Rorug04G0458600

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004881
Physical Location & Seq
Reverse (-)
61132605 .. 61140467
7863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0458600.1

Sequence Viewer

Length: 3090 bp
ATGTTTGGTGATGTGGATCACCTTCATGATTTGTTTCAGTCCACTTGGAGATGCTGCTCTTTCTTACGTGGATGGATTGGCAGTTTAATATTGAAGCATTGCACGGATGTGACTGGCTTACAATCAAGAAACTATCTTTCTAAACTGGTTAAAGAAAGGGAGCATCAACTTTCAACCTTCATGTCTCAAACGGCGTATATAGATCTTAGTTCATCTGACAGTGATTTAGAAGAGATAGAGCCTGTTGGTAGAAGAACTCTTCCACCTTCGTTTTCTGCATCTGCTTCTGCTTCTGCTTCTGGTTCAAATTCAAGGAGTAAAGACTATGCTGACAAGTCTAGAAAGGTGCCTTCTCCTAGAACAGCACATGCTTCTAATGGAATATCACCTAATTATAATCACCACAGACAAGTGTTAGATAAGTTTCATCCCAGTTCAAGTGGTGACATAAGAGCATCAAATCAACAGGTTGCCCGAGCAGATAGTTCTACGTATTTCTCTCAAAATGGAAATGCCCTAAAGAGGACCCTTCCATTATCTATGCAGGCTCTAAACCCTGCAGTAGATAACAGGGCCCACAATCAGTTCCGTGATACCACCAACAAGGGTTTTATGAGAGACCATTCAATCAGGGGGAATGATGAACATATGTATGATAGAATGCCTCCATTTGTAAAGTCATCGTCTAACTCCCAATTTCCTAGTTCAAGTGAGCCTCAATACCTTCCGGGAATAGGTGAAGAAAGGGTTGCTGAAAGTGACGAGAGGCTGATATATCAAGCGGCACTAGAGGAACTTAATCAACCAAAACTTGAAGCTACTTTACCTGATGGTCTTTTGTCGGTCTCTCTTCTACGACATCAGAAAATTGCTTTGGCATGGATGCTTCAGAAGGAAACCAGAAGCTTGCATTGTCTAGGTGGAATCTTAGCTGATGATCAGGGCCTTGGTAAGACAATCTCAATGATTGCACTTATACAAATGCAGAGGTCTTTGCAATCGAAGTCAAAACCTGAACACTTGGACAATAAAACTGAAGCTTTGAATTTGGATGATGATGAGGACCATGGTGGTTGCGGTTTGGATAAAGTCAACAAGACTGAAGAATCTGATTTTAAATCAACTCCAGAAGCTAGTACCTCTGCACAGCCATTCAAAAAGAAGAGGCCAGCAGCTGGTACATTGGTGGTTTGTCCAGCAAGTGTTCTTCGACAGTGGGCTAGGGAGCTGGATGAGAAGGTTGCAGAGGAAGCAAAATTGTCTGTACTTGTTTATCATGGAGGCAGCAGAACAAGGAATCCCGAAGAACTTGCTGGCTATGATGTGGTTCTCACTACATATGCTATTGTAACTAATGAAGTCCCAAAACAACCTTTAGTTGATGAGGATGAGGGGGATGAAAAAAATGTGGAAAAGTATGGATTATCTTCTGATTTTTCTGTCAACAAGAAGAGGAAAAAGGCTTCTATTGTTAGTAAGAAGGGAAAGAAAGGTAGGAAAGGATTTGACACTTCCCCTTTTGACTGTGGTTCTGGGCCACTTGCAAGGGTCGGTTGGTTTAGGGTGATACTAGATGAAGCGCAAACCATTAAGAATCACAGAACTCAAGTGGCTAGGGCCTGCTGTAGCCTTCGAGCCAAAAGAAGATGGTGTCTATCTGGTACACCAATACAAAATGCAATTGATGATTTATACAGTTACTTCAGATTTCTGAAATATGATCCTTATGCTGTGTATAAATCATTTTACACTACCATTAAGGTTCCAATATCCAGAAATTCACTCCAAGGTTACAAGAAGCTCCAAGCTGTTCTGAGAGCTATTATGCTGCGCCGGACAAAAGGAACAATGATTGATGGGCAACCTATAATTAATTTACCACCAAAAACAATTAACCTGAGTAAAGTGGAGTTCTCGTCTGAGGAGAGGGCCTTCTATACCAAATTAGAAGCTGATTCACGCTCTCAATTCAAGGCATATGCTGCTGCTGGCACAGTAAATCAAAACTATGCAAATATTCTTTTGATGCTTTTGCGCCTTCGGCAGGCTTGTGATCACCCTCTACTTGTTAAAGGATATGACACTGACTGCGTTGGGAAAGATTCTGTGGCTATGGCAAGTACACTTTCTAGAGAGATGCTTATCGATCTATTGAATGCCCTGGAAAGTTCCGAGGCCATTTGTCGTGTATGCAATGATGTACTTGAGAACCCTGTTGTTACTATGTGTGGCCATGTTTTCTGCCATCAGTGTGTGTCAGAAAATATGACTGGTGATGACAGTATGTGCCCTGCAATTGAATGCAAAAAACAAGTCAGTCCTGATGTTGTTTTCTCCGAATCCACTCTAATAAGTTGCCTCTCTAAGGATCTTGATGGTGGTTCAACGAATTCTCAGTTGATTGAGAAACCAGTAGTGGTACAGAATGAGTACACTTCATCTAAAGTCAGAGCTGTTATTGAGATAATACAGTCACACTGCAGGTTAAACAGCCCAAATTTGGAGCAATATAATTCTACAGGATGCAATAGAGACTCTTCTTTTGAAAATGAAAACCCGTATTCAGACATCAATGTTGTAAAGCGCACAACCGTAGTTTCAAACTCGCCAAATGATGGACCAATTAAAGCAATCATTTTCTCCCAATGGACTAAGATGTTGGATTTAGTTGAGAATGCAATGAACGAGTACTGTATACAATACAGAAGGCTTGATGGTACAATGACTCTGACATCAAGAGACAGGGCTGTCAAAGAATTCAACTCTGATCCTGAGGTCACTGTTATGCTTATGTCACTAAAGGCAGGAAACCTTGGTCTAAACATGGTTGCTGCATGTCATGTTATCCTTTTGGATCTTTGGTGGAATCCAACAACTGAGGATCAAGCTGTCGATAGGGCACATAGAATTGGACAAACCCGGCCTGTTACTGTAACTCGACTTACTATTAAGGATACAGTAGAGGACAGGATATTAGCTCTACAGGATGAGAAGAGAAAAATGGTTGCATCTGCTTTTGGTGAAGATAATAGTGGGGGCTCTGCAGCTCGTCTGACAGTTGAGGATCTCAGATACCTATTTATGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

1029

Amino Acids

114.81

Weight (kDa)

7.49

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 287 - 687 1.4e-66 SNF2-related domain
zf-C3HC4_2 PF13923 728 - 764 5.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 728 - 764 8.3e-07 RING-type zinc-finger
zf-C3HC4 PF00097 728 - 764 1.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 728 - 764 4.5e-06 zinc finger of C3HC4-type, RING
Helicase_C PF00271 876 - 971 1.5e-19 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
AasI GACNNNNNNGTC 1 cut(s) 2747
Acc36I ACCTGC 1 cut(s) 2472
AccB1I GGYRCC 1 cut(s) 346
AccB7I CCANNNNNTGG 2 cut(s) 1175, 2615
AccI GTMKAC 1 cut(s) 2695
AciI CCGC 2 cut(s) 782, 1077
AclWI GGATC 7 cut(s) 24, 1715, 2376, 2762, 2862, 2889, 3072
AcoI YGGCCR 1 cut(s) 2230
AcsI RAATTY 6 cut(s) 307, 1045, 1777, 2389, 2497, 2756
AcuI CTGAAG 4 cut(s) 872, 1056, 1122, 1687
AfiI CCNNNNNNNGG 8 cut(s) 522, 734, 1175, 2044, 2365, 2500, 2615, 3082
AjnI CCWGG 1 cut(s) 2160
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AleI CACNNNNGTG 1 cut(s) 107
AloI GAACNNNNNNTCC 2 cut(s) 1282, 1314
Alw26I GTCTC 5 cut(s) 189, 612, 850, 2526, 2733
AlwI GGATC 7 cut(s) 24, 1715, 2376, 2762, 2862, 2889, 3072
AlwNI CAGNNNCTG 1 cut(s) 1175
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 9 cut(s) 573, 943, 1166, 1535, 1617, 1929, 2175, 2230, 2921
ApaI GGGCCC 1 cut(s) 577
ApeKI GCWGC 8 cut(s) 54, 1172, 1284, 1828, 1982, 1985, 2831, 3044
ApoI RAATTY 6 cut(s) 307, 1045, 1777, 2389, 2497, 2756
ArsI GACNNNNNNTTYG 4 cut(s) 2298, 2330, 2525, 2557
AseI ATTAAT 1 cut(s) 1872
AspLEI GCGC 4 cut(s) 1582, 1833, 2037, 2586
AspS9I GGNCC 9 cut(s) 525, 573, 574, 943, 1063, 1535, 1617, 1929, 2618
AsuC2I CCSGG 2 cut(s) 729, 2920
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 3 cut(s) 525, 1063, 2618
AxyI CCTNAGG 1 cut(s) 2772
BaeGI GKGCMC 3 cut(s) 577, 2291, 2902
BaeI ACNNNNGTAYC 2 cut(s) 1170, 1203
BalI TGGCCA 1 cut(s) 2232
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 2 cut(s) 577, 3041
BarI GAAGNNNNNNTAC 2 cut(s) 807, 839
BbvI GCAGC 8 cut(s) 41, 1184, 1296, 1815, 1969, 1972, 2818, 3056
BccI CCATC 8 cut(s) 66, 824, 1641, 1850, 2253, 2369, 2609, 2708
BceAI ACGGC 1 cut(s) 207
BcgI CGANNNNNNTGC 2 cut(s) 1292, 1326
BciT130I CCWGG 1 cut(s) 2162
BciVI GTATCC 1 cut(s) 2947
BclI TGATCA 2 cut(s) 937, 2053
BcnI CCSGG 2 cut(s) 729, 2920
BcoDI GTCTC 5 cut(s) 189, 612, 850, 2526, 2733
BfmI CTRYAG 6 cut(s) 558, 1624, 2479, 2517, 2981, 3042
BfuAI ACCTGC 1 cut(s) 2472
BfuI GTATCC 1 cut(s) 2947
BglII AGATCT 1 cut(s) 202
BisI GCNGC 9 cut(s) 55, 783, 1173, 1285, 1829, 1983, 1986, 2832, 3045
BlsI GCNGC 9 cut(s) 56, 784, 1174, 1286, 1830, 1984, 1987, 2833, 3046
BmcAI AGTACT 1 cut(s) 2690
Bme1390I CCNGG 3 cut(s) 729, 2162, 2920
Bme18I GGWCC 3 cut(s) 525, 1063, 2618
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 9 cut(s) 525, 573, 574, 943, 1063, 1535, 1617, 1929, 2618
BmiI GGNNCC 4 cut(s) 348, 527, 575, 1764
BmrFI CCNGG 3 cut(s) 729, 2162, 2920
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 9 cut(s) 41, 172, 287, 464, 873, 2016, 2127, 2513, 3017
BmuI ACTGGG 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 1110
BpuEI CTTGAG 2 cut(s) 1590, 2225
BpuMI CCSGG 2 cut(s) 729, 2920
Bsa29I ATCGAT 1 cut(s) 2145
BsaAI YACGTR 2 cut(s) 68, 492
BsaBI GATNNNNATC 2 cut(s) 15, 2141
BsaI GGTCTC 2 cut(s) 612, 850
BsaJI CCNNGG 6 cut(s) 946, 1066, 1786, 2160, 2172, 2812
BsaXI ACNNNNNCTCC 2 cut(s) 1901, 1931
Bsc4I CCNNNNNNNGG 8 cut(s) 522, 734, 1175, 2044, 2365, 2500, 2615, 3082
Bse1I ACTGG 5 cut(s) 118, 150, 432, 2275, 2411
Bse21I CCTNAGG 1 cut(s) 2772
Bse3DI GCAATG 3 cut(s) 97, 2200, 2685
Bse8I GATNNNNATC 2 cut(s) 15, 2141
BseBI CCWGG 1 cut(s) 2162
BseCI ATCGAT 1 cut(s) 2145
BseDI CCNNGG 6 cut(s) 946, 1066, 1786, 2160, 2172, 2812
BseJI GATNNNNATC 2 cut(s) 15, 2141
BseLI CCNNNNNNNGG 8 cut(s) 522, 734, 1175, 2044, 2365, 2500, 2615, 3082
BseMI GCAATG 3 cut(s) 97, 2200, 2685
BseMII CTCAG 7 cut(s) 1805, 1889, 1911, 2408, 2763, 2868, 3082
BseNI ACTGG 5 cut(s) 118, 150, 432, 2275, 2411
BseRI GAGGAG 1 cut(s) 1937
BseSI GKGCMC 3 cut(s) 577, 2291, 2902
BseXI GCAGC 8 cut(s) 41, 1184, 1296, 1815, 1969, 1972, 2818, 3056
BsgI GTGCAG 1 cut(s) 1128
BshFI GGCC 9 cut(s) 575, 945, 1168, 1537, 1619, 1931, 2177, 2232, 2923
BshNI GGYRCC 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 2145
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 3 cut(s) 728, 1834, 2920
BslFI GGGAC 1 cut(s) 1346
BslI CCNNNNNNNGG 8 cut(s) 522, 734, 1175, 2044, 2365, 2500, 2615, 3082
BsmAI GTCTC 5 cut(s) 189, 612, 850, 2526, 2733
BsmFI GGGAC 1 cut(s) 1346
BsmI GAATGC 4 cut(s) 666, 2161, 2306, 2680
BsnI GGCC 9 cut(s) 575, 945, 1168, 1537, 1619, 1931, 2177, 2232, 2923
Bso31I GGTCTC 2 cut(s) 612, 850
BsoBI CYCGRG 1 cut(s) 474
Bsp120I GGGCCC 1 cut(s) 573
Bsp1286I GDGCHC 4 cut(s) 577, 2291, 2902, 3041
Bsp19I CCATGG 1 cut(s) 1066
BspACI CCGC 2 cut(s) 782, 1077
BspANI GGCC 9 cut(s) 575, 945, 1168, 1537, 1619, 1931, 2177, 2232, 2923
BspCNI CTCAG 7 cut(s) 1806, 1890, 1912, 2407, 2764, 2869, 3081
BspDI ATCGAT 1 cut(s) 2145
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 4 cut(s) 348, 527, 575, 1764
BspMAI CTGCAG 3 cut(s) 562, 2483, 3046
BspMI ACCTGC 1 cut(s) 2472
BspPI GGATC 7 cut(s) 24, 1715, 2376, 2762, 2862, 2889, 3072
BspT107I GGYRCC 1 cut(s) 346
BspTNI GGTCTC 2 cut(s) 612, 850
BsrDI GCAATG 3 cut(s) 97, 2200, 2685
BsrI ACTGG 5 cut(s) 118, 150, 432, 2275, 2411
BssECI CCNNGG 6 cut(s) 946, 1066, 1786, 2160, 2172, 2812
BssNAI GTATAC 1 cut(s) 2696
BssT1I CCWWGG 4 cut(s) 946, 1066, 1786, 2812
Bst1107I GTATAC 1 cut(s) 2696
Bst2UI CCWGG 1 cut(s) 2162
Bst6I CTCTTC 7 cut(s) 225, 264, 855, 1157, 1445, 2542, 2987
BstAPI GCANNNNNTGC 1 cut(s) 1982
BstBAI YACGTR 2 cut(s) 68, 492
BstC8I GCNNGC 7 cut(s) 546, 908, 1170, 1315, 1621, 1990, 2046
BstDSI CCRYGG 1 cut(s) 1066
BstENI CCTNNNNNAGG 2 cut(s) 2042, 2363
BstHHI GCGC 4 cut(s) 1582, 1833, 2037, 2586
BstMAI GTCTC 5 cut(s) 189, 612, 850, 2526, 2733
BstMWI GCNNNNNNNGC 3 cut(s) 1250, 1982, 2041
BstNI CCWGG 1 cut(s) 2162
BstNSI RCATGY 2 cut(s) 371, 2838
BstSCI CCNGG 3 cut(s) 727, 2160, 2918
BstSFI CTRYAG 6 cut(s) 558, 1624, 2479, 2517, 2981, 3042
BstSLI GKGCMC 3 cut(s) 577, 2291, 2902
BstSNI TACGTA 1 cut(s) 492
BstV1I GCAGC 8 cut(s) 41, 1184, 1296, 1815, 1969, 1972, 2818, 3056
BstX2I RGATCY 4 cut(s) 202, 2368, 2854, 3064
BstYI RGATCY 4 cut(s) 202, 2368, 2854, 3064
BstZ17I GTATAC 1 cut(s) 2696
Bsu15I ATCGAT 1 cut(s) 2145
Bsu36I CCTNAGG 1 cut(s) 2772
BsuI GTATCC 1 cut(s) 2947
BsuRI GGCC 9 cut(s) 575, 945, 1168, 1537, 1619, 1931, 2177, 2232, 2923
BsuTUI ATCGAT 1 cut(s) 2145
BtgI CCRYGG 1 cut(s) 1066
BtsI GCAGTG 1 cut(s) 2476
BtsIMutI CAGTG 6 cut(s) 226, 1220, 2082, 2255, 2476, 2778
BveI ACCTGC 1 cut(s) 2472
Cac8I GCNNGC 7 cut(s) 546, 908, 1170, 1315, 1621, 1990, 2046
CaiI CAGNNNCTG 1 cut(s) 1175
CciI TCATGA 1 cut(s) 25
CfoI GCGC 4 cut(s) 1582, 1833, 2037, 2586
Cfr13I GGNCC 9 cut(s) 525, 573, 574, 943, 1063, 1535, 1617, 1929, 2618
ClaI ATCGAT 1 cut(s) 2145
DraI TTTAAA 1 cut(s) 1117
DrdI GACNNNNNNGTC 1 cut(s) 2747
DseDI GACNNNNNNGTC 1 cut(s) 2747
EaeI YGGCCR 1 cut(s) 2230
Eam1104I CTCTTC 7 cut(s) 225, 264, 855, 1157, 1445, 2542, 2987
EarI CTCTTC 7 cut(s) 225, 264, 855, 1157, 1445, 2542, 2987
Eco105I TACGTA 1 cut(s) 492
Eco130I CCWWGG 4 cut(s) 946, 1066, 1786, 2812
Eco24I GRGCYC 2 cut(s) 577, 3041
Eco31I GGTCTC 2 cut(s) 612, 850
Eco47I GGWCC 3 cut(s) 525, 1063, 2618
Eco57I CTGAAG 4 cut(s) 872, 1056, 1122, 1687
Eco81I CCTNAGG 1 cut(s) 2772
Eco88I CYCGRG 1 cut(s) 474
EcoNI CCTNNNNNAGG 2 cut(s) 2042, 2363
EcoO109I RGGNCCY 5 cut(s) 525, 573, 943, 1617, 1929
EcoRI GAATTC 2 cut(s) 2389, 2756
EcoRII CCWGG 1 cut(s) 2160
EcoT14I CCWWGG 4 cut(s) 946, 1066, 1786, 2812
EcoT38I GRGCYC 2 cut(s) 577, 3041
ErhI CCWWGG 4 cut(s) 946, 1066, 1786, 2812
FaqI GGGAC 1 cut(s) 1346
FauNDI CATATG 3 cut(s) 648, 1339, 1978
FbaI TGATCA 2 cut(s) 937, 2053
FblI GTMKAC 1 cut(s) 2695
Fnu4HI GCNGC 9 cut(s) 55, 783, 1173, 1285, 1829, 1983, 1986, 2832, 3045
FriOI GRGCYC 2 cut(s) 577, 3041
Fsp4HI GCNGC 9 cut(s) 55, 783, 1173, 1285, 1829, 1983, 1986, 2832, 3045
GlaI GCGC 4 cut(s) 1581, 1832, 2036, 2585
GluI GCNGC 9 cut(s) 55, 783, 1173, 1285, 1829, 1983, 1986, 2832, 3045
GsuI CTGGAG 1 cut(s) 1110
HaeIII GGCC 9 cut(s) 575, 945, 1168, 1537, 1619, 1931, 2177, 2232, 2923
HapII CCGG 3 cut(s) 728, 1834, 2920
HhaI GCGC 4 cut(s) 1582, 1833, 2037, 2586
Hin6I GCGC 4 cut(s) 1580, 1831, 2035, 2584
HinP1I GCGC 4 cut(s) 1580, 1831, 2035, 2584
HincII GTYRAC 2 cut(s) 1093, 1444
HindII GTYRAC 2 cut(s) 1093, 1444
HindIII AAGCTT 2 cut(s) 904, 1038
HpaII CCGG 3 cut(s) 728, 1834, 2920
Hpy166II GTNNAC 7 cut(s) 42, 1093, 1444, 1664, 2123, 2433, 2696
Hpy8I GTNNAC 7 cut(s) 42, 1093, 1444, 1664, 2123, 2433, 2696
HpyCH4IV ACGT 2 cut(s) 67, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 1250, 1982, 2041
HpySE526I ACGT 2 cut(s) 67, 491
HspAI GCGC 4 cut(s) 1580, 1831, 2035, 2584
Ksp22I TGATCA 2 cut(s) 937, 2053
LmnI GCTCC 4 cut(s) 160, 1225, 1806, 2503
Lsp1109I GCAGC 8 cut(s) 41, 1184, 1296, 1815, 1969, 1972, 2818, 3056
LweI GCATC 9 cut(s) 41, 172, 287, 464, 873, 2016, 2127, 2513, 3017
MaeII ACGT 2 cut(s) 67, 491
MfeI CAATTG 2 cut(s) 1680, 2295
MflI RGATCY 4 cut(s) 202, 2368, 2854, 3064
MhlI GDGCHC 4 cut(s) 577, 2291, 2902, 3041
MlsI TGGCCA 1 cut(s) 2232
MluNI TGGCCA 1 cut(s) 2232
MlyI GAGTC 2 cut(s) 2528, 2719
MmeI TCCRAC 2 cut(s) 2640, 2894
Mox20I TGGCCA 1 cut(s) 2232
MscI TGGCCA 1 cut(s) 2232
MslI CAYNNNNRTG 5 cut(s) 24, 107, 651, 2250, 2783
Msp20I TGGCCA 1 cut(s) 2232
MspA1I CMGCKG 1 cut(s) 1175
MspI CCGG 3 cut(s) 728, 1834, 2920
MspR9I CCNGG 3 cut(s) 729, 2162, 2920
MunI CAATTG 2 cut(s) 1680, 2295
Mva1269I GAATGC 4 cut(s) 666, 2161, 2306, 2680
MvaI CCWGG 1 cut(s) 2162
MwoI GCNNNNNNNGC 3 cut(s) 1250, 1982, 2041
NciI CCSGG 2 cut(s) 729, 2920
NcoI CCATGG 1 cut(s) 1066
NdeI CATATG 3 cut(s) 648, 1339, 1978
NlaIV GGNNCC 4 cut(s) 348, 527, 575, 1764
NmuCI GTSAC 6 cut(s) 109, 443, 758, 2472, 2776, 2793
NspI RCATGY 2 cut(s) 371, 2838
OliI CACNNNNGTG 1 cut(s) 107
PagI TCATGA 1 cut(s) 25
PctI GAATGC 4 cut(s) 666, 2161, 2306, 2680
PfeI GAWTC 8 cut(s) 924, 1106, 1297, 1594, 1955, 2102, 2339, 2866
PflMI CCANNNNNTGG 2 cut(s) 1175, 2615
PfoI TCCNGGA 1 cut(s) 727
PkrI GCNGC 9 cut(s) 56, 784, 1174, 1286, 1830, 1984, 1987, 2833, 3046
PleI GAGTC 2 cut(s) 2528, 2719
PpsI GAGTC 2 cut(s) 2528, 2719
Ppu21I YACGTR 2 cut(s) 68, 492
PpuMI RGGWCCY 1 cut(s) 525
PshBI ATTAAT 1 cut(s) 1872
PsiI TTATAA 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 2160
PspGI CCWGG 1 cut(s) 2160
PspN4I GGNNCC 4 cut(s) 348, 527, 575, 1764
PspOMI GGGCCC 1 cut(s) 573
PspPI GGNCC 9 cut(s) 525, 573, 574, 943, 1063, 1535, 1617, 1929, 2618
PspPPI RGGWCCY 1 cut(s) 525
PstI CTGCAG 3 cut(s) 562, 2483, 3046
PstNI CAGNNNCTG 1 cut(s) 1175
PsuI RGATCY 4 cut(s) 202, 2368, 2854, 3064
PvuII CAGCTG 1 cut(s) 1175
RseI CAYNNNNRTG 5 cut(s) 24, 107, 651, 2250, 2783
SatI GCNGC 9 cut(s) 55, 783, 1173, 1285, 1829, 1983, 1986, 2832, 3045
Sau96I GGNCC 9 cut(s) 525, 573, 574, 943, 1063, 1535, 1617, 1929, 2618
ScaI AGTACT 1 cut(s) 2690
SchI GAGTC 2 cut(s) 2528, 2719
ScrFI CCNGG 3 cut(s) 729, 2162, 2920
SduI GDGCHC 4 cut(s) 577, 2291, 2902, 3041
SfaNI GCATC 9 cut(s) 41, 172, 287, 464, 873, 2016, 2127, 2513, 3017
SfcI CTRYAG 6 cut(s) 558, 1624, 2479, 2517, 2981, 3042
SinI GGWCC 3 cut(s) 525, 1063, 2618
SmiMI CAYNNNNRTG 5 cut(s) 24, 107, 651, 2250, 2783
SmlI CTYRAG 2 cut(s) 1605, 2204
SmoI CTYRAG 2 cut(s) 1605, 2204
SnaBI TACGTA 1 cut(s) 492
SsiI CCGC 2 cut(s) 782, 1077
SspI AATATT 2 cut(s) 90, 2017
StyD4I CCNGG 3 cut(s) 727, 2160, 2918
StyI CCWWGG 4 cut(s) 946, 1066, 1786, 2812
TaiI ACGT 2 cut(s) 70, 494
TaqI TCGA 6 cut(s) 1001, 1210, 1633, 2145, 2892, 2938
TaqII GACCGA 1 cut(s) 832
TatI WGTACW 5 cut(s) 1264, 2120, 2200, 2430, 2688
TauI GCSGC 1 cut(s) 785
TfiI GAWTC 8 cut(s) 924, 1106, 1297, 1594, 1955, 2102, 2339, 2866
TscAI CASTG 6 cut(s) 226, 1220, 2089, 2255, 2483, 2785
TseFI GTSAC 6 cut(s) 109, 443, 758, 2472, 2776, 2793
TseI GCWGC 8 cut(s) 54, 1172, 1284, 1828, 1982, 1985, 2831, 3044
Tsp45I GTSAC 6 cut(s) 109, 443, 758, 2472, 2776, 2793
TspGWI ACGGA 2 cut(s) 119, 578
TspRI CASTG 6 cut(s) 226, 1220, 2089, 2255, 2483, 2785
Van91I CCANNNNNTGG 2 cut(s) 1175, 2615
VpaK11BI GGWCC 3 cut(s) 525, 1063, 2618
VspI ATTAAT 1 cut(s) 1872
XagI CCTNNNNNAGG 2 cut(s) 2042, 2363
XapI RAATTY 6 cut(s) 307, 1045, 1777, 2389, 2497, 2756
XbaI TCTAGA 2 cut(s) 338, 2129
XceI RCATGY 2 cut(s) 371, 2838
XmiI GTMKAC 1 cut(s) 2695
ZrmI AGTACT 1 cut(s) 2690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.