FvH4_3g06300

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006562
Physical Location & Seq
Reverse (-)
3618859 .. 3625426
6568 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06300.t1

Sequence Viewer

Length: 2442 bp
ATGAGCGGGAGAGGCGGCGGCGGAGGGAATGGGAAGGGGAGTAGCGGAATATCCGGAATACCAGGTGGGTCCCGGAAGATCGTTCAGAGCTTGAAAGAGATAGTGAATAACTGCACTGAGCAGGAGATCTATGCTATGCTTAAGGAATGTAACATGGACCCTAATGAAGCCGTCAATCGTCTTCTTGCTCAAGATCCTTTTCATGAAGTGAAGAGCAAACGCGAGAAGAAAAAGGAGAGTAAGGAACCAACGGATTCTAGGTCCCGCGGTGCTAATTTTTCAAATTATGGTGGTAGAGGTGGTGATCGCTATGCTTCACGTGGCGGATCAAACCACTTTGGCACTAATGAGTCTGGTTCTTTGCATGGTAGACCTGCGTACAGGAAGGAAAATGGAACACCTGCCTATGCAGGTTCTTCCTCTACTGCGGCTGGCACAACTGGATATAACTCGAGCCGACGACCTCCTACATACAGTGATTCGGTGGCAGCTGAAAATAAAGTCACATCACTAGCTGATGATGGAATATCTTCATCCTCACAGCCTTCCTCTGGATATCAGTCTGCATGGGGAGGGGTGCCAGGGGCACCTCCTGCAGCAGGATACAATCTGAATTCATCGCAGACTAAGCCCTTGGCTGATGAGTGGCCCTTGATTGAGCATCCTCCAGGTGTTAGCATGTCCTCCATTTTAGGGAAACCTGCAAATTCTGAGCTGTATGCTAATTCATCTAGCTTTCCCGTGGATAACACTAATCAGCATGTAACATCCCAACTAGATGAGGTTGATACGGATGATACGGAAGATGATGGTTCCGTTGAGGCACTGAACACAAGCCATGATGGGCCTACTTCTGTTTCCGGTAGAAATGTGCAAGAAGATAACTCGGCCAATGCATCAGCGTTTGATAATAGCTCGTATGAGGATATCGATCCCTACCATACACAGAGACATGTCCTCGAGGATAATGAAGGTTCAGGTGAGCATGATGCTTCATCAGTGGCTGCAAACTTGCAGCAGCTTGACTTACAGAAGGATGATAGGGGAGCACCACCTGAAGACGAGAACCCTCCCCCAGTAGTCATTCCAAATCACCTACAGCTCCATACTCCAGATTGCTTGCACTTGAGCTTTGGAAGTTTCCGATCTGGCCCTGAACCTGATCTTGCAAGTTCTCATCCACTGAAAAGTGACTTGGAAGAAACATCTCCGACAGTAGATGTTTCTGCTGTTGGGCACTCAGATGCTAGAAATCCTGACTACTATGGAGACGAGCATCTCATAAATACCTCTGATGGAAGTTTGGTTCATAGAACTGGGGCCAATGCTGGAGATTATGATTCTCCTTCAGTGCCACAACCAGAGGTCTTAAAACAGGAAACCCCTGAAGCTGATCAGGGCATTCAATATACTTTTCCTTCTGCACCTGGATTTGCCTATGACAGCTCTCAACAACTGAATATGGCATTTTCTCACCAACAGAGTTCACAGATGCAGAACAATGCTCCCTTTTCAAGCATGATGCAGGCATACACAAACTCATTGCCCAGCACACTGTTGGGTTCAAATGCTCAGAATGTAAGGGAGGATCTTCCATACTCACCTTTCCCTGTGACACAATCTATGCCCACAAAATATAGCAACGTGCCTTCTTCCATCAGCGGTCCCACCATGTCCATGCCAGAGGCTTTGAGAGCAGGTGGCATCTCTTCTGCTCAACCTACTCAACAGACCCTACCCGGTGCTGGTGTTGCAACTGGACCTGCTCTTCCACAACACCTGATGCACCCTTACTCTCAACCTAGTCTACCTTTGGGACATTTTTCCAACATGATTGGTTATCCTTTCTTGCCTCAGAGCTACACATATATGCCATCAGCATTCCAGCAAGCTTTTGCTGGTAACAGCACATACCATCAGTCACTGGCTGCAGTTCTCCCACAGTATAAAAATAGTGTTTCTGTCAGCAGCTTGCCCCAGTCTGCAAATGTTCCTTCTGGTTATGGGTTTGGTAGTTCAACTAGCATTCCTGGAGGAAACTTTCCGTTGAATCCTCCATCTGCACCTACCGGCACAACAATCGGTTATGATGATGTTATAAGCTCCCAGTACAAGGACAGTAACCATTTGATGTCACTTCAGCAGCAGAATGACAACTCAGGCATGTGGGTACACGGACCTGGTTCCCGAGCAATGCCCGCTGTTCCAGCCAGCAATTATTACAGTTTCCAGGGGCAGAATCAACAGCATGCTGGTTTCCGCCAAACCCAGCAACCATCGCAGCAATTTGCTGGAGCAGCTCTAAACTACCCAAACTTCTATCATTCTCAGACAGGGATGTCCCTCGATCATCAACAGCAAAGTTCAAGGGACGCATCCCTGGGTGGCTCACAGGGCCAACCTTCTAAGCAGTCTCAACAATTATGGCAAAACAGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

814

Amino Acids

86.57

Weight (kDa)

5.06

Isoelectric Point (pI)

59.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 20 - 79 1.9e-33 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2099
AarI CACCTGC 2 cut(s) 409, 1688
AasI GACNNNNNNGTC 1 cut(s) 2338
AbsI CCTCGAGG 1 cut(s) 959
Acc36I ACCTGC 6 cut(s) 382, 401, 409, 709, 1688, 1771
AccB1I GGYRCC 2 cut(s) 577, 586
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 2 cut(s) 370, 1807
AccII CGCG 2 cut(s) 222, 267
AccIII TCCGGA 1 cut(s) 53
AclWI GGATC 4 cut(s) 188, 334, 926, 1596
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 2 cut(s) 613, 706
AcuI CTGAAG 4 cut(s) 1077, 1332, 1407, 2123
AcvI CACGTG 1 cut(s) 320
AfaI GTAC 3 cut(s) 380, 2111, 2172
AfiI CCNNNNNNNGG 5 cut(s) 551, 599, 693, 1745, 2113
AflII CTTAAG 1 cut(s) 140
AflIII ACRYGT 1 cut(s) 952
AgsI TTSAA 8 cut(s) 94, 282, 1406, 1515, 1566, 2019, 2050, 2367
AjnI CCWGG 8 cut(s) 61, 580, 667, 1426, 2029, 2179, 2229, 2379
AjuI GAANNNNNNNTTGG 2 cut(s) 1178, 1210
AloI GAACNNNNNNTCC 2 cut(s) 1290, 1322
Alw21I GWGCWC 1 cut(s) 1051
Alw26I GTCTC 3 cut(s) 943, 1263, 2418
AlwI GGATC 4 cut(s) 188, 334, 926, 1596
AlwNI CAGNNNCTG 2 cut(s) 1004, 1924
Ama87I CYCGRG 3 cut(s) 451, 959, 2187
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 6 cut(s) 647, 845, 888, 1150, 1320, 2395
ApoI RAATTY 2 cut(s) 613, 706
Asp700I GAANNNNTTC 1 cut(s) 529
AsuC2I CCSGG 2 cut(s) 73, 1740
AsuHPI GGTGA 5 cut(s) 314, 992, 1085, 1466, 1593
AvaI CYCGRG 3 cut(s) 451, 959, 2187
AvaII GGWCC 6 cut(s) 69, 157, 261, 1664, 1760, 2177
BaeGI GKGCMC 2 cut(s) 589, 1239
BanI GGYRCC 2 cut(s) 577, 586
BbrPI CACGTG 1 cut(s) 320
BbsI GAAGAC 2 cut(s) 173, 1065
Bbv12I GWGCWC 1 cut(s) 1051
BccI CCATC 9 cut(s) 515, 803, 836, 1289, 1664, 1882, 1923, 2065, 2284
BceAI ACGGC 1 cut(s) 155
BcgI CGANNNNNNTGC 2 cut(s) 2062, 2096
BciT130I CCWGG 8 cut(s) 63, 582, 669, 1428, 2031, 2181, 2231, 2381
BciVI GTATCC 1 cut(s) 596
BclI TGATCA 1 cut(s) 1393
BcnI CCSGG 2 cut(s) 73, 1740
BcoDI GTCTC 3 cut(s) 943, 1263, 2418
BfaI CTAG 7 cut(s) 258, 512, 732, 776, 1248, 1803, 2022
BfmI CTRYAG 3 cut(s) 594, 1097, 1929
BfrI CTTAAG 1 cut(s) 140
BfuAI ACCTGC 6 cut(s) 382, 401, 409, 709, 1688, 1771
BfuI GTATCC 1 cut(s) 596
BglII AGATCT 1 cut(s) 126
Bme18I GGWCC 6 cut(s) 69, 157, 261, 1664, 1760, 2177
BmeT110I CYCGRG 3 cut(s) 451, 959, 2187
BmrI ACTGGG 4 cut(s) 1070, 1326, 1972, 2101
BmuI ACTGGG 4 cut(s) 1070, 1326, 1972, 2101
BpiI GAAGAC 2 cut(s) 173, 1065
BpmI CTGGAG 5 cut(s) 651, 1095, 1350, 2052, 2313
BpuEI CTTGAG 2 cut(s) 174, 1147
BpuMI CCSGG 2 cut(s) 73, 1740
Bsa29I ATCGAT 1 cut(s) 930
BsaAI YACGTR 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 930
BsaJI CCNNGG 7 cut(s) 265, 581, 633, 741, 2230, 2379, 2380
BsaWI WCCGGW 2 cut(s) 53, 860
Bsc4I CCNNNNNNNGG 5 cut(s) 551, 599, 693, 1745, 2113
Bse118I RCCGGY 1 cut(s) 2069
Bse1I ACTGG 7 cut(s) 445, 1076, 1321, 1762, 1929, 1978, 2107
Bse3DI GCAATG 2 cut(s) 1541, 2199
Bse8I GATNNNNATC 1 cut(s) 930
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 8 cut(s) 63, 582, 669, 1428, 2031, 2181, 2231, 2381
BseCI ATCGAT 1 cut(s) 930
BseDI CCNNGG 7 cut(s) 265, 581, 633, 741, 2230, 2379, 2380
BseGI GGATG 8 cut(s) 533, 661, 767, 799, 1042, 1177, 2343, 2375
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 5 cut(s) 551, 599, 693, 1745, 2113
BseMI GCAATG 2 cut(s) 1541, 2199
BseMII CTCAG 7 cut(s) 108, 702, 1254, 1586, 1868, 2172, 2342
BseNI ACTGG 7 cut(s) 445, 1076, 1321, 1762, 1929, 1978, 2107
BseSI GKGCMC 2 cut(s) 589, 1239
BseYI CCCAGC 2 cut(s) 1547, 2268
BsgI GTGCAG 3 cut(s) 97, 1407, 2046
Bsh1236I CGCG 2 cut(s) 222, 267
BshFI GGCC 6 cut(s) 649, 847, 890, 1152, 1322, 2397
BshNI GGYRCC 2 cut(s) 577, 586
BshVI ATCGAT 1 cut(s) 930
BsiHKAI GWGCWC 1 cut(s) 1051
BsiHKCI CYCGRG 3 cut(s) 451, 959, 2187
BsiSI CCGG 5 cut(s) 54, 73, 861, 1740, 2070
BslFI GGGAC 6 cut(s) 55, 247, 1650, 1830, 2326, 2384
BslI CCNNNNNNNGG 5 cut(s) 551, 599, 693, 1745, 2113
BsmAI GTCTC 3 cut(s) 943, 1263, 2418
BsmBI CGTCTC 1 cut(s) 1263
BsmFI GGGAC 6 cut(s) 55, 247, 1650, 1830, 2326, 2384
BsmI GAATGC 3 cut(s) 1401, 1880, 2025
BsnI GGCC 6 cut(s) 649, 847, 890, 1152, 1322, 2397
BsoBI CYCGRG 3 cut(s) 451, 959, 2187
Bsp1286I GDGCHC 3 cut(s) 589, 1051, 1239
Bsp13I TCCGGA 1 cut(s) 53
BspANI GGCC 6 cut(s) 649, 847, 890, 1152, 1322, 2397
BspCNI CTCAG 7 cut(s) 109, 703, 1253, 1585, 1867, 2171, 2341
BspDI ATCGAT 1 cut(s) 930
BspEI TCCGGA 1 cut(s) 53
BspFNI CGCG 2 cut(s) 222, 267
BspHI TCATGA 1 cut(s) 202
BspMAI CTGCAG 2 cut(s) 598, 1933
BspMI ACCTGC 6 cut(s) 382, 401, 409, 709, 1688, 1771
BspPI GGATC 4 cut(s) 188, 334, 926, 1596
BspQI GCTCTTC 2 cut(s) 206, 1773
BspT107I GGYRCC 2 cut(s) 577, 586
BspTI CTTAAG 1 cut(s) 140
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 2 cut(s) 1541, 2199
BsrFI RCCGGY 1 cut(s) 2069
BsrI ACTGG 7 cut(s) 445, 1076, 1321, 1762, 1929, 1978, 2107
BssAI RCCGGY 1 cut(s) 2069
BssECI CCNNGG 7 cut(s) 265, 581, 633, 741, 2230, 2379, 2380
BssT1I CCWWGG 1 cut(s) 633
Bst2UI CCWGG 8 cut(s) 63, 582, 669, 1428, 2031, 2181, 2231, 2381
Bst4CI ACNGT 6 cut(s) 476, 1216, 1557, 1944, 2120, 2225
Bst6I CTCTTC 3 cut(s) 206, 1714, 1773
BstAFI CTTAAG 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 593
BstBAI YACGTR 1 cut(s) 320
BstC8I GCNNGC 8 cut(s) 433, 1121, 1527, 1890, 1973, 2199, 2212, 2250
BstDEI CTNAG 9 cut(s) 117, 627, 711, 1240, 1572, 1854, 2158, 2328, 2406
BstDSI CCRYGG 2 cut(s) 265, 741
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 8 cut(s) 533, 661, 767, 799, 1042, 1177, 2343, 2375
BstFNI CGCG 2 cut(s) 222, 267
BstMAI GTCTC 3 cut(s) 943, 1263, 2418
BstNI CCWGG 8 cut(s) 63, 582, 669, 1428, 2031, 2181, 2231, 2381
BstNSI RCATGY 5 cut(s) 682, 764, 956, 2167, 2252
BstSFI CTRYAG 3 cut(s) 594, 1097, 1929
BstSLI GKGCMC 2 cut(s) 589, 1239
BstUI CGCG 2 cut(s) 222, 267
BstV2I GAAGAC 2 cut(s) 173, 1065
BstX2I RGATCY 3 cut(s) 126, 193, 1588
BstYI RGATCY 3 cut(s) 126, 193, 1588
Bsu15I ATCGAT 1 cut(s) 930
BsuI GTATCC 1 cut(s) 596
BsuRI GGCC 6 cut(s) 649, 847, 890, 1152, 1322, 2397
BsuTUI ATCGAT 1 cut(s) 930
BtgI CCRYGG 2 cut(s) 265, 741
BtgZI GCGATG 2 cut(s) 603, 2262
BtsCI GGATG 8 cut(s) 533, 661, 767, 799, 1042, 1177, 2343, 2375
BtsIMutI CAGTG 8 cut(s) 114, 481, 824, 1005, 1181, 1356, 1553, 1922
BveI ACCTGC 6 cut(s) 382, 401, 409, 709, 1688, 1771
Cac8I GCNNGC 8 cut(s) 433, 1121, 1527, 1890, 1973, 2199, 2212, 2250
CaiI CAGNNNCTG 2 cut(s) 1004, 1924
CciI TCATGA 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 2069
Cfr42I CCGCGG 1 cut(s) 268
ClaI ATCGAT 1 cut(s) 930
CseI GACGC 1 cut(s) 2381
CsiI ACCWGGT 2 cut(s) 61, 2179
Csp6I GTAC 3 cut(s) 379, 2110, 2171
CviQI GTAC 3 cut(s) 379, 2110, 2171
DdeI CTNAG 9 cut(s) 117, 627, 711, 1240, 1572, 1854, 2158, 2328, 2406
DrdI GACNNNNNNGTC 1 cut(s) 2338
DseDI GACNNNNNNGTC 1 cut(s) 2338
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 3 cut(s) 206, 1714, 1773
EarI CTCTTC 3 cut(s) 206, 1714, 1773
EciI GGCGGA 3 cut(s) 36, 339, 2249
Eco130I CCWWGG 1 cut(s) 633
Eco32I GATATC 2 cut(s) 557, 928
Eco47I GGWCC 6 cut(s) 69, 157, 261, 1664, 1760, 2177
Eco57I CTGAAG 4 cut(s) 1077, 1332, 1407, 2123
Eco72I CACGTG 1 cut(s) 320
Eco88I CYCGRG 3 cut(s) 451, 959, 2187
EcoNI CCTNNNNNAGG 1 cut(s) 597
EcoO109I RGGNCCY 2 cut(s) 69, 261
EcoRI GAATTC 1 cut(s) 613
EcoRII CCWGG 8 cut(s) 61, 580, 667, 1426, 2029, 2179, 2229, 2379
EcoRV GATATC 2 cut(s) 557, 928
EcoT14I CCWWGG 1 cut(s) 633
EcoT22I ATGCAT 1 cut(s) 898
ErhI CCWWGG 1 cut(s) 633
Esp3I CGTCTC 1 cut(s) 1263
FaqI GGGAC 6 cut(s) 55, 247, 1650, 1830, 2326, 2384
FauI CCCGC 2 cut(s) 272, 2206
FbaI TGATCA 1 cut(s) 1393
FblI GTMKAC 2 cut(s) 370, 1807
FokI GGATG 8 cut(s) 520, 648, 754, 806, 1049, 1164, 2350, 2362
FspBI CTAG 7 cut(s) 258, 512, 732, 776, 1248, 1803, 2022
GsaI CCCAGC 2 cut(s) 1551, 2272
GsuI CTGGAG 5 cut(s) 651, 1095, 1350, 2052, 2313
HaeIII GGCC 6 cut(s) 649, 847, 890, 1152, 1322, 2397
HapII CCGG 5 cut(s) 54, 73, 861, 1740, 2070
HgaI GACGC 1 cut(s) 2381
HindIII AAGCTT 1 cut(s) 1890
HinfI GANTC 6 cut(s) 254, 350, 479, 1340, 2050, 2239
HpaII CCGG 5 cut(s) 54, 73, 861, 1740, 2070
HphI GGTGA 5 cut(s) 314, 992, 1085, 1466, 1593
Hpy166II GTNNAC 4 cut(s) 371, 1487, 1808, 2173
Hpy188III TCNNGA 7 cut(s) 54, 191, 203, 552, 1112, 1256, 2187
Hpy8I GTNNAC 4 cut(s) 371, 1487, 1808, 2173
Hpy99I CGWCG 1 cut(s) 462
HpyCH4III ACNGT 6 cut(s) 476, 1216, 1557, 1944, 2120, 2225
HpyCH4IV ACGT 2 cut(s) 319, 1644
HpyF3I CTNAG 9 cut(s) 117, 627, 711, 1240, 1572, 1854, 2158, 2328, 2406
HpySE526I ACGT 2 cut(s) 319, 1644
KflI GGGWCCC 1 cut(s) 69
Kpn2I TCCGGA 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 1393
KspI CCGCGG 1 cut(s) 268
LguI GCTCTTC 2 cut(s) 206, 1773
LmnI GCTCC 5 cut(s) 1046, 1107, 1510, 2108, 2294
MabI ACCWGGT 2 cut(s) 61, 2179
MaeI CTAG 7 cut(s) 258, 512, 732, 776, 1248, 1803, 2022
MaeII ACGT 2 cut(s) 319, 1644
MaeIII GTNAC 9 cut(s) 149, 502, 763, 1190, 1612, 1901, 1920, 2120, 2133
MbiI CCGCTC 1 cut(s) 6
MflI RGATCY 3 cut(s) 126, 193, 1588
MhlI GDGCHC 3 cut(s) 589, 1051, 1239
MluCI AATT 8 cut(s) 274, 283, 613, 706, 724, 2215, 2285, 2420
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 1235, 1851
Mph1103I ATGCAT 1 cut(s) 898
MroI TCCGGA 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 529
MseI TTAA 2 cut(s) 141, 1370
MslI CAYNNNNRTG 3 cut(s) 1242, 1676, 1868
MspA1I CMGCKG 4 cut(s) 267, 491, 1662, 2201
MspCI CTTAAG 1 cut(s) 140
MspI CCGG 5 cut(s) 54, 73, 861, 1740, 2070
Mva1269I GAATGC 3 cut(s) 1401, 1880, 2025
MvaI CCWGG 8 cut(s) 63, 582, 669, 1428, 2031, 2181, 2231, 2381
MvnI CGCG 2 cut(s) 222, 267
NciI CCSGG 2 cut(s) 73, 1740
NmeAIII GCCGAG 1 cut(s) 866
NmuCI GTSAC 5 cut(s) 502, 1190, 1612, 1920, 2133
NsiI ATGCAT 1 cut(s) 898
NspI RCATGY 5 cut(s) 682, 764, 956, 2167, 2252
PaeI GCATGC 1 cut(s) 2252
PaeR7I CTCGAG 2 cut(s) 451, 959
PagI TCATGA 1 cut(s) 202
PaqCI CACCTGC 2 cut(s) 409, 1688
PasI CCCWGGG 1 cut(s) 2380
PciI ACATGT 1 cut(s) 952
PciSI GCTCTTC 2 cut(s) 206, 1773
PctI GAATGC 3 cut(s) 1401, 1880, 2025
PdmI GAANNNNTTC 1 cut(s) 529
PfeI GAWTC 5 cut(s) 254, 479, 1340, 2050, 2239
PfoI TCCNGGA 2 cut(s) 71, 2029
PleI GAGTC 1 cut(s) 358
PmaCI CACGTG 1 cut(s) 320
PmlI CACGTG 1 cut(s) 320
PpsI GAGTC 1 cut(s) 358
Ppu21I YACGTR 1 cut(s) 320
PpuMI RGGWCCY 2 cut(s) 69, 261
PscI ACATGT 1 cut(s) 952
PsiI TTATAA 1 cut(s) 2099
Psp5II RGGWCCY 2 cut(s) 69, 261
Psp6I CCWGG 8 cut(s) 61, 580, 667, 1426, 2029, 2179, 2229, 2379
PspCI CACGTG 1 cut(s) 320
PspFI CCCAGC 2 cut(s) 1547, 2268
PspGI CCWGG 8 cut(s) 61, 580, 667, 1426, 2029, 2179, 2229, 2379
PspPPI RGGWCCY 2 cut(s) 69, 261
PspXI VCTCGAGB 2 cut(s) 451, 959
PstI CTGCAG 2 cut(s) 598, 1933
PstNI CAGNNNCTG 2 cut(s) 1004, 1924
PsuI RGATCY 3 cut(s) 126, 193, 1588
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 3 cut(s) 380, 2111, 2172
RsaNI GTAC 3 cut(s) 379, 2110, 2171
RseI CAYNNNNRTG 3 cut(s) 1242, 1676, 1868
SacII CCGCGG 1 cut(s) 268
SapI GCTCTTC 2 cut(s) 206, 1773
SaqAI TTAA 2 cut(s) 141, 1370
SchI GAGTC 1 cut(s) 359
SduI GDGCHC 3 cut(s) 589, 1051, 1239
SexAI ACCWGGT 2 cut(s) 61, 2179
SfcI CTRYAG 3 cut(s) 594, 1097, 1929
Sfr274I CTCGAG 2 cut(s) 451, 959
Sfr303I CCGCGG 1 cut(s) 268
SgrBI CCGCGG 1 cut(s) 268
SinI GGWCC 6 cut(s) 69, 157, 261, 1664, 1760, 2177
SlaI CTCGAG 2 cut(s) 451, 959
SmiMI CAYNNNNRTG 3 cut(s) 1242, 1676, 1868
SmlI CTYRAG 5 cut(s) 140, 189, 451, 959, 1126
SmoI CTYRAG 5 cut(s) 140, 189, 451, 959, 1126
SphI GCATGC 1 cut(s) 2252
Sse9I AATT 8 cut(s) 274, 283, 613, 706, 724, 2215, 2285, 2420
SspMI CTAG 7 cut(s) 258, 512, 732, 776, 1248, 1803, 2022
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 6 cut(s) 476, 1216, 1557, 1944, 2120, 2225
TaiI ACGT 2 cut(s) 322, 1647
TaqI TCGA 4 cut(s) 452, 930, 960, 2346
TasI AATT 8 cut(s) 274, 283, 613, 706, 724, 2215, 2285, 2420
TatI WGTACW 1 cut(s) 2109
TauI GCSGC 3 cut(s) 18, 21, 431
TfiI GAWTC 5 cut(s) 254, 479, 1340, 2050, 2239
Tru1I TTAA 2 cut(s) 141, 1370
Tru9I TTAA 2 cut(s) 141, 1370
TscAI CASTG 8 cut(s) 121, 481, 831, 1005, 1188, 1356, 1560, 1929
TseFI GTSAC 5 cut(s) 502, 1190, 1612, 1920, 2133
Tsp45I GTSAC 5 cut(s) 502, 1190, 1612, 1920, 2133
TspDTI ATGAA 9 cut(s) 180, 191, 219, 522, 606, 717, 984, 984, 1298
TspGWI ACGGA 6 cut(s) 266, 805, 806, 815, 2034, 2190
TspRI CASTG 8 cut(s) 121, 481, 831, 1005, 1188, 1356, 1560, 1929
Vha464I CTTAAG 1 cut(s) 140
VpaK11BI GGWCC 6 cut(s) 69, 157, 261, 1664, 1760, 2177
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 2 cut(s) 613, 706
XceI RCATGY 5 cut(s) 682, 764, 956, 2167, 2252
XcmI CCANNNNNNNNNTGG 1 cut(s) 1555
XhoI CTCGAG 2 cut(s) 451, 959
XmiI GTMKAC 2 cut(s) 370, 1807
XmnI GAANNNNTTC 1 cut(s) 529
XspI CTAG 7 cut(s) 258, 512, 732, 776, 1248, 1803, 2022
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.