Rorug05G0131600

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010659
Physical Location & Seq
Forward (+)
11749702 .. 11751584
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0131600.1

Sequence Viewer

Length: 744 bp
ATGTCAGCAGCGGCGGCCGCGGCAGCTGCAGCTGTGGTGGTGAGCCGTTCGAGCTCCAGCAACAGCAGCTCGAGCGCGGAGACCTACCACCACCTCCACCATCACCAATCGTTCATGGCACCGTCGCTGCCTGTGGCGCCTCAGCTCAGCCGTTACGAGTCGCAGAAGAGGAGGGACTGGATCACTTTTGGTCAGTATCTGAAGAACCATCGCCCGCCGCTGACTCTGTCACGGTGCAGCGGAGCCCACGTGCTGGAGTTTCTGAGGTACTTGGACCAGTTCGGGAAGACCAAGGTCCACGGGAGTGCCTGCCCTTTCTACGGCCACCCCCACCCGCCAGCGCCGTGTCCCTGCCCGCTGCGGCAGGCCTGGGGCAGCCTGGACGCGCTCATTGGGCGGCTGAGGGCAGCGTTCGAGGAGAACGGCGGGCTGCCAGAGACAAACCCTTTCGGCGCACGCGCCGTGAGGCTGTACCTGAGGGAAGTGAGGGACACGCAGGCCAAAGCTAGAGGGATTGCTTATGAGAAGAAGAAGCGAAAGAAGCCAACGCCACCACAAAGGACGACACAACTGCTGCAGCAGCATGAGGAGCAAGGGCAACATGGCAATTTTGTTTCTGGTTACGGTGGTGCTGGGGTTGGAGTTGGGGGTGGCTCAGCTCAGCCAAATGGGGTTATGCTCGGTGGAGCTTCGTCTACTGCTGCGAGAGGATCCATGATGCCGCTATCAGTGTTGAACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

26.57

Weight (kDa)

9.94

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALOG_dom PF04852 46 - 164 1.2e-64 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 118, 136
AccB7I CCANNNNNTGG 1 cut(s) 253
AccI GTMKAC 1 cut(s) 695
AccII CGCG 4 cut(s) 20, 77, 386, 459
AclWI GGATC 3 cut(s) 188, 705, 718
AcoI YGGCCR 2 cut(s) 15, 322
AcuI CTGAAG 1 cut(s) 221
AcvI CACGTG 1 cut(s) 250
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 2 cut(s) 269, 473
AfiI CCNNNNNNNGG 2 cut(s) 253, 320
AgsI TTSAA 1 cut(s) 736
AjnI CCWGG 2 cut(s) 368, 378
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 8 cut(s) 26, 32, 54, 69, 145, 506, 659, 689
AluI AGCT 8 cut(s) 26, 32, 54, 69, 145, 506, 659, 689
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 74, 431
AlwI GGATC 3 cut(s) 188, 705, 718
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 4 cut(s) 15, 322, 366, 498
AspLEI GCGC 6 cut(s) 77, 139, 343, 388, 455, 461
AspS9I GGNCC 2 cut(s) 274, 295
AsuHPI GGTGA 2 cut(s) 52, 95
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 2 cut(s) 274, 295
AxyI CCTNAGG 1 cut(s) 476
BamHI GGATCC 1 cut(s) 710
BanI GGYRCC 2 cut(s) 118, 136
BanII GRGCYC 2 cut(s) 56, 247
BbrPI CACGTG 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 56
BbvCI CCTCAGC 2 cut(s) 141, 401
BccI CCATC 2 cut(s) 108, 216
BceAI ACGGC 6 cut(s) 30, 135, 328, 337, 439, 446
BcgI CGANNNNNNTGC 2 cut(s) 553, 587
BciT130I CCWGG 2 cut(s) 370, 380
BcoDI GTCTC 2 cut(s) 74, 431
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 2 cut(s) 27, 575
BfoI RGCGCY 2 cut(s) 140, 344
BlpI GCTNAGC 3 cut(s) 146, 655, 660
Bme1390I CCNGG 2 cut(s) 370, 380
Bme18I GGWCC 2 cut(s) 274, 295
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 274, 295
BmiI GGNNCC 4 cut(s) 120, 138, 244, 712
BmrFI CCNGG 2 cut(s) 370, 380
BmsI GCATC 1 cut(s) 708
BoxI GACNNNNGTC 1 cut(s) 293
BpiI GAAGAC 1 cut(s) 293
BpmI CTGGAG 2 cut(s) 40, 275
Bpu10I CCTNAGC 2 cut(s) 141, 401
Bpu1102I GCTNAGC 3 cut(s) 146, 655, 660
BsaAI YACGTR 1 cut(s) 250
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 18, 291, 298, 369
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 320
Bse1I ACTGG 2 cut(s) 182, 277
Bse21I CCTNAGG 1 cut(s) 476
BseBI CCWGG 2 cut(s) 370, 380
BseDI CCNNGG 4 cut(s) 18, 291, 298, 369
BseLI CCNNNNNNNGG 2 cut(s) 253, 320
BseMII CTCAG 7 cut(s) 155, 160, 254, 392, 467, 669, 674
BseNI ACTGG 2 cut(s) 182, 277
BseRI GAGGAG 3 cut(s) 184, 431, 602
BseX3I CGGCCG 1 cut(s) 15
BseYI CCCAGC 1 cut(s) 632
BsgI GTGCAG 1 cut(s) 256
Bsh1236I CGCG 4 cut(s) 20, 77, 386, 459
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 4 cut(s) 17, 324, 368, 500
BshNI GGYRCC 2 cut(s) 118, 136
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 70
BslFI GGGAC 3 cut(s) 188, 333, 503
BslI CCNNNNNNNGG 2 cut(s) 253, 320
BsmAI GTCTC 2 cut(s) 74, 431
BsmFI GGGAC 3 cut(s) 188, 333, 503
BsnI GGCC 4 cut(s) 17, 324, 368, 500
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 2 cut(s) 56, 247
Bsp143I GATC 2 cut(s) 180, 710
Bsp1720I GCTNAGC 3 cut(s) 146, 655, 660
BspANI GGCC 4 cut(s) 17, 324, 368, 500
BspCNI CTCAG 7 cut(s) 154, 159, 255, 393, 468, 668, 673
BspFNI CGCG 4 cut(s) 20, 77, 386, 459
BspLI GGNNCC 4 cut(s) 120, 138, 244, 712
BspMAI CTGCAG 2 cut(s) 31, 579
BspPI GGATC 3 cut(s) 188, 705, 718
BspT107I GGYRCC 2 cut(s) 118, 136
BspTNI GGTCTC 1 cut(s) 74
BsrI ACTGG 2 cut(s) 182, 277
BssECI CCNNGG 4 cut(s) 18, 291, 298, 369
BssMI GATC 2 cut(s) 180, 710
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 2 cut(s) 370, 380
Bst4CI ACNGT 3 cut(s) 123, 234, 626
Bst6I CTCTTC 1 cut(s) 161
BstACI GRCGYC 1 cut(s) 137
BstBAI YACGTR 1 cut(s) 250
BstC8I GCNNGC 8 cut(s) 215, 310, 339, 356, 366, 428, 457, 498
BstDEI CTNAG 7 cut(s) 141, 146, 263, 401, 476, 655, 660
BstDSI CCRYGG 2 cut(s) 18, 298
BstFNI CGCG 4 cut(s) 20, 77, 386, 459
BstH2I RGCGCY 2 cut(s) 140, 344
BstHHI GCGC 6 cut(s) 77, 139, 343, 388, 455, 461
BstKTI GATC 2 cut(s) 183, 713
BstMAI GTCTC 2 cut(s) 74, 431
BstMBI GATC 2 cut(s) 180, 710
BstMCI CGRYCG 1 cut(s) 18
BstNI CCWGG 2 cut(s) 370, 380
BstPAI GACNNNNGTC 1 cut(s) 293
BstSCI CCNGG 2 cut(s) 368, 378
BstSFI CTRYAG 2 cut(s) 27, 575
BstUI CGCG 4 cut(s) 20, 77, 386, 459
BstV2I GAAGAC 1 cut(s) 293
BstX2I RGATCY 1 cut(s) 710
BstYI RGATCY 1 cut(s) 710
BstZI CGGCCG 1 cut(s) 15
Bsu36I CCTNAGG 1 cut(s) 476
BsuRI GGCC 4 cut(s) 17, 324, 368, 500
BtgI CCRYGG 2 cut(s) 18, 298
BtgZI GCGATG 1 cut(s) 194
BtsIMutI CAGTG 1 cut(s) 735
Cac8I GCNNGC 8 cut(s) 215, 310, 339, 356, 366, 428, 457, 498
CaiI CAGNNNCTG 1 cut(s) 199
CciNI GCGGCCGC 1 cut(s) 15
CfoI GCGC 6 cut(s) 77, 139, 343, 388, 455, 461
Cfr13I GGNCC 2 cut(s) 274, 295
Cfr42I CCGCGG 1 cut(s) 21
CseI GACGC 1 cut(s) 392
Csp6I GTAC 2 cut(s) 268, 472
CviAII CATG 4 cut(s) 115, 584, 602, 715
CviQI GTAC 2 cut(s) 268, 472
DdeI CTNAG 7 cut(s) 141, 146, 263, 401, 476, 655, 660
DinI GGCGCC 1 cut(s) 138
DpnI GATC 2 cut(s) 182, 712
DpnII GATC 2 cut(s) 180, 710
EaeI YGGCCR 2 cut(s) 15, 322
EagI CGGCCG 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Ecl136II GAGCTC 1 cut(s) 54
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 291
Eco147I AGGCCT 1 cut(s) 368
Eco24I GRGCYC 2 cut(s) 56, 247
Eco31I GGTCTC 1 cut(s) 74
Eco47I GGWCC 2 cut(s) 274, 295
Eco52I CGGCCG 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 221
Eco72I CACGTG 1 cut(s) 250
Eco81I CCTNAGG 1 cut(s) 476
Eco88I CYCGRG 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 54
EcoRII CCWGG 2 cut(s) 368, 378
EcoT14I CCWWGG 1 cut(s) 291
EcoT38I GRGCYC 2 cut(s) 56, 247
EgeI GGCGCC 1 cut(s) 138
EheI GGCGCC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 4 cut(s) 118, 587, 605, 718
FaiI YATR 6 cut(s) 116, 522, 585, 603, 677, 716
FaqI GGGAC 3 cut(s) 188, 333, 503
FatI CATG 4 cut(s) 114, 583, 601, 714
FauI CCCGC 4 cut(s) 222, 342, 363, 419
FblI GTMKAC 1 cut(s) 695
FriOI GRGCYC 2 cut(s) 56, 247
FspBI CTAG 1 cut(s) 507
GlaI GCGC 6 cut(s) 76, 138, 342, 387, 454, 460
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 2 cut(s) 40, 275
HaeII RGCGCY 2 cut(s) 140, 344
HaeIII GGCC 4 cut(s) 17, 324, 368, 500
HgaI GACGC 1 cut(s) 392
HhaI GCGC 6 cut(s) 77, 139, 343, 388, 455, 461
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 4 cut(s) 118, 587, 605, 718
Hin6I GCGC 6 cut(s) 75, 137, 341, 386, 453, 459
HinP1I GCGC 6 cut(s) 75, 137, 341, 386, 453, 459
HinfI GANTC 2 cut(s) 158, 223
HphI GGTGA 2 cut(s) 52, 95
Hpy166II GTNNAC 2 cut(s) 298, 696
Hpy188I TCNGA 2 cut(s) 201, 264
Hpy188III TCNNGA 1 cut(s) 283
Hpy8I GTNNAC 2 cut(s) 298, 696
Hpy99I CGWCG 1 cut(s) 127
HpyCH4III ACNGT 3 cut(s) 123, 234, 626
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 3 cut(s) 29, 237, 577
HpyF3I CTNAG 7 cut(s) 141, 146, 263, 401, 476, 655, 660
HpySE526I ACGT 1 cut(s) 249
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 4 cut(s) 118, 587, 605, 718
HspAI GCGC 6 cut(s) 75, 137, 341, 386, 453, 459
KasI GGCGCC 1 cut(s) 136
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 2 cut(s) 180, 710
LmnI GCTCC 4 cut(s) 59, 242, 589, 686
LweI GCATC 1 cut(s) 708
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 3 cut(s) 152, 228, 620
MalI GATC 2 cut(s) 182, 712
MboI GATC 2 cut(s) 180, 710
MboII GAAGA 5 cut(s) 178, 214, 298, 538, 541
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 2 cut(s) 56, 247
MluCI AATT 1 cut(s) 607
Mly113I GGCGCC 1 cut(s) 137
MlyI GAGTC 2 cut(s) 167, 217
MmeI TCCRAC 1 cut(s) 619
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 7 cut(s) 11, 20, 26, 32, 220, 240, 358
MspR9I CCNGG 2 cut(s) 370, 380
MvaI CCWGG 2 cut(s) 370, 380
MvnI CGCG 4 cut(s) 20, 77, 386, 459
NarI GGCGCC 1 cut(s) 137
NdeII GATC 2 cut(s) 180, 710
NlaIII CATG 4 cut(s) 118, 587, 605, 718
NlaIV GGNNCC 4 cut(s) 120, 138, 244, 712
NmuCI GTSAC 1 cut(s) 228
NotI GCGGCCGC 1 cut(s) 15
OliI CACNNNNGTG 1 cut(s) 303
PaeR7I CTCGAG 1 cut(s) 70
PceI AGGCCT 1 cut(s) 368
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 253
PleI GAGTC 2 cut(s) 166, 217
PluTI GGCGCC 1 cut(s) 140
PmaCI CACGTG 1 cut(s) 250
PmlI CACGTG 1 cut(s) 250
PpsI GAGTC 2 cut(s) 166, 217
Ppu21I YACGTR 1 cut(s) 250
PshAI GACNNNNGTC 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 368, 378
PspCI CACGTG 1 cut(s) 250
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 2 cut(s) 368, 378
PspN4I GGNNCC 4 cut(s) 120, 138, 244, 712
PspPI GGNCC 2 cut(s) 274, 295
PspXI VCTCGAGB 1 cut(s) 70
PstI CTGCAG 2 cut(s) 31, 579
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 710
PsyI GACNNNGTC 1 cut(s) 226
PvuII CAGCTG 2 cut(s) 26, 32
RsaI GTAC 2 cut(s) 269, 473
RsaNI GTAC 2 cut(s) 268, 472
RseI CAYNNNNRTG 1 cut(s) 303
SacI GAGCTC 1 cut(s) 56
SacII CCGCGG 1 cut(s) 21
Sau3AI GATC 2 cut(s) 180, 710
Sau96I GGNCC 2 cut(s) 274, 295
SchI GAGTC 2 cut(s) 167, 217
ScrFI CCNGG 2 cut(s) 370, 380
SduI GDGCHC 2 cut(s) 56, 247
SfaNI GCATC 1 cut(s) 708
SfcI CTRYAG 2 cut(s) 27, 575
SfoI GGCGCC 1 cut(s) 138
Sfr274I CTCGAG 1 cut(s) 70
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 2 cut(s) 274, 295
SlaI CTCGAG 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
Sse9I AATT 1 cut(s) 607
SseBI AGGCCT 1 cut(s) 368
SspDI GGCGCC 1 cut(s) 136
SspMI CTAG 1 cut(s) 507
SstI GAGCTC 1 cut(s) 56
StuI AGGCCT 1 cut(s) 368
StyD4I CCNGG 2 cut(s) 368, 378
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 3 cut(s) 123, 234, 626
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 50, 71, 414
TasI AATT 1 cut(s) 607
TauI GCSGC 8 cut(s) 14, 17, 20, 23, 220, 364, 400, 724
TscAI CASTG 1 cut(s) 735
TseFI GTSAC 1 cut(s) 228
Tsp45I GTSAC 1 cut(s) 228
TspDTI ATGAA 1 cut(s) 103
TspRI CASTG 1 cut(s) 735
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 2 cut(s) 274, 295
XhoI CTCGAG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 695
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.