RLG00000032776

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017624
Physical Location & Seq
Reverse (-)
18955479 .. 18957098
1620 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032776

Sequence Viewer

Length: 1620 bp
ATGGAAGACTTCAATGCTTGGCTCTCCTTCCCAATCCATGGGAGTCTCAACCATGATCTCGCTATTAGAAGGTTCTGTCCAGCAAGACTTGAGCAAGAACAAGGAGGAGGAGACTGGGAGGAGATACTGAATGAGATTCAATTTTGTTCACCTTCTGATGATTCAACTAGCACTCCTTGCTTGCCTGCTTCCGAAGTTGATGAGTTTGTTGACAGCTTCATCAACATGGATCAATGCAAGGATATCGACGACGATTATCAGTCCCTGGAGAAACAACAAAGTAGTTTTGATTACTTCCAAGATGGGATCCAGACATTTTCAATGGTGGATGATCTATATGGAGATGTCATGATGATGATGGAAGGAGATGATCAGATGGAGATGTGTGGCTCAGTTGAGGATTTCGGAGTTGTAGATGATCAAACTACTATGGTGCCTAGCGTTGAAGAGGTGAGCCATGGTGTGGACCAAGGGCTTCACTTGGTGCACATGTTACTGGCTTGTGCTGAAGCTGTGGGGTGCAGAGATACAAGGCTTGCAGATTCATTGCTCACCCAAATCTGGGCTTCGGTTAGTCCTTTCGGTGATTCCTTGCAGAGAGTCTCATACTGCTTTGCAATGGGTTTGAAGTCTAGGCTCTCTCTTCTTCACAATGTGAACCTAAATGGTACATTCAACAATGATACTATGGATGTGTCGCTGATCGCTAGGGAGGAGAAATTGGAGGCTTTTCAACTCCTTCACCAGACGACTCCTTACATAGCTTTCGGTTTCATGGTTGCGAATGAAGCTATTTGTCAAGCAGCGCAAGGGAAGGAGAGCTTGCACATCATTGATTTGGGAATGGAGCACACTCTTCAATGGCCATCCTTGTTAAGAACCCTGGCATCAAGACCCGAAGGCCCACCTAAGAAGCTCCGTATCACCGGACTAGTTGAAGATCACAATGTCTTAGAGCTTGACGCTGGCATGAGAGTCCTGGTGGAAGAGGCTTGTTCACTTGGCATACCAATAGAATTCAACATGATCTCAGAGCCGGTAAAACCATCGCTTCTGACTAAAGAGAATCTAGACTTGAGAGAAGGGGAAGCATTATGTGTCAATAGCATAATGCATCTGCATATGTATGTCAAAGAGAGCAGAGGATCTTTGAAGGCAATCCTCCAAGCCTTAAAGAAGTTAGGTCCAAGTGTGCTTACATTGGTAGAGCAAGATGCAAATCACAACGGGCCGTTTTTTCTTGGGAGATTTCTCGAGTCTCTTCACTTTTATTCTGCAATTTTTGACTCTCTCGAGGTGAGCCTCCCACGAAACAGCCCTCAGAGGATGAAAATAGAGAGACTTCACTTCGCAGATGAAATTCGGAACATTGTTGCGTTTGAGGGCTTAAATAGGGTTGAGAGGCATGAAAGGGCAGACCAATGGAGAAGGCAATTAGGCAGAGCTGGGTTTCAAGTTATGGGATTGAAGTGTACAAGTCAGGCTAGGATGATGCTATCTGTTTATGGCTGTGATGGGTACAGTTTGGCCAGTGAGAAGGGTTGTCTCCTCCTTGGGTGGAAAGGAAGGCCCATAATGTTGGCAGCCGCATGGCAAGTGAACAATGCCTCTAACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

540

Amino Acids

60.44

Weight (kDa)

4.78

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 161 - 532 3.1e-105 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g14680
malus_domestica MD05G1229500.v1.1
prunus_persica Prupe.4G132100_v2.0.a1
pyrus_communis pycom05g21040
rosa_chinensis RchiOBHm_Chr5g0024171
rosa_laevigata RLG00000032776
rosa_multiflora Rmu_sc0004033.1_g000010
rosa_rugosa Rorug05G0082200
rosa_samantha Rh5BG169400 Rh5CG185300 Rh5DG171700
rosa_wichuraiana Rw5G015590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 433
AccB7I CCANNNNNTGG 2 cut(s) 38, 463
AciI CCGC 1 cut(s) 1587
AclWI GGATC 4 cut(s) 237, 301, 314, 1153
AcoI YGGCCR 2 cut(s) 863, 1527
AcsI RAATTY 2 cut(s) 1016, 1359
AcuI CTGAAG 1 cut(s) 528
AdeI CACNNNGTG 2 cut(s) 484, 655
AfaI GTAC 3 cut(s) 670, 1474, 1520
AfiI CCNNNNNNNGG 4 cut(s) 38, 463, 561, 562
AflIII ACRYGT 1 cut(s) 489
AhlI ACTAGT 1 cut(s) 931
AjnI CCWGG 3 cut(s) 264, 882, 978
AluBI AGCT 8 cut(s) 216, 512, 764, 791, 822, 916, 958, 1445
AluI AGCT 8 cut(s) 216, 512, 764, 791, 822, 916, 958, 1445
Alw21I GWGCWC 2 cut(s) 489, 852
Alw26I GTCTC 6 cut(s) 50, 105, 607, 1263, 1333, 1550
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 4 cut(s) 237, 301, 314, 1153
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 2 cut(s) 1253, 1292
AoxI GGCC 5 cut(s) 863, 901, 1229, 1527, 1568
ApaLI GTGCAC 1 cut(s) 485
ApeKI GCWGC 2 cut(s) 803, 1583
ApoI RAATTY 2 cut(s) 1016, 1359
AspLEI GCGC 1 cut(s) 808
AspS9I GGNCC 5 cut(s) 466, 902, 1184, 1229, 1569
AsuHPI GGTGA 7 cut(s) 141, 463, 544, 596, 734, 916, 1309
AvaI CYCGRG 2 cut(s) 1253, 1292
AvaII GGWCC 2 cut(s) 466, 1184
BaeGI GKGCMC 1 cut(s) 489
BalI TGGCCA 2 cut(s) 865, 1529
BamHI GGATCC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 433
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 489, 852
BbvI GCAGC 2 cut(s) 815, 1595
BccI CCATC 6 cut(s) 296, 352, 370, 874, 1054, 1508
BceAI ACGGC 1 cut(s) 1216
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 3 cut(s) 266, 884, 980
BclI TGATCA 2 cut(s) 370, 418
BcoDI GTCTC 6 cut(s) 50, 105, 607, 1263, 1333, 1550
BcuI ACTAGT 1 cut(s) 931
BfaI CTAG 7 cut(s) 168, 438, 633, 708, 932, 1070, 1485
BisI GCNGC 3 cut(s) 804, 1584, 1587
BlsI GCNGC 3 cut(s) 805, 1585, 1588
Bme1390I CCNGG 3 cut(s) 266, 884, 980
Bme18I GGWCC 2 cut(s) 466, 1184
BmeT110I CYCGRG 2 cut(s) 1253, 1292
BmgT120I GGNCC 5 cut(s) 466, 902, 1184, 1229, 1569
BmiI GGNNCC 2 cut(s) 308, 435
BmrFI CCNGG 3 cut(s) 266, 884, 980
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 4 cut(s) 896, 1123, 1204, 1482
BmuI ACTGGG 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 110, 1096
BsaBI GATNNNNATC 1 cut(s) 1218
BsaJI CCNNGG 6 cut(s) 37, 264, 457, 469, 882, 1552
BsaWI WCCGGW 1 cut(s) 926
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 4 cut(s) 38, 463, 561, 562
Bse118I RCCGGY 1 cut(s) 1036
Bse1I ACTGG 3 cut(s) 119, 501, 1530
Bse3DI GCAATG 2 cut(s) 545, 624
Bse8I GATNNNNATC 1 cut(s) 1218
BseBI CCWGG 3 cut(s) 266, 884, 980
BseDI CCNNGG 6 cut(s) 37, 264, 457, 469, 882, 1552
BseGI GGATG 5 cut(s) 334, 697, 866, 1332, 1494
BseJI GATNNNNATC 1 cut(s) 1218
BseLI CCNNNNNNNGG 4 cut(s) 38, 463, 561, 562
BseMI GCAATG 2 cut(s) 545, 624
BseMII CTCAG 3 cut(s) 405, 1044, 1334
BseNI ACTGG 3 cut(s) 119, 501, 1530
BseRI GAGGAG 5 cut(s) 120, 123, 134, 728, 1538
BseSI GKGCMC 1 cut(s) 489
BseXI GCAGC 2 cut(s) 815, 1595
BseYI CCCAGC 1 cut(s) 1445
BsgI GTGCAG 1 cut(s) 541
BshFI GGCC 5 cut(s) 865, 903, 1231, 1529, 1570
BshNI GGYRCC 1 cut(s) 433
BsiHKAI GWGCWC 2 cut(s) 489, 852
BsiHKCI CYCGRG 2 cut(s) 1253, 1292
BsiSI CCGG 2 cut(s) 927, 1037
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 4 cut(s) 38, 463, 561, 562
BsmAI GTCTC 6 cut(s) 50, 105, 607, 1263, 1333, 1550
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 5 cut(s) 865, 903, 1231, 1529, 1570
BsoBI CYCGRG 2 cut(s) 1253, 1292
Bsp1286I GDGCHC 2 cut(s) 489, 852
Bsp1407I TGTACA 1 cut(s) 1472
Bsp19I CCATGG 2 cut(s) 37, 457
BspACI CCGC 1 cut(s) 1587
BspANI GGCC 5 cut(s) 865, 903, 1231, 1529, 1570
BspCNI CTCAG 3 cut(s) 404, 1043, 1333
BspHI TCATGA 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 308, 435
BspPI GGATC 4 cut(s) 237, 301, 314, 1153
BspT107I GGYRCC 1 cut(s) 433
BsrDI GCAATG 2 cut(s) 545, 624
BsrFI RCCGGY 1 cut(s) 1036
BsrGI TGTACA 1 cut(s) 1472
BsrI ACTGG 3 cut(s) 119, 501, 1530
BssAI RCCGGY 1 cut(s) 1036
BssECI CCNNGG 6 cut(s) 37, 264, 457, 469, 882, 1552
BssT1I CCWWGG 4 cut(s) 37, 457, 469, 1552
Bst2UI CCWGG 3 cut(s) 266, 884, 980
Bst4CI ACNGT 1 cut(s) 1523
Bst6I CTCTTC 5 cut(s) 441, 648, 861, 981, 1266
BstAPI GCANNNNNTGC 1 cut(s) 177
BstAUI TGTACA 1 cut(s) 1472
BstC8I GCNNGC 5 cut(s) 182, 186, 537, 824, 967
BstDEI CTNAG 6 cut(s) 391, 909, 952, 1030, 1320, 1617
BstDSI CCRYGG 2 cut(s) 37, 457
BstF5I GGATG 5 cut(s) 334, 697, 866, 1332, 1494
BstHHI GCGC 1 cut(s) 808
BstMAI GTCTC 6 cut(s) 50, 105, 607, 1263, 1333, 1550
BstMWI GCNNNNNNNGC 2 cut(s) 177, 788
BstNI CCWGG 3 cut(s) 266, 884, 980
BstNSI RCATGY 1 cut(s) 493
BstSCI CCNGG 3 cut(s) 264, 882, 978
BstSLI GKGCMC 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 815, 1595
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 2 cut(s) 306, 1145
BstXI CCANNNNNNTGG 1 cut(s) 1579
BstYI RGATCY 2 cut(s) 306, 1145
BsuRI GGCC 5 cut(s) 865, 903, 1231, 1529, 1570
BtgI CCRYGG 2 cut(s) 37, 457
BtgZI GCGATG 1 cut(s) 1032
BtsCI GGATG 5 cut(s) 334, 697, 866, 1332, 1494
BtsIMutI CAGTG 1 cut(s) 1537
Cac8I GCNNGC 5 cut(s) 182, 186, 537, 824, 967
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 348
CfoI GCGC 1 cut(s) 808
Cfr10I RCCGGY 1 cut(s) 1036
Cfr13I GGNCC 5 cut(s) 466, 902, 1184, 1229, 1569
CseI GACGC 1 cut(s) 971
Csp6I GTAC 3 cut(s) 669, 1473, 1519
CviQI GTAC 3 cut(s) 669, 1473, 1519
DdeI CTNAG 6 cut(s) 391, 909, 952, 1030, 1320, 1617
DraIII CACNNNGTG 2 cut(s) 484, 655
EaeI YGGCCR 2 cut(s) 863, 1527
Eam1104I CTCTTC 5 cut(s) 441, 648, 861, 981, 1266
EarI CTCTTC 5 cut(s) 441, 648, 861, 981, 1266
Eco130I CCWWGG 4 cut(s) 37, 457, 469, 1552
Eco32I GATATC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 466, 1184
Eco57I CTGAAG 1 cut(s) 528
Eco88I CYCGRG 2 cut(s) 1253, 1292
EcoRI GAATTC 1 cut(s) 1016
EcoRII CCWGG 3 cut(s) 264, 882, 978
EcoRV GATATC 1 cut(s) 244
EcoT14I CCWWGG 4 cut(s) 37, 457, 469, 1552
EcoT22I ATGCAT 1 cut(s) 1116
ErhI CCWWGG 4 cut(s) 37, 457, 469, 1552
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FaqI GGGAC 1 cut(s) 247
FauNDI CATATG 1 cut(s) 1122
FbaI TGATCA 2 cut(s) 370, 418
Fnu4HI GCNGC 3 cut(s) 804, 1584, 1587
FokI GGATG 5 cut(s) 341, 704, 853, 1339, 1501
Fsp4HI GCNGC 3 cut(s) 804, 1584, 1587
FspBI CTAG 7 cut(s) 168, 438, 633, 708, 932, 1070, 1485
GlaI GCGC 1 cut(s) 807
GluI GCNGC 3 cut(s) 804, 1584, 1587
GsaI CCCAGC 1 cut(s) 1449
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 5 cut(s) 865, 903, 1231, 1529, 1570
HapII CCGG 2 cut(s) 927, 1037
HgaI GACGC 1 cut(s) 971
HhaI GCGC 1 cut(s) 808
Hin6I GCGC 1 cut(s) 806
HinP1I GCGC 1 cut(s) 806
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HpaII CCGG 2 cut(s) 927, 1037
HphI GGTGA 7 cut(s) 141, 463, 544, 596, 734, 916, 1309
Hpy166II GTNNAC 8 cut(s) 149, 211, 466, 487, 658, 998, 1473, 1600
Hpy188I TCNGA 8 cut(s) 157, 193, 375, 407, 1033, 1056, 1323, 1365
Hpy188III TCNNGA 6 cut(s) 310, 349, 891, 1070, 1253, 1292
Hpy8I GTNNAC 8 cut(s) 149, 211, 466, 487, 658, 998, 1473, 1600
Hpy99I CGWCG 2 cut(s) 251, 254
HpyCH4III ACNGT 1 cut(s) 1523
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 788
HpyF3I CTNAG 6 cut(s) 391, 909, 952, 1030, 1320, 1617
HspAI GCGC 1 cut(s) 806
Ksp22I TGATCA 2 cut(s) 370, 418
LmnI GCTCC 2 cut(s) 847, 921
Lsp1109I GCAGC 2 cut(s) 815, 1595
LweI GCATC 4 cut(s) 896, 1123, 1204, 1482
MaeI CTAG 7 cut(s) 168, 438, 633, 708, 932, 1070, 1485
MaeIII GTNAC 1 cut(s) 492
MboII GAAGA 8 cut(s) 17, 458, 635, 638, 848, 950, 998, 1253
MflI RGATCY 2 cut(s) 306, 1145
MhlI GDGCHC 2 cut(s) 489, 852
MlsI TGGCCA 2 cut(s) 865, 1529
MluCI AATT 6 cut(s) 140, 719, 1016, 1278, 1359, 1433
MluNI TGGCCA 2 cut(s) 865, 1529
MlyI GAGTC 6 cut(s) 52, 609, 745, 984, 1265, 1280
Mox20I TGGCCA 2 cut(s) 865, 1529
Mph1103I ATGCAT 1 cut(s) 1116
MscI TGGCCA 2 cut(s) 865, 1529
MseI TTAA 3 cut(s) 875, 1172, 1388
MslI CAYNNNNRTG 3 cut(s) 224, 353, 1125
Msp20I TGGCCA 2 cut(s) 865, 1529
MspI CCGG 2 cut(s) 927, 1037
MspR9I CCNGG 3 cut(s) 266, 884, 980
MvaI CCWGG 3 cut(s) 266, 884, 980
MwoI GCNNNNNNNGC 2 cut(s) 177, 788
NcoI CCATGG 2 cut(s) 37, 457
NdeI CATATG 1 cut(s) 1122
NlaIV GGNNCC 2 cut(s) 308, 435
NsiI ATGCAT 1 cut(s) 1116
NspI RCATGY 1 cut(s) 493
PaeR7I CTCGAG 2 cut(s) 1253, 1292
PagI TCATGA 1 cut(s) 348
PciI ACATGT 1 cut(s) 489
PfeI GAWTC 5 cut(s) 136, 161, 542, 587, 1066
PflMI CCANNNNNTGG 2 cut(s) 38, 463
PkrI GCNGC 3 cut(s) 805, 1585, 1588
PleI GAGTC 6 cut(s) 51, 608, 745, 983, 1264, 1280
PpsI GAGTC 6 cut(s) 51, 608, 745, 983, 1264, 1280
PscI ACATGT 1 cut(s) 489
Psp6I CCWGG 3 cut(s) 264, 882, 978
PspFI CCCAGC 1 cut(s) 1445
PspGI CCWGG 3 cut(s) 264, 882, 978
PspN4I GGNNCC 2 cut(s) 308, 435
PspPI GGNCC 5 cut(s) 466, 902, 1184, 1229, 1569
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 2 cut(s) 306, 1145
RsaI GTAC 3 cut(s) 670, 1474, 1520
RsaNI GTAC 3 cut(s) 669, 1473, 1519
RseI CAYNNNNRTG 3 cut(s) 224, 353, 1125
SaqAI TTAA 3 cut(s) 875, 1172, 1388
SatI GCNGC 3 cut(s) 804, 1584, 1587
Sau96I GGNCC 5 cut(s) 466, 902, 1184, 1229, 1569
SchI GAGTC 6 cut(s) 52, 609, 745, 984, 1265, 1280
ScrFI CCNGG 3 cut(s) 266, 884, 980
SduI GDGCHC 2 cut(s) 489, 852
SfaNI GCATC 4 cut(s) 896, 1123, 1204, 1482
Sfr274I CTCGAG 2 cut(s) 1253, 1292
SinI GGWCC 2 cut(s) 466, 1184
SlaI CTCGAG 2 cut(s) 1253, 1292
SmiMI CAYNNNNRTG 3 cut(s) 224, 353, 1125
SmlI CTYRAG 4 cut(s) 89, 1075, 1253, 1292
SmoI CTYRAG 4 cut(s) 89, 1075, 1253, 1292
SpeI ACTAGT 1 cut(s) 931
Sse9I AATT 6 cut(s) 140, 719, 1016, 1278, 1359, 1433
SsiI CCGC 1 cut(s) 1587
SspMI CTAG 7 cut(s) 168, 438, 633, 708, 932, 1070, 1485
StyD4I CCNGG 3 cut(s) 264, 882, 978
StyI CCWWGG 4 cut(s) 37, 457, 469, 1552
TaaI ACNGT 1 cut(s) 1523
TaqI TCGA 3 cut(s) 246, 1254, 1293
TasI AATT 6 cut(s) 140, 719, 1016, 1278, 1359, 1433
TatI WGTACW 1 cut(s) 1472
TauI GCSGC 1 cut(s) 1589
TfiI GAWTC 5 cut(s) 136, 161, 542, 587, 1066
Tru1I TTAA 3 cut(s) 875, 1172, 1388
Tru9I TTAA 3 cut(s) 875, 1172, 1388
TscAI CASTG 1 cut(s) 1537
TseI GCWGC 2 cut(s) 803, 1583
TspDTI ATGAA 7 cut(s) 208, 534, 763, 801, 1343, 1371, 1422
TspGWI ACGGA 1 cut(s) 908
TspRI CASTG 1 cut(s) 1537
Van91I CCANNNNNTGG 2 cut(s) 38, 463
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 466, 1184
XapI RAATTY 2 cut(s) 1016, 1359
XbaI TCTAGA 1 cut(s) 1069
XceI RCATGY 1 cut(s) 493
XhoI CTCGAG 2 cut(s) 1253, 1292
XspI CTAG 7 cut(s) 168, 438, 633, 708, 932, 1070, 1485
Zsp2I ATGCAT 1 cut(s) 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.