Rroxscaffold_4G00312050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004881
Physical Location & Seq
Forward (+)
34734017 .. 34742507
8491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312050.1

Sequence Viewer

Length: 645 bp
ATGGACTACCCAAAACCTGTAGAAAACACATTTACACTTCTAATAATACCTACCTTATTCTCCGGAGCCACCCTACTCCCCAAATCTAAACAAGTCCACAATCAAGTTTCACCTGCTACTATCATTGTTGCTGCAATTGATTTATCTATTGGGAAAGATCTACGGCATGCATCACCAGTTCGATGGAGTTCGGTTCTATTAAGCATCATAGACATTTCTGCTCCTAGATTGCATCAACAGGTAGTGGAGCACCTGGATAGAAACAACCACAGTCAGCTTTACAATGTCAAGAAGGAGATTGGCGTCAAGAGAGAAGGTCGGGAAGAAGCCGTGCAGCCTCAACATTTGGAGATGTCCGACGGGAGACGACACCGGCTCCAAGTGAGGAATATTGACTCGCCGCCCCACCGAGTCGACTCGCCGCGGGAATCGGTGGTACCCGAGGAACTAGGAGTCCGGAGAAACGAACGGGTTGAAATGGTGGAAATTCGGGTTCGGCGTCGAGCTAACCGGATGGAACGTCTTCGTGTCGACATCGAAGACCTCCACGTGTCGGGAACTTCGTGTTGCGGCGGAGAGTGGCGACGATTATTCTTTTTCCGTCCGTGCATGGCGGCCGCAGTGAAGCGGTGGAGACCCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

24.74

Weight (kDa)

10.14

Isoelectric Point (pI)

73.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 121
Acc36I ACCTGC 1 cut(s) 121
Acc65I GGTACC 1 cut(s) 436
AccB1I GGYRCC 1 cut(s) 436
AccI GTMKAC 2 cut(s) 414, 531
AccII CGCG 1 cut(s) 424
AccIII TCCGGA 2 cut(s) 62, 456
AciI CCGC 8 cut(s) 401, 422, 424, 570, 573, 614, 618, 628
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 1 cut(s) 486
AcvI CACGTG 1 cut(s) 550
AcyI GRCGYC 2 cut(s) 303, 499
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 1 cut(s) 553
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 1 cut(s) 476
AjnI CCWGG 1 cut(s) 252
AloI GAACNNNNNNTCC 2 cut(s) 438, 470
AluBI AGCT 2 cut(s) 277, 506
AluI AGCT 2 cut(s) 277, 506
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 358, 628
Ama87I CYCGRG 1 cut(s) 440
Aor13HI TCCGGA 2 cut(s) 62, 456
AoxI GGCC 1 cut(s) 615
ApeKI GCWGC 2 cut(s) 131, 334
ApoI RAATTY 1 cut(s) 486
Asp700I GAANNNNTTC 1 cut(s) 522
Asp718I GGTACC 1 cut(s) 436
AsuHPI GGTGA 2 cut(s) 102, 165
AvaI CYCGRG 1 cut(s) 440
BanI GGYRCC 1 cut(s) 436
BbrPI CACGTG 1 cut(s) 550
BbsI GAAGAC 2 cut(s) 515, 546
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 118, 346
BccI CCATC 2 cut(s) 177, 508
BceAI ACGGC 2 cut(s) 179, 314
BciT130I CCWGG 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 358, 628
BfaI CTAG 2 cut(s) 225, 449
BfmI CTRYAG 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 121
BglII AGATCT 1 cut(s) 157
BisI GCNGC 7 cut(s) 132, 335, 401, 422, 571, 615, 618
BlsI GCNGC 7 cut(s) 133, 336, 402, 423, 572, 616, 619
Bme1390I CCNGG 1 cut(s) 254
BmeT110I CYCGRG 1 cut(s) 440
BmiI GGNNCC 3 cut(s) 67, 377, 438
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 3 cut(s) 179, 213, 241
BpiI GAAGAC 2 cut(s) 515, 546
BsaAI YACGTR 1 cut(s) 550
BsaHI GRCGYC 2 cut(s) 303, 499
BsaI GGTCTC 1 cut(s) 628
BsaJI CCNNGG 2 cut(s) 422, 441
BsaWI WCCGGW 3 cut(s) 62, 456, 510
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 553
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 1 cut(s) 176
BseAI TCCGGA 2 cut(s) 62, 456
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 422, 441
BseGI GGATG 1 cut(s) 519
BseLI CCNNNNNNNGG 1 cut(s) 553
BseNI ACTGG 1 cut(s) 176
BseX3I CGGCCG 1 cut(s) 615
BseXI GCAGC 2 cut(s) 118, 346
BsgI GTGCAG 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 424
Bsh1285I CGRYCG 1 cut(s) 618
BshFI GGCC 1 cut(s) 617
BshNI GGYRCC 1 cut(s) 436
BsiEI CGRYCG 1 cut(s) 618
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 4 cut(s) 63, 373, 457, 511
BslI CCNNNNNNNGG 1 cut(s) 553
BsmAI GTCTC 2 cut(s) 358, 628
BsmBI CGTCTC 1 cut(s) 358
BsnI GGCC 1 cut(s) 617
Bso31I GGTCTC 1 cut(s) 628
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 252
Bsp13I TCCGGA 2 cut(s) 62, 456
Bsp143I GATC 1 cut(s) 157
BspACI CCGC 8 cut(s) 401, 422, 424, 570, 573, 614, 618, 628
BspANI GGCC 1 cut(s) 617
BspEI TCCGGA 2 cut(s) 62, 456
BspFNI CGCG 1 cut(s) 424
BspLI GGNNCC 3 cut(s) 67, 377, 438
BspMI ACCTGC 1 cut(s) 121
BspT107I GGYRCC 1 cut(s) 436
BspTNI GGTCTC 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 422, 441
BssMI GATC 1 cut(s) 157
BssNI GRCGYC 2 cut(s) 303, 499
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 272
BstACI GRCGYC 2 cut(s) 303, 499
BstBAI YACGTR 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 168
BstDSI CCRYGG 1 cut(s) 422
BstF5I GGATG 1 cut(s) 519
BstFNI CGCG 1 cut(s) 424
BstKTI GATC 1 cut(s) 160
BstMAI GTCTC 2 cut(s) 358, 628
BstMBI GATC 1 cut(s) 157
BstMCI CGRYCG 1 cut(s) 618
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 252
BstSFI CTRYAG 1 cut(s) 18
BstUI CGCG 1 cut(s) 424
BstV1I GCAGC 2 cut(s) 118, 346
BstV2I GAAGAC 2 cut(s) 515, 546
BstX2I RGATCY 1 cut(s) 157
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 1 cut(s) 157
BstZI CGGCCG 1 cut(s) 615
BsuRI GGCC 1 cut(s) 617
BtgI CCRYGG 1 cut(s) 422
BtsCI GGATG 1 cut(s) 519
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 1 cut(s) 627
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 168
CciNI GCGGCCGC 1 cut(s) 615
Cfr10I RCCGGY 1 cut(s) 372
Cfr42I CCGCGG 1 cut(s) 425
CseI GACGC 2 cut(s) 292, 488
Csp6I GTAC 1 cut(s) 437
CviAII CATG 2 cut(s) 167, 610
CviJI RGCY 7 cut(s) 68, 277, 329, 337, 376, 506, 617
CviKI_1 RGCY 7 cut(s) 68, 277, 329, 337, 376, 506, 617
CviQI GTAC 1 cut(s) 437
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 615
EagI CGGCCG 1 cut(s) 615
EciI GGCGGA 1 cut(s) 588
EclXI CGGCCG 1 cut(s) 615
Eco31I GGTCTC 1 cut(s) 628
Eco52I CGGCCG 1 cut(s) 615
Eco72I CACGTG 1 cut(s) 550
Eco88I CYCGRG 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 252
EcoT22I ATGCAT 1 cut(s) 172
Esp3I CGTCTC 1 cut(s) 358
FaeI CATG 2 cut(s) 170, 613
FaiI YATR 3 cut(s) 168, 209, 611
FatI CATG 2 cut(s) 166, 609
FauI CCCGC 1 cut(s) 417
FblI GTMKAC 2 cut(s) 414, 531
Fnu4HI GCNGC 7 cut(s) 132, 335, 401, 422, 571, 615, 618
FokI GGATG 1 cut(s) 526
Fsp4HI GCNGC 7 cut(s) 132, 335, 401, 422, 571, 615, 618
FspBI CTAG 2 cut(s) 225, 449
GluI GCNGC 7 cut(s) 132, 335, 401, 422, 571, 615, 618
HaeIII GGCC 1 cut(s) 617
HapII CCGG 4 cut(s) 63, 373, 457, 511
HgaI GACGC 2 cut(s) 292, 488
Hin1I GRCGYC 2 cut(s) 303, 499
Hin1II CATG 2 cut(s) 170, 613
HincII GTYRAC 2 cut(s) 415, 532
HindII GTYRAC 2 cut(s) 415, 532
HinfI GANTC 5 cut(s) 395, 411, 416, 428, 453
HpaII CCGG 4 cut(s) 63, 373, 457, 511
HphI GGTGA 2 cut(s) 102, 165
Hpy166II GTNNAC 3 cut(s) 97, 415, 532
Hpy188I TCNGA 1 cut(s) 358
Hpy188III TCNNGA 6 cut(s) 63, 289, 307, 320, 457, 555
Hpy8I GTNNAC 3 cut(s) 97, 415, 532
Hpy99I CGWCG 3 cut(s) 362, 504, 588
HpyAV CCTTC 2 cut(s) 286, 308
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4IV ACGT 2 cut(s) 520, 549
HpyCH4V TGCA 5 cut(s) 134, 170, 232, 334, 609
HpySE526I ACGT 2 cut(s) 520, 549
Hsp92I GRCGYC 2 cut(s) 303, 499
Hsp92II CATG 2 cut(s) 170, 613
Kpn2I TCCGGA 2 cut(s) 62, 456
KpnI GGTACC 1 cut(s) 440
KspI CCGCGG 1 cut(s) 425
Kzo9I GATC 1 cut(s) 157
LmnI GCTCC 4 cut(s) 65, 226, 247, 381
Lsp1109I GCAGC 2 cut(s) 118, 346
LweI GCATC 3 cut(s) 179, 213, 241
MaeI CTAG 2 cut(s) 225, 449
MaeII ACGT 2 cut(s) 520, 549
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 3 cut(s) 335, 515, 551
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 2 cut(s) 135, 486
MlyI GAGTC 4 cut(s) 389, 410, 420, 462
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 4 cut(s) 348, 378, 436, 554
Mph1103I ATGCAT 1 cut(s) 172
MroI TCCGGA 2 cut(s) 62, 456
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 424
MspI CCGG 4 cut(s) 63, 373, 457, 511
MspR9I CCNGG 1 cut(s) 254
MunI CAATTG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 1 cut(s) 424
NdeII GATC 1 cut(s) 157
NlaIII CATG 2 cut(s) 170, 613
NlaIV GGNNCC 3 cut(s) 67, 377, 438
NotI GCGGCCGC 1 cut(s) 615
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 170
PaeI GCATGC 1 cut(s) 170
PaqCI CACCTGC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 2 cut(s) 496, 560
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 428
PkrI GCNGC 7 cut(s) 133, 336, 402, 423, 572, 616, 619
PleI GAGTC 4 cut(s) 389, 410, 419, 461
PmaCI CACGTG 1 cut(s) 550
PmlI CACGTG 1 cut(s) 550
PpsI GAGTC 4 cut(s) 389, 410, 419, 461
Ppu21I YACGTR 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 252
PspCI CACGTG 1 cut(s) 550
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 3 cut(s) 67, 377, 438
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
SacII CCGCGG 1 cut(s) 425
SalI GTCGAC 2 cut(s) 413, 530
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 7 cut(s) 132, 335, 401, 422, 571, 615, 618
Sau3AI GATC 1 cut(s) 157
SchI GAGTC 4 cut(s) 389, 410, 420, 462
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 3 cut(s) 179, 213, 241
SfcI CTRYAG 1 cut(s) 18
Sfr303I CCGCGG 1 cut(s) 425
SgrBI CCGCGG 1 cut(s) 425
SphI GCATGC 1 cut(s) 170
Sse9I AATT 2 cut(s) 135, 486
SsiI CCGC 8 cut(s) 401, 422, 424, 570, 573, 614, 618, 628
SspI AATATT 1 cut(s) 391
SspMI CTAG 2 cut(s) 225, 449
StyD4I CCNGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 272
TaiI ACGT 2 cut(s) 523, 552
TaqI TCGA 5 cut(s) 181, 414, 502, 531, 537
TasI AATT 2 cut(s) 135, 486
TauI GCSGC 5 cut(s) 403, 424, 573, 617, 620
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 1 cut(s) 627
TseI GCWGC 2 cut(s) 131, 334
TspGWI ACGGA 2 cut(s) 590, 594
TspRI CASTG 1 cut(s) 627
XapI RAATTY 1 cut(s) 486
XceI RCATGY 1 cut(s) 170
XmiI GTMKAC 2 cut(s) 414, 531
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 2 cut(s) 225, 449
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.