Prupe.4G174100_v2.0.a1

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0010659
Physical Location & Seq
Reverse (-)
10306859 .. 10309491
2633 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G174100.1

Sequence Viewer

Length: 480 bp
ATGTCGACGGTCACCAACAAAACGGCACCGTCTCAGCAACCCCAGCAGAGCAATGCCAAGCCAGGTCTCAGGAAGCCCGTCTTCACCAAGGTCGAGCTCCTCAAGCCGGAGACCAGTGGACACACCCTCGTCGCTAAGGTCCTCACCTCCAACCCTGTCTTGCAAAAGGGCCGATCGGTCTCTCAGCACCTCCGCCAGACCCGAATCTCTGAGTGCCTCATTGGGGACGAGACTGGGACTATTCTCTTCACTGCTAGGAATGATCAAGTTGACTTGTTGAAGCCTGGTACAACTGTTATTCTCCGCAATGCAAGGATTGACATGTTCAAAGGATCTATGAGGCTAGCAGTTGATAAATGGGGCCGTATTGAGGTTACTGAACCTGCAAATTTTGTAGTCAAGGAGGATAACAATCTGTCTTTAGTTGAATATGAGCTGGTGAACGTTCAGGAGGATGGAGGGGCTCCGCCTGGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.44

Weight (kDa)

9.13

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 193, 304, 467
AclI AACGTT 1 cut(s) 444
AclWI GGATC 1 cut(s) 340
AcsI RAATTY 1 cut(s) 388
AfaI GTAC 1 cut(s) 289
AfiI CCNNNNNNNGG 3 cut(s) 106, 223, 370
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 3 cut(s) 280, 328, 428
AjnI CCWGG 3 cut(s) 61, 283, 469
AluBI AGCT 2 cut(s) 97, 436
AluI AGCT 2 cut(s) 97, 436
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 5 cut(s) 36, 71, 104, 184, 224
AlwI GGATC 1 cut(s) 340
AoxI GGCC 2 cut(s) 169, 361
ApoI RAATTY 1 cut(s) 388
AspS9I GGNCC 3 cut(s) 139, 169, 361
AsuHPI GGTGA 4 cut(s) 4, 76, 136, 451
AsuNHI GCTAGC 1 cut(s) 343
AvaII GGWCC 1 cut(s) 139
BaeI ACNNNNGTAYC 2 cut(s) 279, 312
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 2 cut(s) 99, 466
BbsI GAAGAC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 99
BccI CCATC 1 cut(s) 449
BceAI ACGGC 2 cut(s) 39, 348
BciT130I CCWGG 3 cut(s) 63, 285, 471
BclI TGATCA 1 cut(s) 262
BcoDI GTCTC 5 cut(s) 36, 71, 104, 184, 224
BfaI CTAG 2 cut(s) 255, 344
BfuAI ACCTGC 1 cut(s) 391
Bme1390I CCNGG 3 cut(s) 63, 285, 471
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 3 cut(s) 139, 169, 361
BmiI GGNNCC 3 cut(s) 27, 362, 465
BmrFI CCNGG 3 cut(s) 63, 285, 471
BmrI ACTGGG 1 cut(s) 243
BmtI GCTAGC 1 cut(s) 347
BmuI ACTGGG 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 73
Bpu10I CCTNAGC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 411
BsaI GGTCTC 3 cut(s) 71, 104, 184
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 223, 370
Bse1I ACTGG 2 cut(s) 114, 238
Bse3DI GCAATG 2 cut(s) 58, 313
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 3 cut(s) 63, 285, 471
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 460
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 3 cut(s) 106, 223, 370
BseMI GCAATG 2 cut(s) 58, 313
BseMII CTCAG 4 cut(s) 47, 82, 197, 201
BseNI ACTGG 2 cut(s) 114, 238
BseRI GAGGAG 1 cut(s) 89
BseYI CCCAGC 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 2 cut(s) 171, 363
BshNI GGYRCC 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 107
BslFI GGGAC 2 cut(s) 239, 250
BslI CCNNNNNNNGG 3 cut(s) 106, 223, 370
BsmAI GTCTC 5 cut(s) 36, 71, 104, 184, 224
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 2 cut(s) 239, 250
BsnI GGCC 2 cut(s) 171, 363
Bso31I GGTCTC 3 cut(s) 71, 104, 184
Bsp1286I GDGCHC 2 cut(s) 99, 466
Bsp143I GATC 3 cut(s) 173, 262, 332
BspACI CCGC 3 cut(s) 193, 304, 467
BspANI GGCC 2 cut(s) 171, 363
BspCNI CTCAG 4 cut(s) 46, 81, 196, 202
BspLI GGNNCC 3 cut(s) 27, 362, 465
BspMI ACCTGC 1 cut(s) 391
BspOI GCTAGC 1 cut(s) 347
BspPI GGATC 1 cut(s) 340
BspT107I GGYRCC 1 cut(s) 25
BspTNI GGTCTC 3 cut(s) 71, 104, 184
BsrDI GCAATG 2 cut(s) 58, 313
BsrI ACTGG 2 cut(s) 114, 238
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 3 cut(s) 173, 262, 332
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 3 cut(s) 63, 285, 471
Bst4CI ACNGT 3 cut(s) 10, 30, 295
Bst6I CTCTTC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 5 cut(s) 33, 68, 135, 183, 210
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 3 cut(s) 176, 265, 335
BstMAI GTCTC 5 cut(s) 36, 71, 104, 184, 224
BstMBI GATC 3 cut(s) 173, 262, 332
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 2 cut(s) 43, 103
BstNI CCWGG 3 cut(s) 63, 285, 471
BstNSI RCATGY 1 cut(s) 325
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 3 cut(s) 61, 283, 469
BstV2I GAAGAC 1 cut(s) 73
BstX2I RGATCY 1 cut(s) 332
BstYI RGATCY 1 cut(s) 332
BsuRI GGCC 2 cut(s) 171, 363
BtsCI GGATG 1 cut(s) 460
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 121, 249
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 345
Cfr13I GGNCC 3 cut(s) 139, 169, 361
Csp6I GTAC 1 cut(s) 288
CviAII CATG 1 cut(s) 322
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 5 cut(s) 33, 68, 135, 183, 210
DpnI GATC 3 cut(s) 175, 264, 334
DpnII GATC 3 cut(s) 173, 262, 332
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 2 cut(s) 182, 456
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 2 cut(s) 99, 466
Eco31I GGTCTC 3 cut(s) 71, 104, 184
Eco47I GGWCC 1 cut(s) 139
Eco53kI GAGCTC 1 cut(s) 97
Eco91I GGTNACC 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 139
EcoO65I GGTNACC 1 cut(s) 10
EcoRII CCWGG 3 cut(s) 61, 283, 469
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 2 cut(s) 99, 466
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 1 cut(s) 325
FaiI YATR 3 cut(s) 323, 338, 432
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FaqI GGGAC 2 cut(s) 239, 250
FatI CATG 1 cut(s) 321
FbaI TGATCA 1 cut(s) 262
FblI GTMKAC 1 cut(s) 5
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 2 cut(s) 99, 466
FspBI CTAG 2 cut(s) 255, 344
GsaI CCCAGC 1 cut(s) 46
HaeIII GGCC 2 cut(s) 171, 363
HapII CCGG 1 cut(s) 107
Hin1II CATG 1 cut(s) 325
HincII GTYRAC 2 cut(s) 6, 271
HindII GTYRAC 2 cut(s) 6, 271
HinfI GANTC 1 cut(s) 204
HpaII CCGG 1 cut(s) 107
HphI GGTGA 4 cut(s) 4, 76, 136, 451
Hpy166II GTNNAC 4 cut(s) 6, 119, 271, 442
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 2 cut(s) 70, 449
Hpy8I GTNNAC 4 cut(s) 6, 119, 271, 442
Hpy99I CGWCG 2 cut(s) 10, 134
HpyCH4III ACNGT 3 cut(s) 10, 30, 295
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 3 cut(s) 163, 311, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 103
HpyF3I CTNAG 5 cut(s) 33, 68, 135, 183, 210
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 3 cut(s) 173, 262, 332
LmnI GCTCC 2 cut(s) 102, 469
MaeI CTAG 2 cut(s) 255, 344
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 10, 373
MalI GATC 3 cut(s) 175, 264, 334
MboI GATC 3 cut(s) 173, 262, 332
MboII GAAGA 2 cut(s) 73, 238
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 2 cut(s) 99, 466
MluCI AATT 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 174
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 3 cut(s) 63, 285, 471
MvaI CCWGG 3 cut(s) 63, 285, 471
MwoI GCNNNNNNNGC 2 cut(s) 43, 103
NdeII GATC 3 cut(s) 173, 262, 332
NheI GCTAGC 1 cut(s) 343
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 3 cut(s) 27, 362, 465
NmuCI GTSAC 1 cut(s) 10
NspI RCATGY 1 cut(s) 325
PciI ACATGT 1 cut(s) 321
PfeI GAWTC 1 cut(s) 204
Ple19I CGATCG 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 139
PscI ACATGT 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 61, 283, 469
PspEI GGTNACC 1 cut(s) 10
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 3 cut(s) 61, 283, 469
PspN4I GGNNCC 3 cut(s) 27, 362, 465
PspPI GGNCC 3 cut(s) 139, 169, 361
PspPPI RGGWCCY 1 cut(s) 139
PsuI RGATCY 1 cut(s) 332
PvuI CGATCG 1 cut(s) 176
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
SacI GAGCTC 1 cut(s) 99
SalI GTCGAC 1 cut(s) 4
Sau3AI GATC 3 cut(s) 173, 262, 332
Sau96I GGNCC 3 cut(s) 139, 169, 361
ScrFI CCNGG 3 cut(s) 63, 285, 471
SduI GDGCHC 2 cut(s) 99, 466
SinI GGWCC 1 cut(s) 139
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 1 cut(s) 388
SsiI CCGC 3 cut(s) 193, 304, 467
SspMI CTAG 2 cut(s) 255, 344
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 3 cut(s) 61, 283, 469
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 3 cut(s) 10, 30, 295
TaiI ACGT 1 cut(s) 447
TaqI TCGA 2 cut(s) 5, 93
TaqII GACCGA 1 cut(s) 166
TasI AATT 1 cut(s) 388
TfiI GAWTC 1 cut(s) 204
TscAI CASTG 2 cut(s) 121, 256
TseFI GTSAC 1 cut(s) 10
Tsp45I GTSAC 1 cut(s) 10
TspRI CASTG 2 cut(s) 121, 256
VpaK11BI GGWCC 1 cut(s) 139
XapI RAATTY 1 cut(s) 388
XceI RCATGY 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 255, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.