MD11G1255600.v1.1

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010659
Physical Location & Seq
Forward (+)
36831611 .. 36834303
2693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1255600.v1.1.491

Sequence Viewer

Length: 477 bp
ATGTCGACGTCCACCAAAACGGCACCGTCTCAGACACCCCAGCAGAGCAATGCCAAGCCTGGTCTCAGGAAGCCCGTGTTCACCAAGGTCGAGTTGCTCAAGCCGGAGACCAGCGGCCACACGCTCGTCGCCAAGGTCCTGAGCTCGAACCCTGTGCTGCAGAAGGGCCGATCGGTGTCGCAGCACCTTCGCCAGACTCGAATCTCTGAGTGCTTGATTGGGGACGAGACTGGGACCATTCTCTTCACTGCCAGGAACGATCAAGTTGACTTGATGAAGCCTGGTGCAACTGTAATTCTCCGCAATGCAAGGATTGACATGTTCAAGGGATCGATGAGGCTAGCAGTTGATAAATGGGGACGTATTGAGGTCACTGAACCTGCAAACTTTGTAGTCAAAGAGGATAACAATCTCTCTCTAGTGGAATATGAGCTGGTGAATGTTCAGGAGGAAGGAGGGGCGCCGCCTGGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.3

Weight (kDa)

9.13

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 33 - 124 8.5e-45 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 388
AccB1I GGYRCC 2 cut(s) 22, 460
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 114, 301, 464
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 8, 461
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 1 cut(s) 325
AjnI CCWGG 4 cut(s) 58, 251, 280, 466
AluBI AGCT 2 cut(s) 144, 433
AluI AGCT 2 cut(s) 144, 433
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 4 cut(s) 33, 68, 101, 221
AlwI GGATC 1 cut(s) 337
AoxI GGCC 2 cut(s) 115, 166
ApeKI GCWGC 2 cut(s) 157, 181
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 3 cut(s) 136, 166, 234
AsuHPI GGTGA 2 cut(s) 73, 448
AsuNHI GCTAGC 1 cut(s) 340
AvaII GGWCC 2 cut(s) 136, 234
BanI GGYRCC 2 cut(s) 22, 460
BanII GRGCYC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 2 cut(s) 144, 193
BceAI ACGGC 1 cut(s) 36
BcgI CGANNNNNNTGC 4 cut(s) 136, 170, 170, 204
BciT130I CCWGG 4 cut(s) 60, 253, 282, 468
BcoDI GTCTC 4 cut(s) 33, 68, 101, 221
BfaI CTAG 2 cut(s) 341, 419
BfmI CTRYAG 1 cut(s) 158
BfoI RGCGCY 1 cut(s) 464
BfuAI ACCTGC 1 cut(s) 388
BisI GCNGC 4 cut(s) 115, 158, 182, 464
BlsI GCNGC 4 cut(s) 116, 159, 183, 465
Bme1390I CCNGG 4 cut(s) 60, 253, 282, 468
Bme18I GGWCC 2 cut(s) 136, 234
BmgT120I GGNCC 3 cut(s) 136, 166, 234
BmiI GGNNCC 3 cut(s) 24, 235, 462
BmrFI CCNGG 4 cut(s) 60, 253, 282, 468
BmrI ACTGGG 1 cut(s) 240
BmtI GCTAGC 1 cut(s) 344
BmuI ACTGGG 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 83
Bsa29I ATCGAT 1 cut(s) 332
BsaBI GATNNNNATC 1 cut(s) 408
BsaHI GRCGYC 2 cut(s) 8, 461
BsaI GGTCTC 2 cut(s) 68, 101
BsaJI CCNNGG 2 cut(s) 84, 132
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 2 cut(s) 55, 310
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 4 cut(s) 60, 253, 282, 468
BseCI ATCGAT 1 cut(s) 332
BseDI CCNNGG 2 cut(s) 84, 132
BseJI GATNNNNATC 1 cut(s) 408
BseMI GCAATG 2 cut(s) 55, 310
BseMII CTCAG 4 cut(s) 44, 79, 131, 198
BseNI ACTGG 1 cut(s) 235
BseXI GCAGC 2 cut(s) 144, 193
BseYI CCCAGC 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 173
BshFI GGCC 2 cut(s) 117, 168
BshNI GGYRCC 2 cut(s) 22, 460
BshVI ATCGAT 1 cut(s) 332
BsiEI CGRYCG 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 3 cut(s) 236, 247, 372
BsmAI GTCTC 4 cut(s) 33, 68, 101, 221
BsmBI CGTCTC 1 cut(s) 33
BsmFI GGGAC 3 cut(s) 236, 247, 372
BsnI GGCC 2 cut(s) 117, 168
Bso31I GGTCTC 2 cut(s) 68, 101
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 3 cut(s) 170, 259, 329
BspACI CCGC 3 cut(s) 114, 301, 464
BspANI GGCC 2 cut(s) 117, 168
BspCNI CTCAG 4 cut(s) 43, 78, 132, 199
BspDI ATCGAT 1 cut(s) 332
BspLI GGNNCC 3 cut(s) 24, 235, 462
BspMAI CTGCAG 1 cut(s) 162
BspMI ACCTGC 1 cut(s) 388
BspOI GCTAGC 1 cut(s) 344
BspPI GGATC 1 cut(s) 337
BspT107I GGYRCC 2 cut(s) 22, 460
BspTNI GGTCTC 2 cut(s) 68, 101
BsrDI GCAATG 2 cut(s) 55, 310
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 2 cut(s) 84, 132
BssMI GATC 3 cut(s) 170, 259, 329
BssNI GRCGYC 2 cut(s) 8, 461
BssT1I CCWWGG 2 cut(s) 84, 132
Bst2UI CCWGG 4 cut(s) 60, 253, 282, 468
Bst4CI ACNGT 2 cut(s) 27, 292
Bst6I CTCTTC 1 cut(s) 248
BstACI GRCGYC 2 cut(s) 8, 461
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 4 cut(s) 30, 65, 140, 207
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 3 cut(s) 173, 262, 332
BstMAI GTCTC 4 cut(s) 33, 68, 101, 221
BstMBI GATC 3 cut(s) 170, 259, 329
BstMCI CGRYCG 1 cut(s) 173
BstNI CCWGG 4 cut(s) 60, 253, 282, 468
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 4 cut(s) 58, 251, 280, 466
BstSFI CTRYAG 1 cut(s) 158
BstV1I GCAGC 2 cut(s) 144, 193
Bsu15I ATCGAT 1 cut(s) 332
BsuRI GGCC 2 cut(s) 117, 168
BsuTUI ATCGAT 1 cut(s) 332
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 246, 372
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 342
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 3 cut(s) 136, 166, 234
ClaI ATCGAT 1 cut(s) 332
CviAII CATG 1 cut(s) 319
CviJI RGCY 9 cut(s) 58, 73, 103, 117, 144, 168, 280, 340, 433
CviKI_1 RGCY 9 cut(s) 58, 73, 103, 117, 144, 168, 280, 340, 433
DdeI CTNAG 4 cut(s) 30, 65, 140, 207
DinI GGCGCC 1 cut(s) 462
DpnI GATC 3 cut(s) 172, 261, 331
DpnII GATC 3 cut(s) 170, 259, 329
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 84, 132
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 2 cut(s) 68, 101
Eco47I GGWCC 2 cut(s) 136, 234
Eco53kI GAGCTC 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 4 cut(s) 58, 251, 280, 466
EcoT14I CCWWGG 2 cut(s) 84, 132
EcoT38I GRGCYC 1 cut(s) 146
EgeI GGCGCC 1 cut(s) 462
EheI GGCGCC 1 cut(s) 462
ErhI CCWWGG 2 cut(s) 84, 132
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 1 cut(s) 322
FaiI YATR 2 cut(s) 320, 429
FaqI GGGAC 3 cut(s) 236, 247, 372
FatI CATG 1 cut(s) 318
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 115, 158, 182, 464
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 4 cut(s) 115, 158, 182, 464
FspBI CTAG 2 cut(s) 341, 419
GlaI GCGC 1 cut(s) 462
GluI GCNGC 4 cut(s) 115, 158, 182, 464
GsaI CCCAGC 1 cut(s) 43
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 2 cut(s) 117, 168
HapII CCGG 1 cut(s) 104
HhaI GCGC 1 cut(s) 463
Hin1I GRCGYC 2 cut(s) 8, 461
Hin1II CATG 1 cut(s) 322
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HincII GTYRAC 2 cut(s) 6, 268
HindII GTYRAC 2 cut(s) 6, 268
HinfI GANTC 2 cut(s) 196, 201
HpaII CCGG 1 cut(s) 104
HphI GGTGA 2 cut(s) 73, 448
Hpy166II GTNNAC 4 cut(s) 6, 12, 81, 268
Hpy188I TCNGA 2 cut(s) 33, 208
Hpy188III TCNNGA 3 cut(s) 67, 139, 446
Hpy8I GTNNAC 4 cut(s) 6, 12, 81, 268
Hpy99I CGWCG 2 cut(s) 10, 131
HpyAV CCTTC 3 cut(s) 157, 197, 446
HpyCH4III ACNGT 2 cut(s) 27, 292
HpyCH4IV ACGT 2 cut(s) 8, 361
HpyCH4V TGCA 4 cut(s) 160, 287, 308, 383
HpyF3I CTNAG 4 cut(s) 30, 65, 140, 207
HpySE526I ACGT 2 cut(s) 8, 361
Hsp92I GRCGYC 2 cut(s) 8, 461
Hsp92II CATG 1 cut(s) 322
HspAI GCGC 1 cut(s) 461
KasI GGCGCC 1 cut(s) 460
Kzo9I GATC 3 cut(s) 170, 259, 329
Lsp1109I GCAGC 2 cut(s) 144, 193
MaeI CTAG 2 cut(s) 341, 419
MaeII ACGT 2 cut(s) 8, 361
MaeIII GTNAC 1 cut(s) 370
MalI GATC 3 cut(s) 172, 261, 331
MboI GATC 3 cut(s) 170, 259, 329
MboII GAAGA 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 2 cut(s) 294, 472
Mly113I GGCGCC 1 cut(s) 461
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 5 cut(s) 330, 361, 394, 442, 449
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 4 cut(s) 60, 253, 282, 468
MvaI CCWGG 4 cut(s) 60, 253, 282, 468
NarI GGCGCC 1 cut(s) 461
NdeII GATC 3 cut(s) 170, 259, 329
NheI GCTAGC 1 cut(s) 340
NlaIII CATG 1 cut(s) 322
NlaIV GGNNCC 3 cut(s) 24, 235, 462
NmuCI GTSAC 1 cut(s) 370
NspI RCATGY 1 cut(s) 322
PciI ACATGT 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PfeI GAWTC 1 cut(s) 201
PkrI GCNGC 4 cut(s) 116, 159, 183, 465
Ple19I CGATCG 1 cut(s) 173
PleI GAGTC 1 cut(s) 190
PluTI GGCGCC 1 cut(s) 464
PpsI GAGTC 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 318
Psp124BI GAGCTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 4 cut(s) 58, 251, 280, 466
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 4 cut(s) 58, 251, 280, 466
PspN4I GGNNCC 3 cut(s) 24, 235, 462
PspPI GGNCC 3 cut(s) 136, 166, 234
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 162
PvuI CGATCG 1 cut(s) 173
SacI GAGCTC 1 cut(s) 146
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 4 cut(s) 115, 158, 182, 464
Sau3AI GATC 3 cut(s) 170, 259, 329
Sau96I GGNCC 3 cut(s) 136, 166, 234
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 4 cut(s) 60, 253, 282, 468
SduI GDGCHC 1 cut(s) 146
SetI ASST 9 cut(s) 11, 90, 138, 146, 189, 364, 372, 382, 435
SfcI CTRYAG 1 cut(s) 158
SfoI GGCGCC 1 cut(s) 462
SinI GGWCC 2 cut(s) 136, 234
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 2 cut(s) 294, 472
SsiI CCGC 3 cut(s) 114, 301, 464
SspDI GGCGCC 1 cut(s) 460
SspMI CTAG 2 cut(s) 341, 419
SstI GAGCTC 1 cut(s) 146
StyD4I CCNGG 4 cut(s) 58, 251, 280, 466
StyI CCWWGG 2 cut(s) 84, 132
TaaI ACNGT 2 cut(s) 27, 292
TaiI ACGT 2 cut(s) 11, 364
TaqI TCGA 5 cut(s) 5, 90, 146, 199, 332
TasI AATT 2 cut(s) 294, 472
TauI GCSGC 2 cut(s) 117, 466
TfiI GAWTC 1 cut(s) 201
TscAI CASTG 2 cut(s) 253, 379
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 2 cut(s) 157, 181
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 290
TspRI CASTG 2 cut(s) 253, 379
VpaK11BI GGWCC 2 cut(s) 136, 234
XceI RCATGY 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 341, 419
ZraI GACGTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.