MD10G1266800.v1.1

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006562
Physical Location & Seq
Reverse (-)
35979340 .. 35985832
6493 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1266800.v1.1.491

Sequence Viewer

Length: 2571 bp
ATGAGCGGGAGAGGCGGCGGAGGCGTGGCCAGCAATGGCAAGGGCAATACTGGGATTTCGGGCATACCGGCGGGATCTCGGAAGATGGTACAGAGCTTGAAGGAGATTGTGAATAACTGCACTGAGCAGGAGATCTACGCTATGCTTAAAGACTGTAACATGGACCCTAACGAAGCCGTTAATCGCCTCCTGGCCCAAGATCCTTTTCATGAAGTGAGGAGCAAACGAGAAAAGAAAAAAGAGAGTAAGGAACCGACGGACTCCAGGTCCCGTGGGGCGAATAACACTTCAAGTTATGGTGCTAGAGGTGGTGACCGTTATTCTGCACGTGGCGGATCAAACCACTATAGCTCTAATGATTCTGGTTTTTCGCATGGTAAGCCTGCATACAAGAAGGAAAATGGAACACATGCTTATCCAGGTTCTGCATCTGTCATGGCGGGACATAACACAGGTCGACGACCTACATCCTACAGCGATTCTGTGGGGAGTGAAAATAAAATCCCAGCACTAAGCACAGATGATGGAATATTGTCATCCTCACAGCCTTCTGCAGGATATCAGTCTGCATGGGTAGGGGTTCCGGGTCAAGTTTCCATGGCCGACATTGTTAAGATGGGCAGGCCGCAAGCCAAGGCTTCCACCGTGCCAAACCCCCATAATCATAGTGGTAATAATCAAGATGGGGCACCTGCAACATCATTGCATCACAATTTGCGTTCACCACAAGATCATGTTCCAAAGTTGCCAGCAACCAATGATGAGTGGCCCTTGATTGATCCACCTTCTGTTAGCATGTCCTCTGTTCTTGGAGCACCCACAAACTCTGAGCTGTATGCTGATTCATCTAACTTGCCCATCGATAGAACTAGTCAACATATAAGAGCCCAGCTAGATGAGGTCGAGGTGGAAGAAGATGATTCTGTTGAGGCACTTCCAAGCCATAATGAACCTACTTCTGTATCCGGTAGACATATACAAGAAGATAATTCTGGAGGTGCATCTGCTTTTGATAATAACTTGTATGAAGACATAAATTCCTTCCAACCACAGAGGCATGCTTTCGAGGATAATGAAGCTGAAGAAGATGTGTCATCAGTGGCTGCAAACTTGCAGCAACTTAATTTACAGAACGATGATGGGGGAGCAGCTCCTGAAGAAGACAAACCTCATGTGGTCATTCCTAATCACCTTCAGCTTCATACTCCAGATGTCTTGAACTTGAGCTTTGGAAGTTTCCGATCTGGCCCTGATCCTGCTCTTCCTAGCTCCGAATCATTTGCATCTGAGCCACAAAAAAGTAACTTTGAAGAGACATCTGGGGCCGTAGATGTTTCTGCAATTGCACACTCAGATTCAAGAAATCCTGAGTATTATGGAGATGAGCATCTCATGAATGCCTCTGATGGAAATCTAGTTCATAGAACGGTAGCCAGTGCTGGAGATTATGAATCTCCTTCGGTTTCACAAGCAGAGATCTTGAAACAAGAAACCCATGAAGCTGGTCAAGGGAATCAATATGTGTTCCCTTCTGCACCTGGATTTGCATACGAGAACTCCCAACAATTGGATGCGACATTTCCTCACCAACAGACAAGCTCACAGATGCAGAATATTGCTCCTTTTTCGAATGTGATGGCATATACAAATTCATTGCCCAGCACTCTGTTGGCTTCGACTGCTCAAGCTGCAAGAGAGGATTTTCCATACTCAGCGTTCCCCATGACCCAATCTATGCCCACAAATTATGGCAATGCGGCTTCTTCTATCAGCGGTCCCACCATCTCCATGACAGAGGCATTGAGAGCAGGTGGTATTTCTGGAGCTCAGCCTACTCCACAGACCCTGCCTGGTGCTAATGTTGCAACTGGACCAGCTCTTCCACAACACCTTGCAATGCACCCTTATTCTCAACCTACTCTTCCACTGGGGCATTTTTCAAACATGATCGGTTATCCTTTCCTGCCTCAAAGCTACACATATATGCCGTCAGCTTTCCAGCAACAATTTGCTGGTAACAGCACATACCATCAGTCTCTTGCTGCAGTTCTCCCACAGTATAAAAATAGTGTCTCTGCTAGCAGTTTGCCTCAGTCTGCTAATATTCCGCCCGGTTATGGGTTCAGTAGTTCAGCCAGCATTCCTGGTGGAAACTTTCCTTTGAATCCATCCTCGGCACCTACAGGCACAACGATTGGCTATGACGATGTTGTAAACTCTCAGTTCAAGGACAATAGCCATTTGATCTCACTTCAGCAGCAACAAAATGATAACTCGGGAATGTGGGTTCATGGACCCGGTTCCCGAGCAATGTCCGCTGTTCCAGCCAGCAACTATTACAGCTTCCAGGGGCAAAACCAGCAGCACACAGGGTTCCGGCAAGCTCAGCAACCACAACCTTCACAGCAATATGCTGGAGCTCTCGGTTACCCCAACTTTTACCACTCTCAATCCGGGATGTCCCTAGAGCACCAGCAGCAGCAAAGTTCAAGGGACGCGTCCCTTGGCGGCTCACAAGGCGGCCAACCCTCGAAGCAGTCGCAACAGCATTTATGGCAGAACAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

857

Amino Acids

91.47

Weight (kDa)

5.32

Isoelectric Point (pI)

61.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 22 - 81 1.8e-33 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 700, 1799
Acc36I ACCTGC 2 cut(s) 700, 1799
AccB1I GGYRCC 2 cut(s) 688, 2176
AccB7I CCANNNNNTGG 1 cut(s) 1567
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 2 cut(s) 457, 970
AccII CGCG 1 cut(s) 2498
AclWI GGATC 5 cut(s) 82, 194, 343, 773, 1247
AcoI YGGCCR 3 cut(s) 27, 600, 2521
AcsI RAATTY 2 cut(s) 1036, 1648
AcuI CTGAAG 4 cut(s) 1101, 1176, 1178, 2237
AcvI CACGTG 1 cut(s) 329
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 4 cut(s) 554, 1567, 2117, 2118
AflIII ACRYGT 1 cut(s) 2496
AhdI GACNNNNNGTC 1 cut(s) 265
AhlI ACTAGT 1 cut(s) 869
AjnI CCWGG 7 cut(s) 189, 263, 418, 1537, 1849, 2143, 2346
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AloI GAACNNNNNNTCC 4 cut(s) 1401, 1433, 2271, 2303
Alw21I GWGCWC 4 cut(s) 817, 1828, 2422, 2472
Alw26I GTCTC 3 cut(s) 1307, 2040, 2077
AlwI GGATC 5 cut(s) 82, 194, 343, 773, 1247
AlwNI CAGNNNCTG 4 cut(s) 425, 1103, 1154, 1846
Ama87I CYCGRG 2 cut(s) 2275, 2304
AoxI GGCC 8 cut(s) 27, 192, 600, 623, 767, 1246, 1323, 2521
ApeKI GCWGC 9 cut(s) 1103, 1114, 1148, 1688, 2042, 2257, 2362, 2476, 2479
ApoI RAATTY 2 cut(s) 1036, 1648
AspS9I GGNCC 9 cut(s) 163, 193, 267, 768, 1247, 1323, 1775, 1871, 2294
AsuC2I CCSGG 4 cut(s) 585, 2112, 2298, 2455
AsuHPI GGTGA 4 cut(s) 323, 714, 1181, 1577
AsuII TTCGAA 1 cut(s) 1628
AsuNHI GCTAGC 1 cut(s) 2078
AvaI CYCGRG 2 cut(s) 2275, 2304
AvaII GGWCC 5 cut(s) 163, 267, 1775, 1871, 2294
BaeGI GKGCMC 1 cut(s) 691
BaeI ACNNNNGTAYC 2 cut(s) 945, 978
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 2 cut(s) 688, 2176
BanII GRGCYC 3 cut(s) 889, 1828, 2422
BbrPI CACGTG 1 cut(s) 329
BbsI GAAGAC 2 cut(s) 1035, 1167
Bbv12I GWGCWC 4 cut(s) 817, 1828, 2422, 2472
BbvI GCAGC 9 cut(s) 1090, 1126, 1160, 1675, 2029, 2269, 2374, 2488, 2491
BceAI ACGGC 3 cut(s) 161, 1310, 1972
BcgI CGANNNNNNTGC 2 cut(s) 1262, 1296
BciT130I CCWGG 7 cut(s) 191, 265, 420, 1539, 1851, 2145, 2348
BciVI GTATCC 1 cut(s) 973
BcnI CCSGG 4 cut(s) 585, 2112, 2298, 2455
BcoDI GTCTC 3 cut(s) 1307, 2040, 2077
BcuI ACTAGT 1 cut(s) 869
BfaI CTAG 7 cut(s) 303, 870, 893, 1266, 1415, 2079, 2465
BfmI CTRYAG 5 cut(s) 346, 472, 552, 2043, 2181
BfuAI ACCTGC 2 cut(s) 700, 1799
BfuI GTATCC 1 cut(s) 973
BglII AGATCT 2 cut(s) 132, 1476
BlpI GCTNAGC 2 cut(s) 1827, 2385
Bme18I GGWCC 5 cut(s) 163, 267, 1775, 1871, 2294
BmeRI GACNNNNNGTC 1 cut(s) 265
BmeT110I CYCGRG 2 cut(s) 2275, 2304
BmgT120I GGNCC 9 cut(s) 163, 193, 267, 768, 1247, 1323, 1775, 1871, 2294
BmrI ACTGGG 2 cut(s) 60, 1937
BmsI GCATC 7 cut(s) 437, 715, 1010, 1292, 1396, 1561, 1596
BmtI GCTAGC 1 cut(s) 2082
BmuI ACTGGG 2 cut(s) 60, 1937
BpiI GAAGAC 2 cut(s) 1035, 1167
BpmI CTGGAG 6 cut(s) 247, 1014, 1191, 1461, 1842, 2436
Bpu1102I GCTNAGC 2 cut(s) 1827, 2385
Bpu14I TTCGAA 1 cut(s) 1628
BpuEI CTTGAG 2 cut(s) 1243, 1668
BpuMI CCSGG 4 cut(s) 585, 2112, 2298, 2455
Bsa29I ATCGAT 1 cut(s) 861
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 2 cut(s) 1386, 1410
BsaJI CCNNGG 6 cut(s) 271, 597, 633, 2172, 2347, 2503
BsaWI WCCGGW 1 cut(s) 965
BsaXI ACNNNNNCTCC 2 cut(s) 1541, 1571
Bsc4I CCNNNNNNNGG 4 cut(s) 554, 1567, 2117, 2118
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 4 cut(s) 55, 1434, 1873, 1932
Bse3DI GCAATG 6 cut(s) 40, 701, 1652, 1759, 1902, 2316
Bse8I GATNNNNATC 2 cut(s) 1386, 1410
BseBI CCWGG 7 cut(s) 191, 265, 420, 1539, 1851, 2145, 2348
BseCI ATCGAT 1 cut(s) 861
BseDI CCNNGG 6 cut(s) 271, 597, 633, 2172, 2347, 2503
BseGI GGATG 5 cut(s) 467, 536, 1576, 2168, 2463
BseJI GATNNNNATC 2 cut(s) 1386, 1410
BseLI CCNNNNNNNGG 4 cut(s) 554, 1567, 2117, 2118
BseMI GCAATG 6 cut(s) 40, 701, 1652, 1759, 1902, 2316
BseNI ACTGG 4 cut(s) 55, 1434, 1873, 1932
BseRI GAGGAG 1 cut(s) 232
BseSI GKGCMC 1 cut(s) 691
BseXI GCAGC 9 cut(s) 1090, 1126, 1160, 1675, 2029, 2269, 2374, 2488, 2491
BseYI CCCAGC 3 cut(s) 505, 888, 1658
BsgI GTGCAG 3 cut(s) 103, 309, 1518
Bsh1236I CGCG 1 cut(s) 2498
BshFI GGCC 8 cut(s) 29, 194, 602, 625, 769, 1248, 1325, 2523
BshNI GGYRCC 2 cut(s) 688, 2176
BshVI ATCGAT 1 cut(s) 861
BsiHKAI GWGCWC 4 cut(s) 817, 1828, 2422, 2472
BsiHKCI CYCGRG 2 cut(s) 2275, 2304
BsiSI CCGG 7 cut(s) 68, 584, 966, 2112, 2298, 2377, 2454
BslFI GGGAC 6 cut(s) 253, 456, 1761, 2446, 2485, 2507
BslI CCNNNNNNNGG 4 cut(s) 554, 1567, 2117, 2118
BsmAI GTCTC 3 cut(s) 1307, 2040, 2077
BsmFI GGGAC 6 cut(s) 253, 456, 1761, 2446, 2485, 2507
BsmI GAATGC 2 cut(s) 1402, 2139
BsnI GGCC 8 cut(s) 29, 194, 602, 625, 769, 1248, 1325, 2523
BsoBI CYCGRG 2 cut(s) 2275, 2304
Bsp119I TTCGAA 1 cut(s) 1628
Bsp1286I GDGCHC 6 cut(s) 691, 817, 889, 1828, 2422, 2472
Bsp1720I GCTNAGC 2 cut(s) 1827, 2385
Bsp19I CCATGG 1 cut(s) 597
BspANI GGCC 8 cut(s) 29, 194, 602, 625, 769, 1248, 1325, 2523
BspDI ATCGAT 1 cut(s) 861
BspFNI CGCG 1 cut(s) 2498
BspHI TCATGA 2 cut(s) 208, 1392
BspMAI CTGCAG 2 cut(s) 556, 2047
BspMI ACCTGC 2 cut(s) 700, 1799
BspOI GCTAGC 1 cut(s) 2082
BspPI GGATC 5 cut(s) 82, 194, 343, 773, 1247
BspQI GCTCTTC 2 cut(s) 1266, 1884
BspT104I TTCGAA 1 cut(s) 1628
BspT107I GGYRCC 2 cut(s) 688, 2176
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 6 cut(s) 40, 701, 1652, 1759, 1902, 2316
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 4 cut(s) 55, 1434, 1873, 1932
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 6 cut(s) 271, 597, 633, 2172, 2347, 2503
BssT1I CCWWGG 3 cut(s) 597, 633, 2503
Bst2UI CCWGG 7 cut(s) 191, 265, 420, 1539, 1851, 2145, 2348
Bst4CI ACNGT 5 cut(s) 155, 317, 646, 1429, 2058
Bst6I CTCTTC 4 cut(s) 1266, 1305, 1884, 1926
BstBAI YACGTR 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 1628
BstDSI CCRYGG 2 cut(s) 271, 597
BstEII GGTNACC 2 cut(s) 311, 2426
BstENI CCTNNNNNAGG 1 cut(s) 552
BstF5I GGATG 5 cut(s) 467, 536, 1576, 2168, 2463
BstFNI CGCG 1 cut(s) 2498
BstMAI GTCTC 3 cut(s) 1307, 2040, 2077
BstNI CCWGG 7 cut(s) 191, 265, 420, 1539, 1851, 2145, 2348
BstNSI RCATGY 3 cut(s) 413, 799, 1061
BstPI GGTNACC 2 cut(s) 311, 2426
BstSFI CTRYAG 5 cut(s) 346, 472, 552, 2043, 2181
BstSLI GKGCMC 1 cut(s) 691
BstUI CGCG 1 cut(s) 2498
BstV1I GCAGC 9 cut(s) 1090, 1126, 1160, 1675, 2029, 2269, 2374, 2488, 2491
BstV2I GAAGAC 2 cut(s) 1035, 1167
BstX2I RGATCY 4 cut(s) 74, 132, 199, 1476
BstXI CCANNNNNNTGG 1 cut(s) 1502
BstYI RGATCY 4 cut(s) 74, 132, 199, 1476
Bsu15I ATCGAT 1 cut(s) 861
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 8 cut(s) 29, 194, 602, 625, 769, 1248, 1325, 2523
BsuTUI ATCGAT 1 cut(s) 861
BtgI CCRYGG 2 cut(s) 271, 597
BtsCI GGATG 5 cut(s) 467, 536, 1576, 2168, 2463
BtsIMutI CAGTG 4 cut(s) 120, 1104, 1441, 1925
BveI ACCTGC 2 cut(s) 700, 1799
CaiI CAGNNNCTG 4 cut(s) 425, 1103, 1154, 1846
CciI TCATGA 2 cut(s) 208, 1392
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 9 cut(s) 163, 193, 267, 768, 1247, 1323, 1775, 1871, 2294
ClaI ATCGAT 1 cut(s) 861
CseI GACGC 2 cut(s) 2487, 2504
Csp6I GTAC 1 cut(s) 89
CviQI GTAC 1 cut(s) 89
DriI GACNNNNNGTC 1 cut(s) 265
EaeI YGGCCR 3 cut(s) 27, 600, 2521
Eam1104I CTCTTC 4 cut(s) 1266, 1305, 1884, 1926
Eam1105I GACNNNNNGTC 1 cut(s) 265
EarI CTCTTC 4 cut(s) 1266, 1305, 1884, 1926
EciI GGCGGA 3 cut(s) 33, 348, 2097
Ecl136II GAGCTC 2 cut(s) 1826, 2420
Eco130I CCWWGG 3 cut(s) 597, 633, 2503
Eco24I GRGCYC 3 cut(s) 889, 1828, 2422
Eco32I GATATC 1 cut(s) 560
Eco47I GGWCC 5 cut(s) 163, 267, 1775, 1871, 2294
Eco53kI GAGCTC 2 cut(s) 1826, 2420
Eco57I CTGAAG 4 cut(s) 1101, 1176, 1178, 2237
Eco72I CACGTG 1 cut(s) 329
Eco88I CYCGRG 2 cut(s) 2275, 2304
Eco91I GGTNACC 2 cut(s) 311, 2426
EcoICRI GAGCTC 2 cut(s) 1826, 2420
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 267
EcoO65I GGTNACC 2 cut(s) 311, 2426
EcoRII CCWGG 7 cut(s) 189, 263, 418, 1537, 1849, 2143, 2346
EcoRV GATATC 1 cut(s) 560
EcoT14I CCWWGG 3 cut(s) 597, 633, 2503
EcoT38I GRGCYC 3 cut(s) 889, 1828, 2422
ErhI CCWWGG 3 cut(s) 597, 633, 2503
FaqI GGGAC 6 cut(s) 253, 456, 1761, 2446, 2485, 2507
FauI CCCGC 2 cut(s) 64, 433
FblI GTMKAC 2 cut(s) 457, 970
FokI GGATG 5 cut(s) 454, 523, 1583, 2155, 2470
FriOI GRGCYC 3 cut(s) 889, 1828, 2422
FspBI CTAG 7 cut(s) 303, 870, 893, 1266, 1415, 2079, 2465
GsaI CCCAGC 3 cut(s) 509, 892, 1662
GsuI CTGGAG 6 cut(s) 247, 1014, 1191, 1461, 1842, 2436
HaeIII GGCC 8 cut(s) 29, 194, 602, 625, 769, 1248, 1325, 2523
HapII CCGG 7 cut(s) 68, 584, 966, 2112, 2298, 2377, 2454
HgaI GACGC 2 cut(s) 2487, 2504
HincII GTYRAC 2 cut(s) 458, 875
HindII GTYRAC 2 cut(s) 458, 875
HpaII CCGG 7 cut(s) 68, 584, 966, 2112, 2298, 2377, 2454
HphI GGTGA 4 cut(s) 323, 714, 1181, 1577
Hpy166II GTNNAC 5 cut(s) 458, 722, 875, 971, 2215
Hpy188I TCNGA 7 cut(s) 81, 829, 1241, 1273, 1288, 1354, 1405
Hpy8I GTNNAC 5 cut(s) 458, 722, 875, 971, 2215
Hpy99I CGWCG 2 cut(s) 259, 462
HpyAV CCTTC 9 cut(s) 94, 388, 558, 795, 1051, 1202, 1467, 1539, 2409
HpyCH4III ACNGT 5 cut(s) 155, 317, 646, 1429, 2058
HpyCH4IV ACGT 1 cut(s) 328
HpySE526I ACGT 1 cut(s) 328
LguI GCTCTTC 2 cut(s) 1266, 1884
LmnI GCTCC 8 cut(s) 219, 812, 1145, 1156, 1274, 1624, 1823, 2417
Lsp1109I GCAGC 9 cut(s) 1090, 1126, 1160, 1675, 2029, 2269, 2374, 2488, 2491
LweI GCATC 7 cut(s) 437, 715, 1010, 1292, 1396, 1561, 1596
MaeI CTAG 7 cut(s) 303, 870, 893, 1266, 1415, 2079, 2465
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 5 cut(s) 155, 311, 1301, 2015, 2426
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 2 cut(s) 1341, 1565
MflI RGATCY 4 cut(s) 74, 132, 199, 1476
MhlI GDGCHC 6 cut(s) 691, 817, 889, 1828, 2422, 2472
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 9 cut(s) 712, 988, 1036, 1123, 1341, 1565, 1648, 1744, 2006
MluI ACGCGT 1 cut(s) 2496
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 1069
Mox20I TGGCCA 1 cut(s) 29
MscI TGGCCA 1 cut(s) 29
MseI TTAA 4 cut(s) 147, 180, 612, 1122
MslI CAYNNNNRTG 2 cut(s) 1787, 1982
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 2 cut(s) 1773, 2318
MspI CCGG 7 cut(s) 68, 584, 966, 2112, 2298, 2377, 2454
MunI CAATTG 2 cut(s) 1341, 1565
Mva1269I GAATGC 2 cut(s) 1402, 2139
MvaI CCWGG 7 cut(s) 191, 265, 420, 1539, 1851, 2145, 2348
MvnI CGCG 1 cut(s) 2498
NciI CCSGG 4 cut(s) 585, 2112, 2298, 2455
NcoI CCATGG 1 cut(s) 597
NheI GCTAGC 1 cut(s) 2078
NmeAIII GCCGAG 1 cut(s) 2153
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 3 cut(s) 413, 799, 1061
NspV TTCGAA 1 cut(s) 1628
PaeI GCATGC 1 cut(s) 1061
PagI TCATGA 2 cut(s) 208, 1392
PaqCI CACCTGC 2 cut(s) 700, 1799
PciSI GCTCTTC 2 cut(s) 1266, 1884
PctI GAATGC 2 cut(s) 1402, 2139
PfeI GAWTC 9 cut(s) 359, 479, 842, 920, 1274, 1355, 1451, 1513, 2164
PflMI CCANNNNNTGG 1 cut(s) 1567
PfoI TCCNGGA 1 cut(s) 2453
PleI GAGTC 1 cut(s) 254
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 1 cut(s) 254
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 267
Psp124BI GAGCTC 2 cut(s) 1828, 2422
Psp5II RGGWCCY 1 cut(s) 267
Psp6I CCWGG 7 cut(s) 189, 263, 418, 1537, 1849, 2143, 2346
PspCI CACGTG 1 cut(s) 329
PspEI GGTNACC 2 cut(s) 311, 2426
PspFI CCCAGC 3 cut(s) 505, 888, 1658
PspGI CCWGG 7 cut(s) 189, 263, 418, 1537, 1849, 2143, 2346
PspPI GGNCC 9 cut(s) 163, 193, 267, 768, 1247, 1323, 1775, 1871, 2294
PspPPI RGGWCCY 1 cut(s) 267
PstI CTGCAG 2 cut(s) 556, 2047
PstNI CAGNNNCTG 4 cut(s) 425, 1103, 1154, 1846
PsuI RGATCY 4 cut(s) 74, 132, 199, 1476
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 2 cut(s) 1787, 1982
SacI GAGCTC 2 cut(s) 1828, 2422
SalI GTCGAC 1 cut(s) 456
SapI GCTCTTC 2 cut(s) 1266, 1884
SaqAI TTAA 4 cut(s) 147, 180, 612, 1122
Sau96I GGNCC 9 cut(s) 163, 193, 267, 768, 1247, 1323, 1775, 1871, 2294
SchI GAGTC 1 cut(s) 254
SduI GDGCHC 6 cut(s) 691, 817, 889, 1828, 2422, 2472
SfaNI GCATC 7 cut(s) 437, 715, 1010, 1292, 1396, 1561, 1596
SfcI CTRYAG 5 cut(s) 346, 472, 552, 2043, 2181
SfuI TTCGAA 1 cut(s) 1628
SinI GGWCC 5 cut(s) 163, 267, 1775, 1871, 2294
SmiMI CAYNNNNRTG 2 cut(s) 1787, 1982
SmlI CTYRAG 2 cut(s) 1222, 1683
SmoI CTYRAG 2 cut(s) 1222, 1683
SpeI ACTAGT 1 cut(s) 869
SphI GCATGC 1 cut(s) 1061
Sse9I AATT 9 cut(s) 712, 988, 1036, 1123, 1341, 1565, 1648, 1744, 2006
SspI AATATT 3 cut(s) 531, 1615, 2104
SspMI CTAG 7 cut(s) 303, 870, 893, 1266, 1415, 2079, 2465
SstI GAGCTC 2 cut(s) 1828, 2422
StyI CCWWGG 3 cut(s) 597, 633, 2503
TaaI ACNGT 5 cut(s) 155, 317, 646, 1429, 2058
TaiI ACGT 1 cut(s) 331
TaqI TCGA 7 cut(s) 457, 861, 903, 1065, 1628, 1676, 2531
TasI AATT 9 cut(s) 712, 988, 1036, 1123, 1341, 1565, 1648, 1744, 2006
TauI GCSGC 5 cut(s) 18, 628, 1760, 2511, 2523
TfiI GAWTC 9 cut(s) 359, 479, 842, 920, 1274, 1355, 1451, 1513, 2164
Tru1I TTAA 4 cut(s) 147, 180, 612, 1122
Tru9I TTAA 4 cut(s) 147, 180, 612, 1122
TscAI CASTG 4 cut(s) 127, 1104, 1441, 1932
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 9 cut(s) 1103, 1114, 1148, 1688, 2042, 2257, 2362, 2476, 2479
Tsp45I GTSAC 1 cut(s) 311
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 4 cut(s) 127, 1104, 1441, 1932
Van91I CCANNNNNTGG 1 cut(s) 1567
VpaK11BI GGWCC 5 cut(s) 163, 267, 1775, 1871, 2294
XagI CCTNNNNNAGG 1 cut(s) 552
XapI RAATTY 2 cut(s) 1036, 1648
XceI RCATGY 3 cut(s) 413, 799, 1061
XcmI CCANNNNNNNNNTGG 2 cut(s) 665, 1666
XmiI GTMKAC 2 cut(s) 457, 970
XspI CTAG 7 cut(s) 303, 870, 893, 1266, 1415, 2079, 2465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.