Rh5DG085800

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018812
Physical Location & Seq
Reverse (-)
7527118 .. 7527876
759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG085800.1

Sequence Viewer

Length: 759 bp
ATGGGGAAGAACATGGAGCTCGAGGGCTGCATTAATGATGCGATTGCAATGCAGAATTTGCTGGTCAGGGAGTTCAAATTCCCTGCGTCTTCCATTCGCATGCTTACTGAAAAAGGAGGTCATCAGCCTCCTACCAAAATAAATATAACGATAGCACTGCGTTGGCTTGTGCAAGATTGTCAAGCAGGGGATTCGTTGGTGTTTTATTTTGCCGGTCATGGTGATCAACAGCCCGACAACACAGGAGACGAGGTGGACGAACTAGACGAGACACTTTTGCCCTTAGACTACGAGACTAAGGGAGTGATCGTTGATGATGAGATCAATGAAATGATTGTCCGACCTATTCCGGCCGGTGTCAAGCTCCATGCTATTGTGGATGCTTGCCACAGTGGTACTGTTCTTGATTTGCCATTTCTCTGCAAAATAGACAAAAGCGGCCAGAGGTGGTTCTGGAAGGACCATAGACGACCTCAAAAAGCTGGTGTTTGGAAAGGAACGAAAGGTGGAGAGGTCATTTCCTTTAGTGGATGTAATGATCATCAGTGTGCTGTAGAGAAAACAATCCCACGGGGTGCCATGACTTGGGCTTTCATCGATGCCATTGATCGTGGGTGTGGAAATACATATGGCAACTTGTTAATTGCAATGCAATCTACCGTCCTTAAGGATTGTATTGATACGGATTCCGCCATACAAGAACCACAACTAACTTCAAGTGAAGTATTTGATGTGTTCACAAGGCCTTTCTCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010941 GO:0043067 GO:0043069 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C14 zf-LSD1

Protein Analysis

252

Amino Acids

27.95

Weight (kDa)

4.9

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 5 - 245 8.4e-30 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018812)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g07290
rosa_chinensis RchiOBHm_Chr5g0011711
rosa_laevigata RLG00000031857
rosa_multiflora Rmu_co8248297.1_g000001 Rmu_sc0007176.1_g000004
rosa_roxburghii Rroxscaffold_1G00064810
rosa_rugosa Rorug04G0458600
rosa_samantha Rh5DG085800
rosa_wichuraiana Rw5G007870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 575
AccB7I CCANNNNNTGG 1 cut(s) 585
AciI CCGC 2 cut(s) 438, 690
AcoI YGGCCR 2 cut(s) 351, 439
AcsI RAATTY 2 cut(s) 55, 77
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 1 cut(s) 585
AflII CTTAAG 1 cut(s) 665
AgsI TTSAA 2 cut(s) 76, 717
AluBI AGCT 3 cut(s) 19, 364, 482
AluI AGCT 3 cut(s) 19, 364, 482
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 240, 263, 287
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 3 cut(s) 351, 439, 743
ApeKI GCWGC 1 cut(s) 27
ApoI RAATTY 2 cut(s) 55, 77
AseI ATTAAT 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 460
AsuHPI GGTGA 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 575
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 1 cut(s) 14
BcgI CGANNNNNNTGC 6 cut(s) 31, 65, 139, 173, 174, 208
BclI TGATCA 2 cut(s) 223, 538
BcoDI GTCTC 3 cut(s) 240, 263, 287
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 2 cut(s) 552, 755
BfrI CTTAAG 1 cut(s) 665
BisI GCNGC 2 cut(s) 28, 439
BlsI GCNGC 2 cut(s) 29, 440
Bme18I GGWCC 1 cut(s) 460
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 1 cut(s) 577
BmsI GCATC 3 cut(s) 28, 370, 589
BpiI GAAGAC 1 cut(s) 81
Bsa29I ATCGAT 1 cut(s) 597
BsaJI CCNNGG 1 cut(s) 569
BsaXI ACNNNNNCTCC 4 cut(s) 237, 267, 294, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 585
Bse118I RCCGGY 2 cut(s) 212, 353
Bse3DI GCAATG 2 cut(s) 54, 654
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 1 cut(s) 569
BseGI GGATG 2 cut(s) 385, 536
BseLI CCNNNNNNNGG 1 cut(s) 585
BseMI GCAATG 2 cut(s) 54, 654
BseX3I CGGCCG 1 cut(s) 351
BseXI GCAGC 1 cut(s) 14
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 3 cut(s) 353, 441, 745
BshNI GGYRCC 1 cut(s) 575
BshVI ATCGAT 1 cut(s) 597
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 3 cut(s) 213, 350, 354
BslI CCNNNNNNNGG 1 cut(s) 585
BsmAI GTCTC 3 cut(s) 240, 263, 287
BsmBI CGTCTC 1 cut(s) 240
BsnI GGCC 3 cut(s) 353, 441, 745
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 5 cut(s) 223, 306, 321, 538, 607
BspACI CCGC 2 cut(s) 438, 690
BspANI GGCC 3 cut(s) 353, 441, 745
BspDI ATCGAT 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 577
BspT107I GGYRCC 1 cut(s) 575
BspTI CTTAAG 1 cut(s) 665
BsrDI GCAATG 2 cut(s) 54, 654
BsrFI RCCGGY 2 cut(s) 212, 353
BssAI RCCGGY 2 cut(s) 212, 353
BssECI CCNNGG 1 cut(s) 569
BssMI GATC 5 cut(s) 223, 306, 321, 538, 607
Bst4CI ACNGT 3 cut(s) 392, 400, 661
BstAFI CTTAAG 1 cut(s) 665
BstAPI GCANNNNNTGC 1 cut(s) 58
BstC8I GCNNGC 2 cut(s) 101, 385
BstDEI CTNAG 2 cut(s) 283, 297
BstDSI CCRYGG 1 cut(s) 569
BstF5I GGATG 2 cut(s) 385, 536
BstKTI GATC 5 cut(s) 226, 309, 324, 541, 610
BstMAI GTCTC 3 cut(s) 240, 263, 287
BstMBI GATC 5 cut(s) 223, 306, 321, 538, 607
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 103
BstSFI CTRYAG 2 cut(s) 552, 755
BstV1I GCAGC 1 cut(s) 14
BstV2I GAAGAC 1 cut(s) 81
BstZI CGGCCG 1 cut(s) 351
Bsu15I ATCGAT 1 cut(s) 597
BsuRI GGCC 3 cut(s) 353, 441, 745
BsuTUI ATCGAT 1 cut(s) 597
BtgI CCRYGG 1 cut(s) 569
BtsCI GGATG 2 cut(s) 385, 536
BtsI GCAGTG 1 cut(s) 155
BtsIMutI CAGTG 3 cut(s) 155, 397, 551
Cac8I GCNNGC 2 cut(s) 101, 385
Cfr10I RCCGGY 2 cut(s) 212, 353
Cfr13I GGNCC 1 cut(s) 460
ClaI ATCGAT 1 cut(s) 597
CseI GACGC 1 cut(s) 75
Csp6I GTAC 1 cut(s) 396
CviAII CATG 5 cut(s) 13, 100, 218, 368, 580
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 2 cut(s) 283, 297
DpnI GATC 5 cut(s) 225, 308, 323, 540, 609
DpnII GATC 5 cut(s) 223, 306, 321, 538, 607
DraIII CACNNNGTG 1 cut(s) 575
EaeI YGGCCR 2 cut(s) 351, 439
EagI CGGCCG 1 cut(s) 351
EciI GGCGGA 1 cut(s) 679
Ecl136II GAGCTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 351
Eco147I AGGCCT 1 cut(s) 745
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 460
Eco52I CGGCCG 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 19
EcoT38I GRGCYC 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 240
FaeI CATG 5 cut(s) 16, 103, 221, 371, 583
FatI CATG 5 cut(s) 12, 99, 217, 367, 579
FauNDI CATATG 1 cut(s) 628
FbaI TGATCA 2 cut(s) 223, 538
Fnu4HI GCNGC 2 cut(s) 28, 439
FokI GGATG 2 cut(s) 392, 543
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 2 cut(s) 28, 439
FspBI CTAG 1 cut(s) 263
GluI GCNGC 2 cut(s) 28, 439
HaeIII GGCC 3 cut(s) 353, 441, 745
HapII CCGG 3 cut(s) 213, 350, 354
HgaI GACGC 1 cut(s) 75
Hin1II CATG 5 cut(s) 16, 103, 221, 371, 583
HinfI GANTC 2 cut(s) 191, 686
HpaII CCGG 3 cut(s) 213, 350, 354
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 2 cut(s) 256, 738
Hpy188I TCNGA 1 cut(s) 341
Hpy188III TCNNGA 2 cut(s) 404, 454
Hpy8I GTNNAC 2 cut(s) 256, 738
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 3 cut(s) 392, 400, 661
HpyCH4V TGCA 7 cut(s) 30, 47, 52, 172, 423, 647, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpyF3I CTNAG 2 cut(s) 283, 297
Hsp92II CATG 5 cut(s) 16, 103, 221, 371, 583
Ksp22I TGATCA 2 cut(s) 223, 538
Kzo9I GATC 5 cut(s) 223, 306, 321, 538, 607
LmnI GCTCC 2 cut(s) 16, 369
Lsp1109I GCAGC 1 cut(s) 14
LweI GCATC 3 cut(s) 28, 370, 589
MaeI CTAG 1 cut(s) 263
MalI GATC 5 cut(s) 225, 308, 323, 540, 609
MboI GATC 5 cut(s) 223, 306, 321, 538, 607
MboII GAAGA 2 cut(s) 19, 81
MhlI GDGCHC 1 cut(s) 21
MluCI AATT 3 cut(s) 55, 77, 642
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 7 cut(s) 16, 110, 138, 244, 438, 483, 505
MseI TTAA 3 cut(s) 33, 641, 666
MslI CAYNNNNRTG 2 cut(s) 98, 546
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 3 cut(s) 213, 350, 354
MwoI GCNNNNNNNGC 1 cut(s) 58
NdeI CATATG 1 cut(s) 628
NdeII GATC 5 cut(s) 223, 306, 321, 538, 607
NlaIII CATG 5 cut(s) 16, 103, 221, 371, 583
NlaIV GGNNCC 1 cut(s) 577
NspI RCATGY 1 cut(s) 103
PaeI GCATGC 1 cut(s) 103
PaeR7I CTCGAG 1 cut(s) 20
PceI AGGCCT 1 cut(s) 745
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 264
PfeI GAWTC 2 cut(s) 191, 686
PflMI CCANNNNNTGG 1 cut(s) 585
PkrI GCNGC 2 cut(s) 29, 440
PshBI ATTAAT 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 577
PspPI GGNCC 1 cut(s) 460
PspXI VCTCGAGB 1 cut(s) 20
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
RseI CAYNNNNRTG 2 cut(s) 98, 546
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 3 cut(s) 33, 641, 666
SatI GCNGC 2 cut(s) 28, 439
Sau3AI GATC 5 cut(s) 223, 306, 321, 538, 607
Sau96I GGNCC 1 cut(s) 460
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 3 cut(s) 28, 370, 589
SfcI CTRYAG 2 cut(s) 552, 755
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 1 cut(s) 460
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 2 cut(s) 98, 546
SmlI CTYRAG 2 cut(s) 20, 665
SmoI CTYRAG 2 cut(s) 20, 665
SphI GCATGC 1 cut(s) 103
Sse9I AATT 3 cut(s) 55, 77, 642
SseBI AGGCCT 1 cut(s) 745
SsiI CCGC 2 cut(s) 438, 690
SspMI CTAG 1 cut(s) 263
SstI GAGCTC 1 cut(s) 21
StuI AGGCCT 1 cut(s) 745
TaaI ACNGT 3 cut(s) 392, 400, 661
TaqI TCGA 2 cut(s) 21, 597
TasI AATT 3 cut(s) 55, 77, 642
TauI GCSGC 1 cut(s) 441
TfiI GAWTC 2 cut(s) 191, 686
Tru1I TTAA 3 cut(s) 33, 641, 666
Tru9I TTAA 3 cut(s) 33, 641, 666
TscAI CASTG 3 cut(s) 162, 397, 551
TseI GCWGC 1 cut(s) 27
TspDTI ATGAA 2 cut(s) 342, 583
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 3 cut(s) 162, 397, 551
Van91I CCANNNNNTGG 1 cut(s) 585
Vha464I CTTAAG 1 cut(s) 665
VpaK11BI GGWCC 1 cut(s) 460
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 2 cut(s) 55, 77
XceI RCATGY 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.