Rroxscaffold_1G00062280

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011247
Physical Location & Seq
Forward (+)
84338121 .. 84339337
1217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062280.1

Sequence Viewer

Length: 1164 bp
ATGCAACGAGTTCGATTGTCCTCTCAACAGGCGCCTGTGCTCAAGCTAGGGGATTCTCAGATCGCATTATCTCCAAAATTTAGGCTAGCGGGAGAACCCCTTATTCCTGGTCTACCTGATGATGTGGCGCTTAACTGTCTCCTTCGGCTTCCTGTGGAAAGCCATACAGCAAGCAAAGCTGTATGCAAGCGTTGGCATCTACTGCTTGGTAGTAAAGAGCGGTTTTTCACTCGTAGAAAAGAAGTAGGGTTTAAAGACCCGTGGCTTTTTGTCTTTGCATTCCACAAGTGCACTGGAAAGATTCAGTGGCAGGTTCTTGATCTCACTCATTTCTCGTGGCACACAATCCCTGCCATGCCTTGCAAAGACAAAGTTTGTCCCCATGGGTTCAGATGTGTTGCAATTCCCCATGAGGGCACTCTCTTTGTTTGTGGTGGTATGGTCTCTGATGTGGATTGTCCGCTGGATTTGGTCCTGAAATATGAAATGCAGAAGAATCGATGGACTGTGGTGAACCAGATGGTGAGTGCCAGGTCTTTTTTTGCAAGCGGAGTGATTGATGGGATGATTTATGTGGCTGGAGGAAACAGCGCAGATCTTTTTGAGCTCGACTCCGCTGAGGTTTTGGACCCTGCTGAAGGGAGTTGGCGTTCCATTGCGAGCATGGGAACAAATATGTCCTCTTATGATGCAGCAGTACTCGATGGAAAGCTTCTTGTTACGGAAGGCTGGTTGTGGCCTTTCTATGTTTCTCCTAGGGGTCAGGTTTATGATCCAAGAACTGATAATTGGGAGAGTATGGCTGTTGGACTCAGAGAGGGATGGACGGGTTCAAGTGTGGTGATTTACGGTCATTTGTTTGTGGTCTCAGAGCTTGAAAGAATGAAGCTTAAGGTTTATGACGTGGATACCGATTCCTGGGAACCAATAGATGGCCTTCCTTTACCTGAGCAGATATGTAAACCCTTTGCAGTGAATTCTTGTGACTGCACAATATATGTCGTTGGTAGGAACCTTCATGTTGCTGTTGGTAACATCTCAAGGGTTAGCCGTAAAGGCTCTTCTGAGAACAAGTGGCACTTCAGTGTTCGATGGCATGTGGTTGATGCGCCAGATAATTTGTCTGACTTGACACCTTCAAGCTCACAGGTCCTCTTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

387

Amino Acids

43.25

Weight (kDa)

6.42

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 104 - 269 8.5e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 104 - 198 1e-05 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 141 - 319 1.4e-18 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 143 - 266 1.2e-08 Calicin, beta-propeller domain
Kelch_1 PF01344 177 - 223 4.4e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 932
AccBSI CCGCTC 1 cut(s) 220
AccI GTMKAC 1 cut(s) 112
AciI CCGC 5 cut(s) 89, 220, 461, 549, 615
AclWI GGATC 1 cut(s) 767
AcsI RAATTY 2 cut(s) 77, 976
AcuI CTGAAG 2 cut(s) 657, 1066
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 1 cut(s) 699
AfiI CCNNNNNNNGG 4 cut(s) 413, 638, 918, 932
AflII CTTAAG 1 cut(s) 890
AgsI TTSAA 3 cut(s) 834, 878, 1140
AjiI CACGTC 1 cut(s) 904
AjnI CCWGG 3 cut(s) 106, 530, 917
AjuI GAANNNNNNNTTGG 2 cut(s) 772, 804
AleI CACNNNNGTG 1 cut(s) 1083
AloI GAACNNNNNNTCC 2 cut(s) 634, 666
AluBI AGCT 7 cut(s) 46, 179, 607, 712, 874, 889, 1143
AluI AGCT 7 cut(s) 46, 179, 607, 712, 874, 889, 1143
Alw21I GWGCWC 3 cut(s) 42, 293, 609
Alw26I GTCTC 3 cut(s) 143, 448, 871
Alw44I GTGCAC 1 cut(s) 289
AlwI GGATC 1 cut(s) 767
AoxI GGCC 2 cut(s) 737, 934
ApaLI GTGCAC 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 692
ApoI RAATTY 2 cut(s) 77, 976
AspA2I CCTAGG 1 cut(s) 755
AspLEI GCGC 4 cut(s) 34, 130, 593, 1111
AspS9I GGNCC 3 cut(s) 472, 628, 1150
AsuHPI GGTGA 3 cut(s) 523, 535, 853
AsuNHI GCTAGC 1 cut(s) 85
AvaII GGWCC 3 cut(s) 472, 628, 1150
AvrII CCTAGG 1 cut(s) 755
BaeGI GKGCMC 2 cut(s) 293, 419
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 609
BarI GAAGNNNNNNTAC 2 cut(s) 1045, 1077
BauI CACGAG 1 cut(s) 334
Bbv12I GWGCWC 3 cut(s) 42, 293, 609
BbvCI CCTCAGC 1 cut(s) 618
BbvI GCAGC 1 cut(s) 704
BccI CCATC 7 cut(s) 495, 514, 554, 698, 816, 926, 1086
BceAI ACGGC 1 cut(s) 1035
BcgI CGANNNNNNTGC 3 cut(s) 27, 479, 513
BciT130I CCWGG 3 cut(s) 108, 532, 919
BciVI GTATCC 1 cut(s) 901
BcoDI GTCTC 3 cut(s) 143, 448, 871
BfaI CTAG 4 cut(s) 47, 86, 756, 1162
BfoI RGCGCY 2 cut(s) 35, 131
BfrI CTTAAG 1 cut(s) 890
BfuAI ACCTGC 1 cut(s) 301
BfuI GTATCC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 1056
BglII AGATCT 1 cut(s) 595
BisI GCNGC 1 cut(s) 693
BlnI CCTAGG 1 cut(s) 755
BlsI GCNGC 1 cut(s) 694
BmcAI AGTACT 1 cut(s) 699
Bme1390I CCNGG 3 cut(s) 108, 532, 919
Bme18I GGWCC 3 cut(s) 472, 628, 1150
BmgBI CACGTC 1 cut(s) 904
BmgT120I GGNCC 3 cut(s) 472, 628, 1150
BmiI GGNNCC 4 cut(s) 33, 630, 924, 1013
BmrFI CCNGG 3 cut(s) 108, 532, 919
BmsI GCATC 3 cut(s) 205, 679, 1096
BmtI GCTAGC 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BpmI CTGGAG 1 cut(s) 600
Bpu10I CCTNAGC 2 cut(s) 618, 948
BpuEI CTTGAG 2 cut(s) 26, 1024
Bsa29I ATCGAT 1 cut(s) 499
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 2 cut(s) 448, 871
BsaJI CCNNGG 4 cut(s) 260, 382, 755, 918
BsaXI ACNNNNNCTCC 2 cut(s) 634, 664
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 638, 918, 932
Bse1I ACTGG 1 cut(s) 298
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 3 cut(s) 108, 532, 919
BseCI ATCGAT 1 cut(s) 499
BseDI CCNNGG 4 cut(s) 260, 382, 755, 918
BseGI GGATG 2 cut(s) 570, 827
BseLI CCNNNNNNNGG 4 cut(s) 413, 638, 918, 932
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 6 cut(s) 71, 609, 826, 882, 939, 1056
BseNI ACTGG 1 cut(s) 298
BseSI GKGCMC 2 cut(s) 293, 419
BseXI GCAGC 1 cut(s) 704
BsgI GTGCAG 1 cut(s) 973
BshFI GGCC 2 cut(s) 739, 936
BshNI GGYRCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 499
BsiHKAI GWGCWC 3 cut(s) 42, 293, 609
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 4 cut(s) 413, 638, 918, 932
BsmAI GTCTC 3 cut(s) 143, 448, 871
BsmFI GGGAC 1 cut(s) 363
BsmI GAATGC 1 cut(s) 278
BsnI GGCC 2 cut(s) 739, 936
Bso31I GGTCTC 2 cut(s) 448, 871
Bsp1286I GDGCHC 4 cut(s) 42, 293, 419, 609
Bsp143I GATC 4 cut(s) 60, 319, 595, 772
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 5 cut(s) 89, 220, 461, 549, 615
BspANI GGCC 2 cut(s) 739, 936
BspCNI CTCAG 6 cut(s) 70, 610, 825, 881, 940, 1057
BspDI ATCGAT 1 cut(s) 499
BspLI GGNNCC 4 cut(s) 33, 630, 924, 1013
BspMI ACCTGC 1 cut(s) 301
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 1 cut(s) 767
BspQI GCTCTTC 1 cut(s) 1066
BspT107I GGYRCC 1 cut(s) 31
BspTI CTTAAG 1 cut(s) 890
BspTNI GGTCTC 2 cut(s) 448, 871
BsrBI CCGCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 654
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 4 cut(s) 260, 382, 755, 918
BssMI GATC 4 cut(s) 60, 319, 595, 772
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 1 cut(s) 334
BssT1I CCWWGG 2 cut(s) 382, 755
Bst2BI CACGAG 1 cut(s) 334
Bst2UI CCWGG 3 cut(s) 108, 532, 919
Bst4CI ACNGT 3 cut(s) 137, 508, 851
Bst6I CTCTTC 1 cut(s) 1066
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 890
BstAPI GCANNNNNTGC 1 cut(s) 202
BstC8I GCNNGC 5 cut(s) 87, 172, 188, 547, 661
BstDEI CTNAG 6 cut(s) 57, 618, 812, 868, 948, 1065
BstDSI CCRYGG 2 cut(s) 260, 382
BstENI CCTNNNNNAGG 1 cut(s) 636
BstF5I GGATG 2 cut(s) 570, 827
BstH2I RGCGCY 2 cut(s) 35, 131
BstHHI GCGC 4 cut(s) 34, 130, 593, 1111
BstKTI GATC 4 cut(s) 63, 322, 598, 775
BstMAI GTCTC 3 cut(s) 143, 448, 871
BstMBI GATC 4 cut(s) 60, 319, 595, 772
BstMWI GCNNNNNNNGC 3 cut(s) 176, 202, 1056
BstNI CCWGG 3 cut(s) 108, 532, 919
BstNSI RCATGY 1 cut(s) 1100
BstSCI CCNGG 3 cut(s) 106, 530, 917
BstSLI GKGCMC 2 cut(s) 293, 419
BstV1I GCAGC 1 cut(s) 704
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
Bsu15I ATCGAT 1 cut(s) 499
BsuI GTATCC 1 cut(s) 901
BsuRI GGCC 2 cut(s) 739, 936
BsuTUI ATCGAT 1 cut(s) 499
BtgI CCRYGG 2 cut(s) 260, 382
BtrI CACGTC 1 cut(s) 904
BtsCI GGATG 2 cut(s) 570, 827
BtsI GCAGTG 1 cut(s) 978
BtsIMutI CAGTG 4 cut(s) 291, 311, 978, 1090
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 5 cut(s) 87, 172, 188, 547, 661
CfoI GCGC 4 cut(s) 34, 130, 593, 1111
Cfr13I GGNCC 3 cut(s) 472, 628, 1150
ClaI ATCGAT 1 cut(s) 499
Csp6I GTAC 1 cut(s) 698
CviAII CATG 6 cut(s) 355, 383, 410, 664, 1019, 1097
CviQI GTAC 1 cut(s) 698
DdeI CTNAG 6 cut(s) 57, 618, 812, 868, 948, 1065
DinI GGCGCC 1 cut(s) 33
DpnI GATC 4 cut(s) 62, 321, 597, 774
DpnII GATC 4 cut(s) 60, 319, 595, 772
DraI TTTAAA 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 1066
EarI CTCTTC 1 cut(s) 1066
Ecl136II GAGCTC 1 cut(s) 607
Eco130I CCWWGG 2 cut(s) 382, 755
Eco24I GRGCYC 1 cut(s) 609
Eco31I GGTCTC 2 cut(s) 448, 871
Eco47I GGWCC 3 cut(s) 472, 628, 1150
Eco53kI GAGCTC 1 cut(s) 607
Eco57I CTGAAG 2 cut(s) 657, 1066
EcoICRI GAGCTC 1 cut(s) 607
EcoNI CCTNNNNNAGG 1 cut(s) 636
EcoO109I RGGNCCY 1 cut(s) 1150
EcoRI GAATTC 1 cut(s) 976
EcoRII CCWGG 3 cut(s) 106, 530, 917
EcoT14I CCWWGG 2 cut(s) 382, 755
EcoT38I GRGCYC 1 cut(s) 609
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 382, 755
FaeI CATG 6 cut(s) 358, 386, 413, 667, 1022, 1100
FalI AAGNNNNNCTT 2 cut(s) 1064, 1096
FaqI GGGAC 1 cut(s) 363
FatI CATG 6 cut(s) 354, 382, 409, 663, 1018, 1096
FauI CCCGC 1 cut(s) 82
FblI GTMKAC 1 cut(s) 112
Fnu4HI GCNGC 1 cut(s) 693
FokI GGATG 2 cut(s) 577, 834
FriOI GRGCYC 1 cut(s) 609
Fsp4HI GCNGC 1 cut(s) 693
FspBI CTAG 4 cut(s) 47, 86, 756, 1162
GlaI GCGC 4 cut(s) 33, 129, 592, 1110
GluI GCNGC 1 cut(s) 693
GsuI CTGGAG 1 cut(s) 600
HaeII RGCGCY 2 cut(s) 35, 131
HaeIII GGCC 2 cut(s) 739, 936
HhaI GCGC 4 cut(s) 34, 130, 593, 1111
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 6 cut(s) 358, 386, 413, 667, 1022, 1100
Hin6I GCGC 4 cut(s) 32, 128, 591, 1109
HinP1I GCGC 4 cut(s) 32, 128, 591, 1109
HindIII AAGCTT 2 cut(s) 710, 887
HinfI GANTC 6 cut(s) 53, 301, 496, 611, 810, 914
HphI GGTGA 3 cut(s) 523, 535, 853
Hpy166II GTNNAC 4 cut(s) 113, 291, 514, 962
Hpy188I TCNGA 7 cut(s) 60, 392, 448, 815, 871, 1066, 1126
Hpy188III TCNNGA 2 cut(s) 317, 475
Hpy8I GTNNAC 4 cut(s) 113, 291, 514, 962
HpyAV CCTTC 6 cut(s) 152, 632, 719, 947, 1025, 1146
HpyCH4III ACNGT 3 cut(s) 137, 508, 851
HpyCH4IV ACGT 1 cut(s) 903
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 202, 1056
HpyF3I CTNAG 6 cut(s) 57, 618, 812, 868, 948, 1065
HpySE526I ACGT 1 cut(s) 903
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 6 cut(s) 358, 386, 413, 667, 1022, 1100
HspAI GCGC 4 cut(s) 32, 128, 591, 1109
KasI GGCGCC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 60, 319, 595, 772
LguI GCTCTTC 1 cut(s) 1066
Lsp1109I GCAGC 1 cut(s) 704
LweI GCATC 3 cut(s) 205, 679, 1096
MaeI CTAG 4 cut(s) 47, 86, 756, 1162
MaeII ACGT 1 cut(s) 903
MaeIII GTNAC 3 cut(s) 718, 983, 1031
MalI GATC 4 cut(s) 62, 321, 597, 774
MbiI CCGCTC 1 cut(s) 220
MboI GATC 4 cut(s) 60, 319, 595, 772
MboII GAAGA 2 cut(s) 505, 1053
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 4 cut(s) 42, 293, 419, 609
MluCI AATT 5 cut(s) 77, 402, 787, 976, 1117
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 2 cut(s) 605, 804
MmeI TCCRAC 1 cut(s) 787
MnlI CCTC 7 cut(s) 31, 406, 575, 613, 691, 811, 1163
MseI TTAA 3 cut(s) 132, 252, 891
MslI CAYNNNNRTG 1 cut(s) 1083
MspA1I CMGCKG 2 cut(s) 463, 617
MspCI CTTAAG 1 cut(s) 890
MspR9I CCNGG 3 cut(s) 108, 532, 919
Mva1269I GAATGC 1 cut(s) 278
MvaI CCWGG 3 cut(s) 108, 532, 919
MwoI GCNNNNNNNGC 3 cut(s) 176, 202, 1056
NarI GGCGCC 1 cut(s) 32
NcoI CCATGG 1 cut(s) 382
NdeII GATC 4 cut(s) 60, 319, 595, 772
NheI GCTAGC 1 cut(s) 85
NlaIII CATG 6 cut(s) 358, 386, 413, 667, 1022, 1100
NlaIV GGNNCC 4 cut(s) 33, 630, 924, 1013
NmuCI GTSAC 1 cut(s) 983
NspI RCATGY 1 cut(s) 1100
OliI CACNNNNGTG 1 cut(s) 1083
PciSI GCTCTTC 1 cut(s) 1066
PcsI WCGNNNNNNNCGW 1 cut(s) 909
PctI GAATGC 1 cut(s) 278
PfeI GAWTC 4 cut(s) 53, 301, 496, 914
PflMI CCANNNNNTGG 1 cut(s) 932
PkrI GCNGC 1 cut(s) 694
PleI GAGTC 2 cut(s) 605, 804
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 2 cut(s) 605, 804
PpuMI RGGWCCY 1 cut(s) 1150
Psp124BI GAGCTC 1 cut(s) 609
Psp5II RGGWCCY 1 cut(s) 1150
Psp6I CCWGG 3 cut(s) 106, 530, 917
PspGI CCWGG 3 cut(s) 106, 530, 917
PspN4I GGNNCC 4 cut(s) 33, 630, 924, 1013
PspPI GGNCC 3 cut(s) 472, 628, 1150
PspPPI RGGWCCY 1 cut(s) 1150
PsuI RGATCY 1 cut(s) 595
RsaI GTAC 1 cut(s) 699
RsaNI GTAC 1 cut(s) 698
RseI CAYNNNNRTG 1 cut(s) 1083
SacI GAGCTC 1 cut(s) 609
SapI GCTCTTC 1 cut(s) 1066
SaqAI TTAA 3 cut(s) 132, 252, 891
SatI GCNGC 1 cut(s) 693
Sau3AI GATC 4 cut(s) 60, 319, 595, 772
Sau96I GGNCC 3 cut(s) 472, 628, 1150
ScaI AGTACT 1 cut(s) 699
SchI GAGTC 2 cut(s) 605, 804
ScrFI CCNGG 3 cut(s) 108, 532, 919
SduI GDGCHC 4 cut(s) 42, 293, 419, 609
SfaNI GCATC 3 cut(s) 205, 679, 1096
SfoI GGCGCC 1 cut(s) 33
SinI GGWCC 3 cut(s) 472, 628, 1150
SmiMI CAYNNNNRTG 1 cut(s) 1083
SmlI CTYRAG 3 cut(s) 41, 890, 1039
SmoI CTYRAG 3 cut(s) 41, 890, 1039
Sse9I AATT 5 cut(s) 77, 402, 787, 976, 1117
SsiI CCGC 5 cut(s) 89, 220, 461, 549, 615
SspDI GGCGCC 1 cut(s) 31
SspMI CTAG 4 cut(s) 47, 86, 756, 1162
SstI GAGCTC 1 cut(s) 609
StyD4I CCNGG 3 cut(s) 106, 530, 917
StyI CCWWGG 2 cut(s) 382, 755
TaaI ACNGT 3 cut(s) 137, 508, 851
TaiI ACGT 1 cut(s) 906
TaqI TCGA 5 cut(s) 13, 499, 609, 702, 1090
TasI AATT 5 cut(s) 77, 402, 787, 976, 1117
TatI WGTACW 1 cut(s) 697
TfiI GAWTC 4 cut(s) 53, 301, 496, 914
Tru1I TTAA 3 cut(s) 132, 252, 891
Tru9I TTAA 3 cut(s) 132, 252, 891
TscAI CASTG 4 cut(s) 298, 311, 978, 1090
TseFI GTSAC 1 cut(s) 983
TseI GCWGC 1 cut(s) 692
Tsp45I GTSAC 1 cut(s) 983
TspDTI ATGAA 3 cut(s) 498, 899, 1007
TspGWI ACGGA 1 cut(s) 737
TspRI CASTG 4 cut(s) 298, 311, 978, 1090
Van91I CCANNNNNTGG 1 cut(s) 932
Vha464I CTTAAG 1 cut(s) 890
VneI GTGCAC 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 472, 628, 1150
XagI CCTNNNNNAGG 1 cut(s) 636
XapI RAATTY 2 cut(s) 77, 976
XceI RCATGY 1 cut(s) 1100
XcmI CCANNNNNNNNNTGG 2 cut(s) 290, 661
XmaJI CCTAGG 1 cut(s) 755
XmiI GTMKAC 1 cut(s) 112
XspI CTAG 4 cut(s) 47, 86, 756, 1162
ZrmI AGTACT 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.