Rorug05G0015300

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018392
Physical Location & Seq
Forward (+)
1040503 .. 1043657
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0015300.1

Sequence Viewer

Length: 1866 bp
ATGATGATCTCTGTCTCCATCATGTGCTCCCTGTATCCTTCCTACGTCAACCCTGTTACAAAAAGTGAGAAGTACTCCTACGACCCTGAACACATACGCAGTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAGCTTGCACTTTTCATAGCTAGAAAGCCAGAGTATGCAAAAAATCCGAAAATTGTTCAACTAAAAGACGAAAATTCGAAGACGCCTCTTCAGCTCCTCTCATCCAATTCTTCAGCATTCGAGAGTGGAATGAAATGTGGACTGATCAAGAGGTTCATTTACTACTGTGCTCCATATGAGAATGCTAAAGAAGAAGAAGCTCATGTACAAAATTCCAGTGATGATAGTCATGTACAAAGTTCCAGTAGAGCTACTCTGTTTCTAAGGCGAGTCATCTCTTCAGTACATAGATCTGTTTGGATTGTTCTGCGAGAATGGCGAACGATGGAGAAGATTCATGACGAAAGGAAGAGGCATGGACTTGCTTTAGAACTAGCCAAGTTTCTAATTGCAGAAGACTTTTCATGGGACATCATCATGCAACAATCGAATCCAAAAAAAGTAGCGCCATCCCAGTCAGGGACTCCAGACATACAACAATCCAATCCAAAAACAAAAGCGCAATCCCAGTCAGGGAATCCAGACATACAACAACCCAATCCAAAAACAAAAGCGCCATCCCAGTCAGGGACTCCAGACATACAACAATCCAATCCAAAAACAAAAGCGCCAGATATCCCATTGTTCATTGCAACAAGCAATGGAATATTAGAAATTGTGGAGGAGACACTTAGAGTCTACCCTTCGTCACTTGAGTATACAAACAAAGAGAAGCAGAACATACTGCACCTCGCTATAATGCACCGTCAGTCGCACATCTTTGATCGAGTGATGACGAAGAGGAACGACTGGTTAACGTCAAGGTTGACTCGAGAAACCGATAAGAATGGATTCACAATACTGCACTATGTTGGTTTAATGGAATTTTATAAAGGAGGCACCCAGCCTGGTCCTGCACTTCAGTTGCAAGAAGAGTTAGAATGGTATGAGAAGGTGAAGAAGTCAGTCCCACGATATTTTGCAATGAACCGCAGCAGCAGTAATGAACAAAAAGAGACACTCGAAGAGTTCTATGATCGAAAATATAAGGAAACTGTAAAGGAGTTGTCTGAGAATGTGGATAAGATAATTCCACCCAAATCTGTAAATAAACTTGGCTTGGACGAAAGCAAAGAGGAGACCCCCAGAGAGTACTTCGGTCGAACCCATGGGGAGCTCCTCGAGGAAGCACAAGAATGGCTAGAGCGGACCTCCAACTCGTGCTCCTTGGTGGCCGGACTAATTGCCTCCGTCGCCTTCGCGGCCGCTTTCACCATCCCGGGCGGCAACAACGGCAGAAACGGCCAGCCCATCCTCATCGACAGCCCTTTTTTCTTGGTCTTCATCATCACTGACGCTCTCTCCATCGCCAGCTCGCTGACTTCACTCGCCATGTTCCTCTCCATCCTGTCGTCACCGTACGAGCTCGACGACTTCCTCCGCTCTCTCCCTCGGAAGCTCATCCTCGGCTTCACCTCCCTCTTCTTCTCGGTGGCTGTCACCAGCCTCACCTTTGCCTCCACAATGATGCTGACAATTCCGATGAAGAAGCGCCTGACTACAACTCTTATATACTGCGTTGCGGTTCTTCCGGTCACCATGTCTGCGCTGTTGCAGCATCCGTTGTGGGAGGCCTTAAAAAGGACTGGAAAGTCCTCAGTCAAAGTCGTGACAAGAATTCTTCCATCAGTTCCATGGCCACTTATCGAAACTGCTTGCTCAAAGGCCACTAAGGCCCACACTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

621

Amino Acids

70.17

Weight (kDa)

8.79

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 438 - 550 4.1e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018392)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05601
rosa_chinensis RchiOBHm_Chr5g0014071
rosa_laevigata RLG00000032079
rosa_multiflora Rmu_sc0007767.1_g000009
rosa_rugosa Rorug05G0015300 Rorug05G0015400
rosa_samantha Rh5AG109400 Rh5BG106200 Rh5DG104900
rosa_wichuraiana Rw5G009550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1008
AasI GACNNNNNNGTC 1 cut(s) 1768
AbsI CCTCGAGG 1 cut(s) 1298
AccB1I GGYRCC 1 cut(s) 1016
AccBSI CCGCTC 2 cut(s) 1324, 1560
AccI GTMKAC 2 cut(s) 816, 836
AccII CGCG 1 cut(s) 1379
AciI CCGC 7 cut(s) 1108, 1324, 1379, 1383, 1401, 1558, 1700
AcoI YGGCCR 4 cut(s) 1350, 1380, 1420, 1814
AcsI RAATTY 4 cut(s) 211, 349, 1001, 1794
AcuI CTGAAG 4 cut(s) 212, 234, 402, 1022
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 7 cut(s) 74, 103, 345, 372, 423, 1271, 1538
AfiI CCNNNNNNNGG 4 cut(s) 597, 651, 705, 1758
AgsI TTSAA 1 cut(s) 197
AjnI CCWGG 1 cut(s) 1024
AluBI AGCT 9 cut(s) 142, 158, 232, 338, 389, 1294, 1491, 1543, 1576
AluI AGCT 9 cut(s) 142, 158, 232, 338, 389, 1294, 1491, 1543, 1576
Alw21I GWGCWC 5 cut(s) 29, 310, 1296, 1343, 1545
Alw26I GTCTC 4 cut(s) 19, 797, 1127, 1250
Ama87I CYCGRG 3 cut(s) 948, 1298, 1396
AoxI GGCC 7 cut(s) 1350, 1380, 1420, 1749, 1814, 1842, 1851
ApeKI GCWGC 4 cut(s) 116, 1110, 1113, 1732
ApoI RAATTY 4 cut(s) 211, 349, 1001, 1794
Asp700I GAANNNNTTC 1 cut(s) 968
AspLEI GCGC 6 cut(s) 586, 640, 694, 748, 1671, 1726
AspS9I GGNCC 3 cut(s) 1028, 1326, 1852
AsuC2I CCSGG 2 cut(s) 1397, 1398
AsuHPI GGTGA 7 cut(s) 1084, 1381, 1524, 1582, 1609, 1618, 1705
AsuII TTCGAA 1 cut(s) 215
AvaI CYCGRG 3 cut(s) 948, 1298, 1396
AvaII GGWCC 2 cut(s) 1028, 1326
BalI TGGCCA 1 cut(s) 1816
BanI GGYRCC 1 cut(s) 1016
BanII GRGCYC 2 cut(s) 1296, 1545
BarI GAAGNNNNNNTAC 4 cut(s) 62, 94, 327, 359
BauI CACGAG 1 cut(s) 1336
BbsI GAAGAC 3 cut(s) 224, 540, 1450
Bbv12I GWGCWC 5 cut(s) 29, 310, 1296, 1343, 1545
BbvI GCAGC 4 cut(s) 103, 1122, 1125, 1744
BccI CCATC 9 cut(s) 26, 457, 595, 703, 1400, 1436, 1490, 1529, 1810
BceAI ACGGC 2 cut(s) 1426, 1435
BciT130I CCWGG 1 cut(s) 1026
BciVI GTATCC 1 cut(s) 45
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 2 cut(s) 1397, 1398
BcoDI GTCTC 4 cut(s) 19, 797, 1127, 1250
BfaI CTAG 3 cut(s) 159, 512, 1319
BfoI RGCGCY 4 cut(s) 587, 695, 749, 1672
BfuI GTATCC 1 cut(s) 45
BglI GCCNNNNNGGC 2 cut(s) 1379, 1850
BglII AGATCT 1 cut(s) 428
BisI GCNGC 7 cut(s) 117, 1111, 1114, 1380, 1383, 1402, 1733
BlsI GCNGC 7 cut(s) 118, 1112, 1115, 1381, 1384, 1403, 1734
BmcAI AGTACT 2 cut(s) 74, 1271
Bme1390I CCNGG 3 cut(s) 1026, 1397, 1398
Bme18I GGWCC 2 cut(s) 1028, 1326
BmeT110I CYCGRG 3 cut(s) 948, 1298, 1396
BmgT120I GGNCC 3 cut(s) 1028, 1326, 1852
BmiI GGNNCC 1 cut(s) 1018
BmrFI CCNGG 3 cut(s) 1026, 1397, 1398
BmrI ACTGGG 3 cut(s) 586, 640, 694
BmsI GCATC 2 cut(s) 1635, 1744
BmuI ACTGGG 3 cut(s) 586, 640, 694
BpiI GAAGAC 3 cut(s) 224, 540, 1450
BplI GAGNNNNNCTC 4 cut(s) 59, 91, 1313, 1345
BpmI CTGGAG 2 cut(s) 588, 696
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 851
BpuMI CCSGG 2 cut(s) 1397, 1398
BsaHI GRCGYC 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 1250
BsaJI CCNNGG 6 cut(s) 1285, 1344, 1396, 1568, 1582, 1811
BsaWI WCCGGW 1 cut(s) 1708
BsaXI ACNNNNNCTCC 4 cut(s) 1325, 1355, 1463, 1493
Bsc4I CCNNNNNNNGG 4 cut(s) 597, 651, 705, 1758
Bse1I ACTGG 7 cut(s) 354, 381, 592, 646, 700, 932, 1768
Bse3DI GCAATG 3 cut(s) 765, 784, 1107
BseBI CCWGG 1 cut(s) 1026
BseDI CCNNGG 6 cut(s) 1285, 1344, 1396, 1568, 1582, 1811
BseGI GGATG 8 cut(s) 239, 587, 695, 1392, 1428, 1521, 1578, 1735
BseLI CCNNNNNNNGG 4 cut(s) 597, 651, 705, 1758
BseMI GCAATG 3 cut(s) 765, 784, 1107
BseMII CTCAG 2 cut(s) 1179, 1788
BseNI ACTGG 7 cut(s) 354, 381, 592, 646, 700, 932, 1768
BseRI GAGGAG 4 cut(s) 224, 815, 1268, 1286
BseX3I CGGCCG 1 cut(s) 1380
BseXI GCAGC 4 cut(s) 103, 1122, 1125, 1744
BseYI CCCAGC 1 cut(s) 1020
BsgI GTGCAG 3 cut(s) 848, 965, 1017
Bsh1236I CGCG 1 cut(s) 1379
Bsh1285I CGRYCG 2 cut(s) 1279, 1383
BshFI GGCC 7 cut(s) 1352, 1382, 1422, 1751, 1816, 1844, 1853
BshNI GGYRCC 1 cut(s) 1016
BsiEI CGRYCG 2 cut(s) 1279, 1383
BsiHKAI GWGCWC 5 cut(s) 29, 310, 1296, 1343, 1545
BsiHKCI CYCGRG 3 cut(s) 948, 1298, 1396
BsiSI CCGG 3 cut(s) 1353, 1397, 1709
BsiWI CGTACG 1 cut(s) 1536
BslFI GGGAC 4 cut(s) 560, 613, 721, 1070
BslI CCNNNNNNNGG 4 cut(s) 597, 651, 705, 1758
BsmAI GTCTC 4 cut(s) 19, 797, 1127, 1250
BsmFI GGGAC 4 cut(s) 560, 613, 721, 1070
BsmI GAATGC 2 cut(s) 254, 325
BsnI GGCC 7 cut(s) 1352, 1382, 1422, 1751, 1816, 1844, 1853
Bso31I GGTCTC 1 cut(s) 1250
BsoBI CYCGRG 3 cut(s) 948, 1298, 1396
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 5 cut(s) 29, 310, 1296, 1343, 1545
Bsp1407I TGTACA 2 cut(s) 343, 370
Bsp143I GATC 5 cut(s) 6, 282, 428, 901, 1153
Bsp19I CCATGG 2 cut(s) 1285, 1811
BspACI CCGC 7 cut(s) 1108, 1324, 1379, 1383, 1401, 1558, 1700
BspANI GGCC 7 cut(s) 1352, 1382, 1422, 1751, 1816, 1844, 1853
BspCNI CTCAG 2 cut(s) 1180, 1787
BspFNI CGCG 1 cut(s) 1379
BspHI TCATGA 2 cut(s) 475, 1862
BspLI GGNNCC 1 cut(s) 1018
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 1016
BspTNI GGTCTC 1 cut(s) 1250
BsrBI CCGCTC 2 cut(s) 1324, 1560
BsrDI GCAATG 3 cut(s) 765, 784, 1107
BsrGI TGTACA 2 cut(s) 343, 370
BsrI ACTGG 7 cut(s) 354, 381, 592, 646, 700, 932, 1768
BssECI CCNNGG 6 cut(s) 1285, 1344, 1396, 1568, 1582, 1811
BssMI GATC 5 cut(s) 6, 282, 428, 901, 1153
BssNAI GTATAC 1 cut(s) 837
BssNI GRCGYC 1 cut(s) 221
BssSI CACGAG 1 cut(s) 1336
BssT1I CCWWGG 3 cut(s) 1285, 1344, 1811
Bst1107I GTATAC 1 cut(s) 837
Bst2BI CACGAG 1 cut(s) 1336
Bst2UI CCWGG 1 cut(s) 1026
Bst4CI ACNGT 4 cut(s) 305, 884, 1174, 1536
Bst6I CTCTTC 7 cut(s) 231, 421, 482, 911, 1044, 1137, 1604
BstACI GRCGYC 1 cut(s) 221
BstAPI GCANNNNNTGC 1 cut(s) 125
BstAUI TGTACA 2 cut(s) 343, 370
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 5 cut(s) 144, 1424, 1489, 1493, 1834
BstDEI CTNAG 5 cut(s) 401, 809, 1188, 1774, 1848
BstDSI CCRYGG 2 cut(s) 1285, 1811
BstEII GGTNACC 1 cut(s) 1711
BstENI CCTNNNNNAGG 1 cut(s) 1756
BstF5I GGATG 8 cut(s) 239, 587, 695, 1392, 1428, 1521, 1578, 1735
BstFNI CGCG 1 cut(s) 1379
BstH2I RGCGCY 4 cut(s) 587, 695, 749, 1672
BstHHI GCGC 6 cut(s) 586, 640, 694, 748, 1671, 1726
BstKTI GATC 5 cut(s) 9, 285, 431, 904, 1156
BstMAI GTCTC 4 cut(s) 19, 797, 1127, 1250
BstMBI GATC 5 cut(s) 6, 282, 428, 901, 1153
BstMCI CGRYCG 2 cut(s) 1279, 1383
BstMWI GCNNNNNNNGC 9 cut(s) 125, 229, 454, 1370, 1379, 1410, 1419, 1732, 1850
BstNI CCWGG 1 cut(s) 1026
BstNSI RCATGY 1 cut(s) 128
BstPI GGTNACC 1 cut(s) 1711
BstSCI CCNGG 3 cut(s) 1024, 1395, 1396
BstUI CGCG 1 cut(s) 1379
BstV1I GCAGC 4 cut(s) 103, 1122, 1125, 1744
BstV2I GAAGAC 3 cut(s) 224, 540, 1450
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BstZ17I GTATAC 1 cut(s) 837
BstZI CGGCCG 1 cut(s) 1380
BsuI GTATCC 1 cut(s) 45
BsuRI GGCC 7 cut(s) 1352, 1382, 1422, 1751, 1816, 1844, 1853
BtgI CCRYGG 2 cut(s) 1285, 1811
BtgZI GCGATG 1 cut(s) 1468
BtsCI GGATG 8 cut(s) 239, 587, 695, 1392, 1428, 1521, 1578, 1735
BtsIMutI CAGTG 2 cut(s) 361, 1467
Cac8I GCNNGC 5 cut(s) 144, 1424, 1489, 1493, 1834
CciI TCATGA 2 cut(s) 475, 1862
CciNI GCGGCCGC 1 cut(s) 1380
CfoI GCGC 6 cut(s) 586, 640, 694, 748, 1671, 1726
Cfr13I GGNCC 3 cut(s) 1028, 1326, 1852
Cfr9I CCCGGG 1 cut(s) 1396
CseI GACGC 2 cut(s) 229, 1481
Csp6I GTAC 7 cut(s) 73, 102, 344, 371, 422, 1270, 1537
CviQI GTAC 7 cut(s) 73, 102, 344, 371, 422, 1270, 1537
DdeI CTNAG 5 cut(s) 401, 809, 1188, 1774, 1848
DpnI GATC 5 cut(s) 8, 284, 430, 903, 1155
DpnII GATC 5 cut(s) 6, 282, 428, 901, 1153
DrdI GACNNNNNNGTC 1 cut(s) 1768
DseDI GACNNNNNNGTC 1 cut(s) 1768
EaeI YGGCCR 4 cut(s) 1350, 1380, 1420, 1814
EagI CGGCCG 1 cut(s) 1380
Eam1104I CTCTTC 7 cut(s) 231, 421, 482, 911, 1044, 1137, 1604
EarI CTCTTC 7 cut(s) 231, 421, 482, 911, 1044, 1137, 1604
Ecl136II GAGCTC 2 cut(s) 1294, 1543
EclXI CGGCCG 1 cut(s) 1380
Eco130I CCWWGG 3 cut(s) 1285, 1344, 1811
Eco147I AGGCCT 1 cut(s) 1751
Eco24I GRGCYC 2 cut(s) 1296, 1545
Eco31I GGTCTC 1 cut(s) 1250
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 2 cut(s) 1028, 1326
Eco52I CGGCCG 1 cut(s) 1380
Eco53kI GAGCTC 2 cut(s) 1294, 1543
Eco57I CTGAAG 4 cut(s) 212, 234, 402, 1022
Eco88I CYCGRG 3 cut(s) 948, 1298, 1396
Eco91I GGTNACC 1 cut(s) 1711
EcoICRI GAGCTC 2 cut(s) 1294, 1543
EcoNI CCTNNNNNAGG 1 cut(s) 1756
EcoO65I GGTNACC 1 cut(s) 1711
EcoRI GAATTC 1 cut(s) 1794
EcoRII CCWGG 1 cut(s) 1024
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 3 cut(s) 1285, 1344, 1811
EcoT38I GRGCYC 2 cut(s) 1296, 1545
ErhI CCWWGG 3 cut(s) 1285, 1344, 1811
FaqI GGGAC 4 cut(s) 560, 613, 721, 1070
FauNDI CATATG 1 cut(s) 313
FbaI TGATCA 1 cut(s) 282
FblI GTMKAC 2 cut(s) 816, 836
Fnu4HI GCNGC 7 cut(s) 117, 1111, 1114, 1380, 1383, 1402, 1733
FokI GGATG 8 cut(s) 226, 574, 682, 1379, 1415, 1508, 1565, 1722
FriOI GRGCYC 2 cut(s) 1296, 1545
Fsp4HI GCNGC 7 cut(s) 117, 1111, 1114, 1380, 1383, 1402, 1733
FspBI CTAG 3 cut(s) 159, 512, 1319
GlaI GCGC 6 cut(s) 585, 639, 693, 747, 1670, 1725
GluI GCNGC 7 cut(s) 117, 1111, 1114, 1380, 1383, 1402, 1733
GsaI CCCAGC 1 cut(s) 1024
GsuI CTGGAG 2 cut(s) 588, 696
HaeII RGCGCY 4 cut(s) 587, 695, 749, 1672
HaeIII GGCC 7 cut(s) 1352, 1382, 1422, 1751, 1816, 1844, 1853
HapII CCGG 3 cut(s) 1353, 1397, 1709
HgaI GACGC 2 cut(s) 229, 1481
HhaI GCGC 6 cut(s) 586, 640, 694, 748, 1671, 1726
Hin1I GRCGYC 1 cut(s) 221
Hin6I GCGC 6 cut(s) 584, 638, 692, 746, 1669, 1724
HinP1I GCGC 6 cut(s) 584, 638, 692, 746, 1669, 1724
HincII GTYRAC 3 cut(s) 49, 933, 945
HindII GTYRAC 3 cut(s) 49, 933, 945
HinfI GANTC 9 cut(s) 408, 472, 568, 601, 655, 709, 813, 946, 969
HpaI GTTAAC 1 cut(s) 933
HpaII CCGG 3 cut(s) 1353, 1397, 1709
HphI GGTGA 7 cut(s) 1084, 1381, 1524, 1582, 1609, 1618, 1705
Hpy166II GTNNAC 6 cut(s) 49, 278, 817, 837, 933, 945
Hpy188I TCNGA 4 cut(s) 186, 1189, 1572, 1659
Hpy188III TCNNGA 9 cut(s) 259, 286, 476, 605, 659, 713, 950, 1786, 1863
Hpy8I GTNNAC 6 cut(s) 49, 278, 817, 837, 933, 945
Hpy99I CGWCG 2 cut(s) 1373, 1550
HpyAV CCTTC 4 cut(s) 48, 831, 1063, 1384
HpyCH4III ACNGT 4 cut(s) 305, 884, 1174, 1536
HpyCH4IV ACGT 2 cut(s) 45, 935
HpyF10VI GCNNNNNNNGC 9 cut(s) 125, 229, 454, 1370, 1379, 1410, 1419, 1732, 1850
HpyF3I CTNAG 5 cut(s) 401, 809, 1188, 1774, 1848
HpySE526I ACGT 2 cut(s) 45, 935
Hsp92I GRCGYC 1 cut(s) 221
HspAI GCGC 6 cut(s) 584, 638, 692, 746, 1669, 1724
Ksp22I TGATCA 1 cut(s) 282
KspAI GTTAAC 1 cut(s) 933
Kzo9I GATC 5 cut(s) 6, 282, 428, 901, 1153
LmnI GCTCC 6 cut(s) 32, 237, 313, 1291, 1299, 1346
Lsp1109I GCAGC 4 cut(s) 103, 1122, 1125, 1744
LweI GCATC 2 cut(s) 1635, 1744
MaeI CTAG 3 cut(s) 159, 512, 1319
MaeII ACGT 2 cut(s) 45, 935
MaeIII GTNAC 6 cut(s) 55, 825, 1530, 1615, 1711, 1786
MalI GATC 5 cut(s) 8, 284, 430, 903, 1155
MbiI CCGCTC 2 cut(s) 1324, 1560
MboI GATC 5 cut(s) 6, 282, 428, 901, 1153
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 5 cut(s) 29, 310, 1296, 1343, 1545
MlsI TGGCCA 1 cut(s) 1816
MluNI TGGCCA 1 cut(s) 1816
MlyI GAGTC 5 cut(s) 417, 595, 703, 822, 940
MmeI TCCRAC 1 cut(s) 1356
Mox20I TGGCCA 1 cut(s) 1816
MroXI GAANNNNTTC 1 cut(s) 968
MscI TGGCCA 1 cut(s) 1816
MseI TTAA 3 cut(s) 932, 995, 1754
MslI CAYNNNNRTG 3 cut(s) 554, 1312, 1643
Msp20I TGGCCA 1 cut(s) 1816
MspI CCGG 3 cut(s) 1353, 1397, 1709
MspR9I CCNGG 3 cut(s) 1026, 1397, 1398
Mva1269I GAATGC 2 cut(s) 254, 325
MvaI CCWGG 1 cut(s) 1026
MvnI CGCG 1 cut(s) 1379
MwoI GCNNNNNNNGC 9 cut(s) 125, 229, 454, 1370, 1379, 1410, 1419, 1732, 1850
NciI CCSGG 2 cut(s) 1397, 1398
NcoI CCATGG 2 cut(s) 1285, 1811
NdeI CATATG 1 cut(s) 313
NdeII GATC 5 cut(s) 6, 282, 428, 901, 1153
NlaIV GGNNCC 1 cut(s) 1018
NmeAIII GCCGAG 2 cut(s) 154, 1563
NmuCI GTSAC 5 cut(s) 825, 1530, 1615, 1711, 1786
NotI GCGGCCGC 1 cut(s) 1380
NspI RCATGY 1 cut(s) 128
NspV TTCGAA 1 cut(s) 215
PaeR7I CTCGAG 2 cut(s) 948, 1298
PagI TCATGA 2 cut(s) 475, 1862
PceI AGGCCT 1 cut(s) 1751
PcsI WCGNNNNNNNCGW 1 cut(s) 1545
PctI GAATGC 2 cut(s) 254, 325
PdmI GAANNNNTTC 1 cut(s) 968
PfeI GAWTC 4 cut(s) 472, 568, 655, 969
Pfl23II CGTACG 1 cut(s) 1536
PkrI GCNGC 7 cut(s) 118, 1112, 1115, 1381, 1384, 1403, 1734
PleI GAGTC 5 cut(s) 416, 595, 703, 821, 940
PpsI GAGTC 5 cut(s) 416, 595, 703, 821, 940
PsiI TTATAA 1 cut(s) 1008
Psp124BI GAGCTC 2 cut(s) 1296, 1545
Psp6I CCWGG 1 cut(s) 1024
PspEI GGTNACC 1 cut(s) 1711
PspFI CCCAGC 1 cut(s) 1020
PspGI CCWGG 1 cut(s) 1024
PspLI CGTACG 1 cut(s) 1536
PspN4I GGNNCC 1 cut(s) 1018
PspPI GGNCC 3 cut(s) 1028, 1326, 1852
PspXI VCTCGAGB 1 cut(s) 1298
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 7 cut(s) 74, 103, 345, 372, 423, 1271, 1538
RsaNI GTAC 7 cut(s) 73, 102, 344, 371, 422, 1270, 1537
RseI CAYNNNNRTG 3 cut(s) 554, 1312, 1643
SacI GAGCTC 2 cut(s) 1296, 1545
SaqAI TTAA 3 cut(s) 932, 995, 1754
SatI GCNGC 7 cut(s) 117, 1111, 1114, 1380, 1383, 1402, 1733
Sau3AI GATC 5 cut(s) 6, 282, 428, 901, 1153
Sau96I GGNCC 3 cut(s) 1028, 1326, 1852
ScaI AGTACT 2 cut(s) 74, 1271
SchI GAGTC 5 cut(s) 417, 595, 703, 822, 940
ScrFI CCNGG 3 cut(s) 1026, 1397, 1398
SduI GDGCHC 5 cut(s) 29, 310, 1296, 1343, 1545
SfaNI GCATC 2 cut(s) 1635, 1744
SfiI GGCCNNNNNGGCC 1 cut(s) 1850
Sfr274I CTCGAG 2 cut(s) 948, 1298
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 2 cut(s) 1028, 1326
SlaI CTCGAG 2 cut(s) 948, 1298
SmaI CCCGGG 1 cut(s) 1398
SmiMI CAYNNNNRTG 3 cut(s) 554, 1312, 1643
SmlI CTYRAG 3 cut(s) 830, 948, 1298
SmoI CTYRAG 3 cut(s) 830, 948, 1298
SseBI AGGCCT 1 cut(s) 1751
SsiI CCGC 7 cut(s) 1108, 1324, 1379, 1383, 1401, 1558, 1700
SspI AATATT 1 cut(s) 786
SspMI CTAG 3 cut(s) 159, 512, 1319
SstI GAGCTC 2 cut(s) 1296, 1545
StuI AGGCCT 1 cut(s) 1751
StyD4I CCNGG 3 cut(s) 1024, 1395, 1396
StyI CCWWGG 3 cut(s) 1285, 1344, 1811
TaaI ACNGT 4 cut(s) 305, 884, 1174, 1536
TaiI ACGT 2 cut(s) 48, 938
TaqII GACCGA 1 cut(s) 1265
TatI WGTACW 5 cut(s) 72, 343, 370, 421, 1269
TauI GCSGC 3 cut(s) 1382, 1385, 1404
TfiI GAWTC 4 cut(s) 472, 568, 655, 969
Tru1I TTAA 3 cut(s) 932, 995, 1754
Tru9I TTAA 3 cut(s) 932, 995, 1754
TscAI CASTG 2 cut(s) 361, 1474
TseFI GTSAC 5 cut(s) 825, 1530, 1615, 1711, 1786
TseI GCWGC 4 cut(s) 116, 1110, 1113, 1732
Tsp45I GTSAC 5 cut(s) 825, 1530, 1615, 1711, 1786
TspGWI ACGGA 2 cut(s) 1357, 1728
TspMI CCCGGG 1 cut(s) 1396
TspRI CASTG 2 cut(s) 361, 1474
VpaK11BI GGWCC 2 cut(s) 1028, 1326
XagI CCTNNNNNAGG 1 cut(s) 1756
XapI RAATTY 4 cut(s) 211, 349, 1001, 1794
XceI RCATGY 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 1 cut(s) 1809
XhoI CTCGAG 2 cut(s) 948, 1298
XmaI CCCGGG 1 cut(s) 1396
XmiI GTMKAC 2 cut(s) 816, 836
XmnI GAANNNNTTC 1 cut(s) 968
XspI CTAG 3 cut(s) 159, 512, 1319
ZrmI AGTACT 2 cut(s) 74, 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.