MD05G1291000.v1.1

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006562
Physical Location & Seq
Reverse (-)
42265193 .. 42271619
6427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1291000.v1.1.491

Sequence Viewer

Length: 2571 bp
ATGAGCGGGAGAGGCGGCGGAGGCGTGGCCAGCAATGGTAAGGGCAATACTGGGATTTCCGGCATACCTGCGGGGTCTCGGAAGATGGTGCAGAGCTTGAAGGAGATTGTGAATAACTGCACTGAGCAGGAGATCTACGCTATGCTTAAAGACTGTAACATGGACCCTAACGAAGCCGTCAATCGCCTCCTCGCCCAAGATCCTTTTCATGAAGTGAAGAGCAAACGAGAAAAGAAAAAAGAGAGTAAGGAGCCGACAGATTCCAGGTCTCGTGGGGCGAGTAACGCTTCAAGTTATGGTGCTAGAGGTGGTGACCGTTATTCTGCACGTGGCGGATCAAACCACTTTAGCTCTAATGATTCTGGTTTTTCGCATGGTAAGTCTGCATACAAGAAGGAAAATGGGACACATGCTTATCCAGGTTCTGCGTCTGGCATGGCAGGAAATCACACAGGTCGACGACCCACATCCTACAGCGATTCTGTGGGGAGTGAAAATAAAATGCCAACATTGAGCACAGATGATGGAATATCGTCATTCTCACAGCCTTCTTCAGGATATCAGTCTGCATGGGGAGGGGTTCCAGGTCAAGTATCCATGGCCGACATTGTTAAAATGGGCAGGCCGCAAGCCAAGGCTACCACCATGCCAAACCCCCATAATCATAGTGGTAATCATCATGATGGGGTGCCTCCTGCAGCATCATTGCATCACAATTTGCATTCACCGCAAGATCATGTTCCAAAGTTGTCAGCGACCCATGATGAGTGGCCCTTGATTGATCCACCATCTGTTAGCATGTCCTCTGTTCTTGGGGCACCCGCAAACTCTGAGCTGTATGCTGATTCATCTAACGTGCCCGTCGATAGAGCTAATCAACATATAAGATCCCAGATAGATGAGGTTGAGGTGGAAGAAGATGATTCTGTTGAGGCACTTCCAAGCCAGAATGAGCCTACTTCTGTATCTGGTAGACATTTACAAGAAGATAATTCCGGAGGTGCATCTAATTTTGATAATAGCTTGTATGAAGACATAAATTCCTACCAACCACAGCGGCATGCTTTCGAGGAGAATGAAGCTGAAGAAGATGTGTCATCAGTGGCTGCAAACTTGCAGCAACTTAATTTACAAAACGATGATAGGGGAGCAGCTCCTGAAGAAGAAAAACCTCCTGTGGTCATTCCTAATCACCTTCAGCTTCATACTCCAGATGTCTTGAACTTGAGCTTTGGAAGTTTCCGATCTGGCCCAGATCCTGCTCTTCCTAGCTCCGATTCATTTGCATCGGAGCCACAAAAAAGAAACTTTGAAGAGACATCTGGGGCCGTAGATGTTTCTGCAATTGCACACTCAGATGCAAGAAATCCCGAGTATTATGGAGATGAGCATCTCATGAATGCCTCGGACGAAAGTCTAGTTCATAGAACTGTAGCCAGTGCGGGAGATTATGAATCTCCTTCAGTTTCACAAGCAGAGATCTTGAAACAAGAAACCCATGAACCTGATCAGGGGAATCAATATGTGTTCTCTTCTGCACCTGGATTTGCATATGAGAATTCCCAACAATTGGATGCGGCATTTTCTCACCAACATACAAGCTCGCAGATGCAGAATATGGCTCCTTTTTCGAATGTGATGGCATATACAAATTCATTGCCCAGCACTCTGTTAGCTTCGAGTGCTCAAGCTGCAAGGGAGGATTTTCCATACTCACCGTTCCCCATGACCCAATCTATGCCCACAAAGTATGGCAATGCGGCTTCTTCTATCAGCGGTCCCACCATCTCCATGGCAGAGGCATTGAGAGCAGGTGGTATTTCTGCACCTCAGCCTACTCCACAGGGCCTGCCTGGTGCTAATGTTGCTACTGGACCAGCTCTTCCACAACACCTTGCAGTGCACCCTTATTCTCAACCTACTCTTCCGCTAGGGCATTTTTCAAACATGATCGGTTATCCTTTCCTGCCTCAGAGCTACACATATATGCCATCAGCTTTCCAGCAACAATTTGCTGGTAACAGCACATATCATCAGTCTCTGGCTGCAGTTCTCCCACAGTATAAAAATAGCGTCTCTGTTAGCAGTTTGCCTCAGTCTGCTAATATTCCGCCTGGCTATGGGTTCAGTAGTTCAACCAGCATTCCTGGTGGAAACTTTCCTTTGAATCCACCTTCGGCACCTACAGGAACAACGATGGGCTATGACGATGTCGTAAACTCTCAGTACAAGGACAATAGCCATTTGATCTCACTTCAGCAGCAAAATGATAACTCGGGCATGTGGGTTCATGGACCTGGTTCCCGAGCAATGTCTGCTGTTCCAGCCAGCAACTATTACAGCTTTCAGGGGCAGAATCAACAGCACGCAGGGTTCCGGCAAGCTCAGCAACCACCGCCTTCACAGCAGTATGCTGGAACTCTCGGTTACCCCAACTTTTATCACTCTCAAACCGGGATGTCCCTAGAACACCAGCAGCAGCAAAGTTCGAGGGACGCGTCCCTGGGCAGCTCACAAGGCGGCCAACCCTCGAAGCAGTCGCAACAGCAGCTATGGCAGAACAGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

857

Amino Acids

91.67

Weight (kDa)

5.4

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 22 - 81 9.6e-34 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1802
Acc36I ACCTGC 2 cut(s) 76, 1802
AccB1I GGYRCC 3 cut(s) 688, 817, 2179
AccB7I CCANNNNNTGG 1 cut(s) 1570
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 2 cut(s) 457, 973
AccII CGCG 1 cut(s) 2498
AccIII TCCGGA 1 cut(s) 995
AclWI GGATC 5 cut(s) 194, 343, 776, 882, 1250
AcoI YGGCCR 3 cut(s) 27, 600, 2521
AcsI RAATTY 3 cut(s) 1039, 1558, 1651
AcuI CTGAAG 6 cut(s) 537, 1104, 1179, 1181, 1446, 2240
AcvI CACGTG 1 cut(s) 329
AfaI GTAC 1 cut(s) 2228
AfiI CCNNNNNNNGG 4 cut(s) 554, 1511, 1570, 2120
AflIII ACRYGT 1 cut(s) 2496
AgsI TTSAA 8 cut(s) 100, 291, 1222, 1313, 1486, 1944, 2136, 2167
AjnI CCWGG 9 cut(s) 263, 418, 583, 1540, 1852, 2113, 2146, 2296, 2502
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
Alw21I GWGCWC 3 cut(s) 518, 1687, 1905
Alw26I GTCTC 5 cut(s) 81, 273, 1310, 2043, 2080
Alw44I GTGCAC 1 cut(s) 1901
AlwI GGATC 5 cut(s) 194, 343, 776, 882, 1250
AlwNI CAGNNNCTG 6 cut(s) 425, 1106, 1157, 1259, 1849, 2041
Ama87I CYCGRG 3 cut(s) 1370, 2275, 2304
Aor13HI TCCGGA 1 cut(s) 995
AoxI GGCC 8 cut(s) 27, 600, 623, 770, 1249, 1326, 1846, 2521
ApaLI GTGCAC 1 cut(s) 1901
ApoI RAATTY 3 cut(s) 1039, 1558, 1651
AspS9I GGNCC 8 cut(s) 163, 771, 1250, 1326, 1778, 1846, 1874, 2294
AsuC2I CCSGG 1 cut(s) 2455
AsuHPI GGTGA 5 cut(s) 323, 717, 1184, 1580, 1707
AsuII TTCGAA 1 cut(s) 1631
AvaI CYCGRG 3 cut(s) 1370, 2275, 2304
AvaII GGWCC 4 cut(s) 163, 1778, 1874, 2294
BaeGI GKGCMC 3 cut(s) 820, 861, 1905
BaeI ACNNNNGTAYC 2 cut(s) 948, 981
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 3 cut(s) 688, 817, 2179
BauI CACGAG 1 cut(s) 270
BbrPI CACGTG 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 1038
Bbv12I GWGCWC 3 cut(s) 518, 1687, 1905
BbvCI CCTCAGC 1 cut(s) 1830
BccI CCATC 8 cut(s) 79, 518, 677, 796, 1633, 1793, 1999, 2191
BceAI ACGGC 2 cut(s) 161, 1313
BcgI CGANNNNNNTGC 2 cut(s) 1265, 1299
BciT130I CCWGG 9 cut(s) 265, 420, 585, 1542, 1854, 2115, 2148, 2298, 2504
BciVI GTATCC 1 cut(s) 604
BclI TGATCA 1 cut(s) 1507
BcnI CCSGG 1 cut(s) 2455
BcoDI GTCTC 5 cut(s) 81, 273, 1310, 2043, 2080
BfaI CTAG 5 cut(s) 303, 1269, 1418, 1931, 2465
BfmI CTRYAG 5 cut(s) 472, 696, 1431, 2046, 2184
BfuAI ACCTGC 2 cut(s) 76, 1802
BfuI GTATCC 1 cut(s) 604
BglII AGATCT 2 cut(s) 132, 1479
BlpI GCTNAGC 1 cut(s) 2385
Bme18I GGWCC 4 cut(s) 163, 1778, 1874, 2294
BmeT110I CYCGRG 3 cut(s) 1370, 2275, 2304
BmgT120I GGNCC 8 cut(s) 163, 771, 1250, 1326, 1778, 1846, 1874, 2294
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 8 cut(s) 710, 718, 1013, 1295, 1348, 1399, 1564, 1599
BmuI ACTGGG 1 cut(s) 60
BoxI GACNNNNGTC 1 cut(s) 1413
BpiI GAAGAC 1 cut(s) 1038
BpmI CTGGAG 1 cut(s) 1194
Bpu10I CCTNAGC 1 cut(s) 1830
Bpu1102I GCTNAGC 1 cut(s) 2385
Bpu14I TTCGAA 1 cut(s) 1631
BpuEI CTTGAG 2 cut(s) 1246, 1671
BpuMI CCSGG 1 cut(s) 2455
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 1389
BsaI GGTCTC 2 cut(s) 81, 273
BsaJI CCNNGG 6 cut(s) 597, 633, 1404, 1791, 2502, 2503
BsaWI WCCGGW 1 cut(s) 995
Bsc4I CCNNNNNNNGG 4 cut(s) 554, 1511, 1570, 2120
Bse1I ACTGG 3 cut(s) 55, 1437, 1876
Bse3DI GCAATG 5 cut(s) 40, 704, 1655, 1762, 2316
Bse8I GATNNNNATC 1 cut(s) 1389
BseAI TCCGGA 1 cut(s) 995
BseBI CCWGG 9 cut(s) 265, 420, 585, 1542, 1854, 2115, 2148, 2298, 2504
BseDI CCNNGG 6 cut(s) 597, 633, 1404, 1791, 2502, 2503
BseGI GGATG 3 cut(s) 467, 1579, 2463
BseJI GATNNNNATC 1 cut(s) 1389
BseLI CCNNNNNNNGG 4 cut(s) 554, 1511, 1570, 2120
BseMI GCAATG 5 cut(s) 40, 704, 1655, 1762, 2316
BseMII CTCAG 8 cut(s) 114, 822, 1368, 1844, 1985, 2108, 2237, 2399
BseNI ACTGG 3 cut(s) 55, 1437, 1876
BseRI GAGGAG 2 cut(s) 179, 1085
BseSI GKGCMC 3 cut(s) 820, 861, 1905
BseYI CCCAGC 1 cut(s) 1661
BsgI GTGCAG 5 cut(s) 103, 110, 309, 1521, 1809
Bsh1236I CGCG 1 cut(s) 2498
BshFI GGCC 8 cut(s) 29, 602, 625, 772, 1251, 1328, 1848, 2523
BshNI GGYRCC 3 cut(s) 688, 817, 2179
BsiHKAI GWGCWC 3 cut(s) 518, 1687, 1905
BsiHKCI CYCGRG 3 cut(s) 1370, 2275, 2304
BsiSI CCGG 4 cut(s) 60, 996, 2377, 2454
BslFI GGGAC 5 cut(s) 418, 1764, 2446, 2485, 2507
BslI CCNNNNNNNGG 4 cut(s) 554, 1511, 1570, 2120
BsmAI GTCTC 5 cut(s) 81, 273, 1310, 2043, 2080
BsmBI CGTCTC 1 cut(s) 2080
BsmFI GGGAC 5 cut(s) 418, 1764, 2446, 2485, 2507
BsmI GAATGC 3 cut(s) 721, 1405, 2142
BsnI GGCC 8 cut(s) 29, 602, 625, 772, 1251, 1328, 1848, 2523
Bso31I GGTCTC 2 cut(s) 81, 273
BsoBI CYCGRG 3 cut(s) 1370, 2275, 2304
Bsp119I TTCGAA 1 cut(s) 1631
Bsp1286I GDGCHC 5 cut(s) 518, 820, 861, 1687, 1905
Bsp13I TCCGGA 1 cut(s) 995
Bsp1720I GCTNAGC 1 cut(s) 2385
Bsp19I CCATGG 2 cut(s) 597, 1791
BspANI GGCC 8 cut(s) 29, 602, 625, 772, 1251, 1328, 1848, 2523
BspCNI CTCAG 8 cut(s) 115, 823, 1367, 1843, 1984, 2107, 2236, 2398
BspEI TCCGGA 1 cut(s) 995
BspFNI CGCG 1 cut(s) 2498
BspHI TCATGA 3 cut(s) 208, 679, 1395
BspMAI CTGCAG 2 cut(s) 700, 2050
BspMI ACCTGC 2 cut(s) 76, 1802
BspPI GGATC 5 cut(s) 194, 343, 776, 882, 1250
BspQI GCTCTTC 3 cut(s) 212, 1269, 1887
BspT104I TTCGAA 1 cut(s) 1631
BspT107I GGYRCC 3 cut(s) 688, 817, 2179
BspTNI GGTCTC 2 cut(s) 81, 273
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 5 cut(s) 40, 704, 1655, 1762, 2316
BsrI ACTGG 3 cut(s) 55, 1437, 1876
BssECI CCNNGG 6 cut(s) 597, 633, 1404, 1791, 2502, 2503
BssSI CACGAG 1 cut(s) 270
BssT1I CCWWGG 3 cut(s) 597, 633, 1791
Bst2BI CACGAG 1 cut(s) 270
Bst2UI CCWGG 9 cut(s) 265, 420, 585, 1542, 1854, 2115, 2148, 2298, 2504
Bst4CI ACNGT 5 cut(s) 155, 317, 1432, 1719, 2061
Bst6I CTCTTC 6 cut(s) 212, 1269, 1308, 1537, 1887, 1929
BstAPI GCANNNNNTGC 1 cut(s) 2315
BstBAI YACGTR 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 1631
BstC8I GCNNGC 9 cut(s) 31, 623, 630, 1062, 1604, 1850, 2329, 2367, 2382
BstDEI CTNAG 8 cut(s) 123, 831, 1354, 1830, 1971, 2094, 2223, 2385
BstDSI CCRYGG 2 cut(s) 597, 1791
BstEII GGTNACC 2 cut(s) 311, 2426
BstENI CCTNNNNNAGG 1 cut(s) 552
BstF5I GGATG 3 cut(s) 467, 1579, 2463
BstFNI CGCG 1 cut(s) 2498
BstMAI GTCTC 5 cut(s) 81, 273, 1310, 2043, 2080
BstNI CCWGG 9 cut(s) 265, 420, 585, 1542, 1854, 2115, 2148, 2298, 2504
BstNSI RCATGY 4 cut(s) 413, 802, 1064, 2284
BstPAI GACNNNNGTC 1 cut(s) 1413
BstPI GGTNACC 2 cut(s) 311, 2426
BstSFI CTRYAG 5 cut(s) 472, 696, 1431, 2046, 2184
BstSLI GKGCMC 3 cut(s) 820, 861, 1905
BstUI CGCG 1 cut(s) 2498
BstV2I GAAGAC 1 cut(s) 1038
BstX2I RGATCY 5 cut(s) 132, 199, 887, 1255, 1479
BstXI CCANNNNNNTGG 1 cut(s) 1792
BstYI RGATCY 5 cut(s) 132, 199, 887, 1255, 1479
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 8 cut(s) 29, 602, 625, 772, 1251, 1328, 1848, 2523
BtgI CCRYGG 2 cut(s) 597, 1791
BtsCI GGATG 3 cut(s) 467, 1579, 2463
BtsI GCAGTG 1 cut(s) 1905
BtsIMutI CAGTG 4 cut(s) 120, 1107, 1444, 1905
BveI ACCTGC 2 cut(s) 76, 1802
Cac8I GCNNGC 9 cut(s) 31, 623, 630, 1062, 1604, 1850, 2329, 2367, 2382
CaiI CAGNNNCTG 6 cut(s) 425, 1106, 1157, 1259, 1849, 2041
CciI TCATGA 3 cut(s) 208, 679, 1395
Cfr13I GGNCC 8 cut(s) 163, 771, 1250, 1326, 1778, 1846, 1874, 2294
CseI GACGC 4 cut(s) 417, 2062, 2487, 2504
CsiI ACCWGGT 1 cut(s) 2296
Csp6I GTAC 1 cut(s) 2227
CviQI GTAC 1 cut(s) 2227
DdeI CTNAG 8 cut(s) 123, 831, 1354, 1830, 1971, 2094, 2223, 2385
EaeI YGGCCR 3 cut(s) 27, 600, 2521
Eam1104I CTCTTC 6 cut(s) 212, 1269, 1308, 1537, 1887, 1929
EarI CTCTTC 6 cut(s) 212, 1269, 1308, 1537, 1887, 1929
EciI GGCGGA 3 cut(s) 33, 348, 2100
Eco130I CCWWGG 3 cut(s) 597, 633, 1791
Eco31I GGTCTC 2 cut(s) 81, 273
Eco32I GATATC 1 cut(s) 560
Eco47I GGWCC 4 cut(s) 163, 1778, 1874, 2294
Eco57I CTGAAG 6 cut(s) 537, 1104, 1179, 1181, 1446, 2240
Eco72I CACGTG 1 cut(s) 329
Eco88I CYCGRG 3 cut(s) 1370, 2275, 2304
Eco91I GGTNACC 2 cut(s) 311, 2426
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 1846
EcoO65I GGTNACC 2 cut(s) 311, 2426
EcoRI GAATTC 1 cut(s) 1558
EcoRII CCWGG 9 cut(s) 263, 418, 583, 1540, 1852, 2113, 2146, 2296, 2502
EcoRV GATATC 1 cut(s) 560
EcoT14I CCWWGG 3 cut(s) 597, 633, 1791
ErhI CCWWGG 3 cut(s) 597, 633, 1791
Esp3I CGTCTC 1 cut(s) 2080
FaqI GGGAC 5 cut(s) 418, 1764, 2446, 2485, 2507
FauI CCCGC 3 cut(s) 64, 829, 1435
FauNDI CATATG 1 cut(s) 1552
FbaI TGATCA 1 cut(s) 1507
FblI GTMKAC 2 cut(s) 457, 973
FokI GGATG 3 cut(s) 454, 1586, 2470
FspBI CTAG 5 cut(s) 303, 1269, 1418, 1931, 2465
GsaI CCCAGC 1 cut(s) 1665
GsuI CTGGAG 1 cut(s) 1194
HaeIII GGCC 8 cut(s) 29, 602, 625, 772, 1251, 1328, 1848, 2523
HapII CCGG 4 cut(s) 60, 996, 2377, 2454
HgaI GACGC 4 cut(s) 417, 2062, 2487, 2504
HincII GTYRAC 1 cut(s) 458
HindII GTYRAC 1 cut(s) 458
HpaII CCGG 4 cut(s) 60, 996, 2377, 2454
HphI GGTGA 5 cut(s) 323, 717, 1184, 1580, 1707
Hpy166II GTNNAC 4 cut(s) 458, 974, 1903, 2218
Hpy188I TCNGA 8 cut(s) 81, 832, 1244, 1276, 1291, 1357, 1408, 1974
Hpy8I GTNNAC 4 cut(s) 458, 974, 1903, 2218
Hpy99I CGWCG 2 cut(s) 462, 866
HpyAV CCTTC 7 cut(s) 94, 388, 558, 1205, 1470, 2184, 2409
HpyCH4III ACNGT 5 cut(s) 155, 317, 1432, 1719, 2061
HpyCH4IV ACGT 2 cut(s) 328, 855
HpyF3I CTNAG 8 cut(s) 123, 831, 1354, 1830, 1971, 2094, 2223, 2385
HpySE526I ACGT 2 cut(s) 328, 855
Kpn2I TCCGGA 1 cut(s) 995
Ksp22I TGATCA 1 cut(s) 1507
LguI GCTCTTC 3 cut(s) 212, 1269, 1887
LmnI GCTCC 6 cut(s) 250, 1148, 1159, 1277, 1291, 1627
LweI GCATC 8 cut(s) 710, 718, 1013, 1295, 1348, 1399, 1564, 1599
MabI ACCWGGT 1 cut(s) 2296
MaeI CTAG 5 cut(s) 303, 1269, 1418, 1931, 2465
MaeII ACGT 2 cut(s) 328, 855
MaeIII GTNAC 5 cut(s) 155, 281, 311, 2018, 2426
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 2 cut(s) 1344, 1568
MflI RGATCY 5 cut(s) 132, 199, 887, 1255, 1479
MhlI GDGCHC 5 cut(s) 518, 820, 861, 1687, 1905
MlsI TGGCCA 1 cut(s) 29
MluI ACGCGT 1 cut(s) 2496
MluNI TGGCCA 1 cut(s) 29
Mox20I TGGCCA 1 cut(s) 29
MroI TCCGGA 1 cut(s) 995
MscI TGGCCA 1 cut(s) 29
MseI TTAA 3 cut(s) 147, 612, 1125
MslI CAYNNNNRTG 4 cut(s) 681, 1356, 1790, 1985
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 2 cut(s) 1057, 1776
MspI CCGG 4 cut(s) 60, 996, 2377, 2454
MunI CAATTG 2 cut(s) 1344, 1568
Mva1269I GAATGC 3 cut(s) 721, 1405, 2142
MvaI CCWGG 9 cut(s) 265, 420, 585, 1542, 1854, 2115, 2148, 2298, 2504
MvnI CGCG 1 cut(s) 2498
NciI CCSGG 1 cut(s) 2455
NcoI CCATGG 2 cut(s) 597, 1791
NdeI CATATG 1 cut(s) 1552
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 4 cut(s) 413, 802, 1064, 2284
NspV TTCGAA 1 cut(s) 1631
PaeI GCATGC 1 cut(s) 1064
PagI TCATGA 3 cut(s) 208, 679, 1395
PaqCI CACCTGC 1 cut(s) 1802
PasI CCCWGGG 1 cut(s) 2503
PciSI GCTCTTC 3 cut(s) 212, 1269, 1887
PcsI WCGNNNNNNNCGW 2 cut(s) 861, 2495
PctI GAATGC 3 cut(s) 721, 1405, 2142
PflFI GACNNNGTC 1 cut(s) 2210
PflMI CCANNNNNTGG 1 cut(s) 1570
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
Ppu21I YACGTR 1 cut(s) 329
PshAI GACNNNNGTC 1 cut(s) 1413
Psp6I CCWGG 9 cut(s) 263, 418, 583, 1540, 1852, 2113, 2146, 2296, 2502
PspCI CACGTG 1 cut(s) 329
PspEI GGTNACC 2 cut(s) 311, 2426
PspFI CCCAGC 1 cut(s) 1661
PspGI CCWGG 9 cut(s) 263, 418, 583, 1540, 1852, 2113, 2146, 2296, 2502
PspPI GGNCC 8 cut(s) 163, 771, 1250, 1326, 1778, 1846, 1874, 2294
PstI CTGCAG 2 cut(s) 700, 2050
PstNI CAGNNNCTG 6 cut(s) 425, 1106, 1157, 1259, 1849, 2041
PsuI RGATCY 5 cut(s) 132, 199, 887, 1255, 1479
PsyI GACNNNGTC 1 cut(s) 2210
RsaI GTAC 1 cut(s) 2228
RsaNI GTAC 1 cut(s) 2227
RseI CAYNNNNRTG 4 cut(s) 681, 1356, 1790, 1985
SalI GTCGAC 1 cut(s) 456
SapI GCTCTTC 3 cut(s) 212, 1269, 1887
SaqAI TTAA 3 cut(s) 147, 612, 1125
Sau96I GGNCC 8 cut(s) 163, 771, 1250, 1326, 1778, 1846, 1874, 2294
SduI GDGCHC 5 cut(s) 518, 820, 861, 1687, 1905
SexAI ACCWGGT 1 cut(s) 2296
SfaNI GCATC 8 cut(s) 710, 718, 1013, 1295, 1348, 1399, 1564, 1599
SfcI CTRYAG 5 cut(s) 472, 696, 1431, 2046, 2184
SfuI TTCGAA 1 cut(s) 1631
SinI GGWCC 4 cut(s) 163, 1778, 1874, 2294
SmiMI CAYNNNNRTG 4 cut(s) 681, 1356, 1790, 1985
SmlI CTYRAG 2 cut(s) 1225, 1686
SmoI CTYRAG 2 cut(s) 1225, 1686
SphI GCATGC 1 cut(s) 1064
SspI AATATT 1 cut(s) 2107
SspMI CTAG 5 cut(s) 303, 1269, 1418, 1931, 2465
StyI CCWWGG 3 cut(s) 597, 633, 1791
TaaI ACNGT 5 cut(s) 155, 317, 1432, 1719, 2061
TaiI ACGT 2 cut(s) 331, 858
TaqI TCGA 7 cut(s) 457, 864, 1068, 1631, 1679, 2489, 2531
TatI WGTACW 1 cut(s) 2226
TauI GCSGC 6 cut(s) 18, 628, 1060, 1580, 1763, 2523
Tru1I TTAA 3 cut(s) 147, 612, 1125
Tru9I TTAA 3 cut(s) 147, 612, 1125
TscAI CASTG 4 cut(s) 127, 1107, 1444, 1905
TseFI GTSAC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 311
TspRI CASTG 4 cut(s) 127, 1107, 1444, 1905
Tth111I GACNNNGTC 1 cut(s) 2210
Van91I CCANNNNNTGG 1 cut(s) 1570
VneI GTGCAC 1 cut(s) 1901
VpaK11BI GGWCC 4 cut(s) 163, 1778, 1874, 2294
XagI CCTNNNNNAGG 1 cut(s) 552
XapI RAATTY 3 cut(s) 1039, 1558, 1651
XceI RCATGY 4 cut(s) 413, 802, 1064, 2284
XcmI CCANNNNNNNNNTGG 2 cut(s) 665, 1789
XmiI GTMKAC 2 cut(s) 457, 973
XspI CTAG 5 cut(s) 303, 1269, 1418, 1931, 2465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.