MD05G1238500.v1.1

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015065
Physical Location & Seq
Reverse (-)
36974043 .. 36976637
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1238500.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGCCTCAAAAGCAAGGAAGAAGCAGATATGGTGTCCACAGCCACTGACGCCGCTCATGGAAGGGCCGGACCCCGAAATGCAAGAGGAAGGTGGCAAAAAGGAGAGCTCTTGGGAAGTCATACGTGAATGGTTTAGGGCACAAAAGGGTGGCCCTCCTAGTCCCGGGAATAACTTATCGGCCTCAGGATATGGGAGCGGTAGTACTATTCCAGCCAAAAGACAAGACTTGAGGCTCTTGCTTGGTGTTTTAGGCTGTCCTCTTGCTCCAATTCCACAAGCCAATGACCCAGTTGATCCTCACCTCCTTCACATTAAGGACATCCCATTTGAAACTTCTATAGCGCATTACATTATAAAGCAGTACTTGGCAGCCACGGGGTGCTTGAAGCAACAGAAATGTAACAAAAACATGTATGCAAGTGGGCGTGTGAAAATGGTTTGCTGTGAAACTGAGGTTTCTGCAGGAAGAAATGTAAAGACTTCGGGAACAAGAAGTGGGGAAAGCGGGTGCTTTGTTCTTTGGCAAATGTTGCCTGGCATGTGGTCTTTGGAGTTGGTCGTTGGAGGCAACAAGGTGGTGGCTGGTAGTGATGGCAAAACGGTTTGGAGGCACACTCCTTGGCTTGGTATTCATGCAGCCAAAGGCCCCCAGCGCCCACTGCGTCGCCTCATACAGGGCCTAGATCCAAAGAGCACAGCAAGCTTGTTTTCCAAAGCACAATGCTTGGGAGAGGAAAGAATCGGAGACGATGATTGCTTTGTACTAAAAGTCTCTGCCGATCGAGCGGCGGTGATGGAAAGAAGTGAGGGTCCTGCAGAAGTAATCAGGCATGCATTGTATGGATATTTTTGTCAAAAGAGTGGTCTCCTCATATACTTAGAGGACTCACACCTCACCAGAGTTGAAACCCCAGAAAATGAAACTGTGTACTGGGAGACCACCATAGGAAGCAGCATTGCTGACTATAGGGATGTGGATGGTGTGCTCATTGCTCATCAGGGCCGCTCGATCGCCACAGTTTTCCGGTGTGGCGAGGTGTCGATGGAATACACAAGGACTAGGATGGAAGAAGCTTGGAGCATTGATGATGTTGTGTTTAATGTACCAGGGCTTTCCATGGACTATTTCATTGCTCCTGCTGATATATTTGATAGTACTGTACCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

392

Amino Acids

43.06

Weight (kDa)

6.28

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 138 - 380 2.2e-111 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015065)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79420
fragaria_vesca FvH4_3g14040
malus_domestica MD05G1238500.v1.1 MD10G1216800.v1.1
prunus_persica Prupe.4G127900_v2.0.a1
pyrus_communis pycom05g21460
rosa_chinensis RchiOBHm_Chr5g0023361
rosa_laevigata RLG00000032716
rosa_multiflora Rmu_sc0007223.1_g000005
rosa_roxburghii Rroxscaffold_1G00055980
rosa_rugosa Rorug05G0078600
rosa_samantha Rh5AG165500 Rh5BG164700 Rh5CG179600 Rh5DG166200
rosa_wichuraiana Rw5G014890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 356
AccBSI CCGCTC 4 cut(s) 55, 198, 788, 1008
AciI CCGC 6 cut(s) 53, 198, 507, 788, 791, 1006
AclWI GGATC 2 cut(s) 290, 680
AcyI GRCGYC 1 cut(s) 50
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 7 cut(s) 205, 365, 765, 932, 1107, 1159, 1164
AfiI CCNNNNNNNGG 3 cut(s) 164, 626, 676
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 3 cut(s) 332, 388, 908
AjnI CCWGG 2 cut(s) 535, 1108
AluBI AGCT 3 cut(s) 108, 705, 1076
AluI AGCT 3 cut(s) 108, 705, 1076
Alw21I GWGCWC 3 cut(s) 110, 698, 990
Alw26I GTCTC 4 cut(s) 741, 778, 872, 932
AlwI GGATC 2 cut(s) 290, 680
AlwNI CAGNNNCTG 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 7 cut(s) 3, 65, 151, 180, 646, 679, 1003
ApeKI GCWGC 3 cut(s) 371, 638, 954
ArsI GACNNNNNNTTYG 4 cut(s) 311, 343, 850, 882
AspLEI GCGC 2 cut(s) 346, 657
AspS9I GGNCC 7 cut(s) 65, 70, 152, 647, 679, 812, 1003
AsuC2I CCSGG 2 cut(s) 165, 166
AsuHPI GGTGA 4 cut(s) 293, 805, 889, 1161
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 2 cut(s) 70, 812
AxyI CCTNAGG 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 110
Bbv12I GWGCWC 3 cut(s) 110, 698, 990
BbvI GCAGC 3 cut(s) 383, 650, 966
BccI CCATC 6 cut(s) 587, 790, 974, 1039, 1060, 1174
BciT130I CCWGG 2 cut(s) 537, 1110
BcnI CCSGG 2 cut(s) 165, 166
BcoDI GTCTC 4 cut(s) 741, 778, 872, 932
BfaI CTAG 3 cut(s) 159, 683, 1062
BfmI CTRYAG 4 cut(s) 339, 462, 816, 967
BfoI RGCGCY 1 cut(s) 658
BisI GCNGC 6 cut(s) 53, 372, 639, 789, 955, 1006
BlsI GCNGC 6 cut(s) 54, 373, 640, 790, 956, 1007
BmcAI AGTACT 3 cut(s) 205, 365, 1159
Bme1390I CCNGG 4 cut(s) 165, 166, 537, 1110
Bme18I GGWCC 2 cut(s) 70, 812
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 7 cut(s) 65, 70, 152, 647, 679, 812, 1003
BmiI GGNNCC 3 cut(s) 72, 649, 813
BmrFI CCNGG 4 cut(s) 165, 166, 537, 1110
BmrI ACTGGG 2 cut(s) 284, 943
BmuI ACTGGG 2 cut(s) 284, 943
BplI GAGNNNNNCTC 2 cut(s) 601, 633
BpuEI CTTGAG 1 cut(s) 250
BpuMI CCSGG 2 cut(s) 165, 166
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 50
BsaI GGTCTC 2 cut(s) 872, 932
BsaJI CCNNGG 5 cut(s) 164, 375, 620, 1109, 1119
BsaWI WCCGGW 1 cut(s) 1026
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 626, 676
Bse1I ACTGG 2 cut(s) 290, 938
Bse21I CCTNAGG 1 cut(s) 184
Bse3DI GCAATG 3 cut(s) 957, 990, 1131
BseBI CCWGG 2 cut(s) 537, 1110
BseDI CCNNGG 5 cut(s) 164, 375, 620, 1109, 1119
BseGI GGATG 4 cut(s) 321, 979, 985, 1071
BseLI CCNNNNNNNGG 3 cut(s) 164, 626, 676
BseMI GCAATG 3 cut(s) 957, 990, 1131
BseMII CTCAG 2 cut(s) 198, 444
BseNI ACTGG 2 cut(s) 290, 938
BseRI GAGGAG 1 cut(s) 860
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 3 cut(s) 383, 650, 966
BseYI CCCAGC 1 cut(s) 651
Bsh1285I CGRYCG 2 cut(s) 784, 1014
BshFI GGCC 7 cut(s) 5, 67, 153, 182, 648, 681, 1005
BsiEI CGRYCG 2 cut(s) 784, 1014
BsiHKAI GWGCWC 3 cut(s) 110, 698, 990
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 3 cut(s) 68, 165, 1027
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 3 cut(s) 164, 626, 676
BsmAI GTCTC 4 cut(s) 741, 778, 872, 932
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 147
BsnI GGCC 7 cut(s) 5, 67, 153, 182, 648, 681, 1005
Bso31I GGTCTC 2 cut(s) 872, 932
BsoBI CYCGRG 1 cut(s) 164
Bsp1286I GDGCHC 4 cut(s) 110, 142, 698, 990
Bsp143I GATC 4 cut(s) 295, 685, 781, 1011
Bsp19I CCATGG 1 cut(s) 1119
BspACI CCGC 6 cut(s) 53, 198, 507, 788, 791, 1006
BspANI GGCC 7 cut(s) 5, 67, 153, 182, 648, 681, 1005
BspCNI CTCAG 2 cut(s) 197, 445
BspLI GGNNCC 3 cut(s) 72, 649, 813
BspMAI CTGCAG 2 cut(s) 466, 820
BspPI GGATC 2 cut(s) 290, 680
BspTNI GGTCTC 2 cut(s) 872, 932
BsrBI CCGCTC 4 cut(s) 55, 198, 788, 1008
BsrDI GCAATG 3 cut(s) 957, 990, 1131
BsrI ACTGG 2 cut(s) 290, 938
BssECI CCNNGG 5 cut(s) 164, 375, 620, 1109, 1119
BssMI GATC 4 cut(s) 295, 685, 781, 1011
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 2 cut(s) 620, 1119
Bst2UI CCWGG 2 cut(s) 537, 1110
Bst4CI ACNGT 4 cut(s) 604, 928, 1021, 1162
BstACI GRCGYC 1 cut(s) 50
BstAPI GCANNNNNTGC 1 cut(s) 532
BstBAI YACGTR 1 cut(s) 125
BstC8I GCNNGC 2 cut(s) 703, 834
BstDEI CTNAG 3 cut(s) 184, 453, 880
BstDSI CCRYGG 2 cut(s) 375, 1119
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 4 cut(s) 321, 979, 985, 1071
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 2 cut(s) 346, 657
BstKTI GATC 4 cut(s) 298, 688, 784, 1014
BstMAI GTCTC 4 cut(s) 741, 778, 872, 932
BstMBI GATC 4 cut(s) 295, 685, 781, 1011
BstMCI CGRYCG 2 cut(s) 784, 1014
BstMWI GCNNNNNNNGC 7 cut(s) 11, 49, 532, 654, 661, 702, 785
BstNI CCWGG 2 cut(s) 537, 1110
BstNSI RCATGY 3 cut(s) 415, 544, 836
BstSCI CCNGG 4 cut(s) 163, 164, 535, 1108
BstSFI CTRYAG 4 cut(s) 339, 462, 816, 967
BstSLI GKGCMC 1 cut(s) 142
BstV1I GCAGC 3 cut(s) 383, 650, 966
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
Bsu36I CCTNAGG 1 cut(s) 184
BsuRI GGCC 7 cut(s) 5, 67, 153, 182, 648, 681, 1005
BtgI CCRYGG 2 cut(s) 375, 1119
BtsCI GGATG 4 cut(s) 321, 979, 985, 1071
BtsI GCAGTG 1 cut(s) 659
BtsIMutI CAGTG 2 cut(s) 44, 659
Cac8I GCNNGC 2 cut(s) 703, 834
CaiI CAGNNNCTG 1 cut(s) 46
CfoI GCGC 2 cut(s) 346, 657
Cfr13I GGNCC 7 cut(s) 65, 70, 152, 647, 679, 812, 1003
Cfr9I CCCGGG 1 cut(s) 164
CseI GACGC 2 cut(s) 58, 653
Csp6I GTAC 7 cut(s) 204, 364, 764, 931, 1106, 1158, 1163
CviAII CATG 7 cut(s) 58, 412, 541, 635, 833, 1120, 1173
CviQI GTAC 7 cut(s) 204, 364, 764, 931, 1106, 1158, 1163
DdeI CTNAG 3 cut(s) 184, 453, 880
DpnI GATC 4 cut(s) 297, 687, 783, 1013
DpnII GATC 4 cut(s) 295, 685, 781, 1011
DraIII CACNNNGTG 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 620, 1119
Eco24I GRGCYC 1 cut(s) 110
Eco31I GGTCTC 2 cut(s) 872, 932
Eco47I GGWCC 2 cut(s) 70, 812
Eco53kI GAGCTC 1 cut(s) 108
Eco81I CCTNAGG 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 108
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO109I RGGNCCY 3 cut(s) 647, 679, 812
EcoRII CCWGG 2 cut(s) 535, 1108
EcoT14I CCWWGG 2 cut(s) 620, 1119
EcoT22I ATGCAT 1 cut(s) 838
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 2 cut(s) 620, 1119
Esp3I CGTCTC 1 cut(s) 741
FaeI CATG 7 cut(s) 61, 415, 544, 638, 836, 1123, 1176
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 1 cut(s) 147
FatI CATG 7 cut(s) 57, 411, 540, 634, 832, 1119, 1172
FauI CCCGC 1 cut(s) 500
Fnu4HI GCNGC 6 cut(s) 53, 372, 639, 789, 955, 1006
FokI GGATG 4 cut(s) 308, 986, 992, 1078
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 6 cut(s) 53, 372, 639, 789, 955, 1006
FspBI CTAG 3 cut(s) 159, 683, 1062
GlaI GCGC 2 cut(s) 345, 656
GluI GCNGC 6 cut(s) 53, 372, 639, 789, 955, 1006
GsaI CCCAGC 1 cut(s) 655
HaeII RGCGCY 1 cut(s) 658
HaeIII GGCC 7 cut(s) 5, 67, 153, 182, 648, 681, 1005
HapII CCGG 3 cut(s) 68, 165, 1027
HgaI GACGC 2 cut(s) 58, 653
HhaI GCGC 2 cut(s) 346, 657
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 7 cut(s) 61, 415, 544, 638, 836, 1123, 1176
Hin6I GCGC 2 cut(s) 344, 655
HinP1I GCGC 2 cut(s) 344, 655
HindIII AAGCTT 2 cut(s) 703, 1074
HinfI GANTC 2 cut(s) 741, 887
HpaII CCGG 3 cut(s) 68, 165, 1027
HphI GGTGA 4 cut(s) 293, 805, 889, 1161
Hpy166II GTNNAC 2 cut(s) 38, 931
Hpy188I TCNGA 1 cut(s) 746
Hpy188III TCNNGA 2 cut(s) 186, 486
Hpy8I GTNNAC 2 cut(s) 38, 931
Hpy99I CGWCG 1 cut(s) 669
HpyAV CCTTC 3 cut(s) 56, 83, 317
HpyCH4III ACNGT 4 cut(s) 604, 928, 1021, 1162
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 6 cut(s) 82, 419, 464, 638, 818, 836
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 49, 532, 654, 661, 702, 785
HpyF3I CTNAG 3 cut(s) 184, 453, 880
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 7 cut(s) 61, 415, 544, 638, 836, 1123, 1176
HspAI GCGC 2 cut(s) 344, 655
Kzo9I GATC 4 cut(s) 295, 685, 781, 1011
LmnI GCTCC 4 cut(s) 195, 271, 1080, 1141
Lsp1109I GCAGC 3 cut(s) 383, 650, 966
MaeI CTAG 3 cut(s) 159, 683, 1062
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 1 cut(s) 401
MalI GATC 4 cut(s) 297, 687, 783, 1013
MbiI CCGCTC 4 cut(s) 55, 198, 788, 1008
MboI GATC 4 cut(s) 295, 685, 781, 1011
MboII GAAGA 3 cut(s) 31, 480, 1082
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 4 cut(s) 110, 142, 698, 990
MluCI AATT 1 cut(s) 270
MlyI GAGTC 1 cut(s) 881
MmeI TCCRAC 1 cut(s) 544
Mph1103I ATGCAT 1 cut(s) 838
MseI TTAA 2 cut(s) 315, 1101
MslI CAYNNNNRTG 1 cut(s) 1171
MspI CCGG 3 cut(s) 68, 165, 1027
MspR9I CCNGG 4 cut(s) 165, 166, 537, 1110
MvaI CCWGG 2 cut(s) 537, 1110
MwoI GCNNNNNNNGC 7 cut(s) 11, 49, 532, 654, 661, 702, 785
NciI CCSGG 2 cut(s) 165, 166
NcoI CCATGG 1 cut(s) 1119
NdeII GATC 4 cut(s) 295, 685, 781, 1011
NlaIII CATG 7 cut(s) 61, 415, 544, 638, 836, 1123, 1176
NlaIV GGNNCC 3 cut(s) 72, 649, 813
NsiI ATGCAT 1 cut(s) 838
NspI RCATGY 3 cut(s) 415, 544, 836
PaeI GCATGC 1 cut(s) 836
PciI ACATGT 1 cut(s) 411
PfeI GAWTC 1 cut(s) 741
PkrI GCNGC 6 cut(s) 54, 373, 640, 790, 956, 1007
Ple19I CGATCG 2 cut(s) 784, 1014
PleI GAGTC 1 cut(s) 881
PpsI GAGTC 1 cut(s) 881
Ppu21I YACGTR 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 812
PscI ACATGT 1 cut(s) 411
PsiI TTATAA 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 812
Psp6I CCWGG 2 cut(s) 535, 1108
PspFI CCCAGC 1 cut(s) 651
PspGI CCWGG 2 cut(s) 535, 1108
PspN4I GGNNCC 3 cut(s) 72, 649, 813
PspPI GGNCC 7 cut(s) 65, 70, 152, 647, 679, 812, 1003
PspPPI RGGWCCY 1 cut(s) 812
PstI CTGCAG 2 cut(s) 466, 820
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 685
PvuI CGATCG 2 cut(s) 784, 1014
RsaI GTAC 7 cut(s) 205, 365, 765, 932, 1107, 1159, 1164
RsaNI GTAC 7 cut(s) 204, 364, 764, 931, 1106, 1158, 1163
RseI CAYNNNNRTG 1 cut(s) 1171
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 2 cut(s) 315, 1101
SatI GCNGC 6 cut(s) 53, 372, 639, 789, 955, 1006
Sau3AI GATC 4 cut(s) 295, 685, 781, 1011
Sau96I GGNCC 7 cut(s) 65, 70, 152, 647, 679, 812, 1003
ScaI AGTACT 3 cut(s) 205, 365, 1159
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 4 cut(s) 165, 166, 537, 1110
SduI GDGCHC 4 cut(s) 110, 142, 698, 990
SfcI CTRYAG 4 cut(s) 339, 462, 816, 967
SinI GGWCC 2 cut(s) 70, 812
SmaI CCCGGG 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 1171
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
SphI GCATGC 1 cut(s) 836
Sse9I AATT 1 cut(s) 270
SsiI CCGC 6 cut(s) 53, 198, 507, 788, 791, 1006
SspMI CTAG 3 cut(s) 159, 683, 1062
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 4 cut(s) 163, 164, 535, 1108
StyI CCWWGG 2 cut(s) 620, 1119
TaaI ACNGT 4 cut(s) 604, 928, 1021, 1162
TaiI ACGT 1 cut(s) 127
TaqI TCGA 3 cut(s) 784, 1010, 1043
TasI AATT 1 cut(s) 270
TatI WGTACW 5 cut(s) 203, 363, 763, 930, 1157
TauI GCSGC 3 cut(s) 55, 791, 1008
TfiI GAWTC 1 cut(s) 741
Tru1I TTAA 2 cut(s) 315, 1101
Tru9I TTAA 2 cut(s) 315, 1101
TscAI CASTG 2 cut(s) 51, 666
TseI GCWGC 3 cut(s) 371, 638, 954
TspDTI ATGAA 3 cut(s) 623, 936, 1120
TspMI CCCGGG 1 cut(s) 164
TspRI CASTG 2 cut(s) 51, 666
VpaK11BI GGWCC 2 cut(s) 70, 812
XagI CCTNNNNNAGG 1 cut(s) 674
XceI RCATGY 3 cut(s) 415, 544, 836
XmaI CCCGGG 1 cut(s) 164
XspI CTAG 3 cut(s) 159, 683, 1062
ZrmI AGTACT 3 cut(s) 205, 365, 1159
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.