pycom05g21040

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0017624
Physical Location & Seq
Reverse (-)
23738488 .. 23739618
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21040.1

Sequence Viewer

Length: 1131 bp
ATGTTGCTCGCTTGCGCCGAGGCTGTTGGCTGCAGGGACACCAAGCTTGCTGACTCATTGCTCGCCCAAATTTGGGCATCTGTGAGCCCTTGGGGCGACTCCTTGCAAAGAGTTTCATACTGCTTTGCAATGGGATTGAAATCAAGGCTTTCGCTTCTTCAAAATGTCAACATAAATGGCACATTCAACAACGATATGATGGATGTTTCAGTGACCTCTAGGGACGACAAGTTGGAAGCTTTTCAACTCCTTAACCAAACAACTCCTTACATCGCTTTCGGTTTCATGGTTGCCAATGAAGCTATATGCCAAGCAGCGCAAGGGAAGGAAGACTTGCACATCATTGATTTGGGAATGGAGCACACTCTTCAGTGGCCTTCTTTGATCAGAACCCTAGCATCGAGACCCGAAGGCCCGCCTAAGAAGCTGCGAATCACGGGACTTGTTGAAGATCACAATCTGTTTGAGCTTGAAGCTGGCATGAGAGCTCTGGCGGAGGAGGCTAGCTCGCTAGGGATACCAACAGAATTTAATTTGATATCGGAGCCGGTGAAACCATCACTCCTAACTAGAGAGAACCTAGACTTGAGAGAAGGGGAAGCATTATTTGTCAATAGCATCATGCACTTGCATAAGTATGTCAAAGAGAGTAGAGGCTCTCTGAAGGCAATTCTTCAAGCTCTGAAGAAATTAGGTCCATCCGTGCTTACATTGGTAGAACAAGATGCAAATCACAACGGGCCATTTTTCCTCGGGAGATTCCTCGAGTCTCTTCACTTTTACTCAGCAATTTTCGACTCACTCGAGGCTAGCCTCCCGCGAAACAGCCCTCAGAGGATGAAGATCGAGAGGCAGCACTTCGCGGACGAAATCAGAAACATTGTTGCTTTTGAGGGATTAAATAGGATTGAGAGGCATGAGAGGGCAGACCAGTGGAGAAGGCAATTAGGGCGAGCCGGGTTTCAAGTGATGGGGTTGAAGTGTACAAGTCAAGCAAGGATGATGCTATCTGTTTATGGCTGTGATGGCTACACTTTGGCTAGTGAGAAGGGTTGTCTCCTCCTCGGGTGGAAAGGCAGGCCGATAATGCTGGCATCGGCATGGCAAGCGCACGGCGTGTCTTCCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

377

Amino Acids

41.96

Weight (kDa)

6.57

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 2 - 369 5.1e-108 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g14680
malus_domestica MD05G1229500.v1.1
prunus_persica Prupe.4G132100_v2.0.a1
pyrus_communis pycom05g21040
rosa_chinensis RchiOBHm_Chr5g0024171
rosa_laevigata RLG00000032776
rosa_multiflora Rmu_sc0004033.1_g000010
rosa_rugosa Rorug05G0082200
rosa_samantha Rh5BG169400 Rh5CG185300 Rh5DG171700
rosa_wichuraiana Rw5G015590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 820, 863
AciI CCGC 4 cut(s) 416, 494, 818, 863
AcsI RAATTY 2 cut(s) 69, 527
AcuI CTGAAG 3 cut(s) 353, 683, 704
AdeI CACNNNGTG 1 cut(s) 1117
AfaI GTAC 1 cut(s) 985
AfiI CCNNNNNNNGG 2 cut(s) 72, 73
AgsI TTSAA 9 cut(s) 139, 161, 187, 245, 449, 473, 677, 965, 979
AluBI AGCT 9 cut(s) 46, 239, 302, 427, 469, 476, 488, 507, 680
AluI AGCT 9 cut(s) 46, 239, 302, 427, 469, 476, 488, 507, 680
Alw21I GWGCWC 2 cut(s) 363, 490
Alw26I GTCTC 3 cut(s) 397, 774, 1061
Ama87I CYCGRG 4 cut(s) 752, 764, 803, 1064
AoxI GGCC 4 cut(s) 374, 412, 740, 1079
ApeKI GCWGC 4 cut(s) 30, 314, 427, 853
ApoI RAATTY 2 cut(s) 69, 527
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 3 cut(s) 17, 319, 1111
AspS9I GGNCC 3 cut(s) 413, 695, 740
AsuC2I CCSGG 1 cut(s) 958
AsuHPI GGTGA 1 cut(s) 562
AsuNHI GCTAGC 2 cut(s) 503, 809
AvaI CYCGRG 4 cut(s) 752, 764, 803, 1064
AvaII GGWCC 1 cut(s) 695
BanII GRGCYC 2 cut(s) 89, 490
BbsI GAAGAC 2 cut(s) 336, 1113
Bbv12I GWGCWC 2 cut(s) 363, 490
BbvI GCAGC 4 cut(s) 17, 326, 414, 865
BccI CCATC 5 cut(s) 193, 565, 706, 964, 1019
BceAI ACGGC 1 cut(s) 1129
BciVI GTATCC 1 cut(s) 510
BclI TGATCA 1 cut(s) 384
BcnI CCSGG 1 cut(s) 958
BcoDI GTCTC 3 cut(s) 397, 774, 1061
BfaI CTAG 9 cut(s) 219, 395, 504, 512, 570, 581, 810, 1041, 1129
BfmI CTRYAG 1 cut(s) 31
BfuI GTATCC 1 cut(s) 510
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 4 cut(s) 31, 315, 428, 854
BlsI GCNGC 4 cut(s) 32, 316, 429, 855
Bme1390I CCNGG 1 cut(s) 958
Bme18I GGWCC 1 cut(s) 695
BmeT110I CYCGRG 4 cut(s) 752, 764, 803, 1064
BmgT120I GGNCC 3 cut(s) 413, 695, 740
BmiI GGNNCC 1 cut(s) 546
BmrFI CCNGG 1 cut(s) 958
BmsI GCATC 6 cut(s) 86, 407, 627, 715, 993, 1103
BmtI GCTAGC 2 cut(s) 507, 813
BpiI GAAGAC 2 cut(s) 336, 1113
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BpuEI CTTGAG 1 cut(s) 607
BpuMI CCSGG 1 cut(s) 958
BsaBI GATNNNNATC 4 cut(s) 139, 456, 729, 842
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 4 cut(s) 18, 89, 751, 1063
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 73
Bse118I RCCGGY 1 cut(s) 547
Bse1I ACTGG 1 cut(s) 931
Bse3DI GCAATG 2 cut(s) 56, 135
Bse8I GATNNNNATC 4 cut(s) 139, 456, 729, 842
BseDI CCNNGG 4 cut(s) 18, 89, 751, 1063
BseGI GGATG 4 cut(s) 208, 698, 843, 1005
BseJI GATNNNNATC 4 cut(s) 139, 456, 729, 842
BseLI CCNNNNNNNGG 2 cut(s) 72, 73
BseMI GCAATG 2 cut(s) 56, 135
BseMII CTCAG 2 cut(s) 798, 845
BseNI ACTGG 1 cut(s) 931
BseRI GAGGAG 3 cut(s) 512, 1049, 1052
BseXI GCAGC 4 cut(s) 17, 326, 414, 865
Bsh1236I CGCG 2 cut(s) 820, 863
BshFI GGCC 4 cut(s) 376, 414, 742, 1081
BsiHKAI GWGCWC 2 cut(s) 363, 490
BsiHKCI CYCGRG 4 cut(s) 752, 764, 803, 1064
BsiSI CCGG 2 cut(s) 548, 957
BslFI GGGAC 3 cut(s) 50, 236, 453
BslI CCNNNNNNNGG 2 cut(s) 72, 73
BsmAI GTCTC 3 cut(s) 397, 774, 1061
BsmFI GGGAC 3 cut(s) 50, 236, 453
BsnI GGCC 4 cut(s) 376, 414, 742, 1081
Bso31I GGTCTC 1 cut(s) 397
BsoBI CYCGRG 4 cut(s) 752, 764, 803, 1064
Bsp1286I GDGCHC 3 cut(s) 89, 363, 490
Bsp1407I TGTACA 1 cut(s) 983
Bsp143I GATC 3 cut(s) 384, 451, 843
BspACI CCGC 4 cut(s) 416, 494, 818, 863
BspANI GGCC 4 cut(s) 376, 414, 742, 1081
BspCNI CTCAG 2 cut(s) 797, 844
BspFNI CGCG 2 cut(s) 820, 863
BspLI GGNNCC 1 cut(s) 546
BspMAI CTGCAG 1 cut(s) 35
BspOI GCTAGC 2 cut(s) 507, 813
BspTNI GGTCTC 1 cut(s) 397
BsrDI GCAATG 2 cut(s) 56, 135
BsrFI RCCGGY 1 cut(s) 547
BsrGI TGTACA 1 cut(s) 983
BsrI ACTGG 1 cut(s) 931
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 4 cut(s) 18, 89, 751, 1063
BssMI GATC 3 cut(s) 384, 451, 843
BssT1I CCWWGG 1 cut(s) 89
Bst6I CTCTTC 2 cut(s) 372, 777
BstAUI TGTACA 1 cut(s) 983
BstDEI CTNAG 3 cut(s) 420, 784, 831
BstF5I GGATG 4 cut(s) 208, 698, 843, 1005
BstFNI CGCG 2 cut(s) 820, 863
BstHHI GCGC 3 cut(s) 17, 319, 1111
BstKTI GATC 3 cut(s) 387, 454, 846
BstMAI GTCTC 3 cut(s) 397, 774, 1061
BstMBI GATC 3 cut(s) 384, 451, 843
BstMWI GCNNNNNNNGC 8 cut(s) 93, 299, 424, 500, 949, 1026, 1087, 1106
BstSCI CCNGG 1 cut(s) 956
BstSFI CTRYAG 1 cut(s) 31
BstUI CGCG 2 cut(s) 820, 863
BstV1I GCAGC 4 cut(s) 17, 326, 414, 865
BstV2I GAAGAC 2 cut(s) 336, 1113
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 4 cut(s) 376, 414, 742, 1081
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 4 cut(s) 208, 698, 843, 1005
BtsIMutI CAGTG 3 cut(s) 216, 377, 938
CfoI GCGC 3 cut(s) 17, 319, 1111
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 3 cut(s) 413, 695, 740
Csp6I GTAC 1 cut(s) 984
CviAII CATG 5 cut(s) 286, 481, 622, 917, 1101
CviQI GTAC 1 cut(s) 984
DdeI CTNAG 3 cut(s) 420, 784, 831
DpnI GATC 3 cut(s) 386, 453, 845
DpnII GATC 3 cut(s) 384, 451, 843
DraIII CACNNNGTG 1 cut(s) 1117
Eam1104I CTCTTC 2 cut(s) 372, 777
EarI CTCTTC 2 cut(s) 372, 777
EciI GGCGGA 1 cut(s) 509
Ecl136II GAGCTC 1 cut(s) 488
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 2 cut(s) 89, 490
Eco31I GGTCTC 1 cut(s) 397
Eco32I GATATC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 695
Eco53kI GAGCTC 1 cut(s) 488
Eco57I CTGAAG 3 cut(s) 353, 683, 704
Eco88I CYCGRG 4 cut(s) 752, 764, 803, 1064
EcoICRI GAGCTC 1 cut(s) 488
EcoRV GATATC 1 cut(s) 540
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 2 cut(s) 89, 490
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 5 cut(s) 289, 484, 625, 920, 1104
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 3 cut(s) 50, 236, 453
FatI CATG 5 cut(s) 285, 480, 621, 916, 1100
FauI CCCGC 2 cut(s) 423, 825
FbaI TGATCA 1 cut(s) 384
Fnu4HI GCNGC 4 cut(s) 31, 315, 428, 854
FokI GGATG 4 cut(s) 215, 685, 850, 1012
FriOI GRGCYC 2 cut(s) 89, 490
Fsp4HI GCNGC 4 cut(s) 31, 315, 428, 854
FspBI CTAG 9 cut(s) 219, 395, 504, 512, 570, 581, 810, 1041, 1129
GlaI GCGC 3 cut(s) 16, 318, 1110
GluI GCNGC 4 cut(s) 31, 315, 428, 854
HaeIII GGCC 4 cut(s) 376, 414, 742, 1081
HapII CCGG 2 cut(s) 548, 957
HhaI GCGC 3 cut(s) 17, 319, 1111
Hin1II CATG 5 cut(s) 289, 484, 625, 920, 1104
Hin6I GCGC 3 cut(s) 15, 317, 1109
HinP1I GCGC 3 cut(s) 15, 317, 1109
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 2 cut(s) 44, 237
HinfI GANTC 6 cut(s) 53, 98, 432, 759, 767, 797
HpaII CCGG 2 cut(s) 548, 957
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 2 cut(s) 169, 984
Hpy188I TCNGA 6 cut(s) 389, 544, 663, 684, 834, 875
Hpy188III TCNNGA 3 cut(s) 402, 754, 847
Hpy8I GTNNAC 2 cut(s) 169, 984
HpyAV CCTTC 7 cut(s) 319, 387, 404, 587, 658, 933, 1042
HpyCH4V TGCA 7 cut(s) 33, 106, 128, 337, 625, 631, 728
HpyF10VI GCNNNNNNNGC 8 cut(s) 93, 299, 424, 500, 949, 1026, 1087, 1106
HpyF3I CTNAG 3 cut(s) 420, 784, 831
Hsp92II CATG 5 cut(s) 289, 484, 625, 920, 1104
HspAI GCGC 3 cut(s) 15, 317, 1109
Ksp22I TGATCA 1 cut(s) 384
Kzo9I GATC 3 cut(s) 384, 451, 843
LmnI GCTCC 2 cut(s) 358, 544
LpnPI CCDG 8 cut(s) 19, 462, 476, 561, 944, 970, 1063, 1076
Lsp1109I GCAGC 4 cut(s) 17, 326, 414, 865
LweI GCATC 6 cut(s) 86, 407, 627, 715, 993, 1103
MaeI CTAG 9 cut(s) 219, 395, 504, 512, 570, 581, 810, 1041, 1129
MaeIII GTNAC 1 cut(s) 211
MalI GATC 3 cut(s) 386, 453, 845
MboI GATC 3 cut(s) 384, 451, 843
MboII GAAGA 9 cut(s) 149, 341, 359, 461, 665, 697, 764, 853, 1113
MhlI GDGCHC 3 cut(s) 89, 363, 490
MluCI AATT 7 cut(s) 69, 527, 532, 669, 689, 789, 944
MlyI GAGTC 4 cut(s) 47, 92, 776, 791
MmeI TCCRAC 1 cut(s) 213
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 3 cut(s) 252, 531, 899
MslI CAYNNNNRTG 2 cut(s) 636, 1099
MspI CCGG 2 cut(s) 548, 957
MspR9I CCNGG 1 cut(s) 958
MvnI CGCG 2 cut(s) 820, 863
MwoI GCNNNNNNNGC 8 cut(s) 93, 299, 424, 500, 949, 1026, 1087, 1106
NciI CCSGG 1 cut(s) 958
NdeII GATC 3 cut(s) 384, 451, 843
NheI GCTAGC 2 cut(s) 503, 809
NlaIII CATG 5 cut(s) 289, 484, 625, 920, 1104
NlaIV GGNNCC 1 cut(s) 546
NmeAIII GCCGAG 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 211
PaeR7I CTCGAG 2 cut(s) 764, 803
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 801
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 432, 759
PkrI GCNGC 4 cut(s) 32, 316, 429, 855
PleI GAGTC 4 cut(s) 47, 92, 775, 791
PpsI GAGTC 4 cut(s) 47, 92, 775, 791
Psp124BI GAGCTC 1 cut(s) 490
PspN4I GGNNCC 1 cut(s) 546
PspPI GGNCC 3 cut(s) 413, 695, 740
PspXI VCTCGAGB 2 cut(s) 764, 803
PstI CTGCAG 1 cut(s) 35
RsaI GTAC 1 cut(s) 985
RsaNI GTAC 1 cut(s) 984
RseI CAYNNNNRTG 2 cut(s) 636, 1099
SacI GAGCTC 1 cut(s) 490
SaqAI TTAA 3 cut(s) 252, 531, 899
SatI GCNGC 4 cut(s) 31, 315, 428, 854
Sau3AI GATC 3 cut(s) 384, 451, 843
Sau96I GGNCC 3 cut(s) 413, 695, 740
SchI GAGTC 4 cut(s) 47, 92, 776, 791
ScrFI CCNGG 1 cut(s) 958
SduI GDGCHC 3 cut(s) 89, 363, 490
SfaNI GCATC 6 cut(s) 86, 407, 627, 715, 993, 1103
SfcI CTRYAG 1 cut(s) 31
Sfr274I CTCGAG 2 cut(s) 764, 803
SinI GGWCC 1 cut(s) 695
SlaI CTCGAG 2 cut(s) 764, 803
SmiMI CAYNNNNRTG 2 cut(s) 636, 1099
SmlI CTYRAG 3 cut(s) 586, 764, 803
SmoI CTYRAG 3 cut(s) 586, 764, 803
Sse9I AATT 7 cut(s) 69, 527, 532, 669, 689, 789, 944
SsiI CCGC 4 cut(s) 416, 494, 818, 863
SspMI CTAG 9 cut(s) 219, 395, 504, 512, 570, 581, 810, 1041, 1129
SstI GAGCTC 1 cut(s) 490
StyD4I CCNGG 1 cut(s) 956
StyI CCWWGG 1 cut(s) 89
TaqI TCGA 5 cut(s) 401, 765, 795, 804, 846
TasI AATT 7 cut(s) 69, 527, 532, 669, 689, 789, 944
TatI WGTACW 1 cut(s) 983
TfiI GAWTC 2 cut(s) 432, 759
Tru1I TTAA 3 cut(s) 252, 531, 899
Tru9I TTAA 3 cut(s) 252, 531, 899
TscAI CASTG 3 cut(s) 216, 377, 938
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 4 cut(s) 30, 314, 427, 853
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 4 cut(s) 105, 274, 312, 854
TspGWI ACGGA 1 cut(s) 691
TspRI CASTG 3 cut(s) 216, 377, 938
VpaK11BI GGWCC 1 cut(s) 695
XapI RAATTY 2 cut(s) 69, 527
XhoI CTCGAG 2 cut(s) 764, 803
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 9 cut(s) 219, 395, 504, 512, 570, 581, 810, 1041, 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.