MD05G1229500.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0017624
Physical Location & Seq
Reverse (-)
36294983 .. 36296611
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1229500.v1.1.491

Sequence Viewer

Length: 1629 bp
ATGGAAGATTTCAATGCTTGGCTCTCCTTCCCCTTGCATGAGAACCTCAACCATGATCTCGCCATTCGGAGGTTTTGTCCGGCGCGAGTTGAGCAAGAACAAGGAGGAGGTGGAGGTGGAGAGTGGGATGAAATGCTAAATGAGATTGAGTTTTCTTCATCCTCTCACACCACTAGCACTCCATGCTTGCCTCCTTCAGAGGTTGACGAATTCGTCGATAGCTTCATCAACATGGATCATTGCATTGATGATCATGACATTCAGTCCATGGAGAAACAACAAAGTAGTAGTAGTATTGAGTACTATGATCAAGATGGGATCGAGACATTTTCAATGGCAGATGATCTGTATGGAGATGTTCTGATGATGATGGAGGAAGAAGATGATCATGTGGATATGAGTGGCTTGGCGGAAGATTTTCGGGTTGCGAAAACTGAGATGATGCCTGGGGTTGAGGTGGCGAGCCATGGTGTGGACCAAGGGCTTAACTTGGTGCACATGTTGCTCGCTTGTGCCGAGGCTGTTGGCTGCAGGGACACCAAGCTTGCAGACTCATTGCTCGCCCAAATTTGGGCATCTGTGAGCCCTTGGGGTGACTCCTTGCAAAGAGTTTCATACTGCTTTGCAATGGGATTGAAATCAAGGCTTTCGCTTCTTCAAAATGTCAACATAAATGGCACATTCAACAACGATATGATGGATGTTTCAGTGACCTCTAGGGACGACAAGTTGGAAGCTTTTCAACTCCTTAACCAAACAACTCCTTACATCGCTTTCGGTTTCATGGTTGCCAATGAAGCTATATGCCAAGCAGCGCAAGGGAAGGAAGACTTGCACATCATTGATTTGGGAATGGAGCACACTCTTCAGTGGCCTTCTTTGATTAGAACCCTAGCATCGAGACCCGAAGGCCCGCCTAAGAAGCTGCGAATCACCGGACTCGTTGAAGATCACAATCTGTTTGAGCTTGAAGCTGGCATGAGAGCTCTGGTGGAGGAGGCTAGCTCGCTAGGGATACCAACAGAATTTAGTTTGATATCGGAGCCGGTGAAACCATCACTCCTAACAAGAGAGAACCTAGACTTGAGAGAAGGGGAAGCATTATTTGTCAATAGCATCATGCACTTGCATAAGTATGTCAAAGAGAGTAGAGGCTCTCTGAAGGCAATTCTTCAAGCTCTGAAGAAATTAGGTCCATCTGTGCTTACATTGGTAGAACAAGATGCAAACCACAACGGGCCATTTTTCCTCGGGAGATTCCTCGAGTCTCTTCACTTTTACTCAGCAATTTTCGACTCCCTTGAGGCTAGCCTCCCACGAAACAGCCCTCAGAGGATGAAGATCGAGAGGCAGCACTTCGCGGACGAAATCAGAAACATTGTTGCTTTTGAGGGATTAAATAGGATTGAGAGGCATGAGAGGGCAGACCAGTGGAGAAGGCAATTAGGGCGAGCCGGGTTTCAAGTGATGGGGTTGAAGTGTACAAGTCAAGCAAGGATGATGCTATCTGTTTATGGCTGTGATGGCTACACTTTGGCTAGTGAGAAGGGTTGTCTCCTCCTCGGGTGGAAAGGCAGGCCGATAATGCTGGCATCGGCATGGCAAGCGCACAGCGTGTCTTCCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

543

Amino Acids

60.68

Weight (kDa)

4.86

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 164 - 535 8.7e-108 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017624)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g14680
malus_domestica MD05G1229500.v1.1
prunus_persica Prupe.4G132100_v2.0.a1
pyrus_communis pycom05g21040
rosa_chinensis RchiOBHm_Chr5g0024171
rosa_laevigata RLG00000032776
rosa_multiflora Rmu_sc0004033.1_g000010
rosa_rugosa Rorug05G0082200
rosa_samantha Rh5BG169400 Rh5CG185300 Rh5DG171700
rosa_wichuraiana Rw5G015590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccB7I CCANNNNNTGG 1 cut(s) 472
AccII CGCG 2 cut(s) 85, 1361
AciI CCGC 3 cut(s) 410, 914, 1361
AclWI GGATC 2 cut(s) 243, 326
AcsI RAATTY 3 cut(s) 209, 567, 1025
AcuI CTGAAG 4 cut(s) 180, 851, 1181, 1202
AdeI CACNNNGTG 1 cut(s) 1615
AfaI GTAC 2 cut(s) 302, 1483
AfiI CCNNNNNNNGG 4 cut(s) 69, 472, 570, 571
AflIII ACRYGT 1 cut(s) 498
AhdI GACNNNNNGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 445
Alw21I GWGCWC 3 cut(s) 498, 861, 988
Alw26I GTCTC 4 cut(s) 317, 895, 1272, 1559
Alw44I GTGCAC 1 cut(s) 494
AlwI GGATC 2 cut(s) 243, 326
Ama87I CYCGRG 3 cut(s) 1250, 1262, 1562
AoxI GGCC 4 cut(s) 872, 910, 1238, 1577
ApaLI GTGCAC 1 cut(s) 494
ApeKI GCWGC 4 cut(s) 528, 812, 925, 1351
ApoI RAATTY 3 cut(s) 209, 567, 1025
Asp700I GAANNNNTTC 2 cut(s) 417, 738
AspLEI GCGC 3 cut(s) 85, 817, 1609
AspS9I GGNCC 4 cut(s) 475, 911, 1193, 1238
AsuC2I CCSGG 1 cut(s) 1456
AsuHPI GGTGA 3 cut(s) 605, 925, 1060
AsuNHI GCTAGC 2 cut(s) 1001, 1307
AvaI CYCGRG 3 cut(s) 1250, 1262, 1562
AvaII GGWCC 2 cut(s) 475, 1193
BaeGI GKGCMC 1 cut(s) 498
BanII GRGCYC 2 cut(s) 587, 988
BbsI GAAGAC 2 cut(s) 834, 1611
Bbv12I GWGCWC 3 cut(s) 498, 861, 988
BbvI GCAGC 4 cut(s) 515, 824, 912, 1363
BccI CCATC 7 cut(s) 308, 364, 691, 1063, 1204, 1462, 1517
BciT130I CCWGG 1 cut(s) 447
BciVI GTATCC 1 cut(s) 1008
BclI TGATCA 3 cut(s) 250, 307, 385
BcnI CCSGG 1 cut(s) 1456
BcoDI GTCTC 4 cut(s) 317, 895, 1272, 1559
BfaI CTAG 9 cut(s) 174, 717, 893, 1002, 1010, 1079, 1308, 1539, 1627
BfmI CTRYAG 1 cut(s) 529
BfuI GTATCC 1 cut(s) 1008
BisI GCNGC 4 cut(s) 529, 813, 926, 1352
BlsI GCNGC 4 cut(s) 530, 814, 927, 1353
BmcAI AGTACT 1 cut(s) 302
Bme1390I CCNGG 2 cut(s) 447, 1456
Bme18I GGWCC 2 cut(s) 475, 1193
BmeRI GACNNNNNGTC 1 cut(s) 262
BmeT110I CYCGRG 3 cut(s) 1250, 1262, 1562
BmgT120I GGNCC 4 cut(s) 475, 911, 1193, 1238
BmiI GGNNCC 1 cut(s) 1044
BmrFI CCNGG 2 cut(s) 447, 1456
BmsI GCATC 7 cut(s) 432, 584, 905, 1125, 1213, 1491, 1601
BmtI GCTAGC 2 cut(s) 1005, 1311
BpiI GAAGAC 2 cut(s) 834, 1611
BplI GAGNNNNNCTC 2 cut(s) 989, 1021
BpuEI CTTGAG 2 cut(s) 1105, 1322
BpuMI CCSGG 1 cut(s) 1456
BsaBI GATNNNNATC 3 cut(s) 637, 954, 1340
BsaI GGTCTC 1 cut(s) 895
BsaJI CCNNGG 8 cut(s) 267, 446, 466, 478, 516, 587, 1249, 1561
BsaWI WCCGGW 1 cut(s) 935
BsaXI ACNNNNNCTCC 6 cut(s) 61, 91, 163, 193, 1044, 1074
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 472, 570, 571
Bse118I RCCGGY 1 cut(s) 1045
Bse1I ACTGG 1 cut(s) 1429
Bse3DI GCAATG 3 cut(s) 238, 554, 633
Bse8I GATNNNNATC 3 cut(s) 637, 954, 1340
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 8 cut(s) 267, 446, 466, 478, 516, 587, 1249, 1561
BseGI GGATG 5 cut(s) 133, 158, 706, 1341, 1503
BseJI GATNNNNATC 3 cut(s) 637, 954, 1340
BseLI CCNNNNNNNGG 4 cut(s) 69, 472, 570, 571
BseMI GCAATG 3 cut(s) 238, 554, 633
BseMII CTCAG 3 cut(s) 426, 1296, 1343
BseNI ACTGG 1 cut(s) 1429
BseRI GAGGAG 4 cut(s) 120, 1010, 1547, 1550
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 4 cut(s) 515, 824, 912, 1363
Bsh1236I CGCG 2 cut(s) 85, 1361
BshFI GGCC 4 cut(s) 874, 912, 1240, 1579
BsiHKAI GWGCWC 3 cut(s) 498, 861, 988
BsiHKCI CYCGRG 3 cut(s) 1250, 1262, 1562
BsiSI CCGG 4 cut(s) 80, 936, 1046, 1455
BslFI GGGAC 2 cut(s) 548, 734
BslI CCNNNNNNNGG 4 cut(s) 69, 472, 570, 571
BsmAI GTCTC 4 cut(s) 317, 895, 1272, 1559
BsmFI GGGAC 2 cut(s) 548, 734
BsnI GGCC 4 cut(s) 874, 912, 1240, 1579
Bso31I GGTCTC 1 cut(s) 895
BsoBI CYCGRG 3 cut(s) 1250, 1262, 1562
Bsp1286I GDGCHC 4 cut(s) 498, 587, 861, 988
Bsp1407I TGTACA 1 cut(s) 1481
Bsp143I GATC 9 cut(s) 55, 235, 250, 307, 318, 343, 385, 949, 1341
Bsp19I CCATGG 2 cut(s) 267, 466
BspACI CCGC 3 cut(s) 410, 914, 1361
BspANI GGCC 4 cut(s) 874, 912, 1240, 1579
BspCNI CTCAG 3 cut(s) 427, 1295, 1342
BspFNI CGCG 2 cut(s) 85, 1361
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 1044
BspMAI CTGCAG 1 cut(s) 533
BspOI GCTAGC 2 cut(s) 1005, 1311
BspPI GGATC 2 cut(s) 243, 326
BspTNI GGTCTC 1 cut(s) 895
BsrDI GCAATG 3 cut(s) 238, 554, 633
BsrFI RCCGGY 1 cut(s) 1045
BsrGI TGTACA 1 cut(s) 1481
BsrI ACTGG 1 cut(s) 1429
BssAI RCCGGY 1 cut(s) 1045
BssECI CCNNGG 8 cut(s) 267, 446, 466, 478, 516, 587, 1249, 1561
BssMI GATC 9 cut(s) 55, 235, 250, 307, 318, 343, 385, 949, 1341
BssT1I CCWWGG 4 cut(s) 267, 466, 478, 587
Bst2UI CCWGG 1 cut(s) 447
Bst6I CTCTTC 2 cut(s) 870, 1275
BstAPI GCANNNNNTGC 2 cut(s) 183, 502
BstAUI TGTACA 1 cut(s) 1481
BstDEI CTNAG 4 cut(s) 435, 918, 1282, 1329
BstDSI CCRYGG 2 cut(s) 267, 466
BstF5I GGATG 5 cut(s) 133, 158, 706, 1341, 1503
BstFNI CGCG 2 cut(s) 85, 1361
BstHHI GCGC 3 cut(s) 85, 817, 1609
BstKTI GATC 9 cut(s) 58, 238, 253, 310, 321, 346, 388, 952, 1344
BstMAI GTCTC 4 cut(s) 317, 895, 1272, 1559
BstMBI GATC 9 cut(s) 55, 235, 250, 307, 318, 343, 385, 949, 1341
BstMWI GCNNNNNNNGC 9 cut(s) 91, 183, 502, 797, 922, 1447, 1524, 1585, 1604
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 2 cut(s) 445, 1454
BstSFI CTRYAG 1 cut(s) 529
BstSLI GKGCMC 1 cut(s) 498
BstUI CGCG 2 cut(s) 85, 1361
BstV1I GCAGC 4 cut(s) 515, 824, 912, 1363
BstV2I GAAGAC 2 cut(s) 834, 1611
BsuI GTATCC 1 cut(s) 1008
BsuRI GGCC 4 cut(s) 874, 912, 1240, 1579
BtgI CCRYGG 2 cut(s) 267, 466
BtgZI GCGATG 1 cut(s) 754
BtsCI GGATG 5 cut(s) 133, 158, 706, 1341, 1503
BtsIMutI CAGTG 3 cut(s) 714, 875, 1436
CciI TCATGA 1 cut(s) 253
CfoI GCGC 3 cut(s) 85, 817, 1609
Cfr10I RCCGGY 1 cut(s) 1045
Cfr13I GGNCC 4 cut(s) 475, 911, 1193, 1238
Csp6I GTAC 2 cut(s) 301, 1482
CviQI GTAC 2 cut(s) 301, 1482
DdeI CTNAG 4 cut(s) 435, 918, 1282, 1329
DpnI GATC 9 cut(s) 57, 237, 252, 309, 320, 345, 387, 951, 1343
DpnII GATC 9 cut(s) 55, 235, 250, 307, 318, 343, 385, 949, 1341
DraIII CACNNNGTG 1 cut(s) 1615
DrdI GACNNNNNNGTC 1 cut(s) 212
DriI GACNNNNNGTC 1 cut(s) 262
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 2 cut(s) 870, 1275
Eam1105I GACNNNNNGTC 1 cut(s) 262
EarI CTCTTC 2 cut(s) 870, 1275
EciI GGCGGA 1 cut(s) 425
Ecl136II GAGCTC 1 cut(s) 986
Eco130I CCWWGG 4 cut(s) 267, 466, 478, 587
Eco24I GRGCYC 2 cut(s) 587, 988
Eco31I GGTCTC 1 cut(s) 895
Eco32I GATATC 1 cut(s) 1038
Eco47I GGWCC 2 cut(s) 475, 1193
Eco53kI GAGCTC 1 cut(s) 986
Eco57I CTGAAG 4 cut(s) 180, 851, 1181, 1202
Eco88I CYCGRG 3 cut(s) 1250, 1262, 1562
EcoICRI GAGCTC 1 cut(s) 986
EcoRI GAATTC 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 445
EcoRV GATATC 1 cut(s) 1038
EcoT14I CCWWGG 4 cut(s) 267, 466, 478, 587
EcoT38I GRGCYC 2 cut(s) 587, 988
ErhI CCWWGG 4 cut(s) 267, 466, 478, 587
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 2 cut(s) 548, 734
FauI CCCGC 1 cut(s) 921
FbaI TGATCA 3 cut(s) 250, 307, 385
Fnu4HI GCNGC 4 cut(s) 529, 813, 926, 1352
FokI GGATG 5 cut(s) 140, 145, 713, 1348, 1510
FriOI GRGCYC 2 cut(s) 587, 988
Fsp4HI GCNGC 4 cut(s) 529, 813, 926, 1352
FspBI CTAG 9 cut(s) 174, 717, 893, 1002, 1010, 1079, 1308, 1539, 1627
GlaI GCGC 3 cut(s) 84, 816, 1608
GluI GCNGC 4 cut(s) 529, 813, 926, 1352
HaeIII GGCC 4 cut(s) 874, 912, 1240, 1579
HapII CCGG 4 cut(s) 80, 936, 1046, 1455
HhaI GCGC 3 cut(s) 85, 817, 1609
Hin6I GCGC 3 cut(s) 83, 815, 1607
HinP1I GCGC 3 cut(s) 83, 815, 1607
HincII GTYRAC 2 cut(s) 205, 667
HindII GTYRAC 2 cut(s) 205, 667
HindIII AAGCTT 2 cut(s) 542, 735
HinfI GANTC 7 cut(s) 551, 596, 930, 939, 1257, 1265, 1295
HpaII CCGG 4 cut(s) 80, 936, 1046, 1455
HphI GGTGA 3 cut(s) 605, 925, 1060
Hpy166II GTNNAC 5 cut(s) 205, 475, 496, 667, 1482
Hpy188I TCNGA 8 cut(s) 69, 199, 363, 1042, 1161, 1182, 1332, 1373
Hpy188III TCNNGA 6 cut(s) 254, 311, 322, 900, 1252, 1345
Hpy8I GTNNAC 5 cut(s) 205, 475, 496, 667, 1482
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 9 cut(s) 37, 204, 817, 885, 902, 1085, 1156, 1431, 1540
HpyF10VI GCNNNNNNNGC 9 cut(s) 91, 183, 502, 797, 922, 1447, 1524, 1585, 1604
HpyF3I CTNAG 4 cut(s) 435, 918, 1282, 1329
HspAI GCGC 3 cut(s) 83, 815, 1607
Ksp22I TGATCA 3 cut(s) 250, 307, 385
Kzo9I GATC 9 cut(s) 55, 235, 250, 307, 318, 343, 385, 949, 1341
LmnI GCTCC 2 cut(s) 856, 1042
Lsp1109I GCAGC 4 cut(s) 515, 824, 912, 1363
LweI GCATC 7 cut(s) 432, 584, 905, 1125, 1213, 1491, 1601
MaeI CTAG 9 cut(s) 174, 717, 893, 1002, 1010, 1079, 1308, 1539, 1627
MaeIII GTNAC 2 cut(s) 593, 709
MalI GATC 9 cut(s) 57, 237, 252, 309, 320, 345, 387, 951, 1343
MboI GATC 9 cut(s) 55, 235, 250, 307, 318, 343, 385, 949, 1341
MhlI GDGCHC 4 cut(s) 498, 587, 861, 988
MluCI AATT 7 cut(s) 209, 567, 1025, 1167, 1187, 1287, 1442
MlyI GAGTC 5 cut(s) 545, 590, 933, 1274, 1289
MmeI TCCRAC 1 cut(s) 711
MroXI GAANNNNTTC 2 cut(s) 417, 738
MseI TTAA 3 cut(s) 486, 750, 1397
MslI CAYNNNNRTG 3 cut(s) 230, 1134, 1597
MspI CCGG 4 cut(s) 80, 936, 1046, 1455
MspR9I CCNGG 2 cut(s) 447, 1456
MvaI CCWGG 1 cut(s) 447
MvnI CGCG 2 cut(s) 85, 1361
MwoI GCNNNNNNNGC 9 cut(s) 91, 183, 502, 797, 922, 1447, 1524, 1585, 1604
NciI CCSGG 1 cut(s) 1456
NcoI CCATGG 2 cut(s) 267, 466
NdeII GATC 9 cut(s) 55, 235, 250, 307, 318, 343, 385, 949, 1341
NheI GCTAGC 2 cut(s) 1001, 1307
NlaIV GGNNCC 1 cut(s) 1044
NmeAIII GCCGAG 1 cut(s) 541
NmuCI GTSAC 2 cut(s) 593, 709
NspI RCATGY 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 1262
PagI TCATGA 1 cut(s) 253
PciI ACATGT 1 cut(s) 498
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 513
PdmI GAANNNNTTC 2 cut(s) 417, 738
PfeI GAWTC 2 cut(s) 930, 1257
PflMI CCANNNNNTGG 1 cut(s) 472
PkrI GCNGC 4 cut(s) 530, 814, 927, 1353
PleI GAGTC 5 cut(s) 545, 590, 933, 1273, 1289
PpsI GAGTC 5 cut(s) 545, 590, 933, 1273, 1289
PscI ACATGT 1 cut(s) 498
Psp124BI GAGCTC 1 cut(s) 988
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 1044
PspPI GGNCC 4 cut(s) 475, 911, 1193, 1238
PspXI VCTCGAGB 1 cut(s) 1262
PstI CTGCAG 1 cut(s) 533
RsaI GTAC 2 cut(s) 302, 1483
RsaNI GTAC 2 cut(s) 301, 1482
RseI CAYNNNNRTG 3 cut(s) 230, 1134, 1597
SacI GAGCTC 1 cut(s) 988
SaqAI TTAA 3 cut(s) 486, 750, 1397
SatI GCNGC 4 cut(s) 529, 813, 926, 1352
Sau3AI GATC 9 cut(s) 55, 235, 250, 307, 318, 343, 385, 949, 1341
Sau96I GGNCC 4 cut(s) 475, 911, 1193, 1238
ScaI AGTACT 1 cut(s) 302
SchI GAGTC 5 cut(s) 545, 590, 933, 1274, 1289
ScrFI CCNGG 2 cut(s) 447, 1456
SduI GDGCHC 4 cut(s) 498, 587, 861, 988
SfaNI GCATC 7 cut(s) 432, 584, 905, 1125, 1213, 1491, 1601
SfcI CTRYAG 1 cut(s) 529
Sfr274I CTCGAG 1 cut(s) 1262
SinI GGWCC 2 cut(s) 475, 1193
SlaI CTCGAG 1 cut(s) 1262
SmiMI CAYNNNNRTG 3 cut(s) 230, 1134, 1597
SmlI CTYRAG 3 cut(s) 1084, 1262, 1301
SmoI CTYRAG 3 cut(s) 1084, 1262, 1301
Sse9I AATT 7 cut(s) 209, 567, 1025, 1167, 1187, 1287, 1442
SsiI CCGC 3 cut(s) 410, 914, 1361
SspMI CTAG 9 cut(s) 174, 717, 893, 1002, 1010, 1079, 1308, 1539, 1627
SstI GAGCTC 1 cut(s) 988
StyD4I CCNGG 2 cut(s) 445, 1454
StyI CCWWGG 4 cut(s) 267, 466, 478, 587
TaqI TCGA 6 cut(s) 216, 321, 899, 1263, 1293, 1344
TasI AATT 7 cut(s) 209, 567, 1025, 1167, 1187, 1287, 1442
TatI WGTACW 2 cut(s) 300, 1481
TfiI GAWTC 2 cut(s) 930, 1257
Tru1I TTAA 3 cut(s) 486, 750, 1397
Tru9I TTAA 3 cut(s) 486, 750, 1397
TscAI CASTG 3 cut(s) 714, 875, 1436
TseFI GTSAC 2 cut(s) 593, 709
TseI GCWGC 4 cut(s) 528, 812, 925, 1351
Tsp45I GTSAC 2 cut(s) 593, 709
TspDTI ATGAA 7 cut(s) 144, 147, 214, 603, 772, 810, 1352
TspRI CASTG 3 cut(s) 714, 875, 1436
Van91I CCANNNNNTGG 1 cut(s) 472
VneI GTGCAC 1 cut(s) 494
VpaK11BI GGWCC 2 cut(s) 475, 1193
XapI RAATTY 3 cut(s) 209, 567, 1025
XceI RCATGY 1 cut(s) 502
XhoI CTCGAG 1 cut(s) 1262
XmnI GAANNNNTTC 2 cut(s) 417, 738
XspI CTAG 9 cut(s) 174, 717, 893, 1002, 1010, 1079, 1308, 1539, 1627
ZrmI AGTACT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.