MD10G1261300.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011247
Physical Location & Seq
Forward (+)
35557384 .. 35560891
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1261300.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGCAACGGGTTAGATTGTCCTCCCAGCAGGCGCCGGTACACAAGCTAGGGGATTCTCAAATTGCATTATCCCCGAAGTTTAGGTTAGCGGTTATCCAGTCTTCGTTGTTGAATCCTTCCCCGGCATTAGAGTTATCTCTTGAGGAAAACTCCCTCATTCCTGGCCTTCCTGATGATGTTGCACTTAACTGCCTCCTTCGGGTTCCAGTCGAAAGCCACACAGCAGGCAAAGCTGTCTGCAAGCGTTGGCATCTATTGTTTGGTAGTAAAGAGCGGTTTTTTACTCGAAGAAAACAATTAGGCTTCAAAGACCCTTGGCTTTTTGTCTTTGCCTTTCACAAGTGCACTGGAAGGATTCAGTGGCAGGTTCTTGATCTCACTAATTTCTCTTGGCACACTATCCCTCCCATGCCCTGCAGAGACAAGGTTTTCCCCCATGGTTTCAGATGTGTTTCGATGCCCCATGAAGGAACTCTCTTTATTTGCGGTGGTATGGTCTCTGATGTGGATTGTCCGCTTGACTTGGTCCTGAAATACGAAGTTCAGAAGAATCGGTGGACTGTAATAAATCAGATGATAAGTGCTAGGTCTTTTTTTGCAAGTGGAGTTATTGATGGGAAGATTTATGTGGCTGGAGGAAACAGTTCAGATCTTTTTGAGCTTGACTCAGCCGAGGTTTTGGATCCTGTTAAAGGGAGTTGGCATCCCATAGCTAGCATGGGAGCAAATATGGCCTCTTATGATGCAGCAGTTCTCAACGGAAAGCTTCTTGTTACAGAAGGCTGGTTGTGGCCTTTCTTTGTCTCTCCTAGGGGTCAGGTTTATGACCCGAGAGCCAATAATTGGGAGAGCATGGCTGTTGGACTGAGAGAAGGCTGGACAGGTTCGAGTGTGGTGATTTATGGCCATTTGTTTGTGGTCTCGGAGCTTGAAAGGATGAAGCTTAAGGTTTATGATGCGGATACAGATTCTTGGGAACCAATAGAAGGACCCCCTTTGCCAGAGCAGATCTGTAAACCCTTTGCTGTTAATGCCTGTGATTGCATGATATATGTTGTGGGTAGGAACCTTCATGTTGCTGTCGGTCAAATCTCAAGGGTTAGCCATAAGGGAACTTGCGAGAAGAAGTGGAGCTTTGGTGTTCGATGGAATGTGGTTGATGCGCCAGATTGTTTCTCTGACTTAACGCCCTCAAGTTCACAGGTCCTGTTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

406

Amino Acids

45.25

Weight (kDa)

7.13

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 121 - 217 8.3e-06 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 122 - 286 4.3e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 162 - 284 1.4e-07 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 172 - 331 1.6e-18 FKB95, Kelch-repeats domain
Kelch_1 PF01344 195 - 241 5.8e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 355
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 843
AccBSI CCGCTC 1 cut(s) 274
AciI CCGC 5 cut(s) 89, 274, 486, 515, 959
AclWI GGATC 2 cut(s) 677, 690
AcoI YGGCCR 1 cut(s) 904
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 5 cut(s) 199, 467, 692, 843, 986
AflII CTTAAG 1 cut(s) 944
AgsI TTSAA 3 cut(s) 112, 307, 932
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 8 cut(s) 46, 233, 661, 713, 766, 928, 943, 1134
AluI AGCT 8 cut(s) 46, 233, 661, 713, 766, 928, 943, 1134
Alw21I GWGCWC 1 cut(s) 347
Alw26I GTCTC 4 cut(s) 414, 502, 808, 925
Alw44I GTGCAC 1 cut(s) 343
AlwI GGATC 2 cut(s) 677, 690
AlwNI CAGNNNCTG 1 cut(s) 1207
Ama87I CYCGRG 1 cut(s) 829
AoxI GGCC 4 cut(s) 163, 732, 791, 904
ApaLI GTGCAC 1 cut(s) 343
ApeKI GCWGC 1 cut(s) 746
Asp700I GAANNNNTTC 1 cut(s) 643
AspA2I CCTAGG 1 cut(s) 809
AspLEI GCGC 2 cut(s) 34, 1165
AspS9I GGNCC 3 cut(s) 526, 989, 1204
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 907
AsuNHI GCTAGC 1 cut(s) 713
AvaI CYCGRG 1 cut(s) 829
AvaII GGWCC 3 cut(s) 526, 989, 1204
AvrII CCTAGG 1 cut(s) 809
BaeGI GKGCMC 1 cut(s) 347
BalI TGGCCA 1 cut(s) 906
BamHI GGATCC 1 cut(s) 682
BanI GGYRCC 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 347
BbvI GCAGC 1 cut(s) 758
BccI CCATC 2 cut(s) 608, 1140
BciT130I CCWGG 1 cut(s) 162
BciVI GTATCC 1 cut(s) 955
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 4 cut(s) 414, 502, 808, 925
BfaI CTAG 4 cut(s) 47, 585, 714, 810
BfmI CTRYAG 1 cut(s) 415
BfoI RGCGCY 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 944
BfuAI ACCTGC 1 cut(s) 355
BfuI GTATCC 1 cut(s) 955
BglII AGATCT 2 cut(s) 649, 1008
BisI GCNGC 1 cut(s) 747
BlnI CCTAGG 1 cut(s) 809
BlsI GCNGC 1 cut(s) 748
Bme1390I CCNGG 2 cut(s) 122, 162
Bme18I GGWCC 3 cut(s) 526, 989, 1204
BmeT110I CYCGRG 1 cut(s) 829
BmgT120I GGNCC 3 cut(s) 526, 989, 1204
BmiI GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 1067
BmrFI CCNGG 2 cut(s) 122, 162
BmsI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
BmtI GCTAGC 1 cut(s) 717
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 4 cut(s) 134, 166, 650, 682
BpmI CTGGAG 1 cut(s) 654
BpuEI CTTGAG 3 cut(s) 161, 1078, 1177
BpuMI CCSGG 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 2 cut(s) 502, 925
BsaJI CCNNGG 5 cut(s) 120, 314, 436, 672, 809
BsaXI ACNNNNNCTCC 4 cut(s) 388, 418, 1123, 1153
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 467, 692, 843, 986
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 97, 206, 352
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 5 cut(s) 120, 314, 436, 672, 809
BseGI GGATG 2 cut(s) 703, 942
BseLI CCNNNNNNNGG 5 cut(s) 199, 467, 692, 843, 986
BseMII CTCAG 2 cut(s) 681, 857
BseNI ACTGG 3 cut(s) 97, 206, 352
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 1 cut(s) 758
BseYI CCCAGC 1 cut(s) 24
BshFI GGCC 4 cut(s) 165, 734, 793, 906
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 347
BsiHKCI CYCGRG 1 cut(s) 829
BsiSI CCGG 2 cut(s) 35, 122
BslI CCNNNNNNNGG 5 cut(s) 199, 467, 692, 843, 986
BsmAI GTCTC 4 cut(s) 414, 502, 808, 925
BsnI GGCC 4 cut(s) 165, 734, 793, 906
Bso31I GGTCTC 2 cut(s) 502, 925
BsoBI CYCGRG 1 cut(s) 829
Bsp1286I GDGCHC 1 cut(s) 347
Bsp143I GATC 4 cut(s) 373, 649, 682, 1008
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 5 cut(s) 89, 274, 486, 515, 959
BspANI GGCC 4 cut(s) 165, 734, 793, 906
BspCNI CTCAG 2 cut(s) 680, 858
BspLI GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 1067
BspMAI CTGCAG 1 cut(s) 419
BspMI ACCTGC 1 cut(s) 355
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 2 cut(s) 677, 690
BspT107I GGYRCC 1 cut(s) 31
BspTI CTTAAG 1 cut(s) 944
BspTNI GGTCTC 2 cut(s) 502, 925
BsrBI CCGCTC 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 3 cut(s) 97, 206, 352
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 5 cut(s) 120, 314, 436, 672, 809
BssMI GATC 4 cut(s) 373, 649, 682, 1008
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 3 cut(s) 314, 436, 809
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 562, 644
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 944
BstC8I GCNNGC 4 cut(s) 30, 226, 242, 715
BstDEI CTNAG 3 cut(s) 667, 866, 1215
BstDSI CCRYGG 1 cut(s) 436
BstENI CCTNNNNNAGG 1 cut(s) 690
BstF5I GGATG 2 cut(s) 703, 942
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 2 cut(s) 34, 1165
BstKTI GATC 4 cut(s) 376, 652, 685, 1011
BstMAI GTCTC 4 cut(s) 414, 502, 808, 925
BstMBI GATC 4 cut(s) 373, 649, 682, 1008
BstMWI GCNNNNNNNGC 3 cut(s) 230, 731, 1031
BstNI CCWGG 1 cut(s) 162
BstSCI CCNGG 2 cut(s) 120, 160
BstSFI CTRYAG 1 cut(s) 415
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 758
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 3 cut(s) 649, 682, 1008
BstYI RGATCY 3 cut(s) 649, 682, 1008
BsuI GTATCC 1 cut(s) 955
BsuRI GGCC 4 cut(s) 165, 734, 793, 906
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 2 cut(s) 703, 942
BtsIMutI CAGTG 2 cut(s) 345, 365
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 4 cut(s) 30, 226, 242, 715
CaiI CAGNNNCTG 1 cut(s) 1207
CfoI GCGC 2 cut(s) 34, 1165
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 526, 989, 1204
Csp6I GTAC 1 cut(s) 38
CviAII CATG 7 cut(s) 409, 437, 464, 718, 853, 1045, 1073
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 3 cut(s) 667, 866, 1215
DinI GGCGCC 1 cut(s) 33
DpnI GATC 4 cut(s) 375, 651, 684, 1010
DpnII GATC 4 cut(s) 373, 649, 682, 1008
EaeI YGGCCR 1 cut(s) 904
Eco130I CCWWGG 3 cut(s) 314, 436, 809
Eco31I GGTCTC 2 cut(s) 502, 925
Eco47I GGWCC 3 cut(s) 526, 989, 1204
Eco88I CYCGRG 1 cut(s) 829
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoO109I RGGNCCY 2 cut(s) 989, 1204
EcoRII CCWGG 1 cut(s) 160
EcoT14I CCWWGG 3 cut(s) 314, 436, 809
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 3 cut(s) 314, 436, 809
FaeI CATG 7 cut(s) 412, 440, 467, 721, 856, 1048, 1076
FalI AAGNNNNNCTT 2 cut(s) 1118, 1150
FatI CATG 7 cut(s) 408, 436, 463, 717, 852, 1044, 1072
Fnu4HI GCNGC 1 cut(s) 747
FokI GGATG 2 cut(s) 690, 949
Fsp4HI GCNGC 1 cut(s) 747
FspBI CTAG 4 cut(s) 47, 585, 714, 810
GlaI GCGC 2 cut(s) 33, 1164
GluI GCNGC 1 cut(s) 747
GsaI CCCAGC 1 cut(s) 28
GsuI CTGGAG 1 cut(s) 654
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 4 cut(s) 165, 734, 793, 906
HapII CCGG 2 cut(s) 35, 122
HhaI GCGC 2 cut(s) 34, 1165
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 7 cut(s) 412, 440, 467, 721, 856, 1048, 1076
Hin6I GCGC 2 cut(s) 32, 1163
HinP1I GCGC 2 cut(s) 32, 1163
HindIII AAGCTT 2 cut(s) 764, 941
HinfI GANTC 6 cut(s) 53, 112, 355, 550, 665, 968
HpaII CCGG 2 cut(s) 35, 122
HphI GGTGA 1 cut(s) 907
Hpy166II GTNNAC 5 cut(s) 40, 345, 558, 1016, 1199
Hpy188I TCNGA 7 cut(s) 446, 502, 546, 573, 649, 925, 1180
Hpy188III TCNNGA 4 cut(s) 140, 170, 371, 529
Hpy8I GTNNAC 5 cut(s) 40, 345, 558, 1016, 1199
HpyAV CCTTC 9 cut(s) 126, 176, 206, 345, 461, 773, 866, 980, 1079
HpyCH4III ACNGT 2 cut(s) 562, 644
HpyCH4V TGCA 9 cut(s) 4, 65, 182, 240, 345, 417, 599, 746, 1044
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 731, 1031
HpyF3I CTNAG 3 cut(s) 667, 866, 1215
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 7 cut(s) 412, 440, 467, 721, 856, 1048, 1076
HspAI GCGC 2 cut(s) 32, 1163
KasI GGCGCC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 373, 649, 682, 1008
LmnI GCTCC 3 cut(s) 722, 925, 1131
Lsp1109I GCAGC 1 cut(s) 758
LweI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
MaeI CTAG 4 cut(s) 47, 585, 714, 810
MaeIII GTNAC 1 cut(s) 772
MalI GATC 4 cut(s) 375, 651, 684, 1010
MbiI CCGCTC 1 cut(s) 274
MboI GATC 4 cut(s) 373, 649, 682, 1008
MboII GAAGA 5 cut(s) 93, 300, 559, 631, 1135
MflI RGATCY 3 cut(s) 649, 682, 1008
MhlI GDGCHC 1 cut(s) 347
MlsI TGGCCA 1 cut(s) 906
MluCI AATT 4 cut(s) 60, 296, 382, 841
MluNI TGGCCA 1 cut(s) 906
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 1 cut(s) 841
MnlI CCTC 9 cut(s) 31, 136, 164, 203, 414, 629, 667, 745, 1201
Mox20I TGGCCA 1 cut(s) 906
MroXI GAANNNNTTC 1 cut(s) 643
MscI TGGCCA 1 cut(s) 906
MseI TTAA 5 cut(s) 186, 690, 945, 1029, 1184
Msp20I TGGCCA 1 cut(s) 906
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 2 cut(s) 35, 122
MspR9I CCNGG 2 cut(s) 122, 162
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 230, 731, 1031
NarI GGCGCC 1 cut(s) 32
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 436
NdeII GATC 4 cut(s) 373, 649, 682, 1008
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 7 cut(s) 412, 440, 467, 721, 856, 1048, 1076
NlaIV GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 1067
NmeAIII GCCGAG 1 cut(s) 697
PdmI GAANNNNTTC 1 cut(s) 643
PfeI GAWTC 5 cut(s) 53, 112, 355, 550, 968
PflFI GACNNNGTC 1 cut(s) 524
PflMI CCANNNNNTGG 1 cut(s) 843
PkrI GCNGC 1 cut(s) 748
PleI GAGTC 1 cut(s) 659
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 659
PpuMI RGGWCCY 2 cut(s) 989, 1204
Psp5II RGGWCCY 2 cut(s) 989, 1204
Psp6I CCWGG 1 cut(s) 160
PspFI CCCAGC 1 cut(s) 24
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 1067
PspPI GGNCC 3 cut(s) 526, 989, 1204
PspPPI RGGWCCY 2 cut(s) 989, 1204
PstI CTGCAG 1 cut(s) 419
PstNI CAGNNNCTG 1 cut(s) 1207
PsuI RGATCY 3 cut(s) 649, 682, 1008
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
SaqAI TTAA 5 cut(s) 186, 690, 945, 1029, 1184
SatI GCNGC 1 cut(s) 747
Sau3AI GATC 4 cut(s) 373, 649, 682, 1008
Sau96I GGNCC 3 cut(s) 526, 989, 1204
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 2 cut(s) 122, 162
SduI GDGCHC 1 cut(s) 347
SfaNI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
SfcI CTRYAG 1 cut(s) 415
SfoI GGCGCC 1 cut(s) 33
SinI GGWCC 3 cut(s) 526, 989, 1204
SmlI CTYRAG 4 cut(s) 140, 944, 1093, 1192
SmoI CTYRAG 4 cut(s) 140, 944, 1093, 1192
Sse9I AATT 4 cut(s) 60, 296, 382, 841
SsiI CCGC 5 cut(s) 89, 274, 486, 515, 959
SspDI GGCGCC 1 cut(s) 31
SspMI CTAG 4 cut(s) 47, 585, 714, 810
StyD4I CCNGG 2 cut(s) 120, 160
StyI CCWWGG 3 cut(s) 314, 436, 809
TaaI ACNGT 2 cut(s) 562, 644
TaqI TCGA 5 cut(s) 210, 286, 455, 887, 1144
TaqII GACCGA 1 cut(s) 1073
TasI AATT 4 cut(s) 60, 296, 382, 841
TfiI GAWTC 5 cut(s) 53, 112, 355, 550, 968
Tru1I TTAA 5 cut(s) 186, 690, 945, 1029, 1184
Tru9I TTAA 5 cut(s) 186, 690, 945, 1029, 1184
TscAI CASTG 2 cut(s) 352, 365
TseI GCWGC 1 cut(s) 746
TspDTI ATGAA 3 cut(s) 480, 953, 1061
TspGWI ACGGA 1 cut(s) 774
TspRI CASTG 2 cut(s) 352, 365
Tth111I GACNNNGTC 1 cut(s) 524
Van91I CCANNNNNTGG 1 cut(s) 843
Vha464I CTTAAG 1 cut(s) 944
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 3 cut(s) 526, 989, 1204
XagI CCTNNNNNAGG 1 cut(s) 690
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XmaJI CCTAGG 1 cut(s) 809
XmnI GAANNNNTTC 1 cut(s) 643
XspI CTAG 4 cut(s) 47, 585, 714, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.