Rh5BG106200

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018392
Physical Location & Seq
Forward (+)
9735178 .. 9737750
2573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG106200.1

Sequence Viewer

Length: 1047 bp
ATGGTTCTGATCAGATTGCAGCTCTACAAGCTTAACGGGAGTGGTTGGATGGATGGAGACGCCTATGATGACGTTCCAGTCGCAAGAGATGTTACTTATGAAGAATCTCCCATCTGGATAGAGTCTCACTTTTGGATCAGCCTCTCATCTCTCAAGCACTTGCCGAAAAGGAAGAAGCACACTGAGCTTTTTACAGAGGTTCTAGAGGATTGGAGTGTGCCAAAACCTCAGCAGCCAGCTATTATTGCACAATTGTTTCAGGTGGTTGATGGAGCTGCCCCCAAGTTGTCTATCGTTGTCGCCGTGCGCAGCCTCACTCTGCAGTTGCGCATTGTGAGTAGCTTTGGTCATGAAGCGACGGAGCTCAATCCTACAGCTGAATCATCCACATTCGTTGAGGAGGGTTTGGAGAAAGTGGGCCTTGATAACTCGGGAGCTACAGAACACAAGAAAACCTCTTCATGTGTTTTGTGCATGGAGGGCTTTTTCGAATCTGACAACGGTGCTAAAAAACTTACTCGCTTGCCCTGCTCGCACTCTTATCATGGACGCTGCTTACTTCAGTGGCCATCAGAGATAAGTAGCTCGTGTCCCTTGTGCGAAAAGCCCAAGTCGAAATTTCATGCTTGTCGGCAATTTTGGGTTGGGATGGTGAGGAAGCACGCGGGTACTGTGATATCTGCACTGATTTTTTGCCAATTTTTCAAGCCTACTCTTCAAGTCCAGCCTTTAGAGACCACCCCTGCCCGTCCTAAAAGCTGGAAGCAGAGTCTGCTTGAAATGGATGAAATGATGACCATGACGGCAACCAAATTGGTAAACGATTTCCAATCCGGCAAACTATTGAAGCAAATTAAGAAAGAAGAGTGGTGGCAATTCAAGAAGGAATGCGAAACCAAATGTAGCCAGACATTAGAGAAAAAAGAAGTGCGCGGTTCAGAAGCTACCATCTCTAAGGATAGAAACTATGCACAGATGGCCAATACCCCAATGAAAGGAAAGAATCGACATATGTGTCTCTTCAAACCTTTTTCGTTCGTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

348

Amino Acids

39.64

Weight (kDa)

8.85

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 154 - 200 2.5e-09 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018392)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05601
rosa_chinensis RchiOBHm_Chr5g0014071
rosa_laevigata RLG00000032079
rosa_multiflora Rmu_sc0007767.1_g000009
rosa_rugosa Rorug05G0015300 Rorug05G0015400
rosa_samantha Rh5AG109400 Rh5BG106200 Rh5DG104900
rosa_wichuraiana Rw5G009550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 77, 1014
Acc16I TGCGCA 2 cut(s) 308, 329
AccII CGCG 2 cut(s) 665, 933
AciI CCGC 2 cut(s) 665, 933
AclWI GGATC 1 cut(s) 143
AcoI YGGCCR 2 cut(s) 566, 978
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 1 cut(s) 545
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 670
AfiI CCNNNNNNNGG 1 cut(s) 995
AgsI TTSAA 6 cut(s) 706, 719, 779, 847, 880, 1024
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 4 cut(s) 51, 129, 728, 1022
AlwI GGATC 1 cut(s) 143
AlwNI CAGNNNCTG 1 cut(s) 772
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 3 cut(s) 418, 566, 978
ApeKI GCWGC 5 cut(s) 19, 232, 275, 309, 552
ApoI RAATTY 1 cut(s) 617
AspLEI GCGC 3 cut(s) 309, 330, 933
AspS9I GGNCC 1 cut(s) 418
AsuHPI GGTGA 1 cut(s) 664
AsuII TTCGAA 1 cut(s) 489
AvaI CYCGRG 1 cut(s) 430
BalI TGGCCA 2 cut(s) 568, 980
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 586
Bbv12I GWGCWC 1 cut(s) 366
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 5 cut(s) 31, 244, 262, 321, 539
BccI CCATC 8 cut(s) 43, 47, 119, 263, 577, 643, 956, 970
BceAI ACGGC 2 cut(s) 287, 819
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 4 cut(s) 51, 129, 728, 1022
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 3 cut(s) 320, 372, 438
BisI GCNGC 5 cut(s) 20, 233, 276, 310, 553
BlsI GCNGC 5 cut(s) 21, 234, 277, 311, 554
BmeT110I CYCGRG 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 228
Bpu14I TTCGAA 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 728
Bsc4I CCNNNNNNNGG 1 cut(s) 995
Bse1I ACTGG 1 cut(s) 77
BseGI GGATG 5 cut(s) 54, 58, 383, 654, 790
BseLI CCNNNNNNNGG 1 cut(s) 995
BseMII CTCAG 2 cut(s) 174, 242
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 5 cut(s) 31, 244, 262, 321, 539
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 2 cut(s) 665, 933
BshFI GGCC 3 cut(s) 420, 568, 980
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 430
BsiSI CCGG 1 cut(s) 834
BslFI GGGAC 1 cut(s) 576
BslI CCNNNNNNNGG 1 cut(s) 995
BsmAI GTCTC 4 cut(s) 51, 129, 728, 1022
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 1 cut(s) 893
BsnI GGCC 3 cut(s) 420, 568, 980
Bso31I GGTCTC 1 cut(s) 728
BsoBI CYCGRG 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 489
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 2 cut(s) 9, 135
BspACI CCGC 2 cut(s) 665, 933
BspANI GGCC 3 cut(s) 420, 568, 980
BspCNI CTCAG 2 cut(s) 175, 241
BspFNI CGCG 2 cut(s) 665, 933
BspHI TCATGA 1 cut(s) 349
BspMAI CTGCAG 1 cut(s) 324
BspPI GGATC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 489
BspTNI GGTCTC 1 cut(s) 728
BsrI ACTGG 1 cut(s) 77
BssMI GATC 2 cut(s) 9, 135
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 586
Bst2BI CACGAG 1 cut(s) 586
Bst4CI ACNGT 2 cut(s) 503, 673
Bst6I CTCTTC 4 cut(s) 463, 720, 858, 1025
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 772
BstBI TTCGAA 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 237, 524, 533, 663
BstDEI CTNAG 3 cut(s) 183, 228, 954
BstF5I GGATG 5 cut(s) 54, 58, 383, 654, 790
BstFNI CGCG 2 cut(s) 665, 933
BstHHI GCGC 3 cut(s) 309, 330, 933
BstKTI GATC 2 cut(s) 12, 138
BstMAI GTCTC 4 cut(s) 51, 129, 728, 1022
BstMBI GATC 2 cut(s) 9, 135
BstMWI GCNNNNNNNGC 8 cut(s) 28, 184, 245, 480, 528, 532, 772, 977
BstSFI CTRYAG 3 cut(s) 320, 372, 438
BstUI CGCG 2 cut(s) 665, 933
BstV1I GCAGC 5 cut(s) 31, 244, 262, 321, 539
BsuRI GGCC 3 cut(s) 420, 568, 980
BtsCI GGATG 5 cut(s) 54, 58, 383, 654, 790
BtsIMutI CAGTG 3 cut(s) 180, 569, 683
Cac8I GCNNGC 4 cut(s) 237, 524, 533, 663
CaiI CAGNNNCTG 1 cut(s) 772
CciI TCATGA 1 cut(s) 349
CfoI GCGC 3 cut(s) 309, 330, 933
Cfr13I GGNCC 1 cut(s) 418
CseI GACGC 2 cut(s) 68, 558
Csp6I GTAC 1 cut(s) 669
CspCI CAANNNNNGTGG 2 cut(s) 376, 411
CviAII CATG 6 cut(s) 350, 462, 475, 545, 623, 799
CviQI GTAC 1 cut(s) 669
DdeI CTNAG 3 cut(s) 183, 228, 954
DpnI GATC 2 cut(s) 11, 137
DpnII GATC 2 cut(s) 9, 135
DrdI GACNNNNNNGTC 2 cut(s) 77, 1014
DseDI GACNNNNNNGTC 2 cut(s) 77, 1014
EaeI YGGCCR 2 cut(s) 566, 978
Eam1104I CTCTTC 4 cut(s) 463, 720, 858, 1025
EarI CTCTTC 4 cut(s) 463, 720, 858, 1025
Ecl136II GAGCTC 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 728
Eco32I GATATC 1 cut(s) 678
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 545
Eco88I CYCGRG 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 364
EcoRV GATATC 1 cut(s) 678
EcoT38I GRGCYC 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 6 cut(s) 353, 465, 478, 548, 626, 802
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 1 cut(s) 576
FatI CATG 6 cut(s) 349, 461, 474, 544, 622, 798
FauI CCCGC 1 cut(s) 658
FauNDI CATATG 1 cut(s) 1011
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 5 cut(s) 20, 233, 276, 310, 553
FokI GGATG 5 cut(s) 61, 65, 370, 661, 797
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 5 cut(s) 20, 233, 276, 310, 553
FspBI CTAG 1 cut(s) 203
FspI TGCGCA 2 cut(s) 308, 329
GlaI GCGC 3 cut(s) 308, 329, 932
GluI GCNGC 5 cut(s) 20, 233, 276, 310, 553
HaeIII GGCC 3 cut(s) 420, 568, 980
HapII CCGG 1 cut(s) 834
HgaI GACGC 2 cut(s) 68, 558
HhaI GCGC 3 cut(s) 309, 330, 933
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 6 cut(s) 353, 465, 478, 548, 626, 802
Hin6I GCGC 3 cut(s) 307, 328, 931
HinP1I GCGC 3 cut(s) 307, 328, 931
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 6 cut(s) 104, 122, 380, 491, 769, 1003
HpaII CCGG 1 cut(s) 834
HphI GGTGA 1 cut(s) 664
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 5 cut(s) 9, 14, 496, 574, 940
Hpy188III TCNNGA 6 cut(s) 115, 203, 350, 432, 880, 1039
Hpy8I GTNNAC 1 cut(s) 820
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 1 cut(s) 877
HpyCH4III ACNGT 2 cut(s) 503, 673
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 6 cut(s) 19, 248, 322, 474, 683, 971
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 184, 245, 480, 528, 532, 772, 977
HpyF3I CTNAG 3 cut(s) 183, 228, 954
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 6 cut(s) 353, 465, 478, 548, 626, 802
HspAI GCGC 3 cut(s) 307, 328, 931
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 2 cut(s) 9, 135
LmnI GCTCC 3 cut(s) 272, 361, 434
Lsp1109I GCAGC 5 cut(s) 31, 244, 262, 321, 539
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 91
MalI GATC 2 cut(s) 11, 137
MboI GATC 2 cut(s) 9, 135
MboII GAAGA 6 cut(s) 113, 184, 450, 707, 875, 1012
MfeI CAATTG 1 cut(s) 251
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 2 cut(s) 568, 980
MluCI AATT 7 cut(s) 251, 617, 635, 698, 812, 852, 875
MluNI TGGCCA 2 cut(s) 568, 980
MlyI GAGTC 2 cut(s) 131, 778
MmeI TCCRAC 1 cut(s) 26
Mox20I TGGCCA 2 cut(s) 568, 980
MscI TGGCCA 2 cut(s) 568, 980
MseI TTAA 2 cut(s) 33, 855
Msp20I TGGCCA 2 cut(s) 568, 980
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 1 cut(s) 834
MunI CAATTG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 893
MvnI CGCG 2 cut(s) 665, 933
MwoI GCNNNNNNNGC 8 cut(s) 28, 184, 245, 480, 528, 532, 772, 977
NdeI CATATG 1 cut(s) 1011
NdeII GATC 2 cut(s) 9, 135
NlaIII CATG 6 cut(s) 353, 465, 478, 548, 626, 802
NsbI TGCGCA 2 cut(s) 308, 329
NspV TTCGAA 1 cut(s) 489
PagI TCATGA 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 893
PfeI GAWTC 4 cut(s) 104, 380, 491, 1003
PkrI GCNGC 5 cut(s) 21, 234, 277, 311, 554
PleI GAGTC 2 cut(s) 130, 777
PpsI GAGTC 2 cut(s) 130, 777
Psp124BI GAGCTC 1 cut(s) 366
PspPI GGNCC 1 cut(s) 418
PstI CTGCAG 1 cut(s) 324
PstNI CAGNNNCTG 1 cut(s) 772
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 670
RsaNI GTAC 1 cut(s) 669
SacI GAGCTC 1 cut(s) 366
SaqAI TTAA 2 cut(s) 33, 855
SatI GCNGC 5 cut(s) 20, 233, 276, 310, 553
Sau3AI GATC 2 cut(s) 9, 135
Sau96I GGNCC 1 cut(s) 418
SchI GAGTC 2 cut(s) 131, 778
SduI GDGCHC 1 cut(s) 366
SfcI CTRYAG 3 cut(s) 320, 372, 438
SfuI TTCGAA 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 7 cut(s) 251, 617, 635, 698, 812, 852, 875
SsiI CCGC 2 cut(s) 665, 933
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 366
TaaI ACNGT 2 cut(s) 503, 673
TaiI ACGT 1 cut(s) 75
TaqI TCGA 3 cut(s) 489, 614, 1006
TasI AATT 7 cut(s) 251, 617, 635, 698, 812, 852, 875
TfiI GAWTC 4 cut(s) 104, 380, 491, 1003
Tru1I TTAA 2 cut(s) 33, 855
Tru9I TTAA 2 cut(s) 33, 855
TscAI CASTG 3 cut(s) 187, 569, 690
TseI GCWGC 5 cut(s) 19, 232, 275, 309, 552
TspDTI ATGAA 6 cut(s) 114, 366, 450, 611, 801, 1007
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 3 cut(s) 187, 569, 690
XapI RAATTY 1 cut(s) 617
XbaI TCTAGA 1 cut(s) 202
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.