Rh5CG185300

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017624
Physical Location & Seq
Reverse (-)
18527483 .. 18528673
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG185300.1

Sequence Viewer

Length: 1191 bp
ATGGTGCCTAGTGTTGAAGCGGTGAGCCATGGTGTGGACCAAGGGCTTCACTTGGTGCACATGTTACTGGCTTGTGCTGAAGCTGTGGGGTGCAGAGATACAAGGCTTGCAGATTCATTGCTCACCCAAATCTGGGCTTCGGTTAGTCCATTCGGTGATTCCTTGCAGAGAGTCTCATACTGCTTTTCAATGGGGTTGAAGTCTAGGCTCTCTCTTCTTCACAATGTGAACCTAAATGGTACATTCAACAATGATACTATGGATGTGTCGCTGATCGCTAGGGAGGAGAAATTGGAGGCTTTTCAACTCCTTCACCAGACGACTCCTTACATAGCTTTCGGTTTCATGGTTGCGAATGAAGCTATTTGTCAAGCAGCACAAGGGAAGGAGAGCTTGCACATCATTGATTTGGGAATGGAGCACACTCTTCAATGGCCATCCTTGTTAAGAACCCTGGCATCAAGACCCGAAGGCCCACCTAAGAAGCTCCGTATCACCGGTCTAGTTGAAGATCACAATGTCTTAGAGCTTGACGCTGGCATGAGAGTCCTGGTGGAAGAGGCTCGTTCACTTGGCATACCAATAGAATTCAACATGATCTCAGAGCCGGTAAAACCATCACTTCTGACTAAAGAGAATCTAGACTTGAGAGAAGGGGAAGCATTATGTGTCAATAGCATAATGCACCTGCATATGTATGTCAAAGAGAGCAGAGGATCTTTGAAGGCAATCCTCCAAGCCTTCAAGAAGTTAGGTCCAAGTGTGCTTACATTGGTAGAGCAAGATGCAAATCACAACGGGCCGTTTTTTCTTGGGAGATTTCTCGAGTCTCTTCACTTTTATTCTGCAATTTTTGACTCTCTCGAGGTGAGCCTTCCACGAAACAGCCCTCAGAGGATGAAAATAGAGAGACTTCACTTTGCAGATGAAATTCGGAACATTGTTGCGTTTGAGGGATTAAATAGGGTTGAGAGGCATGAAAGGGCAGACCAATGGAGAAGGCAATTAGGCAGAGCTGGGTTTCAAGTTATGGGATTGAAGTGTACAAGTCAGGCTAGGATGATGCTATCTGTTTATGGCTGTGATGGGTACAGTTTGGCTAGTGAGAAGGGTTGTCTCCTCCTAGGGTGGAAAGGAAGGCCTATAATGTTGGCATCTGCATGGCAAGTGAACAATGCCTCTAACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

396

Amino Acids

44.21

Weight (kDa)

6.54

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 18 - 389 4.8e-107 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g14680
malus_domestica MD05G1229500.v1.1
prunus_persica Prupe.4G132100_v2.0.a1
pyrus_communis pycom05g21040
rosa_chinensis RchiOBHm_Chr5g0024171
rosa_laevigata RLG00000032776
rosa_multiflora Rmu_sc0004033.1_g000010
rosa_rugosa Rorug05G0082200
rosa_samantha Rh5BG169400 Rh5CG185300 Rh5DG171700
rosa_wichuraiana Rw5G015590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 696
Acc36I ACCTGC 1 cut(s) 696
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 34
AciI CCGC 1 cut(s) 20
AclWI GGATC 1 cut(s) 724
AcoI YGGCCR 1 cut(s) 434
AcsI RAATTY 2 cut(s) 587, 930
AcuI CTGAAG 1 cut(s) 99
AdeI CACNNNGTG 2 cut(s) 55, 226
AfaI GTAC 3 cut(s) 241, 1045, 1091
AfiI CCNNNNNNNGG 3 cut(s) 34, 132, 133
AflIII ACRYGT 1 cut(s) 60
AgeI ACCGGT 1 cut(s) 497
AjnI CCWGG 2 cut(s) 453, 549
AluBI AGCT 7 cut(s) 83, 335, 362, 393, 487, 529, 1016
AluI AGCT 7 cut(s) 83, 335, 362, 393, 487, 529, 1016
Alw21I GWGCWC 2 cut(s) 60, 423
Alw26I GTCTC 4 cut(s) 178, 834, 904, 1121
Alw44I GTGCAC 1 cut(s) 56
AlwI GGATC 1 cut(s) 724
Ama87I CYCGRG 2 cut(s) 824, 863
AoxI GGCC 4 cut(s) 434, 472, 800, 1139
ApaLI GTGCAC 1 cut(s) 56
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 2 cut(s) 587, 930
AsiGI ACCGGT 1 cut(s) 497
AspA2I CCTAGG 1 cut(s) 1123
AspS9I GGNCC 4 cut(s) 37, 473, 755, 800
AsuHPI GGTGA 6 cut(s) 34, 115, 167, 305, 487, 880
AvaI CYCGRG 2 cut(s) 824, 863
AvaII GGWCC 2 cut(s) 37, 755
AvrII CCTAGG 1 cut(s) 1123
BaeGI GKGCMC 1 cut(s) 60
BalI TGGCCA 1 cut(s) 436
BanI GGYRCC 1 cut(s) 4
Bbv12I GWGCWC 2 cut(s) 60, 423
BbvI GCAGC 1 cut(s) 386
BccI CCATC 3 cut(s) 445, 625, 1079
BceAI ACGGC 1 cut(s) 787
BciT130I CCWGG 2 cut(s) 455, 551
BcoDI GTCTC 4 cut(s) 178, 834, 904, 1121
BfaI CTAG 8 cut(s) 9, 204, 279, 503, 641, 1056, 1101, 1124
BfuAI ACCTGC 1 cut(s) 696
BisI GCNGC 1 cut(s) 375
BlnI CCTAGG 1 cut(s) 1123
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 2 cut(s) 455, 551
Bme18I GGWCC 2 cut(s) 37, 755
BmeT110I CYCGRG 2 cut(s) 824, 863
BmgT120I GGNCC 4 cut(s) 37, 473, 755, 800
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 455, 551
BmsI GCATC 4 cut(s) 467, 775, 1053, 1163
BpuEI CTTGAG 1 cut(s) 667
BsaBI GATNNNNATC 1 cut(s) 789
BsaJI CCNNGG 4 cut(s) 28, 40, 453, 1123
BsaWI WCCGGW 1 cut(s) 497
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 132, 133
Bse118I RCCGGY 2 cut(s) 497, 607
Bse1I ACTGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 116
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 2 cut(s) 455, 551
BseDI CCNNGG 4 cut(s) 28, 40, 453, 1123
BseGI GGATG 4 cut(s) 268, 437, 903, 1065
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 3 cut(s) 34, 132, 133
BseMI GCAATG 1 cut(s) 116
BseMII CTCAG 2 cut(s) 615, 905
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 2 cut(s) 299, 1109
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 1 cut(s) 386
BseYI CCCAGC 1 cut(s) 1016
BsgI GTGCAG 1 cut(s) 112
BshFI GGCC 4 cut(s) 436, 474, 802, 1141
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 497
BsiHKAI GWGCWC 2 cut(s) 60, 423
BsiHKCI CYCGRG 2 cut(s) 824, 863
BsiSI CCGG 2 cut(s) 498, 608
BslI CCNNNNNNNGG 3 cut(s) 34, 132, 133
BsmAI GTCTC 4 cut(s) 178, 834, 904, 1121
BsnI GGCC 4 cut(s) 436, 474, 802, 1141
BsoBI CYCGRG 2 cut(s) 824, 863
Bsp1286I GDGCHC 2 cut(s) 60, 423
Bsp1407I TGTACA 1 cut(s) 1043
Bsp143I GATC 4 cut(s) 273, 511, 597, 716
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 1 cut(s) 20
BspANI GGCC 4 cut(s) 436, 474, 802, 1141
BspCNI CTCAG 2 cut(s) 614, 904
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 696
BspPI GGATC 1 cut(s) 724
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 116
BsrFI RCCGGY 2 cut(s) 497, 607
BsrGI TGTACA 1 cut(s) 1043
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 2 cut(s) 497, 607
BssECI CCNNGG 4 cut(s) 28, 40, 453, 1123
BssMI GATC 4 cut(s) 273, 511, 597, 716
BssT1I CCWWGG 3 cut(s) 28, 40, 1123
Bst2UI CCWGG 2 cut(s) 455, 551
Bst4CI ACNGT 1 cut(s) 1094
Bst6I CTCTTC 4 cut(s) 219, 432, 552, 837
BstAUI TGTACA 1 cut(s) 1043
BstC8I GCNNGC 3 cut(s) 108, 395, 538
BstDEI CTNAG 5 cut(s) 480, 523, 601, 891, 1188
BstDSI CCRYGG 1 cut(s) 28
BstF5I GGATG 4 cut(s) 268, 437, 903, 1065
BstKTI GATC 4 cut(s) 276, 514, 600, 719
BstMAI GTCTC 4 cut(s) 178, 834, 904, 1121
BstMBI GATC 4 cut(s) 273, 511, 597, 716
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 2 cut(s) 455, 551
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 2 cut(s) 453, 549
BstSLI GKGCMC 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 386
BstX2I RGATCY 1 cut(s) 716
BstYI RGATCY 1 cut(s) 716
BsuRI GGCC 4 cut(s) 436, 474, 802, 1141
BtgI CCRYGG 1 cut(s) 28
BtsCI GGATG 4 cut(s) 268, 437, 903, 1065
BveI ACCTGC 1 cut(s) 696
Cac8I GCNNGC 3 cut(s) 108, 395, 538
Cfr10I RCCGGY 2 cut(s) 497, 607
Cfr13I GGNCC 4 cut(s) 37, 473, 755, 800
CseI GACGC 1 cut(s) 542
Csp6I GTAC 3 cut(s) 240, 1044, 1090
CspAI ACCGGT 1 cut(s) 497
CviAII CATG 7 cut(s) 29, 61, 346, 541, 595, 977, 1161
CviQI GTAC 3 cut(s) 240, 1044, 1090
DdeI CTNAG 5 cut(s) 480, 523, 601, 891, 1188
DpnI GATC 4 cut(s) 275, 513, 599, 718
DpnII GATC 4 cut(s) 273, 511, 597, 716
DraIII CACNNNGTG 2 cut(s) 55, 226
EaeI YGGCCR 1 cut(s) 434
Eam1104I CTCTTC 4 cut(s) 219, 432, 552, 837
EarI CTCTTC 4 cut(s) 219, 432, 552, 837
Eco130I CCWWGG 3 cut(s) 28, 40, 1123
Eco147I AGGCCT 1 cut(s) 1141
Eco47I GGWCC 2 cut(s) 37, 755
Eco57I CTGAAG 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 824, 863
EcoRI GAATTC 1 cut(s) 587
EcoRII CCWGG 2 cut(s) 453, 549
EcoT14I CCWWGG 3 cut(s) 28, 40, 1123
ErhI CCWWGG 3 cut(s) 28, 40, 1123
FaeI CATG 7 cut(s) 32, 64, 349, 544, 598, 980, 1164
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FatI CATG 7 cut(s) 28, 60, 345, 540, 594, 976, 1160
FauNDI CATATG 1 cut(s) 693
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 4 cut(s) 275, 424, 910, 1072
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 8 cut(s) 9, 204, 279, 503, 641, 1056, 1101, 1124
GluI GCNGC 1 cut(s) 375
GsaI CCCAGC 1 cut(s) 1020
HaeIII GGCC 4 cut(s) 436, 474, 802, 1141
HapII CCGG 2 cut(s) 498, 608
HgaI GACGC 1 cut(s) 542
Hin1II CATG 7 cut(s) 32, 64, 349, 544, 598, 980, 1164
HinfI GANTC 8 cut(s) 113, 158, 171, 322, 546, 637, 827, 857
HpaII CCGG 2 cut(s) 498, 608
HphI GGTGA 6 cut(s) 34, 115, 167, 305, 487, 880
Hpy166II GTNNAC 6 cut(s) 37, 58, 229, 569, 1044, 1171
Hpy188I TCNGA 4 cut(s) 604, 627, 894, 936
Hpy188III TCNNGA 5 cut(s) 462, 641, 745, 824, 863
Hpy8I GTNNAC 6 cut(s) 37, 58, 229, 569, 1044, 1171
HpyCH4III ACNGT 1 cut(s) 1094
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 5 cut(s) 480, 523, 601, 891, 1188
Hsp92II CATG 7 cut(s) 32, 64, 349, 544, 598, 980, 1164
Kzo9I GATC 4 cut(s) 273, 511, 597, 716
LmnI GCTCC 2 cut(s) 418, 492
Lsp1109I GCAGC 1 cut(s) 386
LweI GCATC 4 cut(s) 467, 775, 1053, 1163
MaeI CTAG 8 cut(s) 9, 204, 279, 503, 641, 1056, 1101, 1124
MaeIII GTNAC 1 cut(s) 63
MalI GATC 4 cut(s) 275, 513, 599, 718
MboI GATC 4 cut(s) 273, 511, 597, 716
MboII GAAGA 6 cut(s) 206, 209, 419, 521, 569, 824
MflI RGATCY 1 cut(s) 716
MhlI GDGCHC 2 cut(s) 60, 423
MlsI TGGCCA 1 cut(s) 436
MluCI AATT 5 cut(s) 290, 587, 849, 930, 1004
MluNI TGGCCA 1 cut(s) 436
MlyI GAGTC 5 cut(s) 180, 316, 555, 836, 851
Mox20I TGGCCA 1 cut(s) 436
MscI TGGCCA 1 cut(s) 436
MseI TTAA 2 cut(s) 446, 959
MslI CAYNNNNRTG 2 cut(s) 696, 1159
Msp20I TGGCCA 1 cut(s) 436
MspI CCGG 2 cut(s) 498, 608
MspR9I CCNGG 2 cut(s) 455, 551
MvaI CCWGG 2 cut(s) 455, 551
MwoI GCNNNNNNNGC 1 cut(s) 359
NcoI CCATGG 1 cut(s) 28
NdeI CATATG 1 cut(s) 693
NdeII GATC 4 cut(s) 273, 511, 597, 716
NlaIII CATG 7 cut(s) 32, 64, 349, 544, 598, 980, 1164
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 64
PaeR7I CTCGAG 2 cut(s) 824, 863
PaqCI CACCTGC 1 cut(s) 696
PceI AGGCCT 1 cut(s) 1141
PciI ACATGT 1 cut(s) 60
PfeI GAWTC 3 cut(s) 113, 158, 637
PflMI CCANNNNNTGG 1 cut(s) 34
PinAI ACCGGT 1 cut(s) 497
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 5 cut(s) 179, 316, 554, 835, 851
PpsI GAGTC 5 cut(s) 179, 316, 554, 835, 851
PscI ACATGT 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 453, 549
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 2 cut(s) 453, 549
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 4 cut(s) 37, 473, 755, 800
PsuI RGATCY 1 cut(s) 716
RsaI GTAC 3 cut(s) 241, 1045, 1091
RsaNI GTAC 3 cut(s) 240, 1044, 1090
RseI CAYNNNNRTG 2 cut(s) 696, 1159
SaqAI TTAA 2 cut(s) 446, 959
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 4 cut(s) 273, 511, 597, 716
Sau96I GGNCC 4 cut(s) 37, 473, 755, 800
SchI GAGTC 5 cut(s) 180, 316, 555, 836, 851
ScrFI CCNGG 2 cut(s) 455, 551
SduI GDGCHC 2 cut(s) 60, 423
SfaNI GCATC 4 cut(s) 467, 775, 1053, 1163
Sfr274I CTCGAG 2 cut(s) 824, 863
SinI GGWCC 2 cut(s) 37, 755
SlaI CTCGAG 2 cut(s) 824, 863
SmiMI CAYNNNNRTG 2 cut(s) 696, 1159
SmlI CTYRAG 3 cut(s) 646, 824, 863
SmoI CTYRAG 3 cut(s) 646, 824, 863
Sse9I AATT 5 cut(s) 290, 587, 849, 930, 1004
SseBI AGGCCT 1 cut(s) 1141
SsiI CCGC 1 cut(s) 20
SspMI CTAG 8 cut(s) 9, 204, 279, 503, 641, 1056, 1101, 1124
StuI AGGCCT 1 cut(s) 1141
StyD4I CCNGG 2 cut(s) 453, 549
StyI CCWWGG 3 cut(s) 28, 40, 1123
TaaI ACNGT 1 cut(s) 1094
TaqI TCGA 2 cut(s) 825, 864
TasI AATT 5 cut(s) 290, 587, 849, 930, 1004
TatI WGTACW 1 cut(s) 1043
TfiI GAWTC 3 cut(s) 113, 158, 637
Tru1I TTAA 2 cut(s) 446, 959
Tru9I TTAA 2 cut(s) 446, 959
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 6 cut(s) 105, 334, 372, 914, 942, 993
TspGWI ACGGA 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 34
VneI GTGCAC 1 cut(s) 56
VpaK11BI GGWCC 2 cut(s) 37, 755
XapI RAATTY 2 cut(s) 587, 930
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 1 cut(s) 64
XhoI CTCGAG 2 cut(s) 824, 863
XmaJI CCTAGG 1 cut(s) 1123
XspI CTAG 8 cut(s) 9, 204, 279, 503, 641, 1056, 1101, 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.