Rw5G020520

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010659
Physical Location & Seq
Reverse (-)
27014156 .. 27016547
2392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020520.1

Sequence Viewer

Length: 465 bp
ATGTCGTCGACGACCACCACCACCAACAAAGCGGGACCGGCGTCGGCCCAGCAGAGCAATGCGAAGCCGGGGCTCCGAAAGCCCGTGTTCGTCAAGGTCAGCGATCTCAGGCCCGGGACGAGCGGCCACACTCTCGTCGTCAAGGTCATCAGCTCCACCTCCGTGTTGCAGAAGGGCCGATCGGTGTCTCAGCACCTCCGCCAGACCCGAATCTCCGAGTGCCTCATTGGGGATGAGACCGGGACCATTCTCTTCACCGCCAGAAATGATCAAGTTGACTTGATGAAACCTGATACCACTGTTATTCTCCGGAATGCTAAGATCGACATGTTTAAGGGATCCATGAGGCTAGCAGTTGACAAATGGGGCCGTATTGAGGTGACCGAGCCTGCAAACTTTGTAGTCAAGGAGAACAACAATCTCTCTTTAGTTGAATATGAGCTGGTGAGTGTTGTAGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

16.86

Weight (kDa)

9.37

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 36 - 127 1.5e-45 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 309
AciI CCGC 4 cut(s) 32, 123, 199, 258
AclWI GGATC 2 cut(s) 333, 346
AcoI YGGCCR 1 cut(s) 124
AcyI GRCGYC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 229, 376
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 1 cut(s) 434
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 2 cut(s) 153, 442
AluI AGCT 2 cut(s) 153, 442
Alw26I GTCTC 2 cut(s) 192, 230
AlwI GGATC 2 cut(s) 333, 346
Ama87I CYCGRG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 309
AoxI GGCC 5 cut(s) 45, 110, 124, 175, 367
AspS9I GGNCC 6 cut(s) 35, 46, 111, 175, 243, 367
AsuC2I CCSGG 4 cut(s) 69, 114, 115, 241
AsuHPI GGTGA 3 cut(s) 247, 391, 457
AsuNHI GCTAGC 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 2 cut(s) 35, 243
BaeI ACNNNNGTAYC 2 cut(s) 285, 318
BamHI GGATCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 75
BceAI ACGGC 1 cut(s) 354
BclI TGATCA 1 cut(s) 268
BcnI CCSGG 4 cut(s) 69, 114, 115, 241
BcoDI GTCTC 2 cut(s) 192, 230
BfaI CTAG 1 cut(s) 350
BisI GCNGC 1 cut(s) 124
BlsI GCNGC 1 cut(s) 125
Bme1390I CCNGG 4 cut(s) 69, 114, 115, 241
Bme18I GGWCC 2 cut(s) 35, 243
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 6 cut(s) 35, 46, 111, 175, 243, 367
BmiI GGNNCC 5 cut(s) 36, 74, 244, 340, 368
BmrFI CCNGG 4 cut(s) 69, 114, 115, 241
BmtI GCTAGC 1 cut(s) 353
BoxI GACNNNNGTC 1 cut(s) 40
BpuMI CCSGG 4 cut(s) 69, 114, 115, 241
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 68, 113
BsaWI WCCGGW 1 cut(s) 309
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 376
Bse118I RCCGGY 1 cut(s) 37
Bse3DI GCAATG 1 cut(s) 64
BseAI TCCGGA 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 68, 113
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 2 cut(s) 229, 376
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 2 cut(s) 121, 203
BseYI CCCAGC 1 cut(s) 48
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 5 cut(s) 47, 112, 126, 177, 369
BsiEI CGRYCG 1 cut(s) 182
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 5 cut(s) 38, 68, 114, 240, 310
BslFI GGGAC 3 cut(s) 48, 130, 256
BslI CCNNNNNNNGG 2 cut(s) 229, 376
BsmAI GTCTC 2 cut(s) 192, 230
BsmFI GGGAC 3 cut(s) 48, 130, 256
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 5 cut(s) 47, 112, 126, 177, 369
Bso31I GGTCTC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 75
Bsp13I TCCGGA 1 cut(s) 309
Bsp143I GATC 5 cut(s) 103, 179, 268, 321, 338
BspACI CCGC 4 cut(s) 32, 123, 199, 258
BspANI GGCC 5 cut(s) 47, 112, 126, 177, 369
BspCNI CTCAG 2 cut(s) 120, 202
BspEI TCCGGA 1 cut(s) 309
BspLI GGNNCC 5 cut(s) 36, 74, 244, 340, 368
BspOI GCTAGC 1 cut(s) 353
BspPI GGATC 2 cut(s) 333, 346
BspTNI GGTCTC 1 cut(s) 230
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 64
BsrFI RCCGGY 1 cut(s) 37
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 68, 113
BssMI GATC 5 cut(s) 103, 179, 268, 321, 338
BssNI GRCGYC 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 301
Bst6I CTCTTC 1 cut(s) 257
BstACI GRCGYC 1 cut(s) 41
BstC8I GCNNGC 2 cut(s) 351, 390
BstDEI CTNAG 3 cut(s) 107, 189, 318
BstEII GGTNACC 1 cut(s) 379
BstF5I GGATG 1 cut(s) 238
BstKTI GATC 5 cut(s) 106, 182, 271, 324, 341
BstMAI GTCTC 2 cut(s) 192, 230
BstMBI GATC 5 cut(s) 103, 179, 268, 321, 338
BstMCI CGRYCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 2 cut(s) 38, 79
BstNSI RCATGY 1 cut(s) 331
BstPAI GACNNNNGTC 1 cut(s) 40
BstPI GGTNACC 1 cut(s) 379
BstSCI CCNGG 4 cut(s) 67, 112, 113, 239
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BsuRI GGCC 5 cut(s) 47, 112, 126, 177, 369
BtsCI GGATG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 2 cut(s) 351, 390
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 6 cut(s) 35, 46, 111, 175, 243, 367
Cfr9I CCCGGG 1 cut(s) 113
CseI GACGC 1 cut(s) 30
CviAII CATG 2 cut(s) 328, 343
DdeI CTNAG 3 cut(s) 107, 189, 318
DpnI GATC 5 cut(s) 105, 181, 270, 323, 340
DpnII GATC 5 cut(s) 103, 179, 268, 321, 338
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
EciI GGCGGA 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 35, 243
Eco88I CYCGRG 1 cut(s) 113
Eco91I GGTNACC 1 cut(s) 379
EcoO65I GGTNACC 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 75
FaeI CATG 2 cut(s) 331, 346
FaiI YATR 3 cut(s) 329, 344, 438
FaqI GGGAC 3 cut(s) 48, 130, 256
FatI CATG 2 cut(s) 327, 342
FauI CCCGC 1 cut(s) 25
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 1 cut(s) 124
FokI GGATG 1 cut(s) 245
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 124
FspBI CTAG 1 cut(s) 350
GluI GCNGC 1 cut(s) 124
GsaI CCCAGC 1 cut(s) 52
HaeIII GGCC 5 cut(s) 47, 112, 126, 177, 369
HapII CCGG 5 cut(s) 38, 68, 114, 240, 310
HgaI GACGC 1 cut(s) 30
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 2 cut(s) 331, 346
HincII GTYRAC 3 cut(s) 9, 277, 358
HindII GTYRAC 3 cut(s) 9, 277, 358
HinfI GANTC 1 cut(s) 210
HpaII CCGG 5 cut(s) 38, 68, 114, 240, 310
HphI GGTGA 3 cut(s) 247, 391, 457
Hpy166II GTNNAC 3 cut(s) 9, 277, 358
Hpy188I TCNGA 2 cut(s) 77, 217
Hpy188III TCNNGA 1 cut(s) 310
Hpy8I GTNNAC 3 cut(s) 9, 277, 358
Hpy99I CGWCG 4 cut(s) 10, 13, 46, 140
HpyAV CCTTC 1 cut(s) 166
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4V TGCA 2 cut(s) 169, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 79
HpyF3I CTNAG 3 cut(s) 107, 189, 318
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 2 cut(s) 331, 346
Kpn2I TCCGGA 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 5 cut(s) 103, 179, 268, 321, 338
LmnI GCTCC 2 cut(s) 78, 158
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 1 cut(s) 379
MalI GATC 5 cut(s) 105, 181, 270, 323, 340
MbiI CCGCTC 1 cut(s) 123
MboI GATC 5 cut(s) 103, 179, 268, 321, 338
MboII GAAGA 1 cut(s) 244
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 1 cut(s) 75
MnlI CCTC 5 cut(s) 169, 206, 233, 339, 370
MroI TCCGGA 1 cut(s) 309
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 5 cut(s) 38, 68, 114, 240, 310
MspR9I CCNGG 4 cut(s) 69, 114, 115, 241
Mva1269I GAATGC 1 cut(s) 319
MwoI GCNNNNNNNGC 2 cut(s) 38, 79
NciI CCSGG 4 cut(s) 69, 114, 115, 241
NdeII GATC 5 cut(s) 103, 179, 268, 321, 338
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 2 cut(s) 331, 346
NlaIV GGNNCC 5 cut(s) 36, 74, 244, 340, 368
NmuCI GTSAC 1 cut(s) 379
NspI RCATGY 1 cut(s) 331
OliI CACNNNNGTG 1 cut(s) 161
PciI ACATGT 1 cut(s) 327
PctI GAATGC 1 cut(s) 319
PfeI GAWTC 1 cut(s) 210
PkrI GCNGC 1 cut(s) 125
Ple19I CGATCG 1 cut(s) 182
PscI ACATGT 1 cut(s) 327
PshAI GACNNNNGTC 1 cut(s) 40
PspEI GGTNACC 1 cut(s) 379
PspFI CCCAGC 1 cut(s) 48
PspN4I GGNNCC 5 cut(s) 36, 74, 244, 340, 368
PspPI GGNCC 6 cut(s) 35, 46, 111, 175, 243, 367
PsuI RGATCY 1 cut(s) 338
PvuI CGATCG 1 cut(s) 182
RseI CAYNNNNRTG 1 cut(s) 161
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 1 cut(s) 124
Sau3AI GATC 5 cut(s) 103, 179, 268, 321, 338
Sau96I GGNCC 6 cut(s) 35, 46, 111, 175, 243, 367
ScrFI CCNGG 4 cut(s) 69, 114, 115, 241
SduI GDGCHC 1 cut(s) 75
SetI ASST 8 cut(s) 99, 147, 155, 161, 198, 292, 381, 444
SgrDI CGTCGACG 1 cut(s) 7
SinI GGWCC 2 cut(s) 35, 243
SmaI CCCGGG 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 161
SsiI CCGC 4 cut(s) 32, 123, 199, 258
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 4 cut(s) 67, 112, 113, 239
TaaI ACNGT 1 cut(s) 301
TaqI TCGA 2 cut(s) 8, 324
TaqII GACCGA 1 cut(s) 398
TauI GCSGC 1 cut(s) 126
TfiI GAWTC 1 cut(s) 210
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 1 cut(s) 151
TspMI CCCGGG 1 cut(s) 113
TspRI CASTG 1 cut(s) 304
VpaK11BI GGWCC 2 cut(s) 35, 243
XceI RCATGY 1 cut(s) 331
XmaI CCCGGG 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.