MD10G1301500.v1.1

S1/P1 Nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012928
Physical Location & Seq
Reverse (-)
38858995 .. 38863207
4213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1301500.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGTTTACGTATACGAACGTTTCTCTAATTATATGCTCATGGGATTCTCGAGTATGGAACATATTGGTTTAGCAGCTTGTGCGTGCTGGTGTATATGGTGCAAGTGTGATCACTGGTCAGCAATTCACAAGAGAATTAAGATGGAGACGTACAGATACTTTGGGAGATTGTCCTTCCATTGGTTTGTTTTGTTGGCTTTTGTTTGCATTTTAGTTCCAAGAGCTCAAGGATGGAGCAAAGAGGGTCATATTATGACGTGCCGGATTGCGCAGGCACTTCTAAAGCCCGAGGCCGCAGAAGCTGTTCGAAATTTACTACCTCACAACGTTGATGGTGATTTATCAGCTCTCTGCGTATGGCCGGATCAGGTCAGGCATTGGTACAGATACAGATGGACTAGCCCGCTTCACTTCATCGATACACCGGATAATGCTTGCACCTTTGACTACTCAAGGGATTGTCATGATCAACATGGCGTGAAGAACATGTGTGTTGCTGGTGCCGTCCAAAACTTCACCTCTCAGCTTTCACACTACACAGAAGGAACATCTGACCGTCGATATAATATGACTGAGGCCTTGCTTTTCTTGTCACACTTTATGGGAGATATCCATCAGCCAATGCATGTCGGGTTCACGACAGATGAGGGAGGAAACACGATAGACTTGCGTTGGTACAAGCACAAATCCAATCTTCACCATGTATGGGATAGGGAGATTATACTACAAGCTATGAAGGACTACTATGACAAAGACATGGAACTCCTCCTACAAGACATACAGGGGAACATCACAGATGGAATCTGGTCTGATGATGTTACATCATGGAAACATTGCGATGATCAACATTCATGTATAAACAAGTATGCTACGGAGAGTATAAACATAGCTTGCAAGTGGGGTTACAAGGACGTTGGGGTGGGTGATACTCTTACAGAGAATTACTTCCTTTCTCGGCTACCTGTGGTGGAGAAGAGGATTGCTCAAGGCGGGGTTCGCTTGGCGGCCATCCTCAATCGCGTCTTTTCTTCCGGTCAAGAGACAGAAGTTGCTGACGAAGCATCACACTCACCCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1-P1_nuclease

Protein Analysis

360

Amino Acids

41.53

Weight (kDa)

6.11

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 78 - 342 1.5e-87 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11190
fragaria_vesca FvH4_3g04360 FvH4_3g04360
malus_domestica MD05G1323600.v1.1 MD10G1079400.v1.1 MD10G1301500.v1.1
prunus_persica Prupe.4G038900_v2.0.a1
pyrus_communis pycom10g25420
rosa_chinensis RchiOBHm_Chr5g0006751
rosa_laevigata RLG00000031425
rosa_multiflora Rmu_sc0001044.1_g000042
rosa_roxburghii Rroxscaffold_1G00069290
rosa_rugosa Rorug04G0424200
rosa_samantha Rh5AG052100 Rh5BG051000 Rh5CG059600 Rh5DG050100
rosa_wichuraiana Rw5G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 500
AccI GTMKAC 1 cut(s) 12
AccII CGCG 1 cut(s) 1020
AciI CCGC 4 cut(s) 294, 404, 990, 1004
AclI AACGTT 2 cut(s) 19, 327
AclWI GGATC 1 cut(s) 372
AcoI YGGCCR 2 cut(s) 359, 1005
AcsI RAATTY 1 cut(s) 310
AfaI GTAC 3 cut(s) 152, 383, 677
AfiI CCNNNNNNNGG 2 cut(s) 180, 706
AflIII ACRYGT 1 cut(s) 486
AjiI CACGTC 1 cut(s) 258
AluBI AGCT 7 cut(s) 77, 224, 302, 347, 526, 731, 890
AluI AGCT 7 cut(s) 77, 224, 302, 347, 526, 731, 890
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 2 cut(s) 140, 1034
AlwI GGATC 1 cut(s) 372
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 2 cut(s) 49, 287
AoxI GGCC 4 cut(s) 291, 359, 576, 1005
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 310
Asp700I GAANNNNTTC 2 cut(s) 303, 944
AspLEI GCGC 1 cut(s) 271
AsuHPI GGTGA 5 cut(s) 347, 508, 689, 935, 1062
AsuII TTCGAA 1 cut(s) 307
AvaI CYCGRG 2 cut(s) 49, 287
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 226
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 1 cut(s) 86
BccI CCATC 7 cut(s) 136, 225, 326, 387, 621, 791, 1016
BceAI ACGGC 1 cut(s) 488
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BclI TGATCA 3 cut(s) 109, 466, 841
BcoDI GTCTC 2 cut(s) 140, 1034
BfaI CTAG 1 cut(s) 399
BisI GCNGC 3 cut(s) 75, 294, 1005
BlsI GCNGC 3 cut(s) 76, 295, 1006
BmeT110I CYCGRG 2 cut(s) 49, 287
BmgBI CACGTC 1 cut(s) 258
BmiI GGNNCC 1 cut(s) 502
BmsI GCATC 1 cut(s) 1070
BplI GAGNNNNNCTC 2 cut(s) 967, 999
Bpu14I TTCGAA 1 cut(s) 307
BpuEI CTTGAG 3 cut(s) 210, 436, 969
Bsa29I ATCGAT 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 10
BsaBI GATNNNNATC 1 cut(s) 612
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 2 cut(s) 424, 1031
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 706
Bse1I ACTGG 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 832
Bse8I GATNNNNATC 1 cut(s) 612
BseCI ATCGAT 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 2 cut(s) 236, 1008
BseJI GATNNNNATC 1 cut(s) 612
BseLI CCNNNNNNNGG 2 cut(s) 180, 706
BseMI GCAATG 1 cut(s) 832
BseMII CTCAG 2 cut(s) 536, 564
BseNI ACTGG 1 cut(s) 119
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 1 cut(s) 86
Bsh1236I CGCG 1 cut(s) 1020
BshFI GGCC 4 cut(s) 293, 361, 578, 1007
BshNI GGYRCC 1 cut(s) 500
BshVI ATCGAT 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 226
BsiHKCI CYCGRG 2 cut(s) 49, 287
BsiSI CCGG 4 cut(s) 262, 362, 425, 1032
BslI CCNNNNNNNGG 2 cut(s) 180, 706
BsmAI GTCTC 2 cut(s) 140, 1034
BsmBI CGTCTC 1 cut(s) 140
BsnI GGCC 4 cut(s) 293, 361, 578, 1007
BsoBI CYCGRG 2 cut(s) 49, 287
Bsp119I TTCGAA 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 226
Bsp143I GATC 4 cut(s) 109, 364, 466, 841
BspACI CCGC 4 cut(s) 294, 404, 990, 1004
BspANI GGCC 4 cut(s) 293, 361, 578, 1007
BspCNI CTCAG 2 cut(s) 535, 565
BspDI ATCGAT 1 cut(s) 417
BspFNI CGCG 1 cut(s) 1020
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 502
BspPI GGATC 1 cut(s) 372
BspT104I TTCGAA 1 cut(s) 307
BspT107I GGYRCC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 832
BsrI ACTGG 1 cut(s) 119
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 4 cut(s) 109, 364, 466, 841
BssNAI GTATAC 1 cut(s) 13
Bst1107I GTATAC 1 cut(s) 13
Bst4CI ACNGT 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 968
BstAPI GCANNNNNTGC 1 cut(s) 80
BstBAI YACGTR 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 307
BstC8I GCNNGC 5 cut(s) 85, 273, 404, 436, 892
BstDEI CTNAG 2 cut(s) 522, 573
BstF5I GGATG 2 cut(s) 236, 1008
BstFNI CGCG 1 cut(s) 1020
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 4 cut(s) 112, 367, 469, 844
BstMAI GTCTC 2 cut(s) 140, 1034
BstMBI GATC 4 cut(s) 109, 364, 466, 841
BstMWI GCNNNNNNNGC 4 cut(s) 80, 299, 996, 1058
BstNSI RCATGY 2 cut(s) 490, 629
BstSNI TACGTA 1 cut(s) 10
BstUI CGCG 1 cut(s) 1020
BstV1I GCAGC 1 cut(s) 86
BstZ17I GTATAC 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 417
BsuRI GGCC 4 cut(s) 293, 361, 578, 1007
BsuTUI ATCGAT 1 cut(s) 417
BtgZI GCGATG 1 cut(s) 852
BtrI CACGTC 1 cut(s) 258
BtsCI GGATG 2 cut(s) 236, 1008
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 5 cut(s) 85, 273, 404, 436, 892
CaiI CAGNNNCTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 463
CfoI GCGC 1 cut(s) 271
ClaI ATCGAT 1 cut(s) 417
CseI GACGC 1 cut(s) 1009
Csp6I GTAC 3 cut(s) 151, 382, 676
CviAII CATG 9 cut(s) 40, 464, 473, 487, 626, 701, 757, 825, 852
CviQI GTAC 3 cut(s) 151, 382, 676
DdeI CTNAG 2 cut(s) 522, 573
DpnI GATC 4 cut(s) 111, 366, 468, 843
DpnII GATC 4 cut(s) 109, 364, 466, 841
EaeI YGGCCR 2 cut(s) 359, 1005
Eam1104I CTCTTC 1 cut(s) 968
EarI CTCTTC 1 cut(s) 968
Ecl136II GAGCTC 1 cut(s) 224
Eco105I TACGTA 1 cut(s) 10
Eco147I AGGCCT 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 226
Eco32I GATATC 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 224
Eco88I CYCGRG 2 cut(s) 49, 287
EcoICRI GAGCTC 1 cut(s) 224
EcoRV GATATC 1 cut(s) 610
EcoT22I ATGCAT 1 cut(s) 627
EcoT38I GRGCYC 1 cut(s) 226
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 9 cut(s) 43, 467, 476, 490, 629, 704, 760, 828, 855
FatI CATG 9 cut(s) 39, 463, 472, 486, 625, 700, 756, 824, 851
FauI CCCGC 2 cut(s) 411, 983
FbaI TGATCA 3 cut(s) 109, 466, 841
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 75, 294, 1005
FokI GGATG 2 cut(s) 243, 995
FriOI GRGCYC 1 cut(s) 226
Fsp4HI GCNGC 3 cut(s) 75, 294, 1005
FspBI CTAG 1 cut(s) 399
FspI TGCGCA 1 cut(s) 270
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 75, 294, 1005
HaeIII GGCC 4 cut(s) 293, 361, 578, 1007
HapII CCGG 4 cut(s) 262, 362, 425, 1032
HgaI GACGC 1 cut(s) 1009
HhaI GCGC 1 cut(s) 271
Hin1II CATG 9 cut(s) 43, 467, 476, 490, 629, 704, 760, 828, 855
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 2 cut(s) 45, 801
HpaII CCGG 4 cut(s) 262, 362, 425, 1032
HphI GGTGA 5 cut(s) 347, 508, 689, 935, 1062
Hpy166II GTNNAC 3 cut(s) 7, 13, 636
Hpy188I TCNGA 2 cut(s) 553, 811
Hpy188III TCNNGA 4 cut(s) 49, 464, 637, 1037
Hpy8I GTNNAC 3 cut(s) 7, 13, 636
Hpy99I CGWCG 1 cut(s) 561
HpyAV CCTTC 3 cut(s) 184, 536, 730
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4IV ACGT 6 cut(s) 9, 19, 149, 257, 327, 912
HpyCH4V TGCA 5 cut(s) 102, 207, 438, 625, 894
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 299, 996, 1058
HpyF3I CTNAG 2 cut(s) 522, 573
HpySE526I ACGT 6 cut(s) 9, 19, 149, 257, 327, 912
Hsp92II CATG 9 cut(s) 43, 467, 476, 490, 629, 704, 760, 828, 855
HspAI GCGC 1 cut(s) 269
Ksp22I TGATCA 3 cut(s) 109, 466, 841
Kzo9I GATC 4 cut(s) 109, 364, 466, 841
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 1 cut(s) 1070
MaeI CTAG 1 cut(s) 399
MaeII ACGT 6 cut(s) 9, 19, 149, 257, 327, 912
MaeIII GTNAC 3 cut(s) 591, 817, 902
MalI GATC 4 cut(s) 111, 366, 468, 843
MboI GATC 4 cut(s) 109, 364, 466, 841
MboII GAAGA 4 cut(s) 493, 686, 985, 1020
MhlI GDGCHC 1 cut(s) 226
MluCI AATT 5 cut(s) 28, 123, 135, 310, 940
Mph1103I ATGCAT 1 cut(s) 627
MroXI GAANNNNTTC 2 cut(s) 303, 944
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 837
MspI CCGG 4 cut(s) 262, 362, 425, 1032
MvnI CGCG 1 cut(s) 1020
MwoI GCNNNNNNNGC 4 cut(s) 80, 299, 996, 1058
NdeII GATC 4 cut(s) 109, 364, 466, 841
NlaIII CATG 9 cut(s) 43, 467, 476, 490, 629, 704, 760, 828, 855
NlaIV GGNNCC 1 cut(s) 502
NmeAIII GCCGAG 1 cut(s) 934
NmuCI GTSAC 1 cut(s) 591
NsbI TGCGCA 1 cut(s) 270
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 2 cut(s) 490, 629
NspV TTCGAA 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 49
PagI TCATGA 1 cut(s) 463
PceI AGGCCT 1 cut(s) 578
PciI ACATGT 1 cut(s) 486
PdmI GAANNNNTTC 2 cut(s) 303, 944
PfeI GAWTC 2 cut(s) 45, 801
PkrI GCNGC 3 cut(s) 76, 295, 1006
Ppu21I YACGTR 1 cut(s) 10
PscI ACATGT 1 cut(s) 486
Psp124BI GAGCTC 1 cut(s) 226
Psp1406I AACGTT 2 cut(s) 19, 327
PspN4I GGNNCC 1 cut(s) 502
PsrI GAACNNNNNNTAC 2 cut(s) 753, 785
PstNI CAGNNNCTG 1 cut(s) 302
RsaI GTAC 3 cut(s) 152, 383, 677
RsaNI GTAC 3 cut(s) 151, 382, 676
RseI CAYNNNNRTG 1 cut(s) 837
SacI GAGCTC 1 cut(s) 226
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 3 cut(s) 75, 294, 1005
Sau3AI GATC 4 cut(s) 109, 364, 466, 841
SduI GDGCHC 1 cut(s) 226
SfaNI GCATC 1 cut(s) 1070
Sfr274I CTCGAG 1 cut(s) 49
SfuI TTCGAA 1 cut(s) 307
SlaI CTCGAG 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 837
SmlI CTYRAG 4 cut(s) 49, 225, 451, 984
SmoI CTYRAG 4 cut(s) 49, 225, 451, 984
SnaBI TACGTA 1 cut(s) 10
Sse9I AATT 5 cut(s) 28, 123, 135, 310, 940
SseBI AGGCCT 1 cut(s) 578
SsiI CCGC 4 cut(s) 294, 404, 990, 1004
SspMI CTAG 1 cut(s) 399
SstI GAGCTC 1 cut(s) 226
StuI AGGCCT 1 cut(s) 578
TaaI ACNGT 1 cut(s) 557
TaiI ACGT 6 cut(s) 12, 22, 152, 260, 330, 915
TaqI TCGA 4 cut(s) 50, 307, 417, 559
TasI AATT 5 cut(s) 28, 123, 135, 310, 940
TauI GCSGC 2 cut(s) 296, 1007
TfiI GAWTC 2 cut(s) 45, 801
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 1 cut(s) 591
TseI GCWGC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 591
TspDTI ATGAA 3 cut(s) 403, 749, 840
TspGWI ACGGA 1 cut(s) 887
TspRI CASTG 1 cut(s) 119
XapI RAATTY 1 cut(s) 310
XceI RCATGY 2 cut(s) 490, 629
XhoI CTCGAG 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 12
XmnI GAANNNNTTC 2 cut(s) 303, 944
XspI CTAG 1 cut(s) 399
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.