Rh5CG109300

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0006562
Physical Location & Seq
Forward (+)
8992941 .. 8999489
6549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG109300.1

Sequence Viewer

Length: 2508 bp
ATGAGCGGGAGAGGCGGCGGCAATGGGAAGGGCAATACTGGAATATCGGGAATACCAGCTGGGTCCCGGAAGATGGTTCAGAGCTTGAAAGAGATAGTCAATAACTGCACTGAGCAGGAGATCTATGCTATGCTTAAAGACTGTAACATGGACCCTAATGAAGCCGTTAATCGTCTCCTAGCTCAAGATCCTTTCCATGAAGTGAAGAGCAAACGAGAGAAGAAAAAGGAGAGTAAGGAGCCAACGGATTCTAGGTCTCGTGGTGCTAATATTTCAAATCATGGTGGTAGAGGTGGTGATCGTTATGCTGCACGTGGCGGATCAAACCACTTTAGCACAAATGAGTCTGGTTCTTTGCATGGTAGACCTGCGTACAGAAAGGAAAATGGAACACCTGCCTATGCAGGTTCTTCCTCTTCTGCAGCTGGCACAACTGGATATAACTTGAGCCGACGACCTCCATCCTACAGTGATCCAGTGGCAGCTGAAAATAAAATTATGTCAGTAGGTGATGACGGAATATCTTCATCCTCACAGCCTTCCTCTGGATATCAGTCTGCATGGGGAGGGGTGCCAGGTCAAGTTTCAATGGCTGACATTGTTAAGAAGGGTAGGCCGCAAGCCAAGGCACCCCCAACTTATAGTGTCAATCATCATGATACAGGGGCACCTCCTGCAGCAGGATACAATTCGAATTCATCGCAGACTGAGCCCTTGGCTGATGAGTGGCCCTTGATTGAGCATCCTCCAGGTGTTAGCATGTCCTCCATTTTAGGGGCACCTGCAAATTCTGAGCTCTATGCTAATTCATCTAGCGTGCCCTTGGATAAAGCTAATCAACATATAAAATCCCAACTAGATGAGGTTGAGACGGAAGATGATGGTTCTGTCGAGGTCGAGGCACTGAATACAAGCCATGATGGACCTACTTCTGTTTCCGGTAGAAATGTGCAAGAAGATAACTCGGCAACTGCATCTGCTTTTGATAATAGCTTGTATGAGGATATCAATTCCTACCATCCAGAGAGACATGACCTTGAGGATAATGAAGCTGAGCATGATGCTTCATCAATGGCTGCAAACTTGCAGCAGCTTGACTTACAGAAGGATGATAGGGGAGCACCACCTGAAGACGAGAACCCTCCCCCTGTAGTGATTCCAAATCACCTACAACTTCATACTCCAGACTGCTTGCACTTGAGCTTTGGAAGTTTCCGGTCCGGCCCTGAGCCTGATCTTTCGAGCTCCCATCCAGTGAAAAGTGACTTGGAAGAGACATCTGTAGCAGTAGATGTTTCTGCAGTCGGGCACTCAGATGCTAGAAATCCCGACTACTATGGAGATGAGCATCTCATAAATACCTCTGATGGAAGTTTGGTTCATAGAACTGGGGCCAGTGCTGGAGATTATGATTCTCCTTCAGTGTCACAACCAGAGGTCTTAAAACAGGAAACCCCTGAAGCTGGTCAGGGCATTCAATATACTTTTCCTTCTGCACCTGGATTTGCATATGACAGCTCCCAACAATTGAATATGGCATTTTCTCACCCACAGAGCTCACAAGTACAAAACAATGCTCCCTTTTCAAGTGTGATGCAGGGATATACGAATTCGTTGCCTAGCACTCTATTGGCTTCAAATGCTCAGAATGTAAGGGAGGATCTTCCATACTCACCTTTCCCTGTGACACAATCTATGCCTACAAAGTATAGCAATGTGCCTTCTTCCATCAGCGGTCCCACCATGTCCATGCCAGAGGCTTTGAGAGCAGGTGGTATCTCTTCTGCTCAACCTACTCAACAGACCCTACCTGGTGCTGGTGTCGCGACTGGACCTGCTCTTCCACAACAGCTGTTGCACCCTTACTCTCAACCTAGTCTTCCTTTGGGACATTTTTCCAACATGATTGGTTATCCTTTCTTGCCTCAGAGCTACACATATATGCCATCAGCATTCCAGCAAGCTTTTGCTGGTAACAGCACATACCATCAGTCGCTGGCTGCAGTTCTCCCACAGTATAAAAATAGTGTTTCTGTCAGCAGTTTGCCCCAGTCTGCTAATATTCCCTCTGGTTATGGGTTTGGTAGTTCAAGTAACATTCCTGGAGGAAACTTTCCTCTGAATCCACCGTCGGCACCTACTGGTACAACAATTGGTTATGATGATGTTATAAGCTCTCAGTACAAGGACAGTAACCATTTGATGTCACTTCAGCAGAATGACAACTCAGGCATGTGGGTTCACGGACCTGGTTCCCGAGCAATGCCCGCTGTTCCAGCCAGCAATTATTACAGCTTCCAGGGGCAGAATCAACAGCATGCTGGTTTCCGGCAAACCCAGCAACCGTCGCAGCAATTTGCTGGAGCTCTAAACTACCCGAACTTCTATCATTCTCAGACAGGGATGTCCCTCGATCATCAACAGCAAAGTTCAAGGGACGCATCCCTGGGTGGCTCACAGGGACAACCTTCTAAGCAGTCTCAACAATTATGGCAAAACAGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

835

Amino Acids

88.93

Weight (kDa)

5.22

Isoelectric Point (pI)

62.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 18 - 77 9.3e-34 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2171
AarI CACCTGC 3 cut(s) 403, 790, 1760
AasI GACNNNNNNGTC 1 cut(s) 2404
Acc36I ACCTGC 6 cut(s) 376, 395, 403, 790, 1760, 1843
AccB1I GGYRCC 5 cut(s) 571, 628, 667, 778, 2134
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 364
AccII CGCG 1 cut(s) 1826
AciI CCGC 7 cut(s) 6, 15, 18, 318, 617, 1734, 2268
AclWI GGATC 4 cut(s) 182, 328, 467, 1668
AcsI RAATTY 3 cut(s) 694, 787, 1609
AcuI CTGAAG 4 cut(s) 1149, 1404, 1479, 2195
AcvI CACGTG 1 cut(s) 314
AfaI GTAC 4 cut(s) 374, 1566, 2146, 2183
AfiI CCNNNNNNNGG 6 cut(s) 73, 545, 680, 774, 1463, 1817
AgsI TTSAA 9 cut(s) 88, 276, 588, 1478, 1531, 1587, 1638, 2091, 2433
AjnI CCWGG 8 cut(s) 574, 748, 1498, 1810, 2101, 2248, 2298, 2445
AjuI GAANNNNNNNTTGG 2 cut(s) 1250, 1282
AloI GAACNNNNNNTCC 2 cut(s) 1362, 1394
Alw21I GWGCWC 5 cut(s) 798, 1123, 1247, 1559, 2368
Alw26I GTCTC 6 cut(s) 179, 261, 863, 1021, 1268, 2484
AlwI GGATC 4 cut(s) 182, 328, 467, 1668
AlwNI CAGNNNCTG 1 cut(s) 1996
Ama87I CYCGRG 1 cut(s) 2256
AoxI GGCC 4 cut(s) 614, 728, 1222, 1392
ApeKI GCWGC 9 cut(s) 308, 422, 482, 677, 1076, 1087, 1090, 2000, 2350
ApoI RAATTY 3 cut(s) 694, 787, 1609
Asp700I GAANNNNTTC 1 cut(s) 523
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 5 cut(s) 308, 521, 1157, 1538, 1665
AsuII TTCGAA 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 2256
AvaII GGWCC 7 cut(s) 63, 151, 923, 1218, 1736, 1832, 2246
BaeGI GKGCMC 4 cut(s) 670, 781, 822, 1311
BanI GGYRCC 5 cut(s) 571, 628, 667, 778, 2134
BanII GRGCYC 5 cut(s) 714, 798, 1247, 1559, 2368
BauI CACGAG 1 cut(s) 258
BbrPI CACGTG 1 cut(s) 314
BbsI GAAGAC 2 cut(s) 1137, 1871
Bbv12I GWGCWC 5 cut(s) 798, 1123, 1247, 1559, 2368
BbvI GCAGC 9 cut(s) 295, 434, 494, 689, 1063, 1099, 1102, 1987, 2362
BceAI ACGGC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 1597, 1631
BciT130I CCWGG 8 cut(s) 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
BciVI GTATCC 1 cut(s) 677
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 6 cut(s) 179, 261, 863, 1021, 1268, 2484
BfaI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1620, 1875
BfmI CTRYAG 7 cut(s) 420, 466, 675, 1149, 1281, 1299, 2001
BfuAI ACCTGC 6 cut(s) 376, 395, 403, 790, 1760, 1843
BfuI GTATCC 1 cut(s) 677
BglII AGATCT 1 cut(s) 120
BlpI GCTNAGC 1 cut(s) 1053
Bme1390I CCNGG 9 cut(s) 67, 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
Bme18I GGWCC 7 cut(s) 63, 151, 923, 1218, 1736, 1832, 2246
BmeT110I CYCGRG 1 cut(s) 2256
BmrFI CCNGG 9 cut(s) 67, 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
BmrI ACTGGG 2 cut(s) 1398, 2044
BmsI GCATC 7 cut(s) 751, 983, 1051, 1306, 1357, 1584, 2450
BmuI ACTGGG 2 cut(s) 1398, 2044
BpiI GAAGAC 2 cut(s) 1137, 1871
BpmI CTGGAG 5 cut(s) 732, 1167, 1422, 2124, 2382
Bpu10I CCTNAGC 1 cut(s) 1227
Bpu1102I GCTNAGC 1 cut(s) 1053
Bpu14I TTCGAA 1 cut(s) 692
BpuEI CTTGAG 4 cut(s) 168, 466, 1058, 1219
BpuMI CCSGG 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 1347
BsaI GGTCTC 1 cut(s) 261
BsaJI CCNNGG 6 cut(s) 624, 714, 822, 2299, 2445, 2446
BsaWI WCCGGW 2 cut(s) 938, 1215
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 545, 680, 774, 1463, 1817
Bse1I ACTGG 9 cut(s) 43, 439, 476, 1253, 1393, 1395, 1834, 2050, 2146
Bse3DI GCAATG 3 cut(s) 28, 1720, 2268
Bse8I GATNNNNATC 1 cut(s) 1347
BseBI CCWGG 8 cut(s) 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
BseDI CCNNGG 6 cut(s) 624, 714, 822, 2299, 2445, 2446
BseGI GGATG 8 cut(s) 461, 527, 742, 1018, 1114, 1249, 2409, 2441
BseJI GATNNNNATC 1 cut(s) 1347
BseLI CCNNNNNNNGG 6 cut(s) 73, 545, 680, 774, 1463, 1817
BseMI GCAATG 3 cut(s) 28, 1720, 2268
BseNI ACTGG 9 cut(s) 43, 439, 476, 1253, 1393, 1395, 1834, 2050, 2146
BseSI GKGCMC 4 cut(s) 670, 781, 822, 1311
BseXI GCAGC 9 cut(s) 295, 434, 494, 689, 1063, 1099, 1102, 1987, 2362
BseYI CCCAGC 2 cut(s) 59, 2337
BsgI GTGCAG 3 cut(s) 91, 294, 1479
Bsh1236I CGCG 1 cut(s) 1826
BshFI GGCC 4 cut(s) 616, 730, 1224, 1394
BshNI GGYRCC 5 cut(s) 571, 628, 667, 778, 2134
BsiHKAI GWGCWC 5 cut(s) 798, 1123, 1247, 1559, 2368
BsiHKCI CYCGRG 1 cut(s) 2256
BsiSI CCGG 5 cut(s) 67, 939, 1216, 1221, 2329
BslFI GGGAC 6 cut(s) 49, 1722, 1902, 2392, 2450, 2475
BslI CCNNNNNNNGG 6 cut(s) 73, 545, 680, 774, 1463, 1817
BsmAI GTCTC 6 cut(s) 179, 261, 863, 1021, 1268, 2484
BsmBI CGTCTC 2 cut(s) 179, 863
BsmFI GGGAC 6 cut(s) 49, 1722, 1902, 2392, 2450, 2475
BsmI GAATGC 2 cut(s) 1473, 1952
BsnI GGCC 4 cut(s) 616, 730, 1224, 1394
Bso31I GGTCTC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 2256
Bsp119I TTCGAA 1 cut(s) 692
Bsp143I GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1660, 2413
Bsp1720I GCTNAGC 1 cut(s) 1053
Bsp68I TCGCGA 1 cut(s) 1826
BspACI CCGC 7 cut(s) 6, 15, 18, 318, 617, 1734, 2268
BspANI GGCC 4 cut(s) 616, 730, 1224, 1394
BspFNI CGCG 1 cut(s) 1826
BspHI TCATGA 1 cut(s) 655
BspMAI CTGCAG 4 cut(s) 424, 679, 1303, 2005
BspMI ACCTGC 6 cut(s) 376, 395, 403, 790, 1760, 1843
BspPI GGATC 4 cut(s) 182, 328, 467, 1668
BspQI GCTCTTC 2 cut(s) 200, 1845
BspT104I TTCGAA 1 cut(s) 692
BspT107I GGYRCC 5 cut(s) 571, 628, 667, 778, 2134
BspTNI GGTCTC 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 3 cut(s) 28, 1720, 2268
BsrI ACTGG 9 cut(s) 43, 439, 476, 1253, 1393, 1395, 1834, 2050, 2146
BssECI CCNNGG 6 cut(s) 624, 714, 822, 2299, 2445, 2446
BssMI GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1660, 2413
BssSI CACGAG 1 cut(s) 258
BssT1I CCWWGG 3 cut(s) 624, 714, 822
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 8 cut(s) 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
Bst4CI ACNGT 6 cut(s) 143, 470, 2016, 2130, 2192, 2346
Bst6I CTCTTC 5 cut(s) 200, 421, 1266, 1786, 1845
BstAPI GCANNNNNTGC 1 cut(s) 674
BstBAI YACGTR 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 692
BstC8I GCNNGC 9 cut(s) 427, 621, 818, 1193, 1962, 1998, 2268, 2281, 2319
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 8 cut(s) 461, 527, 742, 1018, 1114, 1249, 2409, 2441
BstFNI CGCG 1 cut(s) 1826
BstKTI GATC 8 cut(s) 123, 190, 301, 323, 475, 1237, 1663, 2416
BstMAI GTCTC 6 cut(s) 179, 261, 863, 1021, 1268, 2484
BstMBI GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1660, 2413
BstNI CCWGG 8 cut(s) 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
BstNSI RCATGY 3 cut(s) 763, 2236, 2321
BstSCI CCNGG 9 cut(s) 65, 574, 748, 1498, 1810, 2101, 2248, 2298, 2445
BstSFI CTRYAG 7 cut(s) 420, 466, 675, 1149, 1281, 1299, 2001
BstSLI GKGCMC 4 cut(s) 670, 781, 822, 1311
BstUI CGCG 1 cut(s) 1826
BstV1I GCAGC 9 cut(s) 295, 434, 494, 689, 1063, 1099, 1102, 1987, 2362
BstV2I GAAGAC 2 cut(s) 1137, 1871
BstX2I RGATCY 3 cut(s) 120, 187, 1660
BstYI RGATCY 3 cut(s) 120, 187, 1660
BsuI GTATCC 1 cut(s) 677
BsuRI GGCC 4 cut(s) 616, 730, 1224, 1394
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 8 cut(s) 461, 527, 742, 1018, 1114, 1249, 2409, 2441
BtsIMutI CAGTG 7 cut(s) 108, 475, 483, 902, 1260, 1402, 1428
BtuMI TCGCGA 1 cut(s) 1826
BveI ACCTGC 6 cut(s) 376, 395, 403, 790, 1760, 1843
Cac8I GCNNGC 9 cut(s) 427, 621, 818, 1193, 1962, 1998, 2268, 2281, 2319
CaiI CAGNNNCTG 1 cut(s) 1996
CciI TCATGA 1 cut(s) 655
CpoI CGGWCCG 1 cut(s) 1218
CseI GACGC 1 cut(s) 2447
CsiI ACCWGGT 2 cut(s) 1810, 2248
Csp6I GTAC 4 cut(s) 373, 1565, 2145, 2182
CspI CGGWCCG 1 cut(s) 1218
CviQI GTAC 4 cut(s) 373, 1565, 2145, 2182
DpnI GATC 8 cut(s) 122, 189, 300, 322, 474, 1236, 1662, 2415
DpnII GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1660, 2413
DrdI GACNNNNNNGTC 1 cut(s) 2404
DseDI GACNNNNNNGTC 1 cut(s) 2404
Eam1104I CTCTTC 5 cut(s) 200, 421, 1266, 1786, 1845
EarI CTCTTC 5 cut(s) 200, 421, 1266, 1786, 1845
EciI GGCGGA 1 cut(s) 333
Ecl136II GAGCTC 4 cut(s) 796, 1245, 1557, 2366
Eco130I CCWWGG 3 cut(s) 624, 714, 822
Eco24I GRGCYC 5 cut(s) 714, 798, 1247, 1559, 2368
Eco31I GGTCTC 1 cut(s) 261
Eco32I GATATC 2 cut(s) 551, 1006
Eco47I GGWCC 7 cut(s) 63, 151, 923, 1218, 1736, 1832, 2246
Eco53kI GAGCTC 4 cut(s) 796, 1245, 1557, 2366
Eco57I CTGAAG 4 cut(s) 1149, 1404, 1479, 2195
Eco72I CACGTG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 2256
EcoICRI GAGCTC 4 cut(s) 796, 1245, 1557, 2366
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 63
EcoRI GAATTC 2 cut(s) 694, 1609
EcoRII CCWGG 8 cut(s) 574, 748, 1498, 1810, 2101, 2248, 2298, 2445
EcoRV GATATC 2 cut(s) 551, 1006
EcoT14I CCWWGG 3 cut(s) 624, 714, 822
EcoT38I GRGCYC 5 cut(s) 714, 798, 1247, 1559, 2368
ErhI CCWWGG 3 cut(s) 624, 714, 822
Esp3I CGTCTC 2 cut(s) 179, 863
FaqI GGGAC 6 cut(s) 49, 1722, 1902, 2392, 2450, 2475
FauI CCCGC 1 cut(s) 2275
FauNDI CATATG 1 cut(s) 1510
FblI GTMKAC 1 cut(s) 364
FokI GGATG 8 cut(s) 448, 514, 729, 1005, 1121, 1236, 2416, 2428
FriOI GRGCYC 5 cut(s) 714, 798, 1247, 1559, 2368
FspBI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1620, 1875
GsaI CCCAGC 2 cut(s) 63, 2341
GsuI CTGGAG 5 cut(s) 732, 1167, 1422, 2124, 2382
HaeIII GGCC 4 cut(s) 616, 730, 1224, 1394
HapII CCGG 5 cut(s) 67, 939, 1216, 1221, 2329
HgaI GACGC 1 cut(s) 2447
HindIII AAGCTT 1 cut(s) 1962
HinfI GANTC 6 cut(s) 248, 344, 1156, 1412, 2122, 2308
HpaII CCGG 5 cut(s) 67, 939, 1216, 1221, 2329
HphI GGTGA 5 cut(s) 308, 521, 1157, 1538, 1665
Hpy166II GTNNAC 2 cut(s) 365, 2242
Hpy188I TCNGA 8 cut(s) 81, 793, 1315, 1366, 1647, 1929, 2121, 2397
Hpy188III TCNNGA 9 cut(s) 48, 185, 546, 656, 1022, 1184, 1328, 1825, 2256
Hpy8I GTNNAC 2 cut(s) 365, 2242
Hpy99I CGWCG 3 cut(s) 456, 2134, 2350
HpyAV CCTTC 8 cut(s) 22, 549, 601, 1099, 1428, 1500, 1731, 2478
HpyCH4III ACNGT 6 cut(s) 143, 470, 2016, 2130, 2192, 2346
HpyCH4IV ACGT 1 cut(s) 313
HpySE526I ACGT 1 cut(s) 313
KflI GGGWCCC 1 cut(s) 63
Kzo9I GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1660, 2413
LguI GCTCTTC 2 cut(s) 200, 1845
LmnI GCTCC 6 cut(s) 238, 1118, 1250, 1523, 1582, 2363
Lsp1109I GCAGC 9 cut(s) 295, 434, 494, 689, 1063, 1099, 1102, 1987, 2362
LweI GCATC 7 cut(s) 751, 983, 1051, 1306, 1357, 1584, 2450
MabI ACCWGGT 2 cut(s) 1810, 2248
MaeI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1620, 1875
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 8 cut(s) 143, 1262, 1425, 1684, 1973, 2093, 2192, 2205
MalI GATC 8 cut(s) 122, 189, 300, 322, 474, 1236, 1662, 2415
MbiI CCGCTC 1 cut(s) 6
MboI GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1660, 2413
MfeI CAATTG 2 cut(s) 1526, 2151
MflI RGATCY 3 cut(s) 120, 187, 1660
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 1923
MroXI GAANNNNTTC 1 cut(s) 523
MseI TTAA 4 cut(s) 135, 168, 603, 1442
MslI CAYNNNNRTG 3 cut(s) 1314, 1748, 1940
MspA1I CMGCKG 6 cut(s) 59, 425, 485, 1734, 1852, 2270
MspI CCGG 5 cut(s) 67, 939, 1216, 1221, 2329
MspR9I CCNGG 9 cut(s) 67, 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
MunI CAATTG 2 cut(s) 1526, 2151
Mva1269I GAATGC 2 cut(s) 1473, 1952
MvaI CCWGG 8 cut(s) 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
MvnI CGCG 1 cut(s) 1826
NciI CCSGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 1510
NdeII GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1660, 2413
NmeAIII GCCGAG 1 cut(s) 944
NmuCI GTSAC 4 cut(s) 1262, 1425, 1684, 2205
NruI TCGCGA 1 cut(s) 1826
NspI RCATGY 3 cut(s) 763, 2236, 2321
NspV TTCGAA 1 cut(s) 692
PaeI GCATGC 1 cut(s) 2321
PagI TCATGA 1 cut(s) 655
PaqCI CACCTGC 3 cut(s) 403, 790, 1760
PasI CCCWGGG 1 cut(s) 2446
PciSI GCTCTTC 2 cut(s) 200, 1845
PctI GAATGC 2 cut(s) 1473, 1952
PdmI GAANNNNTTC 1 cut(s) 523
PfeI GAWTC 5 cut(s) 248, 1156, 1412, 2122, 2308
PfoI TCCNGGA 2 cut(s) 65, 2101
PleI GAGTC 1 cut(s) 352
PmaCI CACGTG 1 cut(s) 314
PmlI CACGTG 1 cut(s) 314
PpsI GAGTC 1 cut(s) 352
Ppu21I YACGTR 1 cut(s) 314
PpuMI RGGWCCY 1 cut(s) 63
PsiI TTATAA 1 cut(s) 2171
Psp124BI GAGCTC 4 cut(s) 798, 1247, 1559, 2368
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 8 cut(s) 574, 748, 1498, 1810, 2101, 2248, 2298, 2445
PspCI CACGTG 1 cut(s) 314
PspFI CCCAGC 2 cut(s) 59, 2337
PspGI CCWGG 8 cut(s) 574, 748, 1498, 1810, 2101, 2248, 2298, 2445
PspPPI RGGWCCY 1 cut(s) 63
PstI CTGCAG 4 cut(s) 424, 679, 1303, 2005
PstNI CAGNNNCTG 1 cut(s) 1996
PsuI RGATCY 3 cut(s) 120, 187, 1660
PvuII CAGCTG 4 cut(s) 59, 425, 485, 1852
RruI TCGCGA 1 cut(s) 1826
RsaI GTAC 4 cut(s) 374, 1566, 2146, 2183
RsaNI GTAC 4 cut(s) 373, 1565, 2145, 2182
RseI CAYNNNNRTG 3 cut(s) 1314, 1748, 1940
Rsr2I CGGWCCG 1 cut(s) 1218
RsrII CGGWCCG 1 cut(s) 1218
SacI GAGCTC 4 cut(s) 798, 1247, 1559, 2368
SapI GCTCTTC 2 cut(s) 200, 1845
SaqAI TTAA 4 cut(s) 135, 168, 603, 1442
Sau3AI GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1660, 2413
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 9 cut(s) 67, 576, 750, 1500, 1812, 2103, 2250, 2300, 2447
SexAI ACCWGGT 2 cut(s) 1810, 2248
SfaNI GCATC 7 cut(s) 751, 983, 1051, 1306, 1357, 1584, 2450
SfcI CTRYAG 7 cut(s) 420, 466, 675, 1149, 1281, 1299, 2001
SfuI TTCGAA 1 cut(s) 692
SinI GGWCC 7 cut(s) 63, 151, 923, 1218, 1736, 1832, 2246
SmiMI CAYNNNNRTG 3 cut(s) 1314, 1748, 1940
SmlI CTYRAG 4 cut(s) 183, 445, 1037, 1198
SmoI CTYRAG 4 cut(s) 183, 445, 1037, 1198
SphI GCATGC 1 cut(s) 2321
SsiI CCGC 7 cut(s) 6, 15, 18, 318, 617, 1734, 2268
SspI AATATT 2 cut(s) 271, 2062
SspMI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1620, 1875
SstI GAGCTC 4 cut(s) 798, 1247, 1559, 2368
StyD4I CCNGG 9 cut(s) 65, 574, 748, 1498, 1810, 2101, 2248, 2298, 2445
StyI CCWWGG 3 cut(s) 624, 714, 822
TaaI ACNGT 6 cut(s) 143, 470, 2016, 2130, 2192, 2346
TaiI ACGT 1 cut(s) 316
TaqI TCGA 5 cut(s) 692, 891, 897, 1241, 2412
TatI WGTACW 2 cut(s) 1564, 2181
TauI GCSGC 3 cut(s) 18, 21, 619
TfiI GAWTC 5 cut(s) 248, 1156, 1412, 2122, 2308
Tru1I TTAA 4 cut(s) 135, 168, 603, 1442
Tru9I TTAA 4 cut(s) 135, 168, 603, 1442
TscAI CASTG 7 cut(s) 115, 475, 483, 909, 1260, 1402, 1428
TseFI GTSAC 4 cut(s) 1262, 1425, 1684, 2205
TseI GCWGC 9 cut(s) 308, 422, 482, 677, 1076, 1087, 1090, 2000, 2350
Tsp45I GTSAC 4 cut(s) 1262, 1425, 1684, 2205
TspDTI ATGAA 9 cut(s) 174, 213, 516, 687, 798, 1056, 1062, 1166, 1370
TspGWI ACGGA 4 cut(s) 260, 531, 887, 2259
TspRI CASTG 7 cut(s) 115, 475, 483, 909, 1260, 1402, 1428
VpaK11BI GGWCC 7 cut(s) 63, 151, 923, 1218, 1736, 1832, 2246
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 3 cut(s) 694, 787, 1609
XceI RCATGY 3 cut(s) 763, 2236, 2321
XmiI GTMKAC 1 cut(s) 364
XmnI GAANNNNTTC 1 cut(s) 523
XspI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1620, 1875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.