FvH4_3g05461

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011247
Physical Location & Seq
Forward (+)
3195672 .. 3197935
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05461.t1

Sequence Viewer

Length: 1218 bp
ATGCAACGGGTACGGTTGTCCTCTCAACAGGCGCCTGTGCTCAAGCTAGGGGATTCTCAGATGACATTATCCCCAAAGTTTAGGCTAGCGGTTATCCAATCTTCTTTGATGGACCGTTCTTCAGATATTGAGTTATCACTTAGGGGAGAACCCCTCATTCCTGGTCTACCTGATGATGTTGCGCTTAACTGTCTACTTCGGCTTCCGGTGGAAAGCCACACAGCAAGCAAAGCTGTCTGCAAGCGTTGGCATCTACTGTTTGGTAGTAAAGAGCGTTTTTTCACTCGAAGAAAAGAAGTAGGGTTTAAGGACCCGTGGCTTTTTGTCTTTGCATTCCACAAGTGCACTGGAAAGATTCAGTGGCAGGTTCTTGATCTCACTCATTTCTCGTGGCACACAATCCCCGCCATGCCTTGCAAAGACAAAGTTTGTCCACATGGGTTCAGATGTGTTGCACTTCCCCATGAGGGCACTCTCTTTGTTTGTGGTGGTTTGGTCTCTGATGTGGATTGTCCGCTGGATTTGGTCCTGAAATATGAAATGCAGAAGAATCGATGGACTGTGGTGAACCAGATGGTGAGTGCTAGGTCTTTTTTTGCAACTGGAGTGATTGATGGGATGATTTATGTGGCTGGAGGAAACAGCACAGATCTTTTTGAGCTAGACTCCGCTGAGGTTTTGGATCCTGCTGAAGGGAGTTGGCATCCCATTGCGAACATGGGAACAAATATGTCTTCTTATGATGCGGCAGTTCTCGATGGAAAGCTTCTGGTTACGGAAGGCTGGTTGTGGCCTTTCTATGTATCTCCTAGGGGTCAGGTTTATGATCCAAGAACTGATAATTGGGAGAGTATGGCTGTTGGACTCAGAGAGGGATGGACAGGTTCAAGTGTGGTGATTTATGGTCATTTGTTTGTGGTCTCAGAGCTTGAAAGAATGAAGCTTAAGGTTTATGATGTGGATACTGATTCCTGGGACACAATAGAGGGCCCTCCTTTACCTGAGCAGATATGTAAACCCTTTGCAGTGAATTCTTATGATTGTACAATATGTGTCGTTGGTAGGAACCTTCATCTTGCTGTCGGTAACATCTCAAGGGTTAGCCGTAAAGGCTCTTCTGACAACAAGTGGTACTTCAGTGTTCAATGGCATGTGGTTGATGCCCCAGATAACTTGTCTGACTTGACACCTTCAAGCTCACAGGTCCTCTTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

406

Amino Acids

45.4

Weight (kDa)

6.01

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 54 - 93 8.9e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 122 - 286 6.1e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 161 - 331 2.3e-18 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 162 - 284 6.7e-09 Calicin, beta-propeller domain
Kelch_1 PF01344 195 - 241 1.5e-09 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 355
AccB1I GGYRCC 1 cut(s) 31
AccI GTMKAC 2 cut(s) 166, 193
AciI CCGC 5 cut(s) 89, 405, 515, 669, 746
AclWI GGATC 3 cut(s) 677, 690, 821
AcsI RAATTY 1 cut(s) 1030
AcuI CTGAAG 3 cut(s) 105, 711, 1120
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 3 cut(s) 12, 1045, 1133
AfiI CCNNNNNNNGG 2 cut(s) 467, 692
AflII CTTAAG 1 cut(s) 944
AgsI TTSAA 4 cut(s) 888, 932, 1145, 1194
AjnI CCWGG 2 cut(s) 160, 971
AjuI GAANNNNNNNTTGG 2 cut(s) 826, 858
AluBI AGCT 7 cut(s) 46, 233, 661, 766, 928, 943, 1197
AluI AGCT 7 cut(s) 46, 233, 661, 766, 928, 943, 1197
Alw21I GWGCWC 2 cut(s) 42, 347
Alw26I GTCTC 2 cut(s) 502, 925
Alw44I GTGCAC 1 cut(s) 343
AlwI GGATC 3 cut(s) 677, 690, 821
AoxI GGCC 2 cut(s) 791, 988
ApaI GGGCCC 1 cut(s) 992
ApaLI GTGCAC 1 cut(s) 343
ApoI RAATTY 1 cut(s) 1030
AspA2I CCTAGG 1 cut(s) 809
AspLEI GCGC 2 cut(s) 34, 184
AspS9I GGNCC 6 cut(s) 112, 310, 526, 988, 989, 1204
AsuHPI GGTGA 3 cut(s) 577, 589, 907
AsuNHI GCTAGC 1 cut(s) 85
AvaII GGWCC 4 cut(s) 112, 310, 526, 1204
AvrII CCTAGG 1 cut(s) 809
BaeGI GKGCMC 3 cut(s) 347, 473, 992
BaeI ACNNNNGTAYC 3 cut(s) 35, 1123, 1156
BamHI GGATCC 1 cut(s) 682
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 992
BarI GAAGNNNNNNTAC 2 cut(s) 1099, 1131
BauI CACGAG 1 cut(s) 388
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 2 cut(s) 42, 347
BbvCI CCTCAGC 1 cut(s) 672
BccI CCATC 6 cut(s) 103, 549, 568, 608, 752, 870
BceAI ACGGC 1 cut(s) 1089
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 2 cut(s) 162, 973
BciVI GTATCC 1 cut(s) 955
BcoDI GTCTC 2 cut(s) 502, 925
BfaI CTAG 6 cut(s) 47, 86, 585, 662, 810, 1216
BfoI RGCGCY 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 944
BfuAI ACCTGC 1 cut(s) 355
BfuI GTATCC 1 cut(s) 955
BglI GCCNNNNNGGC 1 cut(s) 1110
BglII AGATCT 1 cut(s) 649
BisI GCNGC 1 cut(s) 747
BlnI CCTAGG 1 cut(s) 809
BlsI GCNGC 1 cut(s) 748
Bme1390I CCNGG 2 cut(s) 162, 973
Bme18I GGWCC 4 cut(s) 112, 310, 526, 1204
BmgT120I GGNCC 6 cut(s) 112, 310, 526, 988, 989, 1204
BmiI GGNNCC 5 cut(s) 33, 312, 684, 990, 1067
BmrFI CCNGG 2 cut(s) 162, 973
BmsI GCATC 4 cut(s) 259, 712, 733, 1150
BmtI GCTAGC 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 726
BplI GAGNNNNNCTC 4 cut(s) 138, 170, 650, 682
BpmI CTGGAG 2 cut(s) 624, 654
Bpu10I CCTNAGC 2 cut(s) 672, 1002
BpuEI CTTGAG 2 cut(s) 26, 1078
Bsa29I ATCGAT 1 cut(s) 553
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 2 cut(s) 502, 925
BsaJI CCNNGG 3 cut(s) 314, 809, 972
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 2 cut(s) 467, 692
Bse1I ACTGG 2 cut(s) 352, 607
Bse3DI GCAATG 1 cut(s) 708
BseBI CCWGG 2 cut(s) 162, 973
BseCI ATCGAT 1 cut(s) 553
BseDI CCNNGG 3 cut(s) 314, 809, 972
BseGI GGATG 3 cut(s) 624, 703, 881
BseLI CCNNNNNNNGG 2 cut(s) 467, 692
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 5 cut(s) 71, 663, 880, 936, 993
BseNI ACTGG 2 cut(s) 352, 607
BseSI GKGCMC 3 cut(s) 347, 473, 992
BshFI GGCC 2 cut(s) 793, 990
BshNI GGYRCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 553
BsiHKAI GWGCWC 2 cut(s) 42, 347
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 989
BslI CCNNNNNNNGG 2 cut(s) 467, 692
BsmAI GTCTC 2 cut(s) 502, 925
BsmFI GGGAC 1 cut(s) 989
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 2 cut(s) 793, 990
Bso31I GGTCTC 2 cut(s) 502, 925
Bsp120I GGGCCC 1 cut(s) 988
Bsp1286I GDGCHC 4 cut(s) 42, 347, 473, 992
Bsp1407I TGTACA 1 cut(s) 1043
Bsp143I GATC 4 cut(s) 373, 649, 682, 826
BspACI CCGC 5 cut(s) 89, 405, 515, 669, 746
BspANI GGCC 2 cut(s) 793, 990
BspCNI CTCAG 5 cut(s) 70, 664, 879, 935, 994
BspDI ATCGAT 1 cut(s) 553
BspLI GGNNCC 5 cut(s) 33, 312, 684, 990, 1067
BspMI ACCTGC 1 cut(s) 355
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 3 cut(s) 677, 690, 821
BspQI GCTCTTC 1 cut(s) 1120
BspT107I GGYRCC 1 cut(s) 31
BspTI CTTAAG 1 cut(s) 944
BspTNI GGTCTC 2 cut(s) 502, 925
BsrDI GCAATG 1 cut(s) 708
BsrGI TGTACA 1 cut(s) 1043
BsrI ACTGG 2 cut(s) 352, 607
BssECI CCNNGG 3 cut(s) 314, 809, 972
BssMI GATC 4 cut(s) 373, 649, 682, 826
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 1 cut(s) 388
BssT1I CCWWGG 1 cut(s) 809
Bst2BI CACGAG 1 cut(s) 388
Bst2UI CCWGG 2 cut(s) 162, 973
Bst4CI ACNGT 5 cut(s) 15, 116, 191, 258, 562
Bst6I CTCTTC 1 cut(s) 1120
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 944
BstAUI TGTACA 1 cut(s) 1043
BstC8I GCNNGC 3 cut(s) 87, 226, 242
BstDEI CTNAG 6 cut(s) 57, 140, 672, 866, 922, 1002
BstDSI CCRYGG 1 cut(s) 314
BstENI CCTNNNNNAGG 1 cut(s) 690
BstF5I GGATG 3 cut(s) 624, 703, 881
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 2 cut(s) 34, 184
BstKTI GATC 4 cut(s) 376, 652, 685, 829
BstMAI GTCTC 2 cut(s) 502, 925
BstMBI GATC 4 cut(s) 373, 649, 682, 826
BstMWI GCNNNNNNNGC 2 cut(s) 230, 1110
BstNI CCWGG 2 cut(s) 162, 973
BstNSI RCATGY 1 cut(s) 1154
BstSCI CCNGG 2 cut(s) 160, 971
BstSLI GKGCMC 3 cut(s) 347, 473, 992
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 2 cut(s) 649, 682
BstYI RGATCY 2 cut(s) 649, 682
Bsu15I ATCGAT 1 cut(s) 553
BsuI GTATCC 1 cut(s) 955
BsuRI GGCC 2 cut(s) 793, 990
BsuTUI ATCGAT 1 cut(s) 553
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 3 cut(s) 624, 703, 881
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 4 cut(s) 345, 365, 1032, 1144
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 3 cut(s) 87, 226, 242
CfoI GCGC 2 cut(s) 34, 184
Cfr13I GGNCC 6 cut(s) 112, 310, 526, 988, 989, 1204
ClaI ATCGAT 1 cut(s) 553
Csp6I GTAC 3 cut(s) 11, 1044, 1132
CviAII CATG 5 cut(s) 409, 437, 464, 718, 1151
CviQI GTAC 3 cut(s) 11, 1044, 1132
DdeI CTNAG 6 cut(s) 57, 140, 672, 866, 922, 1002
DinI GGCGCC 1 cut(s) 33
DpnI GATC 4 cut(s) 375, 651, 684, 828
DpnII GATC 4 cut(s) 373, 649, 682, 826
Eam1104I CTCTTC 1 cut(s) 1120
EarI CTCTTC 1 cut(s) 1120
Eco130I CCWWGG 1 cut(s) 809
Eco24I GRGCYC 1 cut(s) 992
Eco31I GGTCTC 2 cut(s) 502, 925
Eco47I GGWCC 4 cut(s) 112, 310, 526, 1204
Eco57I CTGAAG 3 cut(s) 105, 711, 1120
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoO109I RGGNCCY 4 cut(s) 310, 988, 989, 1204
EcoRI GAATTC 1 cut(s) 1030
EcoRII CCWGG 2 cut(s) 160, 971
EcoT14I CCWWGG 1 cut(s) 809
EcoT38I GRGCYC 1 cut(s) 992
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 809
FaeI CATG 5 cut(s) 412, 440, 467, 721, 1154
FalI AAGNNNNNCTT 2 cut(s) 1118, 1150
FaqI GGGAC 1 cut(s) 989
FatI CATG 5 cut(s) 408, 436, 463, 717, 1150
FauI CCCGC 1 cut(s) 412
FblI GTMKAC 2 cut(s) 166, 193
Fnu4HI GCNGC 1 cut(s) 747
FokI GGATG 3 cut(s) 631, 690, 888
FriOI GRGCYC 1 cut(s) 992
Fsp4HI GCNGC 1 cut(s) 747
FspBI CTAG 6 cut(s) 47, 86, 585, 662, 810, 1216
GlaI GCGC 2 cut(s) 33, 183
GluI GCNGC 1 cut(s) 747
GsuI CTGGAG 2 cut(s) 624, 654
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 2 cut(s) 793, 990
HapII CCGG 1 cut(s) 206
HhaI GCGC 2 cut(s) 34, 184
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 5 cut(s) 412, 440, 467, 721, 1154
Hin6I GCGC 2 cut(s) 32, 182
HinP1I GCGC 2 cut(s) 32, 182
HindIII AAGCTT 2 cut(s) 764, 941
HinfI GANTC 6 cut(s) 53, 355, 550, 665, 864, 968
HpaII CCGG 1 cut(s) 206
HphI GGTGA 3 cut(s) 577, 589, 907
Hpy166II GTNNAC 6 cut(s) 167, 194, 345, 434, 568, 1016
Hpy188I TCNGA 8 cut(s) 60, 124, 446, 502, 869, 925, 1120, 1180
Hpy188III TCNNGA 3 cut(s) 371, 529, 755
Hpy8I GTNNAC 6 cut(s) 167, 194, 345, 434, 568, 1016
HpyAV CCTTC 4 cut(s) 686, 773, 1079, 1200
HpyCH4III ACNGT 5 cut(s) 15, 116, 191, 258, 562
HpyCH4V TGCA 9 cut(s) 4, 240, 332, 345, 417, 455, 544, 599, 1025
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 1110
HpyF3I CTNAG 6 cut(s) 57, 140, 672, 866, 922, 1002
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 5 cut(s) 412, 440, 467, 721, 1154
HspAI GCGC 2 cut(s) 32, 182
KasI GGCGCC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 373, 649, 682, 826
LguI GCTCTTC 1 cut(s) 1120
LweI GCATC 4 cut(s) 259, 712, 733, 1150
MaeI CTAG 6 cut(s) 47, 86, 585, 662, 810, 1216
MaeIII GTNAC 2 cut(s) 772, 1085
MalI GATC 4 cut(s) 375, 651, 684, 828
MboI GATC 4 cut(s) 373, 649, 682, 826
MboII GAAGA 6 cut(s) 93, 111, 300, 559, 726, 1107
MflI RGATCY 2 cut(s) 649, 682
MhlI GDGCHC 4 cut(s) 42, 347, 473, 992
MluCI AATT 2 cut(s) 841, 1030
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 2 cut(s) 659, 858
MmeI TCCRAC 1 cut(s) 841
MnlI CCTC 9 cut(s) 31, 164, 460, 629, 667, 865, 979, 1002, 1217
MseI TTAA 3 cut(s) 186, 306, 945
MspA1I CMGCKG 2 cut(s) 517, 671
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 162, 973
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 2 cut(s) 162, 973
MwoI GCNNNNNNNGC 2 cut(s) 230, 1110
NarI GGCGCC 1 cut(s) 32
NdeII GATC 4 cut(s) 373, 649, 682, 826
NheI GCTAGC 1 cut(s) 85
NlaIII CATG 5 cut(s) 412, 440, 467, 721, 1154
NlaIV GGNNCC 5 cut(s) 33, 312, 684, 990, 1067
NspI RCATGY 1 cut(s) 1154
PciSI GCTCTTC 1 cut(s) 1120
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 4 cut(s) 53, 355, 550, 968
PkrI GCNGC 1 cut(s) 748
PleI GAGTC 2 cut(s) 659, 858
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 2 cut(s) 659, 858
PpuMI RGGWCCY 2 cut(s) 310, 1204
Psp5II RGGWCCY 2 cut(s) 310, 1204
Psp6I CCWGG 2 cut(s) 160, 971
PspGI CCWGG 2 cut(s) 160, 971
PspN4I GGNNCC 5 cut(s) 33, 312, 684, 990, 1067
PspOMI GGGCCC 1 cut(s) 988
PspPI GGNCC 6 cut(s) 112, 310, 526, 988, 989, 1204
PspPPI RGGWCCY 2 cut(s) 310, 1204
PsuI RGATCY 2 cut(s) 649, 682
RsaI GTAC 3 cut(s) 12, 1045, 1133
RsaNI GTAC 3 cut(s) 11, 1044, 1132
SapI GCTCTTC 1 cut(s) 1120
SaqAI TTAA 3 cut(s) 186, 306, 945
SatI GCNGC 1 cut(s) 747
Sau3AI GATC 4 cut(s) 373, 649, 682, 826
Sau96I GGNCC 6 cut(s) 112, 310, 526, 988, 989, 1204
SchI GAGTC 2 cut(s) 659, 858
ScrFI CCNGG 2 cut(s) 162, 973
SduI GDGCHC 4 cut(s) 42, 347, 473, 992
SfaNI GCATC 4 cut(s) 259, 712, 733, 1150
SfoI GGCGCC 1 cut(s) 33
SinI GGWCC 4 cut(s) 112, 310, 526, 1204
SmlI CTYRAG 3 cut(s) 41, 944, 1093
SmoI CTYRAG 3 cut(s) 41, 944, 1093
Sse9I AATT 2 cut(s) 841, 1030
SsiI CCGC 5 cut(s) 89, 405, 515, 669, 746
SspDI GGCGCC 1 cut(s) 31
SspMI CTAG 6 cut(s) 47, 86, 585, 662, 810, 1216
StyD4I CCNGG 2 cut(s) 160, 971
StyI CCWWGG 1 cut(s) 809
TaaI ACNGT 5 cut(s) 15, 116, 191, 258, 562
TaqI TCGA 3 cut(s) 286, 553, 756
TasI AATT 2 cut(s) 841, 1030
TatI WGTACW 1 cut(s) 1043
TauI GCSGC 1 cut(s) 749
TfiI GAWTC 4 cut(s) 53, 355, 550, 968
Tru1I TTAA 3 cut(s) 186, 306, 945
Tru9I TTAA 3 cut(s) 186, 306, 945
TscAI CASTG 4 cut(s) 352, 365, 1032, 1144
TspDTI ATGAA 3 cut(s) 552, 953, 1061
TspGWI ACGGA 1 cut(s) 791
TspRI CASTG 4 cut(s) 352, 365, 1032, 1144
Vha464I CTTAAG 1 cut(s) 944
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 4 cut(s) 112, 310, 526, 1204
XagI CCTNNNNNAGG 1 cut(s) 690
XapI RAATTY 1 cut(s) 1030
XceI RCATGY 1 cut(s) 1154
XcmI CCANNNNNNNNNTGG 2 cut(s) 344, 715
XmaJI CCTAGG 1 cut(s) 809
XmiI GTMKAC 2 cut(s) 166, 193
XspI CTAG 6 cut(s) 47, 86, 585, 662, 810, 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.