FvH4_3g14680

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0017624
Physical Location & Seq
Reverse (-)
9051293 .. 9052915
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14680.t1

Sequence Viewer

Length: 1623 bp
ATGGAGGACTTCAATGCTTGGCTCTCCTTCCCAATCCATGAGAATCTCAACCATGATCTCGCTATTCGAAGGTTCTGTCCAGCAAGACTTGAGCAAGAACAAGGTGGAGGAGAGTGGGAGGAGATACTAAATGAGATTGAGTTTTGTTCACCCTCTTATGATTCAACCACCACTCCTTGCTTGCCTGCTTCTGAAGTTGATGAGTTTGTTGACAGCATCATCAACATGGATCACTGCAAGGATGTCAACGACGATTATCATCAATCCCTGGAGAAACAACAAAGTAGTTTTGATTACTTCCAAGATGGCATGCAGACATTTTCAATGGAGGATGATTTGTATGGAGATGTCATGATGATGATGGAAGGAGATGATCAGATGGAGATGTGTGGCACAGTTGAGGATTTTGGAGTTGTAGATGATCAAACTAGTATGGTGCCTAGTATTGAAGAGGTAAGCCATGGTGTGGACCAAGGGCTTCACTTGGTGCACATGTTACTGGCTTGTGCTGAAGCTGTGGGGTGCAGGGACACAAGGCTTGCAGAATCATTGCTCACCCAAATCTGGGCTTCGGTTAGTCCTTTCGGCGATTCCTTGCAGAGAGTATCATACTGCTTCGCAATGGGGTTGAAGTCAAGGCTCTCTCTTCTTCAAAATGTGAATTTAAATGGTACATTCAACAATGACACTATGGATGTGTCGATGATCGCTAGGGAGGAGAAACTGGAGGCTTTTCAACTCCTTTACCAGACAACTCCCTACATAGCTTTCGGATTCATGGTTGCAAATGAAGCTATATGTCAAGCAGCGCAAGGGAAGGAGAGCTTGCATATCATTGATTTGGGAATGGAGCACACTCTTCAATGGCCATCCTTGTTAAGAACCCTTGCATCAAGACCCGAAGGCCCGCCTAAGAAACTCCGAATCACAGGACTTGTTGAAGATGATAATGTCTTCGAGCTTGAAGCTGGGATGAGAGTTCTGGTGGAGGAAGCTAGCTCACTTGGGATACCAATGGAATTCAACATGATCTCGGAGCCGGTAAAACCATCACTTCTAACTAAAGAGAATCTAGACTTGAGAGAAGGGGAAGCATTATGTGTCAATAGCATAATGCACCTACATAGGTATGTCAGAGAGAGTAGAGGGTCTTTGAAGGCAATCCTCCAAGCATTGAAGAAACTAAGTCCTAGTGTGCTTACATTAGTAGAACAAGATGCAAACCACAACGGGCCATTTTTTCTTGGAAGATTCCTCGAGTCTCTTCACTTTTATTCTGCAATTTTCGACTCACTTGAGGTGAGTCTGCCTCGAAACAGCCCTCAGAGGACGAAGATAGAGAGACTTCACTTTGCAGATGAAATTAGGAACATTGTTGCCTTTGAGGGATTAAATAGGGTTGAGAGGCATGAAAGGGCAGACCAATGGAGAAGGCAACTAGGCAGAGCTGGGTTTCAAGTTATGGGATTGAAATGTACAAGTCAAGCTAGGATGATGCTATCTGTTTATGGCTGTGATGGGTACAGTTTGGCTAGTGAGAAGGGTTGTCTCCTCCTTGGGTGGAAGGGAAGACCCATAATGTTGGCAGCTGCATGGCAAGTGAACAATGCCTCTTCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

541

Amino Acids

60.88

Weight (kDa)

4.76

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 162 - 533 4.1e-108 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g14680
malus_domestica MD05G1229500.v1.1
prunus_persica Prupe.4G132100_v2.0.a1
pyrus_communis pycom05g21040
rosa_chinensis RchiOBHm_Chr5g0024171
rosa_laevigata RLG00000032776
rosa_multiflora Rmu_sc0004033.1_g000010
rosa_rugosa Rorug05G0082200
rosa_samantha Rh5BG169400 Rh5CG185300 Rh5DG171700
rosa_wichuraiana Rw5G015590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 436
AccB7I CCANNNNNTGG 1 cut(s) 466
AciI CCGC 1 cut(s) 908
AclWI GGATC 1 cut(s) 237
AcoI YGGCCR 1 cut(s) 866
AcsI RAATTY 2 cut(s) 661, 1019
AcuI CTGAAG 2 cut(s) 213, 531
AdeI CACNNNGTG 1 cut(s) 487
AfaI GTAC 3 cut(s) 673, 1477, 1523
AfiI CCNNNNNNNGG 3 cut(s) 466, 564, 565
AflIII ACRYGT 1 cut(s) 492
AhlI ACTAGT 1 cut(s) 428
AjnI CCWGG 1 cut(s) 267
Alw21I GWGCWC 2 cut(s) 492, 855
Alw26I GTCTC 3 cut(s) 1266, 1336, 1553
Alw44I GTGCAC 1 cut(s) 488
AlwI GGATC 1 cut(s) 237
Ama87I CYCGRG 1 cut(s) 1256
AoxI GGCC 3 cut(s) 866, 904, 1232
ApaLI GTGCAC 1 cut(s) 488
ApeKI GCWGC 3 cut(s) 806, 1586, 1589
ApoI RAATTY 2 cut(s) 661, 1019
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspLEI GCGC 1 cut(s) 811
AspS9I GGNCC 3 cut(s) 469, 905, 1232
AsuHPI GGTGA 3 cut(s) 141, 547, 1312
AsuII TTCGAA 1 cut(s) 67
AsuNHI GCTAGC 1 cut(s) 995
AvaI CYCGRG 1 cut(s) 1256
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 492
BalI TGGCCA 1 cut(s) 868
BanI GGYRCC 1 cut(s) 436
BbsI GAAGAC 2 cut(s) 946, 1576
Bbv12I GWGCWC 2 cut(s) 492, 855
BbvI GCAGC 3 cut(s) 818, 1576, 1598
BccI CCATC 6 cut(s) 299, 355, 373, 877, 1057, 1511
BciT130I CCWGG 1 cut(s) 269
BciVI GTATCC 1 cut(s) 1002
BclI TGATCA 2 cut(s) 373, 421
BcoDI GTCTC 3 cut(s) 1266, 1336, 1553
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 9 cut(s) 429, 441, 711, 996, 1073, 1191, 1439, 1488, 1533
BfuI GTATCC 1 cut(s) 1002
BisI GCNGC 3 cut(s) 807, 1587, 1590
BlsI GCNGC 3 cut(s) 808, 1588, 1591
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 469
BmeT110I CYCGRG 1 cut(s) 1256
BmgT120I GGNCC 3 cut(s) 469, 905, 1232
BmiI GGNNCC 2 cut(s) 438, 1038
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 4 cut(s) 225, 899, 1207, 1485
BmtI GCTAGC 1 cut(s) 999
BpiI GAAGAC 2 cut(s) 946, 1576
BplI GAGNNNNNCTC 2 cut(s) 1294, 1326
BpmI CTGGAG 2 cut(s) 290, 746
Bpu14I TTCGAA 1 cut(s) 67
BpuEI CTTGAG 3 cut(s) 110, 1099, 1316
BsaBI GATNNNNATC 1 cut(s) 258
BsaJI CCNNGG 4 cut(s) 267, 460, 472, 1555
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 3 cut(s) 466, 564, 565
Bse118I RCCGGY 1 cut(s) 1039
Bse1I ACTGG 2 cut(s) 504, 729
Bse3DI GCAATG 2 cut(s) 548, 627
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 4 cut(s) 267, 460, 472, 1555
BseGI GGATG 6 cut(s) 247, 337, 700, 869, 978, 1497
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 3 cut(s) 466, 564, 565
BseMI GCAATG 2 cut(s) 548, 627
BseMII CTCAG 1 cut(s) 1337
BseNI ACTGG 2 cut(s) 504, 729
BseRI GAGGAG 4 cut(s) 123, 134, 731, 1541
BseSI GKGCMC 1 cut(s) 492
BseXI GCAGC 3 cut(s) 818, 1576, 1598
BseYI CCCAGC 2 cut(s) 968, 1448
BsgI GTGCAG 1 cut(s) 544
BshFI GGCC 3 cut(s) 868, 906, 1234
BshNI GGYRCC 1 cut(s) 436
BsiHKAI GWGCWC 2 cut(s) 492, 855
BsiHKCI CYCGRG 1 cut(s) 1256
BsiSI CCGG 1 cut(s) 1040
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 3 cut(s) 466, 564, 565
BsmAI GTCTC 3 cut(s) 1266, 1336, 1553
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 3 cut(s) 868, 906, 1234
BsoBI CYCGRG 1 cut(s) 1256
Bsp119I TTCGAA 1 cut(s) 67
Bsp1286I GDGCHC 2 cut(s) 492, 855
Bsp1407I TGTACA 1 cut(s) 1475
Bsp143I GATC 6 cut(s) 55, 229, 373, 421, 705, 1029
Bsp19I CCATGG 1 cut(s) 460
BspACI CCGC 1 cut(s) 908
BspANI GGCC 3 cut(s) 868, 906, 1234
BspCNI CTCAG 1 cut(s) 1336
BspHI TCATGA 1 cut(s) 351
BspLI GGNNCC 2 cut(s) 438, 1038
BspOI GCTAGC 1 cut(s) 999
BspPI GGATC 1 cut(s) 237
BspT104I TTCGAA 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 436
BsrDI GCAATG 2 cut(s) 548, 627
BsrFI RCCGGY 1 cut(s) 1039
BsrGI TGTACA 1 cut(s) 1475
BsrI ACTGG 2 cut(s) 504, 729
BssAI RCCGGY 1 cut(s) 1039
BssECI CCNNGG 4 cut(s) 267, 460, 472, 1555
BssMI GATC 6 cut(s) 55, 229, 373, 421, 705, 1029
BssT1I CCWWGG 3 cut(s) 460, 472, 1555
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 397, 1526
Bst6I CTCTTC 5 cut(s) 444, 651, 864, 1269, 1618
BstAUI TGTACA 1 cut(s) 1475
BstBI TTCGAA 1 cut(s) 67
BstC8I GCNNGC 7 cut(s) 182, 186, 311, 540, 827, 908, 997
BstDEI CTNAG 4 cut(s) 912, 1184, 1323, 1620
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 6 cut(s) 247, 337, 700, 869, 978, 1497
BstHHI GCGC 1 cut(s) 811
BstKTI GATC 6 cut(s) 58, 232, 376, 424, 708, 1032
BstMAI GTCTC 3 cut(s) 1266, 1336, 1553
BstMBI GATC 6 cut(s) 55, 229, 373, 421, 705, 1029
BstMWI GCNNNNNNNGC 1 cut(s) 791
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 2 cut(s) 313, 496
BstSCI CCNGG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 818, 1576, 1598
BstV2I GAAGAC 2 cut(s) 946, 1576
BstXI CCANNNNNNTGG 1 cut(s) 1582
BsuI GTATCC 1 cut(s) 1002
BsuRI GGCC 3 cut(s) 868, 906, 1234
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 6 cut(s) 247, 337, 700, 869, 978, 1497
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 7 cut(s) 182, 186, 311, 540, 827, 908, 997
CciI TCATGA 1 cut(s) 351
CfoI GCGC 1 cut(s) 811
Cfr10I RCCGGY 1 cut(s) 1039
Cfr13I GGNCC 3 cut(s) 469, 905, 1232
Csp6I GTAC 3 cut(s) 672, 1476, 1522
CviQI GTAC 3 cut(s) 672, 1476, 1522
DdeI CTNAG 4 cut(s) 912, 1184, 1323, 1620
DpnI GATC 6 cut(s) 57, 231, 375, 423, 707, 1031
DpnII GATC 6 cut(s) 55, 229, 373, 421, 705, 1029
DraI TTTAAA 1 cut(s) 666
DraIII CACNNNGTG 1 cut(s) 487
EaeI YGGCCR 1 cut(s) 866
Eam1104I CTCTTC 5 cut(s) 444, 651, 864, 1269, 1618
EarI CTCTTC 5 cut(s) 444, 651, 864, 1269, 1618
Eco130I CCWWGG 3 cut(s) 460, 472, 1555
Eco47I GGWCC 1 cut(s) 469
Eco57I CTGAAG 2 cut(s) 213, 531
Eco88I CYCGRG 1 cut(s) 1256
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 3 cut(s) 460, 472, 1555
ErhI CCWWGG 3 cut(s) 460, 472, 1555
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 1 cut(s) 542
FauI CCCGC 1 cut(s) 915
FbaI TGATCA 2 cut(s) 373, 421
Fnu4HI GCNGC 3 cut(s) 807, 1587, 1590
FokI GGATG 6 cut(s) 254, 344, 707, 856, 985, 1504
Fsp4HI GCNGC 3 cut(s) 807, 1587, 1590
FspBI CTAG 9 cut(s) 429, 441, 711, 996, 1073, 1191, 1439, 1488, 1533
GlaI GCGC 1 cut(s) 810
GluI GCNGC 3 cut(s) 807, 1587, 1590
GsaI CCCAGC 2 cut(s) 972, 1452
GsuI CTGGAG 2 cut(s) 290, 746
HaeIII GGCC 3 cut(s) 868, 906, 1234
HapII CCGG 1 cut(s) 1040
HhaI GCGC 1 cut(s) 811
Hin6I GCGC 1 cut(s) 809
HinP1I GCGC 1 cut(s) 809
HincII GTYRAC 2 cut(s) 211, 247
HindII GTYRAC 2 cut(s) 211, 247
HpaII CCGG 1 cut(s) 1040
HphI GGTGA 3 cut(s) 141, 547, 1312
Hpy166II GTNNAC 6 cut(s) 149, 211, 247, 469, 490, 1603
Hpy188I TCNGA 7 cut(s) 193, 378, 773, 923, 1036, 1136, 1326
Hpy188III TCNNGA 3 cut(s) 352, 894, 1073
Hpy8I GTNNAC 6 cut(s) 149, 211, 247, 469, 490, 1603
Hpy99I CGWCG 1 cut(s) 254
HpyCH4III ACNGT 2 cut(s) 397, 1526
HpyF10VI GCNNNNNNNGC 1 cut(s) 791
HpyF3I CTNAG 4 cut(s) 912, 1184, 1323, 1620
HspAI GCGC 1 cut(s) 809
Ksp22I TGATCA 2 cut(s) 373, 421
Kzo9I GATC 6 cut(s) 55, 229, 373, 421, 705, 1029
LmnI GCTCC 2 cut(s) 850, 1036
Lsp1109I GCAGC 3 cut(s) 818, 1576, 1598
LweI GCATC 4 cut(s) 225, 899, 1207, 1485
MaeI CTAG 9 cut(s) 429, 441, 711, 996, 1073, 1191, 1439, 1488, 1533
MaeIII GTNAC 1 cut(s) 495
MalI GATC 6 cut(s) 57, 231, 375, 423, 707, 1031
MboI GATC 6 cut(s) 55, 229, 373, 421, 705, 1029
MhlI GDGCHC 2 cut(s) 492, 855
MlsI TGGCCA 1 cut(s) 868
MluCI AATT 4 cut(s) 661, 1019, 1281, 1362
MluNI TGGCCA 1 cut(s) 868
MlyI GAGTC 3 cut(s) 1268, 1283, 1312
Mox20I TGGCCA 1 cut(s) 868
MscI TGGCCA 1 cut(s) 868
MseI TTAA 3 cut(s) 665, 878, 1391
MslI CAYNNNNRTG 3 cut(s) 224, 356, 1128
Msp20I TGGCCA 1 cut(s) 868
MspA1I CMGCKG 1 cut(s) 1589
MspI CCGG 1 cut(s) 1040
MspR9I CCNGG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 791
NcoI CCATGG 1 cut(s) 460
NdeII GATC 6 cut(s) 55, 229, 373, 421, 705, 1029
NheI GCTAGC 1 cut(s) 995
NlaIV GGNNCC 2 cut(s) 438, 1038
NspI RCATGY 2 cut(s) 313, 496
NspV TTCGAA 1 cut(s) 67
PaeI GCATGC 1 cut(s) 313
PaeR7I CTCGAG 1 cut(s) 1256
PagI TCATGA 1 cut(s) 351
PciI ACATGT 1 cut(s) 492
PfeI GAWTC 8 cut(s) 43, 161, 545, 590, 774, 924, 1069, 1251
PflMI CCANNNNNTGG 1 cut(s) 466
PkrI GCNGC 3 cut(s) 808, 1588, 1591
PleI GAGTC 3 cut(s) 1267, 1283, 1311
PpsI GAGTC 3 cut(s) 1267, 1283, 1311
PscI ACATGT 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 267
PspFI CCCAGC 2 cut(s) 968, 1448
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 438, 1038
PspPI GGNCC 3 cut(s) 469, 905, 1232
PspXI VCTCGAGB 1 cut(s) 1256
PvuII CAGCTG 1 cut(s) 1589
RsaI GTAC 3 cut(s) 673, 1477, 1523
RsaNI GTAC 3 cut(s) 672, 1476, 1522
RseI CAYNNNNRTG 3 cut(s) 224, 356, 1128
SaqAI TTAA 3 cut(s) 665, 878, 1391
SatI GCNGC 3 cut(s) 807, 1587, 1590
Sau3AI GATC 6 cut(s) 55, 229, 373, 421, 705, 1029
Sau96I GGNCC 3 cut(s) 469, 905, 1232
SchI GAGTC 3 cut(s) 1268, 1283, 1312
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 2 cut(s) 492, 855
SfaNI GCATC 4 cut(s) 225, 899, 1207, 1485
Sfr274I CTCGAG 1 cut(s) 1256
SfuI TTCGAA 1 cut(s) 67
SinI GGWCC 1 cut(s) 469
SlaI CTCGAG 1 cut(s) 1256
SmiI ATTTAAAT 1 cut(s) 666
SmiMI CAYNNNNRTG 3 cut(s) 224, 356, 1128
SmlI CTYRAG 4 cut(s) 89, 1078, 1256, 1295
SmoI CTYRAG 4 cut(s) 89, 1078, 1256, 1295
SpeI ACTAGT 1 cut(s) 428
SphI GCATGC 1 cut(s) 313
Sse9I AATT 4 cut(s) 661, 1019, 1281, 1362
SsiI CCGC 1 cut(s) 908
SspMI CTAG 9 cut(s) 429, 441, 711, 996, 1073, 1191, 1439, 1488, 1533
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 3 cut(s) 460, 472, 1555
SwaI ATTTAAAT 1 cut(s) 666
TaaI ACNGT 2 cut(s) 397, 1526
TaqI TCGA 6 cut(s) 67, 701, 957, 1257, 1287, 1312
TasI AATT 4 cut(s) 661, 1019, 1281, 1362
TatI WGTACW 1 cut(s) 1475
TfiI GAWTC 8 cut(s) 43, 161, 545, 590, 774, 924, 1069, 1251
Tru1I TTAA 3 cut(s) 665, 878, 1391
Tru9I TTAA 3 cut(s) 665, 878, 1391
TscAI CASTG 1 cut(s) 239
TseI GCWGC 3 cut(s) 806, 1586, 1589
TspDTI ATGAA 4 cut(s) 766, 804, 1374, 1425
TspRI CASTG 1 cut(s) 239
Van91I CCANNNNNTGG 1 cut(s) 466
VneI GTGCAC 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 2 cut(s) 661, 1019
XbaI TCTAGA 1 cut(s) 1072
XceI RCATGY 2 cut(s) 313, 496
XhoI CTCGAG 1 cut(s) 1256
XspI CTAG 9 cut(s) 429, 441, 711, 996, 1073, 1191, 1439, 1488, 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.