MD03G1223500.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0017928
Physical Location & Seq
Reverse (-)
30846716 .. 30849334
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1223500.v1.1.491

Sequence Viewer

Length: 990 bp
ATGTGGTATGGTGCACTCCGAGCTGCAATTATATGGACCAAGGCTTCAAGATGGAGAAAAGTGTCCAAATGCAAGAGCCTGGTTACGCAAGTGCAGAAGCATTTAACCATCCAAAAGAATAGGAGAGATGCTATTGTGAGGCAGGCACGTGTTGACATCTCTCTGCTTCTCCGAAATGGACAGCTAGAGCAAGCTTTACCTAGGGACTTGCCTGGTGCTGTTGGAGAAGCAGTTGCAAGTTTAATATTTGCTGCCTCAAGATGTGGTGAACTGCCAGAGCTACGTCTGTTGCGTGGCCTGTTCAAAGAGCAATACGGATGGGAACTCGACGTTATCAGTGTCGAATTACGTGCTGGGAATCTTGTGAATTCTCAGTTGAAGGAGAAACTATGCATAACTCTGATTCCGGACGAAGTGAAACAGTATTTAATAGGTGATATAATGAAGAACACCAATGTCCAGCTGGCGCTTCAGGATAGCCAAGGCACGACACACCAGAATTATAGCTTTGCAGACAGAGATTTCAAGGATGAGCAGAAAGCTGATGGGATTCAAATACAAATTCCGAATTCGGAAACGGAGGAGAGTTCTCGGATACGTTGTTCGCTGGCGAACGGTAAGCTTTCACTGGCCACGCTATGCCATTTTCATAATGGCTCAAGTACAACTGCTGGTTCAGTTTGCTTAAACAAAATCAAGAAAAATGATATCGACTTACGGGACGGAAATGGAAACCCTAGGACACATGCCATGACTACTACACCTAGAAAAAGTACAAATGGGGATGCTGTGAACAAAGATCGCTCCAAGATGTTGGTGAGGAGTCGCAATTCTTTCGCTGGTCCGGTTGATGGACATAAAATGGCGCAGGGAACGTCTCCTTGCTCCAGCCATGTCCACCCCAAGCTTCCGGAATATGATGATCTAGTAGCCAAACTGAAGTGTCTCAAGGCAGAGTATAGGCATCGTAAAAACTTTTCAGTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

330

Amino Acids

36.81

Weight (kDa)

9.57

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 69 - 154 2.6e-15 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15490
fragaria_vesca FvH4_3g20621
malus_domestica MD03G1223500.v1.1
prunus_persica Prupe.4G186000_v2.0.a1 Prupe.4G186000_v2.0.a1
rosa_laevigata RLG00000033564
rosa_multiflora Rmu_sc0002460.1_g000083
rosa_roxburghii Rroxscaffold_1G00045670
rosa_samantha Rh5BG240200 Rh5CG270600 Rh5DG248400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 406, 910
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 3 cut(s) 367, 561, 568
AcuI CTGAAG 2 cut(s) 455, 959
AcvI CACGTG 1 cut(s) 149
AfaI GTAC 2 cut(s) 664, 775
AfiI CCNNNNNNNGG 1 cut(s) 851
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 5 cut(s) 48, 304, 379, 526, 554
AjnI CCWGG 2 cut(s) 78, 211
AloI GAACNNNNNNTCC 2 cut(s) 586, 618
AluBI AGCT 9 cut(s) 23, 184, 194, 280, 463, 507, 542, 622, 907
AluI AGCT 9 cut(s) 23, 184, 194, 280, 463, 507, 542, 622, 907
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 2 cut(s) 882, 950
Alw44I GTGCAC 1 cut(s) 12
Aor13HI TCCGGA 2 cut(s) 406, 910
AoxI GGCC 2 cut(s) 295, 630
ApaLI GTGCAC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 23, 251
ApoI RAATTY 3 cut(s) 367, 561, 568
AspA2I CCTAGG 2 cut(s) 200, 737
AspLEI GCGC 2 cut(s) 469, 868
AspS9I GGNCC 2 cut(s) 36, 842
AsuHPI GGTGA 3 cut(s) 278, 446, 829
AvaII GGWCC 2 cut(s) 36, 842
AvrII CCTAGG 2 cut(s) 200, 737
BaeGI GKGCMC 1 cut(s) 16
BalI TGGCCA 1 cut(s) 632
BbrPI CACGTG 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 10, 238
BccI CCATC 5 cut(s) 45, 116, 312, 539, 845
BcgI CGANNNNNNTGC 4 cut(s) 332, 366, 817, 851
BciT130I CCWGG 2 cut(s) 80, 213
BciVI GTATCC 1 cut(s) 588
BcoDI GTCTC 2 cut(s) 882, 950
BfaI CTAG 5 cut(s) 185, 201, 738, 765, 926
BfoI RGCGCY 1 cut(s) 470
BfuI GTATCC 1 cut(s) 588
BisI GCNGC 2 cut(s) 24, 252
BlnI CCTAGG 2 cut(s) 200, 737
BlsI GCNGC 2 cut(s) 25, 253
Bme1390I CCNGG 2 cut(s) 80, 213
Bme18I GGWCC 2 cut(s) 36, 842
BmgT120I GGNCC 2 cut(s) 36, 842
BmrFI CCNGG 2 cut(s) 80, 213
BmsI GCATC 3 cut(s) 118, 775, 973
BpmI CTGGAG 1 cut(s) 871
BpuEI CTTGAG 3 cut(s) 241, 643, 932
BsaAI YACGTR 2 cut(s) 149, 350
BsaJI CCNNGG 4 cut(s) 39, 200, 481, 737
BsaWI WCCGGW 3 cut(s) 406, 844, 910
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 851
Bse1I ACTGG 1 cut(s) 633
BseAI TCCGGA 2 cut(s) 406, 910
BseBI CCWGG 2 cut(s) 80, 213
BseDI CCNNGG 4 cut(s) 39, 200, 481, 737
BseGI GGATG 4 cut(s) 108, 323, 535, 790
BseLI CCNNNNNNNGG 1 cut(s) 851
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 1 cut(s) 633
BseRI GAGGAG 2 cut(s) 596, 835
BseSI GKGCMC 1 cut(s) 16
BseXI GCAGC 2 cut(s) 10, 238
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 113
BshFI GGCC 2 cut(s) 297, 632
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 3 cut(s) 407, 845, 911
BslFI GGGAC 2 cut(s) 218, 734
BslI CCNNNNNNNGG 1 cut(s) 851
BsmAI GTCTC 2 cut(s) 882, 950
BsmBI CGTCTC 1 cut(s) 882
BsmFI GGGAC 2 cut(s) 218, 734
BsnI GGCC 2 cut(s) 297, 632
Bsp1286I GDGCHC 1 cut(s) 16
Bsp13I TCCGGA 2 cut(s) 406, 910
Bsp143I GATC 2 cut(s) 799, 922
BspANI GGCC 2 cut(s) 297, 632
BspCNI CTCAG 1 cut(s) 385
BspEI TCCGGA 2 cut(s) 406, 910
BsrI ACTGG 1 cut(s) 633
BssECI CCNNGG 4 cut(s) 39, 200, 481, 737
BssMI GATC 2 cut(s) 799, 922
BssT1I CCWWGG 4 cut(s) 39, 200, 481, 737
Bst2UI CCWGG 2 cut(s) 80, 213
Bst4CI ACNGT 2 cut(s) 423, 617
BstBAI YACGTR 2 cut(s) 149, 350
BstC8I GCNNGC 4 cut(s) 144, 192, 465, 609
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 4 cut(s) 108, 323, 535, 790
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 2 cut(s) 469, 868
BstKTI GATC 2 cut(s) 802, 925
BstMAI GTCTC 2 cut(s) 882, 950
BstMBI GATC 2 cut(s) 799, 922
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 2 cut(s) 80, 213
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 2 cut(s) 78, 211
BstSLI GKGCMC 1 cut(s) 16
BstV1I GCAGC 2 cut(s) 10, 238
BstXI CCANNNNNNTGG 1 cut(s) 814
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 2 cut(s) 297, 632
BtsCI GGATG 4 cut(s) 108, 323, 535, 790
BtsIMutI CAGTG 2 cut(s) 343, 626
Cac8I GCNNGC 4 cut(s) 144, 192, 465, 609
CfoI GCGC 2 cut(s) 469, 868
Cfr13I GGNCC 2 cut(s) 36, 842
Csp6I GTAC 2 cut(s) 663, 774
CviAII CATG 3 cut(s) 746, 751, 893
CviQI GTAC 2 cut(s) 663, 774
DdeI CTNAG 1 cut(s) 372
DpnI GATC 2 cut(s) 801, 924
DpnII GATC 2 cut(s) 799, 922
EaeI YGGCCR 1 cut(s) 630
Eco130I CCWWGG 4 cut(s) 39, 200, 481, 737
Eco32I GATATC 1 cut(s) 709
Eco47I GGWCC 2 cut(s) 36, 842
Eco57I CTGAAG 2 cut(s) 455, 959
Eco72I CACGTG 1 cut(s) 149
EcoRI GAATTC 2 cut(s) 367, 568
EcoRII CCWGG 2 cut(s) 78, 211
EcoRV GATATC 1 cut(s) 709
EcoT14I CCWWGG 4 cut(s) 39, 200, 481, 737
EcoT22I ATGCAT 1 cut(s) 395
ErhI CCWWGG 4 cut(s) 39, 200, 481, 737
Esp3I CGTCTC 1 cut(s) 882
FaeI CATG 3 cut(s) 749, 754, 896
FaqI GGGAC 2 cut(s) 218, 734
FatI CATG 3 cut(s) 745, 750, 892
Fnu4HI GCNGC 2 cut(s) 24, 252
FokI GGATG 4 cut(s) 95, 330, 542, 797
Fsp4HI GCNGC 2 cut(s) 24, 252
FspBI CTAG 5 cut(s) 185, 201, 738, 765, 926
GlaI GCGC 2 cut(s) 468, 867
GluI GCNGC 2 cut(s) 24, 252
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 871
HaeII RGCGCY 1 cut(s) 470
HaeIII GGCC 2 cut(s) 297, 632
HapII CCGG 3 cut(s) 407, 845, 911
HhaI GCGC 2 cut(s) 469, 868
Hin1II CATG 3 cut(s) 749, 754, 896
Hin6I GCGC 2 cut(s) 467, 866
HinP1I GCGC 2 cut(s) 467, 866
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 3 cut(s) 192, 620, 905
HinfI GANTC 4 cut(s) 358, 403, 550, 823
HpaII CCGG 3 cut(s) 407, 845, 911
HphI GGTGA 3 cut(s) 278, 446, 829
Hpy166II GTNNAC 5 cut(s) 14, 154, 269, 793, 898
Hpy188I TCNGA 7 cut(s) 20, 173, 402, 567, 574, 594, 989
Hpy188III TCNNGA 6 cut(s) 48, 258, 407, 473, 697, 911
Hpy8I GTNNAC 5 cut(s) 14, 154, 269, 793, 898
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 2 cut(s) 423, 617
HpyCH4IV ACGT 6 cut(s) 148, 283, 330, 349, 598, 875
HpyCH4V TGCA 7 cut(s) 14, 26, 72, 94, 236, 393, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 372
HpySE526I ACGT 6 cut(s) 148, 283, 330, 349, 598, 875
Hsp92II CATG 3 cut(s) 749, 754, 896
HspAI GCGC 2 cut(s) 467, 866
Kpn2I TCCGGA 2 cut(s) 406, 910
Kzo9I GATC 2 cut(s) 799, 922
LmnI GCTCC 2 cut(s) 809, 890
Lsp1109I GCAGC 2 cut(s) 10, 238
LweI GCATC 3 cut(s) 118, 775, 973
MaeI CTAG 5 cut(s) 185, 201, 738, 765, 926
MaeII ACGT 6 cut(s) 148, 283, 330, 349, 598, 875
MaeIII GTNAC 1 cut(s) 82
MalI GATC 2 cut(s) 801, 924
MboI GATC 2 cut(s) 799, 922
MboII GAAGA 1 cut(s) 457
MhlI GDGCHC 1 cut(s) 16
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 7 cut(s) 27, 344, 367, 499, 561, 568, 829
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 1 cut(s) 832
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 4 cut(s) 132, 265, 574, 813
Mox20I TGGCCA 1 cut(s) 632
Mph1103I ATGCAT 1 cut(s) 395
MroI TCCGGA 2 cut(s) 406, 910
MscI TGGCCA 1 cut(s) 632
MseI TTAA 4 cut(s) 104, 242, 428, 686
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 3 cut(s) 407, 845, 911
MspR9I CCNGG 2 cut(s) 80, 213
MvaI CCWGG 2 cut(s) 80, 213
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeII GATC 2 cut(s) 799, 922
NlaIII CATG 3 cut(s) 749, 754, 896
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 1 cut(s) 749
PcsI WCGNNNNNNNCGW 1 cut(s) 289
PfeI GAWTC 3 cut(s) 358, 403, 550
PkrI GCNGC 2 cut(s) 25, 253
PleI GAGTC 1 cut(s) 831
PmaCI CACGTG 1 cut(s) 149
PmlI CACGTG 1 cut(s) 149
PpsI GAGTC 1 cut(s) 831
Ppu21I YACGTR 2 cut(s) 149, 350
Psp6I CCWGG 2 cut(s) 78, 211
PspCI CACGTG 1 cut(s) 149
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 2 cut(s) 78, 211
PspPI GGNCC 2 cut(s) 36, 842
PvuII CAGCTG 1 cut(s) 463
RsaI GTAC 2 cut(s) 664, 775
RsaNI GTAC 2 cut(s) 663, 774
SaqAI TTAA 4 cut(s) 104, 242, 428, 686
SatI GCNGC 2 cut(s) 24, 252
Sau3AI GATC 2 cut(s) 799, 922
Sau96I GGNCC 2 cut(s) 36, 842
SchI GAGTC 1 cut(s) 832
ScrFI CCNGG 2 cut(s) 80, 213
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 3 cut(s) 118, 775, 973
SinI GGWCC 2 cut(s) 36, 842
SmlI CTYRAG 3 cut(s) 256, 658, 947
SmoI CTYRAG 3 cut(s) 256, 658, 947
Sse9I AATT 7 cut(s) 27, 344, 367, 499, 561, 568, 829
SspI AATATT 1 cut(s) 246
SspMI CTAG 5 cut(s) 185, 201, 738, 765, 926
StyD4I CCNGG 2 cut(s) 78, 211
StyI CCWWGG 4 cut(s) 39, 200, 481, 737
TaaI ACNGT 2 cut(s) 423, 617
TaiI ACGT 6 cut(s) 151, 286, 333, 352, 601, 878
TaqI TCGA 3 cut(s) 327, 342, 711
TasI AATT 7 cut(s) 27, 344, 367, 499, 561, 568, 829
TatI WGTACW 2 cut(s) 662, 773
TfiI GAWTC 3 cut(s) 358, 403, 550
Tru1I TTAA 4 cut(s) 104, 242, 428, 686
Tru9I TTAA 4 cut(s) 104, 242, 428, 686
TscAI CASTG 2 cut(s) 343, 633
TseI GCWGC 2 cut(s) 23, 251
TspDTI ATGAA 2 cut(s) 458, 638
TspGWI ACGGA 3 cut(s) 330, 593, 738
TspRI CASTG 2 cut(s) 343, 633
VneI GTGCAC 1 cut(s) 12
VpaK11BI GGWCC 2 cut(s) 36, 842
XapI RAATTY 3 cut(s) 367, 561, 568
XceI RCATGY 1 cut(s) 749
XcmI CCANNNNNNNNNTGG 2 cut(s) 460, 650
XmaJI CCTAGG 2 cut(s) 200, 737
XspI CTAG 5 cut(s) 185, 201, 738, 765, 926
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.