RLG00000033564

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017928
Physical Location & Seq
Forward (+)
29005323 .. 29007409
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033564

Sequence Viewer

Length: 756 bp
ATGGTCCAAATTCTGTCAAGGAAATTATTAACAGAACCAAGCAAATCACTGTGGTCTAAGGCACTCCAAGAAGCATTTATATGGACTAAAACTTCAAAGTGGAGAAAAGTGGCCAAATGCAAGCCCATAATGGGTCGAGTTTGCGGGGAAACGGGAGGCAGGGCTGAGGCACGGCCCTTCATTCTCCATCGACTCTTTCTGCAATTGCATTACCACTATTCCACTAACATTGCCTATATATACAAACACAAACACAGGACCTTGCAAGACTTGCCGAGTGATGTTGGCGAAGCGATCGCAAGTTTGATTTATGCTGCCTCAAGATGTGGAGAATTGCCAGAGCTACACCGAATACGTTGCCTGTTCAAAGAGAAATATGGATCAGAATTTGACATTACCAGCGTTAAACTACGGCATGGGAATCTTGTGAAACAGGAGCTGATAGGAGATATAGTTACCAAGGGCAATATCCGTCTGGCCCTCCAGGATGTCCAGGACACTGTACACCAGAACTATAGCTTCCCAGACAAAAGTTTCAAGTATGAGCAGAAATTCCAAAATGGGAGATGGGGAGGAAACCTGTTGGGATATTTTGTTCTTTGTCAAAAGTTGATGAAGTCAAAATGCCAAACGGAAGCTCTCCTTGCTCCAGCCATGCATGTCCACCCCAAGCTTCCGGAATATGATGATGTGGTGGCCAAACTTTGCCGTCTCAAGGCAGAACATAGTAGGCGTAAGACCTTTGCATTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

252

Amino Acids

29.03

Weight (kDa)

9.67

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 89 - 153 2.4e-15 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15490
fragaria_vesca FvH4_3g20621
malus_domestica MD03G1223500.v1.1
prunus_persica Prupe.4G186000_v2.0.a1 Prupe.4G186000_v2.0.a1
rosa_laevigata RLG00000033564
rosa_multiflora Rmu_sc0002460.1_g000083
rosa_roxburghii Rroxscaffold_1G00045670
rosa_samantha Rh5BG240200 Rh5CG270600 Rh5DG248400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 676
AciI CCGC 1 cut(s) 144
AclWI GGATC 1 cut(s) 388
AcoI YGGCCR 2 cut(s) 111, 696
AcsI RAATTY 3 cut(s) 9, 386, 551
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 2 cut(s) 131, 715
AgsI TTSAA 3 cut(s) 96, 367, 538
AjnI CCWGG 2 cut(s) 483, 492
AloI GAACNNNNNNTCC 2 cut(s) 579, 611
AluBI AGCT 5 cut(s) 343, 439, 519, 638, 673
AluI AGCT 5 cut(s) 343, 439, 519, 638, 673
Alw26I GTCTC 1 cut(s) 716
AlwI GGATC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 439
Aor13HI TCCGGA 1 cut(s) 676
AoxI GGCC 4 cut(s) 111, 173, 477, 696
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 3 cut(s) 9, 386, 551
AsiSI GCGATCGC 1 cut(s) 297
AspS9I GGNCC 4 cut(s) 4, 174, 258, 478
AvaII GGWCC 2 cut(s) 4, 258
BalI TGGCCA 2 cut(s) 113, 698
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 301
BccI CCATC 2 cut(s) 195, 561
BceAI ACGGC 3 cut(s) 188, 428, 693
BcgI CGANNNNNNTGC 2 cut(s) 339, 373
BciT130I CCWGG 2 cut(s) 485, 494
BcoDI GTCTC 1 cut(s) 716
BfmI CTRYAG 1 cut(s) 514
BisI GCNGC 1 cut(s) 315
BlsI GCNGC 1 cut(s) 316
Bme1390I CCNGG 2 cut(s) 485, 494
Bme18I GGWCC 2 cut(s) 4, 258
BmgT120I GGNCC 4 cut(s) 4, 174, 258, 478
BmrFI CCNGG 2 cut(s) 485, 494
BpmI CTGGAG 2 cut(s) 467, 633
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 2 cut(s) 304, 698
BsaJI CCNNGG 1 cut(s) 459
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 715
Bse3DI GCAATG 1 cut(s) 228
BseAI TCCGGA 1 cut(s) 676
BseBI CCWGG 2 cut(s) 485, 494
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 493
BseLI CCNNNNNNNGG 2 cut(s) 131, 715
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 156
BseXI GCAGC 1 cut(s) 301
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 4 cut(s) 113, 175, 479, 698
BsiEI CGRYCG 1 cut(s) 297
BsiSI CCGG 1 cut(s) 677
BslI CCNNNNNNNGG 2 cut(s) 131, 715
BsmAI GTCTC 1 cut(s) 716
BsmBI CGTCTC 1 cut(s) 716
BsnI GGCC 4 cut(s) 113, 175, 479, 698
Bsp13I TCCGGA 1 cut(s) 676
Bsp1407I TGTACA 1 cut(s) 502
Bsp143I GATC 2 cut(s) 294, 380
BspACI CCGC 1 cut(s) 144
BspANI GGCC 4 cut(s) 113, 175, 479, 698
BspCNI CTCAG 1 cut(s) 157
BspEI TCCGGA 1 cut(s) 676
BspPI GGATC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 228
BsrGI TGTACA 1 cut(s) 502
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 2 cut(s) 294, 380
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 2 cut(s) 485, 494
Bst4CI ACNGT 2 cut(s) 51, 502
BstAPI GCANNNNNTGC 1 cut(s) 271
BstAUI TGTACA 1 cut(s) 502
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 2 cut(s) 57, 165
BstF5I GGATG 1 cut(s) 493
BstKTI GATC 2 cut(s) 297, 383
BstMAI GTCTC 1 cut(s) 716
BstMBI GATC 2 cut(s) 294, 380
BstMCI CGRYCG 1 cut(s) 297
BstMWI GCNNNNNNNGC 2 cut(s) 271, 644
BstNI CCWGG 2 cut(s) 485, 494
BstNSI RCATGY 1 cut(s) 662
BstSCI CCNGG 2 cut(s) 483, 492
BstSFI CTRYAG 1 cut(s) 514
BstV1I GCAGC 1 cut(s) 301
BsuRI GGCC 4 cut(s) 113, 175, 479, 698
BtsCI GGATG 1 cut(s) 493
BtsIMutI CAGTG 2 cut(s) 47, 498
Cac8I GCNNGC 1 cut(s) 122
CaiI CAGNNNCTG 1 cut(s) 439
Cfr13I GGNCC 4 cut(s) 4, 174, 258, 478
Csp6I GTAC 1 cut(s) 503
CspCI CAANNNNNGTGG 2 cut(s) 211, 246
CviAII CATG 3 cut(s) 416, 655, 659
CviQI GTAC 1 cut(s) 503
DdeI CTNAG 2 cut(s) 57, 165
DpnI GATC 2 cut(s) 296, 382
DpnII GATC 2 cut(s) 294, 380
EaeI YGGCCR 2 cut(s) 111, 696
Eco130I CCWWGG 1 cut(s) 459
Eco47I GGWCC 2 cut(s) 4, 258
EcoO109I RGGNCCY 1 cut(s) 258
EcoRII CCWGG 2 cut(s) 483, 492
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 716
FaeI CATG 3 cut(s) 419, 658, 662
FalI AAGNNNNNCTT 2 cut(s) 627, 659
FatI CATG 3 cut(s) 415, 654, 658
FauI CCCGC 1 cut(s) 137
Fnu4HI GCNGC 1 cut(s) 315
FokI GGATG 1 cut(s) 500
Fsp4HI GCNGC 1 cut(s) 315
GluI GCNGC 1 cut(s) 315
GsuI CTGGAG 2 cut(s) 467, 633
HaeIII GGCC 4 cut(s) 113, 175, 479, 698
HapII CCGG 1 cut(s) 677
Hin1II CATG 3 cut(s) 419, 658, 662
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 2 cut(s) 192, 421
HpaII CCGG 1 cut(s) 677
Hpy166II GTNNAC 2 cut(s) 505, 664
Hpy188I TCNGA 2 cut(s) 385, 755
Hpy188III TCNNGA 2 cut(s) 321, 677
Hpy8I GTNNAC 2 cut(s) 505, 664
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 2 cut(s) 51, 502
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 6 cut(s) 120, 202, 208, 265, 658, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 271, 644
HpyF3I CTNAG 2 cut(s) 57, 165
HpySE526I ACGT 1 cut(s) 355
Hsp92II CATG 3 cut(s) 419, 658, 662
Kpn2I TCCGGA 1 cut(s) 676
Kzo9I GATC 2 cut(s) 294, 380
LmnI GCTCC 2 cut(s) 436, 652
Lsp1109I GCAGC 1 cut(s) 301
MaeII ACGT 1 cut(s) 355
MaeIII GTNAC 1 cut(s) 454
MalI GATC 2 cut(s) 296, 382
MboI GATC 2 cut(s) 294, 380
MfeI CAATTG 1 cut(s) 203
MlsI TGGCCA 2 cut(s) 113, 698
MluCI AATT 6 cut(s) 9, 23, 203, 332, 386, 551
MluNI TGGCCA 2 cut(s) 113, 698
MlyI GAGTC 1 cut(s) 186
MnlI CCTC 5 cut(s) 149, 160, 328, 491, 566
Mox20I TGGCCA 2 cut(s) 113, 698
Mph1103I ATGCAT 1 cut(s) 660
MroI TCCGGA 1 cut(s) 676
MscI TGGCCA 2 cut(s) 113, 698
MseI TTAA 2 cut(s) 29, 405
MslI CAYNNNNRTG 1 cut(s) 79
Msp20I TGGCCA 2 cut(s) 113, 698
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 2 cut(s) 485, 494
MunI CAATTG 1 cut(s) 203
MvaI CCWGG 2 cut(s) 485, 494
MwoI GCNNNNNNNGC 2 cut(s) 271, 644
NdeII GATC 2 cut(s) 294, 380
NlaIII CATG 3 cut(s) 419, 658, 662
NmeAIII GCCGAG 1 cut(s) 300
NsiI ATGCAT 1 cut(s) 660
NspI RCATGY 1 cut(s) 662
PfeI GAWTC 1 cut(s) 421
PfoI TCCNGGA 2 cut(s) 483, 492
PkrI GCNGC 1 cut(s) 316
Ple19I CGATCG 1 cut(s) 297
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
PpuMI RGGWCCY 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 483, 492
PspGI CCWGG 2 cut(s) 483, 492
PspPI GGNCC 4 cut(s) 4, 174, 258, 478
PspPPI RGGWCCY 1 cut(s) 258
PstNI CAGNNNCTG 1 cut(s) 439
PvuI CGATCG 1 cut(s) 297
RgaI GCGATCGC 1 cut(s) 297
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 79
SaqAI TTAA 2 cut(s) 29, 405
SatI GCNGC 1 cut(s) 315
Sau3AI GATC 2 cut(s) 294, 380
Sau96I GGNCC 4 cut(s) 4, 174, 258, 478
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 2 cut(s) 485, 494
SetI ASST 9 cut(s) 263, 345, 358, 441, 521, 582, 640, 675, 743
SfaAI GCGATCGC 1 cut(s) 297
SfcI CTRYAG 1 cut(s) 514
SgfI GCGATCGC 1 cut(s) 297
SinI GGWCC 2 cut(s) 4, 258
SmiMI CAYNNNNRTG 1 cut(s) 79
SmlI CTYRAG 2 cut(s) 319, 713
SmoI CTYRAG 2 cut(s) 319, 713
Sse9I AATT 6 cut(s) 9, 23, 203, 332, 386, 551
SsiI CCGC 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 483, 492
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 51, 502
TaiI ACGT 1 cut(s) 358
TaqI TCGA 2 cut(s) 136, 190
TasI AATT 6 cut(s) 9, 23, 203, 332, 386, 551
TatI WGTACW 1 cut(s) 502
TfiI GAWTC 1 cut(s) 421
Tru1I TTAA 2 cut(s) 29, 405
Tru9I TTAA 2 cut(s) 29, 405
TscAI CASTG 2 cut(s) 54, 505
TseI GCWGC 1 cut(s) 314
TspDTI ATGAA 2 cut(s) 169, 629
TspGWI ACGGA 2 cut(s) 461, 647
TspRI CASTG 2 cut(s) 54, 505
VpaK11BI GGWCC 2 cut(s) 4, 258
XapI RAATTY 3 cut(s) 9, 386, 551
XceI RCATGY 1 cut(s) 662
Zsp2I ATGCAT 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.