Rroxscaffold_1G00063560

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006562
Physical Location & Seq
Reverse (-)
85405567 .. 85411510
5944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063560.1

Sequence Viewer

Length: 2502 bp
ATGAGCGGGAGAGGCGGCGGCAATGGGAAGGGCAATACTGGAATATTGGGAATACCAGCTGGGTCCCGGAAGATGGTTCAGAGCTTGAAAGAGATAGTCAATAACTGCACTGAGCAGGAGATCTATGCTATGCTTAAAGACTGTAACATGGACCCTAATGAAGCTGTTAATCGTCTCCTAGCACAAGATCCTTTCCATGAAGTGAAGAGCAAACGAGAGAAGAAAAAGGAGAGTAAGGAGCCAACGGATTCTAGGTCTCGCGGTGCTAATATTTCAAATCATGGTGGTAGAGGTGGTGATCGTTATGCTGCACGTGGCGGATCAAACCACTTTAGCACAAATGAGTCTGGTTCTTTGCATGGTAGACCTGCGTACAGAAAGGAAAATGGAACACCTGCCTATGCAGGTTCTTCCTCTTCTGCAGCTGGCACAACTGGATATAACTTGAGCCGACGACCTCCATCCTACAGTGATCCAGTGGCAGCTGAAAATAAAATTATGTCAGTAGGTGATGACGGAATATCTTCGTCTTCACAGCCTTCCTCTGGATATCAGTCTGCATGGGGAGGGGTGCCAGGTCAAGTTTCAATGGCTGACATTGTTAAGAAGGGTAGGCCGCAAGCCAAGGCACCCCCAACTTATAGTGTCAATCATCATGACACAGGGGCACCTCCTGTAGCAGGATACAATTCGAATTCATCGCAGACTGAGCCCTTGGCTGATGAGTGGCCCTTGATTGAGCATCCTCCAGGTGTTAGCATGTCCTCCATTTTAGGGGCACCTGCAAATTCTGAGCTCTATGCTAATTCATCTAGCGTGCCCTTGGATAAAGCTAATCAGCATATAAAATCCCAACTAGATGAGGTTGAGACGGAAGATGATGGTTCTGTTGAGGCACTGAATACAAGCCATGATGGACCTACTTCTGTTTCCGGTAGAAATGTGCAAGAAGATAACTCGGCAACTGCATCTGCTTTTGATAATAGCTTGTATGAGGATATCAATTCTTACCATCCAGAGAGACATGACCTTGAGGATAATGAAGCTGAGCATGATGCTTCATCAGTGGCTGCAAACTTGCAGCAGCTTGACTTACAGAAGGATGATAGGGGAGCACCACCTGAAGACGAGAACCCTCCCCCTGTAGTCATTCCAAATCACCTACAACTTCATACTCCAGACTGCTTGCACTTGAGCTTTGGAAGTTTCCGATCTGGTCCTGAGCCTGATCTTTCGAGCTCCCATCCAGTGAAAAGTGACTTGGAAGAGACATCTGTAGCAGTAGATGTTTCTGCAGTCGGGCACTCAGATGCTAGAAATCCTGACTACTATGGAGATGAGCATCTCATAAATACCTCTGATGGAAGTTTGGTTCATAGAACTGGGGCCAGTGCTGGAGATTATGATTCTCCTTCAGTGTCACAACCAGAGGTCTTAAAACAGGAAACCTCTGAAGCTGGTCAGGGCATTCAATATACTTTTCCTTCTGCACCTGGATTTGCATATGACAGCTCCCAACAATTGAATATGGCATTTTCTCACCCACAGAGCTCACAAGTACAAAACAATGCTCCCTTTTCAAGTGTGATGCAGGGATATACAAATTCGTTGCCTAGCACTCTATTGGCTTCAAATGCTCAGAATGTAAGGGAGGATCTTCCATACTCACCTTTCCCTGTGACACAATCTATGCCTACAAAATATAGCAATGTGCCTTCTTCCATCAGTGGTCCCACCATGTCCATGCCAGAGGCTTTGAGAGCAGGTGGTATCTCTTCTGCTCAACCTACTCAACAGACCCTACCTGGTGCTGGTGTCGCGACTGGACCTGCTCTTCCACAACAGCTGTTGCACCCTTACTCTCAACCTAGTCTTCCTTTGGGACATTTTTCCAACATGATTGGTTATCCTTTCTTGCCTCAGAGCTACACATATATGCCATCAGCATTCCAGCAAGCTTTTGCTGGTAACAGCACATACCATCAGTCGCTGGCTGCAGTTCTCCCACAGTATAAAAATAGTGTTTCTGTCAGCAGTTTGCCCCAGTCTGCTAATATTCCCTCTGGTTATGGGTTTGGTAGTTCAAGTAACATTCCTGGAGGAAACTTTCCTCTGAATCCACCGTCGGCACCTACCGGTACAACAATTGGTTATGATGATGTTATAAGCTCTCAGTACAAGGACAGTAACCATTTGATGTCACTTCAGCAGAATGACAACTCAGGCATGTGGGTTCACGGACCTGGTTCCCGAGCAATGCCTGCTGTTCCAGCCAGCAATTATTACAGCTTCCAGGGGCAGAATCAACAGCATGCTGGTTTCCGGCAAACCCAGCAACCGTCGCAGCAATTTGCTGGAGCTCTAAACTACCCGAACTTCTATCATTCTCAGACAGGGATGTCCCTCGATCATCAACAGCAAAGTTCAAGGGACGCATCCCTGGGTGGCTCACAGGGACAACCTTCTAAGCAGTCTCAACAATTATGGCAAAACAGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

833

Amino Acids

88.71

Weight (kDa)

5.26

Isoelectric Point (pI)

62.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 18 - 77 9.3e-34 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2165
AarI CACCTGC 3 cut(s) 403, 790, 1754
AasI GACNNNNNNGTC 1 cut(s) 2398
Acc36I ACCTGC 6 cut(s) 376, 395, 403, 790, 1754, 1837
AccB1I GGYRCC 5 cut(s) 571, 628, 667, 778, 2128
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 364
AccII CGCG 2 cut(s) 261, 1820
AciI CCGC 6 cut(s) 6, 15, 18, 261, 318, 617
AclWI GGATC 4 cut(s) 182, 328, 467, 1662
AcsI RAATTY 3 cut(s) 694, 787, 1603
AcuI CTGAAG 4 cut(s) 1143, 1398, 1473, 2189
AcvI CACGTG 1 cut(s) 314
AfaI GTAC 4 cut(s) 374, 1560, 2140, 2177
AfiI CCNNNNNNNGG 5 cut(s) 73, 545, 680, 774, 1811
AgeI ACCGGT 1 cut(s) 2135
AgsI TTSAA 9 cut(s) 88, 276, 588, 1472, 1525, 1581, 1632, 2085, 2427
AjnI CCWGG 8 cut(s) 574, 748, 1492, 1804, 2095, 2242, 2292, 2439
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
AloI GAACNNNNNNTCC 2 cut(s) 1356, 1388
Alw21I GWGCWC 5 cut(s) 798, 1117, 1241, 1553, 2362
Alw26I GTCTC 6 cut(s) 179, 261, 863, 1015, 1262, 2478
AlwI GGATC 4 cut(s) 182, 328, 467, 1662
AlwNI CAGNNNCTG 2 cut(s) 1070, 1990
Ama87I CYCGRG 1 cut(s) 2250
AoxI GGCC 3 cut(s) 614, 728, 1386
ApeKI GCWGC 8 cut(s) 308, 422, 482, 1070, 1081, 1084, 1994, 2344
ApoI RAATTY 3 cut(s) 694, 787, 1603
AsiGI ACCGGT 1 cut(s) 2135
Asp700I GAANNNNTTC 1 cut(s) 523
AspS9I GGNCC 9 cut(s) 63, 151, 729, 917, 1217, 1386, 1730, 1826, 2240
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 5 cut(s) 308, 521, 1151, 1532, 1659
AsuII TTCGAA 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 2250
AvaII GGWCC 7 cut(s) 63, 151, 917, 1217, 1730, 1826, 2240
BaeGI GKGCMC 4 cut(s) 670, 781, 822, 1305
BanI GGYRCC 5 cut(s) 571, 628, 667, 778, 2128
BanII GRGCYC 5 cut(s) 714, 798, 1241, 1553, 2362
BbrPI CACGTG 1 cut(s) 314
BbsI GAAGAC 3 cut(s) 522, 1131, 1865
Bbv12I GWGCWC 5 cut(s) 798, 1117, 1241, 1553, 2362
BbvI GCAGC 8 cut(s) 295, 434, 494, 1057, 1093, 1096, 1981, 2356
BciT130I CCWGG 8 cut(s) 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
BciVI GTATCC 1 cut(s) 677
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 6 cut(s) 179, 261, 863, 1015, 1262, 2478
BfaI CTAG 7 cut(s) 179, 252, 813, 857, 1314, 1614, 1869
BfmI CTRYAG 7 cut(s) 420, 466, 675, 1143, 1275, 1293, 1995
BfuAI ACCTGC 6 cut(s) 376, 395, 403, 790, 1754, 1837
BfuI GTATCC 1 cut(s) 677
BglII AGATCT 1 cut(s) 120
BlpI GCTNAGC 1 cut(s) 1047
Bme1390I CCNGG 9 cut(s) 67, 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
Bme18I GGWCC 7 cut(s) 63, 151, 917, 1217, 1730, 1826, 2240
BmeT110I CYCGRG 1 cut(s) 2250
BmgT120I GGNCC 9 cut(s) 63, 151, 729, 917, 1217, 1386, 1730, 1826, 2240
BmrFI CCNGG 9 cut(s) 67, 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
BmrI ACTGGG 2 cut(s) 1392, 2038
BmsI GCATC 7 cut(s) 751, 977, 1045, 1300, 1351, 1578, 2444
BmuI ACTGGG 2 cut(s) 1392, 2038
BpiI GAAGAC 3 cut(s) 522, 1131, 1865
BpmI CTGGAG 5 cut(s) 732, 1161, 1416, 2118, 2376
Bpu10I CCTNAGC 1 cut(s) 1221
Bpu1102I GCTNAGC 1 cut(s) 1047
Bpu14I TTCGAA 1 cut(s) 692
BpuEI CTTGAG 3 cut(s) 466, 1052, 1213
BpuMI CCSGG 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 1341
BsaI GGTCTC 1 cut(s) 261
BsaJI CCNNGG 6 cut(s) 624, 714, 822, 2293, 2439, 2440
BsaWI WCCGGW 2 cut(s) 932, 2135
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 545, 680, 774, 1811
Bse118I RCCGGY 1 cut(s) 2135
Bse1I ACTGG 8 cut(s) 43, 439, 476, 1247, 1387, 1389, 1828, 2044
Bse3DI GCAATG 3 cut(s) 28, 1714, 2262
Bse8I GATNNNNATC 1 cut(s) 1341
BseBI CCWGG 8 cut(s) 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
BseDI CCNNGG 6 cut(s) 624, 714, 822, 2293, 2439, 2440
BseGI GGATG 7 cut(s) 461, 742, 1012, 1108, 1243, 2403, 2435
BseJI GATNNNNATC 1 cut(s) 1341
BseLI CCNNNNNNNGG 5 cut(s) 73, 545, 680, 774, 1811
BseMI GCAATG 3 cut(s) 28, 1714, 2262
BseNI ACTGG 8 cut(s) 43, 439, 476, 1247, 1387, 1389, 1828, 2044
BseSI GKGCMC 4 cut(s) 670, 781, 822, 1305
BseXI GCAGC 8 cut(s) 295, 434, 494, 1057, 1093, 1096, 1981, 2356
BseYI CCCAGC 2 cut(s) 59, 2331
BsgI GTGCAG 3 cut(s) 91, 294, 1473
Bsh1236I CGCG 2 cut(s) 261, 1820
BshFI GGCC 3 cut(s) 616, 730, 1388
BshNI GGYRCC 5 cut(s) 571, 628, 667, 778, 2128
BshTI ACCGGT 1 cut(s) 2135
BsiHKAI GWGCWC 5 cut(s) 798, 1117, 1241, 1553, 2362
BsiHKCI CYCGRG 1 cut(s) 2250
BsiSI CCGG 4 cut(s) 67, 933, 2136, 2323
BslFI GGGAC 6 cut(s) 49, 1716, 1896, 2386, 2444, 2469
BslI CCNNNNNNNGG 5 cut(s) 73, 545, 680, 774, 1811
BsmAI GTCTC 6 cut(s) 179, 261, 863, 1015, 1262, 2478
BsmBI CGTCTC 2 cut(s) 179, 863
BsmFI GGGAC 6 cut(s) 49, 1716, 1896, 2386, 2444, 2469
BsmI GAATGC 2 cut(s) 1467, 1946
BsnI GGCC 3 cut(s) 616, 730, 1388
Bso31I GGTCTC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 2250
Bsp119I TTCGAA 1 cut(s) 692
Bsp143I GATC 9 cut(s) 120, 187, 298, 320, 472, 1211, 1228, 1654, 2407
Bsp1720I GCTNAGC 1 cut(s) 1047
Bsp68I TCGCGA 1 cut(s) 1820
BspACI CCGC 6 cut(s) 6, 15, 18, 261, 318, 617
BspANI GGCC 3 cut(s) 616, 730, 1388
BspFNI CGCG 2 cut(s) 261, 1820
BspHI TCATGA 1 cut(s) 655
BspMAI CTGCAG 3 cut(s) 424, 1297, 1999
BspMI ACCTGC 6 cut(s) 376, 395, 403, 790, 1754, 1837
BspPI GGATC 4 cut(s) 182, 328, 467, 1662
BspQI GCTCTTC 2 cut(s) 200, 1839
BspT104I TTCGAA 1 cut(s) 692
BspT107I GGYRCC 5 cut(s) 571, 628, 667, 778, 2128
BspTNI GGTCTC 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 3 cut(s) 28, 1714, 2262
BsrFI RCCGGY 1 cut(s) 2135
BsrI ACTGG 8 cut(s) 43, 439, 476, 1247, 1387, 1389, 1828, 2044
BssAI RCCGGY 1 cut(s) 2135
BssECI CCNNGG 6 cut(s) 624, 714, 822, 2293, 2439, 2440
BssMI GATC 9 cut(s) 120, 187, 298, 320, 472, 1211, 1228, 1654, 2407
BssT1I CCWWGG 3 cut(s) 624, 714, 822
Bst2UI CCWGG 8 cut(s) 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
Bst4CI ACNGT 6 cut(s) 143, 470, 2010, 2124, 2186, 2340
Bst6I CTCTTC 5 cut(s) 200, 421, 1260, 1780, 1839
BstAPI GCANNNNNTGC 1 cut(s) 2261
BstBAI YACGTR 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 692
BstC8I GCNNGC 9 cut(s) 427, 621, 818, 1187, 1956, 1992, 2262, 2275, 2313
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 7 cut(s) 461, 742, 1012, 1108, 1243, 2403, 2435
BstFNI CGCG 2 cut(s) 261, 1820
BstKTI GATC 9 cut(s) 123, 190, 301, 323, 475, 1214, 1231, 1657, 2410
BstMAI GTCTC 6 cut(s) 179, 261, 863, 1015, 1262, 2478
BstMBI GATC 9 cut(s) 120, 187, 298, 320, 472, 1211, 1228, 1654, 2407
BstMWI GCNNNNNNNGC 9 cut(s) 12, 709, 1634, 1760, 1817, 2261, 2270, 2332, 2341
BstNI CCWGG 8 cut(s) 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
BstNSI RCATGY 3 cut(s) 763, 2230, 2315
BstSCI CCNGG 9 cut(s) 65, 574, 748, 1492, 1804, 2095, 2242, 2292, 2439
BstSFI CTRYAG 7 cut(s) 420, 466, 675, 1143, 1275, 1293, 1995
BstSLI GKGCMC 4 cut(s) 670, 781, 822, 1305
BstUI CGCG 2 cut(s) 261, 1820
BstV1I GCAGC 8 cut(s) 295, 434, 494, 1057, 1093, 1096, 1981, 2356
BstV2I GAAGAC 3 cut(s) 522, 1131, 1865
BstX2I RGATCY 3 cut(s) 120, 187, 1654
BstYI RGATCY 3 cut(s) 120, 187, 1654
BsuI GTATCC 1 cut(s) 677
BsuRI GGCC 3 cut(s) 616, 730, 1388
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 7 cut(s) 461, 742, 1012, 1108, 1243, 2403, 2435
BtsIMutI CAGTG 9 cut(s) 108, 475, 483, 896, 1071, 1254, 1396, 1422, 1732
BtuMI TCGCGA 1 cut(s) 1820
BveI ACCTGC 6 cut(s) 376, 395, 403, 790, 1754, 1837
Cac8I GCNNGC 9 cut(s) 427, 621, 818, 1187, 1956, 1992, 2262, 2275, 2313
CaiI CAGNNNCTG 2 cut(s) 1070, 1990
CciI TCATGA 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 2135
Cfr13I GGNCC 9 cut(s) 63, 151, 729, 917, 1217, 1386, 1730, 1826, 2240
CseI GACGC 1 cut(s) 2441
CsiI ACCWGGT 2 cut(s) 1804, 2242
Csp6I GTAC 4 cut(s) 373, 1559, 2139, 2176
CspAI ACCGGT 1 cut(s) 2135
CviQI GTAC 4 cut(s) 373, 1559, 2139, 2176
DpnI GATC 9 cut(s) 122, 189, 300, 322, 474, 1213, 1230, 1656, 2409
DpnII GATC 9 cut(s) 120, 187, 298, 320, 472, 1211, 1228, 1654, 2407
DrdI GACNNNNNNGTC 1 cut(s) 2398
DseDI GACNNNNNNGTC 1 cut(s) 2398
Eam1104I CTCTTC 5 cut(s) 200, 421, 1260, 1780, 1839
EarI CTCTTC 5 cut(s) 200, 421, 1260, 1780, 1839
EciI GGCGGA 1 cut(s) 333
Ecl136II GAGCTC 4 cut(s) 796, 1239, 1551, 2360
Eco130I CCWWGG 3 cut(s) 624, 714, 822
Eco24I GRGCYC 5 cut(s) 714, 798, 1241, 1553, 2362
Eco31I GGTCTC 1 cut(s) 261
Eco32I GATATC 2 cut(s) 551, 1000
Eco47I GGWCC 7 cut(s) 63, 151, 917, 1217, 1730, 1826, 2240
Eco53kI GAGCTC 4 cut(s) 796, 1239, 1551, 2360
Eco57I CTGAAG 4 cut(s) 1143, 1398, 1473, 2189
Eco72I CACGTG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 2250
EcoICRI GAGCTC 4 cut(s) 796, 1239, 1551, 2360
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 694
EcoRII CCWGG 8 cut(s) 574, 748, 1492, 1804, 2095, 2242, 2292, 2439
EcoRV GATATC 2 cut(s) 551, 1000
EcoT14I CCWWGG 3 cut(s) 624, 714, 822
EcoT38I GRGCYC 5 cut(s) 714, 798, 1241, 1553, 2362
ErhI CCWWGG 3 cut(s) 624, 714, 822
Esp3I CGTCTC 2 cut(s) 179, 863
FaqI GGGAC 6 cut(s) 49, 1716, 1896, 2386, 2444, 2469
FauNDI CATATG 1 cut(s) 1504
FblI GTMKAC 1 cut(s) 364
FokI GGATG 7 cut(s) 448, 729, 999, 1115, 1230, 2410, 2422
FriOI GRGCYC 5 cut(s) 714, 798, 1241, 1553, 2362
FspBI CTAG 7 cut(s) 179, 252, 813, 857, 1314, 1614, 1869
GsaI CCCAGC 2 cut(s) 63, 2335
GsuI CTGGAG 5 cut(s) 732, 1161, 1416, 2118, 2376
HaeIII GGCC 3 cut(s) 616, 730, 1388
HapII CCGG 4 cut(s) 67, 933, 2136, 2323
HgaI GACGC 1 cut(s) 2441
HindIII AAGCTT 1 cut(s) 1956
HinfI GANTC 5 cut(s) 248, 344, 1406, 2116, 2302
HpaII CCGG 4 cut(s) 67, 933, 2136, 2323
HphI GGTGA 5 cut(s) 308, 521, 1151, 1532, 1659
Hpy166II GTNNAC 2 cut(s) 365, 2236
Hpy188III TCNNGA 8 cut(s) 546, 656, 1016, 1178, 1220, 1322, 1819, 2250
Hpy8I GTNNAC 2 cut(s) 365, 2236
Hpy99I CGWCG 3 cut(s) 456, 2128, 2344
HpyAV CCTTC 8 cut(s) 22, 549, 601, 1093, 1422, 1494, 1725, 2472
HpyCH4III ACNGT 6 cut(s) 143, 470, 2010, 2124, 2186, 2340
HpyCH4IV ACGT 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 9 cut(s) 12, 709, 1634, 1760, 1817, 2261, 2270, 2332, 2341
HpySE526I ACGT 1 cut(s) 313
KflI GGGWCCC 1 cut(s) 63
Kzo9I GATC 9 cut(s) 120, 187, 298, 320, 472, 1211, 1228, 1654, 2407
LguI GCTCTTC 2 cut(s) 200, 1839
LmnI GCTCC 6 cut(s) 238, 1112, 1244, 1517, 1576, 2357
Lsp1109I GCAGC 8 cut(s) 295, 434, 494, 1057, 1093, 1096, 1981, 2356
LweI GCATC 7 cut(s) 751, 977, 1045, 1300, 1351, 1578, 2444
MabI ACCWGGT 2 cut(s) 1804, 2242
MaeI CTAG 7 cut(s) 179, 252, 813, 857, 1314, 1614, 1869
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 8 cut(s) 143, 1256, 1419, 1678, 1967, 2087, 2186, 2199
MalI GATC 9 cut(s) 122, 189, 300, 322, 474, 1213, 1230, 1656, 2409
MbiI CCGCTC 1 cut(s) 6
MboI GATC 9 cut(s) 120, 187, 298, 320, 472, 1211, 1228, 1654, 2407
MfeI CAATTG 2 cut(s) 1520, 2145
MflI RGATCY 3 cut(s) 120, 187, 1654
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 1917
MroXI GAANNNNTTC 1 cut(s) 523
MseI TTAA 4 cut(s) 135, 168, 603, 1436
MslI CAYNNNNRTG 3 cut(s) 1308, 1742, 1934
MspA1I CMGCKG 4 cut(s) 59, 425, 485, 1846
MspI CCGG 4 cut(s) 67, 933, 2136, 2323
MspR9I CCNGG 9 cut(s) 67, 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
MunI CAATTG 2 cut(s) 1520, 2145
Mva1269I GAATGC 2 cut(s) 1467, 1946
MvaI CCWGG 8 cut(s) 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
MvnI CGCG 2 cut(s) 261, 1820
MwoI GCNNNNNNNGC 9 cut(s) 12, 709, 1634, 1760, 1817, 2261, 2270, 2332, 2341
NciI CCSGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 1504
NdeII GATC 9 cut(s) 120, 187, 298, 320, 472, 1211, 1228, 1654, 2407
NmeAIII GCCGAG 1 cut(s) 938
NmuCI GTSAC 4 cut(s) 1256, 1419, 1678, 2199
NruI TCGCGA 1 cut(s) 1820
NspI RCATGY 3 cut(s) 763, 2230, 2315
NspV TTCGAA 1 cut(s) 692
PaeI GCATGC 1 cut(s) 2315
PagI TCATGA 1 cut(s) 655
PaqCI CACCTGC 3 cut(s) 403, 790, 1754
PasI CCCWGGG 1 cut(s) 2440
PciSI GCTCTTC 2 cut(s) 200, 1839
PctI GAATGC 2 cut(s) 1467, 1946
PdmI GAANNNNTTC 1 cut(s) 523
PfeI GAWTC 4 cut(s) 248, 1406, 2116, 2302
PfoI TCCNGGA 2 cut(s) 65, 2095
PinAI ACCGGT 1 cut(s) 2135
PleI GAGTC 1 cut(s) 352
PmaCI CACGTG 1 cut(s) 314
PmlI CACGTG 1 cut(s) 314
PpsI GAGTC 1 cut(s) 352
Ppu21I YACGTR 1 cut(s) 314
PpuMI RGGWCCY 1 cut(s) 63
PsiI TTATAA 1 cut(s) 2165
Psp124BI GAGCTC 4 cut(s) 798, 1241, 1553, 2362
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 8 cut(s) 574, 748, 1492, 1804, 2095, 2242, 2292, 2439
PspCI CACGTG 1 cut(s) 314
PspFI CCCAGC 2 cut(s) 59, 2331
PspGI CCWGG 8 cut(s) 574, 748, 1492, 1804, 2095, 2242, 2292, 2439
PspPI GGNCC 9 cut(s) 63, 151, 729, 917, 1217, 1386, 1730, 1826, 2240
PspPPI RGGWCCY 1 cut(s) 63
PstI CTGCAG 3 cut(s) 424, 1297, 1999
PstNI CAGNNNCTG 2 cut(s) 1070, 1990
PsuI RGATCY 3 cut(s) 120, 187, 1654
PvuII CAGCTG 4 cut(s) 59, 425, 485, 1846
RruI TCGCGA 1 cut(s) 1820
RsaI GTAC 4 cut(s) 374, 1560, 2140, 2177
RsaNI GTAC 4 cut(s) 373, 1559, 2139, 2176
RseI CAYNNNNRTG 3 cut(s) 1308, 1742, 1934
SacI GAGCTC 4 cut(s) 798, 1241, 1553, 2362
SapI GCTCTTC 2 cut(s) 200, 1839
SaqAI TTAA 4 cut(s) 135, 168, 603, 1436
Sau3AI GATC 9 cut(s) 120, 187, 298, 320, 472, 1211, 1228, 1654, 2407
Sau96I GGNCC 9 cut(s) 63, 151, 729, 917, 1217, 1386, 1730, 1826, 2240
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 9 cut(s) 67, 576, 750, 1494, 1806, 2097, 2244, 2294, 2441
SexAI ACCWGGT 2 cut(s) 1804, 2242
SfaNI GCATC 7 cut(s) 751, 977, 1045, 1300, 1351, 1578, 2444
SfcI CTRYAG 7 cut(s) 420, 466, 675, 1143, 1275, 1293, 1995
SfuI TTCGAA 1 cut(s) 692
SinI GGWCC 7 cut(s) 63, 151, 917, 1217, 1730, 1826, 2240
SmiMI CAYNNNNRTG 3 cut(s) 1308, 1742, 1934
SmlI CTYRAG 3 cut(s) 445, 1031, 1192
SmoI CTYRAG 3 cut(s) 445, 1031, 1192
SphI GCATGC 1 cut(s) 2315
SsiI CCGC 6 cut(s) 6, 15, 18, 261, 318, 617
SspI AATATT 3 cut(s) 45, 271, 2056
SspMI CTAG 7 cut(s) 179, 252, 813, 857, 1314, 1614, 1869
SstI GAGCTC 4 cut(s) 798, 1241, 1553, 2362
StyD4I CCNGG 9 cut(s) 65, 574, 748, 1492, 1804, 2095, 2242, 2292, 2439
StyI CCWWGG 3 cut(s) 624, 714, 822
TaaI ACNGT 6 cut(s) 143, 470, 2010, 2124, 2186, 2340
TaiI ACGT 1 cut(s) 316
TaqI TCGA 3 cut(s) 692, 1235, 2406
TatI WGTACW 2 cut(s) 1558, 2175
TauI GCSGC 3 cut(s) 18, 21, 619
TfiI GAWTC 4 cut(s) 248, 1406, 2116, 2302
Tru1I TTAA 4 cut(s) 135, 168, 603, 1436
Tru9I TTAA 4 cut(s) 135, 168, 603, 1436
TscAI CASTG 9 cut(s) 115, 475, 483, 903, 1071, 1254, 1396, 1422, 1732
TseFI GTSAC 4 cut(s) 1256, 1419, 1678, 2199
TseI GCWGC 8 cut(s) 308, 422, 482, 1070, 1081, 1084, 1994, 2344
Tsp45I GTSAC 4 cut(s) 1256, 1419, 1678, 2199
TspDTI ATGAA 8 cut(s) 174, 213, 687, 798, 1050, 1056, 1160, 1364
TspGWI ACGGA 4 cut(s) 260, 531, 887, 2253
TspRI CASTG 9 cut(s) 115, 475, 483, 903, 1071, 1254, 1396, 1422, 1732
VpaK11BI GGWCC 7 cut(s) 63, 151, 917, 1217, 1730, 1826, 2240
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 3 cut(s) 694, 787, 1603
XceI RCATGY 3 cut(s) 763, 2230, 2315
XmiI GTMKAC 1 cut(s) 364
XmnI GAANNNNTTC 1 cut(s) 523
XspI CTAG 7 cut(s) 179, 252, 813, 857, 1314, 1614, 1869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.