FvH4_3g20621

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0017928
Physical Location & Seq
Forward (+)
13674752 .. 13676756
2005 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20621.t1

Sequence Viewer

Length: 999 bp
ATGGTCCAAATTTTGTCAAAACAACCAAGCAAATCACTGTGGTTTAAGGTACTCCAGGATGCATTTACATGGACTAAAGCTTCTAAGTGGAGAAAAGTGGCCAAACGCAAAAGCATAGTTGAGCAACTTCAGTGTCATTTGATCATCCAAAAGAAGAAGAGACAATATATTATAAGGCAGGAACGTGACGACATCGGTCAGCTCCTCCAAATTGGGCAGTTAGACCAAGCTCGAGCTAGGGCAGTTTTTCACTTTTCATGGAACACATTTGTTCGGTACTTCAGTGCTTATATTACAGAATACAGTACCTTGCAAGAACTGCCAAGTGATGTTGAAGCGATAGCAAGTTTGATTTATGCTGCCTCAAGATGTGGTGAACTGCCAGAGCTACAACGAATGCGTTGCCTGTTCAAAGAGAAATATGGATCGGAATTTGACATTACTAGCGTTGAACTACGGCATGGTAATCTTGTGAATCCTCAGTTGAAAGAGAAGCTACGTATAACTCGGATTCCAGATGAGGTGAAACAAGAGCTGATAGGAGATACAGTTACCAAGTGCAACATCCGCCTAGCCCTCCAAGATGTCCAACACACGACATTCCAGAACTATAGCTTCCCAGACAAATGTTTCAAGGATGAGCAGAAAGATGAGGGAAATCGAATACAAGTTCCAAAATCGGACATGGGGACGAAACCTGTTGGGATATTTTGTTCTTTGTCAAAAGGTAAACTTGTAGTGGCCACTCCATTCCCTTCTGATATTGGCTCTGGTTTAGGTAGCTCAAACCCTATGACTAACTTAGCCAGAAAAGGCTCAATGGGGAAAAGATATGGTTCCAAGATGGTGAGGAGTTCAGTTGATGAAGTTAAAACGCCAAATGGAAGCTCTCCTTGCCATGTCCACCCCAAGCTTCCGGAATATGATGACGTGGTGGCCAAACTTCTCCATCTTAAGGCCGAACACACAAAGAGGCGTAAAACCTACACATTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

333

Amino Acids

38.06

Weight (kDa)

9.6

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 37 - 182 1.2e-21 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15490
fragaria_vesca FvH4_3g20621
malus_domestica MD03G1223500.v1.1
prunus_persica Prupe.4G186000_v2.0.a1 Prupe.4G186000_v2.0.a1
rosa_laevigata RLG00000033564
rosa_multiflora Rmu_sc0002460.1_g000083
rosa_roxburghii Rroxscaffold_1G00045670
rosa_samantha Rh5BG240200 Rh5CG270600 Rh5DG248400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 916
AciI CCGC 1 cut(s) 568
AclWI GGATC 1 cut(s) 433
AcoI YGGCCR 3 cut(s) 99, 741, 936
AcsI RAATTY 2 cut(s) 9, 431
AcuI CTGAAG 2 cut(s) 113, 265
AfaI GTAC 3 cut(s) 51, 278, 307
AfiI CCNNNNNNNGG 1 cut(s) 955
AflII CTTAAG 1 cut(s) 953
AgsI TTSAA 5 cut(s) 335, 412, 452, 487, 634
AjiI CACGTC 1 cut(s) 931
AjnI CCWGG 1 cut(s) 54
AloI GAACNNNNNNTCC 2 cut(s) 697, 729
Alw26I GTCTC 1 cut(s) 154
AlwI GGATC 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 231
Aor13HI TCCGGA 1 cut(s) 916
AoxI GGCC 4 cut(s) 99, 741, 936, 957
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 2 cut(s) 9, 431
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 3 cut(s) 386, 535, 859
AvaI CYCGRG 1 cut(s) 231
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 268, 301
BalI TGGCCA 3 cut(s) 101, 743, 938
BbvI GCAGC 1 cut(s) 346
BccI CCATC 2 cut(s) 838, 957
BceAI ACGGC 1 cut(s) 473
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BciT130I CCWGG 1 cut(s) 56
BclI TGATCA 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 3 cut(s) 237, 444, 572
BfmI CTRYAG 1 cut(s) 610
BfrI CTTAAG 1 cut(s) 953
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 56
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 231
BmgBI CACGTC 1 cut(s) 931
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 838
BmrFI CCNGG 1 cut(s) 56
BmsI GCATC 1 cut(s) 49
BoxI GACNNNNGTC 1 cut(s) 195
BpmI CTGGAG 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 349
BsaAI YACGTR 1 cut(s) 500
BsaWI WCCGGW 1 cut(s) 916
BsaXI ACNNNNNCTCC 2 cut(s) 534, 564
Bsc4I CCNNNNNNNGG 1 cut(s) 955
BseAI TCCGGA 1 cut(s) 916
BseBI CCWGG 1 cut(s) 56
BseGI GGATG 4 cut(s) 64, 144, 564, 643
BseLI CCNNNNNNNGG 1 cut(s) 955
BseMII CTCAG 1 cut(s) 494
BseRI GAGGAG 2 cut(s) 194, 865
BseXI GCAGC 1 cut(s) 346
BshFI GGCC 4 cut(s) 101, 743, 938, 959
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 1 cut(s) 917
BslFI GGGAC 1 cut(s) 703
BslI CCNNNNNNNGG 1 cut(s) 955
BsmAI GTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 703
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 4 cut(s) 101, 743, 938, 959
BsoBI CYCGRG 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 916
Bsp143I GATC 2 cut(s) 141, 425
BspACI CCGC 1 cut(s) 568
BspANI GGCC 4 cut(s) 101, 743, 938, 959
BspCNI CTCAG 1 cut(s) 493
BspEI TCCGGA 1 cut(s) 916
BspLI GGNNCC 1 cut(s) 838
BspPI GGATC 1 cut(s) 433
BspTI CTTAAG 1 cut(s) 953
BssMI GATC 2 cut(s) 141, 425
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 3 cut(s) 39, 305, 550
Bst6I CTCTTC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 953
BstAPI GCANNNNNTGC 1 cut(s) 319
BstBAI YACGTR 1 cut(s) 500
BstDEI CTNAG 3 cut(s) 84, 480, 802
BstF5I GGATG 4 cut(s) 64, 144, 564, 643
BstKTI GATC 2 cut(s) 144, 428
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 2 cut(s) 141, 425
BstMWI GCNNNNNNNGC 3 cut(s) 319, 567, 894
BstNI CCWGG 1 cut(s) 56
BstPAI GACNNNNGTC 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 54
BstSFI CTRYAG 1 cut(s) 610
BstSNI TACGTA 1 cut(s) 500
BstV1I GCAGC 1 cut(s) 346
BsuRI GGCC 4 cut(s) 101, 743, 938, 959
BtrI CACGTC 1 cut(s) 931
BtsCI GGATG 4 cut(s) 64, 144, 564, 643
BtsIMutI CAGTG 3 cut(s) 35, 137, 289
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 3 cut(s) 50, 277, 306
CviAII CATG 5 cut(s) 69, 258, 461, 685, 899
CviQI GTAC 3 cut(s) 50, 277, 306
DdeI CTNAG 3 cut(s) 84, 480, 802
DpnI GATC 2 cut(s) 143, 427
DpnII GATC 2 cut(s) 141, 425
EaeI YGGCCR 3 cut(s) 99, 741, 936
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
EciI GGCGGA 1 cut(s) 557
Eco105I TACGTA 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 2 cut(s) 113, 265
Eco88I CYCGRG 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 64
FaeI CATG 5 cut(s) 72, 261, 464, 688, 902
FalI AAGNNNNNCTT 4 cut(s) 717, 749, 877, 909
FaqI GGGAC 1 cut(s) 703
FatI CATG 5 cut(s) 68, 257, 460, 684, 898
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 4 cut(s) 71, 131, 551, 650
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 3 cut(s) 237, 444, 572
GluI GCNGC 1 cut(s) 360
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 4 cut(s) 101, 743, 938, 959
HapII CCGG 1 cut(s) 917
Hin1II CATG 5 cut(s) 72, 261, 464, 688, 902
HindIII AAGCTT 2 cut(s) 78, 911
HinfI GANTC 2 cut(s) 475, 511
HpaII CCGG 1 cut(s) 917
HphI GGTGA 3 cut(s) 386, 535, 859
Hpy166II GTNNAC 3 cut(s) 377, 731, 904
Hpy188I TCNGA 5 cut(s) 430, 510, 682, 760, 998
Hpy188III TCNNGA 4 cut(s) 366, 515, 604, 917
Hpy8I GTNNAC 3 cut(s) 377, 731, 904
HpyAV CCTTC 1 cut(s) 765
HpyCH4III ACNGT 3 cut(s) 39, 305, 550
HpyCH4IV ACGT 3 cut(s) 184, 499, 930
HpyCH4V TGCA 3 cut(s) 62, 313, 561
HpyF10VI GCNNNNNNNGC 3 cut(s) 319, 567, 894
HpyF3I CTNAG 3 cut(s) 84, 480, 802
HpySE526I ACGT 3 cut(s) 184, 499, 930
Hsp92II CATG 5 cut(s) 72, 261, 464, 688, 902
Kpn2I TCCGGA 1 cut(s) 916
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 141, 425
LmnI GCTCC 1 cut(s) 207
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 1 cut(s) 49
MaeI CTAG 3 cut(s) 237, 444, 572
MaeII ACGT 3 cut(s) 184, 499, 930
MaeIII GTNAC 2 cut(s) 185, 550
MalI GATC 2 cut(s) 143, 427
MboI GATC 2 cut(s) 141, 425
MboII GAAGA 2 cut(s) 166, 169
MlsI TGGCCA 3 cut(s) 101, 743, 938
MluCI AATT 3 cut(s) 9, 210, 431
MluNI TGGCCA 3 cut(s) 101, 743, 938
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 8 cut(s) 215, 373, 489, 514, 587, 646, 843, 966
Mox20I TGGCCA 3 cut(s) 101, 743, 938
Mph1103I ATGCAT 1 cut(s) 64
MroI TCCGGA 1 cut(s) 916
MscI TGGCCA 3 cut(s) 101, 743, 938
MseI TTAA 3 cut(s) 45, 870, 954
MslI CAYNNNNRTG 1 cut(s) 67
Msp20I TGGCCA 3 cut(s) 101, 743, 938
MspCI CTTAAG 1 cut(s) 953
MspI CCGG 1 cut(s) 917
MspR9I CCNGG 1 cut(s) 56
Mva1269I GAATGC 1 cut(s) 402
MvaI CCWGG 1 cut(s) 56
MwoI GCNNNNNNNGC 3 cut(s) 319, 567, 894
NdeII GATC 2 cut(s) 141, 425
NlaIII CATG 5 cut(s) 72, 261, 464, 688, 902
NlaIV GGNNCC 1 cut(s) 838
NmuCI GTSAC 1 cut(s) 185
NsiI ATGCAT 1 cut(s) 64
PaeR7I CTCGAG 1 cut(s) 231
PctI GAATGC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 475, 511
PfoI TCCNGGA 1 cut(s) 54
PkrI GCNGC 1 cut(s) 361
Ppu21I YACGTR 1 cut(s) 500
PshAI GACNNNNGTC 1 cut(s) 195
PsiI TTATAA 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 54
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 838
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 231
RsaI GTAC 3 cut(s) 51, 278, 307
RsaNI GTAC 3 cut(s) 50, 277, 306
RseI CAYNNNNRTG 1 cut(s) 67
SaqAI TTAA 3 cut(s) 45, 870, 954
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 2 cut(s) 141, 425
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 56
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 610
Sfr274I CTCGAG 1 cut(s) 231
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 67
SmlI CTYRAG 3 cut(s) 231, 364, 953
SmoI CTYRAG 3 cut(s) 231, 364, 953
SnaBI TACGTA 1 cut(s) 500
Sse9I AATT 3 cut(s) 9, 210, 431
SsiI CCGC 1 cut(s) 568
SspMI CTAG 3 cut(s) 237, 444, 572
StyD4I CCNGG 1 cut(s) 54
TaaI ACNGT 3 cut(s) 39, 305, 550
TaiI ACGT 3 cut(s) 187, 502, 933
TaqI TCGA 2 cut(s) 232, 661
TaqII GACCGA 1 cut(s) 185
TasI AATT 3 cut(s) 9, 210, 431
TfiI GAWTC 2 cut(s) 475, 511
Tru1I TTAA 3 cut(s) 45, 870, 954
Tru9I TTAA 3 cut(s) 45, 870, 954
TscAI CASTG 3 cut(s) 42, 137, 289
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 1 cut(s) 359
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 2 cut(s) 246, 879
TspRI CASTG 3 cut(s) 42, 137, 289
Vha464I CTTAAG 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 9, 431
XhoI CTCGAG 1 cut(s) 231
XspI CTAG 3 cut(s) 237, 444, 572
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.