FvH4_3g07290

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018812
Physical Location & Seq
Forward (+)
4333205 .. 4334254
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07290.t1

Sequence Viewer

Length: 1050 bp
ATGCCTCTAAATTGCTCAAACTGCAAAACATATCTGCAGGCACTACCTACACGTTCTCAGAATGTATGCTGCTCCAGATGCAAGACCCTCGTGCGCTTTGCATACTCAATGTGTCCACCTCCTCCTCCTCCCCGGTCATACTCCTCCTCTTTCTCCTACAACAACATCGAGCTCCCTCCTCCCCTCTCATATGCCTTCTCCTTTTTCTCCTACAACAAACGCCCTCCTCCGCCTTCTCGGTTCCCCCACCAACGCAAGCGAGCCGTGATATGTGGCATAAATTACAAGGGAATGGGGAAGAATACGGAGCTCGATGGCTGCACTAACGATGCCATTTCAATGCAGGCCTTGCTGGATAGTGAGTTCAAATTCCCTGCGTCTTCCATTCGCATGCTCACTGAAATGAAAGGTCATCAGGCCCCTACCAAGACAAACATAACGTTAGCACTACGTTGGCTTGTGCAAGATTGTCAAGCTGGAGATTCGTTGGTGTTTTATTTTTCCGGTCATGGTGATCAGAAGCCGAACAACACAGGAGACGAGGAGGACGAACTAGACGAGACACTCTTACCCTTAGATTACGGGACTAAGGGAGTGATCGTTGATGACGAGATCAATGACATTATTGTCCGACCTATTCCGGCTGGTGTCAAGCTGCATGCTATTGTGGATGCTTGCCACAGTGGTACTGTTCTTGACTTGCCGTTTCTTTGTAAAATAGACAAAAGTGGACGGAGGTGGTTCTGGAAGGAACATAGAGGACCTAAGAAAGGTGGTGTTTGGAAAGGAACAAAAGGTGGAGAGGTCATTTCCTTTAGCGGATGTGATGATCATCAGAATTCTGTGGAGAAAGGAATCCCACGAGGTGCAATGACTTGGGCTTTCATCGATGCCATTGATCGAGGGTGTGGAAATACATATGGAAACTTGTTGCTTGCAATGCGATCTACCGTGGTTAAGGATTGTATTGATGCAGCCTCACCGATACAAGAACCACAGCTAACTTCAAGTGAAGTGTTTGATGTATTCACCAGGCCTTTCTCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010941 GO:0043067 GO:0043069 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C14 zf-LSD1

Protein Analysis

350

Amino Acids

38.72

Weight (kDa)

7.88

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 85 - 342 4.6e-33 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018812)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g07290
rosa_chinensis RchiOBHm_Chr5g0011711
rosa_laevigata RLG00000031857
rosa_multiflora Rmu_co8248297.1_g000001 Rmu_sc0007176.1_g000004
rosa_roxburghii Rroxscaffold_1G00064810
rosa_rugosa Rorug04G0458600
rosa_samantha Rh5DG085800
rosa_wichuraiana Rw5G007870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 626
AciI CCGC 2 cut(s) 230, 819
AclI AACGTT 1 cut(s) 440
AcsI RAATTY 2 cut(s) 368, 838
AdeI CACNNNGTG 1 cut(s) 866
AfaI GTAC 1 cut(s) 688
AfiI CCNNNNNNNGG 1 cut(s) 770
AflIII ACRYGT 1 cut(s) 50
AgsI TTSAA 3 cut(s) 339, 367, 1008
AjnI CCWGG 1 cut(s) 1031
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
AluBI AGCT 5 cut(s) 172, 310, 476, 655, 1000
AluI AGCT 5 cut(s) 172, 310, 476, 655, 1000
Alw21I GWGCWC 2 cut(s) 174, 312
Alw26I GTCTC 2 cut(s) 531, 554
AoxI GGCC 3 cut(s) 345, 417, 1034
ApeKI GCWGC 4 cut(s) 69, 318, 655, 974
ApoI RAATTY 2 cut(s) 368, 838
AspLEI GCGC 1 cut(s) 96
AspS9I GGNCC 2 cut(s) 418, 761
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 3 cut(s) 524, 972, 1021
AvaII GGWCC 1 cut(s) 761
BanII GRGCYC 2 cut(s) 174, 312
BauI CACGAG 2 cut(s) 89, 861
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 174, 312
BbvI GCAGC 4 cut(s) 56, 305, 642, 986
BccI CCATC 1 cut(s) 308
BceAI ACGGC 2 cut(s) 248, 688
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 1 cut(s) 1033
BclI TGATCA 2 cut(s) 514, 829
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 531, 554
BfaI CTAG 1 cut(s) 554
BfmI CTRYAG 2 cut(s) 35, 1046
BisI GCNGC 4 cut(s) 70, 319, 656, 975
BlsI GCNGC 4 cut(s) 71, 320, 657, 976
Bme1390I CCNGG 2 cut(s) 133, 1033
Bme18I GGWCC 1 cut(s) 761
BmgT120I GGNCC 2 cut(s) 418, 761
BmiI GGNNCC 2 cut(s) 242, 420
BmrFI CCNGG 2 cut(s) 133, 1033
BmsI GCATC 5 cut(s) 68, 319, 661, 880, 961
BpiI GAAGAC 1 cut(s) 372
BpmI CTGGAG 2 cut(s) 58, 498
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 888
BsaBI GATNNNNATC 1 cut(s) 831
BsaJI CCNNGG 2 cut(s) 131, 951
BsaWI WCCGGW 1 cut(s) 503
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse3DI GCAATG 2 cut(s) 876, 945
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 1 cut(s) 1033
BseCI ATCGAT 1 cut(s) 888
BseDI CCNNGG 2 cut(s) 131, 951
BseGI GGATG 2 cut(s) 676, 827
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMI GCAATG 2 cut(s) 876, 945
BseMII CTCAG 1 cut(s) 71
BseRI GAGGAG 8 cut(s) 111, 114, 117, 133, 136, 168, 216, 557
BseXI GCAGC 4 cut(s) 56, 305, 642, 986
BsgI GTGCAG 1 cut(s) 304
BshFI GGCC 3 cut(s) 347, 419, 1036
BshVI ATCGAT 1 cut(s) 888
BsiHKAI GWGCWC 2 cut(s) 174, 312
BsiSI CCGG 3 cut(s) 133, 504, 641
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 1 cut(s) 770
BsmAI GTCTC 2 cut(s) 531, 554
BsmBI CGTCTC 1 cut(s) 531
BsmFI GGGAC 1 cut(s) 598
BsnI GGCC 3 cut(s) 347, 419, 1036
Bsp1286I GDGCHC 2 cut(s) 174, 312
Bsp143I GATC 6 cut(s) 514, 597, 612, 829, 898, 944
BspACI CCGC 2 cut(s) 230, 819
BspANI GGCC 3 cut(s) 347, 419, 1036
BspCNI CTCAG 1 cut(s) 70
BspDI ATCGAT 1 cut(s) 888
BspLI GGNNCC 2 cut(s) 242, 420
BspMAI CTGCAG 1 cut(s) 39
BsrDI GCAATG 2 cut(s) 876, 945
BssECI CCNNGG 2 cut(s) 131, 951
BssMI GATC 6 cut(s) 514, 597, 612, 829, 898, 944
BssSI CACGAG 2 cut(s) 89, 861
Bst2BI CACGAG 2 cut(s) 89, 861
Bst2UI CCWGG 1 cut(s) 1033
Bst4CI ACNGT 3 cut(s) 683, 691, 952
BstAPI GCANNNNNTGC 1 cut(s) 349
BstC8I GCNNGC 8 cut(s) 39, 257, 261, 345, 392, 660, 676, 936
BstDEI CTNAG 4 cut(s) 57, 574, 588, 765
BstDSI CCRYGG 1 cut(s) 951
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 2 cut(s) 676, 827
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 6 cut(s) 517, 600, 615, 832, 901, 947
BstMAI GTCTC 2 cut(s) 531, 554
BstMBI GATC 6 cut(s) 514, 597, 612, 829, 898, 944
BstMWI GCNNNNNNNGC 4 cut(s) 21, 78, 349, 940
BstNI CCWGG 1 cut(s) 1033
BstNSI RCATGY 2 cut(s) 394, 662
BstSCI CCNGG 2 cut(s) 131, 1031
BstSFI CTRYAG 2 cut(s) 35, 1046
BstV1I GCAGC 4 cut(s) 56, 305, 642, 986
BstV2I GAAGAC 1 cut(s) 372
Bsu15I ATCGAT 1 cut(s) 888
BsuRI GGCC 3 cut(s) 347, 419, 1036
BsuTUI ATCGAT 1 cut(s) 888
BtgI CCRYGG 1 cut(s) 951
BtsCI GGATG 2 cut(s) 676, 827
BtsIMutI CAGTG 2 cut(s) 396, 688
Cac8I GCNNGC 8 cut(s) 39, 257, 261, 345, 392, 660, 676, 936
CfoI GCGC 1 cut(s) 96
Cfr13I GGNCC 2 cut(s) 418, 761
ClaI ATCGAT 1 cut(s) 888
CseI GACGC 1 cut(s) 366
Csp6I GTAC 1 cut(s) 687
CviAII CATG 3 cut(s) 391, 509, 659
CviQI GTAC 1 cut(s) 687
DdeI CTNAG 4 cut(s) 57, 574, 588, 765
DpnI GATC 6 cut(s) 516, 599, 614, 831, 900, 946
DpnII GATC 6 cut(s) 514, 597, 612, 829, 898, 944
DraIII CACNNNGTG 1 cut(s) 866
DrdI GACNNNNNNGTC 1 cut(s) 626
DseDI GACNNNNNNGTC 1 cut(s) 626
EciI GGCGGA 1 cut(s) 219
Ecl136II GAGCTC 2 cut(s) 172, 310
Eco147I AGGCCT 2 cut(s) 347, 1036
Eco24I GRGCYC 2 cut(s) 174, 312
Eco47I GGWCC 1 cut(s) 761
Eco53kI GAGCTC 2 cut(s) 172, 310
EcoICRI GAGCTC 2 cut(s) 172, 310
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoO109I RGGNCCY 2 cut(s) 418, 761
EcoRI GAATTC 1 cut(s) 838
EcoRII CCWGG 1 cut(s) 1031
EcoT38I GRGCYC 2 cut(s) 174, 312
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 3 cut(s) 394, 512, 662
FaqI GGGAC 1 cut(s) 598
FatI CATG 3 cut(s) 390, 508, 658
FauNDI CATATG 2 cut(s) 190, 919
FbaI TGATCA 2 cut(s) 514, 829
Fnu4HI GCNGC 4 cut(s) 70, 319, 656, 975
FokI GGATG 2 cut(s) 683, 834
FriOI GRGCYC 2 cut(s) 174, 312
Fsp4HI GCNGC 4 cut(s) 70, 319, 656, 975
FspBI CTAG 1 cut(s) 554
GlaI GCGC 1 cut(s) 95
GluI GCNGC 4 cut(s) 70, 319, 656, 975
GsuI CTGGAG 2 cut(s) 58, 498
HaeIII GGCC 3 cut(s) 347, 419, 1036
HapII CCGG 3 cut(s) 133, 504, 641
HgaI GACGC 1 cut(s) 366
HhaI GCGC 1 cut(s) 96
Hin1II CATG 3 cut(s) 394, 512, 662
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HinfI GANTC 2 cut(s) 482, 855
HpaII CCGG 3 cut(s) 133, 504, 641
HphI GGTGA 3 cut(s) 524, 972, 1021
Hpy166II GTNNAC 2 cut(s) 116, 731
Hpy188I TCNGA 4 cut(s) 60, 519, 632, 837
Hpy188III TCNNGA 3 cut(s) 75, 695, 745
Hpy8I GTNNAC 2 cut(s) 116, 731
HpyAV CCTTC 3 cut(s) 205, 243, 742
HpyCH4III ACNGT 3 cut(s) 683, 691, 952
HpyCH4IV ACGT 3 cut(s) 52, 440, 451
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 78, 349, 940
HpyF3I CTNAG 4 cut(s) 57, 574, 588, 765
HpySE526I ACGT 3 cut(s) 52, 440, 451
Hsp92II CATG 3 cut(s) 394, 512, 662
HspAI GCGC 1 cut(s) 94
Ksp22I TGATCA 2 cut(s) 514, 829
Kzo9I GATC 6 cut(s) 514, 597, 612, 829, 898, 944
LmnI GCTCC 3 cut(s) 77, 177, 307
Lsp1109I GCAGC 4 cut(s) 56, 305, 642, 986
LweI GCATC 5 cut(s) 68, 319, 661, 880, 961
MaeI CTAG 1 cut(s) 554
MaeII ACGT 3 cut(s) 52, 440, 451
MalI GATC 6 cut(s) 516, 599, 614, 831, 900, 946
MboI GATC 6 cut(s) 514, 597, 612, 829, 898, 944
MboII GAAGA 2 cut(s) 310, 372
MhlI GDGCHC 2 cut(s) 174, 312
MluCI AATT 4 cut(s) 10, 280, 368, 838
MmeI TCCRAC 1 cut(s) 655
MseI TTAA 1 cut(s) 957
MslI CAYNNNNRTG 3 cut(s) 338, 389, 401
MspI CCGG 3 cut(s) 133, 504, 641
MspR9I CCNGG 2 cut(s) 133, 1033
MvaI CCWGG 1 cut(s) 1033
MwoI GCNNNNNNNGC 4 cut(s) 21, 78, 349, 940
NciI CCSGG 1 cut(s) 133
NdeI CATATG 2 cut(s) 190, 919
NdeII GATC 6 cut(s) 514, 597, 612, 829, 898, 944
NlaIII CATG 3 cut(s) 394, 512, 662
NlaIV GGNNCC 2 cut(s) 242, 420
NspI RCATGY 2 cut(s) 394, 662
PaeI GCATGC 2 cut(s) 394, 662
PceI AGGCCT 2 cut(s) 347, 1036
PcsI WCGNNNNNNNCGW 3 cut(s) 546, 555, 606
PfeI GAWTC 2 cut(s) 482, 855
PkrI GCNGC 4 cut(s) 71, 320, 657, 976
PpuMI RGGWCCY 1 cut(s) 761
Psp124BI GAGCTC 2 cut(s) 174, 312
Psp1406I AACGTT 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 1031
PspGI CCWGG 1 cut(s) 1031
PspN4I GGNNCC 2 cut(s) 242, 420
PspPI GGNCC 2 cut(s) 418, 761
PspPPI RGGWCCY 1 cut(s) 761
PstI CTGCAG 1 cut(s) 39
RsaI GTAC 1 cut(s) 688
RsaNI GTAC 1 cut(s) 687
RseI CAYNNNNRTG 3 cut(s) 338, 389, 401
SacI GAGCTC 2 cut(s) 174, 312
SaqAI TTAA 1 cut(s) 957
SatI GCNGC 4 cut(s) 70, 319, 656, 975
Sau3AI GATC 6 cut(s) 514, 597, 612, 829, 898, 944
Sau96I GGNCC 2 cut(s) 418, 761
ScrFI CCNGG 2 cut(s) 133, 1033
SduI GDGCHC 2 cut(s) 174, 312
SfaNI GCATC 5 cut(s) 68, 319, 661, 880, 961
SfcI CTRYAG 2 cut(s) 35, 1046
SinI GGWCC 1 cut(s) 761
SmiMI CAYNNNNRTG 3 cut(s) 338, 389, 401
SphI GCATGC 2 cut(s) 394, 662
Sse9I AATT 4 cut(s) 10, 280, 368, 838
SseBI AGGCCT 2 cut(s) 347, 1036
SsiI CCGC 2 cut(s) 230, 819
SspMI CTAG 1 cut(s) 554
SstI GAGCTC 2 cut(s) 174, 312
StuI AGGCCT 2 cut(s) 347, 1036
StyD4I CCNGG 2 cut(s) 131, 1031
TaaI ACNGT 3 cut(s) 683, 691, 952
TaiI ACGT 3 cut(s) 55, 443, 454
TaqI TCGA 4 cut(s) 168, 312, 888, 901
TasI AATT 4 cut(s) 10, 280, 368, 838
TfiI GAWTC 2 cut(s) 482, 855
Tru1I TTAA 1 cut(s) 957
Tru9I TTAA 1 cut(s) 957
TscAI CASTG 2 cut(s) 403, 688
TseI GCWGC 4 cut(s) 69, 318, 655, 974
TspDTI ATGAA 2 cut(s) 419, 874
TspGWI ACGGA 2 cut(s) 320, 748
TspRI CASTG 2 cut(s) 403, 688
VpaK11BI GGWCC 1 cut(s) 761
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 2 cut(s) 368, 838
XceI RCATGY 2 cut(s) 394, 662
XspI CTAG 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.