MD10G1216800.v1.1

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015065
Physical Location & Seq
Reverse (-)
31531950 .. 31534625
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1216800.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGCTTCTAAAGCAAGGAAGAAGCAGATATGGTGTCCACAGCCACTGACGCCACTCATGGAAGGGCCAGACCCGGAAATGCAAGAGGAAGGTGGGAAAAAGGAGAGCTCTTGGGAAGTCATTCGTGAATGGTTTAGGGCACAAAAGGGTGGTCCTCCTAGTCCCGGGACTAATTTGTCAGCCTCGGGATATGGCAGTGGTGGTACTATTCCAGCCAAAGGACAAGACTTGAGGCTCTTGCTTGGTGTTTTAGGCTGTCCTCTTGCTCCAATCCCGCAAACCAATGACCCAATTGATCATCACCTCCTTCACATTAAAGACATCCCATTTGAAACTTCTATAGCGCATTACATTATACAGCAGTATTTGGCAGCCACGGGGTGCTTGAAGCAACAGAAGTGTAACAAAAACATGTATGCAAGTGGGAGCGTGAAGATGGTTTGCTGTGAAACGGAGGTTTCTGCAGGAAGAAATGTGAAGACTTTGGGAACAAGAAGTGGGGAAAACGGGTGCTTTGTTCTTTGGCAAATGTTGCCTGGAATGTGGTCTTTGGAGTTGGTCGTTGGAGGCATCAAGGTGGTGGCTGGTAGTGATGGCAAGACGGTTTGGAGGCACACCCCTTGGCTGGGTACTCATGCAGCCAAAGGCCCCCAGCGCCCGTTGCGCCGCCTCATACAGGGCCTAGATCCGAAAAGCACAGCAAGCTTGTTCGCCAAGGCACAGTGCTTGGGAGAGAAAAGAATCGGAGACGATGATTGCTTTGTACTAAAAGTCTCTGCCGATCGAGCGGCTGTGATGGAAAGAAGTGAGGGTCCTGCAGAGGTGATCCGACATGCGTTGTATGGATATTTTTGCCAAAAGAGTGGCCTCCTCATTTATTTAGAGGACTCACACCTCACCAGAGTTGAAACCCCAGAAAGTGAAACTGTGTACTGGGAGACCACCATAGGAAGCAGCATTGCTGACTATAGGGATGTCGATGGTGTGCTCATTGCTCATCAGGGCCGCTCAATCGCCACAGTTTTCCGGTGTGGTGAGGTGTCGATGGAGTACACAAGGACTAGGATGGAAGAAGCTTGGAGCATTGATGATGTTGTGTTTAATGTACCAGGGCTTTCCATGGACTATTTCATTGCTCCTGCCGATGTATTTGATAGTACTCTACCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

392

Amino Acids

42.93

Weight (kDa)

6.17

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 138 - 380 8.7e-112 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015065)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79420
fragaria_vesca FvH4_3g14040
malus_domestica MD05G1238500.v1.1 MD10G1216800.v1.1
prunus_persica Prupe.4G127900_v2.0.a1
pyrus_communis pycom05g21460
rosa_chinensis RchiOBHm_Chr5g0023361
rosa_laevigata RLG00000032716
rosa_multiflora Rmu_sc0007223.1_g000005
rosa_roxburghii Rroxscaffold_1G00055980
rosa_rugosa Rorug05G0078600
rosa_samantha Rh5AG165500 Rh5BG164700 Rh5CG179600 Rh5DG166200
rosa_wichuraiana Rw5G014890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 175
AccBSI CCGCTC 2 cut(s) 788, 1008
AciI CCGC 4 cut(s) 275, 667, 788, 1006
AclWI GGATC 2 cut(s) 680, 820
AcyI GRCGYC 1 cut(s) 50
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 7 cut(s) 205, 631, 765, 932, 1052, 1107, 1159
AfiI CCNNNNNNNGG 5 cut(s) 164, 218, 625, 626, 676
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 3 cut(s) 332, 388, 908
AjnI CCWGG 2 cut(s) 535, 1108
AluBI AGCT 3 cut(s) 108, 705, 1076
AluI AGCT 3 cut(s) 108, 705, 1076
Alw21I GWGCWC 2 cut(s) 110, 990
Alw26I GTCTC 3 cut(s) 741, 778, 932
AlwI GGATC 2 cut(s) 680, 820
AlwNI CAGNNNCTG 1 cut(s) 46
Ama87I CYCGRG 2 cut(s) 164, 184
AoxI GGCC 5 cut(s) 65, 646, 679, 865, 1003
ApeKI GCWGC 3 cut(s) 371, 638, 954
ArsI GACNNNNNNTTYG 4 cut(s) 136, 168, 311, 343
Asp700I GAANNNNTTC 1 cut(s) 120
AspLEI GCGC 3 cut(s) 346, 657, 666
AspS9I GGNCC 6 cut(s) 65, 152, 647, 679, 812, 1003
AsuC2I CCSGG 3 cut(s) 74, 165, 166
AsuHPI GGTGA 5 cut(s) 293, 835, 889, 1046, 1161
AvaI CYCGRG 2 cut(s) 164, 184
AvaII GGWCC 2 cut(s) 152, 812
BaeGI GKGCMC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 2 cut(s) 110, 990
BbvI GCAGC 3 cut(s) 383, 650, 966
BccI CCATC 7 cut(s) 430, 587, 790, 974, 1039, 1060, 1174
BciT130I CCWGG 2 cut(s) 537, 1110
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 3 cut(s) 74, 165, 166
BcoDI GTCTC 3 cut(s) 741, 778, 932
BfaI CTAG 3 cut(s) 159, 683, 1062
BfmI CTRYAG 4 cut(s) 339, 462, 816, 967
BfoI RGCGCY 1 cut(s) 658
BisI GCNGC 6 cut(s) 372, 639, 667, 789, 955, 1006
BlsI GCNGC 6 cut(s) 373, 640, 668, 790, 956, 1007
BmcAI AGTACT 1 cut(s) 1159
Bme1390I CCNGG 5 cut(s) 74, 165, 166, 537, 1110
Bme18I GGWCC 2 cut(s) 152, 812
BmeT110I CYCGRG 2 cut(s) 164, 184
BmgT120I GGNCC 6 cut(s) 65, 152, 647, 679, 812, 1003
BmiI GGNNCC 2 cut(s) 649, 813
BmrFI CCNGG 5 cut(s) 74, 165, 166, 537, 1110
BmrI ACTGGG 1 cut(s) 943
BmsI GCATC 1 cut(s) 579
BmuI ACTGGG 1 cut(s) 943
BpiI GAAGAC 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 250
BpuMI CCSGG 3 cut(s) 74, 165, 166
BsaHI GRCGYC 1 cut(s) 50
BsaI GGTCTC 1 cut(s) 932
BsaJI CCNNGG 7 cut(s) 164, 183, 375, 620, 714, 1109, 1119
BsaWI WCCGGW 1 cut(s) 1026
Bsc4I CCNNNNNNNGG 5 cut(s) 164, 218, 625, 626, 676
Bse1I ACTGG 1 cut(s) 938
Bse3DI GCAATG 3 cut(s) 957, 990, 1131
BseBI CCWGG 2 cut(s) 537, 1110
BseDI CCNNGG 7 cut(s) 164, 183, 375, 620, 714, 1109, 1119
BseGI GGATG 3 cut(s) 321, 979, 1071
BseLI CCNNNNNNNGG 5 cut(s) 164, 218, 625, 626, 676
BseMI GCAATG 3 cut(s) 957, 990, 1131
BseNI ACTGG 1 cut(s) 938
BseRI GAGGAG 1 cut(s) 860
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 3 cut(s) 383, 650, 966
BseYI CCCAGC 2 cut(s) 625, 651
Bsh1285I CGRYCG 1 cut(s) 784
BshFI GGCC 5 cut(s) 67, 648, 681, 867, 1005
BsiEI CGRYCG 1 cut(s) 784
BsiHKAI GWGCWC 2 cut(s) 110, 990
BsiHKCI CYCGRG 2 cut(s) 164, 184
BsiSI CCGG 3 cut(s) 74, 165, 1027
BslFI GGGAC 2 cut(s) 147, 181
BslI CCNNNNNNNGG 5 cut(s) 164, 218, 625, 626, 676
BsmAI GTCTC 3 cut(s) 741, 778, 932
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 2 cut(s) 147, 181
BsnI GGCC 5 cut(s) 67, 648, 681, 867, 1005
Bso31I GGTCTC 1 cut(s) 932
BsoBI CYCGRG 2 cut(s) 164, 184
Bsp1286I GDGCHC 3 cut(s) 110, 142, 990
Bsp143I GATC 4 cut(s) 295, 685, 781, 825
Bsp19I CCATGG 1 cut(s) 1119
BspACI CCGC 4 cut(s) 275, 667, 788, 1006
BspANI GGCC 5 cut(s) 67, 648, 681, 867, 1005
BspLI GGNNCC 2 cut(s) 649, 813
BspMAI CTGCAG 2 cut(s) 466, 820
BspPI GGATC 2 cut(s) 680, 820
BspTNI GGTCTC 1 cut(s) 932
BsrBI CCGCTC 2 cut(s) 788, 1008
BsrDI GCAATG 3 cut(s) 957, 990, 1131
BsrI ACTGG 1 cut(s) 938
BssECI CCNNGG 7 cut(s) 164, 183, 375, 620, 714, 1109, 1119
BssMI GATC 4 cut(s) 295, 685, 781, 825
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 3 cut(s) 620, 714, 1119
Bst2UI CCWGG 2 cut(s) 537, 1110
Bst4CI ACNGT 4 cut(s) 604, 723, 928, 1021
BstACI GRCGYC 1 cut(s) 50
BstAPI GCANNNNNTGC 1 cut(s) 532
BstC8I GCNNGC 1 cut(s) 703
BstDSI CCRYGG 2 cut(s) 375, 1119
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 3 cut(s) 321, 979, 1071
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 3 cut(s) 346, 657, 666
BstKTI GATC 4 cut(s) 298, 688, 784, 828
BstMAI GTCTC 3 cut(s) 741, 778, 932
BstMBI GATC 4 cut(s) 295, 685, 781, 825
BstMCI CGRYCG 1 cut(s) 784
BstMWI GCNNNNNNNGC 8 cut(s) 11, 49, 532, 654, 661, 663, 702, 785
BstNI CCWGG 2 cut(s) 537, 1110
BstNSI RCATGY 2 cut(s) 415, 836
BstSCI CCNGG 5 cut(s) 72, 163, 164, 535, 1108
BstSFI CTRYAG 4 cut(s) 339, 462, 816, 967
BstSLI GKGCMC 1 cut(s) 142
BstV1I GCAGC 3 cut(s) 383, 650, 966
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 1 cut(s) 685
BstXI CCANNNNNNTGG 1 cut(s) 863
BstYI RGATCY 1 cut(s) 685
BsuRI GGCC 5 cut(s) 67, 648, 681, 867, 1005
BtgI CCRYGG 2 cut(s) 375, 1119
BtsCI GGATG 3 cut(s) 321, 979, 1071
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 3 cut(s) 44, 202, 728
Cac8I GCNNGC 1 cut(s) 703
CaiI CAGNNNCTG 1 cut(s) 46
CfoI GCGC 3 cut(s) 346, 657, 666
Cfr13I GGNCC 6 cut(s) 65, 152, 647, 679, 812, 1003
Cfr9I CCCGGG 1 cut(s) 164
CseI GACGC 1 cut(s) 58
Csp6I GTAC 7 cut(s) 204, 630, 764, 931, 1051, 1106, 1158
CviAII CATG 6 cut(s) 58, 412, 635, 833, 1120, 1173
CviQI GTAC 7 cut(s) 204, 630, 764, 931, 1051, 1106, 1158
DpnI GATC 4 cut(s) 297, 687, 783, 827
DpnII GATC 4 cut(s) 295, 685, 781, 825
DraIII CACNNNGTG 1 cut(s) 381
DrdI GACNNNNNNGTC 1 cut(s) 175
DseDI GACNNNNNNGTC 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 3 cut(s) 620, 714, 1119
Eco24I GRGCYC 1 cut(s) 110
Eco31I GGTCTC 1 cut(s) 932
Eco47I GGWCC 2 cut(s) 152, 812
Eco53kI GAGCTC 1 cut(s) 108
Eco88I CYCGRG 2 cut(s) 164, 184
EcoICRI GAGCTC 1 cut(s) 108
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO109I RGGNCCY 3 cut(s) 647, 679, 812
EcoRII CCWGG 2 cut(s) 535, 1108
EcoT14I CCWWGG 3 cut(s) 620, 714, 1119
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 3 cut(s) 620, 714, 1119
Esp3I CGTCTC 1 cut(s) 741
FaeI CATG 6 cut(s) 61, 415, 638, 836, 1123, 1176
FaqI GGGAC 2 cut(s) 147, 181
FatI CATG 6 cut(s) 57, 411, 634, 832, 1119, 1172
FauI CCCGC 1 cut(s) 282
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 6 cut(s) 372, 639, 667, 789, 955, 1006
FokI GGATG 3 cut(s) 308, 986, 1078
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 6 cut(s) 372, 639, 667, 789, 955, 1006
FspBI CTAG 3 cut(s) 159, 683, 1062
GlaI GCGC 3 cut(s) 345, 656, 665
GluI GCNGC 6 cut(s) 372, 639, 667, 789, 955, 1006
GsaI CCCAGC 2 cut(s) 629, 655
HaeII RGCGCY 1 cut(s) 658
HaeIII GGCC 5 cut(s) 67, 648, 681, 867, 1005
HapII CCGG 3 cut(s) 74, 165, 1027
HgaI GACGC 1 cut(s) 58
HhaI GCGC 3 cut(s) 346, 657, 666
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 6 cut(s) 61, 415, 638, 836, 1123, 1176
Hin6I GCGC 3 cut(s) 344, 655, 664
HinP1I GCGC 3 cut(s) 344, 655, 664
HindIII AAGCTT 2 cut(s) 703, 1074
HinfI GANTC 2 cut(s) 741, 887
HpaII CCGG 3 cut(s) 74, 165, 1027
HphI GGTGA 5 cut(s) 293, 835, 889, 1046, 1161
Hpy166II GTNNAC 3 cut(s) 38, 931, 1053
Hpy188I TCNGA 3 cut(s) 690, 746, 830
Hpy188III TCNNGA 2 cut(s) 125, 186
Hpy8I GTNNAC 3 cut(s) 38, 931, 1053
HpyAV CCTTC 3 cut(s) 56, 83, 317
HpyCH4III ACNGT 4 cut(s) 604, 723, 928, 1021
HpyCH4V TGCA 5 cut(s) 82, 419, 464, 638, 818
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 49, 532, 654, 661, 663, 702, 785
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 6 cut(s) 61, 415, 638, 836, 1123, 1176
HspAI GCGC 3 cut(s) 344, 655, 664
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 4 cut(s) 295, 685, 781, 825
LmnI GCTCC 4 cut(s) 271, 426, 1080, 1141
Lsp1109I GCAGC 3 cut(s) 383, 650, 966
LweI GCATC 1 cut(s) 579
MaeI CTAG 3 cut(s) 159, 683, 1062
MaeIII GTNAC 1 cut(s) 401
MalI GATC 4 cut(s) 297, 687, 783, 827
MbiI CCGCTC 2 cut(s) 788, 1008
MboI GATC 4 cut(s) 295, 685, 781, 825
MboII GAAGA 5 cut(s) 31, 445, 480, 490, 1082
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 3 cut(s) 110, 142, 990
MluCI AATT 2 cut(s) 172, 291
MlyI GAGTC 1 cut(s) 881
MmeI TCCRAC 2 cut(s) 544, 853
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 2 cut(s) 315, 1101
MslI CAYNNNNRTG 2 cut(s) 575, 1171
MspI CCGG 3 cut(s) 74, 165, 1027
MspR9I CCNGG 5 cut(s) 74, 165, 166, 537, 1110
MunI CAATTG 1 cut(s) 291
MvaI CCWGG 2 cut(s) 537, 1110
MwoI GCNNNNNNNGC 8 cut(s) 11, 49, 532, 654, 661, 663, 702, 785
NciI CCSGG 3 cut(s) 74, 165, 166
NcoI CCATGG 1 cut(s) 1119
NdeII GATC 4 cut(s) 295, 685, 781, 825
NlaIII CATG 6 cut(s) 61, 415, 638, 836, 1123, 1176
NlaIV GGNNCC 2 cut(s) 649, 813
NspI RCATGY 2 cut(s) 415, 836
PciI ACATGT 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 741
PkrI GCNGC 6 cut(s) 373, 640, 668, 790, 956, 1007
Ple19I CGATCG 1 cut(s) 784
PleI GAGTC 1 cut(s) 881
PpsI GAGTC 1 cut(s) 881
PpuMI RGGWCCY 1 cut(s) 812
PscI ACATGT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 812
Psp6I CCWGG 2 cut(s) 535, 1108
PspFI CCCAGC 2 cut(s) 625, 651
PspGI CCWGG 2 cut(s) 535, 1108
PspN4I GGNNCC 2 cut(s) 649, 813
PspPI GGNCC 6 cut(s) 65, 152, 647, 679, 812, 1003
PspPPI RGGWCCY 1 cut(s) 812
PstI CTGCAG 2 cut(s) 466, 820
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 685
PvuI CGATCG 1 cut(s) 784
RsaI GTAC 7 cut(s) 205, 631, 765, 932, 1052, 1107, 1159
RsaNI GTAC 7 cut(s) 204, 630, 764, 931, 1051, 1106, 1158
RseI CAYNNNNRTG 2 cut(s) 575, 1171
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 2 cut(s) 315, 1101
SatI GCNGC 6 cut(s) 372, 639, 667, 789, 955, 1006
Sau3AI GATC 4 cut(s) 295, 685, 781, 825
Sau96I GGNCC 6 cut(s) 65, 152, 647, 679, 812, 1003
ScaI AGTACT 1 cut(s) 1159
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 5 cut(s) 74, 165, 166, 537, 1110
SduI GDGCHC 3 cut(s) 110, 142, 990
SfaNI GCATC 1 cut(s) 579
SfcI CTRYAG 4 cut(s) 339, 462, 816, 967
SinI GGWCC 2 cut(s) 152, 812
SmaI CCCGGG 1 cut(s) 166
SmiMI CAYNNNNRTG 2 cut(s) 575, 1171
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
Sse9I AATT 2 cut(s) 172, 291
SsiI CCGC 4 cut(s) 275, 667, 788, 1006
SspMI CTAG 3 cut(s) 159, 683, 1062
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 5 cut(s) 72, 163, 164, 535, 1108
StyI CCWWGG 3 cut(s) 620, 714, 1119
TaaI ACNGT 4 cut(s) 604, 723, 928, 1021
TaqI TCGA 3 cut(s) 784, 978, 1043
TasI AATT 2 cut(s) 172, 291
TatI WGTACW 4 cut(s) 763, 930, 1050, 1157
TauI GCSGC 3 cut(s) 669, 791, 1008
TfiI GAWTC 1 cut(s) 741
Tru1I TTAA 2 cut(s) 315, 1101
Tru9I TTAA 2 cut(s) 315, 1101
TscAI CASTG 3 cut(s) 51, 202, 728
TseI GCWGC 3 cut(s) 371, 638, 954
TspDTI ATGAA 1 cut(s) 1120
TspGWI ACGGA 1 cut(s) 467
TspMI CCCGGG 1 cut(s) 164
TspRI CASTG 3 cut(s) 51, 202, 728
VpaK11BI GGWCC 2 cut(s) 152, 812
XagI CCTNNNNNAGG 1 cut(s) 674
XceI RCATGY 2 cut(s) 415, 836
XmaI CCCGGG 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 3 cut(s) 159, 683, 1062
ZrmI AGTACT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.