FvH4_3g05601

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018392
Physical Location & Seq
Reverse (-)
3279380 .. 3280279
900 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05601.t1

Sequence Viewer

Length: 900 bp
ATGTGTACCGAGAGACGTCGCCACTTTCTTAGTGAGGCGCGGCTGGAGAAGTCGATTGAATTCTTGGATGAAACCCGAAGGGGAATGGTTCTGATCAGATTGCAGCTCTACAAGCTTACCGGGAGTGGTTGGATGGATGGAGACGCCTATGAAGAATTTCCAGTTGCAAGAGATGTTACTTATGAAGAATCTTCCATCTGGACAGAATCTCACTTTTGGATCAGCCTCTCATCTCTCAGGCACCTGCCGACAAGGAAGAAGCACACTGAGCTTTTCACAGAGGTTCTAGAGGATTGGAATGTGCCAAAACCTCAGCAGCCGGCTATTATTGCACAATTGTTTCAAGCGGTTGATGGAGCTGCCCCCAAGTTGTCTATCATCGTCGCCGTACGCAGCTTCAATCTGCGGTTGCGCACAGTGAGTAGCTTCGGTTATGAAGCGACAGAGCTCAATCCTACATTCGCTGAGGAGAGTTTAAAGAAAGTGGGACTTGATAGCTCCGGAGCTACAGAATACAAGACAACCCGTTCATGTGTTTTGTGCATGGAGGGCTTTTACGAATCTGACAGTGCTGTTCAACAACTTACTCGCTTGCCCTGCTTGCACTGTTATCATGAACACTGCTTACTTAAGTGGCCATCGGAGATAAGACGCTCGTGCCCCTTGTGCGTAAAGCCACAGTCGAAATTTCATGCTTGGCGGCAATTTTGGGTTGCCATGGTGAGGAAGCACGCGGGTACTGTAATATCTGCTCTGATTTTTTGCCAATTTTTCAAGCAAGTCCAGCCTTCGATTCAAGTCCAGCCTTTAAAGACCACACCTGCACATCGACCTAGAAGTTCAGAAGTTGGAGCCAGAATGCAGAGTCTGCTTGAATTGGAATATTGGATGAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

300

Amino Acids

34.62

Weight (kDa)

8.53

Isoelectric Point (pI)

60.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 177 - 223 1.1e-08 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018392)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05601
rosa_chinensis RchiOBHm_Chr5g0014071
rosa_laevigata RLG00000032079
rosa_multiflora Rmu_sc0007767.1_g000009
rosa_rugosa Rorug05G0015300 Rorug05G0015400
rosa_samantha Rh5AG109400 Rh5BG106200 Rh5DG104900
rosa_wichuraiana Rw5G009550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 252, 829
AatII GACGTC 1 cut(s) 19
Acc16I TGCGCA 1 cut(s) 413
Acc36I ACCTGC 2 cut(s) 252, 829
AccB1I GGYRCC 1 cut(s) 240
AccII CGCG 2 cut(s) 40, 734
AccIII TCCGGA 1 cut(s) 500
AciI CCGC 5 cut(s) 40, 347, 406, 700, 734
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 1 cut(s) 635
AcsI RAATTY 3 cut(s) 59, 155, 686
AcyI GRCGYC 2 cut(s) 16, 144
AfaI GTAC 3 cut(s) 7, 390, 739
AfiI CCNNNNNNNGG 1 cut(s) 723
AflII CTTAAG 1 cut(s) 629
AgsI TTSAA 7 cut(s) 59, 344, 400, 578, 775, 797, 875
AluBI AGCT 9 cut(s) 106, 115, 271, 359, 396, 426, 448, 498, 506
AluI AGCT 9 cut(s) 106, 115, 271, 359, 396, 426, 448, 498, 506
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 7, 135
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 868
Aor13HI TCCGGA 1 cut(s) 500
AoxI GGCC 1 cut(s) 635
ApeKI GCWGC 4 cut(s) 103, 316, 359, 393
ApoI RAATTY 3 cut(s) 59, 155, 686
Asp700I GAANNNNTTC 1 cut(s) 156
AspLEI GCGC 2 cut(s) 40, 414
AsuC2I CCSGG 1 cut(s) 121
AsuHPI GGTGA 1 cut(s) 733
BaeGI GKGCMC 1 cut(s) 662
BalI TGGCCA 1 cut(s) 637
BanI GGYRCC 1 cut(s) 240
BanII GRGCYC 1 cut(s) 450
BauI CACGAG 1 cut(s) 655
Bbv12I GWGCWC 1 cut(s) 450
BbvCI CCTCAGC 2 cut(s) 312, 465
BbvI GCAGC 4 cut(s) 115, 328, 346, 405
BccI CCATC 5 cut(s) 127, 131, 203, 347, 646
BceAI ACGGC 1 cut(s) 371
BclI TGATCA 1 cut(s) 93
BcnI CCSGG 1 cut(s) 121
BcoDI GTCTC 2 cut(s) 7, 135
BfaI CTAG 2 cut(s) 287, 834
BfmI CTRYAG 1 cut(s) 507
BfrI CTTAAG 1 cut(s) 629
BfuAI ACCTGC 2 cut(s) 252, 829
BisI GCNGC 6 cut(s) 41, 104, 317, 360, 394, 701
BlsI GCNGC 6 cut(s) 42, 105, 318, 361, 395, 702
Bme1390I CCNGG 1 cut(s) 121
BmiI GGNNCC 2 cut(s) 242, 853
BmrFI CCNGG 1 cut(s) 121
BpmI CTGGAG 1 cut(s) 65
Bpu10I CCTNAGC 2 cut(s) 312, 465
BpuMI CCSGG 1 cut(s) 121
BsaHI GRCGYC 2 cut(s) 16, 144
BsaJI CCNNGG 1 cut(s) 717
BsaWI WCCGGW 1 cut(s) 500
Bsc4I CCNNNNNNNGG 1 cut(s) 723
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 161
BseAI TCCGGA 1 cut(s) 500
BseDI CCNNGG 1 cut(s) 717
BseGI GGATG 4 cut(s) 73, 138, 142, 894
BseLI CCNNNNNNNGG 1 cut(s) 723
BseMII CTCAG 4 cut(s) 250, 258, 326, 456
BseNI ACTGG 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 482
BseSI GKGCMC 1 cut(s) 662
BseXI GCAGC 4 cut(s) 115, 328, 346, 405
BsgI GTGCAG 1 cut(s) 807
Bsh1236I CGCG 2 cut(s) 40, 734
BshFI GGCC 1 cut(s) 637
BshNI GGYRCC 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 3 cut(s) 120, 320, 501
BsiWI CGTACG 1 cut(s) 388
BslFI GGGAC 1 cut(s) 501
BslI CCNNNNNNNGG 1 cut(s) 723
BsmAI GTCTC 2 cut(s) 7, 135
BsmBI CGTCTC 2 cut(s) 7, 135
BsmFI GGGAC 1 cut(s) 501
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 1 cut(s) 637
Bsp1286I GDGCHC 2 cut(s) 450, 662
Bsp13I TCCGGA 1 cut(s) 500
Bsp143I GATC 2 cut(s) 93, 219
Bsp19I CCATGG 1 cut(s) 717
BspACI CCGC 5 cut(s) 40, 347, 406, 700, 734
BspANI GGCC 1 cut(s) 637
BspCNI CTCAG 4 cut(s) 249, 259, 325, 457
BspEI TCCGGA 1 cut(s) 500
BspFNI CGCG 2 cut(s) 40, 734
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 2 cut(s) 242, 853
BspMI ACCTGC 2 cut(s) 252, 829
BspPI GGATC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 629
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 717
BssMI GATC 2 cut(s) 93, 219
BssNI GRCGYC 2 cut(s) 16, 144
BssSI CACGAG 1 cut(s) 655
BssT1I CCWWGG 1 cut(s) 717
Bst2BI CACGAG 1 cut(s) 655
Bst4CI ACNGT 5 cut(s) 418, 569, 608, 681, 742
BstACI GRCGYC 2 cut(s) 16, 144
BstAFI CTTAAG 1 cut(s) 629
BstAPI GCANNNNNTGC 1 cut(s) 868
BstC8I GCNNGC 4 cut(s) 321, 593, 602, 732
BstDEI CTNAG 5 cut(s) 29, 236, 267, 312, 465
BstDSI CCRYGG 1 cut(s) 717
BstF5I GGATG 4 cut(s) 73, 138, 142, 894
BstFNI CGCG 2 cut(s) 40, 734
BstHHI GCGC 2 cut(s) 40, 414
BstKTI GATC 2 cut(s) 96, 222
BstMAI GTCTC 2 cut(s) 7, 135
BstMBI GATC 2 cut(s) 93, 219
BstMWI GCNNNNNNNGC 9 cut(s) 112, 268, 329, 549, 597, 601, 666, 784, 868
BstSCI CCNGG 1 cut(s) 119
BstSFI CTRYAG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 662
BstUI CGCG 2 cut(s) 40, 734
BstV1I GCAGC 4 cut(s) 115, 328, 346, 405
BsuRI GGCC 1 cut(s) 637
BtgI CCRYGG 1 cut(s) 717
BtsCI GGATG 4 cut(s) 73, 138, 142, 894
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 5 cut(s) 264, 423, 574, 604, 619
BveI ACCTGC 2 cut(s) 252, 829
Cac8I GCNNGC 4 cut(s) 321, 593, 602, 732
CaiI CAGNNNCTG 1 cut(s) 868
CciI TCATGA 1 cut(s) 613
CfoI GCGC 2 cut(s) 40, 414
Cfr10I RCCGGY 1 cut(s) 319
CseI GACGC 2 cut(s) 152, 660
Csp6I GTAC 3 cut(s) 6, 389, 738
CviAII CATG 5 cut(s) 531, 544, 614, 692, 718
CviQI GTAC 3 cut(s) 6, 389, 738
DdeI CTNAG 5 cut(s) 29, 236, 267, 312, 465
DpnI GATC 2 cut(s) 95, 221
DpnII GATC 2 cut(s) 93, 219
DraI TTTAAA 2 cut(s) 477, 810
EaeI YGGCCR 1 cut(s) 635
Ecl136II GAGCTC 1 cut(s) 448
Eco130I CCWWGG 1 cut(s) 717
Eco24I GRGCYC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 448
EcoICRI GAGCTC 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 717
EcoT38I GRGCYC 1 cut(s) 450
ErhI CCWWGG 1 cut(s) 717
Esp3I CGTCTC 2 cut(s) 7, 135
FaeI CATG 5 cut(s) 534, 547, 617, 695, 721
FaiI YATR 8 cut(s) 150, 183, 435, 532, 545, 615, 693, 719
FalI AAGNNNNNCTT 2 cut(s) 474, 506
FaqI GGGAC 1 cut(s) 501
FatI CATG 5 cut(s) 530, 543, 613, 691, 717
FauI CCCGC 1 cut(s) 727
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 6 cut(s) 41, 104, 317, 360, 394, 701
FokI GGATG 3 cut(s) 80, 145, 149
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 6 cut(s) 41, 104, 317, 360, 394, 701
FspBI CTAG 2 cut(s) 287, 834
FspI TGCGCA 1 cut(s) 413
GlaI GCGC 2 cut(s) 39, 413
GluI GCNGC 6 cut(s) 41, 104, 317, 360, 394, 701
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 1 cut(s) 637
HapII CCGG 3 cut(s) 120, 320, 501
HgaI GACGC 2 cut(s) 152, 660
HhaI GCGC 2 cut(s) 40, 414
Hin1I GRCGYC 2 cut(s) 16, 144
Hin1II CATG 5 cut(s) 534, 547, 617, 695, 721
Hin6I GCGC 2 cut(s) 38, 412
HinP1I GCGC 2 cut(s) 38, 412
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 5 cut(s) 188, 206, 560, 793, 865
HpaII CCGG 3 cut(s) 120, 320, 501
HphI GGTGA 1 cut(s) 733
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 6 cut(s) 93, 98, 565, 643, 756, 844
Hpy188III TCNNGA 4 cut(s) 199, 287, 501, 614
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 21, 386
HpyAV CCTTC 2 cut(s) 72, 798
HpyCH4III ACNGT 5 cut(s) 418, 569, 608, 681, 742
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 7 cut(s) 103, 167, 332, 543, 604, 824, 862
HpyF10VI GCNNNNNNNGC 9 cut(s) 112, 268, 329, 549, 597, 601, 666, 784, 868
HpyF3I CTNAG 5 cut(s) 29, 236, 267, 312, 465
HpySE526I ACGT 1 cut(s) 16
Hsp92I GRCGYC 2 cut(s) 16, 144
Hsp92II CATG 5 cut(s) 534, 547, 617, 695, 721
HspAI GCGC 2 cut(s) 38, 412
Kpn2I TCCGGA 1 cut(s) 500
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 2 cut(s) 93, 219
LmnI GCTCC 4 cut(s) 356, 503, 503, 851
Lsp1109I GCAGC 4 cut(s) 115, 328, 346, 405
MaeI CTAG 2 cut(s) 287, 834
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 175
MalI GATC 2 cut(s) 95, 221
MboI GATC 2 cut(s) 93, 219
MboII GAAGA 4 cut(s) 164, 183, 197, 268
MfeI CAATTG 1 cut(s) 335
MhlI GDGCHC 2 cut(s) 450, 662
MlsI TGGCCA 1 cut(s) 637
MluCI AATT 7 cut(s) 59, 155, 335, 686, 704, 767, 875
MluNI TGGCCA 1 cut(s) 637
MlyI GAGTC 1 cut(s) 874
MmeI TCCRAC 2 cut(s) 110, 829
MnlI CCTC 8 cut(s) 28, 236, 274, 283, 321, 460, 541, 717
Mox20I TGGCCA 1 cut(s) 637
MroI TCCGGA 1 cut(s) 500
MroNI GCCGGC 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 156
MscI TGGCCA 1 cut(s) 637
MseI TTAA 3 cut(s) 476, 630, 809
Msp20I TGGCCA 1 cut(s) 637
MspCI CTTAAG 1 cut(s) 629
MspI CCGG 3 cut(s) 120, 320, 501
MspR9I CCNGG 1 cut(s) 121
MunI CAATTG 1 cut(s) 335
Mva1269I GAATGC 1 cut(s) 864
MvnI CGCG 2 cut(s) 40, 734
MwoI GCNNNNNNNGC 9 cut(s) 112, 268, 329, 549, 597, 601, 666, 784, 868
NaeI GCCGGC 1 cut(s) 321
NciI CCSGG 1 cut(s) 121
NcoI CCATGG 1 cut(s) 717
NdeII GATC 2 cut(s) 93, 219
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 5 cut(s) 534, 547, 617, 695, 721
NlaIV GGNNCC 2 cut(s) 242, 853
NsbI TGCGCA 1 cut(s) 413
PagI TCATGA 1 cut(s) 613
PaqCI CACCTGC 2 cut(s) 252, 829
PctI GAATGC 1 cut(s) 864
PdiI GCCGGC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 4 cut(s) 188, 206, 560, 793
Pfl23II CGTACG 1 cut(s) 388
PkrI GCNGC 6 cut(s) 42, 105, 318, 361, 395, 702
PleI GAGTC 1 cut(s) 873
PpsI GAGTC 1 cut(s) 873
Psp124BI GAGCTC 1 cut(s) 450
PspLI CGTACG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 242, 853
PsrI GAACNNNNNNTAC 2 cut(s) 609, 641
PstNI CAGNNNCTG 1 cut(s) 868
RsaI GTAC 3 cut(s) 7, 390, 739
RsaNI GTAC 3 cut(s) 6, 389, 738
SacI GAGCTC 1 cut(s) 450
SaqAI TTAA 3 cut(s) 476, 630, 809
SatI GCNGC 6 cut(s) 41, 104, 317, 360, 394, 701
Sau3AI GATC 2 cut(s) 93, 219
SchI GAGTC 1 cut(s) 874
ScrFI CCNGG 1 cut(s) 121
SduI GDGCHC 2 cut(s) 450, 662
SfcI CTRYAG 1 cut(s) 507
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 7 cut(s) 59, 155, 335, 686, 704, 767, 875
SsiI CCGC 5 cut(s) 40, 347, 406, 700, 734
SspI AATATT 1 cut(s) 884
SspMI CTAG 2 cut(s) 287, 834
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 1 cut(s) 119
StyI CCWWGG 1 cut(s) 717
TaaI ACNGT 5 cut(s) 418, 569, 608, 681, 742
TaiI ACGT 1 cut(s) 19
TaqI TCGA 4 cut(s) 53, 683, 791, 829
TasI AATT 7 cut(s) 59, 155, 335, 686, 704, 767, 875
TauI GCSGC 2 cut(s) 43, 703
TfiI GAWTC 4 cut(s) 188, 206, 560, 793
Tru1I TTAA 3 cut(s) 476, 630, 809
Tru9I TTAA 3 cut(s) 476, 630, 809
TscAI CASTG 5 cut(s) 271, 423, 574, 611, 626
TseI GCWGC 4 cut(s) 103, 316, 359, 393
TspDTI ATGAA 7 cut(s) 84, 165, 198, 450, 519, 630, 680
TspRI CASTG 5 cut(s) 271, 423, 574, 611, 626
Vha464I CTTAAG 1 cut(s) 629
XapI RAATTY 3 cut(s) 59, 155, 686
XbaI TCTAGA 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 2 cut(s) 287, 834
ZraI GACGTC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.