Rh5BG225700

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010659
Physical Location & Seq
Reverse (-)
26217918 .. 26220097
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG225700.1

Sequence Viewer

Length: 462 bp
ATGTCGTCGACGACCACCACCAACAAAGCGGGACCGGCGTCGGCCCAGCAGAGCAATGCGAAGCCGGGGCTCCGAAAGCCCGTGTTCGTCAAGGTCAGCGATCTCAGGCCCGGGACGAGCGGCCACACTCTCGTCGTCAAGGTCATCAGCTCCACCACCGTGTTGCAGAAGGGCCGATCGGTGTCTCAGCACCTCCGCCAGACCCGAATCTCCGAGTGCCTCATTGGGGATGAGACCGGGACCATTCTCTTCACCGCCAGAAATGATCAAGTTGACTTGATGAAACCTGATACCACTGTTATTCTCCGGAATGCTAAGATCGACATGTTTAAGGGATCCATGAGGCTAGCAGTTGACAAATGGGGCCGTATTGAGGTGACCGAGCCTGCAAACTTTGTAGTCAAGGAGAACAACAATCTCTCTTTAGTTGAATATGAGCTGGTGAGTGTTGTAGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.77

Weight (kDa)

9.37

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 35 - 126 7.4e-46 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 120
AccI GTMKAC 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 306
AciI CCGC 4 cut(s) 29, 120, 196, 255
AclWI GGATC 2 cut(s) 330, 343
AcoI YGGCCR 1 cut(s) 121
AcyI GRCGYC 1 cut(s) 38
AfiI CCNNNNNNNGG 2 cut(s) 226, 373
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 1 cut(s) 431
AleI CACNNNNGTG 1 cut(s) 158
AluBI AGCT 2 cut(s) 150, 439
AluI AGCT 2 cut(s) 150, 439
Alw26I GTCTC 2 cut(s) 189, 227
AlwI GGATC 2 cut(s) 330, 343
Ama87I CYCGRG 1 cut(s) 110
Aor13HI TCCGGA 1 cut(s) 306
AoxI GGCC 5 cut(s) 42, 107, 121, 172, 364
AspS9I GGNCC 6 cut(s) 32, 43, 108, 172, 240, 364
AsuC2I CCSGG 4 cut(s) 66, 111, 112, 238
AsuHPI GGTGA 3 cut(s) 244, 388, 454
AsuNHI GCTAGC 1 cut(s) 346
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 2 cut(s) 32, 240
BaeI ACNNNNGTAYC 2 cut(s) 282, 315
BamHI GGATCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 72
BceAI ACGGC 1 cut(s) 351
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 4 cut(s) 66, 111, 112, 238
BcoDI GTCTC 2 cut(s) 189, 227
BfaI CTAG 1 cut(s) 347
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 4 cut(s) 66, 111, 112, 238
Bme18I GGWCC 2 cut(s) 32, 240
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 6 cut(s) 32, 43, 108, 172, 240, 364
BmiI GGNNCC 5 cut(s) 33, 71, 241, 337, 365
BmrFI CCNGG 4 cut(s) 66, 111, 112, 238
BmtI GCTAGC 1 cut(s) 350
BoxI GACNNNNGTC 1 cut(s) 37
BpuMI CCSGG 4 cut(s) 66, 111, 112, 238
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 65, 110
BsaWI WCCGGW 1 cut(s) 306
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 373
Bse118I RCCGGY 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 61
BseAI TCCGGA 1 cut(s) 306
BseDI CCNNGG 2 cut(s) 65, 110
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 2 cut(s) 226, 373
BseMI GCAATG 1 cut(s) 61
BseMII CTCAG 2 cut(s) 118, 200
BseYI CCCAGC 1 cut(s) 45
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 5 cut(s) 44, 109, 123, 174, 366
BsiEI CGRYCG 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 5 cut(s) 35, 65, 111, 237, 307
BslFI GGGAC 3 cut(s) 45, 127, 253
BslI CCNNNNNNNGG 2 cut(s) 226, 373
BsmAI GTCTC 2 cut(s) 189, 227
BsmFI GGGAC 3 cut(s) 45, 127, 253
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 5 cut(s) 44, 109, 123, 174, 366
Bso31I GGTCTC 1 cut(s) 227
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 72
Bsp13I TCCGGA 1 cut(s) 306
Bsp143I GATC 5 cut(s) 100, 176, 265, 318, 335
BspACI CCGC 4 cut(s) 29, 120, 196, 255
BspANI GGCC 5 cut(s) 44, 109, 123, 174, 366
BspCNI CTCAG 2 cut(s) 117, 199
BspEI TCCGGA 1 cut(s) 306
BspLI GGNNCC 5 cut(s) 33, 71, 241, 337, 365
BspOI GCTAGC 1 cut(s) 350
BspPI GGATC 2 cut(s) 330, 343
BspTNI GGTCTC 1 cut(s) 227
BsrBI CCGCTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 65, 110
BssMI GATC 5 cut(s) 100, 176, 265, 318, 335
BssNI GRCGYC 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 160, 298
Bst6I CTCTTC 1 cut(s) 254
BstACI GRCGYC 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 348, 387
BstDEI CTNAG 3 cut(s) 104, 186, 315
BstEII GGTNACC 1 cut(s) 376
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 5 cut(s) 103, 179, 268, 321, 338
BstMAI GTCTC 2 cut(s) 189, 227
BstMBI GATC 5 cut(s) 100, 176, 265, 318, 335
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 2 cut(s) 35, 76
BstNSI RCATGY 1 cut(s) 328
BstPAI GACNNNNGTC 1 cut(s) 37
BstPI GGTNACC 1 cut(s) 376
BstSCI CCNGG 4 cut(s) 64, 109, 110, 236
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuRI GGCC 5 cut(s) 44, 109, 123, 174, 366
BtsCI GGATG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 348, 387
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 6 cut(s) 32, 43, 108, 172, 240, 364
Cfr9I CCCGGG 1 cut(s) 110
CseI GACGC 1 cut(s) 27
CviAII CATG 2 cut(s) 325, 340
DdeI CTNAG 3 cut(s) 104, 186, 315
DpnI GATC 5 cut(s) 102, 178, 267, 320, 337
DpnII GATC 5 cut(s) 100, 176, 265, 318, 335
EaeI YGGCCR 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
EciI GGCGGA 1 cut(s) 185
Eco24I GRGCYC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 2 cut(s) 32, 240
Eco88I CYCGRG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 376
EcoO65I GGTNACC 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 72
FaeI CATG 2 cut(s) 328, 343
FaiI YATR 3 cut(s) 326, 341, 435
FaqI GGGAC 3 cut(s) 45, 127, 253
FatI CATG 2 cut(s) 324, 339
FauI CCCGC 1 cut(s) 22
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 1 cut(s) 121
FokI GGATG 1 cut(s) 242
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 1 cut(s) 121
FspBI CTAG 1 cut(s) 347
GluI GCNGC 1 cut(s) 121
GsaI CCCAGC 1 cut(s) 49
HaeIII GGCC 5 cut(s) 44, 109, 123, 174, 366
HapII CCGG 5 cut(s) 35, 65, 111, 237, 307
HgaI GACGC 1 cut(s) 27
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 2 cut(s) 328, 343
HincII GTYRAC 3 cut(s) 9, 274, 355
HindII GTYRAC 3 cut(s) 9, 274, 355
HinfI GANTC 1 cut(s) 207
HpaII CCGG 5 cut(s) 35, 65, 111, 237, 307
HphI GGTGA 3 cut(s) 244, 388, 454
Hpy166II GTNNAC 3 cut(s) 9, 274, 355
Hpy188I TCNGA 2 cut(s) 74, 214
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 3 cut(s) 9, 274, 355
Hpy99I CGWCG 4 cut(s) 10, 13, 43, 137
HpyAV CCTTC 1 cut(s) 163
HpyCH4III ACNGT 2 cut(s) 160, 298
HpyCH4V TGCA 2 cut(s) 166, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 76
HpyF3I CTNAG 3 cut(s) 104, 186, 315
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 2 cut(s) 328, 343
Kpn2I TCCGGA 1 cut(s) 306
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 5 cut(s) 100, 176, 265, 318, 335
LmnI GCTCC 2 cut(s) 75, 155
MaeI CTAG 1 cut(s) 347
MaeIII GTNAC 1 cut(s) 376
MalI GATC 5 cut(s) 102, 178, 267, 320, 337
MbiI CCGCTC 1 cut(s) 120
MboI GATC 5 cut(s) 100, 176, 265, 318, 335
MboII GAAGA 1 cut(s) 241
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 72
MnlI CCTC 4 cut(s) 203, 230, 336, 367
MroI TCCGGA 1 cut(s) 306
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 5 cut(s) 35, 65, 111, 237, 307
MspR9I CCNGG 4 cut(s) 66, 111, 112, 238
Mva1269I GAATGC 1 cut(s) 316
MwoI GCNNNNNNNGC 2 cut(s) 35, 76
NciI CCSGG 4 cut(s) 66, 111, 112, 238
NdeII GATC 5 cut(s) 100, 176, 265, 318, 335
NheI GCTAGC 1 cut(s) 346
NlaIII CATG 2 cut(s) 328, 343
NlaIV GGNNCC 5 cut(s) 33, 71, 241, 337, 365
NmuCI GTSAC 1 cut(s) 376
NspI RCATGY 1 cut(s) 328
OliI CACNNNNGTG 1 cut(s) 158
PciI ACATGT 1 cut(s) 324
PctI GAATGC 1 cut(s) 316
PfeI GAWTC 1 cut(s) 207
PkrI GCNGC 1 cut(s) 122
Ple19I CGATCG 1 cut(s) 179
PscI ACATGT 1 cut(s) 324
PshAI GACNNNNGTC 1 cut(s) 37
PspEI GGTNACC 1 cut(s) 376
PspFI CCCAGC 1 cut(s) 45
PspN4I GGNNCC 5 cut(s) 33, 71, 241, 337, 365
PspPI GGNCC 6 cut(s) 32, 43, 108, 172, 240, 364
PsuI RGATCY 1 cut(s) 335
PvuI CGATCG 1 cut(s) 179
RseI CAYNNNNRTG 1 cut(s) 158
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 5 cut(s) 100, 176, 265, 318, 335
Sau96I GGNCC 6 cut(s) 32, 43, 108, 172, 240, 364
ScrFI CCNGG 4 cut(s) 66, 111, 112, 238
SduI GDGCHC 1 cut(s) 72
SetI ASST 7 cut(s) 96, 144, 152, 195, 289, 378, 441
SgrDI CGTCGACG 1 cut(s) 7
SinI GGWCC 2 cut(s) 32, 240
SmaI CCCGGG 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 158
SsiI CCGC 4 cut(s) 29, 120, 196, 255
SspMI CTAG 1 cut(s) 347
StyD4I CCNGG 4 cut(s) 64, 109, 110, 236
TaaI ACNGT 2 cut(s) 160, 298
TaqI TCGA 2 cut(s) 8, 321
TaqII GACCGA 1 cut(s) 395
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 1 cut(s) 207
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 1 cut(s) 296
TspMI CCCGGG 1 cut(s) 110
TspRI CASTG 1 cut(s) 301
VpaK11BI GGWCC 2 cut(s) 32, 240
XceI RCATGY 1 cut(s) 328
XmaI CCCGGG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.