Rroxscaffold_1G00045670

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017928
Physical Location & Seq
Reverse (-)
64823779 .. 64825866
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00045670.1

Sequence Viewer

Length: 1086 bp
ATGGTCCAAATTCTGTCAAGGAAATTATTAACAGAACCAAGCAAATCACTGTGGTCTAAGGCACTCCAAGAAGCATTTATATGGACTAAAACTTTAAAGTGGAGAAAAGTGGCCAAATGCAAAAGCATAGTTGAGCGACTTCAGTGTCATTTGATCATCCAAAAGAATAGGAGGCAAGCTATCATAAGGCAAGAACGTACCGATATCGGTCAGCTCCTCCAAATTGGGCAGCTAGAACAAGCTCGAGCTAGGATCGAGAAACTGTACAAGGGCCAGTGCCTATTGGATGCATATGATCAGATCGACTCTTTCTGCAACTGCATTACCACTAATATTGCCTATATATACAAACACAAACACAGTACCTTGCAAGACTTGCCTAGTGATGTTGGCGAAGCGATCGCAAGTTTGATTTATGCTGCCTCAAGATGTGAAGAATTGCCAGAGCTACACCGAAAACGTTGCCTGTTCAAAGAGAAATATGGATCAGAATTTGACATTACCAGCGTTAAACTACGGCATGGGAATCTTGTGAATCCTCAGTTGAAAGAGAAGCTAGGCATAACTCGGATTCCAGATGAGGTGAAACAAGAGCTGATAGGAGATATAGTTACCCAGGGCAATATCCGTCTGGCCCTCCAGGATGTCCAAGACACTATACACCAGAACTATAGCTTTCCAGACAAAAGTTTCAAGTACGAGCAGAAAGCTGATGGGATTCGAATACGAGTTCCAAAATGGGAGATGGGGAGGAAACCTGTTGGGATATTTTGTTCTTTGTCAAAAGGTAAACTTTCGGTGGCCACTGCATTCCCTTCTTATATTGGCTCAAATAATGGTTCAGGTAGCTCAACCCCTATGACTAATTTAGCCAGAAAAGGCTCAAATGGGAAAAGAGATGGTTCCAAGATGGTGATGAATCACCATTCTTCCAATACTTCAGTTGATGAAGTCAAAATGTCAAACGGAAGCTCTCCTTGCTCCAGCCATGTCCACCCCAAGCTTCCGGAATATGATGATGTGGTGGCCAAACTTCGCCGTCTCAAGGCAGAACATAGTAGGCGTAAGACCTTTGCATTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

361

Amino Acids

41.06

Weight (kDa)

9.64

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 44 - 209 1.1e-36 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15490
fragaria_vesca FvH4_3g20621
malus_domestica MD03G1223500.v1.1
prunus_persica Prupe.4G186000_v2.0.a1 Prupe.4G186000_v2.0.a1
rosa_laevigata RLG00000033564
rosa_multiflora Rmu_sc0002460.1_g000083
rosa_roxburghii Rroxscaffold_1G00045670
rosa_samantha Rh5BG240200 Rh5CG270600 Rh5DG248400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 144
AccIII TCCGGA 1 cut(s) 1006
AclI AACGTT 1 cut(s) 460
AclWI GGATC 2 cut(s) 260, 493
AcoI YGGCCR 3 cut(s) 111, 801, 1026
AcsI RAATTY 2 cut(s) 9, 491
AcuI CTGAAG 2 cut(s) 125, 924
AfaI GTAC 4 cut(s) 199, 266, 364, 698
AfiI CCNNNNNNNGG 1 cut(s) 1045
AgsI TTSAA 3 cut(s) 472, 547, 694
AjnI CCWGG 2 cut(s) 615, 639
AloI GAACNNNNNNTCC 2 cut(s) 757, 789
Alw26I GTCTC 1 cut(s) 1046
AlwI GGATC 2 cut(s) 260, 493
Ama87I CYCGRG 1 cut(s) 243
Aor13HI TCCGGA 1 cut(s) 1006
AoxI GGCC 5 cut(s) 111, 271, 633, 801, 1026
ApeKI GCWGC 2 cut(s) 229, 419
ApoI RAATTY 2 cut(s) 9, 491
AsiSI GCGATCGC 1 cut(s) 402
AspS9I GGNCC 3 cut(s) 4, 271, 634
AsuHPI GGTGA 3 cut(s) 595, 914, 925
AsuII TTCGAA 1 cut(s) 721
AvaI CYCGRG 1 cut(s) 243
AvaII GGWCC 1 cut(s) 4
BalI TGGCCA 3 cut(s) 113, 803, 1028
BbvI GCAGC 2 cut(s) 241, 406
BccI CCATC 4 cut(s) 707, 739, 893, 904
BceAI ACGGC 2 cut(s) 533, 1023
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciT130I CCWGG 2 cut(s) 617, 641
BclI TGATCA 2 cut(s) 153, 295
BcoDI GTCTC 1 cut(s) 1046
BfaI CTAG 4 cut(s) 233, 249, 381, 557
BfmI CTRYAG 1 cut(s) 670
BisI GCNGC 2 cut(s) 230, 420
BlsI GCNGC 2 cut(s) 231, 421
Bme1390I CCNGG 2 cut(s) 617, 641
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 243
BmgT120I GGNCC 3 cut(s) 4, 271, 634
BmiI GGNNCC 1 cut(s) 904
BmrFI CCNGG 2 cut(s) 617, 641
BmsI GCATC 1 cut(s) 277
BpmI CTGGAG 2 cut(s) 623, 967
Bpu14I TTCGAA 1 cut(s) 721
BpuEI CTTGAG 2 cut(s) 409, 1028
BsaJI CCNNGG 2 cut(s) 615, 616
BsaWI WCCGGW 1 cut(s) 1006
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 1045
Bse1I ACTGG 1 cut(s) 274
BseAI TCCGGA 1 cut(s) 1006
BseBI CCWGG 2 cut(s) 617, 641
BseDI CCNNGG 2 cut(s) 615, 616
BseGI GGATG 3 cut(s) 156, 292, 649
BseLI CCNNNNNNNGG 1 cut(s) 1045
BseMII CTCAG 1 cut(s) 554
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 2 cut(s) 241, 406
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 5 cut(s) 113, 273, 635, 803, 1028
BsiEI CGRYCG 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 1 cut(s) 1007
BslI CCNNNNNNNGG 1 cut(s) 1045
BsmAI GTCTC 1 cut(s) 1046
BsmBI CGTCTC 1 cut(s) 1046
BsmI GAATGC 1 cut(s) 809
BsnI GGCC 5 cut(s) 113, 273, 635, 803, 1028
BsoBI CYCGRG 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 721
Bsp13I TCCGGA 1 cut(s) 1006
Bsp1407I TGTACA 1 cut(s) 264
Bsp143I GATC 6 cut(s) 153, 252, 295, 300, 399, 485
BspANI GGCC 5 cut(s) 113, 273, 635, 803, 1028
BspCNI CTCAG 1 cut(s) 553
BspEI TCCGGA 1 cut(s) 1006
BspLI GGNNCC 1 cut(s) 904
BspPI GGATC 2 cut(s) 260, 493
BspT104I TTCGAA 1 cut(s) 721
BsrGI TGTACA 1 cut(s) 264
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 2 cut(s) 615, 616
BssMI GATC 6 cut(s) 153, 252, 295, 300, 399, 485
Bst2UI CCWGG 2 cut(s) 617, 641
Bst4CI ACNGT 3 cut(s) 51, 264, 362
BstAPI GCANNNNNTGC 1 cut(s) 376
BstAUI TGTACA 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 721
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 2 cut(s) 57, 540
BstF5I GGATG 3 cut(s) 156, 292, 649
BstKTI GATC 6 cut(s) 156, 255, 298, 303, 402, 488
BstMAI GTCTC 1 cut(s) 1046
BstMBI GATC 6 cut(s) 153, 252, 295, 300, 399, 485
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 2 cut(s) 376, 978
BstNI CCWGG 2 cut(s) 617, 641
BstSCI CCNGG 2 cut(s) 615, 639
BstSFI CTRYAG 1 cut(s) 670
BstV1I GCAGC 2 cut(s) 241, 406
BsuRI GGCC 5 cut(s) 113, 273, 635, 803, 1028
BtsCI GGATG 3 cut(s) 156, 292, 649
BtsI GCAGTG 1 cut(s) 804
BtsIMutI CAGTG 4 cut(s) 47, 149, 281, 804
Cac8I GCNNGC 1 cut(s) 177
Cfr13I GGNCC 3 cut(s) 4, 271, 634
Csp6I GTAC 4 cut(s) 198, 265, 363, 697
CspCI CAANNNNNGTGG 2 cut(s) 316, 351
CviAII CATG 2 cut(s) 521, 989
CviQI GTAC 4 cut(s) 198, 265, 363, 697
DdeI CTNAG 2 cut(s) 57, 540
DpnI GATC 6 cut(s) 155, 254, 297, 302, 401, 487
DpnII GATC 6 cut(s) 153, 252, 295, 300, 399, 485
DraI TTTAAA 1 cut(s) 96
DrdI GACNNNNNNGTC 1 cut(s) 144
DseDI GACNNNNNNGTC 1 cut(s) 144
EaeI YGGCCR 3 cut(s) 111, 801, 1026
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 2 cut(s) 125, 924
Eco88I CYCGRG 1 cut(s) 243
EcoRII CCWGG 2 cut(s) 615, 639
EcoRV GATATC 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 292
Esp3I CGTCTC 1 cut(s) 1046
FaeI CATG 2 cut(s) 524, 992
FalI AAGNNNNNCTT 4 cut(s) 777, 809, 961, 993
FatI CATG 2 cut(s) 520, 988
FauNDI CATATG 1 cut(s) 292
FbaI TGATCA 2 cut(s) 153, 295
Fnu4HI GCNGC 2 cut(s) 230, 420
FokI GGATG 3 cut(s) 143, 299, 656
Fsp4HI GCNGC 2 cut(s) 230, 420
FspBI CTAG 4 cut(s) 233, 249, 381, 557
GluI GCNGC 2 cut(s) 230, 420
GsuI CTGGAG 2 cut(s) 623, 967
HaeIII GGCC 5 cut(s) 113, 273, 635, 803, 1028
HapII CCGG 1 cut(s) 1007
Hin1II CATG 2 cut(s) 524, 992
HindIII AAGCTT 1 cut(s) 1001
HinfI GANTC 6 cut(s) 305, 526, 535, 571, 718, 919
HpaII CCGG 1 cut(s) 1007
HphI GGTGA 3 cut(s) 595, 914, 925
Hpy166II GTNNAC 2 cut(s) 791, 994
Hpy188I TCNGA 4 cut(s) 300, 490, 570, 1085
Hpy188III TCNNGA 5 cut(s) 256, 426, 575, 680, 1007
Hpy8I GTNNAC 2 cut(s) 791, 994
HpyAV CCTTC 1 cut(s) 825
HpyCH4III ACNGT 3 cut(s) 51, 264, 362
HpyCH4IV ACGT 2 cut(s) 196, 460
HpyCH4V TGCA 7 cut(s) 120, 290, 315, 321, 370, 809, 1076
HpyF10VI GCNNNNNNNGC 2 cut(s) 376, 978
HpyF3I CTNAG 2 cut(s) 57, 540
HpySE526I ACGT 2 cut(s) 196, 460
Hsp92II CATG 2 cut(s) 524, 992
Kpn2I TCCGGA 1 cut(s) 1006
Ksp22I TGATCA 2 cut(s) 153, 295
Kzo9I GATC 6 cut(s) 153, 252, 295, 300, 399, 485
LmnI GCTCC 2 cut(s) 219, 986
Lsp1109I GCAGC 2 cut(s) 241, 406
LweI GCATC 1 cut(s) 277
MaeI CTAG 4 cut(s) 233, 249, 381, 557
MaeII ACGT 2 cut(s) 196, 460
MaeIII GTNAC 1 cut(s) 610
MalI GATC 6 cut(s) 155, 254, 297, 302, 401, 487
MboI GATC 6 cut(s) 153, 252, 295, 300, 399, 485
MboII GAAGA 2 cut(s) 446, 921
MlsI TGGCCA 3 cut(s) 113, 803, 1028
MluCI AATT 6 cut(s) 9, 23, 222, 437, 491, 865
MluNI TGGCCA 3 cut(s) 113, 803, 1028
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 7 cut(s) 165, 227, 433, 549, 574, 647, 744
Mox20I TGGCCA 3 cut(s) 113, 803, 1028
Mph1103I ATGCAT 1 cut(s) 292
MroI TCCGGA 1 cut(s) 1006
MscI TGGCCA 3 cut(s) 113, 803, 1028
MseI TTAA 3 cut(s) 29, 95, 510
MslI CAYNNNNRTG 1 cut(s) 79
Msp20I TGGCCA 3 cut(s) 113, 803, 1028
MspI CCGG 1 cut(s) 1007
MspR9I CCNGG 2 cut(s) 617, 641
Mva1269I GAATGC 1 cut(s) 809
MvaI CCWGG 2 cut(s) 617, 641
MwoI GCNNNNNNNGC 2 cut(s) 376, 978
NdeI CATATG 1 cut(s) 292
NdeII GATC 6 cut(s) 153, 252, 295, 300, 399, 485
NlaIII CATG 2 cut(s) 524, 992
NlaIV GGNNCC 1 cut(s) 904
NsiI ATGCAT 1 cut(s) 292
NspV TTCGAA 1 cut(s) 721
PaeR7I CTCGAG 1 cut(s) 243
PasI CCCWGGG 1 cut(s) 616
PctI GAATGC 1 cut(s) 809
PfeI GAWTC 5 cut(s) 526, 535, 571, 718, 919
PfoI TCCNGGA 1 cut(s) 639
PkrI GCNGC 2 cut(s) 231, 421
Ple19I CGATCG 1 cut(s) 402
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
Psp1406I AACGTT 1 cut(s) 460
Psp6I CCWGG 2 cut(s) 615, 639
PspGI CCWGG 2 cut(s) 615, 639
PspN4I GGNNCC 1 cut(s) 904
PspPI GGNCC 3 cut(s) 4, 271, 634
PspXI VCTCGAGB 1 cut(s) 243
PvuI CGATCG 1 cut(s) 402
RgaI GCGATCGC 1 cut(s) 402
RsaI GTAC 4 cut(s) 199, 266, 364, 698
RsaNI GTAC 4 cut(s) 198, 265, 363, 697
RseI CAYNNNNRTG 1 cut(s) 79
SaqAI TTAA 3 cut(s) 29, 95, 510
SatI GCNGC 2 cut(s) 230, 420
Sau3AI GATC 6 cut(s) 153, 252, 295, 300, 399, 485
Sau96I GGNCC 3 cut(s) 4, 271, 634
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 2 cut(s) 617, 641
SfaAI GCGATCGC 1 cut(s) 402
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 1 cut(s) 670
Sfr274I CTCGAG 1 cut(s) 243
SfuI TTCGAA 1 cut(s) 721
SgfI GCGATCGC 1 cut(s) 402
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 79
SmlI CTYRAG 3 cut(s) 243, 424, 1043
SmoI CTYRAG 3 cut(s) 243, 424, 1043
Sse9I AATT 6 cut(s) 9, 23, 222, 437, 491, 865
SspI AATATT 1 cut(s) 334
SspMI CTAG 4 cut(s) 233, 249, 381, 557
StyD4I CCNGG 2 cut(s) 615, 639
TaaI ACNGT 3 cut(s) 51, 264, 362
TaiI ACGT 2 cut(s) 199, 463
TaqI TCGA 4 cut(s) 244, 255, 303, 721
TaqII GACCGA 1 cut(s) 197
TasI AATT 6 cut(s) 9, 23, 222, 437, 491, 865
TatI WGTACW 1 cut(s) 264
TfiI GAWTC 5 cut(s) 526, 535, 571, 718, 919
Tru1I TTAA 3 cut(s) 29, 95, 510
Tru9I TTAA 3 cut(s) 29, 95, 510
TscAI CASTG 4 cut(s) 54, 149, 281, 811
TseI GCWGC 2 cut(s) 229, 419
TspDTI ATGAA 2 cut(s) 932, 963
TspGWI ACGGA 2 cut(s) 617, 981
TspRI CASTG 4 cut(s) 54, 149, 281, 811
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 9, 491
XhoI CTCGAG 1 cut(s) 243
XspI CTAG 4 cut(s) 233, 249, 381, 557
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.