FvH4_3g14040

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015065
Physical Location & Seq
Forward (+)
8436917 .. 8439867
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14040.t1

Sequence Viewer

Length: 1185 bp
ATGATGAGTTCGAAGGCGAGGAAGAAGCAGATGTGGTCTCCGCAGCCATTGACGCCACTCATGGAAGGTCCGGACCCAGAAATCCAAGAAGAAGGTGGTAAGAAGGAGAGCTCTTGGGAAGCCATACGTGATTGGTTCAGAGTACAAAAGGGTCTTCCTCAAGGAAGTAGCTTATCAACTTCAGCATATGGTAGTAATGGTAGTACTGTTCCGGCAAAGAGACAAGATTTAAGGCTCTTGCTTGGAGTTCTGGGATGCCCTCTAGCACCTATTCCTCAAGCCAATGATCCAGCTGATCATCCCATGCCCGCTCACATCAAAGACATCCCATTCGAAACTTCGACTGCGCATTACATTATACAACAGTATCTGGCAGCAAGTGGTTGCTTGAAGCAGCAGAAGTGCACCAAGAACATGTACGCGAGTGGGATGGTGAAGATGGTTTGCTGTGAGACTGAAGTTTCTGCAGGTAGAAACGTCAAGACTTTGGGAACAAGAAGTGGGGAAAGTGGGTGCTTCGTTCTCTGGCAAATGCTGCCCGGAATGTGGTCTCTGGAACTGGTGGTCGGCGGCAACAAGGTGGTCGCCGGAAGTGATGGGAAAACTGTATGGAGGCACACCCCTTGGCTCGGTACTCATGCCGCCAAAGGCCCTCAACGCCCATTACGCCGTATCATCCAGGGCCTAGACCCAAAGAGCACAGCAAGCTTGTTTGCCAAGGCACAATGCTTGGGGGAGAAGAGGATTGGCAACGATGATTGCTTCGTACTAAAAGTCTCTGCAGATCGCGAGGCGGTTATAGAACGAAGCGAAGGTCCGGCAGAGGTAATTAGGCATGCATTGTACGGCTATTTCTGTCAAAAGAGTGGACTCCTCATCTACTTAGAGGACTCTCACCTTACTAGGGTTGAAACACCAGAAAACGAAACTGTGTATTGGGAGACCACTATAGGAAGCAGCATTGCAGATTACAGAGATGTTGATGGTGTGCTCATCGCTCATCAAGGCCGTTCCATCGCCACTGTATTCCGGTGTGGCGAGCTATCAATGGAGTACACGAGGACTAGAATGGAAGAGGTTTGGAACATTGATGATGTTGTGTTCAATGTGCCGGGGCTTTCCATGGACTATTTCATTGCTCCTGCTGATATATATGATAGTAGTAGTGCTGTACAATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

395

Amino Acids

43.45

Weight (kDa)

6.27

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 139 - 381 3.3e-111 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015065)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79420
fragaria_vesca FvH4_3g14040
malus_domestica MD05G1238500.v1.1 MD10G1216800.v1.1
prunus_persica Prupe.4G127900_v2.0.a1
pyrus_communis pycom05g21460
rosa_chinensis RchiOBHm_Chr5g0023361
rosa_laevigata RLG00000032716
rosa_multiflora Rmu_sc0007223.1_g000005
rosa_roxburghii Rroxscaffold_1G00055980
rosa_rugosa Rorug05G0078600
rosa_samantha Rh5AG165500 Rh5BG164700 Rh5CG179600 Rh5DG166200
rosa_wichuraiana Rw5G014890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 348
Acc36I ACCTGC 1 cut(s) 458
AccBSI CCGCTC 1 cut(s) 311
AccII CGCG 2 cut(s) 422, 789
AccIII TCCGGA 1 cut(s) 70
AciI CCGC 5 cut(s) 41, 309, 570, 642, 794
AclWI GGATC 1 cut(s) 281
AcuI CTGAAG 2 cut(s) 165, 477
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 8 cut(s) 144, 205, 419, 634, 768, 845, 1055, 1173
AfiI CCNNNNNNNGG 3 cut(s) 546, 629, 904
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 3 cut(s) 391, 911, 1105
AjnI CCWGG 1 cut(s) 678
AluBI AGCT 5 cut(s) 111, 171, 293, 708, 1042
AluI AGCT 5 cut(s) 111, 171, 293, 708, 1042
Alw21I GWGCWC 4 cut(s) 113, 407, 701, 993
Alw26I GTCTC 6 cut(s) 42, 214, 446, 555, 781, 935
Alw44I GTGCAC 1 cut(s) 403
AlwI GGATC 1 cut(s) 281
AlwNI CAGNNNCTG 1 cut(s) 370
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 3 cut(s) 649, 682, 1006
ApaLI GTGCAC 1 cut(s) 403
ApeKI GCWGC 5 cut(s) 43, 374, 394, 535, 957
ArsI GACNNNNNNTTYG 4 cut(s) 314, 346, 799, 831
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 5 cut(s) 68, 73, 650, 682, 815
AsuC2I CCSGG 2 cut(s) 540, 1113
AsuHPI GGTGA 3 cut(s) 445, 887, 1170
AsuII TTCGAA 2 cut(s) 11, 333
AvaII GGWCC 3 cut(s) 68, 73, 815
BaeGI GKGCMC 1 cut(s) 407
BaeI ACNNNNGTAYC 2 cut(s) 350, 383
BanII GRGCYC 1 cut(s) 113
BauI CACGAG 1 cut(s) 1057
BbsI GAAGAC 1 cut(s) 146
Bbv12I GWGCWC 4 cut(s) 113, 407, 701, 993
BbvI GCAGC 5 cut(s) 55, 386, 406, 522, 969
BccI CCATC 5 cut(s) 424, 433, 590, 977, 1022
BceAI ACGGC 3 cut(s) 654, 862, 993
BciT130I CCWGG 1 cut(s) 680
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 2 cut(s) 540, 1113
BcoDI GTCTC 6 cut(s) 42, 214, 446, 555, 781, 935
BfaI CTAG 4 cut(s) 263, 686, 903, 1065
BfmI CTRYAG 3 cut(s) 465, 780, 948
BfuAI ACCTGC 1 cut(s) 458
BisI GCNGC 7 cut(s) 44, 375, 395, 536, 571, 642, 958
BlsI GCNGC 7 cut(s) 45, 376, 396, 537, 572, 643, 959
BmcAI AGTACT 1 cut(s) 205
Bme1390I CCNGG 3 cut(s) 540, 680, 1113
Bme18I GGWCC 3 cut(s) 68, 73, 815
BmgT120I GGNCC 5 cut(s) 68, 73, 650, 682, 815
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 3 cut(s) 540, 680, 1113
BmsI GCATC 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 146
Bpu14I TTCGAA 2 cut(s) 11, 333
BpuEI CTTGAG 2 cut(s) 144, 261
BpuMI CCSGG 2 cut(s) 540, 1113
BsaAI YACGTR 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 3 cut(s) 42, 555, 935
BsaJI CCNNGG 5 cut(s) 623, 679, 717, 1112, 1122
BsaWI WCCGGW 2 cut(s) 70, 1029
Bsc4I CCNNNNNNNGG 3 cut(s) 546, 629, 904
Bse1I ACTGG 1 cut(s) 564
Bse3DI GCAATG 2 cut(s) 960, 1134
BseAI TCCGGA 1 cut(s) 70
BseBI CCWGG 1 cut(s) 680
BseDI CCNNGG 5 cut(s) 623, 679, 717, 1112, 1122
BseGI GGATG 5 cut(s) 260, 298, 324, 435, 675
BseLI CCNNNNNNNGG 3 cut(s) 546, 629, 904
BseMI GCAATG 2 cut(s) 960, 1134
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 1 cut(s) 863
BseSI GKGCMC 1 cut(s) 407
BseXI GCAGC 5 cut(s) 55, 386, 406, 522, 969
Bsh1236I CGCG 2 cut(s) 422, 789
BshFI GGCC 3 cut(s) 651, 684, 1008
BsiHKAI GWGCWC 4 cut(s) 113, 407, 701, 993
BsiSI CCGG 7 cut(s) 71, 212, 540, 588, 818, 1030, 1112
BslI CCNNNNNNNGG 3 cut(s) 546, 629, 904
BsmAI GTCTC 6 cut(s) 42, 214, 446, 555, 781, 935
BsnI GGCC 3 cut(s) 651, 684, 1008
Bso31I GGTCTC 3 cut(s) 42, 555, 935
Bsp119I TTCGAA 2 cut(s) 11, 333
Bsp1286I GDGCHC 4 cut(s) 113, 407, 701, 993
Bsp13I TCCGGA 1 cut(s) 70
Bsp1407I TGTACA 1 cut(s) 1171
Bsp143I GATC 3 cut(s) 286, 295, 784
Bsp19I CCATGG 1 cut(s) 1122
Bsp68I TCGCGA 1 cut(s) 789
BspACI CCGC 5 cut(s) 41, 309, 570, 642, 794
BspANI GGCC 3 cut(s) 651, 684, 1008
BspEI TCCGGA 1 cut(s) 70
BspFNI CGCG 2 cut(s) 422, 789
BspLI GGNNCC 1 cut(s) 75
BspMAI CTGCAG 2 cut(s) 469, 784
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 1 cut(s) 281
BspT104I TTCGAA 2 cut(s) 11, 333
BspTNI GGTCTC 3 cut(s) 42, 555, 935
BsrBI CCGCTC 1 cut(s) 311
BsrDI GCAATG 2 cut(s) 960, 1134
BsrGI TGTACA 1 cut(s) 1171
BsrI ACTGG 1 cut(s) 564
BssECI CCNNGG 5 cut(s) 623, 679, 717, 1112, 1122
BssMI GATC 3 cut(s) 286, 295, 784
BssNI GRCGYC 1 cut(s) 53
BssSI CACGAG 1 cut(s) 1057
BssT1I CCWWGG 3 cut(s) 623, 717, 1122
Bst2BI CACGAG 1 cut(s) 1057
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 5 cut(s) 208, 366, 607, 931, 1024
Bst6I CTCTTC 2 cut(s) 734, 1068
BstACI GRCGYC 1 cut(s) 53
BstAPI GCANNNNNTGC 1 cut(s) 535
BstAUI TGTACA 1 cut(s) 1171
BstBAI YACGTR 1 cut(s) 128
BstBI TTCGAA 2 cut(s) 11, 333
BstC8I GCNNGC 4 cut(s) 309, 706, 837, 1040
BstDEI CTNAG 1 cut(s) 883
BstDSI CCRYGG 1 cut(s) 1122
BstF5I GGATG 5 cut(s) 260, 298, 324, 435, 675
BstFNI CGCG 2 cut(s) 422, 789
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 3 cut(s) 289, 298, 787
BstMAI GTCTC 6 cut(s) 42, 214, 446, 555, 781, 935
BstMBI GATC 3 cut(s) 286, 295, 784
BstMWI GCNNNNNNNGC 5 cut(s) 52, 535, 657, 666, 705
BstNI CCWGG 1 cut(s) 680
BstNSI RCATGY 2 cut(s) 418, 839
BstSCI CCNGG 3 cut(s) 538, 678, 1111
BstSFI CTRYAG 3 cut(s) 465, 780, 948
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 2 cut(s) 422, 789
BstV1I GCAGC 5 cut(s) 55, 386, 406, 522, 969
BstV2I GAAGAC 1 cut(s) 146
BsuRI GGCC 3 cut(s) 651, 684, 1008
BtgI CCRYGG 1 cut(s) 1122
BtgZI GCGATG 2 cut(s) 979, 1000
BtsCI GGATG 5 cut(s) 260, 298, 324, 435, 675
BtsIMutI CAGTG 1 cut(s) 1020
BtuMI TCGCGA 1 cut(s) 789
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 4 cut(s) 309, 706, 837, 1040
CaiI CAGNNNCTG 1 cut(s) 370
CfoI GCGC 1 cut(s) 349
Cfr13I GGNCC 5 cut(s) 68, 73, 650, 682, 815
CseI GACGC 1 cut(s) 61
Csp6I GTAC 8 cut(s) 143, 204, 418, 633, 767, 844, 1054, 1172
CviAII CATG 7 cut(s) 61, 304, 415, 638, 836, 1123, 1182
CviQI GTAC 8 cut(s) 143, 204, 418, 633, 767, 844, 1054, 1172
DdeI CTNAG 1 cut(s) 883
DpnI GATC 3 cut(s) 288, 297, 786
DpnII GATC 3 cut(s) 286, 295, 784
Eam1104I CTCTTC 2 cut(s) 734, 1068
EarI CTCTTC 2 cut(s) 734, 1068
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 3 cut(s) 623, 717, 1122
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 3 cut(s) 42, 555, 935
Eco47I GGWCC 3 cut(s) 68, 73, 815
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 2 cut(s) 165, 477
EcoICRI GAGCTC 1 cut(s) 111
EcoO109I RGGNCCY 2 cut(s) 650, 682
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 3 cut(s) 623, 717, 1122
EcoT22I ATGCAT 1 cut(s) 841
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 3 cut(s) 623, 717, 1122
FaeI CATG 7 cut(s) 64, 307, 418, 641, 839, 1126, 1185
FatI CATG 7 cut(s) 60, 303, 414, 637, 835, 1122, 1181
FauI CCCGC 1 cut(s) 316
FauNDI CATATG 1 cut(s) 187
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 7 cut(s) 44, 375, 395, 536, 571, 642, 958
FokI GGATG 5 cut(s) 267, 285, 311, 442, 662
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 7 cut(s) 44, 375, 395, 536, 571, 642, 958
FspBI CTAG 4 cut(s) 263, 686, 903, 1065
FspI TGCGCA 1 cut(s) 348
GlaI GCGC 1 cut(s) 348
GluI GCNGC 7 cut(s) 44, 375, 395, 536, 571, 642, 958
HaeIII GGCC 3 cut(s) 651, 684, 1008
HapII CCGG 7 cut(s) 71, 212, 540, 588, 818, 1030, 1112
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 349
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 7 cut(s) 64, 307, 418, 641, 839, 1126, 1185
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HindIII AAGCTT 1 cut(s) 706
HinfI GANTC 2 cut(s) 870, 890
HpaII CCGG 7 cut(s) 71, 212, 540, 588, 818, 1030, 1112
HphI GGTGA 3 cut(s) 445, 887, 1170
Hpy166II GTNNAC 3 cut(s) 405, 869, 1056
Hpy188I TCNGA 1 cut(s) 140
Hpy188III TCNNGA 4 cut(s) 71, 481, 554, 788
Hpy8I GTNNAC 3 cut(s) 405, 869, 1056
HpyAV CCTTC 5 cut(s) 7, 59, 86, 97, 806
HpyCH4III ACNGT 5 cut(s) 208, 366, 607, 931, 1024
HpyCH4IV ACGT 2 cut(s) 127, 477
HpyCH4V TGCA 5 cut(s) 405, 467, 782, 839, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 535, 657, 666, 705
HpyF3I CTNAG 1 cut(s) 883
HpySE526I ACGT 2 cut(s) 127, 477
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 7 cut(s) 64, 307, 418, 641, 839, 1126, 1185
HspAI GCGC 1 cut(s) 347
Kpn2I TCCGGA 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 3 cut(s) 286, 295, 784
LmnI GCTCC 1 cut(s) 1144
Lsp1109I GCAGC 5 cut(s) 55, 386, 406, 522, 969
LweI GCATC 1 cut(s) 245
MaeI CTAG 4 cut(s) 263, 686, 903, 1065
MaeII ACGT 2 cut(s) 127, 477
MalI GATC 3 cut(s) 288, 297, 786
MbiI CCGCTC 1 cut(s) 311
MboI GATC 3 cut(s) 286, 295, 784
MboII GAAGA 6 cut(s) 34, 101, 146, 448, 751, 1085
MhlI GDGCHC 4 cut(s) 113, 407, 701, 993
MluCI AATT 1 cut(s) 828
MlyI GAGTC 2 cut(s) 864, 884
Mph1103I ATGCAT 1 cut(s) 841
MroI TCCGGA 1 cut(s) 70
MseI TTAA 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 7 cut(s) 71, 212, 540, 588, 818, 1030, 1112
MspR9I CCNGG 3 cut(s) 540, 680, 1113
MvaI CCWGG 1 cut(s) 680
MvnI CGCG 2 cut(s) 422, 789
MwoI GCNNNNNNNGC 5 cut(s) 52, 535, 657, 666, 705
NciI CCSGG 2 cut(s) 540, 1113
NcoI CCATGG 1 cut(s) 1122
NdeI CATATG 1 cut(s) 187
NdeII GATC 3 cut(s) 286, 295, 784
NlaIII CATG 7 cut(s) 64, 307, 418, 641, 839, 1126, 1185
NlaIV GGNNCC 1 cut(s) 75
NruI TCGCGA 1 cut(s) 789
NsbI TGCGCA 1 cut(s) 348
NsiI ATGCAT 1 cut(s) 841
NspI RCATGY 2 cut(s) 418, 839
NspV TTCGAA 2 cut(s) 11, 333
PaeI GCATGC 1 cut(s) 839
PciI ACATGT 1 cut(s) 414
PkrI GCNGC 7 cut(s) 45, 376, 396, 537, 572, 643, 959
PleI GAGTC 2 cut(s) 864, 884
PpsI GAGTC 2 cut(s) 864, 884
Ppu21I YACGTR 1 cut(s) 128
PscI ACATGT 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 678
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 5 cut(s) 68, 73, 650, 682, 815
PstI CTGCAG 2 cut(s) 469, 784
PstNI CAGNNNCTG 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 293
RruI TCGCGA 1 cut(s) 789
RsaI GTAC 8 cut(s) 144, 205, 419, 634, 768, 845, 1055, 1173
RsaNI GTAC 8 cut(s) 143, 204, 418, 633, 767, 844, 1054, 1172
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 7 cut(s) 44, 375, 395, 536, 571, 642, 958
Sau3AI GATC 3 cut(s) 286, 295, 784
Sau96I GGNCC 5 cut(s) 68, 73, 650, 682, 815
ScaI AGTACT 1 cut(s) 205
SchI GAGTC 2 cut(s) 864, 884
ScrFI CCNGG 3 cut(s) 540, 680, 1113
SduI GDGCHC 4 cut(s) 113, 407, 701, 993
SfaNI GCATC 1 cut(s) 245
SfcI CTRYAG 3 cut(s) 465, 780, 948
SfuI TTCGAA 2 cut(s) 11, 333
SinI GGWCC 3 cut(s) 68, 73, 815
SmlI CTYRAG 2 cut(s) 159, 276
SmoI CTYRAG 2 cut(s) 159, 276
SphI GCATGC 1 cut(s) 839
Sse9I AATT 1 cut(s) 828
SsiI CCGC 5 cut(s) 41, 309, 570, 642, 794
SspMI CTAG 4 cut(s) 263, 686, 903, 1065
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 3 cut(s) 538, 678, 1111
StyI CCWWGG 3 cut(s) 623, 717, 1122
TaaI ACNGT 5 cut(s) 208, 366, 607, 931, 1024
TaiI ACGT 2 cut(s) 130, 480
TaqI TCGA 3 cut(s) 11, 333, 341
TasI AATT 1 cut(s) 828
TatI WGTACW 4 cut(s) 142, 203, 1053, 1171
TauI GCSGC 2 cut(s) 573, 644
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 1 cut(s) 1027
TseI GCWGC 5 cut(s) 43, 374, 394, 535, 957
TspDTI ATGAA 1 cut(s) 1123
TspRI CASTG 1 cut(s) 1027
VneI GTGCAC 1 cut(s) 403
VpaK11BI GGWCC 3 cut(s) 68, 73, 815
XceI RCATGY 2 cut(s) 418, 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
XspI CTAG 4 cut(s) 263, 686, 903, 1065
ZrmI AGTACT 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.