 746
XapI RAATTY 1 cut(s) 169
XceI RCATGY 2 cut(s) 349, 488
XmnI GAANNNNTTC 2 cut(s) 162, 785
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.