Prupe.4G186000_v2.0.a1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017928
Physical Location & Seq
Forward (+)
11042580 .. 11045174
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G186000.2

Sequence Viewer

Length: 1182 bp
ATGTGGTCTGGTGCACTTCGAGCTGCGCTTATATGGACCAAGGCTTCAAGATGGAGGAAAGTATCCAAATGCAAGAGCCTGGTTAAGCAAGTTCAAATGCACTTAACCATCCAAAAGAACAGGAGAGACGCTATTATAAGGCAGGCACGTGTGGACATTGCCCAGCTTCTTCAAAATGGCCAGCCACAGCAAGCTTTAGCTAGGGTTGAGACACTCCAGAAGGACCAGTGCCTTTTGGCTGCTTATGATCAAATTGACCATTTCTGCAGTTGCATAAGCATCAGCATTGTCCATGTATTCAAAAACAAGACTGTGCAAGATTTGCCTAGTAGTGTTGGAGAAGCAATGGCAAGTTTGATATTTGCTGCCTCAAGATGTGGTGAACTGCCAGAGCTGCGTTTGCTGCGTGGCCTGTTTACAGAGCAATATGGATGGGAATTTGACATTACCAGCGTTGAATTGCGACCTGGGAATCTTGTAAATTCTCAGTTGAAGGAGAAACTATGCATAAATCTGATTCCCGATGATGTGAAACAAGGGTTATTAAGTGATCTAGTAAACAAGAGCAGTGTTCAGATGACGCTCCAGGATCGACCAGGCACCGCACGTCAGAAATATAGCTTTTCAGACAAAGATTTCAAGGATAAGCAGAAATTTGATGGGATTCAAATACAAATTCCAAAATTGGAGACAGCGAGGAAAACTGGGATAGTCTGTTCTCTGTCAAACGGTAAGTTCTCAGTGGCTACTCCATCCCATTCTCAAATTTGCTCAAATACTACTTATGGTTTAGGTGACTCAACCAAAACCAAGAAAAATGAAGATCAAGATCATCGCAGCAATGGAAACATACATATAAATATTGACTCAGGGGGTAGAAATGCAAGCTTTAGAACATATGCCATGACTAGTTTACCTAGAAAAAGCCCAACTGGGAAAATGGTTCCGTCCAAAATCCGGTATGACCATCTCTCCCCGATGGATGCTCCGAACAAAGATCGTTCCAAGATGTTGGCGAAGGGTCGCCATTCTTTCAGTAGTTCGGCTGACGAACATAAAATCGCAAAGGGAATATCTCCTTGCCATGTCCACCCCAAGCTTCCAGAATATGACGATCTAGTGGCCAAACTTCGTTCTCTCAAGGCAGAGTATAGGCATCATAAAAGCTTCTCGTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

394

Amino Acids

44.2

Weight (kDa)

9.65

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15490
fragaria_vesca FvH4_3g20621
malus_domestica MD03G1223500.v1.1
prunus_persica Prupe.4G186000_v2.0.a1 Prupe.4G186000_v2.0.a1
rosa_laevigata RLG00000033564
rosa_multiflora Rmu_sc0002460.1_g000083
rosa_roxburghii Rroxscaffold_1G00045670
rosa_samantha Rh5BG240200 Rh5CG270600 Rh5DG248400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 599
AciI CCGC 1 cut(s) 603
AclWI GGATC 1 cut(s) 597
AcoI YGGCCR 2 cut(s) 178, 1122
AcsI RAATTY 5 cut(s) 437, 481, 653, 675, 765
AcvI CACGTG 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 957
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 8 cut(s) 48, 95, 173, 301, 458, 493, 640, 668
AhlI ACTAGT 1 cut(s) 908
AjiI CACGTC 1 cut(s) 608
AjnI CCWGG 4 cut(s) 78, 466, 585, 595
AloI GAACNNNNNNTCC 2 cut(s) 700, 732
AluBI AGCT 9 cut(s) 23, 166, 194, 200, 394, 621, 888, 1099, 1167
AluI AGCT 9 cut(s) 23, 166, 194, 200, 394, 621, 888, 1099, 1167
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 3 cut(s) 120, 203, 683
Alw44I GTGCAC 1 cut(s) 12
AlwI GGATC 1 cut(s) 597
AoxI GGCC 3 cut(s) 178, 409, 1122
ApaLI GTGCAC 1 cut(s) 12
ApeKI GCWGC 6 cut(s) 23, 239, 365, 394, 403, 837
ApoI RAATTY 5 cut(s) 437, 481, 653, 675, 765
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 2 cut(s) 36, 223
AsuHPI GGTGA 2 cut(s) 392, 806
AvaII GGWCC 2 cut(s) 36, 223
BaeGI GKGCMC 1 cut(s) 16
BalI TGGCCA 2 cut(s) 180, 1124
BanI GGYRCC 1 cut(s) 599
BbrPI CACGTG 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 6 cut(s) 10, 226, 352, 381, 390, 849
BccI CCATC 7 cut(s) 45, 116, 426, 653, 760, 973, 975
BciT130I CCWGG 4 cut(s) 80, 468, 587, 597
BciVI GTATCC 1 cut(s) 73
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 3 cut(s) 120, 203, 683
BcuI ACTAGT 1 cut(s) 908
BfaI CTAG 6 cut(s) 201, 327, 554, 909, 918, 1118
BfmI CTRYAG 1 cut(s) 265
BfuI GTATCC 1 cut(s) 73
BisI GCNGC 6 cut(s) 24, 240, 366, 395, 404, 838
BlsI GCNGC 6 cut(s) 25, 241, 367, 396, 405, 839
Bme1390I CCNGG 4 cut(s) 80, 468, 587, 597
Bme18I GGWCC 2 cut(s) 36, 223
BmgBI CACGTC 1 cut(s) 608
BmgT120I GGNCC 2 cut(s) 36, 223
BmiI GGNNCC 2 cut(s) 601, 945
BmrFI CCNGG 4 cut(s) 80, 468, 587, 597
BmrI ACTGGG 2 cut(s) 714, 942
BmsI GCATC 3 cut(s) 288, 973, 1165
BmuI ACTGGG 2 cut(s) 714, 942
BpmI CTGGAG 2 cut(s) 200, 569
BpuEI CTTGAG 2 cut(s) 355, 1124
BsaAI YACGTR 1 cut(s) 149
BsaBI GATNNNNATC 1 cut(s) 828
BsaJI CCNNGG 2 cut(s) 39, 467
BsaWI WCCGGW 1 cut(s) 957
BsaXI ACNNNNNCTCC 2 cut(s) 956, 986
Bsc4I CCNNNNNNNGG 1 cut(s) 957
Bse1I ACTGG 3 cut(s) 226, 709, 937
Bse3DI GCAATG 3 cut(s) 156, 351, 847
Bse8I GATNNNNATC 1 cut(s) 828
BseBI CCWGG 4 cut(s) 80, 468, 587, 597
BseDI CCNNGG 2 cut(s) 39, 467
BseGI GGATG 4 cut(s) 108, 437, 752, 988
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 1 cut(s) 957
BseMI GCAATG 3 cut(s) 156, 351, 847
BseMII CTCAG 3 cut(s) 500, 753, 882
BseNI ACTGG 3 cut(s) 226, 709, 937
BseSI GKGCMC 1 cut(s) 16
BseXI GCAGC 6 cut(s) 10, 226, 352, 381, 390, 849
BseYI CCCAGC 1 cut(s) 162
BshFI GGCC 3 cut(s) 180, 411, 1124
BshNI GGYRCC 1 cut(s) 599
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 1 cut(s) 958
BslI CCNNNNNNNGG 1 cut(s) 957
BsmAI GTCTC 3 cut(s) 120, 203, 683
BsmBI CGTCTC 1 cut(s) 120
BsnI GGCC 3 cut(s) 180, 411, 1124
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 7 cut(s) 247, 550, 589, 823, 829, 997, 1114
BspACI CCGC 1 cut(s) 603
BspANI GGCC 3 cut(s) 180, 411, 1124
BspCNI CTCAG 3 cut(s) 499, 752, 881
BspLI GGNNCC 2 cut(s) 601, 945
BspMAI CTGCAG 1 cut(s) 269
BspPI GGATC 1 cut(s) 597
BspT107I GGYRCC 1 cut(s) 599
BsrDI GCAATG 3 cut(s) 156, 351, 847
BsrI ACTGG 3 cut(s) 226, 709, 937
BssECI CCNNGG 2 cut(s) 39, 467
BssMI GATC 7 cut(s) 247, 550, 589, 823, 829, 997, 1114
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 4 cut(s) 80, 468, 587, 597
Bst4CI ACNGT 2 cut(s) 313, 731
BstAPI GCANNNNNTGC 1 cut(s) 322
BstBAI YACGTR 1 cut(s) 149
BstC8I GCNNGC 4 cut(s) 144, 182, 192, 886
BstDEI CTNAG 3 cut(s) 486, 739, 868
BstF5I GGATG 4 cut(s) 108, 437, 752, 988
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 7 cut(s) 250, 553, 592, 826, 832, 1000, 1117
BstMAI GTCTC 3 cut(s) 120, 203, 683
BstMBI GATC 7 cut(s) 247, 550, 589, 823, 829, 997, 1114
BstMWI GCNNNNNNNGC 5 cut(s) 20, 322, 394, 400, 403
BstNI CCWGG 4 cut(s) 80, 468, 587, 597
BstSCI CCNGG 4 cut(s) 78, 466, 585, 595
BstSFI CTRYAG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 16
BstV1I GCAGC 6 cut(s) 10, 226, 352, 381, 390, 849
BstXI CCANNNNNNTGG 1 cut(s) 1012
BsuI GTATCC 1 cut(s) 73
BsuRI GGCC 3 cut(s) 180, 411, 1124
BtgZI GCGATG 1 cut(s) 818
BtrI CACGTC 1 cut(s) 608
BtsCI GGATG 4 cut(s) 108, 437, 752, 988
BtsI GCAGTG 1 cut(s) 574
BtsIMutI CAGTG 3 cut(s) 233, 574, 747
Cac8I GCNNGC 4 cut(s) 144, 182, 192, 886
CfoI GCGC 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 36, 223
CseI GACGC 2 cut(s) 137, 589
CviAII CATG 3 cut(s) 293, 904, 1085
DdeI CTNAG 3 cut(s) 486, 739, 868
DpnI GATC 7 cut(s) 249, 552, 591, 825, 831, 999, 1116
DpnII GATC 7 cut(s) 247, 550, 589, 823, 829, 997, 1114
EaeI YGGCCR 2 cut(s) 178, 1122
Eco130I CCWWGG 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 36, 223
Eco72I CACGTG 1 cut(s) 149
EcoRII CCWGG 4 cut(s) 78, 466, 585, 595
EcoT14I CCWWGG 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 509
ErhI CCWWGG 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 120
FaeI CATG 3 cut(s) 296, 907, 1088
FatI CATG 3 cut(s) 292, 903, 1084
FauNDI CATATG 1 cut(s) 898
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 6 cut(s) 24, 240, 366, 395, 404, 838
FokI GGATG 4 cut(s) 95, 444, 739, 995
Fsp4HI GCNGC 6 cut(s) 24, 240, 366, 395, 404, 838
FspBI CTAG 6 cut(s) 201, 327, 554, 909, 918, 1118
GlaI GCGC 1 cut(s) 27
GluI GCNGC 6 cut(s) 24, 240, 366, 395, 404, 838
GsaI CCCAGC 1 cut(s) 166
GsuI CTGGAG 2 cut(s) 200, 569
HaeIII GGCC 3 cut(s) 180, 411, 1124
HapII CCGG 1 cut(s) 958
HgaI GACGC 2 cut(s) 137, 589
HhaI GCGC 1 cut(s) 28
Hin1II CATG 3 cut(s) 296, 907, 1088
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HindIII AAGCTT 4 cut(s) 192, 886, 1097, 1165
HinfI GANTC 5 cut(s) 472, 517, 664, 797, 866
HpaII CCGG 1 cut(s) 958
HphI GGTGA 2 cut(s) 392, 806
Hpy166II GTNNAC 7 cut(s) 14, 154, 383, 417, 559, 914, 1090
Hpy188I TCNGA 6 cut(s) 516, 576, 612, 628, 990, 1181
Hpy188III TCNNGA 6 cut(s) 48, 217, 372, 521, 827, 1103
Hpy8I GTNNAC 7 cut(s) 14, 154, 383, 417, 559, 914, 1090
HpyAV CCTTC 3 cut(s) 214, 487, 1012
HpyCH4III ACNGT 2 cut(s) 313, 731
HpyCH4IV ACGT 2 cut(s) 148, 607
HpyCH4V TGCA 8 cut(s) 14, 72, 100, 267, 273, 316, 507, 884
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 322, 394, 400, 403
HpyF3I CTNAG 3 cut(s) 486, 739, 868
HpySE526I ACGT 2 cut(s) 148, 607
Hsp92II CATG 3 cut(s) 296, 907, 1088
HspAI GCGC 1 cut(s) 26
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 7 cut(s) 247, 550, 589, 823, 829, 997, 1114
LmnI GCTCC 2 cut(s) 588, 991
Lsp1109I GCAGC 6 cut(s) 10, 226, 352, 381, 390, 849
LweI GCATC 3 cut(s) 288, 973, 1165
MaeI CTAG 6 cut(s) 201, 327, 554, 909, 918, 1118
MaeII ACGT 2 cut(s) 148, 607
MaeIII GTNAC 1 cut(s) 794
MalI GATC 7 cut(s) 249, 552, 591, 825, 831, 999, 1116
MboI GATC 7 cut(s) 247, 550, 589, 823, 829, 997, 1114
MboII GAAGA 2 cut(s) 161, 833
MhlI GDGCHC 1 cut(s) 16
MlsI TGGCCA 2 cut(s) 180, 1124
MluCI AATT 8 cut(s) 252, 437, 458, 481, 653, 675, 683, 765
MluNI TGGCCA 2 cut(s) 180, 1124
MlyI GAGTC 2 cut(s) 791, 860
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 3 cut(s) 48, 379, 690
Mox20I TGGCCA 2 cut(s) 180, 1124
Mph1103I ATGCAT 1 cut(s) 509
MscI TGGCCA 2 cut(s) 180, 1124
MseI TTAA 3 cut(s) 84, 104, 545
Msp20I TGGCCA 2 cut(s) 180, 1124
MspI CCGG 1 cut(s) 958
MspR9I CCNGG 4 cut(s) 80, 468, 587, 597
MvaI CCWGG 4 cut(s) 80, 468, 587, 597
MwoI GCNNNNNNNGC 5 cut(s) 20, 322, 394, 400, 403
NdeI CATATG 1 cut(s) 898
NdeII GATC 7 cut(s) 247, 550, 589, 823, 829, 997, 1114
NlaIII CATG 3 cut(s) 296, 907, 1088
NlaIV GGNNCC 2 cut(s) 601, 945
NmuCI GTSAC 1 cut(s) 794
NsiI ATGCAT 1 cut(s) 509
PfeI GAWTC 3 cut(s) 472, 517, 664
PfoI TCCNGGA 1 cut(s) 585
PkrI GCNGC 6 cut(s) 25, 241, 367, 396, 405, 839
PleI GAGTC 2 cut(s) 791, 860
PmaCI CACGTG 1 cut(s) 149
PmlI CACGTG 1 cut(s) 149
PpsI GAGTC 2 cut(s) 791, 860
Ppu21I YACGTR 1 cut(s) 149
PsiI TTATAA 1 cut(s) 137
Psp6I CCWGG 4 cut(s) 78, 466, 585, 595
PspCI CACGTG 1 cut(s) 149
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 4 cut(s) 78, 466, 585, 595
PspN4I GGNNCC 2 cut(s) 601, 945
PspPI GGNCC 2 cut(s) 36, 223
PstI CTGCAG 1 cut(s) 269
SaqAI TTAA 3 cut(s) 84, 104, 545
SatI GCNGC 6 cut(s) 24, 240, 366, 395, 404, 838
Sau3AI GATC 7 cut(s) 247, 550, 589, 823, 829, 997, 1114
Sau96I GGNCC 2 cut(s) 36, 223
SchI GAGTC 2 cut(s) 791, 860
ScrFI CCNGG 4 cut(s) 80, 468, 587, 597
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 3 cut(s) 288, 973, 1165
SfcI CTRYAG 1 cut(s) 265
SinI GGWCC 2 cut(s) 36, 223
SmlI CTYRAG 2 cut(s) 370, 1139
SmoI CTYRAG 2 cut(s) 370, 1139
SpeI ACTAGT 1 cut(s) 908
Sse9I AATT 8 cut(s) 252, 437, 458, 481, 653, 675, 683, 765
SsiI CCGC 1 cut(s) 603
SspI AATATT 1 cut(s) 862
SspMI CTAG 6 cut(s) 201, 327, 554, 909, 918, 1118
StyD4I CCNGG 4 cut(s) 78, 466, 585, 595
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 2 cut(s) 313, 731
TaiI ACGT 2 cut(s) 151, 610
TaqI TCGA 2 cut(s) 19, 592
TasI AATT 8 cut(s) 252, 437, 458, 481, 653, 675, 683, 765
TfiI GAWTC 3 cut(s) 472, 517, 664
Tru1I TTAA 3 cut(s) 84, 104, 545
Tru9I TTAA 3 cut(s) 84, 104, 545
TscAI CASTG 3 cut(s) 233, 574, 747
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 6 cut(s) 23, 239, 365, 394, 403, 837
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 1 cut(s) 834
TspGWI ACGGA 1 cut(s) 936
TspRI CASTG 3 cut(s) 233, 574, 747
VneI GTGCAC 1 cut(s) 12
VpaK11BI GGWCC 2 cut(s) 36, 223
XapI RAATTY 5 cut(s) 437, 481, 653, 675, 765
XspI CTAG 6 cut(s) 201, 327, 554, 909, 918, 1118
Zsp2I ATGCAT 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.