pycom05g26240

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011247
Physical Location & Seq
Forward (+)
27545747 .. 27547448
1702 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g26240.1

Sequence Viewer

Length: 1218 bp
ATGCAACGGGTTAGACTGTCCTCCCAACAGGCGCCGGTACACAAGCTAGGGGATTCTCAGATTGCGTTATCCCCGAAGTTTAGATTAGCGGTTATCCAGTCTTCGTTGTTGAATCCTTCCCCGGAGTTAGAGCTGTCTCTTGAGGAAACCCCCCTCATTCCTGGCCTTCCTGATGATGTTGCACTTAACTGTCTCCTTCGGGTTCCAGTCGAAGGCCACACAGCAAGCAAAGCTGTCTGCAAGCGTTGGCATCTAATGTTTGGTAGTAAAGAGCGGTTTTTTACTCGAAGAAAAGAATTAGGCTTCAAAGTCCCTTGGCTTTTTGTCTTTGCCTTTCACAAGTGCACTGGAAGGATTCAATGGCAGGTTCTCGATCTCACTAATTTCTCTTGGCACACTATCCCTGCCATGCCTTGCAGAGACAAAGTTTTTCCCCATGGATTCAGATGTGTATCGATGCCCCACGAAGGAACTCTCTTTATTTGCGGTGGTATGGTCTCTGATGTGGACTGTCCGCTTGACTTGGTCCTGAAATATGAAGTTCAGAAGAATCGGTGGACTGTGATAAATCAGATGATAAGTGCGAGGTCTTTTTTTGCAAGTGGACTTATTGATGGGAAGATTTATGTGGCTGGAGGAAACAGCTCAGATCTTTTTGAGCTTGACTCAGCCGAGGTTTTGGACCCCGTTAAAGGGAGTTGGCATCCCATAGCTAGCATGGGAGCAAATATGGCCTCTTATGATGCAGCAGTTCTCAACGGAAAGCTTCTTGTTACAGAAGGCTGGTTGTGGCCTTTCTTTGTCTCTCCCAGGGGTCAGGTTTATGATCCGAGAACTAATAATTGGGAGAGCATGGCTGTTGGACTGAGAGAAGGCTGGACAGGTTCAAGTGTGGTGATTTGTGGCCATTTGTTTGTGGTCTCAGAGCTTGAAAGGATGAAGCTTAAGGTTTATGATGCGGATACTGATTCCTGGGAACCAATAGAAGGGCCCCCTTTGCCAGAGCAGATCTGTAAACCCTTTGCTGTCAATGCCTGTGATTGCATGATATATGTTGTGGGCAAGAACCTTCATGTTGCTGTTGGTCAAATCTCAAGGGTTAGCCGTAAAGGAACTTGCGAGAAGAAGTGGAGCTTTGGTGTTCGATGGAATGTGGTTGATGCACCGGATTGTTTCTCTGACTTGACGCCCTCAAGTTCGCAGGTCCTGTTTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

406

Amino Acids

45.33

Weight (kDa)

7.11

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 122 - 287 1.5e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 122 - 217 7.6e-06 Attractin/LZTR1 beta-propeller
Beta-prop_Calicin PF13964 162 - 284 4.6e-07 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 173 - 335 3.1e-18 FKB95, Kelch-repeats domain
Kelch_1 PF01344 195 - 241 6.9e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 355, 1192
AccB1I GGYRCC 1 cut(s) 31
AccBSI CCGCTC 1 cut(s) 274
AciI CCGC 5 cut(s) 89, 274, 486, 515, 959
AclWI GGATC 1 cut(s) 821
AcoI YGGCCR 1 cut(s) 904
AcyI GRCGYC 2 cut(s) 32, 1187
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 5 cut(s) 212, 467, 692, 693, 986
AflII CTTAAG 1 cut(s) 944
AgsI TTSAA 5 cut(s) 112, 307, 359, 888, 932
AjnI CCWGG 3 cut(s) 160, 809, 971
AjuI GAANNNNNNNTTGG 2 cut(s) 826, 858
Alw21I GWGCWC 1 cut(s) 347
Alw26I GTCTC 6 cut(s) 141, 197, 414, 502, 808, 925
Alw44I GTGCAC 1 cut(s) 343
AlwI GGATC 1 cut(s) 821
AlwNI CAGNNNCTG 1 cut(s) 1207
AoxI GGCC 6 cut(s) 163, 214, 732, 791, 904, 989
ApaI GGGCCC 1 cut(s) 993
ApaLI GTGCAC 1 cut(s) 343
ApeKI GCWGC 1 cut(s) 746
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 5 cut(s) 526, 682, 989, 990, 1204
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 907
AsuNHI GCTAGC 1 cut(s) 713
AvaII GGWCC 3 cut(s) 526, 682, 1204
BaeGI GKGCMC 2 cut(s) 347, 993
BalI TGGCCA 1 cut(s) 906
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 347
BbvI GCAGC 1 cut(s) 758
BccI CCATC 2 cut(s) 608, 1140
BceAI ACGGC 1 cut(s) 1089
BciT130I CCWGG 3 cut(s) 162, 811, 973
BciVI GTATCC 1 cut(s) 955
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 6 cut(s) 141, 197, 414, 502, 808, 925
BfaI CTAG 2 cut(s) 47, 714
BfoI RGCGCY 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 944
BfuAI ACCTGC 2 cut(s) 355, 1192
BfuI GTATCC 1 cut(s) 955
BglII AGATCT 2 cut(s) 649, 1008
BisI GCNGC 1 cut(s) 747
BlsI GCNGC 1 cut(s) 748
Bme1390I CCNGG 4 cut(s) 122, 162, 811, 973
Bme18I GGWCC 3 cut(s) 526, 682, 1204
BmgT120I GGNCC 5 cut(s) 526, 682, 989, 990, 1204
BmiI GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 992
BmrFI CCNGG 4 cut(s) 122, 162, 811, 973
BmsI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
BmtI GCTAGC 1 cut(s) 717
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 1 cut(s) 654
BpuEI CTTGAG 3 cut(s) 161, 1078, 1177
BpuMI CCSGG 1 cut(s) 122
Bsa29I ATCGAT 1 cut(s) 455
BsaBI GATNNNNATC 1 cut(s) 451
BsaHI GRCGYC 2 cut(s) 32, 1187
BsaI GGTCTC 2 cut(s) 502, 925
BsaJI CCNNGG 7 cut(s) 120, 314, 436, 672, 809, 810, 972
BsaWI WCCGGW 1 cut(s) 1165
BsaXI ACNNNNNCTCC 2 cut(s) 1123, 1153
Bsc4I CCNNNNNNNGG 5 cut(s) 212, 467, 692, 693, 986
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 97, 206, 352
Bse8I GATNNNNATC 1 cut(s) 451
BseBI CCWGG 3 cut(s) 162, 811, 973
BseCI ATCGAT 1 cut(s) 455
BseDI CCNNGG 7 cut(s) 120, 314, 436, 672, 809, 810, 972
BseGI GGATG 2 cut(s) 703, 942
BseJI GATNNNNATC 1 cut(s) 451
BseLI CCNNNNNNNGG 5 cut(s) 212, 467, 692, 693, 986
BseMII CTCAG 5 cut(s) 71, 660, 681, 857, 936
BseNI ACTGG 3 cut(s) 97, 206, 352
BseSI GKGCMC 2 cut(s) 347, 993
BseXI GCAGC 1 cut(s) 758
BshFI GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
BshNI GGYRCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 347
BsiSI CCGG 3 cut(s) 35, 122, 1166
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 5 cut(s) 212, 467, 692, 693, 986
BsmAI GTCTC 6 cut(s) 141, 197, 414, 502, 808, 925
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
Bso31I GGTCTC 2 cut(s) 502, 925
Bsp120I GGGCCC 1 cut(s) 989
Bsp1286I GDGCHC 2 cut(s) 347, 993
Bsp143I GATC 4 cut(s) 373, 649, 826, 1008
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 5 cut(s) 89, 274, 486, 515, 959
BspANI GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
BspCNI CTCAG 5 cut(s) 70, 659, 680, 858, 935
BspDI ATCGAT 1 cut(s) 455
BspLI GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 992
BspMI ACCTGC 2 cut(s) 355, 1192
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 1 cut(s) 821
BspT107I GGYRCC 1 cut(s) 31
BspTI CTTAAG 1 cut(s) 944
BspTNI GGTCTC 2 cut(s) 502, 925
BsrBI CCGCTC 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 3 cut(s) 97, 206, 352
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 7 cut(s) 120, 314, 436, 672, 809, 810, 972
BssMI GATC 4 cut(s) 373, 649, 826, 1008
BssNI GRCGYC 2 cut(s) 32, 1187
BssT1I CCWWGG 2 cut(s) 314, 436
Bst2UI CCWGG 3 cut(s) 162, 811, 973
Bst4CI ACNGT 4 cut(s) 18, 191, 512, 562
BstACI GRCGYC 2 cut(s) 32, 1187
BstAFI CTTAAG 1 cut(s) 944
BstC8I GCNNGC 3 cut(s) 226, 242, 715
BstDEI CTNAG 5 cut(s) 57, 646, 667, 866, 922
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 2 cut(s) 703, 942
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 4 cut(s) 376, 652, 829, 1011
BstMAI GTCTC 6 cut(s) 141, 197, 414, 502, 808, 925
BstMBI GATC 4 cut(s) 373, 649, 826, 1008
BstMWI GCNNNNNNNGC 4 cut(s) 230, 731, 997, 1031
BstNI CCWGG 3 cut(s) 162, 811, 973
BstSCI CCNGG 4 cut(s) 120, 160, 809, 971
BstSLI GKGCMC 2 cut(s) 347, 993
BstV1I GCAGC 1 cut(s) 758
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 649, 1008
BstYI RGATCY 2 cut(s) 649, 1008
Bsu15I ATCGAT 1 cut(s) 455
BsuI GTATCC 1 cut(s) 955
BsuRI GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
BsuTUI ATCGAT 1 cut(s) 455
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 2 cut(s) 703, 942
BtsIMutI CAGTG 1 cut(s) 345
BveI ACCTGC 2 cut(s) 355, 1192
Cac8I GCNNGC 3 cut(s) 226, 242, 715
CaiI CAGNNNCTG 1 cut(s) 1207
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 5 cut(s) 526, 682, 989, 990, 1204
ClaI ATCGAT 1 cut(s) 455
CseI GACGC 1 cut(s) 1195
Csp6I GTAC 1 cut(s) 38
CviAII CATG 6 cut(s) 409, 437, 718, 853, 1045, 1073
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 5 cut(s) 57, 646, 667, 866, 922
DinI GGCGCC 1 cut(s) 33
DpnI GATC 4 cut(s) 375, 651, 828, 1010
DpnII GATC 4 cut(s) 373, 649, 826, 1008
EaeI YGGCCR 1 cut(s) 904
Eco130I CCWWGG 2 cut(s) 314, 436
Eco24I GRGCYC 1 cut(s) 993
Eco31I GGTCTC 2 cut(s) 502, 925
Eco47I GGWCC 3 cut(s) 526, 682, 1204
EcoO109I RGGNCCY 3 cut(s) 989, 990, 1204
EcoRII CCWGG 3 cut(s) 160, 809, 971
EcoT14I CCWWGG 2 cut(s) 314, 436
EcoT38I GRGCYC 1 cut(s) 993
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 314, 436
FaeI CATG 6 cut(s) 412, 440, 721, 856, 1048, 1076
FalI AAGNNNNNCTT 2 cut(s) 1118, 1150
FaqI GGGAC 1 cut(s) 296
FatI CATG 6 cut(s) 408, 436, 717, 852, 1044, 1072
Fnu4HI GCNGC 1 cut(s) 747
FokI GGATG 2 cut(s) 690, 949
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 1 cut(s) 747
FspBI CTAG 2 cut(s) 47, 714
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 747
GsuI CTGGAG 1 cut(s) 654
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
HapII CCGG 3 cut(s) 35, 122, 1166
HgaI GACGC 1 cut(s) 1195
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 2 cut(s) 32, 1187
Hin1II CATG 6 cut(s) 412, 440, 721, 856, 1048, 1076
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HindIII AAGCTT 2 cut(s) 764, 941
HinfI GANTC 7 cut(s) 53, 112, 355, 441, 550, 665, 968
HpaII CCGG 3 cut(s) 35, 122, 1166
HphI GGTGA 1 cut(s) 907
Hpy166II GTNNAC 6 cut(s) 40, 345, 508, 558, 605, 1016
Hpy188I TCNGA 9 cut(s) 60, 446, 502, 546, 573, 649, 831, 925, 1180
Hpy188III TCNNGA 4 cut(s) 140, 170, 371, 529
Hpy8I GTNNAC 6 cut(s) 40, 345, 508, 558, 605, 1016
HpyCH4III ACNGT 4 cut(s) 18, 191, 512, 562
HpyCH4V TGCA 9 cut(s) 4, 182, 240, 345, 417, 599, 746, 1044, 1163
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 731, 997, 1031
HpyF3I CTNAG 5 cut(s) 57, 646, 667, 866, 922
Hsp92I GRCGYC 2 cut(s) 32, 1187
Hsp92II CATG 6 cut(s) 412, 440, 721, 856, 1048, 1076
HspAI GCGC 1 cut(s) 32
KasI GGCGCC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 373, 649, 826, 1008
LmnI GCTCC 2 cut(s) 722, 1131
Lsp1109I GCAGC 1 cut(s) 758
LweI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
MaeI CTAG 2 cut(s) 47, 714
MaeIII GTNAC 1 cut(s) 772
MalI GATC 4 cut(s) 375, 651, 828, 1010
MbiI CCGCTC 1 cut(s) 274
MboI GATC 4 cut(s) 373, 649, 826, 1008
MboII GAAGA 5 cut(s) 93, 300, 559, 631, 1135
MflI RGATCY 2 cut(s) 649, 1008
MhlI GDGCHC 2 cut(s) 347, 993
MlsI TGGCCA 1 cut(s) 906
MluCI AATT 3 cut(s) 296, 382, 841
MluNI TGGCCA 1 cut(s) 906
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 1 cut(s) 841
MnlI CCTC 8 cut(s) 31, 136, 164, 579, 629, 667, 745, 1201
Mox20I TGGCCA 1 cut(s) 906
MscI TGGCCA 1 cut(s) 906
MseI TTAA 3 cut(s) 186, 690, 945
Msp20I TGGCCA 1 cut(s) 906
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 3 cut(s) 35, 122, 1166
MspR9I CCNGG 4 cut(s) 122, 162, 811, 973
MvaI CCWGG 3 cut(s) 162, 811, 973
MwoI GCNNNNNNNGC 4 cut(s) 230, 731, 997, 1031
NarI GGCGCC 1 cut(s) 32
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 436
NdeII GATC 4 cut(s) 373, 649, 826, 1008
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 6 cut(s) 412, 440, 721, 856, 1048, 1076
NlaIV GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 992
NmeAIII GCCGAG 1 cut(s) 697
PasI CCCWGGG 1 cut(s) 810
PfeI GAWTC 6 cut(s) 53, 112, 355, 441, 550, 968
PflFI GACNNNGTC 1 cut(s) 524
PkrI GCNGC 1 cut(s) 748
PleI GAGTC 1 cut(s) 659
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 659
PpuMI RGGWCCY 1 cut(s) 1204
Psp5II RGGWCCY 1 cut(s) 1204
Psp6I CCWGG 3 cut(s) 160, 809, 971
PspGI CCWGG 3 cut(s) 160, 809, 971
PspN4I GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 992
PspOMI GGGCCC 1 cut(s) 989
PspPI GGNCC 5 cut(s) 526, 682, 989, 990, 1204
PspPPI RGGWCCY 1 cut(s) 1204
PstNI CAGNNNCTG 1 cut(s) 1207
PsuI RGATCY 2 cut(s) 649, 1008
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
SaqAI TTAA 3 cut(s) 186, 690, 945
SatI GCNGC 1 cut(s) 747
Sau3AI GATC 4 cut(s) 373, 649, 826, 1008
Sau96I GGNCC 5 cut(s) 526, 682, 989, 990, 1204
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 4 cut(s) 122, 162, 811, 973
SduI GDGCHC 2 cut(s) 347, 993
SfaNI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
SfoI GGCGCC 1 cut(s) 33
SinI GGWCC 3 cut(s) 526, 682, 1204
SmlI CTYRAG 4 cut(s) 140, 944, 1093, 1192
SmoI CTYRAG 4 cut(s) 140, 944, 1093, 1192
Sse9I AATT 3 cut(s) 296, 382, 841
SsiI CCGC 5 cut(s) 89, 274, 486, 515, 959
SspDI GGCGCC 1 cut(s) 31
SspMI CTAG 2 cut(s) 47, 714
StyD4I CCNGG 4 cut(s) 120, 160, 809, 971
StyI CCWWGG 2 cut(s) 314, 436
TaaI ACNGT 4 cut(s) 18, 191, 512, 562
TaqI TCGA 5 cut(s) 210, 286, 372, 455, 1144
TasI AATT 3 cut(s) 296, 382, 841
TfiI GAWTC 6 cut(s) 53, 112, 355, 441, 550, 968
Tru1I TTAA 3 cut(s) 186, 690, 945
Tru9I TTAA 3 cut(s) 186, 690, 945
TscAI CASTG 1 cut(s) 352
TseI GCWGC 1 cut(s) 746
TspDTI ATGAA 3 cut(s) 552, 953, 1061
TspGWI ACGGA 1 cut(s) 774
TspRI CASTG 1 cut(s) 352
Tth111I GACNNNGTC 1 cut(s) 524
Vha464I CTTAAG 1 cut(s) 944
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 3 cut(s) 526, 682, 1204
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XspI CTAG 2 cut(s) 47, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.