Rh5AG165500

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0015065
Physical Location & Seq
Forward (+)
17811482 .. 17814258
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG165500.1

Sequence Viewer

Length: 1182 bp
ATGATGGGTTCAAAGGCGAGGAAGAAGCAGATGTGGTCTCCGCAGCCATTGACGCCACTCATGGAAGGTCCAGACCCAGAAATGCAAGAAGAAGGAGGTAAGAAGGAAAGCTCTTGGGAAGCCATACGTGAATGGTTCAGAGCACAAAAGGGTCTTCCTCAAGGAAGTAACTTATCAACTTCGGCATATGGAAGTAATGGTAGTACCATTCCGGCAAAGAGACAAGATTTGAGGCTCTTGCTTGGTGTGTTGGGCTGTCCTTTAGCTCCAATTCCTCAAGCCAGTGAGCCAGTTGATCATCCCATGCCCCACATCAAAGACATCCCATTTGAAACTTCGACGGCGCACTACATTATACAACAGTATCTGGCAGCAAGCGGGTGCTTGAAGCAGCAGAAGTGCAACAAGAACATGTATGCGAGTGGGATGGTGAAGATGGTCTGCTGTGAGACTGAGGTTTCTGCAGGTAGAAATGTGAAGACTTTGGGAACAAGAAGTGGGGAAAGTGGGTGCTTTGTTCTTTGGCAAATGTTACCCGGCATGTGGTCTCTGGAATTGGTGGTCGGCGGCAACAAGGTGGTGGCCGGAAGTGATGGAAAGACTGTGTGGAGGCACACCCCTTGGCTTGGTACTCATGCTGCCAAAGGCCCTCAACGCCCATTACGCCGCATCATCCAGGGCCTAGACCCAAAGAGCACAGCAAGCTTGTTTGCCAAGGCACAATGCTTGGGGGAGAAGAGGATTGGCGACGATGATTGCTTTGTACTAAAAGTTTCTGCAGACCGTGAGGCTGTAATGGAACGAAGCGAAGGTCCTGCAGAGGTAATTAGGCATGCATTGTACGGCTATTTCTGTCAAAAGAGTGGACTCCTCATCTACTTAGAGGACTCGCACCTCACTAGGGTTGAAACCCCAGAAAACGAAACTGTGTATTGGGAGACCACCATAGGAAGCAGCATTGCAGATTACAGAGATGTTGATGGTGTGCTCATCGCTCATCAAGGCCGTTCCATCGCCACCGTTTTCCGGTGTGGCGAGCTATCAATGGAGTATACGAGGACTAGAATGGAAGAGGTTTGGAACATTGATGATGTTGTGTTCAATGTGCCGGGGCTTTCCATGGACTATTTCATTGCTCCTGCTGATATATATGATAGTAGTAGTGCTGTACAATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

393

Amino Acids

43.44

Weight (kDa)

6.11

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 138 - 380 1.3e-111 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015065)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79420
fragaria_vesca FvH4_3g14040
malus_domestica MD05G1238500.v1.1 MD10G1216800.v1.1
prunus_persica Prupe.4G127900_v2.0.a1
pyrus_communis pycom05g21460
rosa_chinensis RchiOBHm_Chr5g0023361
rosa_laevigata RLG00000032716
rosa_multiflora Rmu_sc0007223.1_g000005
rosa_roxburghii Rroxscaffold_1G00055980
rosa_rugosa Rorug05G0078600
rosa_samantha Rh5AG165500 Rh5BG164700 Rh5CG179600 Rh5DG166200
rosa_wichuraiana Rw5G014890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
AccI GTMKAC 1 cut(s) 1052
AciI CCGC 4 cut(s) 41, 378, 567, 667
AcoI YGGCCR 1 cut(s) 582
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 5 cut(s) 205, 631, 765, 842, 1170
AfiI CCNNNNNNNGG 4 cut(s) 543, 626, 901, 1026
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 5 cut(s) 12, 332, 388, 908, 1102
AjnI CCWGG 1 cut(s) 675
AluBI AGCT 4 cut(s) 111, 266, 705, 1039
AluI AGCT 4 cut(s) 111, 266, 705, 1039
Alw21I GWGCWC 3 cut(s) 145, 698, 990
Alw26I GTCTC 5 cut(s) 42, 214, 443, 552, 932
AlwNI CAGNNNCTG 1 cut(s) 367
AoxI GGCC 4 cut(s) 582, 646, 679, 1003
ApeKI GCWGC 5 cut(s) 43, 371, 391, 638, 954
ArsI GACNNNNNNTTYG 4 cut(s) 311, 343, 796, 828
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 4 cut(s) 68, 647, 679, 812
AsuC2I CCSGG 2 cut(s) 537, 1110
AsuHPI GGTGA 2 cut(s) 442, 1167
AvaII GGWCC 2 cut(s) 68, 812
BaeI ACNNNNGTAYC 2 cut(s) 347, 380
BbsI GAAGAC 2 cut(s) 146, 485
Bbv12I GWGCWC 3 cut(s) 145, 698, 990
BbvI GCAGC 5 cut(s) 55, 383, 403, 625, 966
BccI CCATC 5 cut(s) 421, 430, 587, 974, 1019
BceAI ACGGC 3 cut(s) 357, 859, 990
BcgI CGANNNNNNTGC 2 cut(s) 797, 831
BciT130I CCWGG 1 cut(s) 677
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 2 cut(s) 537, 1110
BcoDI GTCTC 5 cut(s) 42, 214, 443, 552, 932
BfaI CTAG 3 cut(s) 683, 900, 1062
BfmI CTRYAG 3 cut(s) 462, 777, 816
BfuAI ACCTGC 1 cut(s) 455
BisI GCNGC 7 cut(s) 44, 372, 392, 568, 639, 667, 955
BlsI GCNGC 7 cut(s) 45, 373, 393, 569, 640, 668, 956
Bme1390I CCNGG 3 cut(s) 537, 677, 1110
Bme18I GGWCC 2 cut(s) 68, 812
BmgT120I GGNCC 4 cut(s) 68, 647, 679, 812
BmrFI CCNGG 3 cut(s) 537, 677, 1110
BmsI GCATC 1 cut(s) 678
BpiI GAAGAC 2 cut(s) 146, 485
BpuEI CTTGAG 2 cut(s) 144, 261
BpuMI CCSGG 2 cut(s) 537, 1110
BsaAI YACGTR 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 3 cut(s) 42, 552, 932
BsaJI CCNNGG 5 cut(s) 620, 676, 714, 1109, 1119
BsaWI WCCGGW 1 cut(s) 1026
Bsc4I CCNNNNNNNGG 4 cut(s) 543, 626, 901, 1026
Bse1I ACTGG 2 cut(s) 282, 290
Bse3DI GCAATG 2 cut(s) 957, 1131
BseBI CCWGG 1 cut(s) 677
BseDI CCNNGG 5 cut(s) 620, 676, 714, 1109, 1119
BseGI GGATG 4 cut(s) 298, 321, 432, 672
BseLI CCNNNNNNNGG 4 cut(s) 543, 626, 901, 1026
BseMI GCAATG 2 cut(s) 957, 1131
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 2 cut(s) 282, 290
BseRI GAGGAG 1 cut(s) 860
BseXI GCAGC 5 cut(s) 55, 383, 403, 625, 966
BshFI GGCC 4 cut(s) 584, 648, 681, 1005
BsiHKAI GWGCWC 3 cut(s) 145, 698, 990
BsiSI CCGG 5 cut(s) 212, 537, 585, 1027, 1109
BslI CCNNNNNNNGG 4 cut(s) 543, 626, 901, 1026
BsmAI GTCTC 5 cut(s) 42, 214, 443, 552, 932
BsnI GGCC 4 cut(s) 584, 648, 681, 1005
Bso31I GGTCTC 3 cut(s) 42, 552, 932
Bsp1286I GDGCHC 3 cut(s) 145, 698, 990
Bsp1407I TGTACA 1 cut(s) 1168
Bsp143I GATC 1 cut(s) 295
Bsp19I CCATGG 1 cut(s) 1119
BspACI CCGC 4 cut(s) 41, 378, 567, 667
BspANI GGCC 4 cut(s) 584, 648, 681, 1005
BspCNI CTCAG 1 cut(s) 445
BspMAI CTGCAG 3 cut(s) 466, 781, 820
BspMI ACCTGC 1 cut(s) 455
BspTNI GGTCTC 3 cut(s) 42, 552, 932
BsrDI GCAATG 2 cut(s) 957, 1131
BsrGI TGTACA 1 cut(s) 1168
BsrI ACTGG 2 cut(s) 282, 290
BssECI CCNNGG 5 cut(s) 620, 676, 714, 1109, 1119
BssMI GATC 1 cut(s) 295
BssNAI GTATAC 1 cut(s) 1053
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 3 cut(s) 620, 714, 1119
Bst1107I GTATAC 1 cut(s) 1053
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 5 cut(s) 363, 604, 785, 928, 1021
Bst6I CTCTTC 2 cut(s) 731, 1065
BstACI GRCGYC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 1168
BstBAI YACGTR 1 cut(s) 128
BstC8I GCNNGC 4 cut(s) 376, 703, 834, 1037
BstDEI CTNAG 2 cut(s) 453, 880
BstDSI CCRYGG 1 cut(s) 1119
BstF5I GGATG 4 cut(s) 298, 321, 432, 672
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 1 cut(s) 298
BstMAI GTCTC 5 cut(s) 42, 214, 443, 552, 932
BstMBI GATC 1 cut(s) 295
BstMWI GCNNNNNNNGC 4 cut(s) 52, 654, 663, 702
BstNI CCWGG 1 cut(s) 677
BstNSI RCATGY 3 cut(s) 415, 544, 836
BstSCI CCNGG 3 cut(s) 535, 675, 1108
BstSFI CTRYAG 3 cut(s) 462, 777, 816
BstV1I GCAGC 5 cut(s) 55, 383, 403, 625, 966
BstV2I GAAGAC 2 cut(s) 146, 485
BstZ17I GTATAC 1 cut(s) 1053
BsuRI GGCC 4 cut(s) 584, 648, 681, 1005
BtgI CCRYGG 1 cut(s) 1119
BtgZI GCGATG 2 cut(s) 976, 997
BtsCI GGATG 4 cut(s) 298, 321, 432, 672
BtsIMutI CAGTG 1 cut(s) 289
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 4 cut(s) 376, 703, 834, 1037
CaiI CAGNNNCTG 1 cut(s) 367
CfoI GCGC 1 cut(s) 346
Cfr13I GGNCC 4 cut(s) 68, 647, 679, 812
CseI GACGC 1 cut(s) 61
Csp6I GTAC 5 cut(s) 204, 630, 764, 841, 1169
CviAII CATG 8 cut(s) 61, 304, 412, 541, 635, 833, 1120, 1179
CviQI GTAC 5 cut(s) 204, 630, 764, 841, 1169
DdeI CTNAG 2 cut(s) 453, 880
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 2 cut(s) 731, 1065
EarI CTCTTC 2 cut(s) 731, 1065
Eco130I CCWWGG 3 cut(s) 620, 714, 1119
Eco31I GGTCTC 3 cut(s) 42, 552, 932
Eco47I GGWCC 2 cut(s) 68, 812
EcoO109I RGGNCCY 3 cut(s) 647, 679, 812
EcoRII CCWGG 1 cut(s) 675
EcoT14I CCWWGG 3 cut(s) 620, 714, 1119
EcoT22I ATGCAT 1 cut(s) 838
ErhI CCWWGG 3 cut(s) 620, 714, 1119
FaeI CATG 8 cut(s) 64, 307, 415, 544, 638, 836, 1123, 1182
FatI CATG 8 cut(s) 60, 303, 411, 540, 634, 832, 1119, 1178
FauI CCCGC 1 cut(s) 371
FauNDI CATATG 1 cut(s) 187
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 1 cut(s) 1052
Fnu4HI GCNGC 7 cut(s) 44, 372, 392, 568, 639, 667, 955
FokI GGATG 4 cut(s) 285, 308, 439, 659
Fsp4HI GCNGC 7 cut(s) 44, 372, 392, 568, 639, 667, 955
FspBI CTAG 3 cut(s) 683, 900, 1062
GlaI GCGC 1 cut(s) 345
GluI GCNGC 7 cut(s) 44, 372, 392, 568, 639, 667, 955
HaeIII GGCC 4 cut(s) 584, 648, 681, 1005
HapII CCGG 5 cut(s) 212, 537, 585, 1027, 1109
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 346
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 8 cut(s) 64, 307, 415, 544, 638, 836, 1123, 1182
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HindIII AAGCTT 1 cut(s) 703
HinfI GANTC 2 cut(s) 867, 887
HpaII CCGG 5 cut(s) 212, 537, 585, 1027, 1109
HphI GGTGA 2 cut(s) 442, 1167
Hpy166II GTNNAC 2 cut(s) 866, 1053
Hpy188I TCNGA 1 cut(s) 140
Hpy188III TCNNGA 2 cut(s) 71, 551
Hpy8I GTNNAC 2 cut(s) 866, 1053
Hpy99I CGWCG 2 cut(s) 343, 752
HpyAV CCTTC 4 cut(s) 59, 86, 97, 803
HpyCH4III ACNGT 5 cut(s) 363, 604, 785, 928, 1021
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 7 cut(s) 85, 402, 464, 779, 818, 836, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 654, 663, 702
HpyF3I CTNAG 2 cut(s) 453, 880
HpySE526I ACGT 1 cut(s) 127
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 8 cut(s) 64, 307, 415, 544, 638, 836, 1123, 1182
HspAI GCGC 1 cut(s) 344
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 1 cut(s) 295
LmnI GCTCC 2 cut(s) 271, 1141
Lsp1109I GCAGC 5 cut(s) 55, 383, 403, 625, 966
LweI GCATC 1 cut(s) 678
MaeI CTAG 3 cut(s) 683, 900, 1062
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 2 cut(s) 167, 531
MalI GATC 1 cut(s) 297
MboI GATC 1 cut(s) 295
MboII GAAGA 7 cut(s) 34, 101, 146, 445, 490, 748, 1082
MhlI GDGCHC 3 cut(s) 145, 698, 990
MluCI AATT 3 cut(s) 270, 554, 825
MlyI GAGTC 2 cut(s) 861, 881
Mph1103I ATGCAT 1 cut(s) 838
MspI CCGG 5 cut(s) 212, 537, 585, 1027, 1109
MspR9I CCNGG 3 cut(s) 537, 677, 1110
MvaI CCWGG 1 cut(s) 677
MwoI GCNNNNNNNGC 4 cut(s) 52, 654, 663, 702
NciI CCSGG 2 cut(s) 537, 1110
NcoI CCATGG 1 cut(s) 1119
NdeI CATATG 1 cut(s) 187
NdeII GATC 1 cut(s) 295
NlaIII CATG 8 cut(s) 64, 307, 415, 544, 638, 836, 1123, 1182
NsiI ATGCAT 1 cut(s) 838
NspI RCATGY 3 cut(s) 415, 544, 836
PaeI GCATGC 1 cut(s) 836
PciI ACATGT 1 cut(s) 411
PkrI GCNGC 7 cut(s) 45, 373, 393, 569, 640, 668, 956
PleI GAGTC 2 cut(s) 861, 881
PpsI GAGTC 2 cut(s) 861, 881
Ppu21I YACGTR 1 cut(s) 128
PpuMI RGGWCCY 1 cut(s) 812
PscI ACATGT 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 812
Psp6I CCWGG 1 cut(s) 675
PspGI CCWGG 1 cut(s) 675
PspPI GGNCC 4 cut(s) 68, 647, 679, 812
PspPPI RGGWCCY 1 cut(s) 812
PstI CTGCAG 3 cut(s) 466, 781, 820
PstNI CAGNNNCTG 1 cut(s) 367
RsaI GTAC 5 cut(s) 205, 631, 765, 842, 1170
RsaNI GTAC 5 cut(s) 204, 630, 764, 841, 1169
SatI GCNGC 7 cut(s) 44, 372, 392, 568, 639, 667, 955
Sau3AI GATC 1 cut(s) 295
Sau96I GGNCC 4 cut(s) 68, 647, 679, 812
SchI GAGTC 2 cut(s) 861, 881
ScrFI CCNGG 3 cut(s) 537, 677, 1110
SduI GDGCHC 3 cut(s) 145, 698, 990
SfaNI GCATC 1 cut(s) 678
SfcI CTRYAG 3 cut(s) 462, 777, 816
SinI GGWCC 2 cut(s) 68, 812
SmlI CTYRAG 2 cut(s) 159, 276
SmoI CTYRAG 2 cut(s) 159, 276
SphI GCATGC 1 cut(s) 836
Sse9I AATT 3 cut(s) 270, 554, 825
SsiI CCGC 4 cut(s) 41, 378, 567, 667
SspMI CTAG 3 cut(s) 683, 900, 1062
StyD4I CCNGG 3 cut(s) 535, 675, 1108
StyI CCWWGG 3 cut(s) 620, 714, 1119
TaaI ACNGT 5 cut(s) 363, 604, 785, 928, 1021
TaiI ACGT 1 cut(s) 130
TaqI TCGA 1 cut(s) 338
TasI AATT 3 cut(s) 270, 554, 825
TatI WGTACW 2 cut(s) 763, 1168
TauI GCSGC 2 cut(s) 570, 669
TscAI CASTG 1 cut(s) 289
TseI GCWGC 5 cut(s) 43, 371, 391, 638, 954
TspDTI ATGAA 1 cut(s) 1120
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 2 cut(s) 68, 812
XceI RCATGY 3 cut(s) 415, 544, 836
XmiI GTMKAC 1 cut(s) 1052
XspI CTAG 3 cut(s) 683, 900, 1062
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.