RchiOBHm_Chr5g0013011

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006562
Physical Location & Seq
Forward (+)
8822850 .. 8829419
6570 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29356

Sequence Viewer

Length: 2505 bp
ATGAGCGGGAGAGGCGGCGGCAATGGGAAGGGCAATACTGGAATATCGGGAATACCAGCTGGGTCCCGGAAGATGGTTCAGAGCTTGAAAGAGATAGTCAATAACTGCACTGAGCAGGAGATCTATGCTATGCTTAAAGACTGTAACATGGACCCTAATGAAGCCGTTAATCGTCTCCTAGCTCAAGATCCTTTCCATGAAGTGAAGAGCAAACGAGAGAAGAAAAAGGAGAGTAAGGAGCCAACGGATTCTAGGTCTCGTGGTGCTAATATTTCAAATCATGGTGGTAGAGGTGGTGATCGTTATGCTGCACGTGGCGGATCAAACCACTTTAGCACAAATGAGTCTGGTTCTTTGCATGGTAGACCTGCGTACAGAAAGGAAAATGGAACACCTGCCTATGCAGGTTCTTCCTCTTCTGCAGCTGGCACAACTGGATATAACTTGAGCCGACGACCTCCATCCTACAGTGATCCAGTGGCAGCTGAAAATAAAATTATGTCAGTAGGTGATGACGGAATATCTTCATCCTCACAGCCTTCCTCTGGATATCAGTCTGCATGGGGAGGGGTGCCAGGTCAAGTTTCAATGGCTGACATTGTTAAGAAGGGTAGGCCGCAAGCCAAGGCACCCCCAACTTATAGTGTCAATCATCATGATACAGGGGCACCTCCTGCAGCAGGATACAATTCGAATTCATCGCAGACTGAGCCCTTGGCTGATGAGTGGCCCTTGATTGAGCATCCTCCAGGTGTTAGCATGTCCTCCATTTTAGGGGCACCTGCAAATTCTGAGCTCTATGCTAATTCATCTAGCGTGCCCTTGGATAAAGCTAATCAACATATAAAATCCCAACTAGATGAGGTTGAGACGGAAGATGATGGTTCTGTCGAGGTCGAGGCACTGAATACAAGCCATGATGGACCTACTTCTGTTTCCGGTAGAAATGTGCAAGAAGATAACTCGGCAACTGCATCTGCTTTTGATAATAGCTTGTATGAGGATATCAATTCCTACCATCCAGAGAGACATGACCTTGAGGATAATGAAGCTGAGCATGATGCTTCATCAATGGCTGCAAACTTGCAGCAGCTTGACTTACAGAAGGATGATAGGGGAGCACCACCTGAAGACGAGAACCCTCCCCCTGTAGTGATTCCAAATCACCTACAACTTCATACTCCAGACTGCTTGCACTTGAGCTTTGGAAGTTTCCGGTCCGGCCCTGAGCCTGATCTTTCGAGCTCCCATCCAGTGAAAAGTGACTTGGAAGAGACATCTGTAGCAGTAGATGTTTCTGCAGTCGGGCACTCAGATGCTAGAAATCCCGACTACTATGGAGATGAGCATCTCATAAATACCTCTGATGGAAGTTTGGTTCATAGAACTGGGGCCAGTGCTGGAGATTATGATTCTCCTTCAGTGTCACAACCAGAGGTCTTAAAACAGGAAACCCCTGAAGCTGGTCAGGGCATTCAATATACTTTTCCTTCTGCACCTGGATTTGCATATGACAGCTCCCAACAATTGAATATGGCATTTTCTCACCCACAGAGCTCACAAGTACAAAACAATGCTCCCTTTTCAAGTGTGATGGGATATACGAATTCGTTGCCTAGCACTCTATTGGCTTCAAATGCTCAGAATGTAAGGGAGGATCTTCCATACTCACCTTTCCCTGTGACACAATCTATGCCTACAAAGTATAGCAATGTGCCTTCTTCCATCAGCGGTCCCACCATGTCCATGCCAGAGGCTTTGAGAGCAGGTGGTATCTCTTCTGCTCAACCTACTCAACAGACCCTACCTGGTGCTGGTGTCGCGACTGGACCTGCTCTTCCACAACAGCTGTTGCACCCTTACTCTCAACCTAGTCTTCCTTTGGGACATTTTTCCAACATGATTGGTTATCCTTTCTTGCCTCAGAGCTACACATATATGCCATCAGCATTCCAGCAAGCTTTTGCTGGTAACAGCACATACCATCAGTCGCTGGCTGCAGTTCTCCCACAGTATAAAAATAGTGTTTCTGTCAGCAGTTTGCCCCAGTCTGCTAATATTCCCTCTGGTTATGGGTTTGGTAGTTCAAGTAACATTCCTGGAGGAAACTTTCCTCTGAATCCACCGTCGGCACCTACTGGTACAACAATTGGTTATGATGATGTTATAAGCTCTCAGTACAAGGACAGTAACCATTTGATGTCACTTCAGCAGAATGACAACTCAGGCATGTGGGTTCACGGACCTGGTTCCCGAGCAATGCCCGCTGTTCCAGCCAGCAATTATTACAGCTTCCAGGGGCAGAATCAACAGCATGCTGGTTTCCGGCAAACCCAGCAACCGTCGCAGCAATTTGCTGGAGCTCTAAACTACCCGAACTTCTATCATTCTCAGACAGGGATGTCCCTCGATCATCAACAGCAAAGTTCAAGGGACGCATCCCTGGGTGGCTCACAGGGACAACCTTCTAAGCAGTCTCAACAATTATGGCAAAACAGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

834

Amino Acids

88.8

Weight (kDa)

5.22

Isoelectric Point (pI)

62.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 18 - 77 9.3e-34 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2168
AarI CACCTGC 3 cut(s) 403, 790, 1757
AasI GACNNNNNNGTC 1 cut(s) 2401
Acc36I ACCTGC 6 cut(s) 376, 395, 403, 790, 1757, 1840
AccB1I GGYRCC 5 cut(s) 571, 628, 667, 778, 2131
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 364
AccII CGCG 1 cut(s) 1823
AciI CCGC 7 cut(s) 6, 15, 18, 318, 617, 1731, 2265
AclWI GGATC 4 cut(s) 182, 328, 467, 1665
AcsI RAATTY 3 cut(s) 694, 787, 1606
AcuI CTGAAG 4 cut(s) 1149, 1404, 1479, 2192
AcvI CACGTG 1 cut(s) 314
AfaI GTAC 4 cut(s) 374, 1566, 2143, 2180
AfiI CCNNNNNNNGG 6 cut(s) 73, 545, 680, 774, 1463, 1814
AgsI TTSAA 9 cut(s) 88, 276, 588, 1478, 1531, 1587, 1635, 2088, 2430
AjnI CCWGG 8 cut(s) 574, 748, 1498, 1807, 2098, 2245, 2295, 2442
AjuI GAANNNNNNNTTGG 2 cut(s) 1250, 1282
AloI GAACNNNNNNTCC 2 cut(s) 1362, 1394
Alw21I GWGCWC 5 cut(s) 798, 1123, 1247, 1559, 2365
Alw26I GTCTC 6 cut(s) 179, 261, 863, 1021, 1268, 2481
AlwI GGATC 4 cut(s) 182, 328, 467, 1665
AlwNI CAGNNNCTG 1 cut(s) 1993
Ama87I CYCGRG 1 cut(s) 2253
AoxI GGCC 4 cut(s) 614, 728, 1222, 1392
ApeKI GCWGC 9 cut(s) 308, 422, 482, 677, 1076, 1087, 1090, 1997, 2347
ApoI RAATTY 3 cut(s) 694, 787, 1606
Asp700I GAANNNNTTC 1 cut(s) 523
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 5 cut(s) 308, 521, 1157, 1538, 1662
AsuII TTCGAA 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 2253
AvaII GGWCC 7 cut(s) 63, 151, 923, 1218, 1733, 1829, 2243
BaeGI GKGCMC 4 cut(s) 670, 781, 822, 1311
BanI GGYRCC 5 cut(s) 571, 628, 667, 778, 2131
BanII GRGCYC 5 cut(s) 714, 798, 1247, 1559, 2365
BauI CACGAG 1 cut(s) 258
BbrPI CACGTG 1 cut(s) 314
BbsI GAAGAC 2 cut(s) 1137, 1868
Bbv12I GWGCWC 5 cut(s) 798, 1123, 1247, 1559, 2365
BbvI GCAGC 9 cut(s) 295, 434, 494, 689, 1063, 1099, 1102, 1984, 2359
BceAI ACGGC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 1594, 1628
BciT130I CCWGG 8 cut(s) 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
BciVI GTATCC 1 cut(s) 677
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 6 cut(s) 179, 261, 863, 1021, 1268, 2481
BfaI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1617, 1872
BfmI CTRYAG 7 cut(s) 420, 466, 675, 1149, 1281, 1299, 1998
BfuAI ACCTGC 6 cut(s) 376, 395, 403, 790, 1757, 1840
BfuI GTATCC 1 cut(s) 677
BglII AGATCT 1 cut(s) 120
BlpI GCTNAGC 1 cut(s) 1053
Bme1390I CCNGG 9 cut(s) 67, 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
Bme18I GGWCC 7 cut(s) 63, 151, 923, 1218, 1733, 1829, 2243
BmeT110I CYCGRG 1 cut(s) 2253
BmrFI CCNGG 9 cut(s) 67, 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
BmrI ACTGGG 2 cut(s) 1398, 2041
BmsI GCATC 6 cut(s) 751, 983, 1051, 1306, 1357, 2447
BmuI ACTGGG 2 cut(s) 1398, 2041
BpiI GAAGAC 2 cut(s) 1137, 1868
BpmI CTGGAG 5 cut(s) 732, 1167, 1422, 2121, 2379
Bpu10I CCTNAGC 1 cut(s) 1227
Bpu1102I GCTNAGC 1 cut(s) 1053
Bpu14I TTCGAA 1 cut(s) 692
BpuEI CTTGAG 4 cut(s) 168, 466, 1058, 1219
BpuMI CCSGG 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 1347
BsaI GGTCTC 1 cut(s) 261
BsaJI CCNNGG 6 cut(s) 624, 714, 822, 2296, 2442, 2443
BsaWI WCCGGW 2 cut(s) 938, 1215
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 545, 680, 774, 1463, 1814
Bse1I ACTGG 9 cut(s) 43, 439, 476, 1253, 1393, 1395, 1831, 2047, 2143
Bse3DI GCAATG 3 cut(s) 28, 1717, 2265
Bse8I GATNNNNATC 1 cut(s) 1347
BseBI CCWGG 8 cut(s) 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
BseDI CCNNGG 6 cut(s) 624, 714, 822, 2296, 2442, 2443
BseGI GGATG 8 cut(s) 461, 527, 742, 1018, 1114, 1249, 2406, 2438
BseJI GATNNNNATC 1 cut(s) 1347
BseLI CCNNNNNNNGG 6 cut(s) 73, 545, 680, 774, 1463, 1814
BseMI GCAATG 3 cut(s) 28, 1717, 2265
BseNI ACTGG 9 cut(s) 43, 439, 476, 1253, 1393, 1395, 1831, 2047, 2143
BseSI GKGCMC 4 cut(s) 670, 781, 822, 1311
BseXI GCAGC 9 cut(s) 295, 434, 494, 689, 1063, 1099, 1102, 1984, 2359
BseYI CCCAGC 2 cut(s) 59, 2334
BsgI GTGCAG 3 cut(s) 91, 294, 1479
Bsh1236I CGCG 1 cut(s) 1823
BshFI GGCC 4 cut(s) 616, 730, 1224, 1394
BshNI GGYRCC 5 cut(s) 571, 628, 667, 778, 2131
BsiHKAI GWGCWC 5 cut(s) 798, 1123, 1247, 1559, 2365
BsiHKCI CYCGRG 1 cut(s) 2253
BsiSI CCGG 5 cut(s) 67, 939, 1216, 1221, 2326
BslFI GGGAC 6 cut(s) 49, 1719, 1899, 2389, 2447, 2472
BslI CCNNNNNNNGG 6 cut(s) 73, 545, 680, 774, 1463, 1814
BsmAI GTCTC 6 cut(s) 179, 261, 863, 1021, 1268, 2481
BsmBI CGTCTC 2 cut(s) 179, 863
BsmFI GGGAC 6 cut(s) 49, 1719, 1899, 2389, 2447, 2472
BsmI GAATGC 2 cut(s) 1473, 1949
BsnI GGCC 4 cut(s) 616, 730, 1224, 1394
Bso31I GGTCTC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 2253
Bsp119I TTCGAA 1 cut(s) 692
Bsp143I GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1657, 2410
Bsp1720I GCTNAGC 1 cut(s) 1053
Bsp68I TCGCGA 1 cut(s) 1823
BspACI CCGC 7 cut(s) 6, 15, 18, 318, 617, 1731, 2265
BspANI GGCC 4 cut(s) 616, 730, 1224, 1394
BspFNI CGCG 1 cut(s) 1823
BspHI TCATGA 1 cut(s) 655
BspMAI CTGCAG 4 cut(s) 424, 679, 1303, 2002
BspMI ACCTGC 6 cut(s) 376, 395, 403, 790, 1757, 1840
BspPI GGATC 4 cut(s) 182, 328, 467, 1665
BspQI GCTCTTC 2 cut(s) 200, 1842
BspT104I TTCGAA 1 cut(s) 692
BspT107I GGYRCC 5 cut(s) 571, 628, 667, 778, 2131
BspTNI GGTCTC 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 3 cut(s) 28, 1717, 2265
BsrI ACTGG 9 cut(s) 43, 439, 476, 1253, 1393, 1395, 1831, 2047, 2143
BssECI CCNNGG 6 cut(s) 624, 714, 822, 2296, 2442, 2443
BssMI GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1657, 2410
BssSI CACGAG 1 cut(s) 258
BssT1I CCWWGG 3 cut(s) 624, 714, 822
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 8 cut(s) 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
Bst4CI ACNGT 6 cut(s) 143, 470, 2013, 2127, 2189, 2343
Bst6I CTCTTC 5 cut(s) 200, 421, 1266, 1783, 1842
BstAPI GCANNNNNTGC 1 cut(s) 674
BstBAI YACGTR 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 692
BstC8I GCNNGC 9 cut(s) 427, 621, 818, 1193, 1959, 1995, 2265, 2278, 2316
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 8 cut(s) 461, 527, 742, 1018, 1114, 1249, 2406, 2438
BstFNI CGCG 1 cut(s) 1823
BstKTI GATC 8 cut(s) 123, 190, 301, 323, 475, 1237, 1660, 2413
BstMAI GTCTC 6 cut(s) 179, 261, 863, 1021, 1268, 2481
BstMBI GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1657, 2410
BstNI CCWGG 8 cut(s) 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
BstNSI RCATGY 3 cut(s) 763, 2233, 2318
BstSCI CCNGG 9 cut(s) 65, 574, 748, 1498, 1807, 2098, 2245, 2295, 2442
BstSFI CTRYAG 7 cut(s) 420, 466, 675, 1149, 1281, 1299, 1998
BstSLI GKGCMC 4 cut(s) 670, 781, 822, 1311
BstUI CGCG 1 cut(s) 1823
BstV1I GCAGC 9 cut(s) 295, 434, 494, 689, 1063, 1099, 1102, 1984, 2359
BstV2I GAAGAC 2 cut(s) 1137, 1868
BstX2I RGATCY 3 cut(s) 120, 187, 1657
BstYI RGATCY 3 cut(s) 120, 187, 1657
BsuI GTATCC 1 cut(s) 677
BsuRI GGCC 4 cut(s) 616, 730, 1224, 1394
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 8 cut(s) 461, 527, 742, 1018, 1114, 1249, 2406, 2438
BtsIMutI CAGTG 7 cut(s) 108, 475, 483, 902, 1260, 1402, 1428
BtuMI TCGCGA 1 cut(s) 1823
BveI ACCTGC 6 cut(s) 376, 395, 403, 790, 1757, 1840
Cac8I GCNNGC 9 cut(s) 427, 621, 818, 1193, 1959, 1995, 2265, 2278, 2316
CaiI CAGNNNCTG 1 cut(s) 1993
CciI TCATGA 1 cut(s) 655
CpoI CGGWCCG 1 cut(s) 1218
CseI GACGC 1 cut(s) 2444
CsiI ACCWGGT 2 cut(s) 1807, 2245
Csp6I GTAC 4 cut(s) 373, 1565, 2142, 2179
CspI CGGWCCG 1 cut(s) 1218
CviQI GTAC 4 cut(s) 373, 1565, 2142, 2179
DpnI GATC 8 cut(s) 122, 189, 300, 322, 474, 1236, 1659, 2412
DpnII GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1657, 2410
DrdI GACNNNNNNGTC 1 cut(s) 2401
DseDI GACNNNNNNGTC 1 cut(s) 2401
Eam1104I CTCTTC 5 cut(s) 200, 421, 1266, 1783, 1842
EarI CTCTTC 5 cut(s) 200, 421, 1266, 1783, 1842
EciI GGCGGA 1 cut(s) 333
Ecl136II GAGCTC 4 cut(s) 796, 1245, 1557, 2363
Eco130I CCWWGG 3 cut(s) 624, 714, 822
Eco24I GRGCYC 5 cut(s) 714, 798, 1247, 1559, 2365
Eco31I GGTCTC 1 cut(s) 261
Eco32I GATATC 2 cut(s) 551, 1006
Eco47I GGWCC 7 cut(s) 63, 151, 923, 1218, 1733, 1829, 2243
Eco53kI GAGCTC 4 cut(s) 796, 1245, 1557, 2363
Eco57I CTGAAG 4 cut(s) 1149, 1404, 1479, 2192
Eco72I CACGTG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 2253
EcoICRI GAGCTC 4 cut(s) 796, 1245, 1557, 2363
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 63
EcoRI GAATTC 2 cut(s) 694, 1606
EcoRII CCWGG 8 cut(s) 574, 748, 1498, 1807, 2098, 2245, 2295, 2442
EcoRV GATATC 2 cut(s) 551, 1006
EcoT14I CCWWGG 3 cut(s) 624, 714, 822
EcoT38I GRGCYC 5 cut(s) 714, 798, 1247, 1559, 2365
ErhI CCWWGG 3 cut(s) 624, 714, 822
Esp3I CGTCTC 2 cut(s) 179, 863
FaqI GGGAC 6 cut(s) 49, 1719, 1899, 2389, 2447, 2472
FauI CCCGC 1 cut(s) 2272
FauNDI CATATG 1 cut(s) 1510
FblI GTMKAC 1 cut(s) 364
FokI GGATG 8 cut(s) 448, 514, 729, 1005, 1121, 1236, 2413, 2425
FriOI GRGCYC 5 cut(s) 714, 798, 1247, 1559, 2365
FspBI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1617, 1872
GsaI CCCAGC 2 cut(s) 63, 2338
GsuI CTGGAG 5 cut(s) 732, 1167, 1422, 2121, 2379
HaeIII GGCC 4 cut(s) 616, 730, 1224, 1394
HapII CCGG 5 cut(s) 67, 939, 1216, 1221, 2326
HgaI GACGC 1 cut(s) 2444
HindIII AAGCTT 1 cut(s) 1959
HinfI GANTC 6 cut(s) 248, 344, 1156, 1412, 2119, 2305
HpaII CCGG 5 cut(s) 67, 939, 1216, 1221, 2326
HphI GGTGA 5 cut(s) 308, 521, 1157, 1538, 1662
Hpy166II GTNNAC 2 cut(s) 365, 2239
Hpy188I TCNGA 8 cut(s) 81, 793, 1315, 1366, 1644, 1926, 2118, 2394
Hpy188III TCNNGA 9 cut(s) 48, 185, 546, 656, 1022, 1184, 1328, 1822, 2253
Hpy8I GTNNAC 2 cut(s) 365, 2239
Hpy99I CGWCG 3 cut(s) 456, 2131, 2347
HpyAV CCTTC 8 cut(s) 22, 549, 601, 1099, 1428, 1500, 1728, 2475
HpyCH4III ACNGT 6 cut(s) 143, 470, 2013, 2127, 2189, 2343
HpyCH4IV ACGT 1 cut(s) 313
HpySE526I ACGT 1 cut(s) 313
KflI GGGWCCC 1 cut(s) 63
Kzo9I GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1657, 2410
LguI GCTCTTC 2 cut(s) 200, 1842
LmnI GCTCC 6 cut(s) 238, 1118, 1250, 1523, 1582, 2360
Lsp1109I GCAGC 9 cut(s) 295, 434, 494, 689, 1063, 1099, 1102, 1984, 2359
LweI GCATC 6 cut(s) 751, 983, 1051, 1306, 1357, 2447
MabI ACCWGGT 2 cut(s) 1807, 2245
MaeI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1617, 1872
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 8 cut(s) 143, 1262, 1425, 1681, 1970, 2090, 2189, 2202
MalI GATC 8 cut(s) 122, 189, 300, 322, 474, 1236, 1659, 2412
MbiI CCGCTC 1 cut(s) 6
MboI GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1657, 2410
MfeI CAATTG 2 cut(s) 1526, 2148
MflI RGATCY 3 cut(s) 120, 187, 1657
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 1920
MroXI GAANNNNTTC 1 cut(s) 523
MseI TTAA 4 cut(s) 135, 168, 603, 1442
MslI CAYNNNNRTG 3 cut(s) 1314, 1745, 1937
MspA1I CMGCKG 6 cut(s) 59, 425, 485, 1731, 1849, 2267
MspI CCGG 5 cut(s) 67, 939, 1216, 1221, 2326
MspR9I CCNGG 9 cut(s) 67, 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
MunI CAATTG 2 cut(s) 1526, 2148
Mva1269I GAATGC 2 cut(s) 1473, 1949
MvaI CCWGG 8 cut(s) 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
MvnI CGCG 1 cut(s) 1823
NciI CCSGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 1510
NdeII GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1657, 2410
NmeAIII GCCGAG 1 cut(s) 944
NmuCI GTSAC 4 cut(s) 1262, 1425, 1681, 2202
NruI TCGCGA 1 cut(s) 1823
NspI RCATGY 3 cut(s) 763, 2233, 2318
NspV TTCGAA 1 cut(s) 692
PaeI GCATGC 1 cut(s) 2318
PagI TCATGA 1 cut(s) 655
PaqCI CACCTGC 3 cut(s) 403, 790, 1757
PasI CCCWGGG 1 cut(s) 2443
PciSI GCTCTTC 2 cut(s) 200, 1842
PctI GAATGC 2 cut(s) 1473, 1949
PdmI GAANNNNTTC 1 cut(s) 523
PfeI GAWTC 5 cut(s) 248, 1156, 1412, 2119, 2305
PfoI TCCNGGA 2 cut(s) 65, 2098
PleI GAGTC 1 cut(s) 352
PmaCI CACGTG 1 cut(s) 314
PmlI CACGTG 1 cut(s) 314
PpsI GAGTC 1 cut(s) 352
Ppu21I YACGTR 1 cut(s) 314
PpuMI RGGWCCY 1 cut(s) 63
PsiI TTATAA 1 cut(s) 2168
Psp124BI GAGCTC 4 cut(s) 798, 1247, 1559, 2365
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 8 cut(s) 574, 748, 1498, 1807, 2098, 2245, 2295, 2442
PspCI CACGTG 1 cut(s) 314
PspFI CCCAGC 2 cut(s) 59, 2334
PspGI CCWGG 8 cut(s) 574, 748, 1498, 1807, 2098, 2245, 2295, 2442
PspPPI RGGWCCY 1 cut(s) 63
PstI CTGCAG 4 cut(s) 424, 679, 1303, 2002
PstNI CAGNNNCTG 1 cut(s) 1993
PsuI RGATCY 3 cut(s) 120, 187, 1657
PvuII CAGCTG 4 cut(s) 59, 425, 485, 1849
RruI TCGCGA 1 cut(s) 1823
RsaI GTAC 4 cut(s) 374, 1566, 2143, 2180
RsaNI GTAC 4 cut(s) 373, 1565, 2142, 2179
RseI CAYNNNNRTG 3 cut(s) 1314, 1745, 1937
Rsr2I CGGWCCG 1 cut(s) 1218
RsrII CGGWCCG 1 cut(s) 1218
SacI GAGCTC 4 cut(s) 798, 1247, 1559, 2365
SapI GCTCTTC 2 cut(s) 200, 1842
SaqAI TTAA 4 cut(s) 135, 168, 603, 1442
Sau3AI GATC 8 cut(s) 120, 187, 298, 320, 472, 1234, 1657, 2410
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 9 cut(s) 67, 576, 750, 1500, 1809, 2100, 2247, 2297, 2444
SexAI ACCWGGT 2 cut(s) 1807, 2245
SfaNI GCATC 6 cut(s) 751, 983, 1051, 1306, 1357, 2447
SfcI CTRYAG 7 cut(s) 420, 466, 675, 1149, 1281, 1299, 1998
SfuI TTCGAA 1 cut(s) 692
SinI GGWCC 7 cut(s) 63, 151, 923, 1218, 1733, 1829, 2243
SmiMI CAYNNNNRTG 3 cut(s) 1314, 1745, 1937
SmlI CTYRAG 4 cut(s) 183, 445, 1037, 1198
SmoI CTYRAG 4 cut(s) 183, 445, 1037, 1198
SphI GCATGC 1 cut(s) 2318
SsiI CCGC 7 cut(s) 6, 15, 18, 318, 617, 1731, 2265
SspI AATATT 2 cut(s) 271, 2059
SspMI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1617, 1872
SstI GAGCTC 4 cut(s) 798, 1247, 1559, 2365
StyD4I CCNGG 9 cut(s) 65, 574, 748, 1498, 1807, 2098, 2245, 2295, 2442
StyI CCWWGG 3 cut(s) 624, 714, 822
TaaI ACNGT 6 cut(s) 143, 470, 2013, 2127, 2189, 2343
TaiI ACGT 1 cut(s) 316
TaqI TCGA 5 cut(s) 692, 891, 897, 1241, 2409
TatI WGTACW 2 cut(s) 1564, 2178
TauI GCSGC 3 cut(s) 18, 21, 619
TfiI GAWTC 5 cut(s) 248, 1156, 1412, 2119, 2305
Tru1I TTAA 4 cut(s) 135, 168, 603, 1442
Tru9I TTAA 4 cut(s) 135, 168, 603, 1442
TscAI CASTG 7 cut(s) 115, 475, 483, 909, 1260, 1402, 1428
TseFI GTSAC 4 cut(s) 1262, 1425, 1681, 2202
TseI GCWGC 9 cut(s) 308, 422, 482, 677, 1076, 1087, 1090, 1997, 2347
Tsp45I GTSAC 4 cut(s) 1262, 1425, 1681, 2202
TspDTI ATGAA 9 cut(s) 174, 213, 516, 687, 798, 1056, 1062, 1166, 1370
TspGWI ACGGA 4 cut(s) 260, 531, 887, 2256
TspRI CASTG 7 cut(s) 115, 475, 483, 909, 1260, 1402, 1428
VpaK11BI GGWCC 7 cut(s) 63, 151, 923, 1218, 1733, 1829, 2243
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 3 cut(s) 694, 787, 1606
XceI RCATGY 3 cut(s) 763, 2233, 2318
XmiI GTMKAC 1 cut(s) 364
XmnI GAANNNNTTC 1 cut(s) 523
XspI CTAG 7 cut(s) 179, 252, 813, 857, 1320, 1617, 1872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.