Rw5G007870

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018812
Physical Location & Seq
Reverse (-)
8366444 .. 8367490
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G007870.1

Sequence Viewer

Length: 1047 bp
ATGTTCATGCCTGTAAACTGCTCAAACTGCAACACATATCTGCAGTTACCTACAGGTTCTCAGATTGTATGCTGCTCCAGATGCATATTGCGCTTTGCCTACTCAAGGTGTCCTCCTCCTCCTCCTCCTCCCCGGTCATACTCCTTCTCCTCTTCCTCCTACAACAACCGCCGTCCTTCCCTGTCATATGCCTTCTCCTTTTTCTCCTACAACAACGGCCCTCCTCCGCCTTCTCGGTTGCCTCGCCAACGCAAGCGAGCCGTGATATGTGGCATAAATTATAAGGGAATGGGGAAGAACATGGAGCTCGAGGGCTGCATTAATGATGCGATTGCAATGCAGAATTTGCTGGTCAGTGAGTTCAAATTCCCTGCGTCTTCCATTCGCATGCTTACTGAAAAAGGAGGTCATCAGCCTCCTACCAAAATAAATATAACGATAGCACTGCGTTGGCTTGTGCAAGATTGTCAAGCAGGGGATTCGTTGGTGTTTTATTTTGCCGGTCATGGTGATCAACAGCCCGACAACACAGGAGACGAGGTGGACGAACTAGAAGAGACACTTTTGCCCTTAGACTACGAGACTAAGGGAGTGATCGTTGATGATGAGATCAATGAAATGATTGTCCGACCTATTCCGGCCGGTGTCAAGCTCCATGCTATTGTGGATGCTTGCCACAGTGGTACTGTTCTTGATTTGCCATTTCTCTGCAAAATAGACAAAAGCGGCCAGAGGTGGTTCTGGAAGGACCATAGACGACCTCAAAAAGCTGGTGTTTGGAAAGGAACGAAAGGTGGAGAGGTCATTTCCTTTAGTGGATGTAATGATCATCAGTGTGCTGTAGAGAAAACAATCCCACGGGGTGCCATGACTTGGGCTTTCATCGATGCCATTGATCGTGGGTGTGGAAATACATATGGCAACTTGTTAATTGCAATGCAATCTACCGTCCTTAAGGATTGTATTGATACGGATTCCGCCATACAAGAACCACAACTAACTTCAAGTGAAGTATTTGATGTGTTCACAAGGCCTTTCTCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010941 GO:0043067 GO:0043069 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C14 zf-LSD1

Protein Analysis

348

Amino Acids

38.74

Weight (kDa)

6.59

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 84 - 341 3.7e-32 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018812)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g07290
rosa_chinensis RchiOBHm_Chr5g0011711
rosa_laevigata RLG00000031857
rosa_multiflora Rmu_co8248297.1_g000001 Rmu_sc0007176.1_g000004
rosa_roxburghii Rroxscaffold_1G00064810
rosa_rugosa Rorug04G0458600
rosa_samantha Rh5DG085800
rosa_wichuraiana Rw5G007870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 863
AccB7I CCANNNNNTGG 1 cut(s) 873
AciI CCGC 4 cut(s) 169, 227, 726, 978
AcoI YGGCCR 2 cut(s) 639, 727
AcsI RAATTY 2 cut(s) 343, 365
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 1 cut(s) 685
AfiI CCNNNNNNNGG 2 cut(s) 105, 873
AflII CTTAAG 1 cut(s) 953
AgsI TTSAA 2 cut(s) 364, 1005
AluBI AGCT 3 cut(s) 307, 652, 770
AluI AGCT 3 cut(s) 307, 652, 770
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 3 cut(s) 528, 551, 575
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 4 cut(s) 217, 639, 727, 1031
ApeKI GCWGC 2 cut(s) 72, 315
ApoI RAATTY 2 cut(s) 343, 365
AseI ATTAAT 1 cut(s) 321
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 218, 748
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 1 cut(s) 309
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 59, 302
BceAI ACGGC 3 cut(s) 156, 232, 245
BcgI CGANNNNNNTGC 6 cut(s) 319, 353, 427, 461, 462, 496
BclI TGATCA 2 cut(s) 511, 826
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 3 cut(s) 528, 551, 575
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 4 cut(s) 41, 51, 840, 1043
BfrI CTTAAG 1 cut(s) 953
BisI GCNGC 3 cut(s) 73, 316, 727
BlsI GCNGC 3 cut(s) 74, 317, 728
Bme1390I CCNGG 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 748
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 218, 748
BmiI GGNNCC 1 cut(s) 865
BmrFI CCNGG 1 cut(s) 133
BmsI GCATC 4 cut(s) 71, 316, 658, 877
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 885
BsaJI CCNNGG 2 cut(s) 131, 857
BsaXI ACNNNNNCTCC 6 cut(s) 131, 161, 525, 555, 582, 612
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 873
Bse118I RCCGGY 2 cut(s) 500, 641
Bse3DI GCAATG 2 cut(s) 342, 942
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 2 cut(s) 131, 857
BseGI GGATG 2 cut(s) 673, 824
BseLI CCNNNNNNNGG 2 cut(s) 105, 873
BseMI GCAATG 2 cut(s) 342, 942
BseMII CTCAG 1 cut(s) 74
BseRI GAGGAG 7 cut(s) 105, 108, 111, 114, 117, 139, 213
BseX3I CGGCCG 1 cut(s) 639
BseXI GCAGC 2 cut(s) 59, 302
Bsh1285I CGRYCG 1 cut(s) 642
BshFI GGCC 4 cut(s) 219, 641, 729, 1033
BshNI GGYRCC 1 cut(s) 863
BshVI ATCGAT 1 cut(s) 885
BsiEI CGRYCG 1 cut(s) 642
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 4 cut(s) 133, 501, 638, 642
BslI CCNNNNNNNGG 2 cut(s) 105, 873
BsmAI GTCTC 3 cut(s) 528, 551, 575
BsmBI CGTCTC 1 cut(s) 528
BsnI GGCC 4 cut(s) 219, 641, 729, 1033
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 5 cut(s) 511, 594, 609, 826, 895
BspACI CCGC 4 cut(s) 169, 227, 726, 978
BspANI GGCC 4 cut(s) 219, 641, 729, 1033
BspCNI CTCAG 1 cut(s) 73
BspDI ATCGAT 1 cut(s) 885
BspLI GGNNCC 1 cut(s) 865
BspMAI CTGCAG 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 863
BspTI CTTAAG 1 cut(s) 953
BsrDI GCAATG 2 cut(s) 342, 942
BsrFI RCCGGY 2 cut(s) 500, 641
BssAI RCCGGY 2 cut(s) 500, 641
BssECI CCNNGG 2 cut(s) 131, 857
BssMI GATC 5 cut(s) 511, 594, 609, 826, 895
Bst4CI ACNGT 3 cut(s) 680, 688, 949
Bst6I CTCTTC 2 cut(s) 157, 549
BstAFI CTTAAG 1 cut(s) 953
BstAPI GCANNNNNTGC 1 cut(s) 346
BstC8I GCNNGC 4 cut(s) 254, 258, 389, 673
BstDEI CTNAG 3 cut(s) 60, 571, 585
BstDSI CCRYGG 1 cut(s) 857
BstENI CCTNNNNNAGG 1 cut(s) 103
BstF5I GGATG 2 cut(s) 673, 824
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 5 cut(s) 514, 597, 612, 829, 898
BstMAI GTCTC 3 cut(s) 528, 551, 575
BstMBI GATC 5 cut(s) 511, 594, 609, 826, 895
BstMCI CGRYCG 1 cut(s) 642
BstMWI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 346
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 1 cut(s) 131
BstSFI CTRYAG 4 cut(s) 41, 51, 840, 1043
BstV1I GCAGC 2 cut(s) 59, 302
BstV2I GAAGAC 1 cut(s) 369
BstZI CGGCCG 1 cut(s) 639
Bsu15I ATCGAT 1 cut(s) 885
BsuRI GGCC 4 cut(s) 219, 641, 729, 1033
BsuTUI ATCGAT 1 cut(s) 885
BtgI CCRYGG 1 cut(s) 857
BtsCI GGATG 2 cut(s) 673, 824
BtsI GCAGTG 1 cut(s) 443
BtsIMutI CAGTG 4 cut(s) 361, 443, 685, 839
Cac8I GCNNGC 4 cut(s) 254, 258, 389, 673
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 2 cut(s) 500, 641
Cfr13I GGNCC 2 cut(s) 218, 748
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 1 cut(s) 363
Csp6I GTAC 1 cut(s) 684
CviAII CATG 6 cut(s) 7, 301, 388, 506, 656, 868
CviQI GTAC 1 cut(s) 684
DdeI CTNAG 3 cut(s) 60, 571, 585
DpnI GATC 5 cut(s) 513, 596, 611, 828, 897
DpnII GATC 5 cut(s) 511, 594, 609, 826, 895
DraIII CACNNNGTG 1 cut(s) 863
EaeI YGGCCR 2 cut(s) 639, 727
EagI CGGCCG 1 cut(s) 639
Eam1104I CTCTTC 2 cut(s) 157, 549
EarI CTCTTC 2 cut(s) 157, 549
EciI GGCGGA 2 cut(s) 216, 967
Ecl136II GAGCTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 639
Eco147I AGGCCT 1 cut(s) 1033
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 748
Eco52I CGGCCG 1 cut(s) 639
Eco53kI GAGCTC 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 307
EcoNI CCTNNNNNAGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 528
FaeI CATG 6 cut(s) 10, 304, 391, 509, 659, 871
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FatI CATG 6 cut(s) 6, 300, 387, 505, 655, 867
FauNDI CATATG 2 cut(s) 187, 916
FbaI TGATCA 2 cut(s) 511, 826
Fnu4HI GCNGC 3 cut(s) 73, 316, 727
FokI GGATG 2 cut(s) 680, 831
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 3 cut(s) 73, 316, 727
FspBI CTAG 1 cut(s) 551
GlaI GCGC 1 cut(s) 92
GluI GCNGC 3 cut(s) 73, 316, 727
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 4 cut(s) 219, 641, 729, 1033
HapII CCGG 4 cut(s) 133, 501, 638, 642
HgaI GACGC 1 cut(s) 363
HhaI GCGC 1 cut(s) 93
Hin1II CATG 6 cut(s) 10, 304, 391, 509, 659, 871
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 2 cut(s) 479, 974
HpaII CCGG 4 cut(s) 133, 501, 638, 642
HphI GGTGA 1 cut(s) 521
Hpy166II GTNNAC 3 cut(s) 16, 544, 1026
Hpy188I TCNGA 2 cut(s) 63, 629
Hpy188III TCNNGA 3 cut(s) 78, 692, 742
Hpy8I GTNNAC 3 cut(s) 16, 544, 1026
HpyAV CCTTC 5 cut(s) 154, 186, 202, 240, 739
HpyCH4III ACNGT 3 cut(s) 680, 688, 949
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 346
HpyF3I CTNAG 3 cut(s) 60, 571, 585
Hsp92II CATG 6 cut(s) 10, 304, 391, 509, 659, 871
HspAI GCGC 1 cut(s) 91
Ksp22I TGATCA 2 cut(s) 511, 826
Kzo9I GATC 5 cut(s) 511, 594, 609, 826, 895
LmnI GCTCC 3 cut(s) 80, 304, 657
Lsp1109I GCAGC 2 cut(s) 59, 302
LweI GCATC 4 cut(s) 71, 316, 658, 877
MaeI CTAG 1 cut(s) 551
MaeIII GTNAC 1 cut(s) 45
MalI GATC 5 cut(s) 513, 596, 611, 828, 897
MboI GATC 5 cut(s) 511, 594, 609, 826, 895
MboII GAAGA 4 cut(s) 144, 307, 369, 566
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 4 cut(s) 277, 343, 365, 930
MmeI TCCRAC 1 cut(s) 652
Mph1103I ATGCAT 1 cut(s) 86
MseI TTAA 3 cut(s) 321, 929, 954
MslI CAYNNNNRTG 2 cut(s) 386, 834
MspCI CTTAAG 1 cut(s) 953
MspI CCGG 4 cut(s) 133, 501, 638, 642
MspR9I CCNGG 1 cut(s) 133
MwoI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 346
NciI CCSGG 1 cut(s) 133
NdeI CATATG 2 cut(s) 187, 916
NdeII GATC 5 cut(s) 511, 594, 609, 826, 895
NlaIII CATG 6 cut(s) 10, 304, 391, 509, 659, 871
NlaIV GGNNCC 1 cut(s) 865
NsiI ATGCAT 1 cut(s) 86
NspI RCATGY 1 cut(s) 391
PaeI GCATGC 1 cut(s) 391
PaeR7I CTCGAG 1 cut(s) 308
PceI AGGCCT 1 cut(s) 1033
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PfeI GAWTC 2 cut(s) 479, 974
PflMI CCANNNNNTGG 1 cut(s) 873
PkrI GCNGC 3 cut(s) 74, 317, 728
PshBI ATTAAT 1 cut(s) 321
PsiI TTATAA 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 309
PspN4I GGNNCC 1 cut(s) 865
PspPI GGNCC 2 cut(s) 218, 748
PspXI VCTCGAGB 1 cut(s) 308
PstI CTGCAG 1 cut(s) 45
RsaI GTAC 1 cut(s) 685
RsaNI GTAC 1 cut(s) 684
RseI CAYNNNNRTG 2 cut(s) 386, 834
SacI GAGCTC 1 cut(s) 309
SaqAI TTAA 3 cut(s) 321, 929, 954
SatI GCNGC 3 cut(s) 73, 316, 727
Sau3AI GATC 5 cut(s) 511, 594, 609, 826, 895
Sau96I GGNCC 2 cut(s) 218, 748
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 4 cut(s) 71, 316, 658, 877
SfcI CTRYAG 4 cut(s) 41, 51, 840, 1043
Sfr274I CTCGAG 1 cut(s) 308
SinI GGWCC 1 cut(s) 748
SlaI CTCGAG 1 cut(s) 308
SmiMI CAYNNNNRTG 2 cut(s) 386, 834
SmlI CTYRAG 3 cut(s) 103, 308, 953
SmoI CTYRAG 3 cut(s) 103, 308, 953
SphI GCATGC 1 cut(s) 391
Sse9I AATT 4 cut(s) 277, 343, 365, 930
SseBI AGGCCT 1 cut(s) 1033
SsiI CCGC 4 cut(s) 169, 227, 726, 978
SspMI CTAG 1 cut(s) 551
SstI GAGCTC 1 cut(s) 309
StuI AGGCCT 1 cut(s) 1033
StyD4I CCNGG 1 cut(s) 131
TaaI ACNGT 3 cut(s) 680, 688, 949
TaqI TCGA 2 cut(s) 309, 885
TasI AATT 4 cut(s) 277, 343, 365, 930
TauI GCSGC 1 cut(s) 729
TfiI GAWTC 2 cut(s) 479, 974
Tru1I TTAA 3 cut(s) 321, 929, 954
Tru9I TTAA 3 cut(s) 321, 929, 954
TscAI CASTG 4 cut(s) 361, 450, 685, 839
TseI GCWGC 2 cut(s) 72, 315
TspDTI ATGAA 2 cut(s) 630, 871
TspGWI ACGGA 1 cut(s) 986
TspRI CASTG 4 cut(s) 361, 450, 685, 839
Van91I CCANNNNNTGG 1 cut(s) 873
Vha464I CTTAAG 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 748
VspI ATTAAT 1 cut(s) 321
XagI CCTNNNNNAGG 1 cut(s) 103
XapI RAATTY 2 cut(s) 343, 365
XceI RCATGY 1 cut(s) 391
XhoI CTCGAG 1 cut(s) 308
XspI CTAG 1 cut(s) 551
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.