RLG00000031857

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018812
Physical Location & Seq
Reverse (-)
8799562 .. 8800605
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031857

Sequence Viewer

Length: 1044 bp
ATGTTCATGCCTGTAAGCTGCTCAAACTGCAACACATATCTGCAGTTACCTACAGGTTCTCAGATTGAATGCTGCTCCAGATGCATATTGCGCTTTGCCTACTCAAGGTGTCCTCCTCCTCCTCCTCCCCGATCATACTCCTTCTCCTCTTCCTCCTACAACAACCGCCGTCCTTCCCTGTCATATGCCTTCTCCTTTTTCTCCTACAACAACCGCCCTCCTCCGCCTTCTCGGTTGCCTCGCCAACGCAAGCGAGCCGTGATATGTGGCATAAATTATAAGGGAATGGGGAAGAACATGGAGCTCGAGGGCTGCATTAATGATGCGATTGCAATGCAGAATTTGCTGGTCAGGGAGTTCAAATTCCCTGCGTCTTCCATTCGCATGCTCACTGAAAAAGGAGATCATCAGCCTCCTACCAAAATAAATATAACGATAGCACTGCGTTGGCTTGTGCAAGATTGTCAAGCAGGGGATTCGTTGGTGTTTTATTTTGCCGGTCATGGTGATCAACAGCCCGACAACACAGGAGACGAGGTGGACGAACTAGACGAGACACTTTTGCCCTTAGACTACGAGACCAAGGGAGTGATCGTTGATGATGAGATCAATGAAATGATTGTCCGACCTATTCCGGCAGGTGTCAAGCTCCATGCTATTGTGGATGCTTGCCACAGTGGTACTGTTCTTGATTTGCCATTTCTCTGCAAAATAGACAAAAGCGGCCAGACGTGGTTCTGGAAGGACCATAGACGACCTCAAAAAGCTGGTGTTTGGAAAGGAACGAAAGGTGGAGAGGTCATTTCCTTTAGTGGATGTAATGATCATCAGTGTGCTGTAGAGAAAACAATCCCACGGGGTGCCATGACTTGGGCTTTCATCGATGCCATTGATCGTGGGTGTGGAAATACATATGGCAACTTGTTAATTGCAATGCAATCTACCGTCCTTAAGGATTGTCTTGATACGGATTCCGCCATACAAGAACCACAACTAACTTCAAGTGAAGTATTTGATGTGTTCACAAGGCCTTTCTCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010941 GO:0043067 GO:0043069 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C14 zf-LSD1

Protein Analysis

348

Amino Acids

38.8

Weight (kDa)

6.32

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 83 - 340 1.2e-32 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018812)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g07290
rosa_chinensis RchiOBHm_Chr5g0011711
rosa_laevigata RLG00000031857
rosa_multiflora Rmu_co8248297.1_g000001 Rmu_sc0007176.1_g000004
rosa_roxburghii Rroxscaffold_1G00064810
rosa_rugosa Rorug04G0458600
rosa_samantha Rh5DG085800
rosa_wichuraiana Rw5G007870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 279
AarI CACCTGC 1 cut(s) 629
Acc36I ACCTGC 1 cut(s) 629
AccB1I GGYRCC 1 cut(s) 860
AccB7I CCANNNNNTGG 1 cut(s) 870
AciI CCGC 5 cut(s) 166, 214, 224, 723, 975
AcoI YGGCCR 1 cut(s) 724
AcsI RAATTY 2 cut(s) 340, 362
AdeI CACNNNGTG 1 cut(s) 860
AfaI GTAC 1 cut(s) 682
AfiI CCNNNNNNNGG 2 cut(s) 105, 870
AflII CTTAAG 1 cut(s) 950
AgsI TTSAA 3 cut(s) 68, 361, 1002
AjiI CACGTC 1 cut(s) 732
AluBI AGCT 4 cut(s) 18, 304, 649, 767
AluI AGCT 4 cut(s) 18, 304, 649, 767
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 525, 548, 572
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 2 cut(s) 724, 1028
ApeKI GCWGC 3 cut(s) 18, 72, 312
ApoI RAATTY 2 cut(s) 340, 362
AseI ATTAAT 1 cut(s) 318
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 1 cut(s) 745
AsuHPI GGTGA 1 cut(s) 518
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 745
BanI GGYRCC 1 cut(s) 860
BanII GRGCYC 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 5, 59, 299
BceAI ACGGC 2 cut(s) 153, 242
BcgI CGANNNNNNTGC 6 cut(s) 316, 350, 424, 458, 459, 493
BclI TGATCA 2 cut(s) 508, 823
BcoDI GTCTC 3 cut(s) 525, 548, 572
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 4 cut(s) 41, 51, 837, 1040
BfrI CTTAAG 1 cut(s) 950
BfuAI ACCTGC 1 cut(s) 629
BisI GCNGC 4 cut(s) 19, 73, 313, 724
BlsI GCNGC 4 cut(s) 20, 74, 314, 725
Bme18I GGWCC 1 cut(s) 745
BmeT110I CYCGRG 1 cut(s) 305
BmgBI CACGTC 1 cut(s) 732
BmgT120I GGNCC 1 cut(s) 745
BmiI GGNNCC 1 cut(s) 862
BmsI GCATC 4 cut(s) 71, 313, 655, 874
BpiI GAAGAC 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 88
Bsa29I ATCGAT 1 cut(s) 882
BsaI GGTCTC 1 cut(s) 572
BsaJI CCNNGG 2 cut(s) 582, 854
BsaXI ACNNNNNCTCC 6 cut(s) 128, 158, 522, 552, 579, 609
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 870
Bse118I RCCGGY 1 cut(s) 497
Bse3DI GCAATG 2 cut(s) 339, 939
BseCI ATCGAT 1 cut(s) 882
BseDI CCNNGG 2 cut(s) 582, 854
BseGI GGATG 2 cut(s) 670, 821
BseLI CCNNNNNNNGG 2 cut(s) 105, 870
BseMI GCAATG 2 cut(s) 339, 939
BseMII CTCAG 1 cut(s) 74
BseRI GAGGAG 6 cut(s) 105, 108, 111, 114, 136, 210
BseXI GCAGC 3 cut(s) 5, 59, 299
BshFI GGCC 2 cut(s) 726, 1030
BshNI GGYRCC 1 cut(s) 860
BshVI ATCGAT 1 cut(s) 882
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 2 cut(s) 498, 635
BslI CCNNNNNNNGG 2 cut(s) 105, 870
BsmAI GTCTC 3 cut(s) 525, 548, 572
BsmBI CGTCTC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 2 cut(s) 726, 1030
Bso31I GGTCTC 1 cut(s) 572
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 7 cut(s) 131, 403, 508, 591, 606, 823, 892
BspACI CCGC 5 cut(s) 166, 214, 224, 723, 975
BspANI GGCC 2 cut(s) 726, 1030
BspCNI CTCAG 1 cut(s) 73
BspDI ATCGAT 1 cut(s) 882
BspLI GGNNCC 1 cut(s) 862
BspMAI CTGCAG 1 cut(s) 45
BspMI ACCTGC 1 cut(s) 629
BspT107I GGYRCC 1 cut(s) 860
BspTI CTTAAG 1 cut(s) 950
BspTNI GGTCTC 1 cut(s) 572
BsrDI GCAATG 2 cut(s) 339, 939
BsrFI RCCGGY 1 cut(s) 497
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 2 cut(s) 582, 854
BssMI GATC 7 cut(s) 131, 403, 508, 591, 606, 823, 892
BssT1I CCWWGG 1 cut(s) 582
Bst4CI ACNGT 3 cut(s) 677, 685, 946
Bst6I CTCTTC 1 cut(s) 154
BstAFI CTTAAG 1 cut(s) 950
BstAPI GCANNNNNTGC 1 cut(s) 343
BstC8I GCNNGC 4 cut(s) 251, 255, 386, 670
BstDEI CTNAG 2 cut(s) 60, 568
BstDSI CCRYGG 1 cut(s) 854
BstENI CCTNNNNNAGG 1 cut(s) 103
BstF5I GGATG 2 cut(s) 670, 821
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 7 cut(s) 134, 406, 511, 594, 609, 826, 895
BstMAI GTCTC 3 cut(s) 525, 548, 572
BstMBI GATC 7 cut(s) 131, 403, 508, 591, 606, 823, 892
BstMWI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 343
BstNSI RCATGY 1 cut(s) 388
BstSFI CTRYAG 4 cut(s) 41, 51, 837, 1040
BstV1I GCAGC 3 cut(s) 5, 59, 299
BstV2I GAAGAC 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 882
BsuRI GGCC 2 cut(s) 726, 1030
BsuTUI ATCGAT 1 cut(s) 882
BtgI CCRYGG 1 cut(s) 854
BtrI CACGTC 1 cut(s) 732
BtsCI GGATG 2 cut(s) 670, 821
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 4 cut(s) 390, 440, 682, 836
BveI ACCTGC 1 cut(s) 629
Cac8I GCNNGC 4 cut(s) 251, 255, 386, 670
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 1 cut(s) 745
ClaI ATCGAT 1 cut(s) 882
CseI GACGC 1 cut(s) 360
Csp6I GTAC 1 cut(s) 681
CviAII CATG 6 cut(s) 7, 298, 385, 503, 653, 865
CviQI GTAC 1 cut(s) 681
DdeI CTNAG 2 cut(s) 60, 568
DpnI GATC 7 cut(s) 133, 405, 510, 593, 608, 825, 894
DpnII GATC 7 cut(s) 131, 403, 508, 591, 606, 823, 892
DraIII CACNNNGTG 1 cut(s) 860
EaeI YGGCCR 1 cut(s) 724
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
EciI GGCGGA 2 cut(s) 213, 964
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 582
Eco147I AGGCCT 1 cut(s) 1030
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 572
Eco47I GGWCC 1 cut(s) 745
Eco53kI GAGCTC 1 cut(s) 304
Eco88I CYCGRG 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 304
EcoNI CCTNNNNNAGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 582
EcoT22I ATGCAT 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 582
Esp3I CGTCTC 1 cut(s) 525
FaeI CATG 6 cut(s) 10, 301, 388, 506, 656, 868
FatI CATG 6 cut(s) 6, 297, 384, 502, 652, 864
FauNDI CATATG 2 cut(s) 184, 913
FbaI TGATCA 2 cut(s) 508, 823
Fnu4HI GCNGC 4 cut(s) 19, 73, 313, 724
FokI GGATG 2 cut(s) 677, 828
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 4 cut(s) 19, 73, 313, 724
FspBI CTAG 1 cut(s) 548
GlaI GCGC 1 cut(s) 92
GluI GCNGC 4 cut(s) 19, 73, 313, 724
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 2 cut(s) 726, 1030
HapII CCGG 2 cut(s) 498, 635
HgaI GACGC 1 cut(s) 360
HhaI GCGC 1 cut(s) 93
Hin1II CATG 6 cut(s) 10, 301, 388, 506, 656, 868
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 2 cut(s) 476, 971
HpaII CCGG 2 cut(s) 498, 635
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 2 cut(s) 541, 1023
Hpy188I TCNGA 2 cut(s) 63, 626
Hpy188III TCNNGA 4 cut(s) 78, 689, 739, 962
Hpy8I GTNNAC 2 cut(s) 541, 1023
HpyAV CCTTC 5 cut(s) 151, 183, 199, 237, 736
HpyCH4III ACNGT 3 cut(s) 677, 685, 946
HpyCH4IV ACGT 1 cut(s) 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 343
HpyF3I CTNAG 2 cut(s) 60, 568
HpySE526I ACGT 1 cut(s) 731
Hsp92II CATG 6 cut(s) 10, 301, 388, 506, 656, 868
HspAI GCGC 1 cut(s) 91
Ksp22I TGATCA 2 cut(s) 508, 823
Kzo9I GATC 7 cut(s) 131, 403, 508, 591, 606, 823, 892
LmnI GCTCC 3 cut(s) 80, 301, 654
Lsp1109I GCAGC 3 cut(s) 5, 59, 299
LweI GCATC 4 cut(s) 71, 313, 655, 874
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 731
MaeIII GTNAC 1 cut(s) 45
MalI GATC 7 cut(s) 133, 405, 510, 593, 608, 825, 894
MboI GATC 7 cut(s) 131, 403, 508, 591, 606, 823, 892
MboII GAAGA 3 cut(s) 141, 304, 366
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 4 cut(s) 274, 340, 362, 927
MmeI TCCRAC 1 cut(s) 649
Mph1103I ATGCAT 1 cut(s) 86
MseI TTAA 3 cut(s) 318, 926, 951
MslI CAYNNNNRTG 2 cut(s) 383, 831
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 2 cut(s) 498, 635
Mva1269I GAATGC 1 cut(s) 74
MwoI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 343
NdeI CATATG 2 cut(s) 184, 913
NdeII GATC 7 cut(s) 131, 403, 508, 591, 606, 823, 892
NlaIII CATG 6 cut(s) 10, 301, 388, 506, 656, 868
NlaIV GGNNCC 1 cut(s) 862
NsiI ATGCAT 1 cut(s) 86
NspI RCATGY 1 cut(s) 388
PaeI GCATGC 1 cut(s) 388
PaeR7I CTCGAG 1 cut(s) 305
PaqCI CACCTGC 1 cut(s) 629
PceI AGGCCT 1 cut(s) 1030
PcsI WCGNNNNNNNCGW 2 cut(s) 540, 549
PctI GAATGC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 476, 971
PflMI CCANNNNNTGG 1 cut(s) 870
PkrI GCNGC 4 cut(s) 20, 74, 314, 725
PshBI ATTAAT 1 cut(s) 318
PsiI TTATAA 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 306
PspN4I GGNNCC 1 cut(s) 862
PspPI GGNCC 1 cut(s) 745
PspXI VCTCGAGB 1 cut(s) 305
PstI CTGCAG 1 cut(s) 45
RsaI GTAC 1 cut(s) 682
RsaNI GTAC 1 cut(s) 681
RseI CAYNNNNRTG 2 cut(s) 383, 831
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 3 cut(s) 318, 926, 951
SatI GCNGC 4 cut(s) 19, 73, 313, 724
Sau3AI GATC 7 cut(s) 131, 403, 508, 591, 606, 823, 892
Sau96I GGNCC 1 cut(s) 745
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 4 cut(s) 71, 313, 655, 874
SfcI CTRYAG 4 cut(s) 41, 51, 837, 1040
Sfr274I CTCGAG 1 cut(s) 305
SinI GGWCC 1 cut(s) 745
SlaI CTCGAG 1 cut(s) 305
SmiMI CAYNNNNRTG 2 cut(s) 383, 831
SmlI CTYRAG 3 cut(s) 103, 305, 950
SmoI CTYRAG 3 cut(s) 103, 305, 950
SphI GCATGC 1 cut(s) 388
Sse9I AATT 4 cut(s) 274, 340, 362, 927
SseBI AGGCCT 1 cut(s) 1030
SsiI CCGC 5 cut(s) 166, 214, 224, 723, 975
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 1 cut(s) 306
StuI AGGCCT 1 cut(s) 1030
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 3 cut(s) 677, 685, 946
TaiI ACGT 1 cut(s) 734
TaqI TCGA 2 cut(s) 306, 882
TasI AATT 4 cut(s) 274, 340, 362, 927
TauI GCSGC 1 cut(s) 726
TfiI GAWTC 2 cut(s) 476, 971
Tru1I TTAA 3 cut(s) 318, 926, 951
Tru9I TTAA 3 cut(s) 318, 926, 951
TscAI CASTG 4 cut(s) 397, 447, 682, 836
TseI GCWGC 3 cut(s) 18, 72, 312
TspDTI ATGAA 2 cut(s) 627, 868
TspGWI ACGGA 1 cut(s) 983
TspRI CASTG 4 cut(s) 397, 447, 682, 836
Van91I CCANNNNNTGG 1 cut(s) 870
Vha464I CTTAAG 1 cut(s) 950
VpaK11BI GGWCC 1 cut(s) 745
VspI ATTAAT 1 cut(s) 318
XagI CCTNNNNNAGG 1 cut(s) 103
XapI RAATTY 2 cut(s) 340, 362
XceI RCATGY 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 305
XspI CTAG 1 cut(s) 548
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.