Rh5BG097100

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006562
Physical Location & Seq
Forward (+)
8794231 .. 8800168
5938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG097100.1

Sequence Viewer

Length: 2472 bp
ATGAGCGGGAGAGGCGGCGGCAATGGGAAGGGCAATACTGGAATATCGGGAATACCAGCTGGGTCCCGGAAGATGGTTCAGAGCTTGAAAGAGATAGTCAATAACTGCACTGAACAGGAGATCTATGCTATGCTTAAAGACTGTAACATGGACCCTAATGAAGCCGTTAATCGTCTCCTAGCTCAAGATGTCTCAGAGCCTAGTAAGGAGCCAACGGATTCTAGGTCTCGCGGTGCTAATATTTCAAATCATGGTGGTAGAGGTGGTGATCGTTATGCTGCACGTGGCGGATCAAACCACTTTAGCACAAATGACTCTGGTTCTTTGCATGGTAGACCTGCGTACAGGAAGGAAAATGGAACACCTGCCTATGCAGGTTCTTCCTCTTCTGCAGCTGGCACAACTGGATATAACTTGAGTCGACGACCTCCATCCTACAGTGATCCAGTGGCAGCTGAAAATAAAATTATGTCAGTAGGTGATGACGGAATTTCTTCATCCTCACAGCCTTCCTCTGGATATCAGTCTGCATGGGGAGGGGTGCCAGGTCAAGTTTCAATGGCTGACATTGTTAAGAAGGGTAGGCCGCAAGCCAAGGCACCCCCAACTTATAGTGTCAATCATCATGATACAGGGGCACCTCCTGCAGCAGGATACAATTCGAATTCATCGCAGACTGAGCCCTTGGCTGATGAGTGGCCCTTGATTGAGCATCCTCCAGGTGTTAGCATGTCCTCCATTTTAGGGGCACCTGCAAATTCTGAGCTCTATGCTAATTCATCTAGCGTGCCCTTGGATAAAGCTAATCAGCATATAAAATCCCAACTAGATGAGGTTGAGACGGAAGATGATGGTTCTGTTGAGGCACTGAATACAAGCCATGATGGACCTACTTCTGTTTCCGGTAGAAATGTGCAAGAAGATAACTCGGCAACTGCATCTGCTTTTGATAATAGCTTGTATGAGGATATCAATTCCTACCATCCAGAGAGACATGACCTTGAGGATAATGAAGCTGAGCATGATGCTTCATCAGTGGCTGCAAACTTGCAGCAGCTTGACTTACAGAAGGATGATAGGGGAGCACCACCTGAAGACGAGAACCCTCCCCCTGTAGTCATTCCAAATCACCTACAACTTCATACTCCAGACTGCTTGCACTTGAGCTTTGGAAGTTTCCGATCCGGCCCTGAGCCTGATCTTTCGAGCTCCCATCCAGTGAAAAGTGACTTGGAAGAGACATCTGTAGCAGTAGATGTTTCTGCAGTCGGGCACTCAGATGCTAGAAATCCCGACTACTATGGAGACGAGCATCTCATAAATGCCTCTGATGGAAGTTTGGTTCATAGAACTGGGGCCAGTGCTGGAGATTATGATTCTCCTTCAGTGTCACAACCAGAGGTCTTAAAACAGGAAACCCCTGAAGCTGGTCAGGGCATTCAATATACTTTTCCTTCTGCACCTGGATTTGCATATGACAGCTCCCAACAATTGAATATGGCATTTTCTCACCCACAGAGCTCACAAGTACAAAACAATGCTCCCTTTTCAAGTGTGATGCAGGGATATACGAATTCGTTGCCTAGCACTTTATTGGCTTCAAATGCTCAGAATGTAAGGGAGGATCTTCCATACTCACCTTTCCCTGTGACACAATCTATGCCTACAAAATATAGCAATGTGCCTTCTTCCATCAGCGGTCCCACCATGTCCATGCCAGAGGCTTTGAGAGCAGGTGGTATCTCTTCTGCTCAACCTACTCAACAGACCCTACCTGGTGCTGGTGTCGCGACTGGACCTGCTCTTCCACAACAGCTGTTGCACCCTTACTCTCAACCTAGTCTTCCTTTGGGACATTTTTCCAACATGATTGGTTATCCTTTCTTGCCTCAGAGCTACACATATATGCCATCAGCATTCCAGCAAGCTTTTGCTGGTAACAGCACATACCATCAGTCGCTGGCTGCAGTTCTTCCACAGTATAAAAATAGTGTTTCTGTCAGCAGTTTGCCCCAGTCTGCTAATATTCCCTCTGGTTATGGGTTTGGTAGTTCAAGTAACATTCCTGGAGGAAACTTTCCTCTGAATCCACCGTCGGCACCTACCGGTACAACAATTGGTTATGATGATGTTATAAGCTCTCAGTACAAGGACAGTAACCATTTGATGTCACTTCAGCAGAATGACAACTCAGGCATGTGGGTTCACGGACCTGGTTCCCGAGCAATGCCTGCTGTTCCAGCCAGCAATTATTACAGCTTCCAGGGGCAGAATCAACAGCATGCTGGTTTCCGGCAAACCCAGCAACCGTCGCAGCAATTTGCTGGAGCTCTAAACTACCCGAACTTCTATCATTCTCAGACAGGGATGTCCCTCGATCATCAACAGCAAAGTTCAAGGGACGCATCCCTGGGTGGCTCACAGGGACAACCTTCTAAGCAGTCTCAACAATTATGGCAAAACAGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

823

Amino Acids

87.28

Weight (kDa)

5.05

Isoelectric Point (pI)

62.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 18 - 64 2.6e-22 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2135
AarI CACCTGC 3 cut(s) 373, 760, 1724
AasI GACNNNNNNGTC 1 cut(s) 2368
Acc36I ACCTGC 6 cut(s) 346, 365, 373, 760, 1724, 1807
AccB1I GGYRCC 5 cut(s) 541, 598, 637, 748, 2098
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 2 cut(s) 334, 421
AccII CGCG 2 cut(s) 231, 1790
AciI CCGC 7 cut(s) 6, 15, 18, 231, 288, 587, 1698
AclWI GGATC 4 cut(s) 298, 437, 1176, 1632
AcsI RAATTY 4 cut(s) 489, 664, 757, 1573
AcuI CTGAAG 4 cut(s) 1113, 1368, 1443, 2159
AcvI CACGTG 1 cut(s) 284
AfaI GTAC 4 cut(s) 344, 1530, 2110, 2147
AfiI CCNNNNNNNGG 6 cut(s) 73, 515, 650, 744, 1427, 1781
AgeI ACCGGT 1 cut(s) 2105
AgsI TTSAA 9 cut(s) 88, 246, 558, 1442, 1495, 1551, 1602, 2055, 2397
AjnI CCWGG 8 cut(s) 544, 718, 1462, 1774, 2065, 2212, 2262, 2409
AjuI GAANNNNNNNTTGG 2 cut(s) 1214, 1246
AloI GAACNNNNNNTCC 2 cut(s) 1326, 1358
Alw21I GWGCWC 5 cut(s) 768, 1087, 1211, 1523, 2332
Alw26I GTCTC 8 cut(s) 179, 196, 231, 833, 985, 1232, 1299, 2448
AlwI GGATC 4 cut(s) 298, 437, 1176, 1632
AlwNI CAGNNNCTG 2 cut(s) 1040, 1960
Ama87I CYCGRG 1 cut(s) 2220
AoxI GGCC 4 cut(s) 584, 698, 1186, 1356
ApeKI GCWGC 9 cut(s) 278, 392, 452, 647, 1040, 1051, 1054, 1964, 2314
ApoI RAATTY 4 cut(s) 489, 664, 757, 1573
AsiGI ACCGGT 1 cut(s) 2105
Asp700I GAANNNNTTC 1 cut(s) 493
AspS9I GGNCC 9 cut(s) 63, 151, 699, 887, 1187, 1356, 1700, 1796, 2210
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 5 cut(s) 278, 491, 1121, 1502, 1629
AsuII TTCGAA 1 cut(s) 662
AvaI CYCGRG 1 cut(s) 2220
AvaII GGWCC 6 cut(s) 63, 151, 887, 1700, 1796, 2210
BaeGI GKGCMC 4 cut(s) 640, 751, 792, 1275
BanI GGYRCC 5 cut(s) 541, 598, 637, 748, 2098
BanII GRGCYC 5 cut(s) 684, 768, 1211, 1523, 2332
BbrPI CACGTG 1 cut(s) 284
BbsI GAAGAC 2 cut(s) 1101, 1835
Bbv12I GWGCWC 5 cut(s) 768, 1087, 1211, 1523, 2332
BbvI GCAGC 9 cut(s) 265, 404, 464, 659, 1027, 1063, 1066, 1951, 2326
BceAI ACGGC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 1561, 1595
BciT130I CCWGG 8 cut(s) 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
BciVI GTATCC 1 cut(s) 647
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 8 cut(s) 179, 196, 231, 833, 985, 1232, 1299, 2448
BfaI CTAG 8 cut(s) 179, 201, 222, 783, 827, 1284, 1584, 1839
BfmI CTRYAG 7 cut(s) 390, 436, 645, 1113, 1245, 1263, 1965
BfuAI ACCTGC 6 cut(s) 346, 365, 373, 760, 1724, 1807
BfuI GTATCC 1 cut(s) 647
BglII AGATCT 1 cut(s) 120
BlpI GCTNAGC 1 cut(s) 1017
Bme1390I CCNGG 9 cut(s) 67, 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
Bme18I GGWCC 6 cut(s) 63, 151, 887, 1700, 1796, 2210
BmeT110I CYCGRG 1 cut(s) 2220
BmgT120I GGNCC 9 cut(s) 63, 151, 699, 887, 1187, 1356, 1700, 1796, 2210
BmrFI CCNGG 9 cut(s) 67, 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
BmrI ACTGGG 2 cut(s) 1362, 2008
BmsI GCATC 7 cut(s) 721, 947, 1015, 1270, 1321, 1548, 2414
BmuI ACTGGG 2 cut(s) 1362, 2008
BpiI GAAGAC 2 cut(s) 1101, 1835
BpmI CTGGAG 5 cut(s) 702, 1131, 1386, 2088, 2346
Bpu10I CCTNAGC 1 cut(s) 1191
Bpu1102I GCTNAGC 1 cut(s) 1017
Bpu14I TTCGAA 1 cut(s) 662
BpuEI CTTGAG 4 cut(s) 168, 436, 1022, 1183
BpuMI CCSGG 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 284
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 6 cut(s) 594, 684, 792, 2263, 2409, 2410
BsaWI WCCGGW 2 cut(s) 902, 2105
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 515, 650, 744, 1427, 1781
Bse118I RCCGGY 1 cut(s) 2105
Bse1I ACTGG 8 cut(s) 43, 409, 446, 1217, 1357, 1359, 1798, 2014
Bse3DI GCAATG 3 cut(s) 28, 1684, 2232
BseBI CCWGG 8 cut(s) 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
BseDI CCNNGG 6 cut(s) 594, 684, 792, 2263, 2409, 2410
BseGI GGATG 8 cut(s) 431, 497, 712, 982, 1078, 1213, 2373, 2405
BseLI CCNNNNNNNGG 6 cut(s) 73, 515, 650, 744, 1427, 1781
BseMI GCAATG 3 cut(s) 28, 1684, 2232
BseNI ACTGG 8 cut(s) 43, 409, 446, 1217, 1357, 1359, 1798, 2014
BseSI GKGCMC 4 cut(s) 640, 751, 792, 1275
BseXI GCAGC 9 cut(s) 265, 404, 464, 659, 1027, 1063, 1066, 1951, 2326
BseYI CCCAGC 2 cut(s) 59, 2301
BsgI GTGCAG 3 cut(s) 91, 264, 1443
Bsh1236I CGCG 2 cut(s) 231, 1790
BshFI GGCC 4 cut(s) 586, 700, 1188, 1358
BshNI GGYRCC 5 cut(s) 541, 598, 637, 748, 2098
BshTI ACCGGT 1 cut(s) 2105
BsiHKAI GWGCWC 5 cut(s) 768, 1087, 1211, 1523, 2332
BsiHKCI CYCGRG 1 cut(s) 2220
BsiSI CCGG 5 cut(s) 67, 903, 1185, 2106, 2293
BslFI GGGAC 6 cut(s) 49, 1686, 1866, 2356, 2414, 2439
BslI CCNNNNNNNGG 6 cut(s) 73, 515, 650, 744, 1427, 1781
BsmAI GTCTC 8 cut(s) 179, 196, 231, 833, 985, 1232, 1299, 2448
BsmBI CGTCTC 3 cut(s) 179, 833, 1299
BsmFI GGGAC 6 cut(s) 49, 1686, 1866, 2356, 2414, 2439
BsmI GAATGC 2 cut(s) 1437, 1916
BsnI GGCC 4 cut(s) 586, 700, 1188, 1358
Bso31I GGTCTC 1 cut(s) 231
BsoBI CYCGRG 1 cut(s) 2220
Bsp119I TTCGAA 1 cut(s) 662
Bsp143I GATC 8 cut(s) 120, 268, 290, 442, 1181, 1198, 1624, 2377
Bsp1720I GCTNAGC 1 cut(s) 1017
Bsp68I TCGCGA 1 cut(s) 1790
BspACI CCGC 7 cut(s) 6, 15, 18, 231, 288, 587, 1698
BspANI GGCC 4 cut(s) 586, 700, 1188, 1358
BspFNI CGCG 2 cut(s) 231, 1790
BspHI TCATGA 1 cut(s) 625
BspMAI CTGCAG 4 cut(s) 394, 649, 1267, 1969
BspMI ACCTGC 6 cut(s) 346, 365, 373, 760, 1724, 1807
BspPI GGATC 4 cut(s) 298, 437, 1176, 1632
BspQI GCTCTTC 1 cut(s) 1809
BspT104I TTCGAA 1 cut(s) 662
BspT107I GGYRCC 5 cut(s) 541, 598, 637, 748, 2098
BspTNI GGTCTC 1 cut(s) 231
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 3 cut(s) 28, 1684, 2232
BsrFI RCCGGY 1 cut(s) 2105
BsrI ACTGG 8 cut(s) 43, 409, 446, 1217, 1357, 1359, 1798, 2014
BssAI RCCGGY 1 cut(s) 2105
BssECI CCNNGG 6 cut(s) 594, 684, 792, 2263, 2409, 2410
BssMI GATC 8 cut(s) 120, 268, 290, 442, 1181, 1198, 1624, 2377
BssT1I CCWWGG 3 cut(s) 594, 684, 792
Bst2UI CCWGG 8 cut(s) 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
Bst4CI ACNGT 6 cut(s) 143, 440, 1980, 2094, 2156, 2310
Bst6I CTCTTC 4 cut(s) 391, 1230, 1750, 1809
BstAPI GCANNNNNTGC 2 cut(s) 644, 2231
BstBAI YACGTR 1 cut(s) 284
BstBI TTCGAA 1 cut(s) 662
BstC8I GCNNGC 9 cut(s) 397, 591, 788, 1157, 1926, 1962, 2232, 2245, 2283
BstENI CCTNNNNNAGG 1 cut(s) 648
BstF5I GGATG 8 cut(s) 431, 497, 712, 982, 1078, 1213, 2373, 2405
BstFNI CGCG 2 cut(s) 231, 1790
BstKTI GATC 8 cut(s) 123, 271, 293, 445, 1184, 1201, 1627, 2380
BstMAI GTCTC 8 cut(s) 179, 196, 231, 833, 985, 1232, 1299, 2448
BstMBI GATC 8 cut(s) 120, 268, 290, 442, 1181, 1198, 1624, 2377
BstNI CCWGG 8 cut(s) 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
BstNSI RCATGY 3 cut(s) 733, 2200, 2285
BstSCI CCNGG 9 cut(s) 65, 544, 718, 1462, 1774, 2065, 2212, 2262, 2409
BstSFI CTRYAG 7 cut(s) 390, 436, 645, 1113, 1245, 1263, 1965
BstSLI GKGCMC 4 cut(s) 640, 751, 792, 1275
BstUI CGCG 2 cut(s) 231, 1790
BstV1I GCAGC 9 cut(s) 265, 404, 464, 659, 1027, 1063, 1066, 1951, 2326
BstV2I GAAGAC 2 cut(s) 1101, 1835
BstX2I RGATCY 2 cut(s) 120, 1624
BstYI RGATCY 2 cut(s) 120, 1624
BsuI GTATCC 1 cut(s) 647
BsuRI GGCC 4 cut(s) 586, 700, 1188, 1358
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 8 cut(s) 431, 497, 712, 982, 1078, 1213, 2373, 2405
BtsIMutI CAGTG 8 cut(s) 108, 445, 453, 866, 1041, 1224, 1366, 1392
BtuMI TCGCGA 1 cut(s) 1790
BveI ACCTGC 6 cut(s) 346, 365, 373, 760, 1724, 1807
Cac8I GCNNGC 9 cut(s) 397, 591, 788, 1157, 1926, 1962, 2232, 2245, 2283
CaiI CAGNNNCTG 2 cut(s) 1040, 1960
CciI TCATGA 1 cut(s) 625
Cfr10I RCCGGY 1 cut(s) 2105
Cfr13I GGNCC 9 cut(s) 63, 151, 699, 887, 1187, 1356, 1700, 1796, 2210
CseI GACGC 1 cut(s) 2411
CsiI ACCWGGT 2 cut(s) 1774, 2212
Csp6I GTAC 4 cut(s) 343, 1529, 2109, 2146
CspAI ACCGGT 1 cut(s) 2105
CviQI GTAC 4 cut(s) 343, 1529, 2109, 2146
DpnI GATC 8 cut(s) 122, 270, 292, 444, 1183, 1200, 1626, 2379
DpnII GATC 8 cut(s) 120, 268, 290, 442, 1181, 1198, 1624, 2377
DrdI GACNNNNNNGTC 1 cut(s) 2368
DseDI GACNNNNNNGTC 1 cut(s) 2368
Eam1104I CTCTTC 4 cut(s) 391, 1230, 1750, 1809
EarI CTCTTC 4 cut(s) 391, 1230, 1750, 1809
EciI GGCGGA 1 cut(s) 303
Ecl136II GAGCTC 4 cut(s) 766, 1209, 1521, 2330
Eco130I CCWWGG 3 cut(s) 594, 684, 792
Eco24I GRGCYC 5 cut(s) 684, 768, 1211, 1523, 2332
Eco31I GGTCTC 1 cut(s) 231
Eco32I GATATC 2 cut(s) 521, 970
Eco47I GGWCC 6 cut(s) 63, 151, 887, 1700, 1796, 2210
Eco53kI GAGCTC 4 cut(s) 766, 1209, 1521, 2330
Eco57I CTGAAG 4 cut(s) 1113, 1368, 1443, 2159
Eco72I CACGTG 1 cut(s) 284
Eco88I CYCGRG 1 cut(s) 2220
EcoICRI GAGCTC 4 cut(s) 766, 1209, 1521, 2330
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 63
EcoRI GAATTC 2 cut(s) 664, 1573
EcoRII CCWGG 8 cut(s) 544, 718, 1462, 1774, 2065, 2212, 2262, 2409
EcoRV GATATC 2 cut(s) 521, 970
EcoT14I CCWWGG 3 cut(s) 594, 684, 792
EcoT38I GRGCYC 5 cut(s) 684, 768, 1211, 1523, 2332
ErhI CCWWGG 3 cut(s) 594, 684, 792
Esp3I CGTCTC 3 cut(s) 179, 833, 1299
FaqI GGGAC 6 cut(s) 49, 1686, 1866, 2356, 2414, 2439
FauNDI CATATG 1 cut(s) 1474
FblI GTMKAC 2 cut(s) 334, 421
FokI GGATG 8 cut(s) 418, 484, 699, 969, 1085, 1200, 2380, 2392
FriOI GRGCYC 5 cut(s) 684, 768, 1211, 1523, 2332
FspBI CTAG 8 cut(s) 179, 201, 222, 783, 827, 1284, 1584, 1839
GsaI CCCAGC 2 cut(s) 63, 2305
GsuI CTGGAG 5 cut(s) 702, 1131, 1386, 2088, 2346
HaeIII GGCC 4 cut(s) 586, 700, 1188, 1358
HapII CCGG 5 cut(s) 67, 903, 1185, 2106, 2293
HgaI GACGC 1 cut(s) 2411
HincII GTYRAC 1 cut(s) 422
HindII GTYRAC 1 cut(s) 422
HindIII AAGCTT 1 cut(s) 1926
HinfI GANTC 6 cut(s) 218, 314, 418, 1376, 2086, 2272
HpaII CCGG 5 cut(s) 67, 903, 1185, 2106, 2293
HphI GGTGA 5 cut(s) 278, 491, 1121, 1502, 1629
Hpy166II GTNNAC 3 cut(s) 335, 422, 2206
Hpy188III TCNNGA 9 cut(s) 48, 185, 516, 626, 986, 1148, 1292, 1789, 2220
Hpy8I GTNNAC 3 cut(s) 335, 422, 2206
Hpy99I CGWCG 3 cut(s) 426, 2098, 2314
HpyAV CCTTC 9 cut(s) 22, 343, 519, 571, 1063, 1392, 1464, 1695, 2442
HpyCH4III ACNGT 6 cut(s) 143, 440, 1980, 2094, 2156, 2310
HpyCH4IV ACGT 1 cut(s) 283
HpySE526I ACGT 1 cut(s) 283
KflI GGGWCCC 1 cut(s) 63
Kzo9I GATC 8 cut(s) 120, 268, 290, 442, 1181, 1198, 1624, 2377
LguI GCTCTTC 1 cut(s) 1809
LmnI GCTCC 6 cut(s) 208, 1082, 1214, 1487, 1546, 2327
Lsp1109I GCAGC 9 cut(s) 265, 404, 464, 659, 1027, 1063, 1066, 1951, 2326
LweI GCATC 7 cut(s) 721, 947, 1015, 1270, 1321, 1548, 2414
MabI ACCWGGT 2 cut(s) 1774, 2212
MaeI CTAG 8 cut(s) 179, 201, 222, 783, 827, 1284, 1584, 1839
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 8 cut(s) 143, 1226, 1389, 1648, 1937, 2057, 2156, 2169
MalI GATC 8 cut(s) 122, 270, 292, 444, 1183, 1200, 1626, 2379
MbiI CCGCTC 1 cut(s) 6
MboI GATC 8 cut(s) 120, 268, 290, 442, 1181, 1198, 1624, 2377
MfeI CAATTG 2 cut(s) 1490, 2115
MflI RGATCY 2 cut(s) 120, 1624
MlyI GAGTC 2 cut(s) 308, 427
MmeI TCCRAC 1 cut(s) 1887
MroXI GAANNNNTTC 1 cut(s) 493
MseI TTAA 4 cut(s) 135, 168, 573, 1406
MslI CAYNNNNRTG 3 cut(s) 1278, 1712, 1904
MspA1I CMGCKG 5 cut(s) 59, 395, 455, 1698, 1816
MspI CCGG 5 cut(s) 67, 903, 1185, 2106, 2293
MspR9I CCNGG 9 cut(s) 67, 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
MunI CAATTG 2 cut(s) 1490, 2115
Mva1269I GAATGC 2 cut(s) 1437, 1916
MvaI CCWGG 8 cut(s) 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
MvnI CGCG 2 cut(s) 231, 1790
NciI CCSGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 1474
NdeII GATC 8 cut(s) 120, 268, 290, 442, 1181, 1198, 1624, 2377
NmeAIII GCCGAG 1 cut(s) 908
NmuCI GTSAC 4 cut(s) 1226, 1389, 1648, 2169
NruI TCGCGA 1 cut(s) 1790
NspI RCATGY 3 cut(s) 733, 2200, 2285
NspV TTCGAA 1 cut(s) 662
PaeI GCATGC 1 cut(s) 2285
PagI TCATGA 1 cut(s) 625
PaqCI CACCTGC 3 cut(s) 373, 760, 1724
PasI CCCWGGG 1 cut(s) 2410
PciSI GCTCTTC 1 cut(s) 1809
PctI GAATGC 2 cut(s) 1437, 1916
PdmI GAANNNNTTC 1 cut(s) 493
PfeI GAWTC 4 cut(s) 218, 1376, 2086, 2272
PfoI TCCNGGA 2 cut(s) 65, 2065
PinAI ACCGGT 1 cut(s) 2105
PleI GAGTC 2 cut(s) 308, 426
PmaCI CACGTG 1 cut(s) 284
PmlI CACGTG 1 cut(s) 284
PpsI GAGTC 2 cut(s) 308, 426
Ppu21I YACGTR 1 cut(s) 284
PpuMI RGGWCCY 1 cut(s) 63
PsiI TTATAA 1 cut(s) 2135
Psp124BI GAGCTC 4 cut(s) 768, 1211, 1523, 2332
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 8 cut(s) 544, 718, 1462, 1774, 2065, 2212, 2262, 2409
PspCI CACGTG 1 cut(s) 284
PspFI CCCAGC 2 cut(s) 59, 2301
PspGI CCWGG 8 cut(s) 544, 718, 1462, 1774, 2065, 2212, 2262, 2409
PspPI GGNCC 9 cut(s) 63, 151, 699, 887, 1187, 1356, 1700, 1796, 2210
PspPPI RGGWCCY 1 cut(s) 63
PstI CTGCAG 4 cut(s) 394, 649, 1267, 1969
PstNI CAGNNNCTG 2 cut(s) 1040, 1960
PsuI RGATCY 2 cut(s) 120, 1624
PvuII CAGCTG 4 cut(s) 59, 395, 455, 1816
RruI TCGCGA 1 cut(s) 1790
RsaI GTAC 4 cut(s) 344, 1530, 2110, 2147
RsaNI GTAC 4 cut(s) 343, 1529, 2109, 2146
RseI CAYNNNNRTG 3 cut(s) 1278, 1712, 1904
SacI GAGCTC 4 cut(s) 768, 1211, 1523, 2332
SalI GTCGAC 1 cut(s) 420
SapI GCTCTTC 1 cut(s) 1809
SaqAI TTAA 4 cut(s) 135, 168, 573, 1406
Sau3AI GATC 8 cut(s) 120, 268, 290, 442, 1181, 1198, 1624, 2377
Sau96I GGNCC 9 cut(s) 63, 151, 699, 887, 1187, 1356, 1700, 1796, 2210
SchI GAGTC 2 cut(s) 308, 427
ScrFI CCNGG 9 cut(s) 67, 546, 720, 1464, 1776, 2067, 2214, 2264, 2411
SexAI ACCWGGT 2 cut(s) 1774, 2212
SfaNI GCATC 7 cut(s) 721, 947, 1015, 1270, 1321, 1548, 2414
SfcI CTRYAG 7 cut(s) 390, 436, 645, 1113, 1245, 1263, 1965
SfuI TTCGAA 1 cut(s) 662
SinI GGWCC 6 cut(s) 63, 151, 887, 1700, 1796, 2210
SmiMI CAYNNNNRTG 3 cut(s) 1278, 1712, 1904
SmlI CTYRAG 4 cut(s) 183, 415, 1001, 1162
SmoI CTYRAG 4 cut(s) 183, 415, 1001, 1162
SphI GCATGC 1 cut(s) 2285
SsiI CCGC 7 cut(s) 6, 15, 18, 231, 288, 587, 1698
SspI AATATT 2 cut(s) 241, 2026
SspMI CTAG 8 cut(s) 179, 201, 222, 783, 827, 1284, 1584, 1839
SstI GAGCTC 4 cut(s) 768, 1211, 1523, 2332
StyD4I CCNGG 9 cut(s) 65, 544, 718, 1462, 1774, 2065, 2212, 2262, 2409
StyI CCWWGG 3 cut(s) 594, 684, 792
TaaI ACNGT 6 cut(s) 143, 440, 1980, 2094, 2156, 2310
TaiI ACGT 1 cut(s) 286
TaqI TCGA 4 cut(s) 421, 662, 1205, 2376
TatI WGTACW 2 cut(s) 1528, 2145
TauI GCSGC 3 cut(s) 18, 21, 589
TfiI GAWTC 4 cut(s) 218, 1376, 2086, 2272
Tru1I TTAA 4 cut(s) 135, 168, 573, 1406
Tru9I TTAA 4 cut(s) 135, 168, 573, 1406
TscAI CASTG 8 cut(s) 115, 445, 453, 873, 1041, 1224, 1366, 1392
TseFI GTSAC 4 cut(s) 1226, 1389, 1648, 2169
TseI GCWGC 9 cut(s) 278, 392, 452, 647, 1040, 1051, 1054, 1964, 2314
Tsp45I GTSAC 4 cut(s) 1226, 1389, 1648, 2169
TspDTI ATGAA 8 cut(s) 174, 486, 657, 768, 1020, 1026, 1130, 1334
TspGWI ACGGA 4 cut(s) 230, 501, 857, 2223
TspRI CASTG 8 cut(s) 115, 445, 453, 873, 1041, 1224, 1366, 1392
VpaK11BI GGWCC 6 cut(s) 63, 151, 887, 1700, 1796, 2210
XagI CCTNNNNNAGG 1 cut(s) 648
XapI RAATTY 4 cut(s) 489, 664, 757, 1573
XceI RCATGY 3 cut(s) 733, 2200, 2285
XmiI GTMKAC 2 cut(s) 334, 421
XmnI GAANNNNTTC 1 cut(s) 493
XspI CTAG 8 cut(s) 179, 201, 222, 783, 827, 1284, 1584, 1839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.