MD05G1283000.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011247
Physical Location & Seq
Forward (+)
41658344 .. 41660204
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1283000.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGCAACGGGTTAGACTGTCCTCCCAACAGGCGCCGGTACACAAGCTGGGGGATTCTCAAATTGCATTATCCCCGAAGTTTAGATTAGCGGTTATCCAGTCTTCGTTGTTGAATCCTTCCCCGGAGTTAGAGCTGTCTCTTGAGGAAACCCCCCTCATTCCTGGCCTTCCTGATGATGTTGCACTTAACTGTCTCCTTCGGGTTCCAGTCGAAGGCCACACAGCAAGCAAGGCTGTCTGCAAGCGTTGGCATCTAATGTTTGGTAGTAAAGAGCGGTTTTTTACTCGAAGAAAAGAACTAGGCTTCAAAGTCCCTTGGCTTTTTGTCTTTGCCTTTCACAAGTGCACTGGAAGGATTCAATGGCAGGTTCTTGATCTCACTAATTTCTCTTGGCACACTATCCCTGCCATGCCTTGCAGAGACAAAGTTTTTCCCCATGGATTCAGATGTGTATCGATGCCCCAAGAAGGAACTCTCTTTATTTGTGGTGGTATGGTCTCTGACGTGGACTGTCCGCTTGACTTGGTCCTGAAATATGAAGTTCAGAAGAATCGGTGGACTGTGATAAATCAGATGATAAGTGCGAGGTCTTTTTTTGCAAGTGGAGTTATTGATGGGAAGATTTATGTGGCTGGAGGAAACAGCTCAGATCTTTTTGAGCTTGACTCAGCCGAGGTTTTGGACCCCGTTAAAGGGAGTTGGCATCCCATAGCTAGCATGGGAGCAAATATGGCCTCTTATGATGCAGCAGTTCTCAACGGAAAGCTTCTTGTTACAGAAGGCTGGTTGTGGCCTTTCTTTGTCTCTCCCAGGGGTCAGGTTTATGATCCGAGAACTAATAATTGGGAGAGCATGGCTGTCGGACTGAGAGAAGGCTGGACAGGTTCAAGTGTGGTGATTTATGGCCATTTGTTTGTGGTCTCGGAGCTTGAAAGGATGAAGCTTAAGGTTTATGATGCCGATACTGATTCCTGGGAACCAATAGAAGGGCCCCCTTTGCCAGAGCAGATCTGTAAACCCTTTGCTGTCAATGCCTGTGATTGCATGATATATGTTGTGGGCAAGAACCTTCATGTTGCTGTGGGTCAAATCTCAAGGGTTAGCCGTAAAGGAACTTGCGAGAAGAAGTGGAGCTTTGGTGTTCGATGGAATGTGGTTGATGCACCGGATTGTTTCTCCGACTTGACGCCCTCAAGTTCGCAGGTCCTGTTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

406

Amino Acids

45.29

Weight (kDa)

7.08

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 121 - 217 6.8e-06 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 122 - 287 1.1e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 162 - 284 2.6e-07 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 173 - 350 1e-18 FKB95, Kelch-repeats domain
Kelch_1 PF01344 195 - 241 5.8e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 355, 1192
AccB1I GGYRCC 1 cut(s) 31
AccBSI CCGCTC 1 cut(s) 274
AciI CCGC 3 cut(s) 89, 274, 515
AclWI GGATC 1 cut(s) 821
AcoI YGGCCR 1 cut(s) 904
AcyI GRCGYC 2 cut(s) 32, 1187
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 5 cut(s) 212, 467, 692, 693, 986
AflII CTTAAG 1 cut(s) 944
AgsI TTSAA 5 cut(s) 112, 307, 359, 888, 932
AjiI CACGTC 1 cut(s) 505
AjnI CCWGG 3 cut(s) 160, 809, 971
AjuI GAANNNNNNNTTGG 2 cut(s) 826, 858
AluBI AGCT 9 cut(s) 46, 133, 645, 661, 713, 766, 928, 943, 1134
AluI AGCT 9 cut(s) 46, 133, 645, 661, 713, 766, 928, 943, 1134
Alw21I GWGCWC 1 cut(s) 347
Alw26I GTCTC 6 cut(s) 141, 197, 414, 502, 808, 925
Alw44I GTGCAC 1 cut(s) 343
AlwI GGATC 1 cut(s) 821
AlwNI CAGNNNCTG 1 cut(s) 1207
AoxI GGCC 6 cut(s) 163, 214, 732, 791, 904, 989
ApaI GGGCCC 1 cut(s) 993
ApaLI GTGCAC 1 cut(s) 343
ApeKI GCWGC 1 cut(s) 746
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 5 cut(s) 526, 682, 989, 990, 1204
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 907
AsuNHI GCTAGC 1 cut(s) 713
AvaII GGWCC 3 cut(s) 526, 682, 1204
BaeGI GKGCMC 2 cut(s) 347, 993
BalI TGGCCA 1 cut(s) 906
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 347
BbvI GCAGC 1 cut(s) 758
BccI CCATC 2 cut(s) 608, 1140
BceAI ACGGC 1 cut(s) 1089
BcgI CGANNNNNNTGC 2 cut(s) 841, 875
BciT130I CCWGG 3 cut(s) 162, 811, 973
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 6 cut(s) 141, 197, 414, 502, 808, 925
BfaI CTAG 2 cut(s) 299, 714
BfoI RGCGCY 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 944
BfuAI ACCTGC 2 cut(s) 355, 1192
BglII AGATCT 2 cut(s) 649, 1008
BisI GCNGC 1 cut(s) 747
BlsI GCNGC 1 cut(s) 748
Bme1390I CCNGG 4 cut(s) 122, 162, 811, 973
Bme18I GGWCC 3 cut(s) 526, 682, 1204
BmgBI CACGTC 1 cut(s) 505
BmgT120I GGNCC 5 cut(s) 526, 682, 989, 990, 1204
BmiI GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 992
BmrFI CCNGG 4 cut(s) 122, 162, 811, 973
BmsI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
BmtI GCTAGC 1 cut(s) 717
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 1 cut(s) 654
BpuEI CTTGAG 3 cut(s) 161, 1078, 1177
BpuMI CCSGG 1 cut(s) 122
Bsa29I ATCGAT 1 cut(s) 455
BsaBI GATNNNNATC 1 cut(s) 451
BsaHI GRCGYC 2 cut(s) 32, 1187
BsaI GGTCTC 2 cut(s) 502, 925
BsaJI CCNNGG 7 cut(s) 120, 314, 436, 672, 809, 810, 972
BsaWI WCCGGW 1 cut(s) 1165
BsaXI ACNNNNNCTCC 2 cut(s) 1123, 1153
Bsc4I CCNNNNNNNGG 5 cut(s) 212, 467, 692, 693, 986
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 97, 206, 352
Bse8I GATNNNNATC 1 cut(s) 451
BseBI CCWGG 3 cut(s) 162, 811, 973
BseCI ATCGAT 1 cut(s) 455
BseDI CCNNGG 7 cut(s) 120, 314, 436, 672, 809, 810, 972
BseGI GGATG 2 cut(s) 703, 942
BseJI GATNNNNATC 1 cut(s) 451
BseLI CCNNNNNNNGG 5 cut(s) 212, 467, 692, 693, 986
BseMII CTCAG 3 cut(s) 660, 681, 857
BseNI ACTGG 3 cut(s) 97, 206, 352
BseSI GKGCMC 2 cut(s) 347, 993
BseXI GCAGC 1 cut(s) 758
BseYI CCCAGC 1 cut(s) 46
BshFI GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
BshNI GGYRCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 347
BsiSI CCGG 3 cut(s) 35, 122, 1166
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 5 cut(s) 212, 467, 692, 693, 986
BsmAI GTCTC 6 cut(s) 141, 197, 414, 502, 808, 925
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
Bso31I GGTCTC 2 cut(s) 502, 925
Bsp120I GGGCCC 1 cut(s) 989
Bsp1286I GDGCHC 2 cut(s) 347, 993
Bsp143I GATC 4 cut(s) 373, 649, 826, 1008
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 3 cut(s) 89, 274, 515
BspANI GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
BspCNI CTCAG 3 cut(s) 659, 680, 858
BspDI ATCGAT 1 cut(s) 455
BspLI GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 992
BspMI ACCTGC 2 cut(s) 355, 1192
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 1 cut(s) 821
BspT107I GGYRCC 1 cut(s) 31
BspTI CTTAAG 1 cut(s) 944
BspTNI GGTCTC 2 cut(s) 502, 925
BsrBI CCGCTC 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 3 cut(s) 97, 206, 352
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 7 cut(s) 120, 314, 436, 672, 809, 810, 972
BssMI GATC 4 cut(s) 373, 649, 826, 1008
BssNI GRCGYC 2 cut(s) 32, 1187
BssT1I CCWWGG 2 cut(s) 314, 436
Bst2UI CCWGG 3 cut(s) 162, 811, 973
Bst4CI ACNGT 4 cut(s) 18, 191, 512, 562
BstACI GRCGYC 2 cut(s) 32, 1187
BstAFI CTTAAG 1 cut(s) 944
BstC8I GCNNGC 3 cut(s) 226, 242, 715
BstDEI CTNAG 4 cut(s) 646, 667, 866, 1215
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 2 cut(s) 703, 942
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 4 cut(s) 376, 652, 829, 1011
BstMAI GTCTC 6 cut(s) 141, 197, 414, 502, 808, 925
BstMBI GATC 4 cut(s) 373, 649, 826, 1008
BstMWI GCNNNNNNNGC 4 cut(s) 230, 731, 997, 1031
BstNI CCWGG 3 cut(s) 162, 811, 973
BstSCI CCNGG 4 cut(s) 120, 160, 809, 971
BstSLI GKGCMC 2 cut(s) 347, 993
BstV1I GCAGC 1 cut(s) 758
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 649, 1008
BstYI RGATCY 2 cut(s) 649, 1008
Bsu15I ATCGAT 1 cut(s) 455
BsuRI GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
BsuTUI ATCGAT 1 cut(s) 455
BtgI CCRYGG 1 cut(s) 436
BtrI CACGTC 1 cut(s) 505
BtsCI GGATG 2 cut(s) 703, 942
BtsIMutI CAGTG 1 cut(s) 345
BveI ACCTGC 2 cut(s) 355, 1192
Cac8I GCNNGC 3 cut(s) 226, 242, 715
CaiI CAGNNNCTG 1 cut(s) 1207
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 5 cut(s) 526, 682, 989, 990, 1204
ClaI ATCGAT 1 cut(s) 455
CseI GACGC 1 cut(s) 1195
Csp6I GTAC 1 cut(s) 38
CviAII CATG 6 cut(s) 409, 437, 718, 853, 1045, 1073
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 4 cut(s) 646, 667, 866, 1215
DinI GGCGCC 1 cut(s) 33
DpnI GATC 4 cut(s) 375, 651, 828, 1010
DpnII GATC 4 cut(s) 373, 649, 826, 1008
EaeI YGGCCR 1 cut(s) 904
Eco130I CCWWGG 2 cut(s) 314, 436
Eco24I GRGCYC 1 cut(s) 993
Eco31I GGTCTC 2 cut(s) 502, 925
Eco47I GGWCC 3 cut(s) 526, 682, 1204
EcoO109I RGGNCCY 3 cut(s) 989, 990, 1204
EcoRII CCWGG 3 cut(s) 160, 809, 971
EcoT14I CCWWGG 2 cut(s) 314, 436
EcoT38I GRGCYC 1 cut(s) 993
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 314, 436
FaeI CATG 6 cut(s) 412, 440, 721, 856, 1048, 1076
FalI AAGNNNNNCTT 2 cut(s) 1118, 1150
FaqI GGGAC 1 cut(s) 296
FatI CATG 6 cut(s) 408, 436, 717, 852, 1044, 1072
Fnu4HI GCNGC 1 cut(s) 747
FokI GGATG 2 cut(s) 690, 949
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 1 cut(s) 747
FspBI CTAG 2 cut(s) 299, 714
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 747
GsaI CCCAGC 1 cut(s) 50
GsuI CTGGAG 1 cut(s) 654
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 6 cut(s) 165, 216, 734, 793, 906, 991
HapII CCGG 3 cut(s) 35, 122, 1166
HgaI GACGC 1 cut(s) 1195
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 2 cut(s) 32, 1187
Hin1II CATG 6 cut(s) 412, 440, 721, 856, 1048, 1076
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HindIII AAGCTT 2 cut(s) 764, 941
HinfI GANTC 7 cut(s) 53, 112, 355, 441, 550, 665, 968
HpaII CCGG 3 cut(s) 35, 122, 1166
HphI GGTGA 1 cut(s) 907
Hpy166II GTNNAC 5 cut(s) 40, 345, 508, 558, 1016
Hpy188I TCNGA 9 cut(s) 446, 502, 546, 573, 649, 831, 863, 925, 1180
Hpy188III TCNNGA 4 cut(s) 140, 170, 371, 529
Hpy8I GTNNAC 5 cut(s) 40, 345, 508, 558, 1016
HpyCH4III ACNGT 4 cut(s) 18, 191, 512, 562
HpyCH4IV ACGT 1 cut(s) 504
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 731, 997, 1031
HpyF3I CTNAG 4 cut(s) 646, 667, 866, 1215
HpySE526I ACGT 1 cut(s) 504
Hsp92I GRCGYC 2 cut(s) 32, 1187
Hsp92II CATG 6 cut(s) 412, 440, 721, 856, 1048, 1076
HspAI GCGC 1 cut(s) 32
KasI GGCGCC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 373, 649, 826, 1008
LmnI GCTCC 3 cut(s) 722, 925, 1131
Lsp1109I GCAGC 1 cut(s) 758
LweI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
MaeI CTAG 2 cut(s) 299, 714
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 1 cut(s) 772
MalI GATC 4 cut(s) 375, 651, 828, 1010
MbiI CCGCTC 1 cut(s) 274
MboI GATC 4 cut(s) 373, 649, 826, 1008
MboII GAAGA 5 cut(s) 93, 300, 559, 631, 1135
MflI RGATCY 2 cut(s) 649, 1008
MhlI GDGCHC 2 cut(s) 347, 993
MlsI TGGCCA 1 cut(s) 906
MluCI AATT 3 cut(s) 60, 382, 841
MluNI TGGCCA 1 cut(s) 906
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 2 cut(s) 841, 1203
MnlI CCTC 8 cut(s) 31, 136, 164, 579, 629, 667, 745, 1201
Mox20I TGGCCA 1 cut(s) 906
MscI TGGCCA 1 cut(s) 906
MseI TTAA 3 cut(s) 186, 690, 945
Msp20I TGGCCA 1 cut(s) 906
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 3 cut(s) 35, 122, 1166
MspR9I CCNGG 4 cut(s) 122, 162, 811, 973
MvaI CCWGG 3 cut(s) 162, 811, 973
MwoI GCNNNNNNNGC 4 cut(s) 230, 731, 997, 1031
NarI GGCGCC 1 cut(s) 32
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 436
NdeII GATC 4 cut(s) 373, 649, 826, 1008
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 6 cut(s) 412, 440, 721, 856, 1048, 1076
NlaIV GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 992
NmeAIII GCCGAG 1 cut(s) 697
PasI CCCWGGG 1 cut(s) 810
PfeI GAWTC 6 cut(s) 53, 112, 355, 441, 550, 968
PflFI GACNNNGTC 1 cut(s) 524
PkrI GCNGC 1 cut(s) 748
PleI GAGTC 1 cut(s) 659
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 659
PpuMI RGGWCCY 1 cut(s) 1204
Psp5II RGGWCCY 1 cut(s) 1204
Psp6I CCWGG 3 cut(s) 160, 809, 971
PspFI CCCAGC 1 cut(s) 46
PspGI CCWGG 3 cut(s) 160, 809, 971
PspN4I GGNNCC 6 cut(s) 33, 204, 684, 978, 991, 992
PspOMI GGGCCC 1 cut(s) 989
PspPI GGNCC 5 cut(s) 526, 682, 989, 990, 1204
PspPPI RGGWCCY 1 cut(s) 1204
PstNI CAGNNNCTG 1 cut(s) 1207
PsuI RGATCY 2 cut(s) 649, 1008
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
SaqAI TTAA 3 cut(s) 186, 690, 945
SatI GCNGC 1 cut(s) 747
Sau3AI GATC 4 cut(s) 373, 649, 826, 1008
Sau96I GGNCC 5 cut(s) 526, 682, 989, 990, 1204
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 4 cut(s) 122, 162, 811, 973
SduI GDGCHC 2 cut(s) 347, 993
SfaNI GCATC 6 cut(s) 259, 447, 712, 733, 946, 1150
SfoI GGCGCC 1 cut(s) 33
SinI GGWCC 3 cut(s) 526, 682, 1204
SmlI CTYRAG 4 cut(s) 140, 944, 1093, 1192
SmoI CTYRAG 4 cut(s) 140, 944, 1093, 1192
Sse9I AATT 3 cut(s) 60, 382, 841
SsiI CCGC 3 cut(s) 89, 274, 515
SspDI GGCGCC 1 cut(s) 31
SspMI CTAG 2 cut(s) 299, 714
StyD4I CCNGG 4 cut(s) 120, 160, 809, 971
StyI CCWWGG 2 cut(s) 314, 436
TaaI ACNGT 4 cut(s) 18, 191, 512, 562
TaiI ACGT 1 cut(s) 507
TaqI TCGA 4 cut(s) 210, 286, 455, 1144
TasI AATT 3 cut(s) 60, 382, 841
TfiI GAWTC 6 cut(s) 53, 112, 355, 441, 550, 968
Tru1I TTAA 3 cut(s) 186, 690, 945
Tru9I TTAA 3 cut(s) 186, 690, 945
TscAI CASTG 1 cut(s) 352
TseI GCWGC 1 cut(s) 746
TspDTI ATGAA 3 cut(s) 552, 953, 1061
TspGWI ACGGA 1 cut(s) 774
TspRI CASTG 1 cut(s) 352
Tth111I GACNNNGTC 1 cut(s) 524
Vha464I CTTAAG 1 cut(s) 944
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 3 cut(s) 526, 682, 1204
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XspI CTAG 2 cut(s) 299, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.