FvH4_3g19280

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010659
Physical Location & Seq
Reverse (-)
12460515 .. 12467237
6723 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19280.t2

Sequence Viewer

Length: 459 bp
ATGTCGTCGACGACCACCAACAAAGCGGGAACGGCGTCAGCCCAGCAGAGCAACGCGAAGCCAGGGCTCCGAAAGCCCGTGTTCGTCAAGGTGAGCGATCTCAAGCCCGGAACGAGCGGCCACACTCTCGTCGTCAAGGTCATCAACGCCACCACGGTGTTGCAGAAGGGCCGATCGGTGTCGCAGCATCTCCGCCAGAGCCGAATCTCCGAGTGCCTCATTGGGGATGAGACCGGGACCATTCTCTTCACTGCTAGAAATGAGCAAGTTGATTTGATGAAGCCTGACTCCACTGTTATTATCCGCAATGCTAAGATCGACATGTTTAAGGGATCCATGAGGCTAGCAGTTGACAAATGGGGCCGTATTGAGGTGACTGAGCCTGCAAAGTTTGTAGTAAAGGATAACAACAATCTCTCTTTAGTTGAATATGAGCTGGTTAGTGTTGTGGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.64

Weight (kDa)

9.48

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 34 - 125 8.4e-46 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 117
AccI GTMKAC 1 cut(s) 8
AccII CGCG 1 cut(s) 56
AciI CCGC 4 cut(s) 26, 117, 193, 304
AclWI GGATC 2 cut(s) 327, 340
AcoI YGGCCR 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 35
AfiI CCNNNNNNNGG 2 cut(s) 223, 370
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 1 cut(s) 428
AjnI CCWGG 1 cut(s) 61
AleI CACNNNNGTG 1 cut(s) 155
AluBI AGCT 1 cut(s) 436
AluI AGCT 1 cut(s) 436
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 2 cut(s) 327, 340
AoxI GGCC 3 cut(s) 118, 169, 361
ApeKI GCWGC 1 cut(s) 184
AspS9I GGNCC 3 cut(s) 169, 237, 361
AsuC2I CCSGG 2 cut(s) 108, 235
AsuHPI GGTGA 2 cut(s) 103, 385
AsuNHI GCTAGC 1 cut(s) 343
AvaII GGWCC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 196
BceAI ACGGC 2 cut(s) 48, 348
BciT130I CCWGG 1 cut(s) 63
BcnI CCSGG 2 cut(s) 108, 235
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 2 cut(s) 255, 344
BisI GCNGC 2 cut(s) 118, 185
BlsI GCNGC 2 cut(s) 119, 186
Bme1390I CCNGG 3 cut(s) 63, 108, 235
Bme18I GGWCC 1 cut(s) 237
BmgT120I GGNCC 3 cut(s) 169, 237, 361
BmiI GGNNCC 4 cut(s) 68, 238, 334, 362
BmrFI CCNGG 3 cut(s) 63, 108, 235
BmsI GCATC 1 cut(s) 196
BmtI GCTAGC 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 2 cut(s) 108, 235
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 62, 153
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 370
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 62, 153
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 2 cut(s) 223, 370
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 369
BseXI GCAGC 1 cut(s) 196
BseYI CCCAGC 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 56
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 3 cut(s) 120, 171, 363
BsiEI CGRYCG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 108, 234
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 223, 370
BsmAI GTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 3 cut(s) 120, 171, 363
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 4 cut(s) 97, 173, 315, 332
BspACI CCGC 4 cut(s) 26, 117, 193, 304
BspANI GGCC 3 cut(s) 120, 171, 363
BspCNI CTCAG 1 cut(s) 370
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 4 cut(s) 68, 238, 334, 362
BspOI GCTAGC 1 cut(s) 347
BspPI GGATC 2 cut(s) 327, 340
BspTNI GGTCTC 1 cut(s) 224
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 62, 153
BssMI GATC 4 cut(s) 97, 173, 315, 332
BssNI GRCGYC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 2 cut(s) 157, 295
Bst6I CTCTTC 1 cut(s) 251
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 345, 384
BstDEI CTNAG 2 cut(s) 312, 378
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 232
BstFNI CGCG 1 cut(s) 56
BstKTI GATC 4 cut(s) 100, 176, 318, 335
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 4 cut(s) 97, 173, 315, 332
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 2 cut(s) 32, 73
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 1 cut(s) 325
BstSCI CCNGG 3 cut(s) 61, 106, 233
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 196
BstX2I RGATCY 1 cut(s) 332
BstYI RGATCY 1 cut(s) 332
BsuRI GGCC 3 cut(s) 120, 171, 363
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 249, 291
Cac8I GCNNGC 2 cut(s) 345, 384
Cfr13I GGNCC 3 cut(s) 169, 237, 361
CseI GACGC 1 cut(s) 24
CviAII CATG 2 cut(s) 322, 337
DdeI CTNAG 2 cut(s) 312, 378
DpnI GATC 4 cut(s) 99, 175, 317, 334
DpnII GATC 4 cut(s) 97, 173, 315, 332
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 69
FaeI CATG 2 cut(s) 325, 340
FaiI YATR 3 cut(s) 323, 338, 432
FaqI GGGAC 1 cut(s) 250
FatI CATG 2 cut(s) 321, 336
FauI CCCGC 1 cut(s) 19
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 2 cut(s) 118, 185
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 2 cut(s) 118, 185
FspBI CTAG 2 cut(s) 255, 344
GluI GCNGC 2 cut(s) 118, 185
GsaI CCCAGC 1 cut(s) 46
HaeIII GGCC 3 cut(s) 120, 171, 363
HapII CCGG 2 cut(s) 108, 234
HgaI GACGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 2 cut(s) 325, 340
HincII GTYRAC 2 cut(s) 9, 352
HindII GTYRAC 2 cut(s) 9, 352
HinfI GANTC 2 cut(s) 204, 287
HpaII CCGG 2 cut(s) 108, 234
HphI GGTGA 2 cut(s) 103, 385
Hpy166II GTNNAC 2 cut(s) 9, 352
Hpy188I TCNGA 2 cut(s) 71, 211
Hpy8I GTNNAC 2 cut(s) 9, 352
Hpy99I CGWCG 3 cut(s) 10, 13, 134
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 2 cut(s) 157, 295
HpyCH4V TGCA 2 cut(s) 163, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 73
HpyF3I CTNAG 2 cut(s) 312, 378
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 2 cut(s) 325, 340
Kzo9I GATC 4 cut(s) 97, 173, 315, 332
LmnI GCTCC 1 cut(s) 72
LpnPI CCDG 9 cut(s) 48, 56, 75, 121, 209, 247, 297, 396, 422
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 1 cut(s) 196
MaeI CTAG 2 cut(s) 255, 344
MaeIII GTNAC 1 cut(s) 373
MalI GATC 4 cut(s) 99, 175, 317, 334
MbiI CCGCTC 1 cut(s) 117
MboI GATC 4 cut(s) 97, 173, 315, 332
MboII GAAGA 1 cut(s) 238
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 1 cut(s) 69
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 4 cut(s) 227, 333, 364, 445
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 2 cut(s) 108, 234
MspR9I CCNGG 3 cut(s) 63, 108, 235
MvaI CCWGG 1 cut(s) 63
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 2 cut(s) 32, 73
NciI CCSGG 2 cut(s) 108, 235
NdeII GATC 4 cut(s) 97, 173, 315, 332
NheI GCTAGC 1 cut(s) 343
NlaIII CATG 2 cut(s) 325, 340
NlaIV GGNNCC 4 cut(s) 68, 238, 334, 362
NmuCI GTSAC 1 cut(s) 373
NspI RCATGY 1 cut(s) 325
OliI CACNNNNGTG 1 cut(s) 155
PciI ACATGT 1 cut(s) 321
PfeI GAWTC 1 cut(s) 204
PkrI GCNGC 2 cut(s) 119, 186
Ple19I CGATCG 1 cut(s) 176
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PscI ACATGT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 4 cut(s) 68, 238, 334, 362
PspPI GGNCC 3 cut(s) 169, 237, 361
PsuI RGATCY 1 cut(s) 332
PvuI CGATCG 1 cut(s) 176
RseI CAYNNNNRTG 1 cut(s) 155
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 2 cut(s) 118, 185
Sau3AI GATC 4 cut(s) 97, 173, 315, 332
Sau96I GGNCC 3 cut(s) 169, 237, 361
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 3 cut(s) 63, 108, 235
SduI GDGCHC 1 cut(s) 69
SetI ASST 4 cut(s) 93, 141, 375, 438
SfaNI GCATC 1 cut(s) 196
SgrDI CGTCGACG 1 cut(s) 7
SinI GGWCC 1 cut(s) 237
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SsiI CCGC 4 cut(s) 26, 117, 193, 304
SspMI CTAG 2 cut(s) 255, 344
StyD4I CCNGG 3 cut(s) 61, 106, 233
TaaI ACNGT 2 cut(s) 157, 295
TaqI TCGA 2 cut(s) 8, 318
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 2 cut(s) 256, 298
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 1 cut(s) 293
TspRI CASTG 2 cut(s) 256, 298
VpaK11BI GGWCC 1 cut(s) 237
XceI RCATGY 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 2 cut(s) 255, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.