Rroxscaffold_180G00433520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000180
OG0004881
Physical Location & Seq
Reverse (-)
577186 .. 581504
4319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_180G00433520.1

Sequence Viewer

Length: 798 bp
ATGGTTGAAAGACGCTTTTGCCCCTCCGTTCTGTGCTTGCTTCGACGCCTGGGGTGCTGCTCAGTGCGTGCTGTCTCTTCCTTCGACACCTGGGGTGCTGGTGCTATCGTGTCTTTGCTACTTCACTCCACAAAGAGGGTTGTCGTATTGGGTTTGTCATTAACAAGGAGAAGGAAGGCGATTCTGGGTTATTGGCTCAACCTTGAGGTGGAGGTTTCACCTGCTACTATCATTGTTGCTGCAATTGATTTATCTATTGTAAAAAATTGGTTTGCAAGGTCTGAAAAAAGGAGAAGCTTGAATTTGTCAATCAAGCTGGAGCTATCGGTGGTTCAGTCTTTTACTTGGAGCACCATACCTGGATGTACTTACCGCCAGAGGTACCATGATACTCAATTGATAACTTATCTTTCTGGGTATAATGACTCACAAACCTGGAAAGATCTAAGGCATGCATCACCAGTCCAATGGAGTTCGGTTCTATTAAGCATCATAGACATTTCTGCTTCTAGATTGCATCAACAGGTAATGGAGCACCTGGATGGAAACAACCACAGTCAGCTTTACAATGTCAAGAAGGTGTCTACAACTGAGATACAGAATGGTACTGACGTAGAGTATTCTGCAGAGTCTCAAGCAACAGCATTTGACACAGTTTCAGATAACTACAACTTCAATACACTTCATGCAAGTGGCTTTCTATGGACTATCAGTGACCTCAACAACAATCTGGATCAACATTATCTTCTCATTAGGTTTGATTTCAAGTTAATGATTCTGGTGGAGAAATTCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

265

Amino Acids

30.22

Weight (kDa)

8.79

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 229
Acc36I ACCTGC 1 cut(s) 229
Acc65I GGTACC 1 cut(s) 381
AccB1I GGYRCC 1 cut(s) 381
AccI GTMKAC 1 cut(s) 584
AciI CCGC 1 cut(s) 373
AclWI GGATC 1 cut(s) 741
AcsI RAATTY 2 cut(s) 301, 788
AcyI GRCGYC 1 cut(s) 46
AfaI GTAC 3 cut(s) 367, 383, 607
AfiI CCNNNNNNNGG 2 cut(s) 135, 208
AgsI TTSAA 4 cut(s) 8, 301, 676, 766
AjnI CCWGG 5 cut(s) 48, 89, 358, 434, 537
AluBI AGCT 4 cut(s) 297, 316, 322, 562
AluI AGCT 4 cut(s) 297, 316, 322, 562
Alw21I GWGCWC 2 cut(s) 353, 537
Alw26I GTCTC 2 cut(s) 79, 636
AlwI GGATC 1 cut(s) 741
ApeKI GCWGC 2 cut(s) 57, 239
ApoI RAATTY 2 cut(s) 301, 788
Asp718I GGTACC 1 cut(s) 381
AsuHPI GGTGA 2 cut(s) 210, 450
BaeI ACNNNNGTAYC 2 cut(s) 597, 630
BanI GGYRCC 1 cut(s) 381
Bbv12I GWGCWC 2 cut(s) 353, 537
BbvI GCAGC 2 cut(s) 44, 226
BccI CCATC 1 cut(s) 536
BciT130I CCWGG 5 cut(s) 50, 91, 360, 436, 539
BcoDI GTCTC 2 cut(s) 79, 636
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 229
BglII AGATCT 1 cut(s) 442
BisI GCNGC 2 cut(s) 58, 240
BlsI GCNGC 2 cut(s) 59, 241
Bme1390I CCNGG 5 cut(s) 50, 91, 360, 436, 539
BmiI GGNNCC 1 cut(s) 383
BmrFI CCNGG 5 cut(s) 50, 91, 360, 436, 539
BmsI GCATC 3 cut(s) 464, 498, 526
BpmI CTGGAG 1 cut(s) 338
BpuEI CTTGAG 2 cut(s) 224, 618
BsaHI GRCGYC 1 cut(s) 46
BsaJI CCNNGG 2 cut(s) 49, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 208
Bse1I ACTGG 1 cut(s) 461
BseBI CCWGG 5 cut(s) 50, 91, 360, 436, 539
BseDI CCNNGG 2 cut(s) 49, 90
BseGI GGATG 2 cut(s) 368, 547
BseLI CCNNNNNNNGG 2 cut(s) 135, 208
BseMII CTCAG 2 cut(s) 75, 582
BseNI ACTGG 1 cut(s) 461
BseXI GCAGC 2 cut(s) 44, 226
BshNI GGYRCC 1 cut(s) 381
BsiHKAI GWGCWC 2 cut(s) 353, 537
BslI CCNNNNNNNGG 2 cut(s) 135, 208
BsmAI GTCTC 2 cut(s) 79, 636
Bsp1286I GDGCHC 2 cut(s) 353, 537
Bsp143I GATC 2 cut(s) 442, 733
BspACI CCGC 1 cut(s) 373
BspCNI CTCAG 2 cut(s) 74, 583
BspLI GGNNCC 1 cut(s) 383
BspMAI CTGCAG 1 cut(s) 628
BspMI ACCTGC 1 cut(s) 229
BspPI GGATC 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 381
BsrI ACTGG 1 cut(s) 461
BssECI CCNNGG 2 cut(s) 49, 90
BssMI GATC 2 cut(s) 442, 733
BssNI GRCGYC 1 cut(s) 46
Bst2UI CCWGG 5 cut(s) 50, 91, 360, 436, 539
Bst4CI ACNGT 2 cut(s) 557, 655
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 46
BstC8I GCNNGC 3 cut(s) 38, 69, 453
BstDEI CTNAG 3 cut(s) 61, 446, 591
BstF5I GGATG 2 cut(s) 368, 547
BstKTI GATC 2 cut(s) 445, 736
BstMAI GTCTC 2 cut(s) 79, 636
BstMBI GATC 2 cut(s) 442, 733
BstMWI GCNNNNNNNGC 1 cut(s) 54
BstNI CCWGG 5 cut(s) 50, 91, 360, 436, 539
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 5 cut(s) 48, 89, 358, 434, 537
BstSFI CTRYAG 1 cut(s) 624
BstV1I GCAGC 2 cut(s) 44, 226
BstX2I RGATCY 1 cut(s) 442
BstXI CCANNNNNNTGG 1 cut(s) 468
BstYI RGATCY 1 cut(s) 442
BtsCI GGATG 2 cut(s) 368, 547
BtsIMutI CAGTG 2 cut(s) 69, 718
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 3 cut(s) 38, 69, 453
CseI GACGC 2 cut(s) 21, 54
Csp6I GTAC 3 cut(s) 366, 382, 606
CviAII CATG 3 cut(s) 386, 452, 686
CviJI RGCY 6 cut(s) 196, 297, 316, 322, 562, 696
CviKI_1 RGCY 6 cut(s) 196, 297, 316, 322, 562, 696
CviQI GTAC 3 cut(s) 366, 382, 606
DdeI CTNAG 3 cut(s) 61, 446, 591
DpnI GATC 2 cut(s) 444, 735
DpnII GATC 2 cut(s) 442, 733
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
EcoRII CCWGG 5 cut(s) 48, 89, 358, 434, 537
EcoT22I ATGCAT 1 cut(s) 457
FaeI CATG 3 cut(s) 389, 455, 689
FaiI YATR 7 cut(s) 356, 387, 420, 453, 494, 687, 703
FatI CATG 3 cut(s) 385, 451, 685
FblI GTMKAC 1 cut(s) 584
Fnu4HI GCNGC 2 cut(s) 58, 240
FokI GGATG 2 cut(s) 375, 554
Fsp4HI GCNGC 2 cut(s) 58, 240
FspBI CTAG 1 cut(s) 510
GluI GCNGC 2 cut(s) 58, 240
GsuI CTGGAG 1 cut(s) 338
HgaI GACGC 2 cut(s) 21, 54
Hin1I GRCGYC 1 cut(s) 46
Hin1II CATG 3 cut(s) 389, 455, 689
HindIII AAGCTT 1 cut(s) 295
HinfI GANTC 4 cut(s) 181, 425, 629, 775
HphI GGTGA 2 cut(s) 210, 450
Hpy166II GTNNAC 1 cut(s) 585
Hpy188I TCNGA 2 cut(s) 283, 661
Hpy188III TCNNGA 3 cut(s) 510, 574, 731
Hpy8I GTNNAC 1 cut(s) 585
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 4 cut(s) 91, 165, 169, 571
HpyCH4III ACNGT 2 cut(s) 557, 655
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 6 cut(s) 242, 275, 455, 517, 626, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 54
HpyF3I CTNAG 3 cut(s) 61, 446, 591
HpySE526I ACGT 1 cut(s) 612
Hsp92I GRCGYC 1 cut(s) 46
Hsp92II CATG 3 cut(s) 389, 455, 689
KpnI GGTACC 1 cut(s) 385
Kzo9I GATC 2 cut(s) 442, 733
LmnI GCTCC 3 cut(s) 319, 348, 532
Lsp1109I GCAGC 2 cut(s) 44, 226
LweI GCATC 3 cut(s) 464, 498, 526
MaeI CTAG 1 cut(s) 510
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 1 cut(s) 713
MalI GATC 2 cut(s) 444, 735
MboI GATC 2 cut(s) 442, 733
MboII GAAGA 2 cut(s) 69, 737
MfeI CAATTG 2 cut(s) 243, 395
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 2 cut(s) 353, 537
MluCI AATT 5 cut(s) 243, 265, 301, 395, 788
MlyI GAGTC 2 cut(s) 419, 638
MnlI CCTC 6 cut(s) 34, 129, 199, 205, 372, 728
Mph1103I ATGCAT 1 cut(s) 457
MseI TTAA 3 cut(s) 161, 485, 770
MslI CAYNNNNRTG 2 cut(s) 540, 690
MspR9I CCNGG 5 cut(s) 50, 91, 360, 436, 539
MunI CAATTG 2 cut(s) 243, 395
MvaI CCWGG 5 cut(s) 50, 91, 360, 436, 539
MwoI GCNNNNNNNGC 1 cut(s) 54
NdeII GATC 2 cut(s) 442, 733
NlaIII CATG 3 cut(s) 389, 455, 689
NlaIV GGNNCC 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 713
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 455
PaeI GCATGC 1 cut(s) 455
PaqCI CACCTGC 1 cut(s) 229
PfeI GAWTC 2 cut(s) 181, 775
PkrI GCNGC 2 cut(s) 59, 241
PleI GAGTC 2 cut(s) 419, 637
PpsI GAGTC 2 cut(s) 419, 637
Psp6I CCWGG 5 cut(s) 48, 89, 358, 434, 537
PspGI CCWGG 5 cut(s) 48, 89, 358, 434, 537
PspN4I GGNNCC 1 cut(s) 383
PstI CTGCAG 1 cut(s) 628
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 3 cut(s) 367, 383, 607
RsaNI GTAC 3 cut(s) 366, 382, 606
RseI CAYNNNNRTG 2 cut(s) 540, 690
SaqAI TTAA 3 cut(s) 161, 485, 770
SatI GCNGC 2 cut(s) 58, 240
Sau3AI GATC 2 cut(s) 442, 733
SchI GAGTC 2 cut(s) 419, 638
ScrFI CCNGG 5 cut(s) 50, 91, 360, 436, 539
SduI GDGCHC 2 cut(s) 353, 537
SfaNI GCATC 3 cut(s) 464, 498, 526
SfcI CTRYAG 1 cut(s) 624
SmiMI CAYNNNNRTG 2 cut(s) 540, 690
SmlI CTYRAG 2 cut(s) 203, 633
SmoI CTYRAG 2 cut(s) 203, 633
SphI GCATGC 1 cut(s) 455
Sse9I AATT 5 cut(s) 243, 265, 301, 395, 788
SsiI CCGC 1 cut(s) 373
SspMI CTAG 1 cut(s) 510
StyD4I CCNGG 5 cut(s) 48, 89, 358, 434, 537
TaaI ACNGT 2 cut(s) 557, 655
TaiI ACGT 1 cut(s) 615
TaqI TCGA 2 cut(s) 43, 84
TasI AATT 5 cut(s) 243, 265, 301, 395, 788
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 2 cut(s) 181, 775
Tru1I TTAA 3 cut(s) 161, 485, 770
Tru9I TTAA 3 cut(s) 161, 485, 770
TscAI CASTG 2 cut(s) 69, 718
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 2 cut(s) 57, 239
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 1 cut(s) 674
TspGWI ACGGA 1 cut(s) 16
TspRI CASTG 2 cut(s) 69, 718
XapI RAATTY 2 cut(s) 301, 788
XbaI TCTAGA 1 cut(s) 509
XceI RCATGY 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 584
XspI CTAG 1 cut(s) 510
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.