MD03G1235100.v1.1

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010659
Physical Location & Seq
Forward (+)
32118489 .. 32120864
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1235100.v1.1.491

Sequence Viewer

Length: 477 bp
ATGTCGACGACCACCAAAACGGCACCGTCTCAGAAACCCCAGCAGAGCAATGCCAAGCCTGGTCTCAGAAAGCCCGTGTTCACCAAGGTCGAGCTGCTCAAGCCGGAGACCGGTGGCCACACGCTCGTCGCCAAGGTTCTGAGCTCCAACCCCGTGCTGCAGAAGGGCCGATCGGTGTCGCAGCACCTTCGCCAGACCCGAATCTCCGAGTGCTTGATTGGGGACGAGACCGGGACCATTCTCTTCACTGCCAGGAACGATCAAGTTGACTTGATGAAGCCTGGTGCAACTGTAATTGTCCGCAATGCAAGGATTGACATGTTCAAGGGGTCGATGAGGCTAGCAGTTGATAAATGGGGGCGTATTGAGGTCACTGAACCTGCAAACTTTGTAGTCAAGGAGGATAACAATCTCTCTTTAGTGGAATATGAGCTGGTGAATGTTCAGGAGGATGCAGGGGCACCGCTTGGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.28

Weight (kDa)

9.33

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 33 - 124 4.2e-44 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 388
AccB1I GGYRCC 2 cut(s) 22, 460
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 301, 464
AcoI YGGCCR 1 cut(s) 115
AfiI CCNNNNNNNGG 1 cut(s) 110
AflIII ACRYGT 1 cut(s) 318
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 1 cut(s) 325
AjnI CCWGG 3 cut(s) 58, 251, 280
AluBI AGCT 4 cut(s) 94, 144, 433, 474
AluI AGCT 4 cut(s) 94, 144, 433, 474
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 4 cut(s) 33, 68, 101, 221
AoxI GGCC 2 cut(s) 115, 166
ApeKI GCWGC 4 cut(s) 94, 157, 181, 471
AsiGI ACCGGT 1 cut(s) 110
AspS9I GGNCC 2 cut(s) 166, 234
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 2 cut(s) 73, 448
AsuNHI GCTAGC 1 cut(s) 340
AvaII GGWCC 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 463
BalI TGGCCA 1 cut(s) 117
BanI GGYRCC 2 cut(s) 22, 460
BanII GRGCYC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 3 cut(s) 81, 144, 193
BceAI ACGGC 1 cut(s) 36
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 3 cut(s) 60, 253, 282
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 4 cut(s) 33, 68, 101, 221
BfaI CTAG 2 cut(s) 341, 475
BfmI CTRYAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 388
BisI GCNGC 4 cut(s) 95, 158, 182, 472
BlsI GCNGC 4 cut(s) 96, 159, 183, 473
Bme1390I CCNGG 4 cut(s) 60, 232, 253, 282
Bme18I GGWCC 1 cut(s) 234
BmgT120I GGNCC 2 cut(s) 166, 234
BmiI GGNNCC 3 cut(s) 24, 235, 462
BmrFI CCNGG 4 cut(s) 60, 232, 253, 282
BmsI GCATC 1 cut(s) 442
BmtI GCTAGC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 232
BsaBI GATNNNNATC 1 cut(s) 408
BsaI GGTCTC 3 cut(s) 68, 101, 221
BsaJI CCNNGG 2 cut(s) 84, 132
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 110
Bse3DI GCAATG 2 cut(s) 55, 310
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 3 cut(s) 60, 253, 282
BseDI CCNNGG 2 cut(s) 84, 132
BseGI GGATG 1 cut(s) 457
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 2 cut(s) 55, 310
BseMII CTCAG 3 cut(s) 44, 79, 131
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 3 cut(s) 81, 144, 193
BseYI CCCAGC 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 173
BshFI GGCC 2 cut(s) 117, 168
BshNI GGYRCC 2 cut(s) 22, 460
BshTI ACCGGT 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 3 cut(s) 104, 111, 231
BslFI GGGAC 2 cut(s) 236, 247
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 4 cut(s) 33, 68, 101, 221
BsmBI CGTCTC 1 cut(s) 33
BsmFI GGGAC 2 cut(s) 236, 247
BsnI GGCC 2 cut(s) 117, 168
Bso31I GGTCTC 3 cut(s) 68, 101, 221
Bsp1286I GDGCHC 2 cut(s) 146, 463
Bsp143I GATC 2 cut(s) 170, 259
BspACI CCGC 2 cut(s) 301, 464
BspANI GGCC 2 cut(s) 117, 168
BspCNI CTCAG 3 cut(s) 43, 78, 132
BspLI GGNNCC 3 cut(s) 24, 235, 462
BspMAI CTGCAG 1 cut(s) 162
BspMI ACCTGC 1 cut(s) 388
BspOI GCTAGC 1 cut(s) 344
BspT107I GGYRCC 2 cut(s) 22, 460
BspTNI GGTCTC 3 cut(s) 68, 101, 221
BsrDI GCAATG 2 cut(s) 55, 310
BsrFI RCCGGY 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 84, 132
BssMI GATC 2 cut(s) 170, 259
BssT1I CCWWGG 2 cut(s) 84, 132
Bst2UI CCWGG 3 cut(s) 60, 253, 282
Bst4CI ACNGT 2 cut(s) 27, 292
Bst6I CTCTTC 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 3 cut(s) 30, 65, 140
BstF5I GGATG 1 cut(s) 457
BstKTI GATC 2 cut(s) 173, 262
BstMAI GTCTC 4 cut(s) 33, 68, 101, 221
BstMBI GATC 2 cut(s) 170, 259
BstMCI CGRYCG 1 cut(s) 173
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 3 cut(s) 60, 253, 282
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 4 cut(s) 58, 230, 251, 280
BstSFI CTRYAG 1 cut(s) 158
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 3 cut(s) 81, 144, 193
BsuRI GGCC 2 cut(s) 117, 168
BtsCI GGATG 1 cut(s) 457
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 246, 372
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 342
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 166, 234
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 1 cut(s) 319
DdeI CTNAG 3 cut(s) 30, 65, 140
DpnI GATC 2 cut(s) 172, 261
DpnII GATC 2 cut(s) 170, 259
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 84, 132
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 3 cut(s) 68, 101, 221
Eco47I GGWCC 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 144
EcoRII CCWGG 3 cut(s) 58, 251, 280
EcoT14I CCWWGG 2 cut(s) 84, 132
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 84, 132
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 1 cut(s) 322
FaiI YATR 2 cut(s) 320, 429
FaqI GGGAC 2 cut(s) 236, 247
FatI CATG 1 cut(s) 318
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 95, 158, 182, 472
FokI GGATG 1 cut(s) 464
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 4 cut(s) 95, 158, 182, 472
FspBI CTAG 2 cut(s) 341, 475
GluI GCNGC 4 cut(s) 95, 158, 182, 472
GsaI CCCAGC 1 cut(s) 43
HaeIII GGCC 2 cut(s) 117, 168
HapII CCGG 3 cut(s) 104, 111, 231
Hin1II CATG 1 cut(s) 322
HincII GTYRAC 2 cut(s) 6, 268
HindII GTYRAC 2 cut(s) 6, 268
HinfI GANTC 1 cut(s) 201
HpaII CCGG 3 cut(s) 104, 111, 231
HphI GGTGA 2 cut(s) 73, 448
Hpy166II GTNNAC 3 cut(s) 6, 81, 268
Hpy188I TCNGA 4 cut(s) 33, 68, 141, 208
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 3 cut(s) 6, 81, 268
Hpy99I CGWCG 2 cut(s) 10, 131
HpyAV CCTTC 2 cut(s) 157, 197
HpyCH4III ACNGT 2 cut(s) 27, 292
HpyCH4V TGCA 5 cut(s) 160, 287, 308, 383, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpyF3I CTNAG 3 cut(s) 30, 65, 140
Hsp92II CATG 1 cut(s) 322
Kzo9I GATC 2 cut(s) 170, 259
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 3 cut(s) 81, 144, 193
LweI GCATC 1 cut(s) 442
MaeI CTAG 2 cut(s) 341, 475
MaeIII GTNAC 1 cut(s) 370
MalI GATC 2 cut(s) 172, 261
MboI GATC 2 cut(s) 170, 259
MboII GAAGA 1 cut(s) 235
MhlI GDGCHC 2 cut(s) 146, 463
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 1 cut(s) 294
MluNI TGGCCA 1 cut(s) 117
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 4 cut(s) 330, 361, 394, 442
Mox20I TGGCCA 1 cut(s) 117
MscI TGGCCA 1 cut(s) 117
Msp20I TGGCCA 1 cut(s) 117
MspI CCGG 3 cut(s) 104, 111, 231
MspR9I CCNGG 4 cut(s) 60, 232, 253, 282
MvaI CCWGG 3 cut(s) 60, 253, 282
MwoI GCNNNNNNNGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 232
NdeII GATC 2 cut(s) 170, 259
NheI GCTAGC 1 cut(s) 340
NlaIII CATG 1 cut(s) 322
NlaIV GGNNCC 3 cut(s) 24, 235, 462
NmuCI GTSAC 1 cut(s) 370
NspI RCATGY 1 cut(s) 322
PciI ACATGT 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PfeI GAWTC 1 cut(s) 201
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 4 cut(s) 96, 159, 183, 473
Ple19I CGATCG 1 cut(s) 173
PscI ACATGT 1 cut(s) 318
Psp124BI GAGCTC 1 cut(s) 146
Psp6I CCWGG 3 cut(s) 58, 251, 280
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 3 cut(s) 58, 251, 280
PspN4I GGNNCC 3 cut(s) 24, 235, 462
PspPI GGNCC 2 cut(s) 166, 234
PstI CTGCAG 1 cut(s) 162
PvuI CGATCG 1 cut(s) 173
SacI GAGCTC 1 cut(s) 146
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 4 cut(s) 95, 158, 182, 472
Sau3AI GATC 2 cut(s) 170, 259
Sau96I GGNCC 2 cut(s) 166, 234
ScrFI CCNGG 4 cut(s) 60, 232, 253, 282
SduI GDGCHC 2 cut(s) 146, 463
SetI ASST 9 cut(s) 90, 96, 138, 146, 189, 372, 382, 435, 476
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 158
SinI GGWCC 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 1 cut(s) 294
SsiI CCGC 2 cut(s) 301, 464
SspMI CTAG 2 cut(s) 341, 475
SstI GAGCTC 1 cut(s) 146
StyD4I CCNGG 4 cut(s) 58, 230, 251, 280
StyI CCWWGG 2 cut(s) 84, 132
TaaI ACNGT 2 cut(s) 27, 292
TaqI TCGA 3 cut(s) 5, 90, 332
TasI AATT 1 cut(s) 294
TfiI GAWTC 1 cut(s) 201
TscAI CASTG 2 cut(s) 253, 379
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 4 cut(s) 94, 157, 181, 471
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 290
TspRI CASTG 2 cut(s) 253, 379
VpaK11BI GGWCC 1 cut(s) 234
XceI RCATGY 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 341, 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.