Rw5G005240

S1/P1 Nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012928
Physical Location & Seq
Reverse (-)
5497044 .. 5499060
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005240.1

Sequence Viewer

Length: 963 bp
ATGGATAGTTACAGAGGCTTTGGAAGATTTGCCAATTATTTTAGTGAATTTGTTTTGTTGCTTGCATTTGTTTGCATTTTAGTCCCGAGAGCTCAGGGATGGAGCAAAGAGGGTCACACTATGACGTGCCGGATTGCACAGGCACTCCTAGAGCCTGAAGCTGCAGAAGCTGTTAAACATTTACTACCTCATGATGTCAATGGCGATCTGTCGGCTCTGTGTGTGTGGCCTGACCAGATCAGGCACTGGTACAGATACCGGTGGACTAGCCCTCTTCACTTCATTGACACACCTGATAATGCTTGCAACTTTGAATACTCAAGAGATTGCCATGATGAACATGGTTCCAAGGACATGTGTGTTGCTGGTGCAGTCCAGAATTTCACATCTCAGCTTTCACACTACACAGAAGGAACATCCGACCGTCGATATAATATGACCGAGGCCTTGCTTTTCTTGTCACATTTTATAGGTGATCTCCATCAGCCAATGCATATTGGATTCACGACAGATAGAGGAGGAAACACAATAGACTTGCGTTGGTACAGGCACAAATCCAATCTTCACCATGTTTGGGATCGAGAGATTTTACTTCAAGCTCTGCATGATTATTATGACAAGGACATGGAACTCCTCCTGCAAGACATACAGGGAAACATCACAGATGGAGTCTGGTCTGATGATGTTACCTCATGGGGACATTGTGATGATATCAGTTCTTGTATAAACAAGTATGCTACAGAAAGTATAAACATAGCTTGCAAATGGGGTTACAAGGGTGTTGAGTCCGGTGATACTTTAACAGAGGATTACTTCCTCCCTCGGCTACCTATTGTGGAGAAGAGGATTGCCCAAGGTGGAGTCCGGTTGGCAGCTATCCTCAATCGTGCCTTCTCCTCTGAATCTGAAGCACAACCTGCTGATCATCACAAAAACAAAAGAGCATCACATTCACCCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1-P1_nuclease

Protein Analysis

320

Amino Acids

36.67

Weight (kDa)

5.77

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 34 - 298 5.2e-87 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11190
fragaria_vesca FvH4_3g04360 FvH4_3g04360
malus_domestica MD05G1323600.v1.1 MD10G1079400.v1.1 MD10G1301500.v1.1
prunus_persica Prupe.4G038900_v2.0.a1
pyrus_communis pycom10g25420
rosa_chinensis RchiOBHm_Chr5g0006751
rosa_laevigata RLG00000031425
rosa_multiflora Rmu_sc0001044.1_g000042
rosa_roxburghii Rroxscaffold_1G00069290
rosa_rugosa Rorug04G0424200
rosa_samantha Rh5AG052100 Rh5BG051000 Rh5CG059600 Rh5DG050100
rosa_wichuraiana Rw5G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 925
AclWI GGATC 1 cut(s) 585
AcsI RAATTY 2 cut(s) 47, 379
AcuI CTGAAG 2 cut(s) 177, 927
AfaI GTAC 2 cut(s) 251, 545
AfiI CCNNNNNNNGG 1 cut(s) 574
AflIII ACRYGT 1 cut(s) 354
AgeI ACCGGT 1 cut(s) 258
AgsI TTSAA 2 cut(s) 314, 596
AjiI CACGTC 1 cut(s) 126
AluBI AGCT 7 cut(s) 92, 161, 170, 394, 599, 758, 875
AluI AGCT 7 cut(s) 92, 161, 170, 394, 599, 758, 875
Alw21I GWGCWC 1 cut(s) 94
AlwI GGATC 1 cut(s) 585
AlwNI CAGNNNCTG 2 cut(s) 170, 246
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 2 cut(s) 227, 444
ApeKI GCWGC 2 cut(s) 161, 872
ApoI RAATTY 2 cut(s) 47, 379
AsiGI ACCGGT 1 cut(s) 258
AsuHPI GGTGA 4 cut(s) 485, 557, 803, 945
AvaI CYCGRG 1 cut(s) 85
BanII GRGCYC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 2 cut(s) 148, 884
BccI CCATC 3 cut(s) 93, 489, 659
BclI TGATCA 1 cut(s) 922
BfaI CTAG 2 cut(s) 149, 267
BfmI CTRYAG 2 cut(s) 162, 738
BfuAI ACCTGC 1 cut(s) 925
BisI GCNGC 2 cut(s) 162, 873
BlsI GCNGC 2 cut(s) 163, 874
BmeT110I CYCGRG 1 cut(s) 85
BmgBI CACGTC 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 346
BmsI GCATC 1 cut(s) 953
Bpu10I CCTNAGC 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 304
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 4 cut(s) 348, 441, 821, 853
BsaWI WCCGGW 3 cut(s) 258, 788, 864
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 1 cut(s) 251
Bse8I GATNNNNATC 1 cut(s) 480
BseDI CCNNGG 4 cut(s) 348, 441, 821, 853
BseGI GGATG 2 cut(s) 104, 416
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 574
BseMII CTCAG 2 cut(s) 107, 404
BseNI ACTGG 1 cut(s) 251
BseRI GAGGAG 3 cut(s) 531, 623, 886
BseXI GCAGC 2 cut(s) 148, 884
BsgI GTGCAG 1 cut(s) 390
Bsh1285I CGRYCG 1 cut(s) 424
BshFI GGCC 2 cut(s) 229, 446
BshTI ACCGGT 1 cut(s) 258
BsiEI CGRYCG 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 4 cut(s) 130, 259, 789, 865
BslFI GGGAC 2 cut(s) 68, 711
BslI CCNNNNNNNGG 1 cut(s) 574
BsmFI GGGAC 2 cut(s) 68, 711
BsnI GGCC 2 cut(s) 229, 446
BsoBI CYCGRG 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 94
Bsp143I GATC 5 cut(s) 205, 237, 475, 577, 922
BspANI GGCC 2 cut(s) 229, 446
BspCNI CTCAG 2 cut(s) 106, 403
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 346
BspMAI CTGCAG 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 925
BspPI GGATC 1 cut(s) 585
BsrFI RCCGGY 1 cut(s) 258
BsrI ACTGG 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 4 cut(s) 348, 441, 821, 853
BssMI GATC 5 cut(s) 205, 237, 475, 577, 922
BssT1I CCWWGG 2 cut(s) 348, 853
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 2 cut(s) 279, 836
BstAPI GCANNNNNTGC 1 cut(s) 917
BstC8I GCNNGC 3 cut(s) 63, 304, 760
BstDEI CTNAG 3 cut(s) 93, 390, 960
BstF5I GGATG 2 cut(s) 104, 416
BstKTI GATC 5 cut(s) 208, 240, 478, 580, 925
BstMBI GATC 5 cut(s) 205, 237, 475, 577, 922
BstMCI CGRYCG 1 cut(s) 424
BstMWI GCNNNNNNNGC 2 cut(s) 167, 917
BstNSI RCATGY 1 cut(s) 358
BstSFI CTRYAG 2 cut(s) 162, 738
BstV1I GCAGC 2 cut(s) 148, 884
BsuRI GGCC 2 cut(s) 229, 446
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 2 cut(s) 104, 416
BtsIMutI CAGTG 1 cut(s) 244
BveI ACCTGC 1 cut(s) 925
Cac8I GCNNGC 3 cut(s) 63, 304, 760
CaiI CAGNNNCTG 2 cut(s) 170, 246
CciI TCATGA 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 258
Csp6I GTAC 2 cut(s) 250, 544
CspAI ACCGGT 1 cut(s) 258
CviAII CATG 8 cut(s) 191, 332, 341, 355, 569, 605, 625, 693
CviQI GTAC 2 cut(s) 250, 544
DdeI CTNAG 3 cut(s) 93, 390, 960
DpnI GATC 5 cut(s) 207, 239, 477, 579, 924
DpnII GATC 5 cut(s) 205, 237, 475, 577, 922
Eam1104I CTCTTC 2 cut(s) 279, 836
EarI CTCTTC 2 cut(s) 279, 836
Ecl136II GAGCTC 1 cut(s) 92
Eco130I CCWWGG 2 cut(s) 348, 853
Eco147I AGGCCT 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 712
Eco53kI GAGCTC 1 cut(s) 92
Eco57I CTGAAG 2 cut(s) 177, 927
Eco88I CYCGRG 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 92
EcoRV GATATC 1 cut(s) 712
EcoT14I CCWWGG 2 cut(s) 348, 853
EcoT22I ATGCAT 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 94
ErhI CCWWGG 2 cut(s) 348, 853
FaeI CATG 8 cut(s) 194, 335, 344, 358, 572, 608, 628, 696
FaqI GGGAC 2 cut(s) 68, 711
FatI CATG 8 cut(s) 190, 331, 340, 354, 568, 604, 624, 692
FbaI TGATCA 1 cut(s) 922
Fnu4HI GCNGC 2 cut(s) 162, 873
FokI GGATG 2 cut(s) 111, 403
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 2 cut(s) 162, 873
FspBI CTAG 2 cut(s) 149, 267
GluI GCNGC 2 cut(s) 162, 873
HaeIII GGCC 2 cut(s) 229, 446
HapII CCGG 4 cut(s) 130, 259, 789, 865
Hin1II CATG 8 cut(s) 194, 335, 344, 358, 572, 608, 628, 696
HinfI GANTC 5 cut(s) 501, 669, 785, 861, 902
HpaII CCGG 4 cut(s) 130, 259, 789, 865
HphI GGTGA 4 cut(s) 485, 557, 803, 945
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 4 cut(s) 421, 679, 901, 907
Hpy188III TCNNGA 6 cut(s) 85, 191, 321, 376, 505, 581
Hpy8I GTNNAC 1 cut(s) 264
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 2 cut(s) 404, 901
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 1 cut(s) 125
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 917
HpyF3I CTNAG 3 cut(s) 93, 390, 960
HpySE526I ACGT 1 cut(s) 125
Hsp92II CATG 8 cut(s) 194, 335, 344, 358, 572, 608, 628, 696
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 5 cut(s) 205, 237, 475, 577, 922
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 2 cut(s) 148, 884
LweI GCATC 1 cut(s) 953
MaeI CTAG 2 cut(s) 149, 267
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 5 cut(s) 8, 113, 459, 685, 770
MalI GATC 5 cut(s) 207, 239, 477, 579, 924
MboI GATC 5 cut(s) 205, 237, 475, 577, 922
MboII GAAGA 4 cut(s) 36, 266, 554, 853
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 3 cut(s) 34, 47, 379
MlyI GAGTC 3 cut(s) 678, 794, 870
MmeI TCCRAC 1 cut(s) 444
Mph1103I ATGCAT 1 cut(s) 495
MseI TTAA 2 cut(s) 174, 800
MslI CAYNNNNRTG 1 cut(s) 705
MspI CCGG 4 cut(s) 130, 259, 789, 865
MwoI GCNNNNNNNGC 2 cut(s) 167, 917
NdeII GATC 5 cut(s) 205, 237, 475, 577, 922
NlaIII CATG 8 cut(s) 194, 335, 344, 358, 572, 608, 628, 696
NlaIV GGNNCC 1 cut(s) 346
NmeAIII GCCGAG 1 cut(s) 802
NmuCI GTSAC 2 cut(s) 113, 459
NsiI ATGCAT 1 cut(s) 495
NspI RCATGY 1 cut(s) 358
PagI TCATGA 1 cut(s) 190
PceI AGGCCT 1 cut(s) 446
PciI ACATGT 1 cut(s) 354
PfeI GAWTC 2 cut(s) 501, 902
PinAI ACCGGT 1 cut(s) 258
PkrI GCNGC 2 cut(s) 163, 874
PleI GAGTC 3 cut(s) 677, 793, 869
PpsI GAGTC 3 cut(s) 677, 793, 869
PscI ACATGT 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 346
PstI CTGCAG 1 cut(s) 166
PstNI CAGNNNCTG 2 cut(s) 170, 246
RsaI GTAC 2 cut(s) 251, 545
RsaNI GTAC 2 cut(s) 250, 544
RseI CAYNNNNRTG 1 cut(s) 705
SacI GAGCTC 1 cut(s) 94
SaqAI TTAA 2 cut(s) 174, 800
SatI GCNGC 2 cut(s) 162, 873
Sau3AI GATC 5 cut(s) 205, 237, 475, 577, 922
SchI GAGTC 3 cut(s) 678, 794, 870
SduI GDGCHC 1 cut(s) 94
SfaNI GCATC 1 cut(s) 953
SfcI CTRYAG 2 cut(s) 162, 738
SmiMI CAYNNNNRTG 1 cut(s) 705
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
Sse9I AATT 3 cut(s) 34, 47, 379
SseBI AGGCCT 1 cut(s) 446
SspMI CTAG 2 cut(s) 149, 267
SstI GAGCTC 1 cut(s) 94
StuI AGGCCT 1 cut(s) 446
StyI CCWWGG 2 cut(s) 348, 853
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 1 cut(s) 128
TaqI TCGA 2 cut(s) 427, 580
TaqII GACCGA 1 cut(s) 455
TasI AATT 3 cut(s) 34, 47, 379
TfiI GAWTC 2 cut(s) 501, 902
Tru1I TTAA 2 cut(s) 174, 800
Tru9I TTAA 2 cut(s) 174, 800
TscAI CASTG 1 cut(s) 251
TseFI GTSAC 2 cut(s) 113, 459
TseI GCWGC 2 cut(s) 161, 872
Tsp45I GTSAC 2 cut(s) 113, 459
TspDTI ATGAA 2 cut(s) 271, 351
TspRI CASTG 1 cut(s) 251
XapI RAATTY 2 cut(s) 47, 379
XceI RCATGY 1 cut(s) 358
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XspI CTAG 2 cut(s) 149, 267
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.